Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26861.t1 | 1ZGJ_A | 85.8 | 239 | 3 | 1 | 1 | 208 | 116 | 354 | 2.60E-108 | 401.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26861.t1 | Q29U70 | 85.8 | 239 | 3 | 1 | 1 | 208 | 126 | 364 | 6.3e-111 | 401.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26861.t1 | A0A072UNK8 | 85.8 | 239 | 3 | 1 | 1 | 208 | 126 | 364 | 1.9e-108 | 401.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050883 | MS.gene26861 | 0.843469 | 1.37E-58 | -1.69E-46 |
| MS.gene051127 | MS.gene26861 | 0.805609 | 1.23E-49 | -1.69E-46 |
| MS.gene051341 | MS.gene26861 | 0.834651 | 2.65E-56 | -1.69E-46 |
| MS.gene053826 | MS.gene26861 | 0.801517 | 8.68E-49 | -1.69E-46 |
| MS.gene055961 | MS.gene26861 | 0.810542 | 1.09E-50 | -1.69E-46 |
| MS.gene056310 | MS.gene26861 | 0.808295 | 3.32E-50 | -1.69E-46 |
| MS.gene056311 | MS.gene26861 | 0.816601 | 5.09E-52 | -1.69E-46 |
| MS.gene058109 | MS.gene26861 | 0.822219 | 2.66E-53 | -1.69E-46 |
| MS.gene058111 | MS.gene26861 | 0.834321 | 3.21E-56 | -1.69E-46 |
| MS.gene058171 | MS.gene26861 | 0.832285 | 1.03E-55 | -1.69E-46 |
| MS.gene058173 | MS.gene26861 | 0.832051 | 1.18E-55 | -1.69E-46 |
| MS.gene058175 | MS.gene26861 | 0.831036 | 2.10E-55 | -1.69E-46 |
| MS.gene058493 | MS.gene26861 | 0.803886 | 2.81E-49 | -1.69E-46 |
| MS.gene058494 | MS.gene26861 | 0.802932 | 4.44E-49 | -1.69E-46 |
| MS.gene058628 | MS.gene26861 | 0.808957 | 2.40E-50 | -1.69E-46 |
| MS.gene058629 | MS.gene26861 | 0.805736 | 1.16E-49 | -1.69E-46 |
| MS.gene058630 | MS.gene26861 | 0.811758 | 5.97E-51 | -1.69E-46 |
| MS.gene059045 | MS.gene26861 | 0.810566 | 1.08E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 35 sgRNAs with CRISPR-Local
Find 61 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCTGTTCTTCAATATCTTTA+AGG | 0.195840 | 4.1:-22213363 | None:intergenic |
| GTGCAACAGGGGAGAGCTTT+TGG | 0.222303 | 4.1:+22212685 | MS.gene26861:CDS |
| CGAACACGCGTTTGTTCTCT+TGG | 0.271846 | 4.1:-22212797 | None:intergenic |
| CAAACGCGTGTTCGAGGGTT+TGG | 0.283225 | 4.1:+22212806 | MS.gene26861:CDS |
| TACAGTGGGTTCTGCATGAT+TGG | 0.326455 | 4.1:+22213327 | MS.gene26861:intron |
| ACACTGTTTGAGTGTGCAAC+AGG | 0.344866 | 4.1:+22212672 | MS.gene26861:CDS |
| GTGATGCTTACAATGTTTCT+TGG | 0.364082 | 4.1:+22213500 | MS.gene26861:CDS |
| TGGATTAACTGAACTGCAAT+TGG | 0.377228 | 4.1:+22213466 | MS.gene26861:CDS |
| AAAACAGTGCATTTAAGGTT+AGG | 0.380135 | 4.1:-22212891 | None:intergenic |
| GTTGATGTTGGAGGTGGTAC+TGG | 0.401447 | 4.1:+22212837 | MS.gene26861:CDS |
| TTGGAGTCTCTTGTTGATGT+TGG | 0.404723 | 4.1:+22212825 | MS.gene26861:CDS |
| CTCCACATGTCTAAAGAACT+TGG | 0.410016 | 4.1:-22212612 | None:intergenic |
