AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene22859

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene22859.t1 MTR_7g116420 97.955 440 9 0 1 440 1 440 0.0 902
MS.gene22859.t1 MTR_1g079730 71.556 450 111 6 5 439 3 450 0.0 666
MS.gene22859.t1 MTR_8g088510 59.897 389 145 7 53 439 36 415 2.13e-172 489
MS.gene22859.t1 MTR_2g062610 56.331 387 164 4 53 439 44 425 1.11e-165 473
MS.gene22859.t1 MTR_8g069575 58.056 391 139 5 50 439 44 410 9.17e-163 465
MS.gene22859.t1 MTR_3g077430 57.033 391 144 7 49 439 98 464 1.17e-157 454
MS.gene22859.t1 MTR_5g033650 57.513 386 140 6 54 439 4 365 1.73e-154 442
MS.gene22859.t1 MTR_4g105400 56.757 370 137 6 70 439 11 357 1.84e-151 434
MS.gene22859.t1 MTR_2g062610 59.871 309 121 3 131 439 30 335 6.51e-143 411
MS.gene22859.t1 MTR_2g062610 59.871 309 121 3 131 439 37 342 1.08e-142 411
MS.gene22859.t1 MTR_8g069610 48.252 429 191 8 17 439 10 413 4.24e-138 402
MS.gene22859.t1 MTR_8g069600 51.031 388 159 7 58 439 30 392 2.32e-137 399
MS.gene22859.t1 MTR_3g095890 48.462 390 161 10 54 440 82 434 1.40e-125 371
MS.gene22859.t1 MTR_8g069720 41.244 434 219 7 12 439 3 406 1.89e-119 354
MS.gene22859.t1 MTR_8g069580 43.224 428 194 8 17 439 10 393 1.16e-117 349
MS.gene22859.t1 MTR_8g069590 51.613 310 135 5 131 439 9 304 4.71e-111 329
MS.gene22859.t1 MTR_6g012620 42.716 405 203 11 48 439 21 409 4.13e-105 318
MS.gene22859.t1 MTR_8g069640 40.326 429 216 8 17 439 10 404 7.37e-105 317
MS.gene22859.t1 MTR_8g069660 40.093 429 212 10 17 439 10 399 2.11e-100 305
MS.gene22859.t1 MTR_8g069715 41.162 396 191 8 52 439 48 409 5.85e-99 302
MS.gene22859.t1 MTR_3g104000 38.517 418 234 8 31 437 8 413 8.90e-92 284
MS.gene22859.t1 MTR_3g104070 37.412 425 248 8 17 437 8 418 2.37e-91 283
MS.gene22859.t1 MTR_3g104030 38.876 427 232 8 17 437 8 411 8.59e-91 281
MS.gene22859.t1 MTR_2g093840 50.000 302 129 9 142 439 76 359 3.94e-88 273
MS.gene22859.t1 MTR_3g103980 36.674 439 243 10 11 438 18 432 3.71e-86 270
MS.gene22859.t1 MTR_1g016910 37.562 402 234 7 47 439 26 419 1.79e-82 260
MS.gene22859.t1 MTR_8g069695 43.825 251 134 4 191 439 13 258 2.50e-73 231
MS.gene22859.t1 MTR_2g073040 42.701 274 129 5 43 315 23 269 5.06e-70 223
MS.gene22859.t1 MTR_3g104030 39.879 331 174 8 123 437 26 347 7.90e-70 225
MS.gene22859.t1 MTR_2g073020 41.176 306 145 5 18 315 4 282 3.96e-69 221
MS.gene22859.t1 MTR_8g040850 31.667 420 242 15 30 436 3 390 9.29e-57 192
MS.gene22859.t1 MTR_8g040890 31.481 432 253 14 30 434 3 418 2.34e-55 189
MS.gene22859.t1 MTR_5g078580 33.516 364 206 9 54 415 36 365 6.57e-54 185
MS.gene22859.t1 MTR_5g094280 35.577 312 161 11 52 362 18 290 2.29e-49 171
MS.gene22859.t1 MTR_3g108680 29.496 417 242 13 26 436 16 386 3.70e-49 172
MS.gene22859.t1 MTR_8g104480 32.615 371 196 16 77 434 8 337 2.23e-47 166
MS.gene22859.t1 MTR_5g092260 27.991 443 246 13 4 436 5 384 3.85e-44 159
MS.gene22859.t1 MTR_2g046390 32.982 379 195 15 68 434 37 368 9.00e-43 155
MS.gene22859.t1 MTR_8g069630 51.587 126 59 2 315 439 4 128 1.93e-40 141
MS.gene22859.t1 MTR_8g012985 37.200 250 137 11 202 440 2 242 7.61e-39 140
MS.gene22859.t1 MTR_8g069750 50.442 113 56 0 248 360 151 263 7.43e-38 139
MS.gene22859.t1 MTR_2g046300 32.454 379 182 16 68 434 37 353 2.58e-36 137
MS.gene22859.t1 MTR_2g046520 36.100 241 135 10 204 433 4 236 7.89e-34 127
MS.gene22859.t1 MTR_2g046430 36.100 241 135 10 204 433 4 236 6.02e-33 125
MS.gene22859.t1 MTR_5g065260 34.555 191 117 6 203 393 1 183 5.65e-28 111
MS.gene22859.t1 MTR_2g046390 28.117 377 173 14 68 434 37 325 2.72e-25 106
MS.gene22859.t1 MTR_0009s0020 36.232 138 82 4 249 386 18 149 1.28e-21 91.3
MS.gene22859.t1 MTR_2g073050 51.429 70 33 1 370 439 1 69 1.98e-19 82.4
MS.gene22859.t1 MTR_7g072230 32.955 176 104 7 267 432 34 205 5.64e-19 85.5
MS.gene22859.t1 MTR_8g069950 60.000 55 22 0 267 321 58 112 1.26e-17 79.0
MS.gene22859.t1 MTR_8g069665 41.237 97 44 3 71 167 109 192 1.29e-14 72.8
MS.gene22859.t1 MTR_5g065920 56.897 58 21 2 192 249 9 62 3.80e-14 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene22859.t1 AT5G18460 72.030 404 106 4 38 440 33 430 0.0 600
MS.gene22859.t1 AT1G55360 54.651 430 180 8 11 439 6 421 2.47e-171 487
MS.gene22859.t1 AT3G13510 54.654 419 173 8 23 439 15 418 9.73e-170 483
MS.gene22859.t1 AT5G56530 53.721 430 183 7 11 439 5 419 4.81e-167 476
MS.gene22859.t1 AT5G56530 53.721 430 183 7 11 439 5 419 4.81e-167 476
MS.gene22859.t1 AT1G10750 58.462 390 138 5 50 439 101 466 3.54e-163 468
MS.gene22859.t1 AT1G23340 54.374 423 165 9 17 439 14 408 6.82e-163 465
MS.gene22859.t1 AT1G23340 54.374 423 165 9 17 439 14 408 6.82e-163 465
