AlfalfaGEDB | MS.gene24262

M. sativa cultivar XinJiangDaYe / MS.gene24262

KEGG: K19600
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: PF00646.36 PF01167.21

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene24262.t1	XP_013466123.1	93.9	413	15	2	1	403	10	422	4.90E-220	773.5

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene24262.t1	Q6Z2G9	61.0	428	133	8	1	403	10	428	4.5e-137	489.2

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene24262.t1	A0A072VEB5	93.9	413	15	2	1	403	10	422	3.5e-220	773.5

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene24262.t1	TF	TUB

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
MS.gene010523	MS.gene24262	PPI
MS.gene010525	MS.gene24262	PPI
MS.gene012024	MS.gene24262	PPI
MS.gene02443	MS.gene24262	PPI
MS.gene02464	MS.gene24262	PPI
MS.gene02465	MS.gene24262	PPI
MS.gene02475	MS.gene24262	PPI
MS.gene59384	MS.gene24262	PPI
MS.gene76417	MS.gene24262	PPI
MS.gene87465	MS.gene24262	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene24262.t1	MTR_1g022370	95.884	413	7	2	1	403	10	422	0.0	816
MS.gene24262.t1	MTR_5g020580	62.892	415	134	5	1	403	10	416	0.0	529
MS.gene24262.t1	MTR_8g077195	59.903	414	144	6	1	403	10	412	2.37e-172	488
MS.gene24262.t1	MTR_2g020280	58.630	365	141	4	46	403	32	393	6.29e-148	425
MS.gene24262.t1	MTR_1g098790	49.771	436	175	7	1	403	7	431	4.06e-145	419
MS.gene24262.t1	MTR_2g007930	50.816	429	177	7	1	403	7	427	2.91e-144	417
MS.gene24262.t1	MTR_1g088960	51.613	403	177	7	11	403	8	402	1.31e-136	397
MS.gene24262.t1	MTR_2g076200	49.580	357	159	4	47	403	36	371	9.11e-122	358
MS.gene24262.t1	MTR_2g020280	58.249	297	114	4	114	403	1	294	3.61e-117	343
MS.gene24262.t1	MTR_8g077675	47.805	410	176	12	1	396	10	395	3.18e-114	339

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene24262.t1	AT1G47270	61.990	392	127	6	19	403	12	388	3.09e-167	474
MS.gene24262.t1	AT1G47270	61.990	392	127	6	19	403	37	413	4.95e-167	474
MS.gene24262.t1	AT1G47270	61.990	392	127	6	19	403	36	412	6.35e-167	474
MS.gene24262.t1	AT2G18280	55.528	407	154	6	1	403	10	393	1.63e-152	437
MS.gene24262.t1	AT2G18280	55.528	407	154	6	1	403	10	393	1.63e-152	437
MS.gene24262.t1	AT2G47900	51.220	410	183	4	1	403	7	406	4.06e-146	421
MS.gene24262.t1	AT2G47900	51.220	410	183	4	1	403	7	406	4.06e-146	421
MS.gene24262.t1	AT2G47900	51.095	411	183	5	1	403	7	407	2.79e-144	416
MS.gene24262.t1	AT1G25280	49.774	442	180	7	1	403	7	445	1.57e-141	410
MS.gene24262.t1	AT1G76900	48.359	457	174	7	1	403	7	455	2.95e-138	403

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Find 85 sgRNAs with CRISPR-Local

