AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene25966

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25966.t1 MTR_3g077750 98.810 336 4 0 1 336 1 336 0.0 690
MS.gene25966.t1 MTR_3g091820 34.320 338 153 17 17 324 5 303 2.42e-40 144
MS.gene25966.t1 MTR_4g461080 55.039 129 42 3 39 159 9 129 4.43e-36 132
MS.gene25966.t1 MTR_2g093220 54.331 127 45 4 38 155 19 141 1.21e-35 131
MS.gene25966.t1 MTR_8g027295 52.800 125 44 4 45 158 10 130 1.96e-35 130
MS.gene25966.t1 MTR_4g063780 58.491 106 37 3 44 142 55 160 2.96e-34 128
MS.gene25966.t1 MTR_2g096740 72.603 73 18 1 50 122 51 121 8.85e-34 126
MS.gene25966.t1 MTR_2g096740 72.603 73 18 1 50 122 66 136 9.47e-34 126
MS.gene25966.t1 MTR_1g077600 53.125 128 46 5 41 163 25 143 1.58e-33 125
MS.gene25966.t1 MTR_1g077600 53.125 128 46 5 41 163 25 143 1.73e-33 125
MS.gene25966.t1 MTR_8g479350 66.197 71 24 0 61 131 68 138 3.74e-31 120
MS.gene25966.t1 MTR_4g022370 69.333 75 20 2 44 115 62 136 1.27e-30 119
MS.gene25966.t1 MTR_7g059400 60.870 92 29 3 27 115 43 130 5.02e-30 117
MS.gene25966.t1 MTR_7g024670 56.122 98 27 3 34 115 34 131 1.04e-29 117
MS.gene25966.t1 MTR_8g068210 67.164 67 22 0 50 116 21 87 1.08e-29 116
MS.gene25966.t1 MTR_3g090430 71.875 64 18 0 52 115 42 105 1.09e-29 116
MS.gene25966.t1 MTR_2g014060 77.049 61 14 0 52 112 16 76 1.72e-29 115
MS.gene25966.t1 MTR_4g088580 81.818 55 10 0 62 116 56 110 2.33e-29 116
MS.gene25966.t1 MTR_8g079060 55.319 94 35 2 52 141 16 106 1.44e-28 110
MS.gene25966.t1 MTR_5g031440 78.571 56 12 0 56 111 38 93 1.57e-28 113
MS.gene25966.t1 MTR_1g056810 71.212 66 19 0 59 124 10 75 3.04e-28 111
MS.gene25966.t1 MTR_8g015840 74.603 63 16 0 56 118 15 77 8.44e-28 108
MS.gene25966.t1 MTR_4g109980 84.314 51 8 0 61 111 40 90 7.92e-27 108
MS.gene25966.t1 MTR_2g014170 84.000 50 8 0 60 109 44 93 5.20e-26 105
MS.gene25966.t1 MTR_2g059540 82.353 51 9 0 59 109 29 79 8.76e-26 102
MS.gene25966.t1 MTR_3g435480 66.667 69 22 1 50 118 126 193 1.39e-25 107
MS.gene25966.t1 MTR_2g013370 47.458 118 42 5 1 116 1 100 2.64e-25 103
MS.gene25966.t1 MTR_4g089095 76.786 56 12 1 61 116 16 70 3.04e-25 102
MS.gene25966.t1 MTR_4g089095 76.786 56 12 1 61 116 50 104 3.10e-25 103
MS.gene25966.t1 MTR_7g010950 43.697 119 59 2 25 136 93 210 4.97e-24 102
MS.gene25966.t1 MTR_4g082060 60.870 69 26 1 50 118 112 179 5.73e-24 102
MS.gene25966.t1 MTR_6g012450 44.545 110 54 1 30 132 98 207 7.30e-24 102
MS.gene25966.t1 MTR_7g086780 42.342 111 58 2 20 130 64 168 1.47e-23 100
MS.gene25966.t1 MTR_6g027450 42.478 113 57 4 7 119 43 147 3.12e-23 98.6
MS.gene25966.t1 MTR_8g044220 53.333 75 34 1 41 115 99 172 6.25e-23 99.0
MS.gene25966.t1 MTR_6g027460 55.714 70 30 1 50 119 86 154 9.31e-23 97.8
MS.gene25966.t1 MTR_5g041420 41.600 125 55 3 12 118 71 195 3.65e-22 95.5
MS.gene25966.t1 MTR_2g016030 66.667 54 18 0 61 114 47 100 4.52e-22 91.3
MS.gene25966.t1 MTR_5g041530 63.793 58 21 0 57 114 136 193 1.86e-20 91.7
MS.gene25966.t1 MTR_5g041400 45.161 93 47 1 26 114 80 172 4.14e-20 90.1
