Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05315.t1 | XP_013452927.1 | 90.5 | 275 | 16 | 1 | 207 | 471 | 1 | 275 | 1.60E-129 | 473 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05315.t1 | P15170 | 69.8 | 192 | 58 | 0 | 284 | 475 | 71 | 262 | 1.2e-77 | 292.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05315.t1 | A0A072UM85 | 90.5 | 275 | 16 | 1 | 207 | 471 | 1 | 275 | 1.2e-129 | 473.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene05315 | 0.819401 | 1.18E-52 | -1.69E-46 |
| MS.gene050084 | MS.gene05315 | 0.835257 | 1.86E-56 | -1.69E-46 |
| MS.gene050085 | MS.gene05315 | 0.849141 | 3.91E-60 | -1.69E-46 |
| MS.gene050551 | MS.gene05315 | 0.840817 | 6.92E-58 | -1.69E-46 |
| MS.gene051102 | MS.gene05315 | 0.8339 | 4.09E-56 | -1.69E-46 |
| MS.gene051381 | MS.gene05315 | 0.81427 | 1.68E-51 | -1.69E-46 |
| MS.gene052514 | MS.gene05315 | 0.827112 | 1.87E-54 | -1.69E-46 |
| MS.gene052609 | MS.gene05315 | 0.84379 | 1.13E-58 | -1.69E-46 |
| MS.gene053079 | MS.gene05315 | 0.808852 | 2.53E-50 | -1.69E-46 |
| MS.gene05315 | MS.gene055469 | 0.814131 | 1.80E-51 | -1.69E-46 |
| MS.gene05315 | MS.gene056507 | 0.809375 | 1.95E-50 | -1.69E-46 |
| MS.gene05315 | MS.gene058010 | 0.823041 | 1.71E-53 | -1.69E-46 |
| MS.gene05315 | MS.gene058292 | 0.834443 | 2.99E-56 | -1.69E-46 |
| MS.gene05315 | MS.gene060085 | 0.802969 | 4.36E-49 | -1.69E-46 |
| MS.gene05315 | MS.gene060933 | 0.807701 | 4.44E-50 | -1.69E-46 |
| MS.gene05315 | MS.gene061006 | 0.809845 | 1.55E-50 | -1.69E-46 |
| MS.gene05315 | MS.gene06133 | 0.830883 | 2.29E-55 | -1.69E-46 |
| MS.gene05315 | MS.gene061707 | 0.81038 | 1.19E-50 | -1.69E-46 |
| MS.gene05315 | MS.gene062042 | 0.807784 | 4.27E-50 | -1.69E-46 |
| MS.gene05315 | MS.gene06238 | 0.812399 | 4.32E-51 | -1.69E-46 |
| MS.gene05315 | MS.gene065979 | 0.809802 | 1.58E-50 | -1.69E-46 |
| MS.gene05315 | MS.gene06740 | 0.826542 | 2.56E-54 | -1.69E-46 |
| MS.gene05315 | MS.gene067966 | 0.803229 | 3.85E-49 | -1.69E-46 |
| MS.gene05315 | MS.gene06946 | 0.808929 | 2.43E-50 | -1.69E-46 |
| MS.gene05315 | MS.gene06958 | 0.826934 | 2.07E-54 | -1.69E-46 |
| MS.gene05315 | MS.gene06992 | 0.805332 | 1.40E-49 | -1.69E-46 |
| MS.gene05315 | MS.gene06993 | 0.806447 | 8.19E-50 | -1.69E-46 |
| MS.gene05315 | MS.gene072740 | 0.825136 | 5.53E-54 | -1.69E-46 |
| MS.gene05315 | MS.gene072762 | 0.801671 | 8.07E-49 | -1.69E-46 |
| MS.gene05315 | MS.gene073390 | 0.819957 | 8.84E-53 | -1.69E-46 |
| MS.gene05315 | MS.gene074604 | 0.813305 | 2.74E-51 | -1.69E-46 |
| MS.gene05315 | MS.gene07510 | 0.81271 | 3.70E-51 | -1.69E-46 |
| MS.gene05315 | MS.gene21632 | 0.810444 | 1.15E-50 | -1.69E-46 |
| MS.gene05315 | MS.gene21651 | 0.843574 | 1.29E-58 | -1.69E-46 |
| MS.gene05315 | MS.gene22321 | 0.811584 | 6.51E-51 | -1.69E-46 |
| MS.gene05315 | MS.gene24903 | 0.82318 | 1.59E-53 | -1.69E-46 |
| MS.gene05315 | MS.gene25966 | 0.803733 | 3.03E-49 | -1.69E-46 |
| MS.gene05315 | MS.gene26396 | 0.838073 | 3.57E-57 | -1.69E-46 |
| MS.gene05315 | MS.gene27287 | 0.80608 | 9.79E-50 | -1.69E-46 |
| MS.gene05315 | MS.gene27700 | 0.800494 | 1.40E-48 | -1.69E-46 |
| MS.gene05315 | MS.gene30198 | 0.824536 | 7.66E-54 | -1.69E-46 |
| MS.gene05315 | MS.gene30419 | 0.80491 | 1.72E-49 | -1.69E-46 |
| MS.gene05315 | MS.gene31667 | 0.807008 | 6.23E-50 | -1.69E-46 |
