AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene26874


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene26874.t1 MTR_7g062720 40.541 148 80 3 1 146 1 142 5.04e-31 115
MS.gene26874.t1 MTR_4g037355 40.441 136 61 4 155 290 1 116 1.14e-21 89.7
MS.gene26874.t1 MTR_5g061150 35.354 99 64 0 52 150 38 136 4.24e-18 80.1
MS.gene26874.t1 MTR_4g112730 57.971 69 28 1 143 211 2 69 8.40e-18 77.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 60 sgRNAs with CRISPR-Local

Find 90 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGATCAATGGAACAAATTA+AGG 0.196151 1.4:-80091847 MS.gene26874:CDS
AGTCTTATTGCCCTCATTAA+TGG 0.201947 1.4:-80091867 MS.gene26874:CDS
AAAGTTGTGGCGTTTATTTC+AGG 0.203868 1.4:+80091551 None:intergenic
CAAAAGATCCCAAGATCTTA+TGG 0.237847 1.4:-80091925 MS.gene26874:CDS
GATTCTTGAGGGAGAAGATT+TGG 0.253340 1.4:-80092044 MS.gene26874:CDS
GACAAAATTAAGGCAAGATT+TGG 0.257188 1.4:-80091902 MS.gene26874:CDS
CTCAATTAAGTTGTTGTTCC+AGG 0.274617 1.4:+80092093 None:intergenic
CACGACCTCCACCACGAAAA+TGG 0.277262 1.4:+80091435 None:intergenic
GATCTTATGGGACAAAATTA+AGG 0.310971 1.4:-80091912 MS.gene26874:CDS
ATTTACTGAATACTCTGATT+TGG 0.313227 1.4:-80091657 MS.gene26874:CDS
ATTCTTGAGGGAGAAGATTT+GGG 0.333151 1.4:-80092043 MS.gene26874:CDS
AGACAGGTAGTGTCATTCTT+TGG 0.342502 1.4:+80091179 None:intergenic
GGGAGATGTCCCAGAGGAAT+TGG 0.345733 1.4:-80092023 MS.gene26874:CDS
GAAGAGGTTATATGAATTAC+AGG 0.363099 1.4:-80091412 MS.gene26874:CDS
GATTTCTAGAGGGACCTTCC+TGG 0.373636 1.4:+80091336 None:intergenic
CATAGCCACCACGTCCTCTT+TGG 0.374275 1.4:+80091501 None:intergenic
CGTGGTGGCTATGCTCGTTT+TGG 0.395091 1.4:-80091491 MS.gene26874:CDS
ACAAAATTAAGGCAAGATTT+GGG 0.416566 1.4:-80091901 MS.gene26874:CDS
TATAACTGAAAAGGAAAAGT+TGG 0.422757 1.4:-80091744 MS.gene26874:CDS
TGCCCTCATTAATGGATCAA+TGG 0.432677 1.4:-80091859 MS.gene26874:CDS
CGTGGTGGAGGTCGTGGAAG+AGG 0.449888 1.4:-80091428 MS.gene26874:CDS
AAAAGATCCCAAGATCTTAT+GGG 0.458748 1.4:-80091924 MS.gene26874:CDS
AACCATCAGTTGCGTCCTAC+AGG 0.459311 1.4:-80091587 MS.gene26874:CDS
TGGCAAAACTATAACTGAAA+AGG 0.467052 1.4:-80091753 MS.gene26874:CDS
ATCTGTGGATGGAAAGGAAA+GGG 0.491819 1.4:-80091225 MS.gene26874:CDS
ATCAAGCACCACATTGATGA+TGG 0.492315 1.4:-80091971 MS.gene26874:CDS
TGTTATAGATGCGGAAAGAA+AGG 0.495634 1.4:-80091302 MS.gene26874:CDS
CTGGCGACAACTACATAACC+TGG 0.502097 1.4:-80092111 MS.gene26874:CDS
GTTCCATTGATCCATTAATG+AGG 0.509644 1.4:+80091856 None:intergenic
ATGCGGAAAGAAAGGCCATT+GGG 0.516896 1.4:-80091294 MS.gene26874:CDS
TTTGATTGGTCATATTTGAG+AGG 0.522357 1.4:+80091389 None:intergenic
CAAAGAATCTGTGGATGGAA+AGG 0.532926 1.4:-80091231 MS.gene26874:CDS
TTCCATTGATCCATTAATGA+GGG 0.533283 1.4:+80091857 None:intergenic
CGTGGACGAGGTAGAAATCA+TGG 0.535808 1.4:-80091464 MS.gene26874:CDS