MS.gene25966.t1 MTR_5g041380 68.519 54 17 0 61 114 127 180 8.89e-20 89.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25966.t1 AT5G62940 42.748 393 145 23 1 334 1 372 1.23e-55 186
MS.gene25966.t1 AT5G60200 66.292 89 27 3 33 121 30 115 2.44e-35 129
MS.gene25966.t1 AT1G51700 47.656 128 54 2 56 170 28 155 6.94e-33 121
MS.gene25966.t1 AT2G28510 56.364 110 35 2 63 160 50 158 5.09e-32 121
MS.gene25966.t1 AT3G45610 43.373 166 68 7 54 212 32 178 2.35e-31 118
MS.gene25966.t1 AT4G24060 50.000 122 46 2 50 156 42 163 2.66e-31 120
MS.gene25966.t1 AT5G02460 70.667 75 19 2 44 115 75 149 5.04e-31 121
MS.gene25966.t1 AT3G55370 65.000 80 25 2 43 119 55 134 7.04e-31 119
MS.gene25966.t1 AT3G55370 65.000 80 25 2 43 119 55 134 8.62e-31 119
MS.gene25966.t1 AT3G55370 65.000 80 25 2 43 119 100 179 1.27e-30 119
MS.gene25966.t1 AT2G37590 64.474 76 24 1 44 116 69 144 1.70e-30 118
MS.gene25966.t1 AT1G07640 69.444 72 19 1 47 115 4 75 6.19e-30 115
MS.gene25966.t1 AT5G60850 66.234 77 20 1 50 120 34 110 6.56e-30 116
MS.gene25966.t1 AT1G07640 69.444 72 19 1 47 115 60 131 1.06e-29 116
MS.gene25966.t1 AT1G07640 69.444 72 19 1 47 115 68 139 1.19e-29 116
MS.gene25966.t1 AT1G64620 52.672 131 47 3 45 164 33 159 1.73e-29 116
MS.gene25966.t1 AT3G61850 65.753 73 23 1 46 118 49 119 3.42e-29 114
MS.gene25966.t1 AT3G61850 65.753 73 23 1 46 118 49 119 3.42e-29 114
MS.gene25966.t1 AT5G65590 62.963 81 25 1 35 115 20 95 3.75e-29 114
MS.gene25966.t1 AT3G61850 65.753 73 23 1 46 118 61 131 3.81e-29 114
MS.gene25966.t1 AT3G61850 65.753 73 23 1 46 118 49 119 5.75e-29 114
MS.gene25966.t1 AT3G61850 65.753 73 23 1 46 118 61 131 5.97e-29 114
MS.gene25966.t1 AT3G52440 68.182 66 21 0 58 123 22 87 1.01e-28 111
MS.gene25966.t1 AT1G28310 82.143 56 10 0 61 116 27 82 1.36e-28 112
MS.gene25966.t1 AT3G52440 68.182 66 21 0 58 123 41 106 1.98e-28 111
MS.gene25966.t1 AT1G28310 82.143 56 10 0 61 116 51 106 2.11e-28 112
MS.gene25966.t1 AT1G21340 69.444 72 19 1 63 134 40 108 2.95e-28 110
MS.gene25966.t1 AT3G21270 54.717 106 42 3 61 164 29 130 4.79e-28 108
MS.gene25966.t1 AT4G21050 59.551 89 33 2 54 142 19 104 5.79e-28 108
MS.gene25966.t1 AT2G28810 65.152 66 22 1 47 112 64 128 5.83e-28 111
MS.gene25966.t1 AT2G28810 65.152 66 22 1 47 112 81 145 1.03e-27 111
MS.gene25966.t1 AT4G00940 53.398 103 31 2 63 151 70 169 8.74e-27 107
MS.gene25966.t1 AT4G00940 53.398 103 31 2 63 151 70 169 8.74e-27 107
MS.gene25966.t1 AT4G00940 53.398 103 31 2 63 151 70 169 8.74e-27 107
MS.gene25966.t1 AT2G46590 55.682 88 36 2 22 108 30 115 2.54e-26 107
MS.gene25966.t1 AT4G38000 60.256 78 30 1 41 117 21 98 2.57e-26 105
MS.gene25966.t1 AT2G46590 55.682 88 36 2 22 108 42 127 2.92e-26 107
MS.gene25966.t1 AT3G50410 75.439 57 14 0 61 117 30 86 7.15e-26 104
MS.gene25966.t1 AT5G66940 70.968 62 18 0 61 122 32 93 1.04e-25 103
MS.gene25966.t1 AT1G47655 73.684 57 13 1 55 109 21 77 5.82e-25 100
MS.gene25966.t1 AT3G47500 63.235 68 24 1 51 118 101 167 4.78e-24 102