| MS.gene05315 | MS.gene32028 | 0.821588 | 3.73E-53 | -1.69E-46 |
| MS.gene05315 | MS.gene32134 | 0.818594 | 1.81E-52 | -1.69E-46 |
| MS.gene05315 | MS.gene32685 | 0.802642 | 5.09E-49 | -1.69E-46 |
| MS.gene05315 | MS.gene32742 | 0.809495 | 1.84E-50 | -1.69E-46 |
| MS.gene05315 | MS.gene32884 | 0.800272 | 1.56E-48 | -1.69E-46 |
| MS.gene05315 | MS.gene33135 | 0.824957 | 6.09E-54 | -1.69E-46 |
| MS.gene05315 | MS.gene33363 | 0.801617 | 8.28E-49 | -1.69E-46 |
| MS.gene05315 | MS.gene34515 | 0.815386 | 9.49E-52 | -1.69E-46 |
| MS.gene05315 | MS.gene35323 | 0.802589 | 5.22E-49 | -1.69E-46 |
| MS.gene05315 | MS.gene37007 | 0.804618 | 1.98E-49 | -1.69E-46 |
| MS.gene05315 | MS.gene39046 | 0.813964 | 1.96E-51 | -1.69E-46 |
| MS.gene05315 | MS.gene39253 | 0.811515 | 6.74E-51 | -1.69E-46 |
| MS.gene05315 | MS.gene39314 | 0.802146 | 6.44E-49 | -1.69E-46 |
| MS.gene05315 | MS.gene39529 | 0.841706 | 4.04E-58 | -1.69E-46 |
| MS.gene05315 | MS.gene40806 | 0.805476 | 1.31E-49 | -1.69E-46 |
| MS.gene05315 | MS.gene45613 | 0.815243 | 1.02E-51 | -1.69E-46 |
| MS.gene05315 | MS.gene47554 | 0.813561 | 2.40E-51 | -1.69E-46 |
| MS.gene05315 | MS.gene47581 | 0.850685 | 1.45E-60 | -1.69E-46 |
| MS.gene05315 | MS.gene47601 | 0.803525 | 3.34E-49 | -1.69E-46 |
| MS.gene05315 | MS.gene48283 | 0.823744 | 1.17E-53 | -1.69E-46 |
| MS.gene05315 | MS.gene49422 | 0.823761 | 1.16E-53 | -1.69E-46 |
| MS.gene05315 | MS.gene49423 | 0.805333 | 1.40E-49 | -1.69E-46 |
| MS.gene05315 | MS.gene49883 | 0.815901 | 7.29E-52 | -1.69E-46 |
| MS.gene05315 | MS.gene51029 | 0.808303 | 3.31E-50 | -1.69E-46 |
| MS.gene05315 | MS.gene51789 | 0.819521 | 1.11E-52 | -1.69E-46 |
| MS.gene05315 | MS.gene51929 | 0.865851 | 4.37E-65 | -1.69E-46 |
| MS.gene05315 | MS.gene53064 | 0.80443 | 2.17E-49 | -1.69E-46 |
| MS.gene05315 | MS.gene54158 | 0.816013 | 6.88E-52 | -1.69E-46 |
| MS.gene05315 | MS.gene56163 | 0.813843 | 2.08E-51 | -1.69E-46 |
| MS.gene05315 | MS.gene56717 | 0.802269 | 6.08E-49 | -1.69E-46 |
| MS.gene05315 | MS.gene58134 | 0.831609 | 1.51E-55 | -1.69E-46 |
| MS.gene05315 | MS.gene58617 | 0.804675 | 1.93E-49 | -1.69E-46 |
| MS.gene05315 | MS.gene59499 | 0.809406 | 1.92E-50 | -1.69E-46 |
| MS.gene05315 | MS.gene59979 | 0.815261 | 1.01E-51 | -1.69E-46 |
| MS.gene05315 | MS.gene60163 | 0.809035 | 2.31E-50 | -1.69E-46 |
| MS.gene05315 | MS.gene60935 | 0.803855 | 2.86E-49 | -1.69E-46 |
| MS.gene05315 | MS.gene62924 | 0.800475 | 1.42E-48 | -1.69E-46 |
| MS.gene05315 | MS.gene63930 | 0.816516 | 5.31E-52 | -1.69E-46 |
| MS.gene05315 | MS.gene64800 | 0.803838 | 2.88E-49 | -1.69E-46 |
| MS.gene05315 | MS.gene65300 | 0.81853 | 1.87E-52 | -1.69E-46 |
| MS.gene05315 | MS.gene66975 | 0.803099 | 4.10E-49 | -1.69E-46 |
| MS.gene05315 | MS.gene67553 | 0.813287 | 2.76E-51 | -1.69E-46 |
| MS.gene05315 | MS.gene68107 | 0.811084 | 8.36E-51 | -1.69E-46 |
| MS.gene05315 | MS.gene68360 | 0.800439 | 1.44E-48 | -1.69E-46 |
| MS.gene05315 | MS.gene68418 | 0.804471 | 2.13E-49 | -1.69E-46 |
| MS.gene05315 | MS.gene69389 | 0.830248 | 3.27E-55 | -1.69E-46 |
| MS.gene05315 | MS.gene71616 | 0.82023 | 7.65E-53 | -1.69E-46 |
| MS.gene05315 | MS.gene74902 | 0.819115 | 1.38E-52 | -1.69E-46 |
| MS.gene05315 | MS.gene74904 | 0.800424 | 1.45E-48 | -1.69E-46 |
