Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068646.t1 | ABN08740.1 | 86.4 | 59 | 8 | 0 | 84 | 142 | 156 | 214 | 1.50E-22 | 115.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068646.t1 | A2Q540 | 86.4 | 59 | 8 | 0 | 84 | 142 | 156 | 214 | 1.1e-22 | 115.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049254 | MS.gene068646 | 0.807456 | 5.01E-50 | -1.69E-46 |
| MS.gene049821 | MS.gene068646 | 0.813244 | 2.82E-51 | -1.69E-46 |
| MS.gene051198 | MS.gene068646 | 0.805464 | 1.32E-49 | -1.69E-46 |
| MS.gene051247 | MS.gene068646 | 0.802193 | 6.30E-49 | -1.69E-46 |
| MS.gene052519 | MS.gene068646 | 0.807538 | 4.81E-50 | -1.69E-46 |
| MS.gene052582 | MS.gene068646 | 0.808416 | 3.13E-50 | -1.69E-46 |
| MS.gene052584 | MS.gene068646 | 0.822158 | 2.75E-53 | -1.69E-46 |
| MS.gene052773 | MS.gene068646 | 0.800725 | 1.26E-48 | -1.69E-46 |
| MS.gene053122 | MS.gene068646 | 0.800194 | 1.62E-48 | -1.69E-46 |
| MS.gene053226 | MS.gene068646 | 0.805716 | 1.17E-49 | -1.69E-46 |
| MS.gene053883 | MS.gene068646 | 0.807028 | 6.17E-50 | -1.69E-46 |
| MS.gene05415 | MS.gene068646 | 0.803646 | 3.16E-49 | -1.69E-46 |
| MS.gene054651 | MS.gene068646 | 0.814271 | 1.68E-51 | -1.69E-46 |
| MS.gene054775 | MS.gene068646 | 0.808014 | 3.81E-50 | -1.69E-46 |
| MS.gene054991 | MS.gene068646 | 0.815717 | 8.01E-52 | -1.69E-46 |
| MS.gene055465 | MS.gene068646 | 0.80861 | 2.85E-50 | -1.69E-46 |
| MS.gene055466 | MS.gene068646 | 0.81507 | 1.12E-51 | -1.69E-46 |
| MS.gene055468 | MS.gene068646 | 0.809698 | 1.66E-50 | -1.69E-46 |
| MS.gene056174 | MS.gene068646 | 0.806468 | 8.11E-50 | -1.69E-46 |
| MS.gene056308 | MS.gene068646 | 0.82253 | 2.26E-53 | -1.69E-46 |
| MS.gene057444 | MS.gene068646 | 0.839164 | 1.87E-57 | -1.69E-46 |
| MS.gene058625 | MS.gene068646 | 0.805096 | 1.57E-49 | -1.69E-46 |
| MS.gene059460 | MS.gene068646 | 0.80484 | 1.78E-49 | -1.69E-46 |
| MS.gene059625 | MS.gene068646 | 0.845856 | 3.13E-59 | -1.69E-46 |
| MS.gene060429 | MS.gene068646 | 0.830658 | 2.59E-55 | -1.69E-46 |
| MS.gene060634 | MS.gene068646 | -0.805874 | 1.08E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068646.t1 | MTR_7g076850 | 90.769 | 65 | 5 | 1 | 51 | 115 | 124 | 187 | 9.06e-34 | 122 |
| MS.gene068646.t1 | MTR_5g030750 | 72.269 | 119 | 22 | 2 | 2 | 116 | 73 | 184 | 2.17e-26 | 102 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 40 sgRNAs with CRISPR-Local
Find 69 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAAAACCTAGTCCTCATTTC+TGG | 0.236228 | 5.1:+21041271 | MS.gene068646:CDS |