MS.gene25966.t1 AT5G62430 46.087 115 48 3 50 164 44 144 5.92e-24 100
MS.gene25966.t1 AT5G39660 63.768 69 24 1 50 118 128 195 9.76e-24 101
MS.gene25966.t1 AT5G39660 63.768 69 24 1 50 118 128 195 9.76e-24 101
MS.gene25966.t1 AT5G39660 63.768 69 24 1 50 118 128 195 9.76e-24 101
MS.gene25966.t1 AT1G69570 64.516 62 21 1 53 114 125 185 3.84e-23 99.4
MS.gene25966.t1 AT1G29160 60.000 70 26 1 45 114 48 115 4.41e-23 94.7
MS.gene25966.t1 AT2G34140 47.253 91 36 1 24 114 33 111 1.44e-22 93.2
MS.gene25966.t1 AT4G21040 57.971 69 29 0 63 131 27 95 4.52e-22 93.6
MS.gene25966.t1 AT1G26790 53.333 75 35 0 58 132 108 182 8.48e-22 95.1
MS.gene25966.t1 AT4G21080 56.000 75 30 1 63 134 27 101 4.18e-21 91.3
MS.gene25966.t1 AT4G21030 55.556 63 25 1 50 112 13 72 6.45e-15 72.8

Find 89 sgRNAs with CRISPR-Local

Find 111 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACTTGTTCTCCATGAAATT+AGG 0.146876 3.2:+68733632 None:intergenic
AATCACACCTTGGACATTTA+AGG 0.155190 3.2:+68733966 None:intergenic
AGTAAGTTAGAAGGCATAAT+TGG 0.168612 3.2:-68733557 MS.gene25966:CDS
TCTCACCTAAGTAACTTATT+TGG 0.188613 3.2:-68733677 MS.gene25966:CDS
AAAGAAAGTTGAAGATCTTT+AGG 0.198842 3.2:+68733716 None:intergenic
TAGCAATGGTGCTTACTTAA+TGG 0.209633 3.2:-68733375 MS.gene25966:CDS
CATTCAAGTGACAATAGTTT+AGG 0.231938 3.2:+68733275 None:intergenic
ACTTGTTCTCCATGAAATTA+GGG 0.276978 3.2:+68733633 None:intergenic
GATATGATGAATGGACAAAA+TGG 0.284919 3.2:-68733455 MS.gene25966:CDS
TTTGGAGCTAATGGAGCATT+AGG 0.302981 3.2:-68733659 MS.gene25966:CDS
TGATCATATGAATATGAATA+TGG 0.304551 3.2:-68733495 MS.gene25966:CDS
AGTAACTTATTTGGAGCTAA+TGG 0.308114 3.2:-68733668 MS.gene25966:CDS
TTCTCTTCACGTCTGCAATA+TGG 0.315298 3.2:-68734625 MS.gene25966:CDS
AAGAAAGTTGAAGATCTTTA+GGG 0.315430 3.2:+68733717 None:intergenic
ATTCAAGTGACAATAGTTTA+GGG 0.332848 3.2:+68733276 None:intergenic
ATCACACCTTGGACATTTAA+GGG 0.338610 3.2:+68733967 None:intergenic
CTGGGAAAGGTCAGTCATTT+GGG 0.359835 3.2:-68733226 MS.gene25966:CDS
GCTGGGAAAGGTCAGTCATT+TGG 0.360092 3.2:-68733227 MS.gene25966:CDS
TTTCTACAGCCTCCACCAAC+TGG 0.361161 3.2:+68733833 None:intergenic
ATGAATATGGGTGTTGGGAT+TGG 0.368536 3.2:-68733482 MS.gene25966:CDS
CTTTCCATCATGAAGTCAAT+AGG 0.377139 3.2:+68733578 None:intergenic
GCAAGACTTGTAGAAGGTAT+TGG 0.393470 3.2:-68733883 MS.gene25966:CDS
GTTGTTGTAGTAACAGAATT+TGG 0.394528 3.2:+68733934 None:intergenic
ATTAGGAAACCCTAATTTCA+TGG 0.397565 3.2:-68733642 MS.gene25966:CDS
GATCAAGGTTGCTCTGATGC+TGG 0.405144 3.2:-68733245 MS.gene25966:CDS
AGTGACAATAGTTTAGGGTT+TGG 0.405871 3.2:+68733281 None:intergenic
TTTGGTGGAATGTCATTTGA+TGG 0.425628 3.2:-68733407 MS.gene25966:CDS
TCCTCATCATTAGCATCATA+TGG 0.425855 3.2:+68733329 None:intergenic
GTAAGTTAGAAGGCATAATT+GGG 0.426365 3.2:-68733556 MS.gene25966:CDS
TAGCTCCAAATAAGTTACTT+AGG 0.442973 3.2:+68733672 None:intergenic
AGGTGAGAGAATTGCACATC+AGG 0.445358 3.2:+68733692 None:intergenic
ATCTGCAGATCATTGGTTGC+AGG 0.446436 3.2:-68734598 MS.gene25966:intron
