AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene25264

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25264.t1 MTR_4g124910 95.257 253 12 0 1 253 1 253 1.37e-174 481
MS.gene25264.t1 MTR_2g043230 76.378 254 59 1 1 253 1 254 6.61e-142 398
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25264.t1 AT5G20720 75.294 255 59 2 1 253 1 253 1.20e-135 382
MS.gene25264.t1 AT5G20720 75.294 255 59 2 1 253 1 253 1.20e-135 382
MS.gene25264.t1 AT5G20720 75.294 255 59 2 1 253 1 253 1.20e-135 382
MS.gene25264.t1 AT5G20720 75.294 255 59 2 1 253 1 253 1.20e-135 382

Find 64 sgRNAs with CRISPR-Local

Find 162 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAGTCATTCCCTGCATATT+TGG 0.259970 8.2:-7908437 None:intergenic
AACTGTATTCCCGGGTGAAA+TGG 0.321202 8.2:-7908407 None:intergenic
CCTCGTAACAATGCTAGAAT+TGG 0.335567 8.2:+7906526 MS.gene25264:CDS
TTCCCGGGTGAAATGGATAA+TGG 0.363835 8.2:-7908400 None:intergenic
TTTAAGATCTTTGACCTCTT+CGG 0.402758 8.2:-7907664 None:intergenic
AGGAACCTTTCTTCCTTTGA+AGG 0.413360 8.2:+7906478 MS.gene25264:CDS
GGAGTACAAAACTGTATTCC+CGG 0.420138 8.2:-7908416 None:intergenic
TATCAAGACTCTGTCATTCA+AGG 0.421253 8.2:-7907688 None:intergenic
CCTTCGTCATCCACAGGCCC+TGG 0.444066 8.2:-7908367 None:intergenic
GGCTTTGATTGAGCAGTTGA+TGG 0.449739 8.2:-7907377 None:intergenic
CTGTTGCCTTCGTCATCCAC+AGG 0.452383 8.2:-7908373 None:intergenic
CAGGTGATAGCTGTTGGTCC+AGG 0.460455 8.2:+7908349 MS.gene25264:intron
GAATGACAGAGTCTTGATAA+AGG 0.473936 8.2:+7907692 MS.gene25264:CDS
TTGGAGACGGGAAGACAGTT+GGG 0.483437 8.2:+7907423 MS.gene25264:CDS
GCAATCCTTCAAAGGAAGAA+AGG 0.496887 8.2:-7906483 None:intergenic
GCCAATGGATGGCTTATCCT+TGG 0.505865 8.2:-7907926 None:intergenic
AATGACTTCAAAGGCAAAGA+TGG 0.508584 8.2:+7908451 MS.gene25264:CDS
GAGGTAGTTGCTGTTGGAGA+CGG 0.521962 8.2:+7907410 MS.gene25264:CDS
GTTGGAGACGGGAAGACAGT+TGG 0.523055 8.2:+7907422 MS.gene25264:CDS
TGCTGGTGGTTTGTTGCTCA+CGG 0.524264 8.2:+7907897 MS.gene25264:CDS
ATCAAGACTCTGTCATTCAA+GGG 0.529761 8.2:-7907687 None:intergenic
CTGCTCAATCAAAGCCTCAA+GGG 0.533192 8.2:+7907384 MS.gene25264:CDS
AGGTAGTTGCTGTTGGAGAC+GGG 0.539082 8.2:+7907411 MS.gene25264:CDS
GCCACTAGGTGATAGAGTAC+TGG 0.539283 8.2:+7907307 MS.gene25264:CDS
AAGCTTACCGTGCCAATGGA+TGG 0.542126 8.2:-7907937 None:intergenic
AGAAAAGCTTACCGTGCCAA+TGG 0.548046 8.2:-7907941 None:intergenic
GGAACTGAAGTGGAGTTCAA+TGG 0.549387 8.2:+7907591 MS.gene25264:CDS
AGCTGTTGGTCCAGGGCCTG+TGG 0.552111 8.2:+7908357 MS.gene25264:CDS
GGAAGTTCGCAATCCTTCAA+AGG 0.561777 8.2:-7906491 None:intergenic
