Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07352.t1 | XP_003591331.1 | 97.5 | 121 | 3 | 0 | 1 | 121 | 1 | 121 | 5.30E-61 | 243.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07352.t1 | Q8S925 | 69.6 | 112 | 34 | 0 | 7 | 118 | 8 | 119 | 1.8e-41 | 169.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07352.t1 | G7IBT1 | 97.5 | 121 | 3 | 0 | 1 | 121 | 1 | 121 | 3.8e-61 | 243.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04915 | MS.gene07352 | -0.826272 | 2.97E-54 | -1.69E-46 |
| MS.gene049178 | MS.gene07352 | -0.802932 | 4.44E-49 | -1.69E-46 |
| MS.gene04917 | MS.gene07352 | -0.818325 | 2.08E-52 | -1.69E-46 |
| MS.gene049758 | MS.gene07352 | -0.824902 | 6.28E-54 | -1.69E-46 |
| MS.gene050226 | MS.gene07352 | -0.809245 | 2.08E-50 | -1.69E-46 |
| MS.gene050227 | MS.gene07352 | -0.80917 | 2.16E-50 | -1.69E-46 |
| MS.gene050228 | MS.gene07352 | -0.804072 | 2.57E-49 | -1.69E-46 |
| MS.gene050623 | MS.gene07352 | 0.847259 | 1.29E-59 | -1.69E-46 |
| MS.gene050629 | MS.gene07352 | 0.844816 | 5.98E-59 | -1.69E-46 |
| MS.gene050762 | MS.gene07352 | -0.833587 | 4.90E-56 | -1.69E-46 |
| MS.gene050763 | MS.gene07352 | -0.835404 | 1.71E-56 | -1.69E-46 |
| MS.gene051662 | MS.gene07352 | -0.810396 | 1.18E-50 | -1.69E-46 |
| MS.gene051663 | MS.gene07352 | -0.803327 | 3.68E-49 | -1.69E-46 |
| MS.gene052115 | MS.gene07352 | -0.832977 | 6.95E-56 | -1.69E-46 |
| MS.gene052309 | MS.gene07352 | 0.805534 | 1.27E-49 | -1.69E-46 |
| MS.gene052376 | MS.gene07352 | -0.800011 | 1.76E-48 | -1.69E-46 |
| MS.gene052420 | MS.gene07352 | -0.809475 | 1.86E-50 | -1.69E-46 |
| MS.gene053836 | MS.gene07352 | -0.825815 | 3.82E-54 | -1.69E-46 |
| MS.gene054379 | MS.gene07352 | -0.837551 | 4.86E-57 | -1.69E-46 |
| MS.gene054930 | MS.gene07352 | -0.834307 | 3.23E-56 | -1.69E-46 |
| MS.gene055174 | MS.gene07352 | -0.805727 | 1.16E-49 | -1.69E-46 |
| MS.gene055816 | MS.gene07352 | 0.835635 | 1.50E-56 | -1.69E-46 |
| MS.gene056069 | MS.gene07352 | 0.80157 | 8.46E-49 | -1.69E-46 |
| MS.gene05761 | MS.gene07352 | -0.808239 | 3.42E-50 | -1.69E-46 |
| MS.gene057665 | MS.gene07352 | -0.815649 | 8.29E-52 | -1.69E-46 |
| MS.gene058182 | MS.gene07352 | -0.812706 | 3.70E-51 | -1.69E-46 |
| MS.gene058661 | MS.gene07352 | 0.841222 | 5.42E-58 | -1.69E-46 |
| MS.gene058851 | MS.gene07352 | -0.804683 | 1.92E-49 | -1.69E-46 |
| MS.gene060084 | MS.gene07352 | -0.832336 | 1.00E-55 | -1.69E-46 |
| MS.gene060802 | MS.gene07352 | -0.806546 | 7.80E-50 | -1.69E-46 |
| MS.gene060869 | MS.gene07352 | -0.836629 | 8.37E-57 | -1.69E-46 |
| MS.gene060870 | MS.gene07352 | -0.810538 | 1.10E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07352.t1 | MTR_1g086310 | 97.521 | 121 | 3 | 0 | 1 | 121 | 1 | 121 | 9.28e-86 | 245 |
| MS.gene07352.t1 | MTR_4g123760 | 68.644 | 118 | 37 | 0 | 1 | 118 | 1 | 118 | 2.78e-59 | 178 |