MS.gene22859.t1 AT5G50150 55.721 402 154 6 39 439 41 419 1.90e-160 459
MS.gene22859.t1 AT1G70550 57.179 390 143 8 50 439 99 464 4.11e-158 455
MS.gene22859.t1 AT2G44210 50.948 422 194 7 19 439 5 414 2.72e-157 451
MS.gene22859.t1 AT2G44210 50.957 418 168 7 53 439 33 444 6.33e-151 436
MS.gene22859.t1 AT2G44220 44.681 423 209 7 18 439 4 402 1.04e-133 390
MS.gene22859.t1 AT3G48230 51.042 384 164 9 58 439 11 372 3.20e-132 386
MS.gene22859.t1 AT2G44240 43.294 425 211 10 18 439 4 401 1.65e-123 365
MS.gene22859.t1 AT2G17750 45.063 395 197 9 45 439 21 395 2.23e-123 364
MS.gene22859.t1 AT2G19360 42.955 440 211 7 19 440 9 426 4.62e-123 364
MS.gene22859.t1 AT5G19170 43.257 393 191 7 46 434 1 365 2.41e-107 323
MS.gene22859.t1 AT2G44250 41.121 428 227 10 17 439 1 408 4.21e-107 323
MS.gene22859.t1 AT1G23340 54.044 272 99 8 44 315 39 284 1.61e-95 289
MS.gene22859.t1 AT2G44250 42.687 335 176 10 111 439 1 325 8.94e-83 258
MS.gene22859.t1 AT5G25950 39.000 400 218 10 54 439 44 431 5.19e-81 256
MS.gene22859.t1 AT2G20170 36.879 423 222 15 22 434 9 396 3.66e-74 238
MS.gene22859.t1 AT2G20170 36.879 423 222 15 22 434 9 396 3.66e-74 238
MS.gene22859.t1 AT5G25960 37.949 390 180 10 54 439 25 356 4.09e-70 226
MS.gene22859.t1 AT4G23370 33.652 419 245 12 25 434 7 401 2.16e-69 226
MS.gene22859.t1 AT4G23390 36.082 388 206 13 58 434 41 397 1.48e-67 221
MS.gene22859.t1 AT2G20170 34.434 424 216 15 21 434 8 379 1.99e-64 212
MS.gene22859.t1 AT4G23365 32.506 403 235 13 41 434 24 398 7.66e-64 211
MS.gene22859.t1 AT4G23355 32.741 394 232 13 47 434 32 398 2.25e-61 205
MS.gene22859.t1 AT1G70550 50.230 217 84 8 50 266 99 291 9.29e-61 199
MS.gene22859.t1 AT4G23350 31.095 402 244 12 41 434 24 400 1.86e-60 202
MS.gene22859.t1 AT4G23380 32.487 394 222 13 52 434 38 398 3.72e-59 199
MS.gene22859.t1 AT4G23360 32.506 403 236 14 41 434 24 399 4.55e-59 199
MS.gene22859.t1 AT1G10750 49.020 204 80 5 50 253 101 280 1.70e-52 180
MS.gene22859.t1 AT2G35250 34.417 369 182 13 75 436 24 339 1.32e-48 169
MS.gene22859.t1 AT4G17505 30.933 375 185 12 66 437 18 321 3.98e-45 159
MS.gene22859.t1 AT1G10190 29.907 428 256 16 19 435 1 395 5.82e-44 158
MS.gene22859.t1 AT4G15050 30.049 406 242 15 39 435 23 395 6.80e-44 158
MS.gene22859.t1 AT2G27320 34.579 321 159 10 77 395 30 301 1.31e-42 153
MS.gene22859.t1 AT5G46200 29.426 401 223 17 77 439 30 408 8.06e-40 147
MS.gene22859.t1 AT4G15053 30.691 391 232 14 54 435 42 402 1.06e-39 147
MS.gene22859.t1 AT4G10220 30.233 387 230 15 53 434 47 398 1.00e-38 144
MS.gene22859.t1 AT2G24950 29.111 450 259 22 6 435 1 410 2.95e-38 143
MS.gene22859.t1 AT5G25410 27.968 379 224 12 62 434 28 363 3.16e-37 140
MS.gene22859.t1 AT5G46810 30.108 372 216 15 78 436 11 351 4.86e-37 139
MS.gene22859.t1 AT4G23373 32.453 265 135 5 25 289 7 227 5.07e-37 137
MS.gene22859.t1 AT5G05030 29.124 388 218 15 59 436 19 359 5.38e-37 139
MS.gene22859.t1 AT5G60380 26.984 378 227 13 65 434 31 367 3.37e-36 137
MS.gene22859.t1 AT5G46820 30.163 368 209 13 78 434 17 347 1.51e-35 135
MS.gene22859.t1 AT4G17860 28.117 377 205 17 78 439 29 354 4.68e-32 125
MS.gene22859.t1 AT4G10210 27.358 424 260 16 6 419 1 386 5.38e-30 120
MS.gene22859.t1 AT2G38255 35.088 228 104 6 78 305 14 197 8.03e-30 115
MS.gene22859.t1 AT2G03930 56.522 92 40 0 190 281 42 133 3.51e-29 112
MS.gene22859.t1 AT4G23080 28.894 398 231 17 53 436 47 406 1.97e-28 116
MS.gene22859.t1 AT5G11660 29.836 305 182 12 139 436 25 304 8.52e-28 112
MS.gene22859.t1 AT5G25415 29.825 228 141 6 220 436 113 332 1.09e-21 95.9
MS.gene22859.t1 AT5G36680 27.653 311 186 13 134 434 46 327 3.82e-21 94.4
MS.gene22859.t1 AT5G36770 27.653 311 186 13 134 434 46 327 3.82e-21 94.4
MS.gene22859.t1 AT5G04937 29.648 199 113 7 245 436 22 200 3.53e-17 80.1
MS.gene22859.t1 AT5G37520 32.386 176 102 9 265 434 33 197 5.11e-15 73.9

Find 130 sgRNAs with CRISPR-Local

Find 197 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTCAAATTTGGATTCTTTC+TGG 0.084366 7.4:+1824504 MS.gene22859:CDS
AATTTAGTCTTTCTCAAATT+TGG 0.102248 7.4:+1824493 MS.gene22859:CDS
GCTAAATCTTTGTATGAATA+TGG 0.199278 7.4:+1823973 MS.gene22859:CDS
CTCTAATAATTGTTTGTAAA+AGG 0.210392 7.4:-1822838 None:intergenic
TGGATTCTTTCTGGATCTTT+TGG 0.225879 7.4:+1824513 MS.gene22859:CDS
TCGCCGTTATTAACAACTTC+AGG 0.268540 7.4:-1824042 None:intergenic
TTGGACAACGTGTCCCATTT+TGG 0.271651 7.4:-1823903 None:intergenic
TGGCAAATGTGGCACCAAAA+TGG 0.279621 7.4:+1823889 MS.gene22859:CDS
GACCCAGATTATTCACTTAC+TGG 0.306757 7.4:+1825016 MS.gene22859:CDS
GCGATTGCGTTCACAAAATC+AGG 0.309262 7.4:+1824405 MS.gene22859:CDS
TTTGGTGACGGCACATTGAT+AGG 0.315027 7.4:+1825500 MS.gene22859:CDS