GCGGCTTTAACAACAATAGA+CGG 0.565089 8.2:-7906571 None:intergenic
TTCAAAACATCTTATACTGA+AGG 0.568271 8.2:+7907614 MS.gene25264:CDS
CATACAACTGTCAAGCCACT+AGG 0.568378 8.2:+7907293 MS.gene25264:CDS
ACCAAGGATAAGCCATCCAT+TGG 0.568643 8.2:+7907925 MS.gene25264:CDS
TATGCAGGGAATGACTTCAA+AGG 0.569312 8.2:+7908442 MS.gene25264:CDS
TTTGAGAGCCTCAGATGTCA+TGG 0.571249 8.2:+7908489 MS.gene25264:CDS
GAGTACAAAACTGTATTCCC+GGG 0.572521 8.2:-7908415 None:intergenic
CTGAAGGATGATGACATCGT+TGG 0.572824 8.2:+7907630 MS.gene25264:CDS
TGCTCAATCAAAGCCTCAAG+GGG 0.578517 8.2:+7907385 MS.gene25264:CDS
AGGTGATAGCTGTTGGTCCA+GGG 0.579593 8.2:+7908350 MS.gene25264:intron
AAAGTATGCAGGAACTGAAG+TGG 0.581171 8.2:+7907581 MS.gene25264:CDS
ACTGCTCAATCAAAGCCTCA+AGG 0.582194 8.2:+7907383 MS.gene25264:CDS
GTTGTGTACTCAAAGTATGC+AGG 0.590902 8.2:+7907570 MS.gene25264:CDS
AAACCATTATCCATTTCACC+CGG 0.592297 8.2:+7908397 MS.gene25264:CDS
CGGCTTTAACAACAATAGAC+GGG 0.595648 8.2:-7906570 None:intergenic
GGATGCAGAAGAGAAAACTC+AGG 0.597980 8.2:+7907340 MS.gene25264:CDS
ACCAGTACTCTATCACCTAG+TGG 0.604909 8.2:-7907308 None:intergenic
CAAAGTGGACATTAGTGTGA+AGG 0.607471 8.2:+7907451 MS.gene25264:CDS
GAATTGGTATTCTTACACAA+AGG 0.614844 8.2:+7906542 MS.gene25264:CDS
CCAGGGCCTGTGGATGACGA+AGG 0.620963 8.2:+7908367 MS.gene25264:CDS
AAAATAGCCAAAATTAAGAG+AGG 0.622578 8.2:-7908516 None:intergenic
GTTGCTCACGGAAGCAACCA+AGG 0.626344 8.2:+7907909 MS.gene25264:CDS
GAGGATAGCCATGACATCTG+AGG 0.634831 8.2:-7908497 None:intergenic
CCAATTCTAGCATTGTTACG+AGG 0.641555 8.2:-7906526 None:intergenic
AACCATTATCCATTTCACCC+GGG 0.641591 8.2:+7908398 MS.gene25264:CDS
GTTACGAGGAAAATGAATTG+CGG 0.642882 8.2:-7906512 None:intergenic
GGATAAGCCATCCATTGGCA+CGG 0.647992 8.2:+7907930 MS.gene25264:CDS
CTGCTTCATCGATTTCTACG+AGG 0.656045 8.2:+7906458 MS.gene25264:CDS
TGGTATTCTTGAAACCGAAG+AGG 0.662324 8.2:+7907650 MS.gene25264:CDS
GCTGCAGGTTGCAGAAGCTG+AGG 0.680718 8.2:+7907867 MS.gene25264:intron
GATGCAGAAGAGAAAACTCA+GGG 0.684794 8.2:+7907341 MS.gene25264:CDS
ATGTAACAGGTGATAGCTGT+TGG 0.691284 8.2:+7908343 MS.gene25264:intron
GACAGTTGGGAAGAACAAAG+TGG 0.692016 8.2:+7907436 MS.gene25264:CDS
GCAGAAGAGAAAACTCAGGG+TGG 0.728959 8.2:+7907344 MS.gene25264:CDS
GCTCAATCAAAGCCTCAAGG+GGG 0.730531 8.2:+7907386 MS.gene25264:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAATAAAAATAATACCT+TGG - chr8.2:7906611-7906630 None:intergenic 10.0%
!! AAAAATAAAAATAATACCTT+GGG - chr8.2:7906610-7906629 None:intergenic 10.0%
!!! AAGGTATTATTTTTATTTTT+TGG + chr8.2:7906610-7906629 MS.gene25264:intron 10.0%
!!! AGGTATTATTTTTATTTTTT+GGG + chr8.2:7906611-7906630 MS.gene25264:intron 10.0%
