Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20584.t1 | XP_003618369.1 | 95.8 | 95 | 4 | 0 | 1 | 95 | 1 | 95 | 1.70E-46 | 194.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20584.t1 | G7KJF9 | 95.8 | 95 | 4 | 0 | 1 | 95 | 1 | 95 | 1.2e-46 | 194.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050303 | MS.gene20584 | 0.813495 | 2.49E-51 | -1.69E-46 |
| MS.gene050330 | MS.gene20584 | -0.801618 | 8.27E-49 | -1.69E-46 |
| MS.gene051983 | MS.gene20584 | 0.802761 | 4.81E-49 | -1.69E-46 |
| MS.gene052176 | MS.gene20584 | 0.82624 | 3.02E-54 | -1.69E-46 |
| MS.gene052309 | MS.gene20584 | -0.878689 | 2.29E-69 | -1.69E-46 |
| MS.gene052358 | MS.gene20584 | -0.825297 | 5.06E-54 | -1.69E-46 |
| MS.gene052376 | MS.gene20584 | 0.800438 | 1.44E-48 | -1.69E-46 |
| MS.gene053188 | MS.gene20584 | 0.852648 | 4.02E-61 | -1.69E-46 |
| MS.gene053216 | MS.gene20584 | 0.832432 | 9.49E-56 | -1.69E-46 |
| MS.gene053294 | MS.gene20584 | 0.820448 | 6.82E-53 | -1.69E-46 |
| MS.gene054537 | MS.gene20584 | 0.864682 | 1.02E-64 | -1.69E-46 |
| MS.gene056087 | MS.gene20584 | 0.842255 | 2.89E-58 | -1.69E-46 |
| MS.gene057060 | MS.gene20584 | 0.820637 | 6.17E-53 | -1.69E-46 |
| MS.gene057079 | MS.gene20584 | 0.848751 | 5.02E-60 | -1.69E-46 |
| MS.gene05795 | MS.gene20584 | 0.822641 | 2.13E-53 | -1.69E-46 |
| MS.gene058200 | MS.gene20584 | 0.817431 | 3.31E-52 | -1.69E-46 |
| MS.gene058498 | MS.gene20584 | 0.801471 | 8.86E-49 | -1.69E-46 |
| MS.gene060297 | MS.gene20584 | 0.819284 | 1.26E-52 | -1.69E-46 |
| MS.gene061040 | MS.gene20584 | -0.811381 | 7.20E-51 | -1.69E-46 |
| MS.gene061114 | MS.gene20584 | -0.800215 | 1.60E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20584.t1 | MTR_6g008680 | 95.789 | 95 | 4 | 0 | 1 | 95 | 1 | 95 | 6.91e-65 | 191 |
| MS.gene20584.t1 | MTR_2g105430 | 43.103 | 58 | 31 | 1 | 10 | 65 | 68 | 125 | 6.38e-13 | 60.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20584.t1 | AT5G43150 | 46.667 | 60 | 32 | 0 | 8 | 67 | 5 | 64 | 2.63e-14 | 62.8 |
Find 17 sgRNAs with CRISPR-Local
Find 61 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGACTTCACATCATTTCTT+AGG | 0.245992 | 6.3:-4546697 | None:intergenic |
| ATTTCCATTGCCACACGTTT+TGG | 0.291787 | 6.3:+4545586 | MS.gene20584:CDS |
| GAAATCCAAAACCTTCTAAA+AGG | 0.302858 | 6.3:-4545568 | None:intergenic |
| AATGAATCATTATCAGAGTT+TGG | 0.349611 | 6.3:+4546764 | MS.gene20584:CDS |
| AGTACTTGAAATTATTCAAA+TGG | 0.355186 | 6.3:+4546822 | MS.gene20584:CDS |
| GTACTTGAAATTATTCAAAT+GGG | 0.452163 | 6.3:+4546823 | MS.gene20584:CDS |
| GAAGATGAAATTCTCTCGGC+AGG | 0.497923 | 6.3:+4545519 | MS.gene20584:CDS |
| TGAAATTATTCAAATGGGCA+AGG | 0.527275 | 6.3:+4546828 | MS.gene20584:CDS |
| AATTCCAAAACGTGTGGCAA+TGG | 0.529168 | 6.3:-4545590 | None:intergenic |
| TCATTATCAGAGTTTGGTGG+TGG | 0.560367 | 6.3:+4546770 | MS.gene20584:CDS |
| ATGAAGATATTCGTGTGATG+TGG | 0.570449 | 6.3:+4546726 | MS.gene20584:CDS |
| TGGAGAAGATGAAATTCTCT+CGG | 0.589361 | 6.3:+4545515 | MS.gene20584:CDS |
| TCTCTCGGCAGGTGGAGTTG+TGG | 0.592580 | 6.3:+4545530 | MS.gene20584:CDS |
| TGAAGATATTCGTGTGATGT+GGG | 0.602380 | 6.3:+4546727 | MS.gene20584:CDS |