| MS.gene05315 | MS.gene74932 | 0.821622 | 3.66E-53 | -1.69E-46 |
| MS.gene05315 | MS.gene75866 | 0.813429 | 2.57E-51 | -1.69E-46 |
| MS.gene05315 | MS.gene76317 | 0.819105 | 1.38E-52 | -1.69E-46 |
| MS.gene05315 | MS.gene76506 | 0.802192 | 6.30E-49 | -1.69E-46 |
| MS.gene05315 | MS.gene77356 | 0.80356 | 3.29E-49 | -1.69E-46 |
| MS.gene05315 | MS.gene79543 | 0.808506 | 3.00E-50 | -1.69E-46 |
| MS.gene05315 | MS.gene79797 | 0.800444 | 1.44E-48 | -1.69E-46 |
| MS.gene05315 | MS.gene80202 | 0.800792 | 1.22E-48 | -1.69E-46 |
| MS.gene05315 | MS.gene80574 | 0.816617 | 5.04E-52 | -1.69E-46 |
| MS.gene05315 | MS.gene82806 | 0.830386 | 3.02E-55 | -1.69E-46 |
| MS.gene05315 | MS.gene82989 | 0.832343 | 9.98E-56 | -1.69E-46 |
| MS.gene05315 | MS.gene83055 | 0.819924 | 8.99E-53 | -1.69E-46 |
| MS.gene05315 | MS.gene84454 | 0.833869 | 4.16E-56 | -1.69E-46 |
| MS.gene05315 | MS.gene84840 | 0.808372 | 3.20E-50 | -1.69E-46 |
| MS.gene05315 | MS.gene85122 | 0.802333 | 5.90E-49 | -1.69E-46 |
| MS.gene05315 | MS.gene86267 | 0.824118 | 9.60E-54 | -1.69E-46 |
| MS.gene05315 | MS.gene86844 | 0.812881 | 3.39E-51 | -1.69E-46 |
| MS.gene05315 | MS.gene87622 | 0.809129 | 2.20E-50 | -1.69E-46 |
| MS.gene05315 | MS.gene88032 | 0.800464 | 1.42E-48 | -1.69E-46 |
| MS.gene05315 | MS.gene90386 | 0.832143 | 1.12E-55 | -1.69E-46 |
| MS.gene05315 | MS.gene90525 | 0.833653 | 4.71E-56 | -1.69E-46 |
| MS.gene05315 | MS.gene90642 | 0.805828 | 1.11E-49 | -1.69E-46 |
| MS.gene05315 | MS.gene91307 | 0.817505 | 3.19E-52 | -1.69E-46 |
| MS.gene05315 | MS.gene93728 | 0.813307 | 2.74E-51 | -1.69E-46 |
| MS.gene05315 | MS.gene94920 | 0.802806 | 4.71E-49 | -1.69E-46 |
| MS.gene05315 | MS.gene95096 | 0.800906 | 1.16E-48 | -1.69E-46 |
| MS.gene05315 | MS.gene96191 | 0.805745 | 1.15E-49 | -1.69E-46 |
| MS.gene05315 | MS.gene97176 | 0.802303 | 5.98E-49 | -1.69E-46 |
| MS.gene05315 | MS.gene98394 | 0.809282 | 2.04E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05315.t1 | MTR_6g080420 | 92.364 | 275 | 11 | 1 | 207 | 471 | 1 | 275 | 2.60e-177 | 507 |
| MS.gene05315.t1 | MTR_8g106700 | 81.132 | 265 | 39 | 4 | 209 | 471 | 2 | 257 | 9.82e-145 | 424 |
| MS.gene05315.t1 | MTR_8g106700 | 77.358 | 265 | 28 | 3 | 209 | 471 | 2 | 236 | 2.29e-136 | 402 |
| MS.gene05315.t1 | MTR_8g106700 | 85.022 | 227 | 29 | 2 | 247 | 471 | 7 | 230 | 8.20e-133 | 392 |
| MS.gene05315.t1 | MTR_1g024175 | 46.111 | 180 | 97 | 0 | 288 | 467 | 8 | 187 | 9.89e-53 | 184 |
| MS.gene05315.t1 | MTR_6g021800 | 46.667 | 180 | 96 | 0 | 285 | 464 | 5 | 184 | 8.66e-52 | 182 |
| MS.gene05315.t1 | MTR_6g021805 | 46.667 | 180 | 96 | 0 | 285 | 464 | 5 | 184 | 8.66e-52 | 182 |
| MS.gene05315.t1 | MTR_1g095703 | 46.667 | 180 | 96 | 0 | 285 | 464 | 5 | 184 | 1.04e-51 | 181 |
| MS.gene05315.t1 | MTR_1g495705 | 46.667 | 180 | 96 | 0 | 285 | 464 | 5 | 184 | 1.16e-51 | 181 |
| MS.gene05315.t1 | MTR_1g095650 | 46.667 | 180 | 96 | 0 | 285 | 464 | 5 | 184 | 1.16e-51 | 181 |
| MS.gene05315.t1 | MTR_4g014810 | 46.111 | 180 | 97 | 0 | 285 | 464 | 5 | 184 | 1.17e-51 | 181 |
| MS.gene05315.t1 | MTR_8g008740 | 45.856 | 181 | 97 | 1 | 288 | 467 | 8 | 188 | 2.80e-49 | 176 |
| MS.gene05315.t1 | MTR_1g085410 | 44.324 | 185 | 103 | 0 | 281 | 465 | 1 | 185 | 4.59e-49 | 174 |