!!! TTTTCAAAAAATTAAAACAA+TGG + chr8.2:7908080-7908099 MS.gene25264:intron 10.0%
!! AAAATAAAAATAATACCTTG+GGG - chr8.2:7906609-7906628 None:intergenic 15.0%
!! CGAAAAATATTCATAAATAT+TGG - chr8.2:7907088-7907107 None:intergenic 15.0%
!! GTTAAGACAAATAATTAAAT+GGG - chr8.2:7907774-7907793 None:intergenic 15.0%
!! AAATAAAAATAATACCTTGG+GGG - chr8.2:7906608-7906627 None:intergenic 20.0%
!! AAGTATACTGTTTCAAAAAA+AGG - chr8.2:7907972-7907991 None:intergenic 20.0%
!! ACAAGATGAAATTACTAATA+AGG - chr8.2:7907271-7907290 None:intergenic 20.0%
!! CAAAAAATTAAAACAATGGA+TGG + chr8.2:7908084-7908103 MS.gene25264:intron 20.0%
!! GAAAAATATTCAAAAATGGT+GGG - chr8.2:7907062-7907081 None:intergenic 20.0%
!! GGTTAAGACAAATAATTAAA+TGG - chr8.2:7907775-7907794 None:intergenic 20.0%
!! GTCTGAAAAATATTCAAAAA+TGG - chr8.2:7907066-7907085 None:intergenic 20.0%
!! TGAAAAATATTCAAAAATGG+TGG - chr8.2:7907063-7907082 None:intergenic 20.0%
!! TTCAGACCAATAAAAAAAAA+GGG + chr8.2:7906987-7907006 MS.gene25264:intron 20.0%
!! TTTCAGACCAATAAAAAAAA+AGG + chr8.2:7906986-7907005 MS.gene25264:intron 20.0%
!!! TACATTTTCTCTTATACTAA+GGG - chr8.2:7906879-7906898 None:intergenic 20.0%
!!! TATTTTTATTTTTTGGGTCA+TGG + chr8.2:7906617-7906636 MS.gene25264:intron 20.0%
! TAACACCTTAATGCAAAATA+TGG - chr8.2:7907796-7907815 None:intergenic 25.0%
! TGTAATGTATTTCTCTTTAC+AGG + chr8.2:7907536-7907555 MS.gene25264:intron 25.0%
! TTCAAAACATCTTATACTGA+AGG + chr8.2:7907614-7907633 MS.gene25264:CDS 25.0%
!! ACATTTTCTCTTATACTAAG+GGG - chr8.2:7906878-7906897 None:intergenic 25.0%
!! CTTAACCATATTTTGCATTA+AGG + chr8.2:7907788-7907807 MS.gene25264:intron 25.0%
!! GTACATTTTCTCTTATACTA+AGG - chr8.2:7906880-7906899 None:intergenic 25.0%
!! TTTATTTATTGCTTTAGCTC+AGG + chr8.2:7908226-7908245 MS.gene25264:intron 25.0%
AAACACTAGAAATAATCCCA+TGG - chr8.2:7907213-7907232 None:intergenic 30.0%
AACACTAGAAATAATCCCAT+GGG - chr8.2:7907212-7907231 None:intergenic 30.0%
AATAAAAAAAAAGGGCAACC+CGG + chr8.2:7906995-7907014 MS.gene25264:intron 30.0%
AATTAAAACAATGGATGGTC+TGG + chr8.2:7908089-7908108 MS.gene25264:intron 30.0%
AGAATTGCAAGAATGTGATT+TGG + chr8.2:7908053-7908072 MS.gene25264:intron 30.0%
CCAAAATAAAAGAAAGACCA+AGG - chr8.2:7907511-7907530 None:intergenic 30.0%
GAATGGTTATTTACATACCT+AGG - chr8.2:7908311-7908330 None:intergenic 30.0%
GAATTGCAAGAATGTGATTT+GGG + chr8.2:7908054-7908073 MS.gene25264:intron 30.0%
GAATTGGTATTCTTACACAA+AGG + chr8.2:7906542-7906561 MS.gene25264:CDS 30.0%
GCAATGTTATATCGTATGAA+GGG + chr8.2:7908021-7908040 MS.gene25264:intron 30.0%
