AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene073610


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene073610.t1 MTR_1g050472 99.697 330 1 0 1 330 1 330 0.0 679
MS.gene073610.t1 MTR_0268s0080 97.273 330 9 0 1 330 1 330 0.0 670
MS.gene073610.t1 MTR_7g029110 96.364 330 12 0 1 330 1 330 0.0 663
MS.gene073610.t1 MTR_8g469010 92.727 330 19 1 1 330 1 325 0.0 635
MS.gene073610.t1 MTR_4g117370 76.615 325 75 1 6 330 18 341 0.0 534
MS.gene073610.t1 MTR_6g005270 67.442 43 14 0 265 307 25 67 1.58e-13 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene073610.t1 AT3G27060 83.735 332 52 2 1 330 1 332 0.0 558
MS.gene073610.t1 AT3G23580 76.308 325 76 1 6 330 18 341 0.0 518

Find 65 sgRNAs with CRISPR-Local

Find 85 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCGCGTTGGTTGGGATGAA+TGG 0.235467 1.1:+34647958 MS.gene073610:CDS
TGCAGAATCGGTCTGGGTTT+GGG 0.237047 1.1:-34647255 None:intergenic
CGCTTCCTTGCGCGTTGGTT+GGG 0.274299 1.1:+34647950 MS.gene073610:CDS
GTTTGTTGCTGATAGGTTAT+TGG 0.303774 1.1:+34648005 MS.gene073610:CDS
GGGGCAAGAAGGGGTTCTTC+TGG 0.330658 1.1:-34647235 None:intergenic
GCGCGTTGGTTGGGATGAAT+GGG 0.334497 1.1:+34647959 MS.gene073610:CDS
ATGCAGAATCGGTCTGGGTT+TGG 0.338926 1.1:-34647256 None:intergenic
AAGCTGATTGGGCGATGAAA+TGG 0.368850 1.1:+34647650 MS.gene073610:CDS
GCGCTTCCTTGCGCGTTGGT+TGG 0.370769 1.1:+34647949 MS.gene073610:CDS
TTTGTTGCTGATAGGTTATT+GGG 0.379609 1.1:+34648006 MS.gene073610:CDS
ATGTTCAGAATCCGTTTGAT+TGG 0.390491 1.1:+34648055 MS.gene073610:CDS
ACCGTAGAAGGCACGTGCTT+CGG 0.402800 1.1:-34647500 None:intergenic
GTCTGGGTTTGGGGCAAGAA+GGG 0.415976 1.1:-34647245 None:intergenic
GGGAACATGCAGAATCGGTC+TGG 0.418807 1.1:-34647262 None:intergenic
GATTTCTTTCATGAATCTAC+CGG 0.435670 1.1:-34647470 None:intergenic
GTTGCATTTGCTTGTGTTGA+AGG 0.436600 1.1:+34647706 MS.gene073610:CDS
GAGCTTATTTCACGCGATGA+AGG 0.439919 1.1:+34647808 MS.gene073610:CDS
TCTGTTATGAATAGCTTGAA+TGG 0.443380 1.1:+34648147 MS.gene073610:CDS
TCAGAATCCGTTTGATTGGA+TGG 0.443597 1.1:+34648059 MS.gene073610:CDS
TCTGGGTTTGGGGCAAGAAG+GGG 0.444554 1.1:-34647244 None:intergenic
AGGCACGTGCTTCGGAGACT+TGG 0.444676 1.1:-34647492 None:intergenic
GCCGTCAGAGGCGGCGAAGA+AGG 0.447235 1.1:-34647428 None:intergenic