GGACTCGATAAGATTCTTGA+GGG 0.539399 1.4:-80092055 MS.gene26874:CDS
CCCTCTAGAAATCATGATGA+TGG 0.541875 1.4:-80091326 MS.gene26874:CDS
TTCCTGTAGGACGCAACTGA+TGG 0.562606 1.4:+80091585 None:intergenic
AAAATCAGCAGCCTCAAGAC+AGG 0.562896 1.4:+80091163 None:intergenic
AATTGAGTATCTTGCATGTG+AGG 0.565013 1.4:-80092077 MS.gene26874:CDS
GCCATTGGGCTAAAACATGC+AGG 0.566864 1.4:-80091280 MS.gene26874:CDS
ATTGAGTATCTTGCATGTGA+GGG 0.575966 1.4:-80092076 MS.gene26874:CDS
TTTGGTTGCAGCCCTTCTAG+TGG 0.582793 1.4:-80091639 MS.gene26874:CDS
GATGCGGAAAGAAAGGCCAT+TGG 0.583841 1.4:-80091295 MS.gene26874:CDS
TTGTGCAATAATTTGATGCA+TGG 0.588693 1.4:+80091787 None:intergenic
TAAAAGATACAAAGAATCTG+TGG 0.594054 1.4:-80091240 MS.gene26874:CDS
AGATACAAAGAATCTGTGGA+TGG 0.602731 1.4:-80091236 MS.gene26874:CDS
GGGACTCGATAAGATTCTTG+AGG 0.606176 1.4:-80092056 MS.gene26874:CDS
ACGCCCTTGATTAAAAGTTG+TGG 0.608515 1.4:+80091538 None:intergenic
GCAATATCACTTGAGATGCA+TGG 0.608661 1.4:+80091702 None:intergenic
TATAATCGCCAAAGAGGACG+TGG 0.608957 1.4:-80091509 MS.gene26874:CDS
ACCATCATCATGATTTCTAG+AGG 0.616492 1.4:+80091325 None:intergenic
GGATACGACATTGTTCCTGT+AGG 0.627452 1.4:+80091572 None:intergenic
GGTGGTTATAATCGCCAAAG+AGG 0.642086 1.4:-80091515 MS.gene26874:CDS
CCATCATCATGATTTCTAGA+GGG 0.647682 1.4:+80091326 None:intergenic
GAATGACACTACCTGTCTTG+AGG 0.649563 1.4:-80091174 MS.gene26874:CDS
AATCTGTGGATGGAAAGGAA+AGG 0.653361 1.4:-80091226 MS.gene26874:CDS
GATGATGGTTGTTATAGATG+CGG 0.696569 1.4:-80091311 MS.gene26874:CDS
AATCGCCAAAGAGGACGTGG+TGG 0.698905 1.4:-80091506 MS.gene26874:CDS
TTTGTAATCCATCATCAATG+TGG 0.715751 1.4:+80091963 None:intergenic
AGATTTGGGAGATGTCCCAG+AGG 0.741186 1.4:-80092029 MS.gene26874:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTCAAAATTTTAAGCATAAC+TGG - chr1.4:80091157-80091176 MS.gene26874:CDS 20.0%
! ACAAAATTAAGGCAAGATTT+GGG - chr1.4:80091386-80091405 MS.gene26874:CDS 25.0%
! ATTTACTGAATACTCTGATT+TGG - chr1.4:80091630-80091649 MS.gene26874:CDS 25.0%
! CAATCAAACAAAAATCATCA+GGG - chr1.4:80091913-80091932 MS.gene26874:CDS 25.0%
! TAAAAGATACAAAGAATCTG+TGG - chr1.4:80092047-80092066 MS.gene26874:CDS 25.0%
! TATAACTGAAAAGGAAAAGT+TGG - chr1.4:80091543-80091562 MS.gene26874:CDS 25.0%
!! ATTGCACAATTAGAATTTTG+TGG - chr1.4:80091514-80091533 MS.gene26874:CDS 25.0%
!! TATCTTTTACATAGATACTC+TGG + chr1.4:80092036-80092055 None:intergenic 25.0%
!!! CAACAACAGTTTTATAATCT+TGG + chr1.4:80091468-80091487 None:intergenic 25.0%
!!! TGTTGAAGGAGATTTTAATA+TGG - chr1.4:80092146-80092165 MS.gene26874:CDS 25.0%
AAAAGATCCCAAGATCTTAT+GGG - chr1.4:80091363-80091382 MS.gene26874:CDS 30.0%
AACAAAAATCATCAGGGTAA+AGG - chr1.4:80091919-80091938 MS.gene26874:CDS 30.0%
CCAATCAAACAAAAATCATC+AGG - chr1.4:80091912-80091931 MS.gene26874:CDS 30.0%