GTTGTGTTTATAAGCTATCT+TGG + chr8.2:7906730-7906749 MS.gene25264:intron 30.0%
TTGAAAGAGCTTATAGCTTA+TGG - chr8.2:7906797-7906816 None:intergenic 30.0%
! AATAACCATTCTACTCCTTT+TGG + chr8.2:7908320-7908339 MS.gene25264:intron 30.0%
! CATTAAGGTGTTACGTTTAA+AGG + chr8.2:7907803-7907822 MS.gene25264:intron 30.0%
! TGGATTTTCTTCATTATCTG+CGG + chr8.2:7906637-7906656 MS.gene25264:intron 30.0%
! TTTAAGATCTTTGACCTCTT+CGG - chr8.2:7907667-7907686 None:intergenic 30.0%
! TTTTGTACTCCAAATATGCA+GGG + chr8.2:7908428-7908447 MS.gene25264:CDS 30.0%
!! CCTTGGTCTTTCTTTTATTT+TGG + chr8.2:7907508-7907527 MS.gene25264:intron 30.0%
!! TAGAGTACTGGTAAAAATCA+AGG + chr8.2:7907319-7907338 MS.gene25264:CDS 30.0%
!!! TTGTTTTGATCGAGTAGTTT+TGG + chr8.2:7908284-7908303 MS.gene25264:intron 30.0%
AAACCATTATCCATTTCACC+CGG + chr8.2:7908397-7908416 MS.gene25264:CDS 35.0%
AAAGAAAGACCAAGGCTTAA+AGG - chr8.2:7907503-7907522 None:intergenic 35.0%
AACACATTCTACCGTCTTAA+GGG - chr8.2:7908181-7908200 None:intergenic 35.0%
AATGACTTCAAAGGCAAAGA+TGG + chr8.2:7908451-7908470 MS.gene25264:CDS 35.0%
ACCTGCAGCATCATAATTAA+AGG - chr8.2:7907856-7907875 None:intergenic 35.0%
ACTAGCATTATCCCTTAAGA+CGG + chr8.2:7908167-7908186 MS.gene25264:intron 35.0%
ATCAAGACTCTGTCATTCAA+GGG - chr8.2:7907690-7907709 None:intergenic 35.0%
ATCTCATAATGCACTTGAGT+GGG - chr8.2:7906823-7906842 None:intergenic 35.0%
ATGTATTTCTCTTTACAGGC+AGG + chr8.2:7907540-7907559 MS.gene25264:intron 35.0%
GAATGACAGAGTCTTGATAA+AGG + chr8.2:7907692-7907711 MS.gene25264:CDS 35.0%
GGCAATGTTATATCGTATGA+AGG + chr8.2:7908020-7908039 MS.gene25264:intron 35.0%
GTTACGAGGAAAATGAATTG+CGG - chr8.2:7906515-7906534 None:intergenic 35.0%
TAAAGGATTCCATAAGCTAG+TGG - chr8.2:7907486-7907505 None:intergenic 35.0%
TACATCCAAAAGGAGTAGAA+TGG - chr8.2:7908328-7908347 None:intergenic 35.0%
TATCAAGACTCTGTCATTCA+AGG - chr8.2:7907691-7907710 None:intergenic 35.0%
TATCACCTGTTACATCCAAA+AGG - chr8.2:7908338-7908357 None:intergenic 35.0%
TATCTCATAATGCACTTGAG+TGG - chr8.2:7906824-7906843 None:intergenic 35.0%
TGTTATATCGTATGAAGGGA+AGG + chr8.2:7908025-7908044 MS.gene25264:intron 35.0%
TTATGGAATCCTTTAAGCCT+TGG + chr8.2:7907491-7907510 MS.gene25264:intron 35.0%
! AACACCACTCTTCTAAAAGA+GGG + chr8.2:7907999-7908018 MS.gene25264:intron 35.0%
! GTTTTGTACTCCAAATATGC+AGG + chr8.2:7908427-7908446 MS.gene25264:CDS 35.0%
! TAACACCACTCTTCTAAAAG+AGG + chr8.2:7907998-7908017 MS.gene25264:intron 35.0%
!!! GGGTTGCCCTTTTTTTTTAT+TGG - chr8.2:7906996-7907015 None:intergenic 35.0%
AAAGTATGCAGGAACTGAAG+TGG + chr8.2:7907581-7907600 MS.gene25264:CDS 40.0%