| MS.gene05315.t1 | MTR_8g087950 | 46.893 | 177 | 91 | 2 | 288 | 463 | 276 | 450 | 8.13e-48 | 176 |
| MS.gene05315.t1 | MTR_8g087950 | 46.893 | 177 | 91 | 2 | 288 | 463 | 349 | 523 | 1.39e-47 | 176 |
| MS.gene05315.t1 | MTR_2g080210 | 44.022 | 184 | 100 | 2 | 285 | 467 | 7 | 188 | 5.69e-47 | 168 |
| MS.gene05315.t1 | MTR_1g013680 | 43.716 | 183 | 100 | 1 | 285 | 464 | 5 | 187 | 4.35e-46 | 167 |
| MS.gene05315.t1 | MTR_1g101870 | 42.857 | 182 | 102 | 1 | 284 | 465 | 4 | 183 | 4.08e-43 | 163 |
| MS.gene05315.t1 | MTR_1g101870 | 42.857 | 182 | 102 | 1 | 284 | 465 | 4 | 183 | 4.41e-43 | 163 |
| MS.gene05315.t1 | MTR_1g024165 | 47.101 | 138 | 73 | 0 | 288 | 425 | 12 | 149 | 1.54e-39 | 140 |
| MS.gene05315.t1 | MTR_8g014590 | 40.541 | 185 | 108 | 1 | 285 | 467 | 5 | 189 | 4.18e-39 | 147 |
| MS.gene05315.t1 | MTR_2g082730 | 39.572 | 187 | 98 | 1 | 281 | 467 | 1 | 172 | 2.79e-38 | 146 |
| MS.gene05315.t1 | MTR_1g101880 | 42.177 | 147 | 85 | 0 | 288 | 434 | 8 | 154 | 5.53e-36 | 134 |
| MS.gene05315.t1 | MTR_1g089520 | 38.674 | 181 | 94 | 2 | 285 | 465 | 6 | 169 | 1.56e-35 | 139 |
| MS.gene05315.t1 | MTR_8g013910 | 36.464 | 181 | 112 | 2 | 284 | 463 | 4 | 182 | 5.28e-32 | 127 |
| MS.gene05315.t1 | MTR_5g037350 | 31.944 | 216 | 110 | 1 | 285 | 463 | 15 | 230 | 1.01e-30 | 125 |
| MS.gene05315.t1 | MTR_2g037420 | 35.870 | 184 | 101 | 1 | 281 | 464 | 1 | 167 | 5.52e-29 | 119 |
| MS.gene05315.t1 | MTR_2g082665 | 33.690 | 187 | 83 | 1 | 281 | 467 | 1 | 146 | 2.65e-25 | 108 |
| MS.gene05315.t1 | MTR_3g076660 | 34.940 | 166 | 86 | 2 | 271 | 436 | 76 | 219 | 4.56e-22 | 99.4 |
| MS.gene05315.t1 | MTR_2g021300 | 37.908 | 153 | 71 | 4 | 285 | 436 | 48 | 177 | 5.04e-20 | 92.8 |
| MS.gene05315.t1 | MTR_1g095650 | 51.163 | 86 | 42 | 0 | 382 | 467 | 1 | 86 | 2.25e-19 | 89.7 |
| MS.gene05315.t1 | MTR_8g014950 | 37.624 | 101 | 63 | 0 | 284 | 384 | 5 | 105 | 4.95e-19 | 82.8 |
| MS.gene05315.t1 | MTR_7g094460 | 35.526 | 152 | 76 | 3 | 285 | 436 | 61 | 190 | 2.53e-18 | 87.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05315.t1 | AT1G18070 | 68.750 | 288 | 61 | 4 | 209 | 471 | 2 | 285 | 5.72e-125 | 374 |
| MS.gene05315.t1 | AT1G18070 | 68.750 | 288 | 61 | 4 | 209 | 471 | 2 | 285 | 5.72e-125 | 374 |
| MS.gene05315.t1 | AT1G18070 | 68.750 | 288 | 61 | 4 | 209 | 471 | 2 | 285 | 6.17e-125 | 375 |
| MS.gene05315.t1 | AT1G07930 | 46.667 | 180 | 96 | 0 | 285 | 464 | 5 | 184 | 1.93e-52 | 181 |
| MS.gene05315.t1 | AT5G60390 | 46.667 | 180 | 96 | 0 | 285 | 464 | 5 | 184 | 1.24e-51 | 181 |
| MS.gene05315.t1 | AT5G60390 | 46.667 | 180 | 96 | 0 | 285 | 464 | 5 | 184 | 1.24e-51 | 181 |
| MS.gene05315.t1 | AT5G60390 | 46.667 | 180 | 96 | 0 | 285 | 464 | 5 | 184 | 1.24e-51 | 181 |
| MS.gene05315.t1 | AT1G07920 | 46.667 | 180 | 96 | 0 | 285 | 464 | 5 | 184 | 1.24e-51 | 181 |
| MS.gene05315.t1 | AT1G07940 | 46.667 | 180 | 96 | 0 | 285 | 464 | 5 | 184 | 1.24e-51 | 181 |
| MS.gene05315.t1 | AT1G07940 | 46.667 | 180 | 96 | 0 | 285 | 464 | 5 | 184 | 1.24e-51 | 181 |
| MS.gene05315.t1 | AT1G07940 | 46.667 | 180 | 96 | 0 | 285 | 464 | 5 | 184 | 1.24e-51 | 181 |
| MS.gene05315.t1 | AT1G07940 | 46.667 | 180 | 96 | 0 | 285 | 464 | 5 | 184 | 1.24e-51 | 181 |
| MS.gene05315.t1 | AT1G07930 | 46.667 | 180 | 96 | 0 | 285 | 464 | 5 | 184 | 1.24e-51 | 181 |