CACTTTGCTGAAGATGGCTT+TGG 0.334222 7.4:+1825644 MS.gene22859:CDS
TTGGAAAAGCAAGCTACTTC+AGG 0.347824 7.4:+1825663 MS.gene22859:CDS
GCCTATACTTCGTGTAGTTT+AGG 0.363508 7.4:-1822874 None:intergenic
TCCGGCCACTTTGCTGAAGA+TGG 0.363622 7.4:+1825638 MS.gene22859:CDS
CGTCCAGTAAGTGAATAATC+TGG 0.368717 7.4:-1825019 None:intergenic
ATAAGAAGAGAGAGGAGAAA+TGG 0.388996 7.4:-1825288 None:intergenic
TGCTACAACCTTCTTTGTGC+TGG 0.397788 7.4:+1825227 MS.gene22859:CDS
CCAACACACCTCCACTCAAA+TGG 0.398678 7.4:+1825613 MS.gene22859:CDS
TAGGTCAGTCCGGAGCTTTA+TGG 0.400753 7.4:+1824985 MS.gene22859:intron
AGAGCTCCTACAACAACAAA+TGG 0.403956 7.4:+1825774 MS.gene22859:CDS
ATAATATTACCTCTATAGTT+AGG 0.422015 7.4:-1822961 None:intergenic
TCGTGCCACCATGGTGCAAT+GGG 0.425828 7.4:+1825559 MS.gene22859:CDS
GGCAAATGTGGCACCAAAAT+GGG 0.428900 7.4:+1823890 MS.gene22859:CDS
ATAAACCTTCCAGATGAGTA+TGG 0.431310 7.4:-1825333 None:intergenic
CGCCGTTATTAACAACTTCA+GGG 0.431464 7.4:-1824041 None:intergenic
AAATCAGGGGAAGAGGTGTA+TGG 0.433416 7.4:+1824420 MS.gene22859:CDS
GGGAACTGGTGGATGAGATT+TGG 0.437839 7.4:+1825482 MS.gene22859:CDS
TGTGCCCCATTTGTTGTTGT+AGG 0.439039 7.4:-1825780 None:intergenic
ATATTCATATCCATTCCCTT+TGG 0.441719 7.4:-1823817 None:intergenic
GAGCATTGTGGATTGTTGCC+AGG 0.452796 7.4:-1825821 None:intergenic
GAGCTCCTACAACAACAAAT+GGG 0.454693 7.4:+1825775 MS.gene22859:CDS
AGGATCCACAACTTGGGAAC+TGG 0.461610 7.4:+1825468 MS.gene22859:intron
ATCGTGCCACCATGGTGCAA+TGG 0.461729 7.4:+1825558 MS.gene22859:CDS
AGTGACACATATCAAGCAAC+TGG 0.463842 7.4:+1825203 MS.gene22859:CDS
ATTCCCTTTGGCATCGTCGT+TGG 0.464017 7.4:-1823805 None:intergenic
CTAAATCTTTGTATGAATAT+GGG 0.468256 7.4:+1823974 MS.gene22859:CDS
AGTGGAGGTGTGTTGGCCAT+TGG 0.481123 7.4:-1825606 None:intergenic
TTTAAAGAATCACAAAATCC+AGG 0.482298 7.4:+1823256 MS.gene22859:CDS
TTGACATCACCATACTCATC+TGG 0.484373 7.4:+1825324 MS.gene22859:CDS
ATTGATTAGGATCCACAACT+TGG 0.486292 7.4:+1825461 MS.gene22859:intron
TCATTGACTACTTCCACCGT+TGG 0.487439 7.4:-1824471 None:intergenic
CAACACACCTCCACTCAAAT+GGG 0.487631 7.4:+1825614 MS.gene22859:CDS
TAGCATTGAAGCTGGTTGGC+AGG 0.488190 7.4:+1824551 MS.gene22859:CDS
AATGGGACACGTTGTCCAAA+GGG 0.489971 7.4:+1823907 MS.gene22859:CDS
GATGAGAGAAGTGGTAAGAG+TGG 0.497824 7.4:+1823859 MS.gene22859:CDS
AATCAGGGGAAGAGGTGTAT+GGG 0.502251 7.4:+1824421 MS.gene22859:CDS
TTAATAGCATTGAAGCTGGT+TGG 0.503786 7.4:+1824547 MS.gene22859:CDS
TGCACCGTGCTCCGCCGTAT+CGG 0.505620 7.4:-1823937 None:intergenic
TTGCACCATGGTGGCACGAT+CGG 0.507100 7.4:-1825555 None:intergenic
ATCCACCAGTTCCCAAGTTG+TGG 0.508328 7.4:-1825473 None:intergenic
CTGGGCTAAGGTTAAGATGT+TGG 0.509928 7.4:-1825729 None:intergenic
GTGGTGAACACTCGCGCCAA+TGG 0.510609 7.4:+1825590 MS.gene22859:CDS
AGCAAGAAGAAGATGAGGAG+TGG 0.515521 7.4:-1822801 None:intergenic
ACCTCCACTCAAATGGGTTC+CGG 0.515882 7.4:+1825620 MS.gene22859:CDS
CGTATCGGTACAGTTCCCTT+TGG 0.516871 7.4:-1823922 None:intergenic
ATTACCTCTATAGTTAGGAC+AGG 0.517511 7.4:-1822956 None:intergenic
CGCGGAGGTGAGACTGTTGT+CGG 0.520352 7.4:-1825705 None:intergenic
CATGGTGCAATGGGGTGGCG+AGG 0.521629 7.4:+1825568 MS.gene22859:CDS
AATAATTGTTTGTAAAAGGA+AGG 0.522506 7.4:-1822834 None:intergenic
TCTAGAGGAGATGAGAGAAG+TGG 0.524587 7.4:+1823850 MS.gene22859:CDS
AACGACGATGCCAAAGGGAA+TGG 0.525654 7.4:+1823807 MS.gene22859:CDS
GCACAAAACCAGCACAAAGA+AGG 0.529315 7.4:-1825235 None:intergenic
TTGTTACTTCAGAGTCAAGA+TGG 0.529370 7.4:+1823179 MS.gene22859:intron
ACAAATAGCAAGATAGCCAT+TGG 0.530075 7.4:+1825260 MS.gene22859:CDS
GCCATCTTCAGCAAAGTGGC+CGG 0.534756 7.4:-1825639 None:intergenic
CTGCTGTCACCATAAAGCTC+CGG 0.539948 7.4:-1824994 None:intergenic
AAGAAAGCAAGAAGAAGATG+AGG 0.541340 7.4:-1822806 None:intergenic
TTAGTAGTTTGAGACTTGAA+AGG 0.545306 7.4:+1822900 MS.gene22859:CDS
TTGGTGACGGCACATTGATA+GGG 0.548675 7.4:+1825501 MS.gene22859:CDS
AGCATTGTGGATTGTTGCCA+GGG 0.551399 7.4:-1825820 None:intergenic
GATCTTAATAGCATTGAAGC+TGG 0.552923 7.4:+1824543 MS.gene22859:CDS
TTGCACTAGTTAGGTCAGTC+CGG 0.552965 7.4:+1824975 MS.gene22859:intron
ACCTAAACTACACGAAGTAT+AGG 0.555495 7.4:+1822873 MS.gene22859:CDS
CAAAGCCATCTTCAGCAAAG+TGG 0.555709 7.4:-1825643 None:intergenic
ACGGCACATTGATAGGGTAC+TGG 0.556765 7.4:+1825507 MS.gene22859:CDS
GGTGCAATGGGGTGGCGAGG+TGG 0.560849 7.4:+1825571 MS.gene22859:CDS
GTCCAGTAAGTGAATAATCT+GGG 0.564075 7.4:-1825018 None:intergenic
AGGAGAAATGGAAGCTCCAA+TGG 0.564350 7.4:-1825276 None:intergenic
TATGGTGATGTCAAATTGCT+TGG 0.565653 7.4:-1825315 None:intergenic
CAGATTATTCACTTACTGGA+CGG 0.568692 7.4:+1825020 MS.gene22859:CDS
CCATTTGAGTGGAGGTGTGT+TGG 0.568898 7.4:-1825613 None:intergenic