AACCATTATCCATTTCACCC+GGG + chr8.2:7908398-7908417 MS.gene25264:CDS 40.0%
ACAGAGTCTTGATAAAGGTG+TGG + chr8.2:7907697-7907716 MS.gene25264:intron 40.0%
AGAAATAATCCCATGGGACA+GGG - chr8.2:7907206-7907225 None:intergenic 40.0%
AGGAACCTTTCTTCCTTTGA+AGG + chr8.2:7906478-7906497 MS.gene25264:CDS 40.0%
ATGTAACAGGTGATAGCTGT+TGG + chr8.2:7908343-7908362 MS.gene25264:intron 40.0%
CAAAGTGGACATTAGTGTGA+AGG + chr8.2:7907451-7907470 MS.gene25264:CDS 40.0%
CAACACATTCTACCGTCTTA+AGG - chr8.2:7908182-7908201 None:intergenic 40.0%
CCAATTCTAGCATTGTTACG+AGG - chr8.2:7906529-7906548 None:intergenic 40.0%
CCTCGTAACAATGCTAGAAT+TGG + chr8.2:7906526-7906545 MS.gene25264:CDS 40.0%
GAAGTCATTCCCTGCATATT+TGG - chr8.2:7908440-7908459 None:intergenic 40.0%
GAGTACAAAACTGTATTCCC+GGG - chr8.2:7908418-7908437 None:intergenic 40.0%
GATGCAGAAGAGAAAACTCA+GGG + chr8.2:7907341-7907360 MS.gene25264:CDS 40.0%
GCAATCCTTCAAAGGAAGAA+AGG - chr8.2:7906486-7906505 None:intergenic 40.0%
GCCTTTAATTATGATGCTGC+AGG + chr8.2:7907852-7907871 MS.gene25264:intron 40.0%
GGAGTACAAAACTGTATTCC+CGG - chr8.2:7908419-7908438 None:intergenic 40.0%
GTTGTGTACTCAAAGTATGC+AGG + chr8.2:7907570-7907589 MS.gene25264:CDS 40.0%
TAGAAATAATCCCATGGGAC+AGG - chr8.2:7907207-7907226 None:intergenic 40.0%
TATGCAGGGAATGACTTCAA+AGG + chr8.2:7908442-7908461 MS.gene25264:CDS 40.0%
TGGTATTCTTGAAACCGAAG+AGG + chr8.2:7907650-7907669 MS.gene25264:CDS 40.0%
! ATTGCTTTAGCTCAGGTCAT+TGG + chr8.2:7908233-7908252 MS.gene25264:intron 40.0%
! CGGCTTTAACAACAATAGAC+GGG - chr8.2:7906573-7906592 None:intergenic 40.0%
! CTACTCCTTTTGGATGTAAC+AGG + chr8.2:7908330-7908349 MS.gene25264:intron 40.0%
! GCGGCTTTAACAACAATAGA+CGG - chr8.2:7906574-7906593 None:intergenic 40.0%
! TTTAGCTCAGGTCATTGGTT+TGG + chr8.2:7908238-7908257 MS.gene25264:intron 40.0%
!! ATTGCCCTCTTTTAGAAGAG+TGG - chr8.2:7908006-7908025 None:intergenic 40.0%
AACTGTATTCCCGGGTGAAA+TGG - chr8.2:7908410-7908429 None:intergenic 45.0%
ACCAAGGATAAGCCATCCAT+TGG + chr8.2:7907925-7907944 MS.gene25264:CDS 45.0%
ACCAGTACTCTATCACCTAG+TGG - chr8.2:7907311-7907330 None:intergenic 45.0%
ACTGCTCAATCAAAGCCTCA+AGG + chr8.2:7907383-7907402 MS.gene25264:CDS 45.0%
AGAAAAGCTTACCGTGCCAA+TGG - chr8.2:7907944-7907963 None:intergenic 45.0%
ATACATAAGCCCTGTCCCAT+GGG + chr8.2:7907194-7907213 MS.gene25264:intron 45.0%
CATACAACTGTCAAGCCACT+AGG + chr8.2:7907293-7907312 MS.gene25264:CDS 45.0%
CTACAGCGAGAGATCAAAGT+AGG + chr8.2:7906928-7906947 MS.gene25264:intron 45.0%
CTGAAGGATGATGACATCGT+TGG + chr8.2:7907630-7907649 MS.gene25264:CDS 45.0%