| AAGAAAGAACAAAGCAAGAA+TGG | 0.426132 | 4.1:+22213525 | MS.gene26861:CDS |
| ACTGCAATTGGACTATGATT+TGG | 0.427028 | 4.1:+22213478 | MS.gene26861:CDS |
| GAGAGACTTGAAGCCAGAAA+TGG | 0.427559 | 4.1:-22213594 | None:intergenic |
| AGAGACTTGAAGCCAGAAAT+GGG | 0.439445 | 4.1:-22213593 | None:intergenic |
| AGAGAACAAACGCGTGTTCG+AGG | 0.444395 | 4.1:+22212800 | MS.gene26861:CDS |
| TGTGGAGTTCCTCAGAGAAA+TGG | 0.491399 | 4.1:+22212628 | MS.gene26861:CDS |
| GAGTCTCTTGTTGATGTTGG+AGG | 0.496314 | 4.1:+22212828 | MS.gene26861:CDS |
| ATCCAAGTTCTTTAGACATG+TGG | 0.496638 | 4.1:+22212610 | MS.gene26861:CDS |
| GAGTATGTTTCAAGATGCTA+TGG | 0.498973 | 4.1:+22212743 | MS.gene26861:CDS |
| AGAAAGAACAAAGCAAGAAT+GGG | 0.507206 | 4.1:+22213526 | MS.gene26861:CDS |
| GAAATGGTTCAATGAAGATA+AGG | 0.516608 | 4.1:+22212644 | MS.gene26861:CDS |
| GAGAACAAACGCGTGTTCGA+GGG | 0.542946 | 4.1:+22212801 | MS.gene26861:CDS |
| ATTTCACACAAAGGAAAGGA+TGG | 0.547784 | 4.1:+22213395 | MS.gene26861:CDS |
| TCTCTTGTTGATGTTGGAGG+TGG | 0.554073 | 4.1:+22212831 | MS.gene26861:CDS |
| AGCAATTTCACACAAAGGAA+AGG | 0.577136 | 4.1:+22213391 | MS.gene26861:CDS |
| CACTGTTTGAGTGTGCAACA+GGG | 0.603315 | 4.1:+22212673 | MS.gene26861:CDS |
| GATGTTGGAGGTGGTACTGG+TGG | 0.610342 | 4.1:+22212840 | MS.gene26861:CDS |
| TTCATTGAACCATTTCTCTG+AGG | 0.619737 | 4.1:-22212637 | None:intergenic |
| AAAGAAGCAATTTCACACAA+AGG | 0.626707 | 4.1:+22213386 | MS.gene26861:CDS |
| GAGACTTGAAGCCAGAAATG+GGG | 0.636393 | 4.1:-22213592 | None:intergenic |
| AGACTTGAAGCCAGAAATGG+GGG | 0.646680 | 4.1:-22213591 | None:intergenic |
| ACTGTTTGAGTGTGCAACAG+GGG | 0.649035 | 4.1:+22212674 | MS.gene26861:CDS |
| GATGAAACAAGTGACGATCG+TGG | 0.671140 | 4.1:+22213446 | MS.gene26861:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTATATAACTTTTACTAATA+AGG | + | chr4.1:22213220-22213239 | MS.gene26861:intron | 10.0% |
| !!! | AATTGTTACTATTTCAATTT+TGG | + | chr4.1:22213035-22213054 | MS.gene26861:intron | 15.0% |
| !!! | TGACATTTAATTTAGTTATT+TGG | - | chr4.1:22213295-22213314 | None:intergenic | 15.0% |
| !!! | TTTTACTAATAAGGATAAAA+TGG | + | chr4.1:22213229-22213248 | MS.gene26861:intron | 15.0% |
| !! | AAGTTATATAACAAGATTGA+AGG | - | chr4.1:22213211-22213230 | None:intergenic | 20.0% |
| !! | AATGAAAATTTGAACTTTGT+TGG | + | chr4.1:22212948-22212967 | MS.gene26861:intron | 20.0% |
| !!! | ACAAATTAAGATGCATATTT+TGG | + | chr4.1:22213264-22213283 | MS.gene26861:intron | 20.0% |
| ! | TGTTAGACATTAAAATGTGT+AGG | + | chr4.1:22213177-22213196 | MS.gene26861:intron | 25.0% |
| !! | CAATTGTCTTTTATTACAGT+GGG | + | chr4.1:22213313-22213332 | MS.gene26861:intron | 25.0% |