Find 110 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGCCAGAAGTTGTTGAATT+TGG	0.203153	1.1:+1921340	MS.gene24262:CDS
AAGGAGATGAAAGAGGGAAT+TGG	0.461217	1.1:+1919911	MS.gene24262:CDS
AAGGCTTGAAAAGCAGAGAG+AGG	0.664732	1.1:-1921607	None:intergenic
AAGTTGCAAGTTGCAACAAA+AGG	0.506772	1.1:+1921102	MS.gene24262:CDS
ACCAAGATATAAACTATATG+TGG	0.517704	1.1:-1920427	None:intergenic
ACCAAGTTCACGATCTTAGA+TGG	0.416091	1.1:+1921049	MS.gene24262:CDS
ACCATCTAAGATCGTGAACT+TGG	0.451643	1.1:-1921050	None:intergenic
ACTAAGCATATGCATAGACA+TGG	0.617362	1.1:+1919959	MS.gene24262:CDS
ACTATTAGACATAATTCAAA+GGG	0.516941	1.1:+1920087	MS.gene24262:CDS
AGAACCTAGCCAAAATGTTT+CGG	0.455535	1.1:+1921510	MS.gene24262:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AGAGCTAACAATACTTACAT+TGG	+	chr1.1:1920917-1920936	MS.gene24262:CDS	30.0%
ATTAGACATAATTCAAAGGG+TGG	+	chr1.1:1920090-1920109	MS.gene24262:CDS	30.0%
ATTATGGAGAGACATAACAA+AGG	+	chr1.1:1920174-1920193	MS.gene24262:CDS	30.0%
CACAAATCGAGAATAGTTAA+CGG	-	chr1.1:1920741-1920760	None:intergenic	30.0%
TTATGGAGAGACATAACAAA+GGG	+	chr1.1:1920175-1920194	MS.gene24262:CDS	30.0%
AAGCCAGAAGTTGTTGAATT+TGG	+	chr1.1:1921340-1921359	MS.gene24262:CDS	35.0%
AAGTTGCAAGTTGCAACAAA+AGG	+	chr1.1:1921102-1921121	MS.gene24262:CDS	35.0%
ACAATTTGGAAAAATCGGGA+AGG	+	chr1.1:1921558-1921577	MS.gene24262:CDS	35.0%
ACTAAGCATATGCATAGACA+TGG	+	chr1.1:1919959-1919978	MS.gene24262:CDS	35.0%
AGAACCTAGCCAAAATGTTT+CGG	+	chr1.1:1921510-1921529	MS.gene24262:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1.1	gene	1919908	1921675	1919908	ID=MS.gene24262
chr1.1	mRNA	1919908	1921675	1919908	ID=MS.gene24262.t1;Parent=MS.gene24262
chr1.1	exon	1919908	1920255	1919908	ID=MS.gene24262.t1.exon1;Parent=MS.gene24262.t1
chr1.1	CDS	1919908	1920255	1919908	ID=cds.MS.gene24262.t1;Parent=MS.gene24262.t1
chr1.1	exon	1920372	1920459	1920372	ID=MS.gene24262.t1.exon2;Parent=MS.gene24262.t1
chr1.1	CDS	1920372	1920459	1920372	ID=cds.MS.gene24262.t1;Parent=MS.gene24262.t1
chr1.1	exon	1920816	1920948	1920816	ID=MS.gene24262.t1.exon3;Parent=MS.gene24262.t1
chr1.1	CDS	1920816	1920948	1920816	ID=cds.MS.gene24262.t1;Parent=MS.gene24262.t1
chr1.1	exon	1921033	1921675	1921033	ID=MS.gene24262.t1.exon4;Parent=MS.gene24262.t1
chr1.1	CDS	1921033	1921675	1921033	ID=cds.MS.gene24262.t1;Parent=MS.gene24262.t1