AGGCCAACGACGATGCCAAA+GGG 0.574441 7.4:+1823802 MS.gene22859:CDS
AAATGGGACACGTTGTCCAA+AGG 0.575071 7.4:+1823906 MS.gene22859:CDS
CGATTGCGTTCACAAAATCA+GGG 0.576262 7.4:+1824406 MS.gene22859:CDS
GTTCACAAAATCAGGGGAAG+AGG 0.576386 7.4:+1824413 MS.gene22859:CDS
AAGGCCAACGACGATGCCAA+AGG 0.577437 7.4:+1823801 MS.gene22859:CDS
ATCCACAACTTGGGAACTGG+TGG 0.577654 7.4:+1825471 MS.gene22859:CDS
TTGATTAGGATCCACAACTT+GGG 0.578524 7.4:+1825462 MS.gene22859:intron
AAAAGCAACAATAAATGTGT+GGG 0.580116 7.4:+1824446 MS.gene22859:CDS
AATAAATGTGTGGGATCCAA+CGG 0.581407 7.4:+1824455 MS.gene22859:CDS
CAAAAGCAACAATAAATGTG+TGG 0.585339 7.4:+1824445 MS.gene22859:CDS
AAGTGTGTGAATAACTCCAC+TGG 0.587412 7.4:-1825530 None:intergenic
AATATTAATGAAGAGTCTAG+AGG 0.592197 7.4:+1823835 MS.gene22859:CDS
TGTCGGTGTCTACAATCTCA+AGG 0.593806 7.4:-1825688 None:intergenic
ACCTCTATAGTTAGGACAGG+GGG 0.594543 7.4:-1822953 None:intergenic
CTCGCCACCCCATTGCACCA+TGG 0.599661 7.4:-1825567 None:intergenic
GCCACCATGGTGCAATGGGG+TGG 0.599972 7.4:+1825563 MS.gene22859:CDS
CTTAGCCGATCGTGCCACCA+TGG 0.601641 7.4:+1825550 MS.gene22859:CDS
TAAGGTTAAGATGTTGGTCG+CGG 0.602338 7.4:-1825723 None:intergenic
CCGTTATTAACAACTTCAGG+GGG 0.604223 7.4:-1824039 None:intergenic
GAAGTTGTTAATAACGGCGA+AGG 0.604884 7.4:+1824045 MS.gene22859:CDS
CAAAGGGAACTGTACCGATA+CGG 0.605213 7.4:+1823923 MS.gene22859:CDS
TGAGACTTGAAAGGATTCAG+AGG 0.607933 7.4:+1822909 MS.gene22859:CDS
GGTTAAGATGTTGGTCGCGG+AGG 0.610177 7.4:-1825720 None:intergenic
TGGACACTATTATTGTTGAG+AGG 0.613110 7.4:+1822765 MS.gene22859:CDS
ATTGATAGGGTACTGGCCAG+TGG 0.614292 7.4:+1825514 MS.gene22859:CDS
TGTACCGATACGGCGGAGCA+CGG 0.614700 7.4:+1823933 MS.gene22859:CDS
TCGTTGCGACGGAATAAAAG+TGG 0.617469 7.4:-1824015 None:intergenic
TTACCTCTATAGTTAGGACA+GGG 0.618276 7.4:-1822955 None:intergenic
GCCGGAACCCATTTGAGTGG+AGG 0.619376 7.4:-1825621 None:intergenic
CATCACCATACTCATCTGGA+AGG 0.620289 7.4:+1825328 MS.gene22859:CDS
GTGGTGTGGCATGGCAAATG+TGG 0.628794 7.4:+1823878 MS.gene22859:CDS
AGTGATGATCACGAGCATTG+TGG 0.630196 7.4:-1825833 None:intergenic
GTGGCCGGAACCCATTTGAG+TGG 0.633212 7.4:-1825624 None:intergenic
ATCAGGGGAAGAGGTGTATG+GGG 0.633285 7.4:+1824422 MS.gene22859:CDS
GCCGTTATTAACAACTTCAG+GGG 0.633552 7.4:-1824040 None:intergenic
GTGGTAAGAGTGGTGTGGCA+TGG 0.656278 7.4:+1823869 MS.gene22859:CDS
GCCACCCCATTGCACCATGG+TGG 0.657695 7.4:-1825564 None:intergenic
TTGGCATTATAAGAAGAGAG+AGG 0.666431 7.4:-1825296 None:intergenic
GAGAAGTGGTAAGAGTGGTG+TGG 0.676091 7.4:+1823864 MS.gene22859:CDS
AGCTCCTACAACAACAAATG+GGG 0.676238 7.4:+1825776 MS.gene22859:CDS
AAATGTGTGGGATCCAACGG+TGG 0.688543 7.4:+1824458 MS.gene22859:CDS
TAATAACGGCGAAGGCCATG+AGG 0.692332 7.4:+1824053 MS.gene22859:CDS
AGGGAACTGTACCGATACGG+CGG 0.708061 7.4:+1823926 MS.gene22859:CDS
GATTGCGTTCACAAAATCAG+GGG 0.725289 7.4:+1824407 MS.gene22859:CDS
CGTGCCACCATGGTGCAATG+GGG 0.726243 7.4:+1825560 MS.gene22859:CDS
TGGTGGATGAGATTTGGTGA+CGG 0.732995 7.4:+1825488 MS.gene22859:CDS
TACCTCTATAGTTAGGACAG+GGG 0.733427 7.4:-1822954 None:intergenic
AATATGGGAAGAAACAACGA+CGG 0.747260 7.4:+1823989 MS.gene22859:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATATATATATATATAGCT+AGG - chr7.4:1823056-1823075 None:intergenic 10.0%
!!! TAATTAGAGATATATTTTAA+AGG - chr7.4:1824717-1824736 None:intergenic 10.0%
!!! TATAAATTTTTTAACAGAAA+AGG + chr7.4:1823782-1823801 MS.gene22859:intron 10.0%
!! ATATTGTTTAAATAATTGAG+AGG + chr7.4:1824330-1824349 MS.gene22859:intron 15.0%
!! TAATGAACATATAAGAATAA+AGG - chr7.4:1823135-1823154 None:intergenic 15.0%
!! TCGCATTTATAAATATAAAT+TGG + chr7.4:1824243-1824262 MS.gene22859:intron 15.0%
!! TTATATTTATAAATGCGATA+TGG - chr7.4:1824241-1824260 None:intergenic 15.0%
!!! ATTCATTTGTATTTATTTCT+TGG + chr7.4:1823692-1823711 MS.gene22859:intron 15.0%
!!! ATTTTGTGATTCTTTAAAAA+GGG - chr7.4:1823254-1823273 None:intergenic 15.0%
!!! CATTTGATTTTTTATTTGTT+TGG + chr7.4:1825082-1825101 MS.gene22859:intron 15.0%
!!! TTAGACTATAATCTTTTTTT+TGG - chr7.4:1824625-1824644 None:intergenic 15.0%
!!! TTTGCTCTAAATTTTTTTTA+AGG + chr7.4:1824139-1824158 MS.gene22859:intron 15.0%
!! AATAATTGTTTGTAAAAGGA+AGG - chr7.4:1822837-1822856 None:intergenic 20.0%
!! AATTGAGAGGAAAATAATTA+AGG + chr7.4:1824343-1824362 MS.gene22859:intron 20.0%
!! ATAATATTACCTCTATAGTT+AGG - chr7.4:1822964-1822983 None:intergenic 20.0%
!! CAACAGCAAAAAAAAAAAAA+AGG - chr7.4:1823494-1823513 None:intergenic 20.0%
!! CTAAATCTTTGTATGAATAT+GGG + chr7.4:1823974-1823993 MS.gene22859:CDS 20.0%
!! CTCTAATAATTGTTTGTAAA+AGG - chr7.4:1822841-1822860 None:intergenic 20.0%