TCTTAGCCTCCTCTCTATCA+TGG 0.458753 3.2:+68734006 None:intergenic
AGTCATTTGGGTATGGATCA+TGG 0.466736 3.2:-68733214 MS.gene25966:CDS
ATGATGGAAAGTAAGTTAGA+AGG 0.467778 3.2:-68733566 MS.gene25966:CDS
TGGAATCATCTGCAGATCAT+TGG 0.469488 3.2:-68734605 MS.gene25966:CDS
CTAAACTATTGTCACTTGAA+TGG 0.473946 3.2:-68733274 MS.gene25966:CDS
GAGCTTAGCCGTTGATCAAA+GGG 0.477519 3.2:+68733153 None:intergenic
TGTAGAAGGTATTGGACAAA+AGG 0.479247 3.2:-68733875 MS.gene25966:CDS
AGTGGTATGATGAATGGTTA+TGG 0.480178 3.2:-68733191 MS.gene25966:CDS
AATATGGGTGTTGGGATTGG+AGG 0.481246 3.2:-68733479 MS.gene25966:CDS
GGCACAATTCACGATGAGTC+AGG 0.484101 3.2:-68734085 MS.gene25966:CDS
GATTCATCTTCACCAATGTC+TGG 0.489933 3.2:-68734058 MS.gene25966:CDS
GAGAACAAGTATAGCAATGT+TGG 0.493849 3.2:-68733620 MS.gene25966:CDS
CTCCTCTCTATCATGGATGA+TGG 0.495201 3.2:+68734013 None:intergenic
ACTTCTGCAAGACTTGTAGA+AGG 0.495701 3.2:-68733889 MS.gene25966:CDS
ATCAAGGTTGCTCTGATGCT+GGG 0.498182 3.2:-68733244 MS.gene25966:CDS
AGAGCTTAGCCGTTGATCAA+AGG 0.500555 3.2:+68733152 None:intergenic
ATGAATATGAATATGGGTGT+TGG 0.503188 3.2:-68733488 MS.gene25966:CDS
GTGACATGCTAACATGTTCA+AGG 0.508689 3.2:-68734036 MS.gene25966:CDS
TGAATATGAATATGGGTGTT+GGG 0.514370 3.2:-68733487 MS.gene25966:CDS
ATCATGAAGTCAATAGGCCT+AGG 0.516098 3.2:+68733584 None:intergenic
AAAGGTCAGTCATTTGGGTA+TGG 0.536234 3.2:-68733221 MS.gene25966:CDS
CTTGAATGGCAACAAGATCA+AGG 0.543433 3.2:-68733260 MS.gene25966:CDS
TTCTACAGCCTCCACCAACT+GGG 0.549436 3.2:+68733834 None:intergenic
GGTTGCTCTGATGCTGGGAA+AGG 0.557290 3.2:-68733239 MS.gene25966:CDS
CAAGTCTTGCAGAAGTACCT+TGG 0.558008 3.2:+68733896 None:intergenic
CTTAGGAACATCCCAGTTGG+TGG 0.561107 3.2:-68733845 MS.gene25966:CDS
ACCTATCCCTTAAATGTCCA+AGG 0.564253 3.2:-68733973 MS.gene25966:CDS
ACAACAAACCCTTTGATCAA+CGG 0.565654 3.2:-68733161 MS.gene25966:CDS
ATATGATGAATGGACAAAAT+GGG 0.566744 3.2:-68733454 MS.gene25966:CDS
ATTGGAGGAGATATGATGAA+TGG 0.570060 3.2:-68733464 MS.gene25966:CDS
GATCATATGAATATGAATAT+GGG 0.580380 3.2:-68733494 MS.gene25966:CDS
AGGAACATCCCAGTTGGTGG+AGG 0.580870 3.2:-68733842 MS.gene25966:CDS
TAGGCCTATTGACTTCATGA+TGG 0.581168 3.2:-68733582 MS.gene25966:CDS
TCATGGAGTGGTATGATGAA+TGG 0.582721 3.2:-68733197 MS.gene25966:CDS
ACCTTGGACATTTAAGGGAT+AGG 0.582910 3.2:+68733972 None:intergenic
GGACAAAAGGAGGAACACTT+AGG 0.590540 3.2:-68733862 MS.gene25966:CDS
AATTCACGATGAGTCAGGAA+TGG 0.596611 3.2:-68734080 MS.gene25966:CDS
AGGAAGGTACATGATGTGGT+TGG 0.607867 3.2:+68733750 None:intergenic
GGTGGAATGTCATTTGATGG+AGG 0.611132 3.2:-68733404 MS.gene25966:CDS
TCATGAAGTCAATAGGCCTA+GGG 0.612063 3.2:+68733585 None:intergenic
GGCCATCATCCATGATAGAG+AGG 0.614387 3.2:-68734015 MS.gene25966:CDS
GATGGAGGAAACAATAGCAA+TGG 0.614470 3.2:-68733389 MS.gene25966:CDS
GGTGAGAGAATTGCACATCA+GGG 0.615522 3.2:+68733693 None:intergenic
TTGGCATGCTTGAAAACCCT+AGG 0.618350 3.2:-68733601 MS.gene25966:CDS
AGAAGGTATTGGACAAAAGG+AGG 0.623390 3.2:-68733872 MS.gene25966:CDS
