AlfalfaGEDB | MS.gene09849

M. sativa cultivar XinJiangDaYe / MS.gene09849

KEGG: K11252
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0032991 GO:0032993 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:1901363
PFAM: PF00125.27

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene09849.t1	XP_020212665.1	98.6	138	2	0	1	138	1	138	3.10E-41	177.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene09849.t1	Q1SU99	99.3	138	1	0	1	138	1	138	2.2e-43	176.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene09849.t1	A0A151TUN5	98.6	138	2	0	1	138	1	138	2.3e-41	177.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049255	MS.gene09849	0.812578	3.95E-51	-1.69E-46
MS.gene049341	MS.gene09849	0.801105	1.05E-48	-1.69E-46
MS.gene050689	MS.gene09849	0.814459	1.52E-51	-1.69E-46
MS.gene051328	MS.gene09849	0.8144	1.57E-51	-1.69E-46
MS.gene056215	MS.gene09849	0.816299	5.94E-52	-1.69E-46
MS.gene058976	MS.gene09849	0.822632	2.14E-53	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene09849	MS.gene035396	PPI
MS.gene09849	MS.gene65249	PPI
MS.gene09849	MS.gene08465	PPI
MS.gene09849	MS.gene99525	PPI
MS.gene09849	MS.gene004127	PPI
MS.gene09849	MS.gene025426	PPI
MS.gene09849	MS.gene64653	PPI
MS.gene09849	MS.gene041623	PPI
MS.gene09849	MS.gene025035	PPI
MS.gene09849	MS.gene63143	PPI
MS.gene09849	MS.gene75219	PPI
MS.gene09849	MS.gene81051	PPI
MS.gene09849	MS.gene65007	PPI
MS.gene09849	MS.gene004126	PPI
MS.gene09849	MS.gene63953	PPI
MS.gene09849	MS.gene066300	PPI
MS.gene09849	MS.gene049824	PPI
MS.gene09849	MS.gene27326	PPI
MS.gene09849	MS.gene24295	PPI
MS.gene09849	MS.gene066301	PPI
MS.gene09849	MS.gene049822	PPI
MS.gene09849	MS.gene26950	PPI
MS.gene066348	MS.gene09849	PPI
MS.gene09849	MS.gene051008	PPI
MS.gene09849	MS.gene025425	PPI
MS.gene09849	MS.gene84498	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene09849.t1	MTR_7g099960	98.551	138	2	0	1	138	1	138	1.01e-93	267
MS.gene09849.t1	MTR_4g063240	85.135	148	11	3	1	138	1	147	1.24e-80	234
MS.gene09849.t1	MTR_1g090780	92.806	139	7	3	1	138	1	137	3.35e-78	228
MS.gene09849.t1	MTR_1g492910	92.806	139	7	3	1	138	1	137	3.38e-78	228
MS.gene09849.t1	MTR_4g063220	85.906	149	9	3	1	138	1	148	6.41e-77	224
MS.gene09849.t1	MTR_4g063990	85.906	149	9	3	1	138	1	148	6.41e-77	224
MS.gene09849.t1	MTR_4g063200	85.906	149	9	3	1	138	1	148	6.41e-77	224
MS.gene09849.t1	MTR_4g064020	85.906	149	9	3	1	138	1	148	6.41e-77	224
MS.gene09849.t1	MTR_4g071180	85.906	149	9	3	1	138	1	148	7.00e-76	222
MS.gene09849.t1	MTR_4g070240	85.906	149	9	3	1	138	1	148	7.00e-76	222
MS.gene09849.t1	MTR_4g088025	86.806	144	12	3	1	138	1	143	1.82e-74	218
MS.gene09849.t1	MTR_4g070245	84.564	149	11	3	1	138	1	148	3.47e-74	218
MS.gene09849.t1	MTR_4g063275	83.221	149	13	3	1	138	1	148	1.11e-73	216
MS.gene09849.t1	MTR_4g064983	84.828	145	10	3	1	134	1	144	2.78e-72	216
MS.gene09849.t1	MTR_1g068600	95.370	108	4	1	31	138	30	136	7.95e-65	194
MS.gene09849.t1	MTR_2g084480	60.674	89	35	0	49	137	136	224	3.00e-37	127
MS.gene09849.t1	MTR_8g069990	71.910	89	22	2	9	97	3	88	2.53e-35	117
MS.gene09849.t1	MTR_4g063270	90.196	51	5	0	76	126	1	51	4.22e-27	95.9
MS.gene09849.t1	MTR_7g114580	90.244	41	4	0	98	138	11	51	6.81e-20	77.4
MS.gene09849.t1	MTR_4g032777	39.744	78	47	0	45	122	33	110	7.10e-13	61.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene09849.t1	AT2G37470	89.130	138	14	1	1	137	1	138	3.78e-78	228
MS.gene09849.t1	AT3G53650	89.130	138	15	0	1	138	1	138	6.07e-77	224
MS.gene09849.t1	AT3G45980	82.119	151	13	3	1	138	1	150	4.28e-74	218
MS.gene09849.t1	AT1G07790	81.879	149	15	2	1	138	1	148	3.71e-73	215
MS.gene09849.t1	AT2G28720	81.579	152	13	3	1	138	1	151	8.00e-73	214
MS.gene09849.t1	AT5G59910	85.185	135	18	1	6	138	16	150	6.42e-71	209
MS.gene09849.t1	AT3G46030	87.597	129	14	1	12	138	17	145	2.76e-70	208
MS.gene09849.t1	AT5G02570	84.397	141	10	3	1	138	1	132	3.15e-67	199
MS.gene09849.t1	AT5G22880	81.119	143	18	2	5	138	3	145	5.85e-66	197
MS.gene09849.t1	AT3G09480	82.114	123	21	1	16	138	5	126	1.50e-63	190
MS.gene09849.t1	AT1G08170	60.000	85	34	0	51	135	151	235	3.45e-37	127