| GAGATTGAAGGTGGAGTAGA+AGG | 0.245379 | 5.1:+21041090 | MS.gene068646:CDS |
| CTGGAAGAACGTTTCGAAAC+TGG | 0.311757 | 5.1:+21041290 | MS.gene068646:CDS |
| TGGTTGAAGGTGGTAGAAAA+AGG | 0.424066 | 5.1:+21041019 | MS.gene068646:CDS |
| GAAAAGAGAAGAAAAGTAGC+GGG | 0.430036 | 5.1:+21040989 | MS.gene068646:CDS |
| AAGGTGGAGTAGAAGGGAAA+AGG | 0.444952 | 5.1:+21041097 | MS.gene068646:CDS |
| GTGGACTAAGGAACAAGAAT+TGG | 0.455229 | 5.1:+21041224 | MS.gene068646:CDS |
| GAAACGTTCTTCCAGAAATG+AGG | 0.491137 | 5.1:-21041282 | None:intergenic |
| CGATGCTGGTGGTGAAATAG+TGG | 0.492076 | 5.1:+21041125 | MS.gene068646:CDS |
| AGAAGAAAGCACGGTGGTGA+TGG | 0.502654 | 5.1:+21041042 | MS.gene068646:CDS |
| GGGAAAAGGAAGCACGATGC+TGG | 0.520506 | 5.1:+21041111 | MS.gene068646:CDS |
| AGGAAGAGAAAGCGCGGTGG+TGG | 0.525493 | 5.1:+21041186 | MS.gene068646:CDS |
| AAGAAAGAAGAGATTGAAGG+TGG | 0.529397 | 5.1:+21041081 | MS.gene068646:CDS |
| AATAGTGGAAGAGTTGAGGA+AGG | 0.535399 | 5.1:+21041140 | MS.gene068646:CDS |
| GGAAAAGAGAAGAAAAGTAG+CGG | 0.543653 | 5.1:+21040988 | MS.gene068646:CDS |
| AGGAAGAAAGAAGAGATTGA+AGG | 0.543684 | 5.1:+21041078 | MS.gene068646:CDS |
| GTGAAATAGTGGAAGAGTTG+AGG | 0.549461 | 5.1:+21041136 | MS.gene068646:CDS |
| AGATTGAAGGTGGAGTAGAA+GGG | 0.553424 | 5.1:+21041091 | MS.gene068646:CDS |
| AAAAGAGAAGAAAAGTAGCG+GGG | 0.557669 | 5.1:+21040990 | MS.gene068646:CDS |
| GCACGGTGGTGATGGAATTG+AGG | 0.558489 | 5.1:+21041050 | MS.gene068646:CDS |
| TTCTTCCAGAAATGAGGACT+AGG | 0.561243 | 5.1:-21041276 | None:intergenic |
| AAGTTGAATGTGGTGTGAAA+AGG | 0.575392 | 5.1:+21041166 | MS.gene068646:CDS |
| GTGATGGAATTGAGGAAGGG+AGG | 0.575565 | 5.1:+21041058 | MS.gene068646:CDS |
| GGTGGTGATGGAATTGAGGA+AGG | 0.582281 | 5.1:+21041054 | MS.gene068646:CDS |
| GCTGCACACAATGATAAAGA+AGG | 0.583596 | 5.1:+21041967 | MS.gene068646:CDS |
| ACTTGCACAAGGGTGGACTA+AGG | 0.584727 | 5.1:+21041212 | MS.gene068646:CDS |
| AAAGAGAAGAAAAGTAGCGG+GGG | 0.585434 | 5.1:+21040991 | MS.gene068646:CDS |
| CATTGCAGCATGGCTTGCAG+TGG | 0.590066 | 5.1:+21041990 | MS.gene068646:CDS |
| TGGTAGAGTCGCACGCAGTG+TGG | 0.627677 | 5.1:+21042010 | MS.gene068646:CDS |
| GTGGTGGTGAACTTGCACAA+GGG | 0.638118 | 5.1:+21041202 | MS.gene068646:CDS |
| AAAAGGAAGCACGATGCTGG+TGG | 0.639531 | 5.1:+21041114 | MS.gene068646:CDS |
| GGTGGTGGTGAACTTGCACA+AGG | 0.641123 | 5.1:+21041201 | MS.gene068646:CDS |
| CATTGTGTGCAGCATCGCAG+CGG | 0.644173 | 5.1:-21041957 | None:intergenic |
| GTGGTGAACTTGCACAAGGG+TGG | 0.659011 | 5.1:+21041205 | MS.gene068646:CDS |