CTGCTCAATCAAAGCCTCAA+GGG + chr8.2:7907384-7907403 MS.gene25264:CDS 45.0%
GAAGCTGAGGAAAAAACTGC+TGG + chr8.2:7907880-7907899 MS.gene25264:CDS 45.0%
GACAGTTGGGAAGAACAAAG+TGG + chr8.2:7907436-7907455 MS.gene25264:CDS 45.0%
GGAACTGAAGTGGAGTTCAA+TGG + chr8.2:7907591-7907610 MS.gene25264:CDS 45.0%
GGAAGTTCGCAATCCTTCAA+AGG - chr8.2:7906494-7906513 None:intergenic 45.0%
GGATGCAGAAGAGAAAACTC+AGG + chr8.2:7907340-7907359 MS.gene25264:CDS 45.0%
GGGATAATGCTAGTTGCTAC+AGG - chr8.2:7908161-7908180 None:intergenic 45.0%
TATACATAAGCCCTGTCCCA+TGG + chr8.2:7907193-7907212 MS.gene25264:intron 45.0%
TGCTCAATCAAAGCCTCAAG+GGG + chr8.2:7907385-7907404 MS.gene25264:CDS 45.0%
TTCCCGGGTGAAATGGATAA+TGG - chr8.2:7908403-7908422 None:intergenic 45.0%
TTTGAGAGCCTCAGATGTCA+TGG + chr8.2:7908489-7908508 MS.gene25264:CDS 45.0%
! GGCTTTGATTGAGCAGTTGA+TGG - chr8.2:7907380-7907399 None:intergenic 45.0%
! TGAGTGATGCCACTAGCTTA+TGG + chr8.2:7907474-7907493 MS.gene25264:intron 45.0%
!! CTGCTTCATCGATTTCTACG+AGG + chr8.2:7906458-7906477 MS.gene25264:CDS 45.0%
!!! GATCGAGTAGTTTTGGTCCT+AGG + chr8.2:7908291-7908310 MS.gene25264:intron 45.0%
AAGCTTACCGTGCCAATGGA+TGG - chr8.2:7907940-7907959 None:intergenic 50.0%
AGGTGATAGCTGTTGGTCCA+GGG + chr8.2:7908350-7908369 MS.gene25264:intron 50.0%
GAGGATAGCCATGACATCTG+AGG - chr8.2:7908500-7908519 None:intergenic 50.0%
GCAGAAGAGAAAACTCAGGG+TGG + chr8.2:7907344-7907363 MS.gene25264:CDS 50.0%
GCCAATGGATGGCTTATCCT+TGG - chr8.2:7907929-7907948 None:intergenic 50.0%
GCCACTAGGTGATAGAGTAC+TGG + chr8.2:7907307-7907326 MS.gene25264:CDS 50.0%
GCTCAATCAAAGCCTCAAGG+GGG + chr8.2:7907386-7907405 MS.gene25264:CDS 50.0%
GCTGAGGAAAAAACTGCTGG+TGG + chr8.2:7907883-7907902 MS.gene25264:CDS 50.0%
GGATAAGCCATCCATTGGCA+CGG + chr8.2:7907930-7907949 MS.gene25264:CDS 50.0%
GGGCAGATCATCAAGACAAC+TGG - chr8.2:7907754-7907773 None:intergenic 50.0%
TACCTTGGGGGTAACAACAG+TGG - chr8.2:7906596-7906615 None:intergenic 50.0%
TTGGAGACGGGAAGACAGTT+GGG + chr8.2:7907423-7907442 MS.gene25264:CDS 50.0%
! AGGTAGTTGCTGTTGGAGAC+GGG + chr8.2:7907411-7907430 MS.gene25264:CDS 50.0%
! GAGGTAGTTGCTGTTGGAGA+CGG + chr8.2:7907410-7907429 MS.gene25264:CDS 50.0%
!! TGCTGGTGGTTTGTTGCTCA+CGG + chr8.2:7907897-7907916 MS.gene25264:CDS 50.0%
ACTAAAGCTCCCGCATACGC+GGG + chr8.2:7907021-7907040 MS.gene25264:intron 55.0%
ATCAAAGCCTCAAGGGGGTG+AGG + chr8.2:7907391-7907410 MS.gene25264:CDS 55.0%
CACTAAAGCTCCCGCATACG+CGG + chr8.2:7907020-7907039 MS.gene25264:intron 55.0%
CAGGTGATAGCTGTTGGTCC+AGG + chr8.2:7908349-7908368 MS.gene25264:intron 55.0%