Find 34 sgRNAs with CRISPR-Local

Find 33 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATCAAGAGCTGGTGAATT+TGG	0.235894	7.1:+7311718	None:intergenic
CTCCGCCTTTGGTTTCTTCT+CGG	0.278156	7.1:+7312054	None:intergenic
GCTGTTAGATTGGTTCTTCC+TGG	0.314946	7.1:-7311785	MS.gene09849:CDS
GAGATCTTCTTCTCCGCCTT+TGG	0.325858	7.1:+7312043	None:intergenic
GCCTTTGGTTTCTTCTCGGC+TGG	0.347622	7.1:+7312058	None:intergenic
ATTGGTTCTTCCTGGAGAAT+TGG	0.353160	7.1:-7311777	MS.gene09849:CDS
CATCTTCAAGGTTCTGAAAC+AGG	0.359264	7.1:-7311954	MS.gene09849:CDS
AAACAGGTTCATCCTGATAT+TGG	0.374570	7.1:-7311938	MS.gene09849:CDS
GATTCAAACTGCTGTTAGAT+TGG	0.401031	7.1:-7311795	MS.gene09849:CDS
TGCTCAGGAATCGTCTCGTT+TGG	0.412474	7.1:-7311855	MS.gene09849:CDS
TTGCTTGAAACACCAATATC+AGG	0.417581	7.1:+7311926	None:intergenic
TGGTGTTTCAAGCAAGGCTA+TGG	0.418611	7.1:-7311918	MS.gene09849:CDS
TCGGCTGGAACTTTCTCTGC+GGG	0.455951	7.1:+7312073	None:intergenic
TGCTGTTTCTGAAGGGACTA+AGG	0.469671	7.1:-7311747	MS.gene09849:CDS
ATACAAGATATACATCTTCA+AGG	0.488494	7.1:-7311966	MS.gene09849:CDS
GCTAAACATGCTGTTTCTGA+AGG	0.504101	7.1:-7311755	MS.gene09849:CDS
TCTGCGGGCTTCTTCTCCGC+GGG	0.504996	7.1:+7312088	None:intergenic
GCATGTTTAGCCAATTCTCC+AGG	0.514866	7.1:+7311767	None:intergenic
TATCTTTGAGAAACTTGCTC+AGG	0.528928	7.1:-7311870	MS.gene09849:CDS
ATCTCCCTTGAAGTGATCGT+CGG	0.552358	7.1:+7311815	None:intergenic
CTCTGCGGGCTTCTTCTCCG+CGG	0.552987	7.1:+7312087	None:intergenic
GGTGTTTCAAGCAAGGCTAT+GGG	0.553844	7.1:-7311917	MS.gene09849:CDS
AGAAGCCGACGATCACTTCA+AGG	0.560392	7.1:-7311820	MS.gene09849:CDS
GAGAAGAAGATCTCAAAAGA+AGG	0.561490	7.1:-7312034	MS.gene09849:CDS
TGATATTGGTGTTTCAAGCA+AGG	0.568075	7.1:-7311924	MS.gene09849:CDS
AGGAATCGTCTCGTTTGGCT+AGG	0.569875	7.1:-7311850	MS.gene09849:CDS
AAAGCAACAAAATCAAGAGC+TGG	0.570887	7.1:+7311709	None:intergenic
CTAAACATGCTGTTTCTGAA+GGG	0.585084	7.1:-7311754	MS.gene09849:CDS
CTCGGCTGGAACTTTCTCTG+CGG	0.596510	7.1:+7312072	None:intergenic
AAAGGCAGAAAAGAAACCCG+CGG	0.639354	7.1:-7312104	MS.gene09849:CDS
ATCAACAATGGCTCCCACAA+AGG	0.640475	7.1:-7312122	None:intergenic
GAAGCCGACGATCACTTCAA+GGG	0.644853	7.1:-7311819	MS.gene09849:CDS
TCCAGCCGAGAAGAAACCAA+AGG	0.673169	7.1:-7312059	MS.gene09849:CDS
AGCCGAGAAGAAACCAAAGG+CGG	0.691581	7.1:-7312056	MS.gene09849:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	ATACAAGATATACATCTTCA+AGG	-	chr7.1:7311870-7311889	MS.gene09849:CDS	25.0%
	AAACAGGTTCATCCTGATAT+TGG	-	chr7.1:7311898-7311917	MS.gene09849:CDS	35.0%
	GAGAAGAAGATCTCAAAAGA+AGG	-	chr7.1:7311802-7311821	MS.gene09849:CDS	35.0%