| ATAAAGAAGGCATTGCAGCA+TGG | 0.664960 | 5.1:+21041980 | MS.gene068646:CDS |
| GTGGTGATGGAATTGAGGAA+GGG | 0.666606 | 5.1:+21041055 | MS.gene068646:CDS |
| AGGAAGGAAGAAGTTGAATG+TGG | 0.671131 | 5.1:+21041156 | MS.gene068646:CDS |
| GTGAAAAGGAAGAGAAAGCG+CGG | 0.698757 | 5.1:+21041180 | MS.gene068646:CDS |
| AAAAGGAAGAGAAAGCGCGG+TGG | 0.749477 | 5.1:+21041183 | MS.gene068646:CDS |
| AAAAGGAGAAGAAAGCACGG+TGG | 0.843786 | 5.1:+21041036 | MS.gene068646:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TGATATGTGACATAATATTA+TGG | + | chr5.1:21041491-21041510 | MS.gene068646:intron | 20.0% |
| ! | AAACTGTGAAGAAGATTAAT+GGG | + | chr5.1:21041455-21041474 | MS.gene068646:intron | 25.0% |
| ! | AGACAGTATTTAGTGTTATA+AGG | + | chr5.1:21041534-21041553 | MS.gene068646:intron | 25.0% |
| ! | GAAAAACAACAACAAAACAA+AGG | - | chr5.1:21041886-21041905 | None:intergenic | 25.0% |
| ! | GTATTTAGTGTTATAAGGTT+TGG | + | chr5.1:21041539-21041558 | MS.gene068646:intron | 25.0% |
| ! | TTGAATTATGTGTTTAAACG+AGG | + | chr5.1:21041409-21041428 | MS.gene068646:intron | 25.0% |
| !! | AGTGATTAATTTTGAGCAAA+TGG | - | chr5.1:21041823-21041842 | None:intergenic | 25.0% |
| !! | TTCAGTAAGCTAAATTTGTT+TGG | + | chr5.1:21041594-21041613 | MS.gene068646:intron | 25.0% |
| ACTAAATACTGTCTACTACA+TGG | - | chr5.1:21041528-21041547 | None:intergenic | 30.0% | |
| CAACACTATTAGTAAATAGC+TGG | + | chr5.1:21041657-21041676 | MS.gene068646:intron | 30.0% | |
| GAAACTGTGAAGAAGATTAA+TGG | + | chr5.1:21041454-21041473 | MS.gene068646:intron | 30.0% | |
| TCTTACATACAGAGAAAGAT+AGG | - | chr5.1:21041321-21041340 | None:intergenic | 30.0% | |
| ! | CAAAACAAAGGCATAAGATA+AGG | - | chr5.1:21041874-21041893 | None:intergenic | 30.0% |
| ! | CTACTAACTAGTTTAAACCT+AGG | - | chr5.1:21041692-21041711 | None:intergenic | 30.0% |
| !!! | ATGACGATTTTGGTTTATCA+CGG | + | chr5.1:21041848-21041867 | MS.gene068646:intron | 30.0% |
| AAAAGAGAAGAAAAGTAGCG+GGG | + | chr5.1:21040990-21041009 | MS.gene068646:CDS | 35.0% | |
| AAATTCGTCAAGTATCAGCA+AGG | + | chr5.1:21041632-21041651 | MS.gene068646:intron | 35.0% | |
| AAGAAAGAAGAGATTGAAGG+TGG | + | chr5.1:21041081-21041100 | MS.gene068646:CDS | 35.0% | |
| AAGTTGAATGTGGTGTGAAA+AGG | + | chr5.1:21041166-21041185 | MS.gene068646:CDS | 35.0% | |
| AGAAAAAGGAGAAGAAAGCA+CGG | + | chr5.1:21041033-21041052 | MS.gene068646:CDS | 35.0% | |
| AGGAAGAAAGAAGAGATTGA+AGG | + | chr5.1:21041078-21041097 | MS.gene068646:CDS | 35.0% | |