TGCAATCTGGAAACCGTAGA+AGG 0.456220 1.1:-34647512 None:intergenic
GAAAATCGCACAGAAGCTTC+CGG 0.456616 1.1:-34647740 None:intergenic
TCAGCTTTCTTCGCGATACA+TGG 0.459296 1.1:-34647634 None:intergenic
AATCGCTTATATACGTTTCG+AGG 0.468396 1.1:-34647570 None:intergenic
ATGGAATTGATTTCGCTTCA+AGG 0.479094 1.1:+34648078 MS.gene073610:CDS
ATCAATTCCATCCAATCAAA+CGG 0.480364 1.1:-34648066 None:intergenic
TCCGAAGCACGTGCCTTCTA+CGG 0.485174 1.1:+34647499 MS.gene073610:CDS
GATAGGTTATTGGGTGAGCT+TGG 0.486660 1.1:+34648015 MS.gene073610:CDS
TGAAATAAGCTCGTTTGAGA+AGG 0.487505 1.1:-34647797 None:intergenic
GGAACATGCAGAATCGGTCT+GGG 0.489835 1.1:-34647261 None:intergenic
ATATTGAGTTTGTTGCTGAT+AGG 0.493329 1.1:+34647998 MS.gene073610:CDS
GCCTTCTTCGCCGCCTCTGA+CGG 0.496560 1.1:+34647427 MS.gene073610:CDS
CGCGTTGGTTGGGATGAATG+GGG 0.498034 1.1:+34647960 MS.gene073610:CDS
GGAGGAAAGATCGACTTCTT+CGG 0.499480 1.1:-34647344 None:intergenic
GTGATGCTGTTGAAATTGAA+AGG 0.499878 1.1:+34647911 MS.gene073610:CDS
TATCGCGAAGAAAGCTGATT+GGG 0.501737 1.1:+34647639 MS.gene073610:CDS
AGGGGTTCTTCTGGAAAAGC+AGG 0.505225 1.1:-34647226 None:intergenic
GGGTGAGCTTGGTTGTGAAA+AGG 0.505349 1.1:+34648026 MS.gene073610:CDS
GGCGGTCCAGAATGAAGCTT+CGG 0.505885 1.1:-34647323 None:intergenic
GGTCTGGGTTTGGGGCAAGA+AGG 0.509820 1.1:-34647246 None:intergenic
TGGAATTGATTTCGCTTCAA+GGG 0.520571 1.1:+34648079 MS.gene073610:CDS
GTATCGCGAAGAAAGCTGAT+TGG 0.521675 1.1:+34647638 MS.gene073610:CDS
GGATTGGGAACATGCAGAAT+CGG 0.521690 1.1:-34647267 None:intergenic
GCAGAATCGGTCTGGGTTTG+GGG 0.524980 1.1:-34647254 None:intergenic
TGATGCTGTTGAAATTGAAA+GGG 0.545948 1.1:+34647912 MS.gene073610:CDS
ATGAATAGCTTGAATGGTAA+TGG 0.546534 1.1:+34648153 MS.gene073610:CDS
TTTCCTCCGATCTCAAGCAC+TGG 0.566756 1.1:+34647359 MS.gene073610:CDS
CGAGGACGTGAGAGATGAAG+TGG 0.584744 1.1:-34647405 None:intergenic
TTCATCCCAACCAACGCGCA+AGG 0.586445 1.1:-34647955 None:intergenic
TCGTTTGAGAAGGTTAATCC+CGG 0.587906 1.1:-34647787 None:intergenic
CAGAGGCGGCGAAGAAGGCG+AGG 0.590500 1.1:-34647423 None:intergenic
GCGTTGGTTGGGATGAATGG+GGG 0.596821 1.1:+34647961 MS.gene073610:CDS
GATGAAGTGGCGTTCGCCGT+CGG 0.597991 1.1:-34647392 None:intergenic
TTGCCAGTGCTTGAGATCGG+AGG 0.610798 1.1:-34647362 None:intergenic
