AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene23006


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene23006.t1 MTR_4g104580 98.561 278 4 0 1 278 49 326 0.0 571
MS.gene23006.t1 MTR_7g108240 98.561 278 4 0 1 278 39 316 0.0 567
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene23006.t1 AT3G62940 65.169 267 76 4 20 276 52 311 2.39e-121 349
MS.gene23006.t1 AT3G62940 65.169 267 76 4 20 276 52 311 2.39e-121 349
MS.gene23006.t1 AT3G62940 65.169 267 76 4 20 276 68 327 3.45e-121 350
MS.gene23006.t1 AT3G62940 65.169 267 76 4 20 276 68 327 3.45e-121 350
MS.gene23006.t1 AT3G62940 65.169 267 76 4 20 276 68 327 3.45e-121 350
MS.gene23006.t1 AT5G67170 30.128 156 81 6 118 273 31 158 3.34e-12 66.2
MS.gene23006.t1 AT5G67170 30.128 156 81 6 118 273 31 158 3.34e-12 66.2

Find 66 sgRNAs with CRISPR-Local

Find 74 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTACCATATGCATGCTTT+TGG 0.248740 7.2:-6729197 MS.gene23006:CDS
ATCAGTTGGCCCACCTCTTT+GGG 0.250180 7.2:-6729544 MS.gene23006:CDS
AAATTCTCTGACAAACAAAA+TGG 0.255126 7.2:+6729443 None:intergenic
AATCAGTTGGCCCACCTCTT+TGG 0.262527 7.2:-6729545 MS.gene23006:CDS
CGAAGTTGTTGGTATGTATA+TGG 0.265726 7.2:+6729512 None:intergenic
TTGTCCCATTTGATCATATT+GGG 0.296913 7.2:+6729141 None:intergenic
AAAGAGCAATATGGACAATT+TGG 0.304162 7.2:-6729816 MS.gene23006:CDS
TTTGTCCCATTTGATCATAT+TGG 0.311395 7.2:+6729140 None:intergenic
AAGTAGAATCCACAGCAATA+TGG 0.313389 7.2:-6729364 MS.gene23006:CDS
GCCTAAAGCAGACAGTTCTT+TGG 0.342046 7.2:+6729862 None:intergenic
CCATTTGATCATATTGGGAT+TGG 0.377862 7.2:+6729146 None:intergenic
TTGCTGACCTTTGTATTCTC+AGG 0.380081 7.2:+6729758 None:intergenic
ATATCATAATATGCTTCTTC+AGG 0.382290 7.2:+6729306 None:intergenic
CTGTAAAGGCAGTGCCCATC+AGG 0.392181 7.2:+6729578 None:intergenic
CTGCCATTTCTCGAAGTTGT+TGG 0.400794 7.2:+6729501 None:intergenic
TCCTTCCCCATCTCCACATC+AGG 0.403765 7.2:+6729269 None:intergenic
AGTAGAATCCACAGCAATAT+GGG 0.405492 7.2:-6729363 MS.gene23006:CDS
CATTTGATCATATTGGGATT+GGG 0.415085 7.2:+6729147 None:intergenic
TAATGTCATTCTGCTCTGCT+TGG 0.417976 7.2:+6729678 None:intergenic
GTCCTTCCCTGATGTGGAGA+TGG 0.419141 7.2:-6729276 MS.gene23006:CDS
GCCAAAGAACTGTCTGCTTT+AGG 0.437434 7.2:-6729863 MS.gene23006:CDS
CGGAAATGAAAAGAGCAATA+TGG 0.438701 7.2:-6729825 MS.gene23006:CDS
ATACCAACAACTTCGAGAAA+TGG 0.439245 7.2:-6729504 MS.gene23006:CDS
TCCTTCCCTGATGTGGAGAT+GGG 0.451922 7.2:-6729275 MS.gene23006:CDS
