AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene055591


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene055591.t1 MTR_2g098160 74.332 187 33 2 1 187 1 172 3.43e-97 279
MS.gene055591.t1 MTR_4g065570 85.350 157 3 1 31 187 1 137 2.01e-92 266
MS.gene055591.t1 MTR_7g092400 57.297 185 63 3 3 187 92 260 2.34e-70 214
MS.gene055591.t1 MTR_0249s0070 55.676 185 63 3 3 187 4 169 2.61e-68 206
MS.gene055591.t1 MTR_0249s0070 55.676 185 63 3 3 187 12 177 3.10e-68 206
MS.gene055591.t1 MTR_7g104320 40.217 184 92 5 4 187 3 168 9.61e-40 133
MS.gene055591.t1 MTR_4g053440 39.674 184 95 4 4 187 3 170 1.71e-39 132
MS.gene055591.t1 MTR_2g086190 39.884 173 72 4 15 187 10 150 5.84e-38 128
MS.gene055591.t1 MTR_1g060380 42.703 185 74 6 4 187 3 156 8.66e-35 120
MS.gene055591.t1 MTR_8g036980 65.574 61 21 0 127 187 91 151 1.64e-26 99.0
MS.gene055591.t1 MTR_3g028010 59.016 61 25 0 127 187 103 163 1.66e-23 91.7
MS.gene055591.t1 MTR_1g100773 30.814 172 82 5 15 185 3 138 1.09e-22 89.0
MS.gene055591.t1 MTR_7g114920 30.000 170 77 4 15 184 3 130 3.90e-22 87.4
MS.gene055591.t1 MTR_3g025570 72.727 44 12 0 122 165 4 47 2.80e-19 79.3
MS.gene055591.t1 MTR_2g054650 30.409 171 70 4 15 185 4 125 1.85e-17 75.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene055591.t1 AT1G51200 59.358 187 62 4 1 187 1 173 5.40e-64 195
MS.gene055591.t1 AT1G51200 59.358 187 62 4 1 187 1 173 5.40e-64 195
MS.gene055591.t1 AT1G51200 59.358 187 62 4 1 187 1 173 5.40e-64 195
MS.gene055591.t1 AT1G51200 59.358 187 62 4 1 187 1 173 5.40e-64 195
MS.gene055591.t1 AT2G36320 41.573 178 76 3 12 187 10 161 1.65e-42 140
MS.gene055591.t1 AT2G27580 39.306 173 86 3 15 187 10 163 3.20e-39 132
MS.gene055591.t1 AT2G27580 39.306 173 86 3 15 187 10 163 3.20e-39 132
MS.gene055591.t1 AT4G12040 36.872 179 96 3 11 187 12 175 4.48e-39 132
MS.gene055591.t1 AT4G12040 36.872 179 96 3 11 187 12 175 4.48e-39 132
MS.gene055591.t1 AT1G12440 38.636 176 88 1 12 187 13 168 3.28e-38 129
MS.gene055591.t1 AT1G12440 38.636 176 88 1 12 187 13 168 3.28e-38 129
MS.gene055591.t1 AT1G12440 38.636 176 88 1 12 187 13 168 3.28e-38 129
MS.gene055591.t1 AT4G22820 36.994 173 94 2 15 187 19 176 4.98e-37 126
MS.gene055591.t1 AT4G22820 36.994 173 94 2 15 187 19 176 4.98e-37 126
MS.gene055591.t1 AT3G52800 41.489 188 86 5 4 187 3 170 9.72e-36 123