| MS.gene05315.t1 | AT5G10630 | 41.799 | 189 | 104 | 3 | 276 | 463 | 231 | 414 | 2.09e-43 | 163 |
| MS.gene05315.t1 | AT5G10630 | 41.799 | 189 | 104 | 3 | 276 | 463 | 232 | 415 | 2.11e-43 | 163 |
| MS.gene05315.t1 | AT5G10630 | 41.799 | 189 | 104 | 3 | 276 | 463 | 232 | 415 | 2.11e-43 | 163 |
| MS.gene05315.t1 | AT5G10630 | 41.799 | 189 | 104 | 3 | 276 | 463 | 302 | 485 | 2.75e-43 | 163 |
| MS.gene05315.t1 | AT5G10630 | 41.799 | 189 | 104 | 3 | 276 | 463 | 307 | 490 | 3.13e-43 | 163 |
| MS.gene05315.t1 | AT4G20360 | 35.294 | 153 | 77 | 2 | 284 | 436 | 76 | 206 | 4.05e-21 | 96.3 |
| MS.gene05315.t1 | AT4G20360 | 35.294 | 153 | 77 | 2 | 284 | 436 | 76 | 206 | 4.05e-21 | 96.3 |
| MS.gene05315.t1 | AT4G02930 | 34.177 | 158 | 82 | 3 | 279 | 436 | 59 | 194 | 8.51e-18 | 86.3 |
Find 115 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GACATCAAGGTTTACGATTT+TGG | 0.090601 | 6.3:+74646894 | MS.gene05315:CDS |
| CACTTAAATGTTGTATTTAT+TGG | 0.147319 | 6.3:+74643540 | MS.gene05315:CDS |
| CAATAAGCCCCAACCAATTA+TGG | 0.178687 | 6.3:+74631203 | MS.gene05315:CDS |
| CTTCGAGTCCCAACCAATTA+TGG | 0.212009 | 6.3:+74631236 | MS.gene05315:CDS |
| GCTTGTTGTTGTTAATAAAA+TGG | 0.241079 | 6.3:+74648387 | MS.gene05315:CDS |
| CCACAGGTCCCAACCAATTA+TGG | 0.248585 | 6.3:+74631128 | MS.gene05315:CDS |
| ACTTCTGTCTTTCCCTCATC+TGG | 0.250286 | 6.3:-74642130 | None:intergenic |
| GTACGGAATTGGAAATTTAC+AGG | 0.272163 | 6.3:+74618986 | MS.gene05315:CDS |
| AAATTTAAATGATAGGTATA+TGG | 0.273003 | 6.3:+74646670 | MS.gene05315:intron |
| CACGGTGGAGTGTTCATAAT+TGG | 0.280010 | 6.3:-74631076 | None:intergenic |
| ACTACGATGTTGAGTTGTTT+TGG | 0.296028 | 6.3:+74614182 | MS.gene05315:CDS |
| TCAGCCCGGGTGATGTGGTT+AGG | 0.309500 | 6.3:+74618951 | MS.gene05315:intron |
| AAGTCTTAAGTGCCCATAAT+TGG | 0.316465 | 6.3:-74631216 | None:intergenic |
| TGTCGTGTGGTGCCCATAAT+TGG | 0.320380 | 6.3:-74631249 | None:intergenic |
| TCTATTAAGACTGAGATAAA+AGG | 0.341814 | 6.3:+74643308 | MS.gene05315:CDS |
| CAACATCGTAGTACGATACT+AGG | 0.349475 | 6.3:-74614171 | None:intergenic |
| AATTCTTTATTTCTTTGTGC+TGG | 0.373442 | 6.3:-74614126 | None:intergenic |
| AGAAATTCGGTTGGGGAAGA+AGG | 0.379712 | 6.3:-74640515 | None:intergenic |
| GCTTACCTTCAACAGATTGT+TGG | 0.380365 | 6.3:+74634304 | MS.gene05315:CDS |
| GGGTATGTGGTGTCCATAAT+TGG | 0.381192 | 6.3:-74631106 | None:intergenic |
| TTAAGTGCCCATAATTGGTT+GGG | 0.382354 | 6.3:-74631211 | None:intergenic |
| ACCGTGGCTCCAACCAATTA+TGG | 0.384883 | 6.3:+74631093 | MS.gene05315:CDS |
| TTTGGTGTCCATAATTGGTT+GGG | 0.389034 | 6.3:-74631136 | None:intergenic |
| GGTGCATCTCAAGCTGATAT+TGG | 0.389282 | 6.3:+74648150 | MS.gene05315:CDS |
| AAGCTTTCTCAACATGTCTA+AGG | 0.394302 | 6.3:-74634285 | None:intergenic |
| CGAAAGGGAGAATTTGAAAC+TGG | 0.395507 | 6.3:+74648298 | MS.gene05315:CDS |
| GATACCATTTCTTAAGCAAT+CGG | 0.399399 | 6.3:+74648552 | MS.gene05315:CDS |
| GGCCACTGAGGTAAAGTATC+TGG | 0.400510 | 6.3:-74645393 | None:intergenic |
| ATGTTAACTTAGTACGGAAT+TGG | 0.410996 | 6.3:+74618975 | MS.gene05315:CDS |
| CCATAATTGGTTGGGACCTG+TGG | 0.416901 | 6.3:-74631128 | None:intergenic |
| TAGGTATATGGCTTACATAA+TGG | 0.418640 | 6.3:+74646682 | MS.gene05315:intron |
| TCAAGCTGATATTGGAGTAT+TGG | 0.421955 | 6.3:+74648158 | MS.gene05315:CDS |
| ATTGAAGAGAGTGAGAAATT+CGG | 0.426193 | 6.3:-74640528 | None:intergenic |