CTGTTGCCTTCGTCATCCAC+AGG - chr8.2:7908376-7908395 None:intergenic 55.0%
CTTGGGGGTAACAACAGTGG+CGG - chr8.2:7906593-7906612 None:intergenic 55.0%
GTTGCTCACGGAAGCAACCA+AGG + chr8.2:7907909-7907928 MS.gene25264:CDS 55.0%
GTTGGAGACGGGAAGACAGT+TGG + chr8.2:7907422-7907441 MS.gene25264:CDS 55.0%
! ATGCGGGAGCTTTAGTGCAC+CGG - chr8.2:7907017-7907036 None:intergenic 55.0%
CGCCACTGTTGTTACCCCCA+AGG + chr8.2:7906591-7906610 MS.gene25264:CDS 60.0%
CTAAAGCTCCCGCATACGCG+GGG + chr8.2:7907022-7907041 MS.gene25264:intron 60.0%
GCAACTACCTCACCCCCTTG+AGG - chr8.2:7907401-7907420 None:intergenic 60.0%
GCTGCAGGTTGCAGAAGCTG+AGG + chr8.2:7907867-7907886 MS.gene25264:intron 60.0%
GGGGGTGAGGTAGTTGCTGT+TGG + chr8.2:7907404-7907423 MS.gene25264:CDS 60.0%
! AAATGGTGGGACCCCCTTCC+CGG - chr8.2:7907049-7907068 None:intergenic 60.0%
! TGCGGGAGCTTTAGTGCACC+GGG - chr8.2:7907016-7907035 None:intergenic 60.0%
AGCTGTTGGTCCAGGGCCTG+TGG + chr8.2:7908357-7908376 MS.gene25264:CDS 65.0%
CCAGGGCCTGTGGATGACGA+AGG + chr8.2:7908367-7908386 MS.gene25264:CDS 65.0%
CCTTCGTCATCCACAGGCCC+TGG - chr8.2:7908370-7908389 None:intergenic 65.0%
ATACGCGGGGTCCGGGAAGG+GGG + chr8.2:7907035-7907054 MS.gene25264:intron 70.0%
CATACGCGGGGTCCGGGAAG+GGG + chr8.2:7907034-7907053 MS.gene25264:intron 70.0%
CTTCCCGGACCCCGCGTATG+CGG - chr8.2:7907034-7907053 None:intergenic 70.0%
GCATACGCGGGGTCCGGGAA+GGG + chr8.2:7907033-7907052 MS.gene25264:intron 70.0%
TTCCCGGACCCCGCGTATGC+GGG - chr8.2:7907033-7907052 None:intergenic 70.0%
CGCATACGCGGGGTCCGGGA+AGG + chr8.2:7907032-7907051 MS.gene25264:intron 75.0%
CTCCCGCATACGCGGGGTCC+GGG + chr8.2:7907028-7907047 MS.gene25264:intron 75.0%
GCTCCCGCATACGCGGGGTC+CGG + chr8.2:7907027-7907046 MS.gene25264:intron 75.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 7906439 7908525 7906439 ID=MS.gene25264
chr8.2 mRNA 7906439 7908525 7906439 ID=MS.gene25264.t1;Parent=MS.gene25264
chr8.2 exon 7906439 7906612 7906439 ID=MS.gene25264.t1.exon1;Parent=MS.gene25264.t1
chr8.2 CDS 7906439 7906612 7906439 ID=cds.MS.gene25264.t1;Parent=MS.gene25264.t1
chr8.2 exon 7907293 7907472 7907293 ID=MS.gene25264.t1.exon2;Parent=MS.gene25264.t1
chr8.2 CDS 7907293 7907472 7907293 ID=cds.MS.gene25264.t1;Parent=MS.gene25264.t1
chr8.2 exon 7907558 7907713 7907558 ID=MS.gene25264.t1.exon3;Parent=MS.gene25264.t1
chr8.2 CDS 7907558 7907713 7907558 ID=cds.MS.gene25264.t1;Parent=MS.gene25264.t1
chr8.2 exon 7907874 7907951 7907874 ID=MS.gene25264.t1.exon4;Parent=MS.gene25264.t1
chr8.2 CDS 7907874 7907951 7907874 ID=cds.MS.gene25264.t1;Parent=MS.gene25264.t1