| MS.gene07352.t1 | MTR_2g023430 | 54.310 | 116 | 53 | 0 | 5 | 120 | 4 | 119 | 1.93e-43 | 138 |
| MS.gene07352.t1 | MTR_4g048510 | 54.310 | 116 | 53 | 0 | 3 | 118 | 2 | 117 | 2.01e-43 | 138 |
| MS.gene07352.t1 | MTR_4g101090 | 52.586 | 116 | 55 | 0 | 5 | 120 | 4 | 119 | 3.07e-41 | 133 |
| MS.gene07352.t1 | MTR_2g088230 | 48.246 | 114 | 47 | 1 | 5 | 118 | 4 | 105 | 6.23e-33 | 111 |
| MS.gene07352.t1 | MTR_7g096540 | 74.194 | 62 | 16 | 0 | 57 | 118 | 1 | 62 | 1.86e-29 | 101 |
| MS.gene07352.t1 | MTR_4g037225 | 45.536 | 112 | 59 | 2 | 6 | 115 | 5 | 116 | 7.87e-27 | 96.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07352.t1 | AT3G06420 | 69.027 | 113 | 35 | 0 | 6 | 118 | 7 | 119 | 8.25e-57 | 172 |
| MS.gene07352.t1 | AT3G15580 | 70.536 | 112 | 33 | 0 | 7 | 118 | 4 | 115 | 1.10e-56 | 172 |
| MS.gene07352.t1 | AT4G21980 | 57.265 | 117 | 50 | 0 | 5 | 121 | 4 | 120 | 4.80e-45 | 142 |
| MS.gene07352.t1 | AT4G21980 | 57.265 | 117 | 50 | 0 | 5 | 121 | 19 | 135 | 1.14e-44 | 142 |
| MS.gene07352.t1 | AT1G62040 | 56.897 | 116 | 50 | 0 | 3 | 118 | 2 | 117 | 2.12e-44 | 140 |
| MS.gene07352.t1 | AT2G45170 | 53.719 | 121 | 56 | 0 | 1 | 121 | 1 | 121 | 3.16e-44 | 140 |
| MS.gene07352.t1 | AT2G45170 | 53.719 | 121 | 56 | 0 | 1 | 121 | 1 | 121 | 3.16e-44 | 140 |
| MS.gene07352.t1 | AT4G04620 | 53.448 | 116 | 54 | 0 | 5 | 120 | 4 | 119 | 2.63e-42 | 135 |
| MS.gene07352.t1 | AT4G04620 | 53.448 | 116 | 54 | 0 | 5 | 120 | 4 | 119 | 2.63e-42 | 135 |
| MS.gene07352.t1 | AT4G04620 | 53.448 | 116 | 54 | 0 | 5 | 120 | 4 | 119 | 2.63e-42 | 135 |
| MS.gene07352.t1 | AT2G05630 | 53.509 | 114 | 53 | 0 | 5 | 118 | 4 | 117 | 5.94e-42 | 134 |
| MS.gene07352.t1 | AT4G16520 | 54.386 | 114 | 52 | 0 | 5 | 118 | 4 | 117 | 1.41e-41 | 134 |
| MS.gene07352.t1 | AT4G16520 | 54.386 | 114 | 52 | 0 | 5 | 118 | 4 | 117 | 1.41e-41 | 134 |
| MS.gene07352.t1 | AT3G60640 | 52.174 | 115 | 55 | 0 | 6 | 120 | 5 | 119 | 5.11e-41 | 132 |
| MS.gene07352.t1 | AT2G05630 | 52.778 | 108 | 51 | 0 | 11 | 118 | 54 | 161 | 1.34e-38 | 127 |
| MS.gene07352.t1 | AT4G16520 | 55.682 | 88 | 39 | 0 | 31 | 118 | 30 | 117 | 1.02e-30 | 106 |
| MS.gene07352.t1 | AT1G62040 | 52.688 | 93 | 44 | 0 | 3 | 95 | 2 | 94 | 1.55e-29 | 102 |
Find 22 sgRNAs with CRISPR-Local
Find 36 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGGGATTCGATCGGGGTATT+TGG | 0.168599 | 1.2:-65023634 | None:intergenic |
| TACCAAACGTAAACTCGTTC+TGG | 0.338661 | 1.2:-65023497 | None:intergenic |
| TCCAGCTGGTAAATTAAGTC+TGG | 0.354424 | 1.2:-65023970 | None:intergenic |
| CAAATGCGATCTGCCTCATT+TGG | 0.355422 | 1.2:+65023765 | MS.gene07352:CDS |
| AAGAATACTTTACCTCAAAC+TGG | 0.387404 | 1.2:+65024014 | MS.gene07352:CDS |
| GGACTTACGGGGATTCGATC+GGG | 0.390336 | 1.2:-65023642 | None:intergenic |
| TATAGCACTGAGAAAACCTT+TGG | 0.412532 | 1.2:+65024194 | MS.gene07352:CDS |