| !! | TCAATTGTCTTTTATTACAG+TGG | + | chr4.1:22213312-22213331 | MS.gene26861:intron | 25.0% |
| !! | TCGTTGACTAAATTTAATTC+TGG | - | chr4.1:22213117-22213136 | None:intergenic | 25.0% |
| AAAGAAGCAATTTCACACAA+AGG | + | chr4.1:22213386-22213405 | MS.gene26861:CDS | 30.0% | |
| AAGAAAGAACAAAGCAAGAA+TGG | + | chr4.1:22213525-22213544 | MS.gene26861:CDS | 30.0% | |
| AGAAAGAACAAAGCAAGAAT+GGG | + | chr4.1:22213526-22213545 | MS.gene26861:CDS | 30.0% | |
| GAAAATTTGAACTTTGTTGG+TGG | + | chr4.1:22212951-22212970 | MS.gene26861:intron | 30.0% | |
| GAAATGGTTCAATGAAGATA+AGG | + | chr4.1:22212644-22212663 | MS.gene26861:CDS | 30.0% | |
| GATCAAAAACAGTGCATTTA+AGG | - | chr4.1:22212899-22212918 | None:intergenic | 30.0% | |
| GCTGTTCTTCAATATCTTTA+AGG | - | chr4.1:22213366-22213385 | None:intergenic | 30.0% | |
| ! | AAAACAGTGCATTTAAGGTT+AGG | - | chr4.1:22212894-22212913 | None:intergenic | 30.0% |
| ACTGCAATTGGACTATGATT+TGG | + | chr4.1:22213478-22213497 | MS.gene26861:CDS | 35.0% | |
| AGCAATTTCACACAAAGGAA+AGG | + | chr4.1:22213391-22213410 | MS.gene26861:CDS | 35.0% | |
| ATCCAAGTTCTTTAGACATG+TGG | + | chr4.1:22212610-22212629 | MS.gene26861:CDS | 35.0% | |
| ATTTCACACAAAGGAAAGGA+TGG | + | chr4.1:22213395-22213414 | MS.gene26861:CDS | 35.0% | |
| GAAAAACTCATATACGATGC+AGG | + | chr4.1:22213548-22213567 | MS.gene26861:CDS | 35.0% | |
| GAGTATGTTTCAAGATGCTA+TGG | + | chr4.1:22212743-22212762 | MS.gene26861:CDS | 35.0% | |
| GTCATTGTCAATATTGTGTC+CGG | - | chr4.1:22213087-22213106 | None:intergenic | 35.0% | |
| GTGATGCTTACAATGTTTCT+TGG | + | chr4.1:22213500-22213519 | MS.gene26861:CDS | 35.0% | |
| TACAAAATTACCCCCATTTC+TGG | + | chr4.1:22213581-22213600 | MS.gene26861:CDS | 35.0% | |
| TGGATTAACTGAACTGCAAT+TGG | + | chr4.1:22213466-22213485 | MS.gene26861:CDS | 35.0% | |
| TTCATTGAACCATTTCTCTG+AGG | - | chr4.1:22212640-22212659 | None:intergenic | 35.0% | |
| !! | CAATTTTGGTCTATGAAGCA+CGG | + | chr4.1:22213049-22213068 | MS.gene26861:intron | 35.0% |
| !!! | TGCTGATGCTGTTTTACTTA+AGG | + | chr4.1:22212998-22213017 | MS.gene26861:intron | 35.0% |
| AGAGACTTGAAGCCAGAAAT+GGG | - | chr4.1:22213596-22213615 | None:intergenic | 40.0% | |
| AGTAAAACAGCATCAGCAGA+AGG | - | chr4.1:22212996-22213015 | None:intergenic | 40.0% | |
| CTCCACATGTCTAAAGAACT+TGG | - | chr4.1:22212615-22212634 | None:intergenic | 40.0% | |
| GTAAAACAGCATCAGCAGAA+GGG | - | chr4.1:22212995-22213014 | None:intergenic | 40.0% | |
| GTTAAGTTACCAACAACCTG+TGG | - | chr4.1:22212924-22212943 | None:intergenic | 40.0% | |
| TTGGAGTCTCTTGTTGATGT+TGG | + | chr4.1:22212825-22212844 | MS.gene26861:CDS | 40.0% | |