| CAGCCCGGGTGATGTGGTTA+GGG | 0.431844 | 6.3:+74618952 | MS.gene05315:intron |
| GATCTTGATGCCGAGAGATC+TGG | 0.433501 | 6.3:+74627756 | MS.gene05315:intron |
| AGAGAGTGAGAAATTCGGTT+GGG | 0.434710 | 6.3:-74640523 | None:intergenic |
| TTCGAGTCCCAACCAATTAT+GGG | 0.437171 | 6.3:+74631237 | MS.gene05315:CDS |
| AATAAGCCCCAACCAATTAT+GGG | 0.438036 | 6.3:+74631204 | MS.gene05315:CDS |
| TGTGCTTGAACCTCTTGCTT+AGG | 0.446201 | 6.3:-74643284 | None:intergenic |
| TAACCCGATTGCTTAAGAAA+TGG | 0.453560 | 6.3:-74648556 | None:intergenic |
| GTTGGGACCTGTGGGGTATG+TGG | 0.454567 | 6.3:-74631119 | None:intergenic |
| AGTTCTTCGTAGATATTTGT+GGG | 0.457699 | 6.3:+74631047 | MS.gene05315:CDS |
| AAGTTCTTCGTAGATATTTG+TGG | 0.458407 | 6.3:+74631046 | MS.gene05315:CDS |
| ACCAATTATGAGCACTCAAA+AGG | 0.462606 | 6.3:+74631177 | MS.gene05315:CDS |
| GATGCACCACTGATCATGTT+AGG | 0.462975 | 6.3:-74648135 | None:intergenic |
| CATAATTGGTTGGGACCTGT+GGG | 0.475433 | 6.3:-74631127 | None:intergenic |
| CGCTTCACTTCATCCATGGC+AGG | 0.478098 | 6.3:+74640563 | MS.gene05315:CDS |
| TCTTTACAGCTTGAGTCAGC+AGG | 0.484486 | 6.3:+74641942 | MS.gene05315:CDS |
| CAGGGAAAGACAGTTGAAGT+TGG | 0.485880 | 6.3:+74646850 | MS.gene05315:intron |
| GAAATTCGGTTGGGGAAGAA+GGG | 0.495978 | 6.3:-74640514 | None:intergenic |
| TCCATAATTGGTTGGAGCCA+CGG | 0.497635 | 6.3:-74631094 | None:intergenic |
| GTTGTATTTATTGGTCATGT+GGG | 0.498005 | 6.3:+74643549 | MS.gene05315:CDS |
| GATACTTTACCTCAGTGGCC+AGG | 0.503870 | 6.3:+74645396 | MS.gene05315:CDS |
| ATACCATTTCTTAAGCAATC+GGG | 0.506394 | 6.3:+74648553 | MS.gene05315:CDS |
| TTTGAAACTGGATATGAGAA+AGG | 0.507954 | 6.3:+74648310 | MS.gene05315:CDS |
| GGATTGTCCGTTCATCAACC+TGG | 0.510942 | 6.3:-74645414 | None:intergenic |
| CACCATCTCACCAGATCTCT+CGG | 0.511498 | 6.3:-74627766 | None:intergenic |
| CTTAAGTGCCCATAATTGGT+TGG | 0.515295 | 6.3:-74631212 | None:intergenic |
| TTGCAAGCTGAACATGTTCA+CGG | 0.515802 | 6.3:-74648342 | None:intergenic |
| GAGAGTGAGAAATTCGGTTG+GGG | 0.516787 | 6.3:-74640522 | None:intergenic |
| TTAGGGATGTTAACTTAGTA+CGG | 0.523514 | 6.3:+74618969 | MS.gene05315:CDS |
| TCAGGTAATTTCTGCTCGAA+AGG | 0.523981 | 6.3:+74648282 | MS.gene05315:intron |
| TGTGGTGCCCATAATTGGTT+GGG | 0.527622 | 6.3:-74631244 | None:intergenic |
| AATGCATAAGGGAATGATAG+AGG | 0.531988 | 6.3:+74614093 | None:intergenic |
| TGCAAGCTGAACATGTTCAC+GGG | 0.533344 | 6.3:-74648341 | None:intergenic |
| TGAGTGCTCATAATTGGTTG+AGG | 0.537219 | 6.3:-74631172 | None:intergenic |
| ACATCCCTAACCACATCACC+CGG | 0.537995 | 6.3:-74618956 | None:intergenic |
| TCCTTTCAGCCCGGGTGATG+TGG | 0.542191 | 6.3:+74618946 | MS.gene05315:intron |
| TCATCTTCTTCAACTTGCAC+CGG | 0.542703 | 6.3:-74643492 | None:intergenic |
| TAAGTGCCCATAATTGGTTG+GGG | 0.542912 | 6.3:-74631210 | None:intergenic |
| CAGGTAATTTCTGCTCGAAA+GGG | 0.543026 | 6.3:+74648283 | MS.gene05315:intron |
| TGAGGGAAAGACAGAAGTTG+AGG | 0.544648 | 6.3:+74642135 | MS.gene05315:CDS |
| TCGGGTTACAATGTGAAGAA+AGG | 0.545454 | 6.3:+74648571 | MS.gene05315:CDS |
| AAGAGAGTGAGAAATTCGGT+TGG | 0.546520 | 6.3:-74640524 | None:intergenic |
| TCTTCTTCAACTTGCACCGG+AGG | 0.554751 | 6.3:-74643489 | None:intergenic |
| CATGTACACAATACGAGTAT+CGG | 0.555684 | 6.3:+74648647 | MS.gene05315:CDS |
| GTGTGGTGCCCATAATTGGT+TGG | 0.561056 | 6.3:-74631245 | None:intergenic |