GTTAGCATGTCACCAGACAT+TGG 0.624948 3.2:+68734046 None:intergenic
TGGTAGGAAGGTACATGATG+TGG 0.632743 3.2:+68733746 None:intergenic
CATTTAAGGGATAGGTCATG+TGG 0.639862 3.2:+68733980 None:intergenic
GTTGATCAAAGGGTTTGTTG+TGG 0.647747 3.2:+68733163 None:intergenic
TTTGGGTATGGATCATGGAG+TGG 0.650428 3.2:-68733209 MS.gene25966:CDS
ACACTTAGGAACATCCCAGT+TGG 0.662546 3.2:-68733848 MS.gene25966:CDS
ACTACAGTCTCTCTCAGCCA+AGG 0.663924 3.2:-68733913 MS.gene25966:CDS
GTGTGAGTTGAATCACACCT+TGG 0.665322 3.2:+68733956 None:intergenic
TCCATATGATGCTAATGATG+AGG 0.665364 3.2:-68733330 MS.gene25966:CDS
AATGCATGATGAAAATTCTG+TGG 0.671096 3.2:+68733428 None:intergenic
CATCATCCATGATAGAGAGG+AGG 0.679330 3.2:-68734012 MS.gene25966:CDS
TTAAGGGATAGGTCATGTGG+TGG 0.699015 3.2:+68733983 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGAAATTTTGATAATTATTT+TGG - chr3.2:68734269-68734288 MS.gene25966:intron 10.0%
!! AACATGATAAACAAAATGAA+AGG + chr3.2:68733252-68733271 None:intergenic 20.0%
!! AAGCTAAGAAAAACAAAATT+AGG - chr3.2:68733534-68733553 MS.gene25966:CDS 20.0%
!! ACATGCATTAATCAAATTAA+TGG - chr3.2:68733286-68733305 MS.gene25966:CDS 20.0%
!! AGCTAAGAAAAACAAAATTA+GGG - chr3.2:68733535-68733554 MS.gene25966:CDS 20.0%
!! GATCATATGAATATGAATAT+GGG - chr3.2:68734299-68734318 MS.gene25966:intron 20.0%
!! TAAACAAAATGAAAGGAAAA+TGG + chr3.2:68733245-68733264 None:intergenic 20.0%
!! TGATCATATGAATATGAATA+TGG - chr3.2:68734298-68734317 MS.gene25966:intron 20.0%
!!! TCTTTTATACAAAATTGTCA+GGG - chr3.2:68733687-68733706 MS.gene25966:CDS 20.0%
!!! TTCTTTTATACAAAATTGTC+AGG - chr3.2:68733686-68733705 MS.gene25966:CDS 20.0%
! AAAGAAAGTTGAAGATCTTT+AGG + chr3.2:68734080-68734099 None:intergenic 25.0%
! AAATTAATGGTGTAGTAGTT+TGG - chr3.2:68733299-68733318 MS.gene25966:CDS 25.0%
! AAGAAAGTTGAAGATCTTTA+GGG + chr3.2:68734079-68734098 None:intergenic 25.0%
! ATATGATGAATGGACAAAAT+GGG - chr3.2:68734339-68734358 MS.gene25966:intron 25.0%
! ATTCAAGTGACAATAGTTTA+GGG + chr3.2:68734520-68734539 None:intergenic 25.0%
! CAAAAAATCAAATGATCACT+TGG - chr3.2:68734001-68734020 MS.gene25966:CDS 25.0%
!! GAAAAGAGAATTTTATGCTT+AGG - chr3.2:68733321-68733340 MS.gene25966:CDS 25.0%
!!! AATGTGTTAATTTGTCTTGT+TGG + chr3.2:68733626-68733645 None:intergenic 25.0%
!!! CAAGTGATCATTTGATTTTT+TGG + chr3.2:68734003-68734022 None:intergenic 25.0%
!!! TGGAATTGGATTATTTTACA+TGG + chr3.2:68733606-68733625 None:intergenic 25.0%
!!! TTAATTTGTCTTGTTGGAAT+TGG + chr3.2:68733620-68733639 None:intergenic 25.0%
!!! TTCACTTTTAATCATGAGAT+CGG + chr3.2:68733489-68733508 None:intergenic 25.0%
AAAATGGTATACAATGTGCA+TGG + chr3.2:68733229-68733248 None:intergenic 30.0%
AATGCATGATGAAAATTCTG+TGG + chr3.2:68734368-68734387 None:intergenic 30.0%
ACTTGTTCTCCATGAAATTA+GGG + chr3.2:68734163-68734182 None:intergenic 30.0%
ATGAATATGAATATGGGTGT+TGG - chr3.2:68734305-68734324 MS.gene25966:intron 30.0%
ATTAGGAAACCCTAATTTCA+TGG - chr3.2:68734151-68734170 MS.gene25966:intron 30.0%
CATGATCATTTGAATTGCTA+TGG - chr3.2:68733396-68733415 MS.gene25966:CDS 30.0%