GAAAGGAAGTTGAAGCCTCT+TGG 0.456629 7.2:-6729623 MS.gene23006:CDS
GCTGATGGTATTGGTTCGTC+TGG 0.457026 7.2:-6729236 MS.gene23006:CDS
TATAAATCAGCTGATGGTAT+TGG 0.465751 7.2:-6729245 MS.gene23006:CDS
TGCTAAGCTCAAGGAGAAGC+AGG 0.474324 7.2:-6729885 MS.gene23006:CDS
TTCAGGGTCCTTCCCTGATG+TGG 0.478905 7.2:-6729282 MS.gene23006:CDS
AATTCTCTGACAAACAAAAT+GGG 0.488222 7.2:+6729444 None:intergenic
ATGGTAAGAAAGCATGATAC+TGG 0.496674 7.2:+6729211 None:intergenic
AATTTGGTGAAGGCCATAGC+TGG 0.500352 7.2:-6729800 MS.gene23006:CDS
CAAGCCCAAAAGCATGCATA+TGG 0.502108 7.2:+6729192 None:intergenic
TTGTCAGAGAATTTGATTGA+AGG 0.523344 7.2:-6729434 MS.gene23006:CDS
GATTCTCAACAGCTCTGTAA+AGG 0.528906 7.2:+6729564 None:intergenic
ACAAGCAGCTAGAGGTAGCA+AGG 0.534438 7.2:-6729960 MS.gene23006:CDS
TTGTCCTCCCCATATTGCTG+TGG 0.559595 7.2:+6729355 None:intergenic
TGGTGAGCATTATAATTCTG+TGG 0.561039 7.2:-6729171 MS.gene23006:CDS
CAGAGCTGTTGAGAATCAGT+TGG 0.563261 7.2:-6729558 MS.gene23006:CDS
TTAGGCTATAGCAGTGGTAA+CGG 0.571066 7.2:-6729845 MS.gene23006:CDS
CCCCATCTCCACATCAGGGA+AGG 0.576306 7.2:+6729274 None:intergenic
TGGGGAGGACAACTAGAGCT+TGG 0.581684 7.2:-6729344 MS.gene23006:CDS
ACTAACAGATACTCCAGCTA+TGG 0.583680 7.2:+6729787 None:intergenic
CCTTCCCTGATGTGGAGATG+GGG 0.586628 7.2:-6729274 MS.gene23006:CDS
CTTCTTCAGGCAGTGAGTCA+AGG 0.591792 7.2:+6729319 None:intergenic
TCAACTCTCTGCTAAGCTCA+AGG 0.597792 7.2:-6729894 MS.gene23006:CDS
CCCTGATGTGGAGATGGGGA+AGG 0.605910 7.2:-6729270 MS.gene23006:CDS
CAGTTGGCCCACCTCTTTGG+GGG 0.609264 7.2:-6729542 MS.gene23006:CDS
AAGGAGTATAAATCAGCTGA+TGG 0.610891 7.2:-6729251 MS.gene23006:CDS
TGACATTATAAGTGATCGCA+CGG 0.613584 7.2:-6729663 MS.gene23006:CDS
TTGAAAATGAGAAACTCGAA+AGG 0.613680 7.2:-6729640 MS.gene23006:CDS
GTATGTGAAATAAAGCCTGA+TGG 0.628060 7.2:-6729593 MS.gene23006:CDS
CAATATGGACAATTTGGTGA+AGG 0.628539 7.2:-6729810 MS.gene23006:CDS
TGAGAATACAAAGGTCAGCA+AGG 0.636690 7.2:-6729756 MS.gene23006:CDS
AAATGGCAGCTGCTTACATG+AGG 0.636892 7.2:-6729487 MS.gene23006:CDS
TCACATACAGTCAAACCAAG+AGG 0.643986 7.2:+6729608 None:intergenic
GAATCCACAGCAATATGGGG+AGG 0.646499 7.2:-6729359 MS.gene23006:CDS
CCTTCCCCATCTCCACATCA+GGG 0.653564 7.2:+6729270 None:intergenic
TATGTGAAATAAAGCCTGAT+GGG 0.653902 7.2:-6729592 MS.gene23006:CDS
TCAGTTGGCCCACCTCTTTG+GGG 0.655302 7.2:-6729543 MS.gene23006:CDS
TCTCTGACAAACAAAATGGG+AGG 0.660756 7.2:+6729447 None:intergenic
GTAGAATCCACAGCAATATG+GGG 0.661003 7.2:-6729362 MS.gene23006:CDS