| GATTCGCTTCACTTCATCCA+TGG | 0.562089 | 6.3:+74640559 | MS.gene05315:CDS |
| GACAGATAAGGAAATTCCTC+CGG | 0.562432 | 6.3:+74643473 | MS.gene05315:intron |
| TGTTGTATTTATTGGTCATG+TGG | 0.563048 | 6.3:+74643548 | MS.gene05315:CDS |
| GGACACAAATGAGGAAGAGA+GGG | 0.567260 | 6.3:+74646703 | MS.gene05315:CDS |
| AGATGATGAATTACCTGCCA+TGG | 0.568680 | 6.3:-74640576 | None:intergenic |
| GATGCTGGTAAGTCTACTAC+AGG | 0.572715 | 6.3:+74645367 | MS.gene05315:intron |
| GGATGATCCTACAGTGCAGT+GGG | 0.584143 | 6.3:+74648408 | MS.gene05315:CDS |
| AGGACAAAAGTAGAGAAAGC+TGG | 0.588639 | 6.3:+74645458 | MS.gene05315:CDS |
| TTACATAATGGACACAAATG+AGG | 0.595469 | 6.3:+74646694 | MS.gene05315:CDS |
| ATGTGGTGTCCATAATTGGT+TGG | 0.599422 | 6.3:-74631102 | None:intergenic |
| AACATCGTAGTACGATACTA+GGG | 0.600748 | 6.3:-74614170 | None:intergenic |
| GACATAGCAGAAGACAGCAA+TGG | 0.602824 | 6.3:+74642097 | MS.gene05315:intron |
| GGAGTTGTAAATCCAGATGA+GGG | 0.612506 | 6.3:+74642118 | MS.gene05315:CDS |
| CAAGCAGCTTAGACACACCC+AGG | 0.613480 | 6.3:-74648369 | None:intergenic |
| GTGGCCAATGTTAGATATCG+AGG | 0.613613 | 6.3:+74641908 | MS.gene05315:intron |
| TGGACACAAATGAGGAAGAG+AGG | 0.615049 | 6.3:+74646702 | MS.gene05315:CDS |
| TGGAGTTGTAAATCCAGATG+AGG | 0.616106 | 6.3:+74642117 | MS.gene05315:CDS |
| GGTTTCAGATCCTAAGCAAG+AGG | 0.618742 | 6.3:+74643274 | MS.gene05315:intron |
| CTGACTCAAGCTGTAAAGAA+CGG | 0.638319 | 6.3:-74641938 | None:intergenic |
| AAAATACGAGAAAGAAGCCA+AGG | 0.641054 | 6.3:+74645438 | MS.gene05315:CDS |
| TGGATGATCCTACAGTGCAG+TGG | 0.648027 | 6.3:+74648407 | MS.gene05315:CDS |
| TCAGTGGCCAGGTTGATGAA+CGG | 0.648307 | 6.3:+74645407 | MS.gene05315:CDS |
| TATGTTCCTAACATGATCAG+TGG | 0.655963 | 6.3:+74648129 | MS.gene05315:CDS |
| GAAACTGGATATGAGAAAGG+TGG | 0.661890 | 6.3:+74648313 | MS.gene05315:CDS |
| ATGGACACCACATACCCCAC+AGG | 0.662846 | 6.3:+74631112 | MS.gene05315:CDS |
| GGCCAGATACTTTACCTCAG+TGG | 0.663074 | 6.3:+74645391 | MS.gene05315:CDS |
| TGCATACATTCATTGTCGTG+TGG | 0.670174 | 6.3:-74631262 | None:intergenic |
| GAATCTGCTAATACACAACA+AGG | 0.673726 | 6.3:+74642157 | MS.gene05315:CDS |
| CATCCCTAACCACATCACCC+GGG | 0.674860 | 6.3:-74618955 | None:intergenic |
| GCTGGTAAGTCTACTACAGG+AGG | 0.675855 | 6.3:+74645370 | MS.gene05315:CDS |
| ATAATTGGTTGGGACCTGTG+GGG | 0.691625 | 6.3:-74631126 | None:intergenic |
| AAGCAGCTTAGACACACCCA+GGG | 0.698669 | 6.3:-74648368 | None:intergenic |
| AAATGAGGAAGAGAGGGCAA+AGG | 0.704381 | 6.3:+74646709 | MS.gene05315:CDS |
| GTTCATCAACCTGGCCACTG+AGG | 0.716402 | 6.3:-74645405 | None:intergenic |
| GCCAATGTTAGATATCGAGG+AGG | 0.722181 | 6.3:+74641911 | MS.gene05315:intron |
| GCAAGTTGAAGAAGATGACG+CGG | 0.729784 | 6.3:+74643497 | MS.gene05315:CDS |
| ATAATTGGTTGGAGCCACGG+TGG | 0.739124 | 6.3:-74631091 | None:intergenic |
| CAATTATGAACACTCCACCG+TGG | 0.778220 | 6.3:+74631077 | MS.gene05315:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.3 | gene | 74614106 | 74648675 | 74614106 | ID=MS.gene05315 |
| chr6.3 | mRNA | 74614106 | 74648675 | 74614106 | ID=MS.gene05315.t1;Parent=MS.gene05315 |
| chr6.3 | exon | 74614106 | 74614239 | 74614106 | ID=MS.gene05315.t1.exon1;Parent=MS.gene05315.t1 |
| chr6.3 | CDS | 74614106 | 74614239 | 74614106 | ID=cds.MS.gene05315.t1;Parent=MS.gene05315.t1 |