CATTCAAGTGACAATAGTTT+AGG + chr3.2:68734521-68734540 None:intergenic 30.0%
GATATGATGAATGGACAAAA+TGG - chr3.2:68734338-68734357 MS.gene25966:intron 30.0%
GATCTCATGATTAAAAGTGA+AGG - chr3.2:68733488-68733507 MS.gene25966:CDS 30.0%
GTTGTTGTAGTAACAGAATT+TGG + chr3.2:68733862-68733881 None:intergenic 30.0%
TACTTGTTCTCCATGAAATT+AGG + chr3.2:68734164-68734183 None:intergenic 30.0%
TAGCTCCAAATAAGTTACTT+AGG + chr3.2:68734124-68734143 None:intergenic 30.0%
TCTCACCTAAGTAACTTATT+TGG - chr3.2:68734116-68734135 MS.gene25966:intron 30.0%
TGAATATGAATATGGGTGTT+GGG - chr3.2:68734306-68734325 MS.gene25966:intron 30.0%
! AATTTTCATCATGCATTTGG+TGG - chr3.2:68734371-68734390 MS.gene25966:intron 30.0%
! AGTAACTTATTTGGAGCTAA+TGG - chr3.2:68734125-68734144 MS.gene25966:intron 30.0%
! AGTAAGTTAGAAGGCATAAT+TGG - chr3.2:68734236-68734255 MS.gene25966:intron 30.0%
! ATGATGGAAAGTAAGTTAGA+AGG - chr3.2:68734227-68734246 MS.gene25966:intron 30.0%
! CAGAATTTTCATCATGCATT+TGG - chr3.2:68734368-68734387 MS.gene25966:intron 30.0%
! CTAAACTATTGTCACTTGAA+TGG - chr3.2:68734519-68734538 MS.gene25966:intron 30.0%
! GTAAGTTAGAAGGCATAATT+GGG - chr3.2:68734237-68734256 MS.gene25966:intron 30.0%
AATCACACCTTGGACATTTA+AGG + chr3.2:68733830-68733849 None:intergenic 35.0%
ACAACAAACCCTTTGATCAA+CGG - chr3.2:68734632-68734651 MS.gene25966:CDS 35.0%
AGTGGTATGATGAATGGTTA+TGG - chr3.2:68734602-68734621 MS.gene25966:CDS 35.0%
ATCACACCTTGGACATTTAA+GGG + chr3.2:68733829-68733848 None:intergenic 35.0%
ATTGGAGGAGATATGATGAA+TGG - chr3.2:68734329-68734348 MS.gene25966:intron 35.0%
CTTTCCATCATGAAGTCAAT+AGG + chr3.2:68734218-68734237 None:intergenic 35.0%
GAGAACAAGTATAGCAATGT+TGG - chr3.2:68734173-68734192 MS.gene25966:intron 35.0%
TAGCAATGGTGCTTACTTAA+TGG - chr3.2:68734418-68734437 MS.gene25966:intron 35.0%
TCCATATGATGCTAATGATG+AGG - chr3.2:68734463-68734482 MS.gene25966:intron 35.0%
TCCTCATCATTAGCATCATA+TGG + chr3.2:68734467-68734486 None:intergenic 35.0%
! AGTGACAATAGTTTAGGGTT+TGG + chr3.2:68734515-68734534 None:intergenic 35.0%
! TTTGGTGGAATGTCATTTGA+TGG - chr3.2:68734386-68734405 MS.gene25966:intron 35.0%
!! TGTAGAAGGTATTGGACAAA+AGG - chr3.2:68733918-68733937 MS.gene25966:CDS 35.0%
!!! ATCTTTAGGGTTTTGATGGT+AGG + chr3.2:68734066-68734085 None:intergenic 35.0%
!!! GAAGATCTTTAGGGTTTTGA+TGG + chr3.2:68734070-68734089 None:intergenic 35.0%
AAAGGTCAGTCATTTGGGTA+TGG - chr3.2:68734572-68734591 MS.gene25966:intron 40.0%
AATTCACGATGAGTCAGGAA+TGG - chr3.2:68733713-68733732 MS.gene25966:CDS 40.0%
ACCTATCCCTTAAATGTCCA+AGG - chr3.2:68733820-68733839 MS.gene25966:CDS 40.0%
ACCTTGGACATTTAAGGGAT+AGG + chr3.2:68733824-68733843 None:intergenic 40.0%
ACTTCTGCAAGACTTGTAGA+AGG - chr3.2:68733904-68733923 MS.gene25966:CDS 40.0%
AGTCATTTGGGTATGGATCA+TGG - chr3.2:68734579-68734598 MS.gene25966:intron 40.0%
ATCATGAAGTCAATAGGCCT+AGG + chr3.2:68734212-68734231 None:intergenic 40.0%
CATTTAAGGGATAGGTCATG+TGG + chr3.2:68733816-68733835 None:intergenic 40.0%
