AlfalfaGEDB | MS.gene22159

M. sativa cultivar XinJiangDaYe / MS.gene22159

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene22159.t1	PNX96947.1	92	377	25	4	36	409	41	415	3.20E-198	701

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene22159.t1	Q680C0	27.8	360	226	9	40	383	9	350	2.3e-35	151.4

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene22159.t1	A0A2K3N1N9	92.0	377	25	4	36	409	41	415	2.3e-198	701.0

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049989	MS.gene22159	0.866704	2.35E-65	-1.69E-46
MS.gene050297	MS.gene22159	0.839825	1.26E-57	-1.69E-46
MS.gene051742	MS.gene22159	0.806902	6.57E-50	-1.69E-46
MS.gene051955	MS.gene22159	0.803304	3.72E-49	-1.69E-46
MS.gene052308	MS.gene22159	0.80284	4.63E-49	-1.69E-46
MS.gene052367	MS.gene22159	0.856458	3.17E-62	-1.69E-46
MS.gene053173	MS.gene22159	0.830101	3.55E-55	-1.69E-46
MS.gene053416	MS.gene22159	0.847086	1.44E-59	-1.69E-46
MS.gene053417	MS.gene22159	0.845342	4.31E-59	-1.69E-46
MS.gene054551	MS.gene22159	0.822127	2.80E-53	-1.69E-46

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene22159.t1	MTR_3g046880	96.377	414	11	3	1	412	1	412	0.0	818
MS.gene22159.t1	MTR_5g091200	72.283	368	81	6	36	394	13	368	0.0	539
MS.gene22159.t1	MTR_5g091240	58.516	364	132	6	43	395	16	371	1.74e-133	403
MS.gene22159.t1	MTR_5g091240	42.623	183	77	7	218	394	514	674	9.37e-27	113
MS.gene22159.t1	MTR_5g091240	33.742	163	76	5	237	395	385	519	1.68e-12	69.7
MS.gene22159.t1	MTR_5g091230	54.777	314	118	8	103	406	7	306	3.66e-101	311
MS.gene22159.t1	MTR_8g099555	33.518	361	201	8	39	383	7	344	9.93e-59	195
MS.gene22159.t1	MTR_5g028370	30.495	364	202	7	39	380	7	341	4.89e-48	167
MS.gene22159.t1	MTR_5g028370	30.495	364	202	7	39	380	19	353	6.89e-48	167
MS.gene22159.t1	MTR_5g009140	30.278	360	190	11	40	380	8	325	2.77e-36	135

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene22159.t1	AT5G50890	69.251	374	103	5	30	394	36	406	0.0	532
MS.gene22159.t1	AT2G05260	29.973	367	218	10	40	383	8	358	2.57e-48	168
MS.gene22159.t1	AT5G24230	30.504	377	215	9	17	369	1	354	7.17e-48	167
MS.gene22159.t1	AT5G24230	30.504	377	215	9	17	369	1	354	7.17e-48	167
MS.gene22159.t1	AT5G24230	30.504	377	215	9	17	369	1	354	7.17e-48	167
MS.gene22159.t1	AT4G10955	28.056	360	225	9	40	383	9	350	1.19e-45	161
MS.gene22159.t1	AT4G10955	28.056	360	225	9	40	383	9	350	1.19e-45	161
MS.gene22159.t1	AT2G05260	31.884	276	172	5	40	315	8	267	1.26e-44	157
MS.gene22159.t1	AT5G24220	27.855	359	212	8	39	377	24	355	1.66e-42	153
MS.gene22159.t1	AT5G24180	28.418	373	213	10	29	377	12	354	3.21e-40	147

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Find 71 sgRNAs with CRISPR-Local