| chr6.3 | exon | 74618955 | 74619007 | 74618955 | ID=MS.gene05315.t1.exon2;Parent=MS.gene05315.t1 |
| chr6.3 | CDS | 74618955 | 74619007 | 74618955 | ID=cds.MS.gene05315.t1;Parent=MS.gene05315.t1 |
| chr6.3 | exon | 74627757 | 74627777 | 74627757 | ID=MS.gene05315.t1.exon3;Parent=MS.gene05315.t1 |
| chr6.3 | CDS | 74627757 | 74627777 | 74627757 | ID=cds.MS.gene05315.t1;Parent=MS.gene05315.t1 |
| chr6.3 | exon | 74631038 | 74631277 | 74631038 | ID=MS.gene05315.t1.exon4;Parent=MS.gene05315.t1 |
| chr6.3 | CDS | 74631038 | 74631277 | 74631038 | ID=cds.MS.gene05315.t1;Parent=MS.gene05315.t1 |
| chr6.3 | exon | 74634284 | 74634384 | 74634284 | ID=MS.gene05315.t1.exon5;Parent=MS.gene05315.t1 |
| chr6.3 | CDS | 74634284 | 74634384 | 74634284 | ID=cds.MS.gene05315.t1;Parent=MS.gene05315.t1 |
| chr6.3 | exon | 74640509 | 74640584 | 74640509 | ID=MS.gene05315.t1.exon6;Parent=MS.gene05315.t1 |
| chr6.3 | CDS | 74640509 | 74640584 | 74640509 | ID=cds.MS.gene05315.t1;Parent=MS.gene05315.t1 |
| chr6.3 | exon | 74641922 | 74641963 | 74641922 | ID=MS.gene05315.t1.exon7;Parent=MS.gene05315.t1 |
| chr6.3 | CDS | 74641922 | 74641963 | 74641922 | ID=cds.MS.gene05315.t1;Parent=MS.gene05315.t1 |
| chr6.3 | exon | 74642098 | 74642178 | 74642098 | ID=MS.gene05315.t1.exon8;Parent=MS.gene05315.t1 |
| chr6.3 | CDS | 74642098 | 74642178 | 74642098 | ID=cds.MS.gene05315.t1;Parent=MS.gene05315.t1 |
| chr6.3 | exon | 74643282 | 74643329 | 74643282 | ID=MS.gene05315.t1.exon9;Parent=MS.gene05315.t1 |
| chr6.3 | CDS | 74643282 | 74643329 | 74643282 | ID=cds.MS.gene05315.t1;Parent=MS.gene05315.t1 |
| chr6.3 | exon | 74643478 | 74643570 | 74643478 | ID=MS.gene05315.t1.exon10;Parent=MS.gene05315.t1 |
| chr6.3 | CDS | 74643478 | 74643570 | 74643478 | ID=cds.MS.gene05315.t1;Parent=MS.gene05315.t1 |
| chr6.3 | exon | 74645368 | 74645479 | 74645368 | ID=MS.gene05315.t1.exon11;Parent=MS.gene05315.t1 |
| chr6.3 | CDS | 74645368 | 74645479 | 74645368 | ID=cds.MS.gene05315.t1;Parent=MS.gene05315.t1 |
| chr6.3 | exon | 74646685 | 74646730 | 74646685 | ID=MS.gene05315.t1.exon12;Parent=MS.gene05315.t1 |
| chr6.3 | CDS | 74646685 | 74646730 | 74646685 | ID=cds.MS.gene05315.t1;Parent=MS.gene05315.t1 |
| chr6.3 | exon | 74646853 | 74646924 | 74646853 | ID=MS.gene05315.t1.exon13;Parent=MS.gene05315.t1 |
| chr6.3 | CDS | 74646853 | 74646924 | 74646853 | ID=cds.MS.gene05315.t1;Parent=MS.gene05315.t1 |
| chr6.3 | exon | 74648117 | 74648179 | 74648117 | ID=MS.gene05315.t1.exon14;Parent=MS.gene05315.t1 |
| chr6.3 | CDS | 74648117 | 74648179 | 74648117 | ID=cds.MS.gene05315.t1;Parent=MS.gene05315.t1 |
| chr6.3 | exon | 74648286 | 74648440 | 74648286 | ID=MS.gene05315.t1.exon15;Parent=MS.gene05315.t1 |
| chr6.3 | CDS | 74648286 | 74648440 | 74648286 | ID=cds.MS.gene05315.t1;Parent=MS.gene05315.t1 |
| chr6.3 | exon | 74648528 | 74648592 | 74648528 | ID=MS.gene05315.t1.exon16;Parent=MS.gene05315.t1 |
| chr6.3 | CDS | 74648528 | 74648592 | 74648528 | ID=cds.MS.gene05315.t1;Parent=MS.gene05315.t1 |
| chr6.3 | exon | 74648632 | 74648675 | 74648632 | ID=MS.gene05315.t1.exon17;Parent=MS.gene05315.t1 |
| chr6.3 | CDS | 74648632 | 74648675 | 74648632 | ID=cds.MS.gene05315.t1;Parent=MS.gene05315.t1 |
| Gene Sequence |
| Protein sequence |