CTTGAATGGCAACAAGATCA+AGG - chr3.2:68734533-68734552 MS.gene25966:intron 40.0%
GATGGAGGAAACAATAGCAA+TGG - chr3.2:68734404-68734423 MS.gene25966:intron 40.0%
GATTCATCTTCACCAATGTC+TGG - chr3.2:68733735-68733754 MS.gene25966:CDS 40.0%
GTGACATGCTAACATGTTCA+AGG - chr3.2:68733757-68733776 MS.gene25966:CDS 40.0%
TCATGAAGTCAATAGGCCTA+GGG + chr3.2:68734211-68734230 None:intergenic 40.0%
TCATGGAGTGGTATGATGAA+TGG - chr3.2:68734596-68734615 MS.gene25966:intron 40.0%
TGGAATCATCTGCAGATCAT+TGG - chr3.2:68733188-68733207 MS.gene25966:CDS 40.0%
TTCTCTTCACGTCTGCAATA+TGG - chr3.2:68733168-68733187 MS.gene25966:CDS 40.0%
! GCAAGACTTGTAGAAGGTAT+TGG - chr3.2:68733910-68733929 MS.gene25966:CDS 40.0%
! TTTGGAGCTAATGGAGCATT+AGG - chr3.2:68734134-68734153 MS.gene25966:intron 40.0%
!! AGAAGGTATTGGACAAAAGG+AGG - chr3.2:68733921-68733940 MS.gene25966:CDS 40.0%
!! ATGAATATGGGTGTTGGGAT+TGG - chr3.2:68734311-68734330 MS.gene25966:intron 40.0%
!! GTTGATCAAAGGGTTTGTTG+TGG + chr3.2:68734633-68734652 None:intergenic 40.0%
!! TAGGCCTATTGACTTCATGA+TGG - chr3.2:68734211-68734230 MS.gene25966:intron 40.0%
!! TTAGGGTTTTGATGGTAGGA+AGG + chr3.2:68734062-68734081 None:intergenic 40.0%
ACACTTAGGAACATCCCAGT+TGG - chr3.2:68733945-68733964 MS.gene25966:CDS 45.0%
AGGTGAGAGAATTGCACATC+AGG + chr3.2:68734104-68734123 None:intergenic 45.0%
ATCAAGGTTGCTCTGATGCT+GGG - chr3.2:68734549-68734568 MS.gene25966:intron 45.0%
CATCATCCATGATAGAGAGG+AGG - chr3.2:68733781-68733800 MS.gene25966:CDS 45.0%
CTCCTCTCTATCATGGATGA+TGG + chr3.2:68733783-68733802 None:intergenic 45.0%
CTGGGAAAGGTCAGTCATTT+GGG - chr3.2:68734567-68734586 MS.gene25966:intron 45.0%
GGACAAAAGGAGGAACACTT+AGG - chr3.2:68733931-68733950 MS.gene25966:CDS 45.0%
GGTGAGAGAATTGCACATCA+GGG + chr3.2:68734103-68734122 None:intergenic 45.0%
GTTAGCATGTCACCAGACAT+TGG + chr3.2:68733750-68733769 None:intergenic 45.0%
TCTTAGCCTCCTCTCTATCA+TGG + chr3.2:68733790-68733809 None:intergenic 45.0%
TTAAGGGATAGGTCATGTGG+TGG + chr3.2:68733813-68733832 None:intergenic 45.0%
TTGGCATGCTTGAAAACCCT+AGG - chr3.2:68734192-68734211 MS.gene25966:intron 45.0%
TTTGGGTATGGATCATGGAG+TGG - chr3.2:68734584-68734603 MS.gene25966:intron 45.0%
! AGGAAGGTACATGATGTGGT+TGG + chr3.2:68734046-68734065 None:intergenic 45.0%
! ATCTGCAGATCATTGGTTGC+AGG - chr3.2:68733195-68733214 MS.gene25966:CDS 45.0%
! GGTGGAATGTCATTTGATGG+AGG - chr3.2:68734389-68734408 MS.gene25966:intron 45.0%
! GTGTGAGTTGAATCACACCT+TGG + chr3.2:68733840-68733859 None:intergenic 45.0%
! TGGTAGGAAGGTACATGATG+TGG + chr3.2:68734050-68734069 None:intergenic 45.0%
!! AATATGGGTGTTGGGATTGG+AGG - chr3.2:68734314-68734333 MS.gene25966:intron 45.0%
!! CAAGTCTTGCAGAAGTACCT+TGG + chr3.2:68733900-68733919 None:intergenic 45.0%
ACTACAGTCTCTCTCAGCCA+AGG - chr3.2:68733880-68733899 MS.gene25966:CDS 50.0%
CTTAGGAACATCCCAGTTGG+TGG - chr3.2:68733948-68733967 MS.gene25966:CDS 50.0%
GATCAAGGTTGCTCTGATGC+TGG - chr3.2:68734548-68734567 MS.gene25966:intron 50.0%