MS.gene055591.t1 AT3G52800 41.489 188 86 5 4 187 3 170 9.72e-36 123
MS.gene055591.t1 AT3G12630 37.349 166 67 5 23 187 31 160 4.38e-28 103
MS.gene055591.t1 AT4G14225 32.948 173 64 2 15 187 5 125 8.66e-26 96.3
MS.gene055591.t1 AT4G25380 28.409 176 65 4 18 187 10 130 3.56e-22 87.4

Find 50 sgRNAs with CRISPR-Local

Find 58 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAGGCTTAGAGATAGTTTC+AGG 0.302295 4.3:+38653299 None:intergenic
AGCTTCTCAGCCTTGACAAC+CGG 0.320370 4.3:+38653068 None:intergenic
TTCGGACCATCCTTCGGCTT+TGG 0.323240 4.3:+38653242 None:intergenic
ATGCACAATATAGGACCTTC+AGG 0.357857 4.3:+38653563 None:intergenic
GTTTCAGCATCATGTCTTTG+TGG 0.367335 4.3:+38653486 None:intergenic
CTTGACAACCGGGTTTGCTT+TGG 0.380130 4.3:+38653079 None:intergenic
TTGCAGTTGCTGCAACGTTT+CGG 0.398329 4.3:+38653224 None:intergenic
TCCCTTGGAAATATTATGAA+TGG 0.415286 4.3:-38653437 MS.gene055591:CDS
AGTTTCAGGTTCCACAGAAA+TGG 0.422622 4.3:+38653313 None:intergenic
CAAAGACATGATGCTGAAAC+AGG 0.424095 4.3:-38653483 MS.gene055591:CDS
AATAAGCGAGTTGGTTTGAC+AGG 0.426782 4.3:-38653203 MS.gene055591:CDS
CAACGTTTCGGACCATCCTT+CGG 0.445047 4.3:+38653236 None:intergenic
AACCGGGTTTGCTTTGGCTA+TGG 0.447808 4.3:+38653085 None:intergenic
CAGTTGTTGATGCACAATAT+AGG 0.453708 4.3:+38653554 None:intergenic
TTAGGTTCCACGGAAATGGC+TGG 0.467021 4.3:+38653359 None:intergenic
TCAGGTTCCACAGAAATGGC+TGG 0.480113 4.3:+38653317 None:intergenic
GCACTTAGAGCACATGTTCA+TGG 0.487125 4.3:+38653508 None:intergenic
CCCTTGGAAATATTATGAAT+GGG 0.495769 4.3:-38653436 MS.gene055591:CDS
CAGAAGTGCGGTAATCGAAC+GGG 0.502252 4.3:+38653117 None:intergenic
GCTTCTCAGCCTTGACAACC+GGG 0.508433 4.3:+38653069 None:intergenic
AAGGGATGATGCTGCAAGAT+GGG 0.510002 4.3:+38653454 None:intergenic
ACGCTGGCAGCAGTAACAAC+AGG 0.516439 4.3:+38653389 None:intergenic
CCCATTCATAATATTTCCAA+GGG 0.520390 4.3:+38653436 None:intergenic
ACCCATTCATAATATTTCCA+AGG 0.526039 4.3:+38653435 None:intergenic
CAAGGGATGATGCTGCAAGA+TGG 0.532070 4.3:+38653453 None:intergenic
AGCAACTGCAATAAGCGAGT+TGG 0.543723 4.3:-38653212 MS.gene055591:CDS
AGCGATGTACAGCACAGAAA+AGG 0.543830 4.3:+38653159 None:intergenic
CAGGTTCCACAGAAATGGCT+GGG 0.544362 4.3:+38653318 None:intergenic
CATCTTGCAGCATCATCCCT+TGG 0.544464 4.3:-38653452 MS.gene055591:CDS
CAAAGCAAACCCGGTTGTCA+AGG 0.546068 4.3:-38653078 MS.gene055591:CDS