chr8.2 exon 7908352 7908525 7908352 ID=MS.gene25264.t1.exon5;Parent=MS.gene25264.t1
chr8.2 CDS 7908352 7908525 7908352 ID=cds.MS.gene25264.t1;Parent=MS.gene25264.t1
Gene Sequence

>MS.gene25264

ATGGCGACTACTCAGCTTACTGCTTCATCGATTTCTACGAGGAACCTTTCTTCCTTTGAAGGATTGCGAACTTCCGCAATTCATTTTCCTCGTAACAATGCTAGAATTGGTATTCTTACACAAAGGTCGTTCCCGTCTATTGTTGTTAAAGCCGCCACTGTTGTTACCCCCAAGGTATTATTTTTATTTTTTGGGTCATGGATTTTCTTCATTATCTGCGGCAAAATTATTCTTTTCAGTAGTATATTATGATAAAATAGAGTTAAATTGTTTTTGTATATGCTGCTATAAGTTGTGTTTATAAGCTATCTTGGAGAACTTGCGAAATTAAGTTGAGAAACACTTATGAAAAGTGCCATAAGCTATAAGCTCTTTCAAACACCCACTCAAGTGCATTATGAGATAGTTATCACACAAGTGCATTATCTTACGAGTGCCCCTTAGTATAAGAGAAAATGTACAATTTGTGAGTAGATAGAGAAAGCGAAGCTACAGCGAGAGATCAAAGTAGGAGTTAGAAAGAGTGAAAAATTCATTTGCAACTAAATTTCAGACCAATAAAAAAAAAGGGCAACCCGGTGCACTAAAGCTCCCGCATACGCGGGGTCCGGGAAGGGGGTCCCACCATTTTTGAATATTTTTCAGACCAATATTTATGAATATTTTTCGTACAGAAGAACATTATCTTTTTATTTCGTAAGCGAAATAGCAGACTATTGATATACATTTCATCTTGAATTAACATGTTCATTGATATACATAAGCCCTGTCCCATGGGATTATTTCTAGTGTTTTTACTTCTACATTTTACAATTCAATGCTGCATGCGCCTTATTAGTAATTTCATCTTGTAGCATACAACTGTCAAGCCACTAGGTGATAGAGTACTGGTAAAAATCAAGGATGCAGAAGAGAAAACTCAGGGTGGAATTTTGCTGCCATCAACTGCTCAATCAAAGCCTCAAGGGGGTGAGGTAGTTGCTGTTGGAGACGGGAAGACAGTTGGGAAGAACAAAGTGGACATTAGTGTGAAGGTGAGTGATGCCACTAGCTTATGGAATCCTTTAAGCCTTGGTCTTTCTTTTATTTTGGATTTATGTAATGTATTTCTCTTTACAGGCAGGTGCACAAGTTGTGTACTCAAAGTATGCAGGAACTGAAGTGGAGTTCAATGGTTCAAAACATCTTATACTGAAGGATGATGACATCGTTGGTATTCTTGAAACCGAAGAGGTCAAAGATCTTAAACCCTTGAATGACAGAGTCTTGATAAAGGTGTGGAACTTTTCTGAGATTTGATTATTTCGATGCTCCAGTTGTCTTGATGATCTGCCCATTTAATTATTTGTCTTAACCATATTTTGCATTAAGGTGTTACGTTTAAAGGCAATATTATATCTGAATATTATACATGCCTTTAATTATGATGCTGCAGGTTGCAGAAGCTGAGGAAAAAACTGCTGGTGGTTTGTTGCTCACGGAAGCAACCAAGGATAAGCCATCCATTGGCACGGTAAGCTTTTCTATTCTCCTTTTTTTGAAACAGTATACTTAAAAAGTAACACCACTCTTCTAAAAGAGGGCAATGTTATATCGTATGAAGGGAAGGTCACAAGAATTGCAAGAATGTGATTTGGGCTGTTTTCAAAAAATTAAAACAATGGATGGTCTGGTGAAAATGAACGCATTAGATACTGTCGTGCATTTAATTTTTGTGTTCCTGTAGCAACTAGCATTATCCCTTAAGACGGTAGAATGTGTTGATGACAGTTTAACTGATGCTTATTTTTATTTATTGCTTTAGCTCAGGTCATTGGTTTGGTTCTTACTTGCTTAGCTGTAACATTGTTTTGATCGAGTAGTTTTGGTCCTAGGTATGTAAATAACCATTCTACTCCTTTTGGATGTAACAGGTGATAGCTGTTGGTCCAGGGCCTGTGGATGACGAAGGCAACAGAAAACCATTATCCATTTCACCCGGGAATACAGTTTTGTACTCCAAATATGCAGGGAATGACTTCAAAGGCAAAGATGGTTCTGACTATATTGCTTTGAGAGCCTCAGATGTCATGGCTATCCTCTCTTAA
Protein sequence

>MS.gene25264.t1

MATTQLTASSISTRNLSSFEGLRTSAIHFPRNNARIGILTQRSFPSIVVKAATVVTPKHTTVKPLGDRVLVKIKDAEEKTQGGILLPSTAQSKPQGGEVVAVGDGKTVGKNKVDISVKAGAQVVYSKYAGTEVEFNGSKHLILKDDDIVGILETEEVKDLKPLNDRVLIKVAEAEEKTAGGLLLTEATKDKPSIGTVIAVGPGPVDDEGNRKPLSISPGNTVLYSKYAGNDFKGKDGSDYIALRASDVMAILS