GCTGGGAAAGGTCAGTCATT+TGG - chr3.2:68734566-68734585 MS.gene25966:intron 50.0%
GGCACAATTCACGATGAGTC+AGG - chr3.2:68733708-68733727 MS.gene25966:CDS 50.0%
GGCCATCATCCATGATAGAG+AGG - chr3.2:68733778-68733797 MS.gene25966:CDS 50.0%
TTCTACAGCCTCCACCAACT+GGG + chr3.2:68733962-68733981 None:intergenic 50.0%
TTTCTACAGCCTCCACCAAC+TGG + chr3.2:68733963-68733982 None:intergenic 50.0%
GGTTGCTCTGATGCTGGGAA+AGG - chr3.2:68734554-68734573 MS.gene25966:intron 55.0%
!! AGGAACATCCCAGTTGGTGG+AGG - chr3.2:68733951-68733970 MS.gene25966:CDS 55.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 68733157 68734658 68733157 ID=MS.gene25966
chr3.2 mRNA 68733157 68734658 68733157 ID=MS.gene25966.t1;Parent=MS.gene25966
chr3.2 exon 68734599 68734658 68734599 ID=MS.gene25966.t1.exon1;Parent=MS.gene25966.t1
chr3.2 CDS 68734599 68734658 68734599 ID=cds.MS.gene25966.t1;Parent=MS.gene25966.t1
chr3.2 exon 68733157 68734107 68733157 ID=MS.gene25966.t1.exon2;Parent=MS.gene25966.t1
chr3.2 CDS 68733157 68734107 68733157 ID=cds.MS.gene25966.t1;Parent=MS.gene25966.t1
Gene Sequence

>MS.gene25966

ATGGGTTTATCTTCTCTTCACGTCTGCAATATGGAATCATCTGCAGATCATTGGTTGCAGGTATATATACCATGCACATTGTATACCATTTTCCTTTCATTTTGTTTATCATGTTCACATATCACTAACACATGCATTAATCAAATTAATGGTGTAGTAGTTTGGAAAAGAGAATTTTATGCTTAGGTTTATGCTTTGTAAATAACTATGTTTTGTACATTAATTAGCTTAATTAGATTCATGATCATTTGAATTGCTATGGAATTTAATTAAAAGTGCTTTGTATAGCTTCTTCAAAAAAAAGTGCTTTGTATAGTATATGTTGAACTCCGATCTCATGATTAAAAGTGAAGGAGAACGAAAAATAAAAGAAGAGAAAGCTAAGAAAAACAAAATTAGGGAGAAGCTAGCTAGAAAGAGAACAAAAAGAATGAAAAAGAAAAAGACCATGTAAAATAATCCAATTCCAACAAGACAAATTAACACATTTTTCACTTTTACTTTTATCATAAATATATTAACTTCTAGTTTCTTTTATACAAAATTGTCAGGGCACAATTCACGATGAGTCAGGAATGGATTCATCTTCACCAATGTCTGGTGACATGCTAACATGTTCAAGGCCATCATCCATGATAGAGAGGAGGCTAAGACCACCACATGACCTATCCCTTAAATGTCCAAGGTGTGATTCAACTCACACCAAATTCTGTTACTACAACAACTACAGTCTCTCTCAGCCAAGGTACTTCTGCAAGACTTGTAGAAGGTATTGGACAAAAGGAGGAACACTTAGGAACATCCCAGTTGGTGGAGGCTGTAGAAAAAACAAAAAAGTTTCTTCCAAAAAATCAAATGATCACTTGGCTAATAATAATATTCAAAACCAACCACATCATGTACCTTCCTACCATCAAAACCCTAAAGATCTTCAACTTTCTTTCCCTGATGTGCAATTCTCTCACCTAAGTAACTTATTTGGAGCTAATGGAGCATTAGGAAACCCTAATTTCATGGAGAACAAGTATAGCAATGTTGGCATGCTTGAAAACCCTAGGCCTATTGACTTCATGATGGAAAGTAAGTTAGAAGGCATAATTGGGAGTAGTTCTAGAAATTTTGATAATTATTTTGGAAATAGTGATCATATGAATATGAATATGGGTGTTGGGATTGGAGGAGATATGATGAATGGACAAAATGGGTTGCCACAGAATTTTCATCATGCATTTGGTGGAATGTCATTTGATGGAGGAAACAATAGCAATGGTGCTTACTTAATGGATTCTTGTCAAAGACTTATGCTTCCATATGATGCTAATGATGAGGATCATAATGCTTCAATTGATGTGAAGCCAAACCCTAAACTATTGTCACTTGAATGGCAACAAGATCAAGGTTGCTCTGATGCTGGGAAAGGTCAGTCATTTGGGTATGGATCATGGAGTGGTATGATGAATGGTTATGGATCTTCCACAACAAACCCTTTGATCAACGGCTAA
Protein sequence

>MS.gene25966.t1

MGLSSLHVCNMESSADHWLQGTIHDESGMDSSSPMSGDMLTCSRPSSMIERRLRPPHDLSLKCPRCDSTHTKFCYYNNYSLSQPRYFCKTCRRYWTKGGTLRNIPVGGGCRKNKKVSSKKSNDHLANNNIQNQPHHVPSYHQNPKDLQLSFPDVQFSHLSNLFGANGALGNPNFMENKYSNVGMLENPRPIDFMMESKLEGIIGSSSRNFDNYFGNSDHMNMNMGVGIGGDMMNGQNGLPQNFHHAFGGMSFDGGNNSNGAYLMDSCQRLMLPYDANDEDHNASIDVKPNPKLLSLEWQQDQGCSDAGKGQSFGYGSWSGMMNGYGSSTTNPLING