TTACATGAGGAAGCATACAT+CGG 0.677679 7.2:-6729474 MS.gene23006:CDS
TAGTCAACCTGAGAATACAA+AGG 0.678538 7.2:-6729765 MS.gene23006:CDS
AGGTCAGCAAGGCCAAACAG+AGG 0.705842 7.2:-6729745 MS.gene23006:CDS
TCTGCTTTAGGCTATAGCAG+TGG 0.730219 7.2:-6729851 MS.gene23006:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAGCATATTATGATATTTTC+AGG - chr7.2:6729823-6729842 MS.gene23006:CDS 20.0%
!!! AGCATATTATGATATTTTCA+GGG - chr7.2:6729824-6729843 MS.gene23006:CDS 20.0%
! AAATTCTCTGACAAACAAAA+TGG + chr7.2:6729682-6729701 None:intergenic 25.0%
! AATTCTCTGACAAACAAAAT+GGG + chr7.2:6729681-6729700 None:intergenic 25.0%
! ATATCATAATATGCTTCTTC+AGG + chr7.2:6729819-6729838 None:intergenic 25.0%
AAAAGCTAAGAAAAAGCAAG+TGG - chr7.2:6729192-6729211 MS.gene23006:CDS 30.0%
AAAGAGCAATATGGACAATT+TGG - chr7.2:6729306-6729325 MS.gene23006:CDS 30.0%
TATAAATCAGCTGATGGTAT+TGG - chr7.2:6729877-6729896 MS.gene23006:CDS 30.0%
TATGTGAAATAAAGCCTGAT+GGG - chr7.2:6729530-6729549 MS.gene23006:CDS 30.0%
TTGAAAATGAGAAACTCGAA+AGG - chr7.2:6729482-6729501 MS.gene23006:CDS 30.0%
TTGTCAGAGAATTTGATTGA+AGG - chr7.2:6729688-6729707 MS.gene23006:CDS 30.0%
AAGTAGAATCCACAGCAATA+TGG - chr7.2:6729758-6729777 MS.gene23006:CDS 35.0%
AGTAGAATCCACAGCAATAT+GGG - chr7.2:6729759-6729778 MS.gene23006:CDS 35.0%
ATACCAACAACTTCGAGAAA+TGG - chr7.2:6729618-6729637 MS.gene23006:CDS 35.0%
ATGAAAAAACAAGCAGCTAG+AGG - chr7.2:6729154-6729173 MS.gene23006:CDS 35.0%
CGAAGTTGTTGGTATGTATA+TGG + chr7.2:6729613-6729632 None:intergenic 35.0%
CGGAAATGAAAAGAGCAATA+TGG - chr7.2:6729297-6729316 MS.gene23006:CDS 35.0%
GTATGTGAAATAAAGCCTGA+TGG - chr7.2:6729529-6729548 MS.gene23006:CDS 35.0%
TAGTCAACCTGAGAATACAA+AGG - chr7.2:6729357-6729376 MS.gene23006:CDS 35.0%
TGACATTATAAGTGATCGCA+CGG - chr7.2:6729459-6729478 MS.gene23006:CDS 35.0%
TGGTGAGCATTATAATTCTG+TGG - chr7.2:6729951-6729970 MS.gene23006:CDS 35.0%
! AAGGAGTATAAATCAGCTGA+TGG - chr7.2:6729871-6729890 MS.gene23006:CDS 35.0%
! ATGGTAAGAAAGCATGATAC+TGG + chr7.2:6729914-6729933 None:intergenic 35.0%
! CAATATGGACAATTTGGTGA+AGG - chr7.2:6729312-6729331 MS.gene23006:CDS 35.0%
! CTTACCATATGCATGCTTTT+GGG - chr7.2:6729926-6729945 MS.gene23006:CDS 35.0%
! TCTTACCATATGCATGCTTT+TGG - chr7.2:6729925-6729944 MS.gene23006:CDS 35.0%
! TTACATGAGGAAGCATACAT+CGG - chr7.2:6729648-6729667 MS.gene23006:CDS 35.0%
ACTAACAGATACTCCAGCTA+TGG + chr7.2:6729338-6729357 None:intergenic 40.0%
AGCTAAGAAAAAGCAAGTGG+AGG - chr7.2:6729195-6729214 MS.gene23006:CDS 40.0%