!! GGGACAAATTTATAATATTA+AGG - chr7.4:1823376-1823395 None:intergenic 20.0%
!! TATATTTCATCACACATATA+TGG + chr7.4:1823281-1823300 MS.gene22859:intron 20.0%
!!! AATTTAGTCTTTCTCAAATT+TGG + chr7.4:1824493-1824512 MS.gene22859:CDS 20.0%
!!! CATATGCAAAAAGAATTTTT+TGG + chr7.4:1824765-1824784 MS.gene22859:intron 20.0%
!!! CTAAATTTTTTTTAAGGCAA+TGG + chr7.4:1824145-1824164 MS.gene22859:intron 20.0%
!!! CTTTTTTTTTTTTTTGCTGT+TGG + chr7.4:1823492-1823511 MS.gene22859:intron 20.0%
!!! GATTTTGTGATTCTTTAAAA+AGG - chr7.4:1823255-1823274 None:intergenic 20.0%
!!! GATTTTTTATTTGTTTGGTT+GGG + chr7.4:1825087-1825106 MS.gene22859:intron 20.0%
!!! TATAATCTGAAATTGAAGAT+TGG - chr7.4:1824593-1824612 None:intergenic 20.0%
!!! TATTTATTTCTTGGTTTGAA+AGG + chr7.4:1823701-1823720 MS.gene22859:intron 20.0%
!!! TGATTTTTTATTTGTTTGGT+TGG + chr7.4:1825086-1825105 MS.gene22859:intron 20.0%
!!! TTGTCAATTACTTATTGATT+AGG + chr7.4:1825448-1825467 MS.gene22859:intron 20.0%
!!! TTTTGTGATTCTTTAAAAAG+GGG - chr7.4:1823253-1823272 None:intergenic 20.0%
! AAAAGCAACAATAAATGTGT+GGG + chr7.4:1824446-1824465 MS.gene22859:CDS 25.0%
! AATATTAATGAAGAGTCTAG+AGG + chr7.4:1823835-1823854 MS.gene22859:CDS 25.0%
! GCTAAATCTTTGTATGAATA+TGG + chr7.4:1823973-1823992 MS.gene22859:CDS 25.0%
! TAACTACTATCCATTTATGT+TGG + chr7.4:1824197-1824216 MS.gene22859:intron 25.0%
! TAATAATAATACACACCTCA+TGG - chr7.4:1824071-1824090 None:intergenic 25.0%
! TTTAAAGAATCACAAAATCC+AGG + chr7.4:1823256-1823275 MS.gene22859:CDS 25.0%
!! ACAAGTTAGTGACATTATTT+TGG - chr7.4:1823585-1823604 None:intergenic 25.0%
!! ATTGTAACAGTTTTTGTTCT+GGG - chr7.4:1825750-1825769 None:intergenic 25.0%
!! TATTGTAACAGTTTTTGTTC+TGG - chr7.4:1825751-1825770 None:intergenic 25.0%
!!! TTTTGATAATTGCACTAGTT+AGG + chr7.4:1824966-1824985 MS.gene22859:intron 25.0%
!!! TTTTTTTTGCTGTTGGTTTT+TGG + chr7.4:1823499-1823518 MS.gene22859:intron 25.0%
AACAAAAACTAATCAACCCT+AGG - chr7.4:1823755-1823774 None:intergenic 30.0%
AATCCAATTAACTTACTCCT+AGG + chr7.4:1823735-1823754 MS.gene22859:intron 30.0%
ACTACTTCAACTCATTACAA+AGG - chr7.4:1823528-1823547 None:intergenic 30.0%
AGTAATGTTTCTTCTGAACT+TGG - chr7.4:1824870-1824889 None:intergenic 30.0%
ATATTCATATCCATTCCCTT+TGG - chr7.4:1823820-1823839 None:intergenic 30.0%
ATCCAATTAACTTACTCCTA+GGG + chr7.4:1823736-1823755 MS.gene22859:intron 30.0%
ATGTTATATGACGCAGTATA+AGG - chr7.4:1823551-1823570 None:intergenic 30.0%
CAAAAGCAACAATAAATGTG+TGG + chr7.4:1824445-1824464 MS.gene22859:CDS 30.0%
TCTCAAATTTGGATTCTTTC+TGG + chr7.4:1824504-1824523 MS.gene22859:CDS 30.0%
TGTGTGATGAAATATATACC+TGG - chr7.4:1823277-1823296 None:intergenic 30.0%
TTCAGAAGAAACATTACTCA+AGG + chr7.4:1824872-1824891 MS.gene22859:intron 30.0%
! ACACTAACTACTCATTTTAG+AGG - chr7.4:1823398-1823417 None:intergenic 30.0%
! ATTTCTTGGTTTGAAAGGAA+AGG + chr7.4:1823706-1823725 MS.gene22859:intron 30.0%
! CACTAACTACTCATTTTAGA+GGG - chr7.4:1823397-1823416 None:intergenic 30.0%
! GAATAGAAAACGTTTTGCTT+TGG - chr7.4:1823676-1823695 None:intergenic 30.0%
! TGGTAGTAATTCTTTTCGTT+TGG + chr7.4:1824263-1824282 MS.gene22859:intron 30.0%
! TTAGTAGTTTGAGACTTGAA+AGG + chr7.4:1822900-1822919 MS.gene22859:CDS 30.0%
! TTTCTTGGTTTGAAAGGAAA+GGG + chr7.4:1823707-1823726 MS.gene22859:intron 30.0%
!! ACTAACTACTCATTTTAGAG+GGG - chr7.4:1823396-1823415 None:intergenic 30.0%
AAATGCGATATGGACAGTTA+GGG - chr7.4:1824231-1824250 None:intergenic 35.0%
AACCCTAGGAGTAAGTTAAT+TGG - chr7.4:1823741-1823760 None:intergenic 35.0%
AAGAAAGCAAGAAGAAGATG+AGG - chr7.4:1822809-1822828 None:intergenic 35.0%
AATAAATGTGTGGGATCCAA+CGG + chr7.4:1824455-1824474 MS.gene22859:CDS 35.0%
AATATGGGAAGAAACAACGA+CGG + chr7.4:1823989-1824008 MS.gene22859:CDS 35.0%
ACAAATAGCAAGATAGCCAT+TGG + chr7.4:1825260-1825279 MS.gene22859:CDS 35.0%
ACCTAAACTACACGAAGTAT+AGG + chr7.4:1822873-1822892 MS.gene22859:CDS 35.0%
ATAAACCTTCCAGATGAGTA+TGG - chr7.4:1825336-1825355 None:intergenic 35.0%
ATAAGAAGAGAGAGGAGAAA+TGG - chr7.4:1825291-1825310 None:intergenic 35.0%
ATTACCTCTATAGTTAGGAC+AGG - chr7.4:1822959-1822978 None:intergenic 35.0%
ATTGATTAGGATCCACAACT+TGG + chr7.4:1825461-1825480 MS.gene22859:intron 35.0%
CAGATTATTCACTTACTGGA+CGG + chr7.4:1825020-1825039 MS.gene22859:CDS 35.0%
CATGAAACGCTAGTACAAAA+TGG - chr7.4:1823348-1823367 None:intergenic 35.0%
GTCCAGTAAGTGAATAATCT+GGG - chr7.4:1825021-1825040 None:intergenic 35.0%
TAAATGCGATATGGACAGTT+AGG - chr7.4:1824232-1824251 None:intergenic 35.0%
TGGACACTATTATTGTTGAG+AGG + chr7.4:1822765-1822784 MS.gene22859:CDS 35.0%
TTACCTCTATAGTTAGGACA+GGG - chr7.4:1822958-1822977 None:intergenic 35.0%
TTGATTAGGATCCACAACTT+GGG + chr7.4:1825462-1825481 MS.gene22859:intron 35.0%