	GATTCAAACTGCTGTTAGAT+TGG	-	chr7.1:7312041-7312060	MS.gene09849:CDS	35.0%
	TTGCTTGAAACACCAATATC+AGG	+	chr7.1:7311913-7311932	None:intergenic	35.0%
!	TATCTTTGAGAAACTTGCTC+AGG	-	chr7.1:7311966-7311985	MS.gene09849:CDS	35.0%
!	TGATATTGGTGTTTCAAGCA+AGG	-	chr7.1:7311912-7311931	MS.gene09849:CDS	35.0%
!!	CTAAACATGCTGTTTCTGAA+GGG	-	chr7.1:7312082-7312101	MS.gene09849:CDS	35.0%
!	ATTGGTTCTTCCTGGAGAAT+TGG	-	chr7.1:7312059-7312078	MS.gene09849:CDS	40.0%
!	GGTTTCTTTTCTGCCTTTGT+GGG	+	chr7.1:7311730-7311749	None:intergenic	40.0%
!!	CATCTTCAAGGTTCTGAAAC+AGG	-	chr7.1:7311882-7311901	MS.gene09849:CDS	40.0%
!!	GCTAAACATGCTGTTTCTGA+AGG	-	chr7.1:7312081-7312100	MS.gene09849:CDS	40.0%
	AAAGGCAGAAAAGAAACCCG+CGG	-	chr7.1:7311732-7311751	MS.gene09849:CDS	45.0%
	ATCTCCCTTGAAGTGATCGT+CGG	+	chr7.1:7312024-7312043	None:intergenic	45.0%
	GCATGTTTAGCCAATTCTCC+AGG	+	chr7.1:7312072-7312091	None:intergenic	45.0%
	GGTGTTTCAAGCAAGGCTAT+GGG	-	chr7.1:7311919-7311938	MS.gene09849:CDS	45.0%
	TGGTGTTTCAAGCAAGGCTA+TGG	-	chr7.1:7311918-7311937	MS.gene09849:CDS	45.0%
!	GCTGTTAGATTGGTTCTTCC+TGG	-	chr7.1:7312051-7312070	MS.gene09849:CDS	45.0%
!	GGGTTTCTTTTCTGCCTTTG+TGG	+	chr7.1:7311731-7311750	None:intergenic	45.0%
!!	TGCTGTTTCTGAAGGGACTA+AGG	-	chr7.1:7312089-7312108	MS.gene09849:CDS	45.0%
	AGAAGCCGACGATCACTTCA+AGG	-	chr7.1:7312016-7312035	MS.gene09849:CDS	50.0%
	AGCCGAGAAGAAACCAAAGG+CGG	-	chr7.1:7311780-7311799	MS.gene09849:CDS	50.0%
	AGGAATCGTCTCGTTTGGCT+AGG	-	chr7.1:7311986-7312005	MS.gene09849:CDS	50.0%
	GAAGCCGACGATCACTTCAA+GGG	-	chr7.1:7312017-7312036	MS.gene09849:CDS	50.0%
	GAGATCTTCTTCTCCGCCTT+TGG	+	chr7.1:7311796-7311815	None:intergenic	50.0%
	TCCAGCCGAGAAGAAACCAA+AGG	-	chr7.1:7311777-7311796	MS.gene09849:CDS	50.0%
	TGCTCAGGAATCGTCTCGTT+TGG	-	chr7.1:7311981-7312000	MS.gene09849:CDS	50.0%
!	CTCCGCCTTTGGTTTCTTCT+CGG	+	chr7.1:7311785-7311804	None:intergenic	50.0%
	CTCGGCTGGAACTTTCTCTG+CGG	+	chr7.1:7311767-7311786	None:intergenic	55.0%
	TCGGCTGGAACTTTCTCTGC+GGG	+	chr7.1:7311766-7311785	None:intergenic	55.0%
!	GCCTTTGGTTTCTTCTCGGC+TGG	+	chr7.1:7311781-7311800	None:intergenic	55.0%
	CTCTGCGGGCTTCTTCTCCG+CGG	+	chr7.1:7311752-7311771	None:intergenic	65.0%
	TCTGCGGGCTTCTTCTCCGC+GGG	+	chr7.1:7311751-7311770	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.1	gene	7311721	7312137	7311721	ID=MS.gene09849
chr7.1	mRNA	7311721	7312137	7311721	ID=MS.gene09849.t1;Parent=MS.gene09849
chr7.1	exon	7311721	7312137	7311721	ID=MS.gene09849.t1.exon1;Parent=MS.gene09849.t1
chr7.1	CDS	7311721	7312137	7311721	ID=cds.MS.gene09849.t1;Parent=MS.gene09849.t1