TTCGAGGACGATGCCGTCAG+AGG 0.611655 1.1:-34647440 None:intergenic
GAGGACGATGCCGTCAGAGG+CGG 0.612579 1.1:-34647437 None:intergenic
GCTAAGTTCAGAGCGCGTGA+AGG 0.615081 1.1:+34647879 MS.gene073610:CDS
TGACGCGCTTCCTTGCGCGT+TGG 0.621614 1.1:+34647945 MS.gene073610:CDS
CACTGGCAAAACCTCACCGA+CGG 0.624263 1.1:+34647376 MS.gene073610:CDS
AGTGGCGTTCGCCGTCGGTG+AGG 0.626000 1.1:-34647387 None:intergenic
ATCGTCCTCGAAAATCTCGC+CGG 0.639774 1.1:+34647451 MS.gene073610:CDS
GGAAAGATCGACTTCTTCGG+CGG 0.687608 1.1:-34647341 None:intergenic
GAAGAGAGTACATCTCAGAG+TGG 0.694344 1.1:-34647546 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AATCCAATACCCAAAAATCT+GGG + chr1.1:34647285-34647304 MS.gene073610:CDS 30.0%
ATCAATTCCATCCAATCAAA+CGG - chr1.1:34648069-34648088 None:intergenic 30.0%
ATGAATAGCTTGAATGGTAA+TGG + chr1.1:34648153-34648172 MS.gene073610:CDS 30.0%
GATTTCTTTCATGAATCTAC+CGG - chr1.1:34647473-34647492 None:intergenic 30.0%
TCTGTTATGAATAGCTTGAA+TGG + chr1.1:34648147-34648166 MS.gene073610:CDS 30.0%
TGATGCTGTTGAAATTGAAA+GGG + chr1.1:34647912-34647931 MS.gene073610:CDS 30.0%
TTTGTTGCTGATAGGTTATT+GGG + chr1.1:34648006-34648025 MS.gene073610:CDS 30.0%
! TGTACATTTCCCAGATTTTT+GGG - chr1.1:34647297-34647316 None:intergenic 30.0%
! TTGTACATTTCCCAGATTTT+TGG - chr1.1:34647298-34647317 None:intergenic 30.0%
!! ATATTGAGTTTGTTGCTGAT+AGG + chr1.1:34647998-34648017 MS.gene073610:CDS 30.0%
!!! AGATTTTTGGGTATTGGATT+GGG - chr1.1:34647285-34647304 None:intergenic 30.0%
!!! ATTTTCTGGTTGAAAAAACG+TGG + chr1.1:34647757-34647776 MS.gene073610:CDS 30.0%
AAAAAACGTGGTTTAATGCC+GGG + chr1.1:34647769-34647788 MS.gene073610:CDS 35.0%
AATCGCTTATATACGTTTCG+AGG - chr1.1:34647573-34647592 None:intergenic 35.0%
ATGTTCAGAATCCGTTTGAT+TGG + chr1.1:34648055-34648074 MS.gene073610:CDS 35.0%
CAATCCAATACCCAAAAATC+TGG + chr1.1:34647284-34647303 MS.gene073610:CDS 35.0%
GAAAAAACGTGGTTTAATGC+CGG + chr1.1:34647768-34647787 MS.gene073610:CDS 35.0%
GTGATGCTGTTGAAATTGAA+AGG + chr1.1:34647911-34647930 MS.gene073610:CDS 35.0%
TGAAATAAGCTCGTTTGAGA+AGG - chr1.1:34647800-34647819 None:intergenic 35.0%
! ATGGAATTGATTTCGCTTCA+AGG + chr1.1:34648078-34648097 MS.gene073610:CDS 35.0%
! GGATGTTTTCAATTGCAATC+TGG - chr1.1:34647528-34647547 None:intergenic 35.0%