TTGGCATTATAAGAAGAGAG+AGG - chr7.4:1825299-1825318 None:intergenic 35.0%
! AAGGCAATGGTCTCTATTTT+TGG + chr7.4:1824158-1824177 MS.gene22859:intron 35.0%
! ATTCTTTCTGGATCTTTTGG+TGG + chr7.4:1824516-1824535 MS.gene22859:CDS 35.0%
! CATTTTGTACTAGCGTTTCA+TGG + chr7.4:1823346-1823365 MS.gene22859:intron 35.0%
! CTTTAAAAAGGGGTGATCTA+AGG - chr7.4:1823243-1823262 None:intergenic 35.0%
! GATCTTAATAGCATTGAAGC+TGG + chr7.4:1824543-1824562 MS.gene22859:CDS 35.0%
! TATGGTGATGTCAAATTGCT+TGG - chr7.4:1825318-1825337 None:intergenic 35.0%
! TGGATTCTTTCTGGATCTTT+TGG + chr7.4:1824513-1824532 MS.gene22859:CDS 35.0%
! TTAATAGCATTGAAGCTGGT+TGG + chr7.4:1824547-1824566 MS.gene22859:CDS 35.0%
! TTGTTACTTCAGAGTCAAGA+TGG + chr7.4:1823179-1823198 MS.gene22859:intron 35.0%
!! AAGGAACTAATTTGCTTGAG+TGG + chr7.4:1824362-1824381 MS.gene22859:intron 35.0%
AAGTGTGTGAATAACTCCAC+TGG - chr7.4:1825533-1825552 None:intergenic 40.0%
AGAGCTCCTACAACAACAAA+TGG + chr7.4:1825774-1825793 MS.gene22859:CDS 40.0%
AGCTCCTACAACAACAAATG+GGG + chr7.4:1825776-1825795 MS.gene22859:CDS 40.0%
AGTGACACATATCAAGCAAC+TGG + chr7.4:1825203-1825222 MS.gene22859:CDS 40.0%
ATATGGACAGTTAGGGACAT+AGG - chr7.4:1824224-1824243 None:intergenic 40.0%
CCCCCTGAAGTTGTTAATAA+CGG + chr7.4:1824039-1824058 MS.gene22859:CDS 40.0%
CCGTTATTAACAACTTCAGG+GGG - chr7.4:1824042-1824061 None:intergenic 40.0%
CGATTGCGTTCACAAAATCA+GGG + chr7.4:1824406-1824425 MS.gene22859:CDS 40.0%
CGCCGTTATTAACAACTTCA+GGG - chr7.4:1824044-1824063 None:intergenic 40.0%
CGTCCAGTAAGTGAATAATC+TGG - chr7.4:1825022-1825041 None:intergenic 40.0%
GAAGTTGTTAATAACGGCGA+AGG + chr7.4:1824045-1824064 MS.gene22859:CDS 40.0%
GACCCAGATTATTCACTTAC+TGG + chr7.4:1825016-1825035 MS.gene22859:CDS 40.0%
GAGCTCCTACAACAACAAAT+GGG + chr7.4:1825775-1825794 MS.gene22859:CDS 40.0%
GATTGCGTTCACAAAATCAG+GGG + chr7.4:1824407-1824426 MS.gene22859:CDS 40.0%
GCCGTTATTAACAACTTCAG+GGG - chr7.4:1824043-1824062 None:intergenic 40.0%
GCCTATACTTCGTGTAGTTT+AGG - chr7.4:1822877-1822896 None:intergenic 40.0%
GGACATAGGACCAACATAAA+TGG - chr7.4:1824210-1824229 None:intergenic 40.0%
TAAGGTTAAGATGTTGGTCG+CGG - chr7.4:1825726-1825745 None:intergenic 40.0%
TACCTCTATAGTTAGGACAG+GGG - chr7.4:1822957-1822976 None:intergenic 40.0%
TCGCCGTTATTAACAACTTC+AGG - chr7.4:1824045-1824064 None:intergenic 40.0%
TGAGACTTGAAAGGATTCAG+AGG + chr7.4:1822909-1822928 MS.gene22859:CDS 40.0%
TTGACATCACCATACTCATC+TGG + chr7.4:1825324-1825343 MS.gene22859:CDS 40.0%
TTGGAAAAGCAAGCTACTTC+AGG + chr7.4:1825663-1825682 MS.gene22859:CDS 40.0%
! GGCACATACTTTTACTATGG+TGG + chr7.4:1825797-1825816 MS.gene22859:CDS 40.0%
! TGGGGCACATACTTTTACTA+TGG + chr7.4:1825794-1825813 MS.gene22859:CDS 40.0%
!! ACAGTTTTTGTTCTGGGCTA+AGG - chr7.4:1825744-1825763 None:intergenic 40.0%
AAATGGGACACGTTGTCCAA+AGG + chr7.4:1823906-1823925 MS.gene22859:CDS 45.0%
AATGGGACACGTTGTCCAAA+GGG + chr7.4:1823907-1823926 MS.gene22859:CDS 45.0%
ACCTCTATAGTTAGGACAGG+GGG - chr7.4:1822956-1822975 None:intergenic 45.0%
AGCAAGAAGAAGATGAGGAG+TGG - chr7.4:1822804-1822823 None:intergenic 45.0%
AGGAGAAATGGAAGCTCCAA+TGG - chr7.4:1825279-1825298 None:intergenic 45.0%
CAAAGCCATCTTCAGCAAAG+TGG - chr7.4:1825646-1825665 None:intergenic 45.0%
CAACACACCTCCACTCAAAT+GGG + chr7.4:1825614-1825633 MS.gene22859:CDS 45.0%
CACTTTGCTGAAGATGGCTT+TGG + chr7.4:1825644-1825663 MS.gene22859:CDS 45.0%
CATCACCATACTCATCTGGA+AGG + chr7.4:1825328-1825347 MS.gene22859:CDS 45.0%
CTGGGCTAAGGTTAAGATGT+TGG - chr7.4:1825732-1825751 None:intergenic 45.0%
GATGAGAGAAGTGGTAAGAG+TGG + chr7.4:1823859-1823878 MS.gene22859:CDS 45.0%
GCACAAAACCAGCACAAAGA+AGG - chr7.4:1825238-1825257 None:intergenic 45.0%
GCGATTGCGTTCACAAAATC+AGG + chr7.4:1824405-1824424 MS.gene22859:CDS 45.0%
GGCAAATGTGGCACCAAAAT+GGG + chr7.4:1823890-1823909 MS.gene22859:CDS 45.0%
GTTCACAAAATCAGGGGAAG+AGG + chr7.4:1824413-1824432 MS.gene22859:CDS 45.0%
TCGTTGCGACGGAATAAAAG+TGG - chr7.4:1824018-1824037 None:intergenic 45.0%
TCTAGAGGAGATGAGAGAAG+TGG + chr7.4:1823850-1823869 MS.gene22859:CDS 45.0%
TGCTACAACCTTCTTTGTGC+TGG + chr7.4:1825227-1825246 MS.gene22859:CDS 45.0%
TGGCAAATGTGGCACCAAAA+TGG + chr7.4:1823889-1823908 MS.gene22859:CDS 45.0%
TTGCACTAGTTAGGTCAGTC+CGG + chr7.4:1824975-1824994 MS.gene22859:intron 45.0%
! AAAAAGGGGTGATCTAAGGC+TGG - chr7.4:1823239-1823258 None:intergenic 45.0%
! AAATCAGGGGAAGAGGTGTA+TGG + chr7.4:1824420-1824439 MS.gene22859:CDS 45.0%
! AATCAGGGGAAGAGGTGTAT+GGG + chr7.4:1824421-1824440 MS.gene22859:CDS 45.0%
! AGCATTGTGGATTGTTGCCA+GGG - chr7.4:1825823-1825842 None:intergenic 45.0%
! CAAAGGGAACTGTACCGATA+CGG + chr7.4:1823923-1823942 MS.gene22859:CDS 45.0%