| TGCTATTGCAGCTAGTATGA+TGG | 0.424492 | 1.2:+65024121 | MS.gene07352:intron |
| GCAAGTTTATTACCAGTTTG+AGG | 0.425620 | 1.2:-65024026 | None:intergenic |
| CAAATGAGGCAGATCGCATT+TGG | 0.427187 | 1.2:-65023764 | None:intergenic |
| AATAGTTGACATTTATGTAC+AGG | 0.446245 | 1.2:-65024219 | None:intergenic |
| TCCAGACTTAATTTACCAGC+TGG | 0.456823 | 1.2:+65023969 | MS.gene07352:CDS |
| TCTTCTTCTTCAGGTGATTG+TGG | 0.457646 | 1.2:+65023732 | MS.gene07352:intron |
| CTCTGTTTATAGATCATTCA+AGG | 0.464700 | 1.2:+65024145 | MS.gene07352:CDS |
| TTCCAGAACGAGTTTACGTT+TGG | 0.466165 | 1.2:+65023495 | MS.gene07352:CDS |
| GGGACTTACGGGGATTCGAT+CGG | 0.493031 | 1.2:-65023643 | None:intergenic |
| TTATAGATCATTCAAGGATG+AGG | 0.555700 | 1.2:+65024151 | MS.gene07352:CDS |
| AGATCATTCAAGGATGAGGA+TGG | 0.557576 | 1.2:+65024155 | MS.gene07352:CDS |
| CATTTATGTACAGGATCCAA+AGG | 0.566947 | 1.2:-65024210 | None:intergenic |
| GTTCCACGAGACTTGTCAGT+TGG | 0.569472 | 1.2:+65023924 | MS.gene07352:CDS |
| GACTTACGGGGATTCGATCG+GGG | 0.607489 | 1.2:-65023641 | None:intergenic |
| TGTCCAACTGACAAGTCTCG+TGG | 0.654531 | 1.2:-65023927 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAAAAAATAGATTTAAGATT+AGG | - | chr1.2:65024057-65024076 | None:intergenic | 10.0% |
| !! | AAGCCAAATTGAAATTAAAT+TGG | - | chr1.2:65023694-65023713 | None:intergenic | 20.0% |
| !!! | AATAATTTTTCTTCTTCTTC+AGG | + | chr1.2:65023723-65023742 | MS.gene07352:intron | 20.0% |
| ! | ATACGAAAATCAATGATTGA+TGG | - | chr1.2:65023667-65023686 | None:intergenic | 25.0% |
| ! | CCAAATTGAAATTAAATTGG+AGG | - | chr1.2:65023691-65023710 | None:intergenic | 25.0% |
| ! | TACGAAAATCAATGATTGAT+GGG | - | chr1.2:65023666-65023685 | None:intergenic | 25.0% |
| !! | CCTCCAATTTAATTTCAATT+TGG | + | chr1.2:65023688-65023707 | MS.gene07352:intron | 25.0% |
| AAGAATACTTTACCTCAAAC+TGG | + | chr1.2:65024014-65024033 | MS.gene07352:CDS | 30.0% | |
| CTCTGTTTATAGATCATTCA+AGG | + | chr1.2:65024145-65024164 | MS.gene07352:CDS | 30.0% | |
| TTATAGATCATTCAAGGATG+AGG | + | chr1.2:65024151-65024170 | MS.gene07352:CDS | 30.0% | |
| ! | TTTTCTCAGTGCTATAATAC+AGG | - | chr1.2:65024190-65024209 | None:intergenic | 30.0% |
| !!! | TACTTTTTCTTCTCCAAATG+AGG | - | chr1.2:65023781-65023800 | None:intergenic | 30.0% |
| CAACATGAAAAAGCAACAAG+AGG | - | chr1.2:65024088-65024107 | None:intergenic | 35.0% | |
| GCAAGTTTATTACCAGTTTG+AGG | - | chr1.2:65024029-65024048 | None:intergenic | 35.0% | |
| TCAATGATTGATGGGACTTA+CGG | - | chr1.2:65023658-65023677 | None:intergenic | 35.0% | |
| ! | TATAGCACTGAGAAAACCTT+TGG | + | chr1.2:65024194-65024213 | MS.gene07352:CDS | 35.0% |
| AATGATTGATGGGACTTACG+GGG | - | chr1.2:65023656-65023675 | None:intergenic | 40.0% | |
| AGATCATTCAAGGATGAGGA+TGG | + | chr1.2:65024155-65024174 | MS.gene07352:CDS | 40.0% | |