GATTCTCAACAGCTCTGTAA+AGG + chr7.2:6729561-6729580 None:intergenic 40.0%
GTAGAATCCACAGCAATATG+GGG - chr7.2:6729760-6729779 MS.gene23006:CDS 40.0%
TAATGTCATTCTGCTCTGCT+TGG + chr7.2:6729447-6729466 None:intergenic 40.0%
TCACATACAGTCAAACCAAG+AGG + chr7.2:6729517-6729536 None:intergenic 40.0%
TCTCTGACAAACAAAATGGG+AGG + chr7.2:6729678-6729697 None:intergenic 40.0%
TGAGAATACAAAGGTCAGCA+AGG - chr7.2:6729366-6729385 MS.gene23006:CDS 40.0%
TTAGGCTATAGCAGTGGTAA+CGG - chr7.2:6729277-6729296 MS.gene23006:CDS 40.0%
TTGCTGACCTTTGTATTCTC+AGG + chr7.2:6729367-6729386 None:intergenic 40.0%
AAATGGCAGCTGCTTACATG+AGG - chr7.2:6729635-6729654 MS.gene23006:CDS 45.0%
CAGAGCTGTTGAGAATCAGT+TGG - chr7.2:6729564-6729583 MS.gene23006:CDS 45.0%
GAAAAAGCAAGTGGAGGAAG+AGG - chr7.2:6729201-6729220 MS.gene23006:CDS 45.0%
GAAAGGAAGTTGAAGCCTCT+TGG - chr7.2:6729499-6729518 MS.gene23006:CDS 45.0%
GCCAAAGAACTGTCTGCTTT+AGG - chr7.2:6729259-6729278 MS.gene23006:CDS 45.0%
GCCTAAAGCAGACAGTTCTT+TGG + chr7.2:6729263-6729282 None:intergenic 45.0%
TCAACTCTCTGCTAAGCTCA+AGG - chr7.2:6729228-6729247 MS.gene23006:CDS 45.0%
! AATTTGGTGAAGGCCATAGC+TGG - chr7.2:6729322-6729341 MS.gene23006:CDS 45.0%
! AGATTTTCCCCCAAAGAGGT+GGG + chr7.2:6729590-6729609 None:intergenic 45.0%
! ATGGAGATTTTCCCCCAAAG+AGG + chr7.2:6729594-6729613 None:intergenic 45.0%
! CAAGCCCAAAAGCATGCATA+TGG + chr7.2:6729933-6729952 None:intergenic 45.0%
! CATATGCATGCTTTTGGGCT+TGG - chr7.2:6729931-6729950 MS.gene23006:CDS 45.0%
! CTGCCATTTCTCGAAGTTGT+TGG + chr7.2:6729624-6729643 None:intergenic 45.0%
! TCTGCTTTAGGCTATAGCAG+TGG - chr7.2:6729271-6729290 MS.gene23006:CDS 45.0%
! TCTTTTGTCACGCCTCTGTT+TGG + chr7.2:6729392-6729411 None:intergenic 45.0%
AATCAGTTGGCCCACCTCTT+TGG - chr7.2:6729577-6729596 MS.gene23006:CDS 50.0%
ACAAGCAGCTAGAGGTAGCA+AGG - chr7.2:6729162-6729181 MS.gene23006:CDS 50.0%
ATCAGTTGGCCCACCTCTTT+GGG - chr7.2:6729578-6729597 MS.gene23006:CDS 50.0%
CTTCTTCAGGCAGTGAGTCA+AGG + chr7.2:6729806-6729825 None:intergenic 50.0%
GAATCCACAGCAATATGGGG+AGG - chr7.2:6729763-6729782 MS.gene23006:CDS 50.0%
TCCTTCCCTGATGTGGAGAT+GGG - chr7.2:6729847-6729866 MS.gene23006:CDS 50.0%
TGCTAAGCTCAAGGAGAAGC+AGG - chr7.2:6729237-6729256 MS.gene23006:CDS 50.0%
TTGTCCTCCCCATATTGCTG+TGG + chr7.2:6729770-6729789 None:intergenic 50.0%
! GAGATTTTCCCCCAAAGAGG+TGG + chr7.2:6729591-6729610 None:intergenic 50.0%
!! GCTGATGGTATTGGTTCGTC+TGG - chr7.2:6729886-6729905 MS.gene23006:CDS 50.0%