| GATGAAATTCTCTCGGCAGG+TGG | 0.607611 | 6.3:+4545522 | MS.gene20584:CDS |
| TTTACGAATTCCAAAACGTG+TGG | 0.611706 | 6.3:-4545596 | None:intergenic |
| GAATCATTATCAGAGTTTGG+TGG | 0.650506 | 6.3:+4546767 | MS.gene20584:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TAAAAAAGAAAAAGATTTTA+AGG | - | chr6.3:4546213-4546232 | None:intergenic | 10.0% |
| !! | AGTACTTGAAATTATTCAAA+TGG | + | chr6.3:4546822-4546841 | MS.gene20584:CDS | 20.0% |
| !! | ATCAAATCACTCAAAATATT+TGG | - | chr6.3:4546108-4546127 | None:intergenic | 20.0% |
| !! | GTAATAATTCAAAACATGTT+TGG | + | chr6.3:4545623-4545642 | MS.gene20584:intron | 20.0% |
| !! | GTACTTGAAATTATTCAAAT+GGG | + | chr6.3:4546823-4546842 | MS.gene20584:CDS | 20.0% |
| !! | GTGAATTATTGTAACATATT+TGG | + | chr6.3:4545791-4545810 | MS.gene20584:intron | 20.0% |
| !! | TTGAAATAACCTTGTTAATT+TGG | + | chr6.3:4545945-4545964 | MS.gene20584:intron | 20.0% |
| !! | TTGGAATAAACATGTTAATT+TGG | + | chr6.3:4546590-4546609 | MS.gene20584:intron | 20.0% |
| !!! | AATCTTTTTCTTTTTTATGC+CGG | + | chr6.3:4546216-4546235 | MS.gene20584:intron | 20.0% |
| !!! | TCACATTGAATATTCTTTTT+AGG | + | chr6.3:4546141-4546160 | MS.gene20584:intron | 20.0% |
| !!! | TCATCAAAATCAAGATTTTA+AGG | - | chr6.3:4546336-4546355 | None:intergenic | 20.0% |
| !!! | TCTTCAAAATCAAGATTTTA+AGG | - | chr6.3:4546284-4546303 | None:intergenic | 20.0% |
| !!! | TTGATTTTGATGATTATTTG+TGG | + | chr6.3:4546343-4546362 | MS.gene20584:intron | 20.0% |
| !!! | TTTTATCAAAATAGTGATTC+TGG | + | chr6.3:4545856-4545875 | MS.gene20584:intron | 20.0% |
| ! | AAACAATGAGTTACTGAAAT+TGG | + | chr6.3:4546652-4546671 | MS.gene20584:intron | 25.0% |
| ! | AAGGATTTGATTCTTATACA+CGG | - | chr6.3:4546242-4546261 | None:intergenic | 25.0% |
| ! | AATGAATCATTATCAGAGTT+TGG | + | chr6.3:4546764-4546783 | MS.gene20584:CDS | 25.0% |
| ! | ATTTCTTGTCCAAATTAACA+AGG | - | chr6.3:4545957-4545976 | None:intergenic | 25.0% |
| ! | CTGAATCAATAATCTTCTAA+AGG | - | chr6.3:4546261-4546280 | None:intergenic | 25.0% |
| ! | TCTTCAAAAATCAGATAGAA+CGG | - | chr6.3:4545984-4546003 | None:intergenic | 25.0% |
| ! | TGAATTGACAATGAATTTAG+CGG | - | chr6.3:4546523-4546542 | None:intergenic | 25.0% |
| ! | TTGGTTATAAAGTACATGTT+TGG | + | chr6.3:4545663-4545682 | MS.gene20584:intron | 25.0% |
| !! | TGAATTTTCTTGATTTTGCT+TGG | - | chr6.3:4546803-4546822 | None:intergenic | 25.0% |
| !!! | ATTGAATATTCTTTTTAGGC+CGG | + | chr6.3:4546145-4546164 | MS.gene20584:intron | 25.0% |
| !!! | GGTCTTTTTTCTGTATTTTT+TGG | + | chr6.3:4545644-4545663 | MS.gene20584:intron | 25.0% |
| !!! | TTCTATCTGATTTTTGAAGA+TGG | + | chr6.3:4545984-4546003 | MS.gene20584:intron | 25.0% |
| AAGACTTCACATCATTTCTT+AGG | - | chr6.3:4546700-4546719 | None:intergenic | 30.0% | |
| ACATAATCAGAGAGCTATAT+AGG | - | chr6.3:4546629-4546648 | None:intergenic | 30.0% | |
| ATTTGATACTTATACACTGC+CGG | - | chr6.3:4546167-4546186 | None:intergenic | 30.0% | |
| CACGTTAATTGTAACATGTT+TGG | + | chr6.3:4545733-4545752 | MS.gene20584:intron | 30.0% | |
| CTGAATCAATAATCTTCAAC+AGG | - | chr6.3:4546190-4546209 | None:intergenic | 30.0% | |
| GAAATCCAAAACCTTCTAAA+AGG | - | chr6.3:4545571-4545590 | None:intergenic | 30.0% | |