| CAATGATTGATGGGACTTAC+GGG | - | chr1.2:65023657-65023676 | None:intergenic | 40.0% | |
| TACCAAACGTAAACTCGTTC+TGG | - | chr1.2:65023500-65023519 | None:intergenic | 40.0% | |
| TCCAGACTTAATTTACCAGC+TGG | + | chr1.2:65023969-65023988 | MS.gene07352:CDS | 40.0% | |
| TCCAGCTGGTAAATTAAGTC+TGG | - | chr1.2:65023973-65023992 | None:intergenic | 40.0% | |
| TCTTCTTCTTCAGGTGATTG+TGG | + | chr1.2:65023732-65023751 | MS.gene07352:intron | 40.0% | |
| TGCTATTGCAGCTAGTATGA+TGG | + | chr1.2:65024121-65024140 | MS.gene07352:intron | 40.0% | |
| TTCCAGAACGAGTTTACGTT+TGG | + | chr1.2:65023495-65023514 | MS.gene07352:CDS | 40.0% | |
| ! | TTTTCGCGTGTGTAGAACAA+AGG | + | chr1.2:65023586-65023605 | MS.gene07352:intron | 40.0% |
| CAAATGAGGCAGATCGCATT+TGG | - | chr1.2:65023767-65023786 | None:intergenic | 45.0% | |
| CAAATGCGATCTGCCTCATT+TGG | + | chr1.2:65023765-65023784 | MS.gene07352:CDS | 45.0% | |
| ! | ACAAAGAGAGCTTTTCCAGC+TGG | - | chr1.2:65023987-65024006 | None:intergenic | 45.0% |
| !! | ATAAGATAAAATTATTAAAA+AGG | - | chr1.2:65023538-65023557 | None:intergenic | 5.0% |
| GTTCCACGAGACTTGTCAGT+TGG | + | chr1.2:65023924-65023943 | MS.gene07352:CDS | 50.0% | |
| TGTCCAACTGACAAGTCTCG+TGG | - | chr1.2:65023930-65023949 | None:intergenic | 50.0% | |
| GACTTACGGGGATTCGATCG+GGG | - | chr1.2:65023644-65023663 | None:intergenic | 55.0% | |
| GGACTTACGGGGATTCGATC+GGG | - | chr1.2:65023645-65023664 | None:intergenic | 55.0% | |
| GGGACTTACGGGGATTCGAT+CGG | - | chr1.2:65023646-65023665 | None:intergenic | 55.0% | |
| ! | GGGGATTCGATCGGGGTATT+TGG | - | chr1.2:65023637-65023656 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 65023477 | 65024229 | 65023477 | ID=MS.gene07352 |
| chr1.2 | mRNA | 65023477 | 65024229 | 65023477 | ID=MS.gene07352.t1;Parent=MS.gene07352 |
| chr1.2 | exon | 65023477 | 65023516 | 65023477 | ID=MS.gene07352.t1.exon1;Parent=MS.gene07352.t1 |
| chr1.2 | CDS | 65023477 | 65023516 | 65023477 | ID=cds.MS.gene07352.t1;Parent=MS.gene07352.t1 |
| chr1.2 | exon | 65023601 | 65023656 | 65023601 | ID=MS.gene07352.t1.exon2;Parent=MS.gene07352.t1 |
| chr1.2 | CDS | 65023601 | 65023656 | 65023601 | ID=cds.MS.gene07352.t1;Parent=MS.gene07352.t1 |
| chr1.2 | exon | 65023745 | 65023797 | 65023745 | ID=MS.gene07352.t1.exon3;Parent=MS.gene07352.t1 |
| chr1.2 | CDS | 65023745 | 65023797 | 65023745 | ID=cds.MS.gene07352.t1;Parent=MS.gene07352.t1 |
| chr1.2 | exon | 65023917 | 65024035 | 65023917 | ID=MS.gene07352.t1.exon4;Parent=MS.gene07352.t1 |
| chr1.2 | CDS | 65023917 | 65024035 | 65023917 | ID=cds.MS.gene07352.t1;Parent=MS.gene07352.t1 |
| chr1.2 | exon | 65024132 | 65024229 | 65024132 | ID=MS.gene07352.t1.exon5;Parent=MS.gene07352.t1 |
| chr1.2 | CDS | 65024132 | 65024229 | 65024132 | ID=cds.MS.gene07352.t1;Parent=MS.gene07352.t1 |
| Gene Sequence |
| Protein sequence |