| AGTAGTGTGTATGTCTAGAA+AGG | + | chr5.1:21041707-21041726 | MS.gene068646:intron | 35.0% | |
| GAAAAGAGAAGAAAAGTAGC+GGG | + | chr5.1:21040989-21041008 | MS.gene068646:CDS | 35.0% | |
| GGAAAAGAGAAGAAAAGTAG+CGG | + | chr5.1:21040988-21041007 | MS.gene068646:CDS | 35.0% | |
| ! | CTGTTTTCACATGACATTTC+AGG | - | chr5.1:21041932-21041951 | None:intergenic | 35.0% |
| ! | TCACTCAAAGATGACGATTT+TGG | + | chr5.1:21041838-21041857 | MS.gene068646:intron | 35.0% |
| ! | TGTGAAGAAGATTAATGGGT+TGG | + | chr5.1:21041459-21041478 | MS.gene068646:intron | 35.0% |
| AAAGAGAAGAAAAGTAGCGG+GGG | + | chr5.1:21040991-21041010 | MS.gene068646:CDS | 40.0% | |
| ACTAATCTGCGCTGAGATTA+AGG | - | chr5.1:21041764-21041783 | None:intergenic | 40.0% | |
| AGATTGAAGGTGGAGTAGAA+GGG | + | chr5.1:21041091-21041110 | MS.gene068646:CDS | 40.0% | |
| AGGAAGGAAGAAGTTGAATG+TGG | + | chr5.1:21041156-21041175 | MS.gene068646:CDS | 40.0% | |
| ATAGCTGGATAATGTGTCCT+AGG | + | chr5.1:21041672-21041691 | MS.gene068646:intron | 40.0% | |
| ATGAGAAAAACAGGTCGTGA+AGG | + | chr5.1:21040967-21040986 | MS.gene068646:CDS | 40.0% | |
| ATGTCTAGAAAGGTCTTCTC+AGG | + | chr5.1:21041717-21041736 | MS.gene068646:intron | 40.0% | |
| CAAAACCTAGTCCTCATTTC+TGG | + | chr5.1:21041271-21041290 | MS.gene068646:CDS | 40.0% | |
| GAAACGTTCTTCCAGAAATG+AGG | - | chr5.1:21041285-21041304 | None:intergenic | 40.0% | |
| GCTGCACACAATGATAAAGA+AGG | + | chr5.1:21041967-21041986 | MS.gene068646:CDS | 40.0% | |
| TGGTTGAAGGTGGTAGAAAA+AGG | + | chr5.1:21041019-21041038 | MS.gene068646:CDS | 40.0% | |
| TTCTTCCAGAAATGAGGACT+AGG | - | chr5.1:21041279-21041298 | None:intergenic | 40.0% | |
| ! | AATAGTGGAAGAGTTGAGGA+AGG | + | chr5.1:21041140-21041159 | MS.gene068646:CDS | 40.0% |
| ! | GTGAAATAGTGGAAGAGTTG+AGG | + | chr5.1:21041136-21041155 | MS.gene068646:CDS | 40.0% |
| ! | GTGGACTAAGGAACAAGAAT+TGG | + | chr5.1:21041224-21041243 | MS.gene068646:CDS | 40.0% |
| !! | ATAAAGAAGGCATTGCAGCA+TGG | + | chr5.1:21041980-21041999 | MS.gene068646:CDS | 40.0% |
| AAGGTGGAGTAGAAGGGAAA+AGG | + | chr5.1:21041097-21041116 | MS.gene068646:CDS | 45.0% | |
| CTGGAAGAACGTTTCGAAAC+TGG | + | chr5.1:21041290-21041309 | MS.gene068646:CDS | 45.0% | |
| GAGATTGAAGGTGGAGTAGA+AGG | + | chr5.1:21041090-21041109 | MS.gene068646:CDS | 45.0% | |
| GTGAAAAGGAAGAGAAAGCG+CGG | + | chr5.1:21041180-21041199 | MS.gene068646:CDS | 45.0% | |
| TCTCAGGAGAGTCAAAACAC+AGG | + | chr5.1:21041733-21041752 | MS.gene068646:intron | 45.0% | |
| ! | AAAAGGAGAAGAAAGCACGG+TGG | + | chr5.1:21041036-21041055 | MS.gene068646:CDS | 45.0% |