TGCCATAGCCAAAGCAAACC+CGG 0.547092 4.3:-38653087 MS.gene055591:CDS
TTTATTTGGCTCAGGTTCAG+AGG 0.556901 4.3:-38653276 MS.gene055591:CDS
GCAGAAGTGCGGTAATCGAA+CGG 0.558885 4.3:+38653116 None:intergenic
TAGGTTCCACGGAAATGGCT+GGG 0.559679 4.3:+38653360 None:intergenic
TGCACAATATAGGACCTTCA+GGG 0.559940 4.3:+38653564 None:intergenic
CATGATGCTGAAACAGGAGC+AGG 0.593551 4.3:-38653477 MS.gene055591:CDS
ACAGGGTTTAATTGTCGATG+TGG 0.597602 4.3:-38653185 MS.gene055591:CDS
GGTGATCCAAAGCCGAAGGA+TGG 0.601061 4.3:-38653248 MS.gene055591:CDS
CGATATCCCAGCCATTTCTG+TGG 0.616564 4.3:-38653324 MS.gene055591:CDS
AATGGCTGGGATATCGACAC+TGG 0.621172 4.3:+38653331 None:intergenic
TGGCATCTCGTGCAGAAGTG+CGG 0.628982 4.3:+38653105 None:intergenic
GAGTGGTGATCCAAAGCCGA+AGG 0.630939 4.3:-38653252 MS.gene055591:CDS
GGATATCCCAGCCATTTCCG+TGG 0.638474 4.3:-38653366 MS.gene055591:CDS
GCACAATATAGGACCTTCAG+GGG 0.644318 4.3:+38653565 None:intergenic
ATAAGCGAGTTGGTTTGACA+GGG 0.651214 4.3:-38653202 MS.gene055591:CDS
AATGGCTGGGATATCCACGC+TGG 0.652769 4.3:+38653373 None:intergenic
GGCTCAGGTTCAGAGGAGAG+TGG 0.659270 4.3:-38653269 MS.gene055591:CDS
TGTTGTTACTGCTGCCAGCG+TGG 0.668169 4.3:-38653387 MS.gene055591:CDS
ATATTGTGCATCAACAACTG+TGG 0.675543 4.3:-38653551 MS.gene055591:CDS
CACAATATAGGACCTTCAGG+GGG 0.733275 4.3:+38653566 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATCTCTAAGCCTTTTTTATT+TGG - chr4.3:38653361-38653380 MS.gene055591:CDS 25.0%
ACCCATTCATAATATTTCCA+AGG + chr4.3:38653219-38653238 None:intergenic 30.0%
CCCATTCATAATATTTCCAA+GGG + chr4.3:38653218-38653237 None:intergenic 30.0%
CCCTTGGAAATATTATGAAT+GGG - chr4.3:38653215-38653234 MS.gene055591:CDS 30.0%
TCCCTTGGAAATATTATGAA+TGG - chr4.3:38653214-38653233 MS.gene055591:CDS 30.0%
! ATCAACAACTGTGGATTTTT+CGG - chr4.3:38653109-38653128 MS.gene055591:CDS 30.0%
AAAGGCTTAGAGATAGTTTC+AGG + chr4.3:38653355-38653374 None:intergenic 35.0%
ATATTGTGCATCAACAACTG+TGG - chr4.3:38653100-38653119 MS.gene055591:CDS 35.0%
CAGTTGTTGATGCACAATAT+AGG + chr4.3:38653100-38653119 None:intergenic 35.0%
GAACCTGAGCCAAATAAAAA+AGG + chr4.3:38653373-38653392 None:intergenic 35.0%
!!! AAGCCTTTTTTATTTGGCTC+AGG - chr4.3:38653367-38653386 MS.gene055591:CDS 35.0%
ACAGGGTTTAATTGTCGATG+TGG - chr4.3:38653466-38653485 MS.gene055591:CDS 40.0%
ATGCACAATATAGGACCTTC+AGG + chr4.3:38653091-38653110 None:intergenic 40.0%