! GTTGAAGGAATCTTTTTCTC+CGG + chr1.1:34647721-34647740 MS.gene073610:CDS 35.0%
! GTTTGTTGCTGATAGGTTAT+TGG + chr1.1:34648005-34648024 MS.gene073610:CDS 35.0%
! TGGAATTGATTTCGCTTCAA+GGG + chr1.1:34648079-34648098 MS.gene073610:CDS 35.0%
!!! CAGATTTTTGGGTATTGGAT+TGG - chr1.1:34647286-34647305 None:intergenic 35.0%
!!! TGCTGATTTTTGCTTCAAGT+TGG + chr1.1:34648179-34648198 MS.gene073610:CDS 35.0%
!!! TTTCCCAGATTTTTGGGTAT+TGG - chr1.1:34647291-34647310 None:intergenic 35.0%
AAAAAGCCGAAGCTTCATTC+TGG + chr1.1:34647317-34647336 MS.gene073610:CDS 40.0%
GTTGCATTTGCTTGTGTTGA+AGG + chr1.1:34647706-34647725 MS.gene073610:CDS 40.0%
TATCGCGAAGAAAGCTGATT+GGG + chr1.1:34647639-34647658 MS.gene073610:CDS 40.0%
TCAGAATCCGTTTGATTGGA+TGG + chr1.1:34648059-34648078 MS.gene073610:CDS 40.0%
TCGTTTGAGAAGGTTAATCC+CGG - chr1.1:34647790-34647809 None:intergenic 40.0%
!!! AACTTTTTTGAGAAGCGCGT+TGG + chr1.1:34648108-34648127 MS.gene073610:CDS 40.0%
!!! ACTTTTTTGAGAAGCGCGTT+GGG + chr1.1:34648109-34648128 MS.gene073610:CDS 40.0%
AAGCTGATTGGGCGATGAAA+TGG + chr1.1:34647650-34647669 MS.gene073610:CDS 45.0%
GAAAATCGCACAGAAGCTTC+CGG - chr1.1:34647743-34647762 None:intergenic 45.0%
GAGCTTATTTCACGCGATGA+AGG + chr1.1:34647808-34647827 MS.gene073610:CDS 45.0%
GATAGGTTATTGGGTGAGCT+TGG + chr1.1:34648015-34648034 MS.gene073610:CDS 45.0%
GGATTGGGAACATGCAGAAT+CGG - chr1.1:34647270-34647289 None:intergenic 45.0%
GTATCGCGAAGAAAGCTGAT+TGG + chr1.1:34647638-34647657 MS.gene073610:CDS 45.0%
TCAGCTTTCTTCGCGATACA+TGG - chr1.1:34647637-34647656 None:intergenic 45.0%
TGCAATCTGGAAACCGTAGA+AGG - chr1.1:34647515-34647534 None:intergenic 45.0%
! GAAGAGAGTACATCTCAGAG+TGG - chr1.1:34647549-34647568 None:intergenic 45.0%
! GAAGCTTCTGTGCGATTTTC+TGG + chr1.1:34647743-34647762 MS.gene073610:CDS 45.0%
! GGAGGAAAGATCGACTTCTT+CGG - chr1.1:34647347-34647366 None:intergenic 45.0%
! GTTTTGCCAGTGCTTGAGAT+CGG - chr1.1:34647368-34647387 None:intergenic 45.0%
!!! CTTTTTTGAGAAGCGCGTTG+GGG + chr1.1:34648110-34648129 MS.gene073610:CDS 45.0%
ATCGTCCTCGAAAATCTCGC+CGG + chr1.1:34647451-34647470 MS.gene073610:CDS 50.0%
ATGCAGAATCGGTCTGGGTT+TGG - chr1.1:34647259-34647278 None:intergenic 50.0%