Find 215 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGAACTGGAAGAGGAAA+AGG	0.409815	3.4:+41831723	MS.gene22159:CDS
AAAATGCTCTAGCTCCAAAT+TGG	0.383483	3.4:+41833912	MS.gene22159:CDS
AAAGCCTAAAAGGTTCTGTA+AGG	0.545305	3.4:+41834158	MS.gene22159:CDS
AAAGGTGTTTGTTGTGAATA+AGG	0.307684	3.4:+41834678	MS.gene22159:CDS
AAATCAGTGTCTGATGCATA+TGG	0.439991	3.4:+41834205	MS.gene22159:CDS
AAGCTCATGGAATTGAACAA+TGG	0.723699	3.4:+41834719	MS.gene22159:CDS
AATGTCGGTCCAAATAATAA+TGG	0.479362	3.4:+41834640	MS.gene22159:CDS
AATTCTTCTCAAGTATTGCA+GGG	0.565859	3.4:+41834838	MS.gene22159:CDS
ACAAGAAGAGTTGGATGCCA+TGG	0.575559	3.4:+41834470	MS.gene22159:CDS
ACACTTATAGACGAAAGAGA+CGG	0.699729	3.4:+41833965	MS.gene22159:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AATGTCGGTCCAAATAATAA+TGG	+	chr3.4:41834640-41834659	MS.gene22159:CDS	30.0%
AGATCAAATGAGAAAGATGA+TGG	+	chr3.4:41834598-41834617	MS.gene22159:CDS	30.0%
AGCCTATTCTAGATAATTTC+GGG	-	chr3.4:41832822-41832841	None:intergenic	30.0%
CAGAGTCACATATATTACTA+AGG	-	chr3.4:41832972-41832991	None:intergenic	30.0%
CAGCTTAAATCTTTGTATAC+AGG	+	chr3.4:41834799-41834818	MS.gene22159:CDS	30.0%
CTTAAATCTTTGTATACAGG+TGG	+	chr3.4:41834802-41834821	MS.gene22159:CDS	30.0%
GATAATAACAAGGAGAATGT+CGG	+	chr3.4:41834625-41834644	MS.gene22159:CDS	30.0%
TAGTAGTAACACCATACTAT+CGG	-	chr3.4:41833398-41833417	None:intergenic	30.0%
TCTAATCAACCAGTTAAAAC+AGG	-	chr3.4:41833017-41833036	None:intergenic	30.0%
TCTTTCTCATTTGATCTATC+AGG	-	chr3.4:41834595-41834614	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr3.4	gene	41831682	41834876	41831682	ID=MS.gene22159
chr3.4	mRNA	41831682	41834876	41831682	ID=MS.gene22159.t1;Parent=MS.gene22159
chr3.4	exon	41831682	41831863	41831682	ID=MS.gene22159.t1.exon1;Parent=MS.gene22159.t1
chr3.4	CDS	41831682	41831863	41831682	ID=cds.MS.gene22159.t1;Parent=MS.gene22159.t1
chr3.4	exon	41833820	41834876	41833820	ID=MS.gene22159.t1.exon2;Parent=MS.gene22159.t1
chr3.4	CDS	41833820	41834876	41833820	ID=cds.MS.gene22159.t1;Parent=MS.gene22159.t1