| !! | GTGGTGATGGAATTGAGGAA+GGG | + | chr5.1:21041055-21041074 | MS.gene068646:CDS | 45.0% |
| ! | AAAAGGAAGAGAAAGCGCGG+TGG | + | chr5.1:21041183-21041202 | MS.gene068646:CDS | 50.0% |
| ! | AAAAGGAAGCACGATGCTGG+TGG | + | chr5.1:21041114-21041133 | MS.gene068646:CDS | 50.0% |
| ! | AGAAGAAAGCACGGTGGTGA+TGG | + | chr5.1:21041042-21041061 | MS.gene068646:CDS | 50.0% |
| ! | CGATGCTGGTGGTGAAATAG+TGG | + | chr5.1:21041125-21041144 | MS.gene068646:CDS | 50.0% |
| ! | GTGATGGAATTGAGGAAGGG+AGG | + | chr5.1:21041058-21041077 | MS.gene068646:CDS | 50.0% |
| ! | GTGGTGGTGAACTTGCACAA+GGG | + | chr5.1:21041202-21041221 | MS.gene068646:CDS | 50.0% |
| !! | ACTTGCACAAGGGTGGACTA+AGG | + | chr5.1:21041212-21041231 | MS.gene068646:CDS | 50.0% |
| !! | GGTGGTGATGGAATTGAGGA+AGG | + | chr5.1:21041054-21041073 | MS.gene068646:CDS | 50.0% |
| CATTGTGTGCAGCATCGCAG+CGG | - | chr5.1:21041960-21041979 | None:intergenic | 55.0% | |
| GTGGTGAACTTGCACAAGGG+TGG | + | chr5.1:21041205-21041224 | MS.gene068646:CDS | 55.0% | |
| ! | CATTGCAGCATGGCTTGCAG+TGG | + | chr5.1:21041990-21042009 | MS.gene068646:CDS | 55.0% |
| ! | GAAAAGTAGCGGGGGAGTTG+TGG | + | chr5.1:21040999-21041018 | MS.gene068646:CDS | 55.0% |
| ! | GGGAAAAGGAAGCACGATGC+TGG | + | chr5.1:21041111-21041130 | MS.gene068646:CDS | 55.0% |
| ! | GGTGGTGGTGAACTTGCACA+AGG | + | chr5.1:21041201-21041220 | MS.gene068646:CDS | 55.0% |
| !! | GCACGGTGGTGATGGAATTG+AGG | + | chr5.1:21041050-21041069 | MS.gene068646:CDS | 55.0% |
| AGCGGGGGAGTTGTGGTTGA+AGG | + | chr5.1:21041006-21041025 | MS.gene068646:CDS | 60.0% | |
| GGGGGAGTTGTGGTTGAAGG+TGG | + | chr5.1:21041009-21041028 | MS.gene068646:CDS | 60.0% | |
| TGGTAGAGTCGCACGCAGTG+TGG | + | chr5.1:21042010-21042029 | MS.gene068646:CDS | 60.0% | |
| ! | AGGAAGAGAAAGCGCGGTGG+TGG | + | chr5.1:21041186-21041205 | MS.gene068646:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 21040967 | 21042041 | 21040967 | ID=MS.gene068646 |
| chr5.1 | mRNA | 21040967 | 21042041 | 21040967 | ID=MS.gene068646.t1;Parent=MS.gene068646 |
| chr5.1 | exon | 21040967 | 21041311 | 21040967 | ID=MS.gene068646.t1.exon1;Parent=MS.gene068646.t1 |
| chr5.1 | CDS | 21040967 | 21041311 | 21040967 | ID=cds.MS.gene068646.t1;Parent=MS.gene068646.t1 |
| chr5.1 | exon | 21041952 | 21042041 | 21041952 | ID=MS.gene068646.t1.exon2;Parent=MS.gene068646.t1 |
| chr5.1 | CDS | 21041952 | 21042041 | 21041952 | ID=cds.MS.gene068646.t1;Parent=MS.gene068646.t1 |
| Gene Sequence |
| Protein sequence |