CAAAGACATGATGCTGAAAC+AGG - chr4.3:38653168-38653187 MS.gene055591:CDS 40.0%
GTTTCAGCATCATGTCTTTG+TGG + chr4.3:38653168-38653187 None:intergenic 40.0%
TGCACAATATAGGACCTTCA+GGG + chr4.3:38653090-38653109 None:intergenic 40.0%
! ATATCGACACTGGCAGTTTT+AGG + chr4.3:38653313-38653332 None:intergenic 40.0%
! TTTATTTGGCTCAGGTTCAG+AGG - chr4.3:38653375-38653394 MS.gene055591:CDS 40.0%
!! AATAAGCGAGTTGGTTTGAC+AGG - chr4.3:38653448-38653467 MS.gene055591:CDS 40.0%
!! AGTTTCAGGTTCCACAGAAA+TGG + chr4.3:38653341-38653360 None:intergenic 40.0%
!! ATAAGCGAGTTGGTTTGACA+GGG - chr4.3:38653449-38653468 MS.gene055591:CDS 40.0%
!!! AGTTTTAGGTTCCACGGAAA+TGG + chr4.3:38653299-38653318 None:intergenic 40.0%
AAGGGATGATGCTGCAAGAT+GGG + chr4.3:38653200-38653219 None:intergenic 45.0%
AGCAACTGCAATAAGCGAGT+TGG - chr4.3:38653439-38653458 MS.gene055591:CDS 45.0%
AGCGATGTACAGCACAGAAA+AGG + chr4.3:38653495-38653514 None:intergenic 45.0%
CACAATATAGGACCTTCAGG+GGG + chr4.3:38653088-38653107 None:intergenic 45.0%
GCACAATATAGGACCTTCAG+GGG + chr4.3:38653089-38653108 None:intergenic 45.0%
GCACTTAGAGCACATGTTCA+TGG + chr4.3:38653146-38653165 None:intergenic 45.0%
TTGCAGTTGCTGCAACGTTT+CGG + chr4.3:38653430-38653449 None:intergenic 45.0%
!!! ACTGGCAGTTTTAGGTTCCA+CGG + chr4.3:38653305-38653324 None:intergenic 45.0%
AATGGCTGGGATATCGACAC+TGG + chr4.3:38653323-38653342 None:intergenic 50.0%
AGCTTCTCAGCCTTGACAAC+CGG + chr4.3:38653586-38653605 None:intergenic 50.0%
CAAAGCAAACCCGGTTGTCA+AGG - chr4.3:38653573-38653592 MS.gene055591:CDS 50.0%
CAACGTTTCGGACCATCCTT+CGG + chr4.3:38653418-38653437 None:intergenic 50.0%
CAAGGGATGATGCTGCAAGA+TGG + chr4.3:38653201-38653220 None:intergenic 50.0%
CAGGTTCCACAGAAATGGCT+GGG + chr4.3:38653336-38653355 None:intergenic 50.0%
CATCTTGCAGCATCATCCCT+TGG - chr4.3:38653199-38653218 MS.gene055591:CDS 50.0%
CATGATGCTGAAACAGGAGC+AGG - chr4.3:38653174-38653193 MS.gene055591:CDS 50.0%
CGATATCCCAGCCATTTCTG+TGG - chr4.3:38653327-38653346 MS.gene055591:CDS 50.0%
TAGGTTCCACGGAAATGGCT+GGG + chr4.3:38653294-38653313 None:intergenic 50.0%
TCAGGTTCCACAGAAATGGC+TGG + chr4.3:38653337-38653356 None:intergenic 50.0%
TGCCATAGCCAAAGCAAACC+CGG - chr4.3:38653564-38653583 MS.gene055591:CDS 50.0%
TTAGGTTCCACGGAAATGGC+TGG + chr4.3:38653295-38653314 None:intergenic 50.0%