| TTTGATCAACCACAGGTTGT+TGG | + | chr4.1:22212912-22212931 | MS.gene26861:intron | 40.0% | |
| !! | CAGGTTGTTGGTAACTTAAC+TGG | + | chr4.1:22212924-22212943 | MS.gene26861:intron | 40.0% |
| !!! | CACTGTTTTTGATCAACCAC+AGG | + | chr4.1:22212905-22212924 | MS.gene26861:CDS | 40.0% |
| ACACTGTTTGAGTGTGCAAC+AGG | + | chr4.1:22212672-22212691 | MS.gene26861:CDS | 45.0% | |
| ACTGTTTGAGTGTGCAACAG+GGG | + | chr4.1:22212674-22212693 | MS.gene26861:CDS | 45.0% | |
| AGACTTGAAGCCAGAAATGG+GGG | - | chr4.1:22213594-22213613 | None:intergenic | 45.0% | |
| CACTGTTTGAGTGTGCAACA+GGG | + | chr4.1:22212673-22212692 | MS.gene26861:CDS | 45.0% | |
| GAGACTTGAAGCCAGAAATG+GGG | - | chr4.1:22213595-22213614 | None:intergenic | 45.0% | |
| GAGAGACTTGAAGCCAGAAA+TGG | - | chr4.1:22213597-22213616 | None:intergenic | 45.0% | |
| GAGTCTCTTGTTGATGTTGG+AGG | + | chr4.1:22212828-22212847 | MS.gene26861:CDS | 45.0% | |
| GATGAAACAAGTGACGATCG+TGG | + | chr4.1:22213446-22213465 | MS.gene26861:CDS | 45.0% | |
| TCTCTTGTTGATGTTGGAGG+TGG | + | chr4.1:22212831-22212850 | MS.gene26861:CDS | 45.0% | |
| TGTGGAGTTCCTCAGAGAAA+TGG | + | chr4.1:22212628-22212647 | MS.gene26861:CDS | 45.0% | |
| ! | TACAGTGGGTTCTGCATGAT+TGG | + | chr4.1:22213327-22213346 | MS.gene26861:intron | 45.0% |
| AGAGAACAAACGCGTGTTCG+AGG | + | chr4.1:22212800-22212819 | MS.gene26861:CDS | 50.0% | |
| CGAACACGCGTTTGTTCTCT+TGG | - | chr4.1:22212800-22212819 | None:intergenic | 50.0% | |
| GAGAACAAACGCGTGTTCGA+GGG | + | chr4.1:22212801-22212820 | MS.gene26861:CDS | 50.0% | |
| GTTGATGTTGGAGGTGGTAC+TGG | + | chr4.1:22212837-22212856 | MS.gene26861:CDS | 50.0% | |
| ! | TGCAACAGGGGAGAGCTTTT+GGG | + | chr4.1:22212686-22212705 | MS.gene26861:CDS | 50.0% |
| CAAACGCGTGTTCGAGGGTT+TGG | + | chr4.1:22212806-22212825 | MS.gene26861:CDS | 55.0% | |
| ! | GATGTTGGAGGTGGTACTGG+TGG | + | chr4.1:22212840-22212859 | MS.gene26861:CDS | 55.0% |
| ! | GTGCAACAGGGGAGAGCTTT+TGG | + | chr4.1:22212685-22212704 | MS.gene26861:CDS | 55.0% |
| AGCACGGACACTGCACACAC+CGG | + | chr4.1:22213065-22213084 | MS.gene26861:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 22212601 | 22213634 | 22212601 | ID=MS.gene26861 |
| chr4.1 | mRNA | 22212601 | 22213634 | 22212601 | ID=MS.gene26861.t1;Parent=MS.gene26861 |
| chr4.1 | exon | 22212601 | 22212926 | 22212601 | ID=MS.gene26861.t1.exon1;Parent=MS.gene26861.t1 |
| chr4.1 | CDS | 22212601 | 22212926 | 22212601 | ID=cds.MS.gene26861.t1;Parent=MS.gene26861.t1 |
| chr4.1 | exon | 22213332 | 22213634 | 22213332 | ID=MS.gene26861.t1.exon2;Parent=MS.gene26861.t1 |
| chr4.1 | CDS | 22213332 | 22213634 | 22213332 | ID=cds.MS.gene26861.t1;Parent=MS.gene26861.t1 |
| Gene Sequence |
| Protein sequence |