| TGAAATTATTCAAATGGGCA+AGG | + | chr6.3:4546828-4546847 | MS.gene20584:CDS | 30.0% | |
| TGAAATTGGAAAAATGTGCA+GGG | + | chr6.3:4546666-4546685 | MS.gene20584:intron | 30.0% | |
| TGGTGAGTTTGATGAAATTA+TGG | + | chr6.3:4546431-4546450 | MS.gene20584:intron | 30.0% | |
| ! | ATATCATGCATCCTTTTAGA+AGG | + | chr6.3:4545557-4545576 | MS.gene20584:CDS | 30.0% |
| ! | TTGTAACATGTTTGGAATCA+CGG | + | chr6.3:4545741-4545760 | MS.gene20584:intron | 30.0% |
| ACTTGAGTGAATGCAAAGTT+TGG | + | chr6.3:4546571-4546590 | MS.gene20584:intron | 35.0% | |
| AGAACCACGACTAATTACAT+GGG | + | chr6.3:4546055-4546074 | MS.gene20584:intron | 35.0% | |
| ATGAAGATATTCGTGTGATG+TGG | + | chr6.3:4546726-4546745 | MS.gene20584:CDS | 35.0% | |
| ATTTGATTCTTATACACGGC+CGG | - | chr6.3:4546238-4546257 | None:intergenic | 35.0% | |
| CAATGAATTTAGCGGAATCA+CGG | - | chr6.3:4546515-4546534 | None:intergenic | 35.0% | |
| CTGAAATTGGAAAAATGTGC+AGG | + | chr6.3:4546665-4546684 | MS.gene20584:intron | 35.0% | |
| GAATCATTATCAGAGTTTGG+TGG | + | chr6.3:4546767-4546786 | MS.gene20584:CDS | 35.0% | |
| TATAGCTTCTGCTAAAATCG+TGG | + | chr6.3:4546483-4546502 | MS.gene20584:intron | 35.0% | |
| TGAAGATATTCGTGTGATGT+GGG | + | chr6.3:4546727-4546746 | MS.gene20584:CDS | 35.0% | |
| TGGAGAAGATGAAATTCTCT+CGG | + | chr6.3:4545515-4545534 | MS.gene20584:CDS | 35.0% | |
| TTTACGAATTCCAAAACGTG+TGG | - | chr6.3:4545599-4545618 | None:intergenic | 35.0% | |
| !! | TGCATCCTTTTAGAAGGTTT+TGG | + | chr6.3:4545563-4545582 | MS.gene20584:CDS | 35.0% |
| !!! | CGTTTTGGAATTCGTAAAAC+TGG | + | chr6.3:4545601-4545620 | MS.gene20584:CDS | 35.0% |
| AATTCCAAAACGTGTGGCAA+TGG | - | chr6.3:4545593-4545612 | None:intergenic | 40.0% | |
| GAGAACCACGACTAATTACA+TGG | + | chr6.3:4546054-4546073 | MS.gene20584:intron | 40.0% | |
| TTGTAGCGTGTTTGAATTGC+TGG | + | chr6.3:4546411-4546430 | MS.gene20584:intron | 40.0% | |
| ! | ATTTCCATTGCCACACGTTT+TGG | + | chr6.3:4545586-4545605 | MS.gene20584:CDS | 40.0% |
| ! | TCATTATCAGAGTTTGGTGG+TGG | + | chr6.3:4546770-4546789 | MS.gene20584:CDS | 40.0% |
| ACCACGACTAATTACATGGG+TGG | + | chr6.3:4546058-4546077 | MS.gene20584:intron | 45.0% | |
| GAAGATGAAATTCTCTCGGC+AGG | + | chr6.3:4545519-4545538 | MS.gene20584:CDS | 45.0% | |
| !! | GGTGGCTTTAGAAGTAGTTG+TGG | + | chr6.3:4546076-4546095 | MS.gene20584:intron | 45.0% |
| GATGAAATTCTCTCGGCAGG+TGG | + | chr6.3:4545522-4545541 | MS.gene20584:CDS | 50.0% | |
| GCCACCCATGTAATTAGTCG+TGG | - | chr6.3:4546062-4546081 | None:intergenic | 50.0% | |
| TCTCTCGGCAGGTGGAGTTG+TGG | + | chr6.3:4545530-4545549 | MS.gene20584:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.3 | gene | 4545514 | 4546865 | 4545514 | ID=MS.gene20584 |
| chr6.3 | mRNA | 4545514 | 4546865 | 4545514 | ID=MS.gene20584.t1;Parent=MS.gene20584 |
| chr6.3 | exon | 4545514 | 4545622 | 4545514 | ID=MS.gene20584.t1.exon1;Parent=MS.gene20584.t1 |
| chr6.3 | CDS | 4545514 | 4545622 | 4545514 | ID=cds.MS.gene20584.t1;Parent=MS.gene20584.t1 |
| chr6.3 | exon | 4546687 | 4546865 | 4546687 | ID=MS.gene20584.t1.exon2;Parent=MS.gene20584.t1 |
| chr6.3 | CDS | 4546687 | 4546865 | 4546687 | ID=cds.MS.gene20584.t1;Parent=MS.gene20584.t1 |
| Gene Sequence |
| Protein sequence |