! AACCGGGTTTGCTTTGGCTA+TGG + chr4.3:38653569-38653588 None:intergenic 50.0%
! CAGAAGTGCGGTAATCGAAC+GGG + chr4.3:38653537-38653556 None:intergenic 50.0%
! CTTGACAACCGGGTTTGCTT+TGG + chr4.3:38653575-38653594 None:intergenic 50.0%
! GCAGAAGTGCGGTAATCGAA+CGG + chr4.3:38653538-38653557 None:intergenic 50.0%
AATGGCTGGGATATCCACGC+TGG + chr4.3:38653281-38653300 None:intergenic 55.0%
ACGCTGGCAGCAGTAACAAC+AGG + chr4.3:38653265-38653284 None:intergenic 55.0%
GAATGTCAAGCTCCCCCTGA+AGG - chr4.3:38653073-38653092 MS.gene055591:CDS 55.0%
GAGTGGTGATCCAAAGCCGA+AGG - chr4.3:38653399-38653418 MS.gene055591:CDS 55.0%
GCTTCTCAGCCTTGACAACC+GGG + chr4.3:38653585-38653604 None:intergenic 55.0%
GGATATCCCAGCCATTTCCG+TGG - chr4.3:38653285-38653304 MS.gene055591:CDS 55.0%
GGTGATCCAAAGCCGAAGGA+TGG - chr4.3:38653403-38653422 MS.gene055591:CDS 55.0%
TGGCATCTCGTGCAGAAGTG+CGG + chr4.3:38653549-38653568 None:intergenic 55.0%
TGTTGTTACTGCTGCCAGCG+TGG - chr4.3:38653264-38653283 MS.gene055591:CDS 55.0%
TTCGGACCATCCTTCGGCTT+TGG + chr4.3:38653412-38653431 None:intergenic 55.0%
GGCTCAGGTTCAGAGGAGAG+TGG - chr4.3:38653382-38653401 MS.gene055591:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 38653055 38653618 38653055 ID=MS.gene055591
chr4.3 mRNA 38653055 38653618 38653055 ID=MS.gene055591.t1;Parent=MS.gene055591
chr4.3 exon 38653055 38653618 38653055 ID=MS.gene055591.t1.exon1;Parent=MS.gene055591.t1
chr4.3 CDS 38653055 38653618 38653055 ID=cds.MS.gene055591.t1;Parent=MS.gene055591.t1
Gene Sequence

>MS.gene055591

ATGGAACATGAAAAGACTGAATGTCAAGCTCCCCCTGAAGGTCCTATATTGTGCATCAACAACTGTGGATTTTTCGGAAGTGCAGCTACCATGAACATGTGCTCTAAGTGCCACAAAGACATGATGCTGAAACAGGAGCAGGCCCATCTTGCAGCATCATCCCTTGGAAATATTATGAATGGGTCTACAAGCAACACTGAAAAAGAACCTGTTGTTACTGCTGCCAGCGTGGATATCCCAGCCATTTCCGTGGAACCTAAAACTGCCAGTGTCGATATCCCAGCCATTTCTGTGGAACCTGAAACTATCTCTAAGCCTTTTTTATTTGGCTCAGGTTCAGAGGAGAGTGGTGATCCAAAGCCGAAGGATGGTCCGAAACGTTGCAGCAACTGCAATAAGCGAGTTGGTTTGACAGGGTTTAATTGTCGATGTGGCAACCTTTTCTGTGCTGTACATCGCTACTCAGACAAGCATGATTGCCCGTTCGATTACCGCACTTCTGCACGAGATGCCATAGCCAAAGCAAACCCGGTTGTCAAGGCTGAGAAGCTTGATAAGATCTGA

Protein sequence

>MS.gene055591.t1

MEHEKTECQAPPEGPILCINNCGFFGSAATMNMCSKCHKDMMLKQEQAHLAASSLGNIMNGSTSNTEKEPVVTAASVDIPAISVEPKTASVDIPAISVEPETISKPFLFGSGSEESGDPKPKDGPKRCSNCNKRVGLTGFNCRCGNLFCAVHRYSDKHDCPFDYRTSARDAIAKANPVVKAEKLDKI