Gene Sequence

>MS.gene09849

ATGGCTCCCACAAAGGCAGAAAAGAAACCCGCGGAGAAGAAGCCCGCAGAGAAAGTTCCAGCCGAGAAGAAACCAAAGGCGGAGAAGAAGATCTCAAAAGAAGGAAGCAGCGACAAGAAGAAAAAGAGAACGAAGAAAAGCGTTGAAACATACAAGATATACATCTTCAAGGTTCTGAAACAGGTTCATCCTGATATTGGTGTTTCAAGCAAGGCTATGGGAATTATGAACAGTTTCATTAACGATATCTTTGAGAAACTTGCTCAGGAATCGTCTCGTTTGGCTAGGTATAACAAGAAGCCGACGATCACTTCAAGGGAGATTCAAACTGCTGTTAGATTGGTTCTTCCTGGAGAATTGGCTAAACATGCTGTTTCTGAAGGGACTAAGGCTGTTACCAAATTCACCAGCTCTTGA

Protein sequence

>MS.gene09849.t1

MAPTKAEKKPAEKKPAEKVPAEKKPKAEKKISKEGSSDKKKKRTKKSVETYKIYIFKVLKQVHPDIGVSSKAMGIMNSFINDIFEKLAQESSRLARYNKKPTITSREIQTAVRLVLPGELAKHAVSEGTKAVTKFTSS