GGAACATGCAGAATCGGTCT+GGG - chr1.1:34647264-34647283 None:intergenic 50.0%
GGGTGAGCTTGGTTGTGAAA+AGG + chr1.1:34648026-34648045 MS.gene073610:CDS 50.0%
TGCAGAATCGGTCTGGGTTT+GGG - chr1.1:34647258-34647277 None:intergenic 50.0%
TTTCCTCCGATCTCAAGCAC+TGG + chr1.1:34647359-34647378 MS.gene073610:CDS 50.0%
! AGGGGTTCTTCTGGAAAAGC+AGG - chr1.1:34647229-34647248 None:intergenic 50.0%
! ATCTACCGGCGAGATTTTCG+AGG - chr1.1:34647459-34647478 None:intergenic 50.0%
! GGAAAGATCGACTTCTTCGG+CGG - chr1.1:34647344-34647363 None:intergenic 50.0%
CACTGGCAAAACCTCACCGA+CGG + chr1.1:34647376-34647395 MS.gene073610:CDS 55.0%
CGAGGACGTGAGAGATGAAG+TGG - chr1.1:34647408-34647427 None:intergenic 55.0%
GCAGAATCGGTCTGGGTTTG+GGG - chr1.1:34647257-34647276 None:intergenic 55.0%
GCTAAGTTCAGAGCGCGTGA+AGG + chr1.1:34647879-34647898 MS.gene073610:CDS 55.0%
GGCGGTCCAGAATGAAGCTT+CGG - chr1.1:34647326-34647345 None:intergenic 55.0%
GGGAACATGCAGAATCGGTC+TGG - chr1.1:34647265-34647284 None:intergenic 55.0%
TCCGAAGCACGTGCCTTCTA+CGG + chr1.1:34647499-34647518 MS.gene073610:CDS 55.0%
TTCATCCCAACCAACGCGCA+AGG - chr1.1:34647958-34647977 None:intergenic 55.0%
TTGCCAGTGCTTGAGATCGG+AGG - chr1.1:34647365-34647384 None:intergenic 55.0%
! ACCGTAGAAGGCACGTGCTT+CGG - chr1.1:34647503-34647522 None:intergenic 55.0%
! GTCTGGGTTTGGGGCAAGAA+GGG - chr1.1:34647248-34647267 None:intergenic 55.0%
! TCTGGGTTTGGGGCAAGAAG+GGG - chr1.1:34647247-34647266 None:intergenic 55.0%
!! CGCGTTGGTTGGGATGAATG+GGG + chr1.1:34647960-34647979 MS.gene073610:CDS 55.0%
!! GCGCGTTGGTTGGGATGAAT+GGG + chr1.1:34647959-34647978 MS.gene073610:CDS 55.0%
!! GCGTTGGTTGGGATGAATGG+GGG + chr1.1:34647961-34647980 MS.gene073610:CDS 55.0%
!! TGCGCGTTGGTTGGGATGAA+TGG + chr1.1:34647958-34647977 MS.gene073610:CDS 55.0%
AGGCACGTGCTTCGGAGACT+TGG - chr1.1:34647495-34647514 None:intergenic 60.0%
GATGAAGTGGCGTTCGCCGT+CGG - chr1.1:34647395-34647414 None:intergenic 60.0%
GGGGCAAGAAGGGGTTCTTC+TGG - chr1.1:34647238-34647257 None:intergenic 60.0%
TTCGAGGACGATGCCGTCAG+AGG - chr1.1:34647443-34647462 None:intergenic 60.0%
! GGTCTGGGTTTGGGGCAAGA+AGG - chr1.1:34647249-34647268 None:intergenic 60.0%
!! CGCTTCCTTGCGCGTTGGTT+GGG + chr1.1:34647950-34647969 MS.gene073610:CDS 60.0%
GAGGACGATGCCGTCAGAGG+CGG - chr1.1:34647440-34647459 None:intergenic 65.0%