! TACTTTTACTATGGTGGCCC+TGG + chr7.4:1825803-1825822 MS.gene22859:CDS 45.0%
! TCATTGACTACTTCCACCGT+TGG - chr7.4:1824474-1824493 None:intergenic 45.0%
! TGTCGGTGTCTACAATCTCA+AGG - chr7.4:1825691-1825710 None:intergenic 45.0%
! TTGGACAACGTGTCCCATTT+TGG - chr7.4:1823906-1823925 None:intergenic 45.0%
!! TGGTGGATGAGATTTGGTGA+CGG + chr7.4:1825488-1825507 MS.gene22859:CDS 45.0%
!! TGTGCCCCATTTGTTGTTGT+AGG - chr7.4:1825783-1825802 None:intergenic 45.0%
!! TTGGTGACGGCACATTGATA+GGG + chr7.4:1825501-1825520 MS.gene22859:CDS 45.0%
!! TTTGGTGACGGCACATTGAT+AGG + chr7.4:1825500-1825519 MS.gene22859:CDS 45.0%
!!! TAATTTTTATAAGAATTAAT+TGG - chr7.4:1822989-1823008 None:intergenic 5.0%
AAATGTGTGGGATCCAACGG+TGG + chr7.4:1824458-1824477 MS.gene22859:CDS 50.0%
AACGACGATGCCAAAGGGAA+TGG + chr7.4:1823807-1823826 MS.gene22859:CDS 50.0%
ACGGCACATTGATAGGGTAC+TGG + chr7.4:1825507-1825526 MS.gene22859:CDS 50.0%
AGGATCCACAACTTGGGAAC+TGG + chr7.4:1825468-1825487 MS.gene22859:intron 50.0%
ATCCACAACTTGGGAACTGG+TGG + chr7.4:1825471-1825490 MS.gene22859:CDS 50.0%
ATCCACCAGTTCCCAAGTTG+TGG - chr7.4:1825476-1825495 None:intergenic 50.0%
ATTCCCTTTGGCATCGTCGT+TGG - chr7.4:1823808-1823827 None:intergenic 50.0%
CCAACACACCTCCACTCAAA+TGG + chr7.4:1825613-1825632 MS.gene22859:CDS 50.0%
CTGCTGTCACCATAAAGCTC+CGG - chr7.4:1824997-1825016 None:intergenic 50.0%
GAGAAGTGGTAAGAGTGGTG+TGG + chr7.4:1823864-1823883 MS.gene22859:CDS 50.0%
TAATAACGGCGAAGGCCATG+AGG + chr7.4:1824053-1824072 MS.gene22859:CDS 50.0%
TAGGTCAGTCCGGAGCTTTA+TGG + chr7.4:1824985-1825004 MS.gene22859:intron 50.0%
! ACCTCCACTCAAATGGGTTC+CGG + chr7.4:1825620-1825639 MS.gene22859:CDS 50.0%
! ATCAGGGGAAGAGGTGTATG+GGG + chr7.4:1824422-1824441 MS.gene22859:CDS 50.0%
! CCATTTGAGTGGAGGTGTGT+TGG - chr7.4:1825616-1825635 None:intergenic 50.0%
! CGTATCGGTACAGTTCCCTT+TGG - chr7.4:1823925-1823944 None:intergenic 50.0%
! GAGCATTGTGGATTGTTGCC+AGG - chr7.4:1825824-1825843 None:intergenic 50.0%
! GGGAACTGGTGGATGAGATT+TGG + chr7.4:1825482-1825501 MS.gene22859:CDS 50.0%
! TAGCATTGAAGCTGGTTGGC+AGG + chr7.4:1824551-1824570 MS.gene22859:CDS 50.0%
!! ATTGATAGGGTACTGGCCAG+TGG + chr7.4:1825514-1825533 MS.gene22859:CDS 50.0%
AAGGCCAACGACGATGCCAA+AGG + chr7.4:1823801-1823820 MS.gene22859:CDS 55.0%
AGGCCAACGACGATGCCAAA+GGG + chr7.4:1823802-1823821 MS.gene22859:CDS 55.0%
ATCGTGCCACCATGGTGCAA+TGG + chr7.4:1825558-1825577 MS.gene22859:CDS 55.0%
GCCATCTTCAGCAAAGTGGC+CGG - chr7.4:1825642-1825661 None:intergenic 55.0%
GCCCCCTGTCCTAACTATAG+AGG + chr7.4:1822952-1822971 MS.gene22859:CDS 55.0%
GGTTAAGATGTTGGTCGCGG+AGG - chr7.4:1825723-1825742 None:intergenic 55.0%
GTGGTAAGAGTGGTGTGGCA+TGG + chr7.4:1823869-1823888 MS.gene22859:CDS 55.0%
GTGGTGTGGCATGGCAAATG+TGG + chr7.4:1823878-1823897 MS.gene22859:CDS 55.0%
TCGTGCCACCATGGTGCAAT+GGG + chr7.4:1825559-1825578 MS.gene22859:CDS 55.0%
TTGCACCATGGTGGCACGAT+CGG - chr7.4:1825558-1825577 None:intergenic 55.0%
! AGGGAACTGTACCGATACGG+CGG + chr7.4:1823926-1823945 MS.gene22859:CDS 55.0%
! AGTGGAGGTGTGTTGGCCAT+TGG - chr7.4:1825609-1825628 None:intergenic 55.0%
! TCCGGCCACTTTGCTGAAGA+TGG + chr7.4:1825638-1825657 MS.gene22859:CDS 55.0%
CGCGGAGGTGAGACTGTTGT+CGG - chr7.4:1825708-1825727 None:intergenic 60.0%
CGTGCCACCATGGTGCAATG+GGG + chr7.4:1825560-1825579 MS.gene22859:CDS 60.0%
CTTAGCCGATCGTGCCACCA+TGG + chr7.4:1825550-1825569 MS.gene22859:CDS 60.0%
GCCGGAACCCATTTGAGTGG+AGG - chr7.4:1825624-1825643 None:intergenic 60.0%
GTGGCCGGAACCCATTTGAG+TGG - chr7.4:1825627-1825646 None:intergenic 60.0%
GTGGTGAACACTCGCGCCAA+TGG + chr7.4:1825590-1825609 MS.gene22859:CDS 60.0%
! TGTACCGATACGGCGGAGCA+CGG + chr7.4:1823933-1823952 MS.gene22859:CDS 60.0%
CATGGTGCAATGGGGTGGCG+AGG + chr7.4:1825568-1825587 MS.gene22859:CDS 65.0%
CTCGCCACCCCATTGCACCA+TGG - chr7.4:1825570-1825589 None:intergenic 65.0%
CTTCAGGGGGCTCGTTGCGA+CGG - chr7.4:1824029-1824048 None:intergenic 65.0%
GCCACCATGGTGCAATGGGG+TGG + chr7.4:1825563-1825582 MS.gene22859:CDS 65.0%
GCCACCCCATTGCACCATGG+TGG - chr7.4:1825567-1825586 None:intergenic 65.0%
TGCACCGTGCTCCGCCGTAT+CGG - chr7.4:1823940-1823959 None:intergenic 65.0%
GGTGCAATGGGGTGGCGAGG+TGG + chr7.4:1825571-1825590 MS.gene22859:CDS 70.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 1822764 1825847 1822764 ID=MS.gene22859
chr7.4 mRNA 1822764 1825847 1822764 ID=MS.gene22859.t1;Parent=MS.gene22859
chr7.4 exon 1822764 1822973 1822764 ID=MS.gene22859.t1.exon1;Parent=MS.gene22859.t1
chr7.4 CDS 1822764 1822973 1822764 ID=cds.MS.gene22859.t1;Parent=MS.gene22859.t1
chr7.4 exon 1823191 1823277 1823191 ID=MS.gene22859.t1.exon2;Parent=MS.gene22859.t1