Gene Sequence

>MS.gene24262

ATGAAGGAGATGAAAGAGGGAATTGGCAACATGTATAGAAGAGGTGTAGAAACTAAGCATATGCATAGACATGGAAAATCTCACATAGCACCAGAATGTTCCTCACCAATATCACTATCAGCACCAACAACACCATCATCATCATCAGAGAGTCAATGGGCTAATTTACCTCCTGAGTTACTATTAGACATAATTCAAAGGGTGGAAGCTAGTGAAACCACTTGGCCATCTCGCCGAGCACTTGTAGCTTGTGCCTCAGTTTGTAGATTATGGAGAGACATAACAAAGGGTGTTGTTAAGACACTTGAACAGTGTGGCTGGATCACTTTCCCTATATCACTGAAGCAGGTAATTTTTTTCACTTTCTTTATGTATTTGATTTGATGTAATTTTTGTTTTAAGCTTGGAAGAACATGGTGTATAGTACTAAATGTGTGATTACTGTCTTTTATTTTGTTTTTCAGCCTGCTCCAAGAGACAATCCAATTCAGTGTTTTATTAAGAGAGAAAGGGCTACTTCCACATATAGTTTATATCTTGGTCTAAGCCCTGGTAAGATTGTTGTGCTCACATGAAATTATTCTCTATTTGGTAACAAAATTTTGTTTGGATTTTTGTAAAATAATACTAAGACTTCTCTTTGAATCAAAAGAAGTATGTAGTTGTTAAAACTTGAATTACCTTCTTTTAGGCTTTTTTTTTTTTTTTTTGTAGTATTCTTTTTCATCTTCTGTTTTCCAGAGTAAACTTACAAATCAATCGCTAAACTATCACATTCTTTTTCATCCTTCTTTTTTTAGTCTTTGAACTATGAAAATGTAATAAATAGTCCGTTAACTATTCTCGATTTGTGAAGGTAGTCTCTAAATTACCATATTTGTGAATTGTGTTATTGTATGTATTTGCAGCTCTTTCTGGTGATATGAGCAAACTTCTCTTGGCAGCAAAGAAGATTAGAAGAGCAACATGCACAGAATTTTTAATATCATTGGTTTCTGATGATTTCTCTAGAGCTAACAATACTTACATTGGAAAGTTAAGGTTAGTGTCCTCAATCAATCCACATCTTTTCATGGTTAATGCATTATAATCTACTGCTTAGTTAATCATTAAGCAAAATAACAGGTCTAATTTTCTCGGCACCAAGTTCACGATCTTAGATGGTCATCCTCCACATGGTTCTTCGCTTCCATCAAGTTGCAAGTTGCAACAAAAGGTCCATATGAAGCAAGTACTTCCAAGGGCTGCTCCTGCTAACTATAAAGTAGCAACTGTATCTTATGAACTCAATGTTCTTCGTACGAGAGGTCCAAGGCGAATGCGGAGCACGATGCACTTAATCCCCATATCCTCAGTCCAAGAAGGAGGAACTGCTCCTACTCCTATGGAATTTACCAATTGCCACAATGAGCATGAATCTGCAAAAGAAAAGAAGCCAGAAGTTGTTGAATTTGGCTCAACTTGCACTGATTTTGCAAGAGAGCCTCTGATTTTGAAAAACAAAGCACCAAGATGGCACGAACAACTGCAATGTTGGTGTTTGAATTTCAAGGGACGGGTTACAGTGGCTTCGGTGAAGAATTTTCAGCTTGTAGCAGCTGCAGAACCTAGCCAAAATGTTTCGGCTGCAGAACAAGAAAAAGTTATACTACAATTTGGAAAAATCGGGAAGGACATATTCACCATGGATTATCGATATCCTCTCTCTGCTTTTCAAGCCTTTGCAATCTGTTTGAGCAGCTTTGACACAAAACCAGCTTGTGAATGA

Protein sequence

>MS.gene24262.t1

MKEMKEGIGNMYRRGVETKHMHRHGKSHIAPECSSPISLSAPTTPSSSSESQWANLPPELLLDIIQRVEASETTWPSRRALVACASVCRLWRDITKGVVKTLEQCGWITFPISLKQPAPRDNPIQCFIKRERATSTYSLYLGLSPALSGDMSKLLLAAKKIRRATCTEFLISLVSDDFSRANNTYIGKLRSNFLGTKFTILDGHPPHGSSLPSSCKLQQKVHMKQVLPRAAPANYKVATVSYELNVLRTRGPRRMRSTMHLIPISSVQEGGTAPTPMEFTNCHNEHESAKEKKPEVVEFGSTCTDFAREPLILKNKAPRWHEQLQCWCLNFKGRVTVASVKNFQLVAAAEPSQNVSAAEQEKVILQFGKIGKDIFTMDYRYPLSAFQAFAICLSSFDTKPACE