Gene Sequence

>MS.gene22159

ATGAACACGAACATGGAAATCACTACAAAGGAAGAAGAAGTAAAAGAACTGGAAGAGGAAAAGGTTGTTGTGGTTGAAAAGCAAGGAGAAGAAGGAGAAGCTCATCATCCTTATGCATTTCACGTGTCTGGTCCAAGGAATTTGGTTAATCTTAATTGGAGAGATCTCATTAGTTCTAGCTGGTGAGTACCCTTTTTATTTGTAGCTTTTGTTTGATTTGATTTGATGAAAATTTTGGTTTTTGTTATGTGGGTTTGTTTCTATTTTTTGAAATTTTGAGTGAATTTAAAATTTGGTTTTATTTGAAATGGATGAACAGAGTGTGAATTATCTTTTGGTTTGGTACTTTTTTGAAGGGTTTAGGGTTTGGTGAAAATTTGAGAAAATGGGTTTTGAGAATTTAAGTTAGTTTCTAAATGGGTTTGTTTAGAATTTTGAATTTAAATTTAGGGTTCATTCACTTTATAACGTTTTTAGTTTGATCCGTTTTCAGAAAATATTGGGTTTTCAGATTTGAGGTTTTTGATTGACGGAACTATGGATTTTTAGGTTTGAGGTTTTAAGATTTAAGTTTATGACCTATGAAGCACGGATACACCGGACACGACACGGACACAGACATGTCTACACCGCTAATAATTTGAGTAAATAACATTATTCAGTGTAATTTTATTTGTCTGTGTCATGTTGGTGTCAGACACATGTTGGTGTCAGACACGCCTAATCTGAGTAGTGTTCGTGCTTCATAGTTTATGATAGATAATTTTTTGATGAGTTTCTGGAATCTTTGTGATTTAAACTATTTTTTAGATAATAGCTTCATTAATTTAACTACAGGCTTTGGATAATATATTCTGATTCCCAATTTATTTATTTCCTTAATTGTTTTGTAATTGGGATCGAAATTTGGGGATCCTGATTTGGAGCATTGGCCATTAATATCATGGATTTTCTTAATTCCCTGTTTAGTTTTGATATGAATTTTTCTGCATTGTGTTTGCAAAATTGTCGCCTCTGACTTTGGCTCTCAATTGATTTTTTTAAATTGAGATAAAGGACATTAGAATTTCATGAACTTGTTTTTCTTTATTTTATTATTAGTAATAGAGCTTTGCTATTTCTAGCGAAGCAATTTGTCCCGAAATTATCTAGAATAGGCTATCACCGTTAGATTGGAAACTGCCACAATACATGGGTGGGCGGGCATACAAAGTGGATTGGGCTCCACTTAATTTAACACGGTCGGGATACTGTCGTGAATTTTTTTCTCGGGAGAAATAGCTTTTTCCTTAGTAATATATGTGACTCTGACTTAAATCAATGATATAAAAACCTGTTTTAACTGGTTGATTAGACCTATGAACACGGACACAAACCAGACACGATACTAACACGTCGACATGGAAAATAATTTAAGAAAATAGATATGAGGGCACATAACTACATATGCTGTTGTCGAGTCAATGCTACCTAGGATTAGTAGTTAGACCAGAAATCGGTCAGAGTCACCATGTCGTTCTGAAATGCATCTGAACCTGTGGAAACGAGTTTGATTTTCCTGGTTAAATCAATGAAACCAGTTGAACCAGCTTTCAATTATTTAAGTTTTGCATCAATTTTATACATTTGTAATTTATTGGTTCGACCAGTGACTCGTTCAGTCAATAATCAATAGCATAATTGAGTTGATTATGGATATGAGTTTAACATTGTCCGATAGTATGGTGTTACTACTAACTCCAGCCAGAGTCCCCTATTTTTCATATGCTTCCTTCTACTTTGTGCAGTTGCGTTAGTATTGTGTTGGTGTCGGACACTAACACACATCAAACACGCTTCAATCTGAAGTGTGAAGGAAACATAGAAGGATGCAAATTTTACAATTATTTCAGTTTGCATCACCTTTATACAATTGTAACTTATTGGTTTGACTAGTGATCATCTATTGATTTAATACCTTATTTGACTGTATTATCAGTATGGGTTTTATAATATTGTTTGAGAGAGGACCATGTCTCTAATATTTTCAGCAAGAGGTCCCTAGTTTTTCATTTTCGTTCTTGTTGAACCTGATTGAGAATCCTCCAACCTTTACTAGAACTTCATATAATTACTGATACCAATTTTGTTGCATGCAGGAAGGATCCAAACTACAAGAGAACTGTCATTGCTTGTTTTATACAGGCAGTGTACTTGCTTGAACTTGATCGACAAGAGAAACGAACACCAGAAAATGCTCTAGCTCCAAATTGGTGGATTCCCTTCAAGTACAAGCTAACAAAAACACTTATAGACGAAAGAGACGGTTCAATTTTCGGTGCAATACTCGAATGGGACAGATCTGCAGCGATGTCCGATTTAGTACTGATCAGACCAAGTGGTGCACCAAAAGCCGTTTTAGCACTAAGAGGAACATTACTCAAAAGCCACACAATGCGAAGAGACATCCAAGATGACCTTCGGTTCCTCGCTTGGGAAAGCCTAAAAGGTTCTGTAAGGTTCAATGTAGCTTTAGAGACACTAAAATCAGTGTCTGATGCATATGGAAGTAGCAATGTTTGTGTAGCAGGACATTCATTAGGAGCTGGTTTTGCACTTCAAGTTGGAAAATCGTTAGCAAAAGAAGGTATCTATGTAGAAACACATTTATTCAACCCGCCTTCTGTTTCAATTGCTATGAGTCTAAGAAACATTGGAGAAAAAGCCGAGTTAGCTTGGAAGAGAATCAAATCAATGCTTCCTTCAAGCAGCGAAGGAGTTGATAATATCAACAACGATGGAAACAACAACAAGAAGAGTTGGATGCCATGGTTAACTAGCTTGAAGAATCATAATTTTGGTGTTGGAAAATGGGTTCCTCATCTCTATGTGAACAATAGTGACTATATATGTTGTTCTTATAATGATGAACCTGATAGATCAAATGAGAAAGATGATGGTGGTGATAATAACAAGGAGAATGTCGGTCCAAATAATAATGGTTGTCATGTTGCTGCAAAGGTGTTTGTTGTGAATAAGGAAAAGCAGAAGTTTCATGAAGCTCATGGAATTGAACAATGGTGGTCTAATGATTCACAGCTTCAGCAGACTATTCATACTAGTAAACTCATAAGCAGACAGCTTAAATCTTTGTATACAGGTGGTAGTGGTAGTGGTAATTCTTCTCAAGTATTGCAGGGAAAGGCTAGTAAATAA

Protein sequence

>MS.gene22159.t1

MNTNMEITTKEEEVKELEEEKVVVVEKQGEEGEAHHPYAFHVSGPRNLVNLNWRDLISSSWKDPNYKRTVIACFIQAVYLLELDRQEKRTPENALAPNWWIPFKYKLTKTLIDERDGSIFGAILEWDRSAAMSDLVLIRPSGAPKAVLALRGTLLKSHTMRRDIQDDLRFLAWESLKGSVRFNVALETLKSVSDAYGSSNVCVAGHSLGAGFALQVGKSLAKEGIYVETHLFNPPSVSIAMSLRNIGEKAELAWKRIKSMLPSSSEGVDNINNDGNNNKKSWMPWLTSLKNHNFGVGKWVPHLYVNNSDYICCSYNDEPDRSNEKDDGGDNNKENVGPNNNGCHVAAKVFVVNKEKQKFHEAHGIEQWWSNDSQLQQTIHTSKLISRQLKSLYTGGSGSGNSSQVLQGKASK