chr7.4 CDS 1823191 1823277 1823191 ID=cds.MS.gene22859.t1;Parent=MS.gene22859.t1
chr7.4 exon 1823799 1824074 1823799 ID=MS.gene22859.t1.exon3;Parent=MS.gene22859.t1
chr7.4 CDS 1823799 1824074 1823799 ID=cds.MS.gene22859.t1;Parent=MS.gene22859.t1
chr7.4 exon 1824402 1824572 1824402 ID=MS.gene22859.t1.exon4;Parent=MS.gene22859.t1
chr7.4 CDS 1824402 1824572 1824402 ID=cds.MS.gene22859.t1;Parent=MS.gene22859.t1
chr7.4 exon 1824988 1825041 1824988 ID=MS.gene22859.t1.exon5;Parent=MS.gene22859.t1
chr7.4 CDS 1824988 1825041 1824988 ID=cds.MS.gene22859.t1;Parent=MS.gene22859.t1
chr7.4 exon 1825203 1825349 1825203 ID=MS.gene22859.t1.exon6;Parent=MS.gene22859.t1
chr7.4 CDS 1825203 1825349 1825203 ID=cds.MS.gene22859.t1;Parent=MS.gene22859.t1
chr7.4 exon 1825470 1825847 1825470 ID=MS.gene22859.t1.exon7;Parent=MS.gene22859.t1
chr7.4 CDS 1825470 1825847 1825470 ID=cds.MS.gene22859.t1;Parent=MS.gene22859.t1
Gene Sequence

>MS.gene22859

ATGGACACTATTATTGTTGAGAGGAAGAAAAGAATTTCCACTCCTCATCTTCTTCTTGCTTTCTTGTTCACCTTCCTTTTACAAACAATTATTAGAGTTGAATGCATCAACCTAAACTACACGAAGTATAGGCAAGTTAGTAGTTTGAGACTTGAAAGGATTCAGAGGCACTTGAACAAGATTAACAAGCCCCCTGTCCTAACTATAGAGGTAATATTATTACCAATTAATTCTTATAAAAATTATTATTACTATTAATTCATAATATTAAATTTGTGTGTGCGCTAAGCCTAGCTATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATTGTTGTTTTTCCTTTATTCTTATATGTTCATTATAATTAAATACTTGCTATTGAATCTTGTTACTTCAGAGTCAAGATGGTGATCTCATAGATTGTGTTCACAAAAGAAAGCAACCAGCCTTAGATCACCCCTTTTTAAAGAATCACAAAATCCAGGTATATATTTCATCACACATATATGGTTATTCTACTTTTCTTCTTAAAGTTGATCTACATTTTAATTCCATTTTGTACTAGCGTTTCATGGCATTCCTTAATATTATAAATTTGTCCCCTCTAAAATGAGTAGTTAGTGTTAATCAAAAAAGATTTATATTTAATTTATTATTTTGCTATGCATGTGAAAATAAGTTTAATCACTAACTTGTTCCTTTTTTTTTTTTTTGCTGTTGGTTTTTGGACTCCTTTGTAATGAGTTGAAGTAGTCCTTATACTGCGTCATATAACATGACAATATTTTCCAAAATAATGTCACTAACTTGTTTTCAACAGATAATATTGCGTATCTCACTTTTTGATGAATTATATACTATGAAATATACATTTAAATACCAAAGCAAAACGTTTTCTATTCATTTGTATTTATTTCTTGGTTTGAAAGGAAAGGGTGTTGAATCCAATTAACTTACTCCTAGGGTTGATTAGTTTTTGTTTTAATATTATAAATTTTTTAACAGAAAAGGCCAACGACGATGCCAAAGGGAATGGATATGAATATTAATGAAGAGTCTAGAGGAGATGAGAGAAGTGGTAAGAGTGGTGTGGCATGGCAAATGTGGCACCAAAATGGGACACGTTGTCCAAAGGGAACTGTACCGATACGGCGGAGCACGGTGCATGATGTGTTGAGAGCTAAATCTTTGTATGAATATGGGAAGAAACAACGACGGTCACCACTTTTATTCCGTCGCAACGAGCCCCCTGAAGTTGTTAATAACGGCGAAGGCCATGAGGTGTGTATTATTATTAATCACTAGAGTAAATTATTTCATTGTTTTGAAAACTCATATTGCTATCTTTGCTCTAAATTTTTTTTAAGGCAATGGTCTCTATTTTTGGTCTTTGAATAGCTACTTAACTACTATCCATTTATGTTGGTCCTATGTCCCTAACTGTCCATATCGCATTTATAAATATAAATTGGTAGTAATTCTTTTCGTTTGGATAATATATTATTAATTCAACGAAGATTCTTAATTAATGTGTAAATATTGTTTAAATAATTGAGAGGAAAATAATTAAGGAACTAATTTGCTTGAGTGGTGCATATATACATGTAGCATGCGATTGCGTTCACAAAATCAGGGGAAGAGGTGTATGGGGCAAAAGCAACAATAAATGTGTGGGATCCAACGGTGGAAGTAGTCAATGAATTTAGTCTTTCTCAAATTTGGATTCTTTCTGGATCTTTTGGTGGCTCTGATCTTAATAGCATTGAAGCTGGTTGGCAGGTATTTTATTCTAAACTCCAATCTTCAATTTCAGATTATAGTCTCACTACCAAAAAAAAGATTATAGTCTAACATTTGTTTAAAATATAAATTAATCGACATTGTGTTAAAACTGATTGTAAATTATTATATTTATTTTATCCTTTAAAATATATCTCTAATTACTATGAAGTGTTTTTTTAATAGAGACATCATATGCAAAAAGAATTTTTTGGAAACTTTCATACACAATGACACATAATATACTATTTTTTAGTATTTGTAGAAAATGTAAAAAGAATTTTTAATATCTGACCAAGTTCAGAAGAAACATTACTCAAGGAAGAAAACAAATAAATAAATTTACAAAAAAAAAAAAAAAAACTAATCATGTGTATAAATAGTTTCTGAAGCTTTTGATAATTGCACTAGTTAGGTCAGTCCGGAGCTTTATGGTGACAGCAGACCCAGATTATTCACTTACTGGACGGTTAGCATTTCATCATCTTTTTCTCAGTTTTTTTTCATTGCATTTGATTTTTTATTTGTTTGGTTGGGTTTATTTCTTCATTTAATTTGATCTCTCATAAATATTCTCTAATGCACTCGTTCATAAAATTCTTGTTGATTAAACAATTTATAAGCACGCAGAGTGACACATATCAAGCAACTGGATGCTACAACCTTCTTTGTGCTGGTTTTGTGCAAACAAATAGCAAGATAGCCATTGGAGCTTCCATTTCTCCTCTCTCTTCTTATAATGCCAAGCAATTTGACATCACCATACTCATCTGGAAGGTTTATTTCTTATACATACAATTTTACTATATACAATAAAGTTTATTTTTTATACATTCTCATTGTAAAAAGATTATGTAGTATACATTGTCTAATAATTGTCAATTACTTATTGATTAGGATCCACAACTTGGGAACTGGTGGATGAGATTTGGTGACGGCACATTGATAGGGTACTGGCCAGTGGAGTTATTCACACACTTAGCCGATCGTGCCACCATGGTGCAATGGGGTGGCGAGGTGGTGAACACTCGCGCCAATGGCCAACACACCTCCACTCAAATGGGTTCCGGCCACTTTGCTGAAGATGGCTTTGGAAAAGCAAGCTACTTCAGGAACCTTGAGATTGTAGACACCGACAACAGTCTCACCTCCGCGACCAACATCTTAACCTTAGCCCAGAACAAAAACTGTTACAATATCAAGAGCTCCTACAACAACAAATGGGGCACATACTTTTACTATGGTGGCCCTGGCAACAATCCACAATGCTCGTGA
Protein sequence

>MS.gene22859.t1

MDTIIVERKKRISTPHLLLAFLFTFLLQTIIRVECINLNYTKYRQVSSLRLERIQRHLNKINKPPVLTIESQDGDLIDCVHKRKQPALDHPFLKNHKIQKRPTTMPKGMDMNINEESRGDERSGKSGVAWQMWHQNGTRCPKGTVPIRRSTVHDVLRAKSLYEYGKKQRRSPLLFRRNEPPEVVNNGEGHEHAIAFTKSGEEVYGAKATINVWDPTVEVVNEFSLSQIWILSGSFGGSDLNSIEAGWQVSPELYGDSRPRLFTYWTSDTYQATGCYNLLCAGFVQTNSKIAIGASISPLSSYNAKQFDITILIWKDPQLGNWWMRFGDGTLIGYWPVELFTHLADRATMVQWGGEVVNTRANGQHTSTQMGSGHFAEDGFGKASYFRNLEIVDTDNSLTSATNILTLAQNKNCYNIKSSYNNKWGTYFYYGGPGNNPQCS