GCCTTCTTCGCCGCCTCTGA+CGG + chr1.1:34647427-34647446 MS.gene073610:CDS 65.0%
TGACGCGCTTCCTTGCGCGT+TGG + chr1.1:34647945-34647964 MS.gene073610:CDS 65.0%
! GCGCTTCCTTGCGCGTTGGT+TGG + chr1.1:34647949-34647968 MS.gene073610:CDS 65.0%
AGTGGCGTTCGCCGTCGGTG+AGG - chr1.1:34647390-34647409 None:intergenic 70.0%
CAGAGGCGGCGAAGAAGGCG+AGG - chr1.1:34647426-34647445 None:intergenic 70.0%
GCCGTCAGAGGCGGCGAAGA+AGG - chr1.1:34647431-34647450 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 34647223 34648215 34647223 ID=MS.gene073610
chr1.1 mRNA 34647223 34648215 34647223 ID=MS.gene073610.t1;Parent=MS.gene073610
chr1.1 exon 34647223 34648215 34647223 ID=MS.gene073610.t1.exon1;Parent=MS.gene073610.t1
chr1.1 CDS 34647223 34648215 34647223 ID=cds.MS.gene073610.t1;Parent=MS.gene073610.t1
Gene Sequence

>MS.gene073610

ATGCCTGCTTTTCCAGAAGAACCCCTTCTTGCCCCAAACCCAGACCGATTCTGCATGTTCCCAATCCAATACCCAAAAATCTGGGAAATGTACAAAAAAGCCGAAGCTTCATTCTGGACCGCCGAAGAAGTCGATCTTTCCTCCGATCTCAAGCACTGGCAAAACCTCACCGACGGCGAACGCCACTTCATCTCTCACGTCCTCGCCTTCTTCGCCGCCTCTGACGGCATCGTCCTCGAAAATCTCGCCGGTAGATTCATGAAAGAAATCCAAGTCTCCGAAGCACGTGCCTTCTACGGTTTCCAGATTGCAATTGAAAACATCCACTCTGAGATGTACTCTCTTCTCCTCGAAACGTATATAAGCGATTCTGCTGAAAAGAATCGCTTATTTCATGCAATTGAAACAATTCCATGTATCGCGAAGAAAGCTGATTGGGCGATGAAATGGATCGATTCAAGTGATTCTTTTGCAGAAAGAATCGTTGCATTTGCTTGTGTTGAAGGAATCTTTTTCTCCGGAAGCTTCTGTGCGATTTTCTGGTTGAAAAAACGTGGTTTAATGCCGGGATTAACCTTCTCAAACGAGCTTATTTCACGCGATGAAGGATTACACTGTGATTTCGCTTGTTTGTTATACTCACTTCTACGCAAGAAGCTAAGTTCAGAGCGCGTGAAGGAAATTGTTTGTGATGCTGTTGAAATTGAAAGGGAGTTTGTGTGTGACGCGCTTCCTTGCGCGTTGGTTGGGATGAATGGGGGTTTGATGAGTACGTATATTGAGTTTGTTGCTGATAGGTTATTGGGTGAGCTTGGTTGTGAAAAGGTTTACAATGTTCAGAATCCGTTTGATTGGATGGAATTGATTTCGCTTCAAGGGAAAACAAACTTTTTTGAGAAGCGCGTTGGGGAGTATCAGAAAGCTTCTGTTATGAATAGCTTGAATGGTAATGGTGCTGCTGATTTTTGCTTCAAGTTGGATGAAGATTTTTAG

Protein sequence

>MS.gene073610.t1

MPAFPEEPLLAPNPDRFCMFPIQYPKIWEMYKKAEASFWTAEEVDLSSDLKHWQNLTDGERHFISHVLAFFAASDGIVLENLAGRFMKEIQVSEARAFYGFQIAIENIHSEMYSLLLETYISDSAEKNRLFHAIETIPCIAKKADWAMKWIDSSDSFAERIVAFACVEGIFFSGSFCAIFWLKKRGLMPGLTFSNELISRDEGLHCDFACLLYSLLRKKLSSERVKEIVCDAVEIEREFVCDALPCALVGMNGGLMSTYIEFVADRLLGELGCEKVYNVQNPFDWMELISLQGKTNFFEKRVGEYQKASVMNSLNGNGAADFCFKLDEDF