AGGTCAGCAAGGCCAAACAG+AGG - chr7.2:6729377-6729396 MS.gene23006:CDS 55.0%
CCTTCCCCATCTCCACATCA+GGG + chr7.2:6729855-6729874 None:intergenic 55.0%
CCTTCCCTGATGTGGAGATG+GGG - chr7.2:6729848-6729867 MS.gene23006:CDS 55.0%
CTGTAAAGGCAGTGCCCATC+AGG + chr7.2:6729547-6729566 None:intergenic 55.0%
GTCCTTCCCTGATGTGGAGA+TGG - chr7.2:6729846-6729865 MS.gene23006:CDS 55.0%
TCAGTTGGCCCACCTCTTTG+GGG - chr7.2:6729579-6729598 MS.gene23006:CDS 55.0%
TCCTTCCCCATCTCCACATC+AGG + chr7.2:6729856-6729875 None:intergenic 55.0%
TGGGGAGGACAACTAGAGCT+TGG - chr7.2:6729778-6729797 MS.gene23006:CDS 55.0%
TTCAGGGTCCTTCCCTGATG+TGG - chr7.2:6729840-6729859 MS.gene23006:CDS 55.0%
CAGTTGGCCCACCTCTTTGG+GGG - chr7.2:6729580-6729599 MS.gene23006:CDS 60.0%
CCCCATCTCCACATCAGGGA+AGG + chr7.2:6729851-6729870 None:intergenic 60.0%
CCCTGATGTGGAGATGGGGA+AGG - chr7.2:6729852-6729871 MS.gene23006:CDS 60.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 6729154 6729990 6729154 ID=MS.gene23006
chr7.2 mRNA 6729154 6729990 6729154 ID=MS.gene23006.t1;Parent=MS.gene23006
chr7.2 exon 6729154 6729990 6729154 ID=MS.gene23006.t1.exon1;Parent=MS.gene23006.t1
chr7.2 CDS 6729154 6729990 6729154 ID=cds.MS.gene23006.t1;Parent=MS.gene23006.t1
Gene Sequence

>MS.gene23006

ATGAAAAAACAAGCAGCTAGAGGTAGCAAGGCTGAGCAAAAAGCTAAGAAAAAGCAAGTGGAGGAAGAGGTTTCTCAACTCTCTGCTAAGCTCAAGGAGAAGCAGGCCAAAGAACTGTCTGCTTTAGGCTATAGCAGTGGTAACGGAAATGAAAAGAGCAATATGGACAATTTGGTGAAGGCCATAGCTGGAGTATCTGTTAGTAGTCAACCTGAGAATACAAAGGTCAGCAAGGCCAAACAGAGGCGTGACAAAAGAGCTCAACAAGAAGCAGAGAGAGAACAGAGAATCCAAGCAGAGCAGAATGACATTATAAGTGATCGCACGGTTGAAAATGAGAAACTCGAAAGGAAGTTGAAGCCTCTTGGTTTGACTGTATGTGAAATAAAGCCTGATGGGCACTGCCTTTACAGAGCTGTTGAGAATCAGTTGGCCCACCTCTTTGGGGGAAAATCTCCATATACATACCAACAACTTCGAGAAATGGCAGCTGCTTACATGAGGAAGCATACATCGGATTTCCTCCCATTTTGTTTGTCAGAGAATTTGATTGAAGGTGATTCTGATGAATCGATTGCTCAAAAGTTTGAAAATTATTGCAAAGAAGTAGAATCCACAGCAATATGGGGAGGACAACTAGAGCTTGGTGCCTTGACTCACTGCCTGAAGAAGCATATTATGATATTTTCAGGGTCCTTCCCTGATGTGGAGATGGGGAAGGAGTATAAATCAGCTGATGGTATTGGTTCGTCTGGTTCCAGTATCATGCTTTCTTACCATATGCATGCTTTTGGGCTTGGTGAGCATTATAATTCTGTGGTCCCAATCCCAATATGA

Protein sequence

>MS.gene23006.t1

MKKQAARGSKAEQKAKKKQVEEEVSQLSAKLKEKQAKELSALGYSSGNGNEKSNMDNLVKAIAGVSVSSQPENTKVSKAKQRRDKRAQQEAEREQRIQAEQNDIISDRTVENEKLERKLKPLGLTVCEIKPDGHCLYRAVENQLAHLFGGKSPYTYQQLREMAAAYMRKHTSDFLPFCLSENLIEGDSDESIAQKFENYCKEVESTAIWGGQLELGALTHCLKKHIMIFSGSFPDVEMGKEYKSADGIGSSGSSIMLSYHMHAFGLGEHYNSVVPIPI