GAAGAGGTTATATGAATTAC+AGG - chr1.4:80091875-80091894 MS.gene26874:CDS 30.0%
GACAAAATTAAGGCAAGATT+TGG - chr1.4:80091385-80091404 MS.gene26874:CDS 30.0%
GATCTTATGGGACAAAATTA+AGG - chr1.4:80091375-80091394 MS.gene26874:CDS 30.0%
TGGATCAATGGAACAAATTA+AGG - chr1.4:80091440-80091459 MS.gene26874:CDS 30.0%
TGGCAAAACTATAACTGAAA+AGG - chr1.4:80091534-80091553 MS.gene26874:CDS 30.0%
TTCCATTGATCCATTAATGA+GGG + chr1.4:80091433-80091452 None:intergenic 30.0%
TTGTGCAATAATTTGATGCA+TGG + chr1.4:80091503-80091522 None:intergenic 30.0%
TTTGTAATCCATCATCAATG+TGG + chr1.4:80091327-80091346 None:intergenic 30.0%
! AATTTTGTCCCATAAGATCT+TGG + chr1.4:80091374-80091393 None:intergenic 30.0%
! ATTTTGTCCCATAAGATCTT+GGG + chr1.4:80091373-80091392 None:intergenic 30.0%
! TTTGATTGGTCATATTTGAG+AGG + chr1.4:80091901-80091920 None:intergenic 30.0%
!! CCTGATGATTTTTGTTTGAT+TGG + chr1.4:80091915-80091934 None:intergenic 30.0%
AAAGGTAAAAACATCCAGGA+AGG - chr1.4:80091937-80091956 MS.gene26874:CDS 35.0%
AAAGTTGTGGCGTTTATTTC+AGG + chr1.4:80091739-80091758 None:intergenic 35.0%
AATTGAGTATCTTGCATGTG+AGG - chr1.4:80091210-80091229 MS.gene26874:CDS 35.0%
AGATACAAAGAATCTGTGGA+TGG - chr1.4:80092051-80092070 MS.gene26874:CDS 35.0%
ATTCTTGAGGGAGAAGATTT+GGG - chr1.4:80091244-80091263 MS.gene26874:CDS 35.0%
ATTGAGTATCTTGCATGTGA+GGG - chr1.4:80091211-80091230 MS.gene26874:CDS 35.0%
CAAAAGATCCCAAGATCTTA+TGG - chr1.4:80091362-80091381 MS.gene26874:CDS 35.0%
GTTCCATTGATCCATTAATG+AGG + chr1.4:80091434-80091453 None:intergenic 35.0%
TGTTATAGATGCGGAAAGAA+AGG - chr1.4:80091985-80092004 MS.gene26874:CDS 35.0%
! AACGCCACAACTTTTAATCA+AGG - chr1.4:80091745-80091764 MS.gene26874:CDS 35.0%
! ACCATCATCATGATTTCTAG+AGG + chr1.4:80091965-80091984 None:intergenic 35.0%
! ACGCCACAACTTTTAATCAA+GGG - chr1.4:80091746-80091765 MS.gene26874:CDS 35.0%
! ATTTTGTTCAGCCACTAGAA+GGG + chr1.4:80091662-80091681 None:intergenic 35.0%
! CCATCATCATGATTTCTAGA+GGG + chr1.4:80091964-80091983 None:intergenic 35.0%
! CTCAATTAAGTTGTTGTTCC+AGG + chr1.4:80091197-80091216 None:intergenic 35.0%
! GATGATGGTTGTTATAGATG+CGG - chr1.4:80091976-80091995 MS.gene26874:CDS 35.0%
! TATTTTGTTCAGCCACTAGA+AGG + chr1.4:80091663-80091682 None:intergenic 35.0%
! TTTTCTTTGCCAATTCCTCT+GGG + chr1.4:80091276-80091295 None:intergenic 35.0%
! TTTTTCTTTGCCAATTCCTC+TGG + chr1.4:80091277-80091296 None:intergenic 35.0%
!! AGTCTTATTGCCCTCATTAA+TGG - chr1.4:80091420-80091439 MS.gene26874:CDS 35.0%
AATCTGTGGATGGAAAGGAA+AGG - chr1.4:80092061-80092080 MS.gene26874:CDS 40.0%
ACGCCCTTGATTAAAAGTTG+TGG + chr1.4:80091752-80091771 None:intergenic 40.0%
ATCAAGCACCACATTGATGA+TGG - chr1.4:80091316-80091335 MS.gene26874:CDS 40.0%
ATCTGTGGATGGAAAGGAAA+GGG - chr1.4:80092062-80092081 MS.gene26874:CDS 40.0%
CAAAGAATCTGTGGATGGAA+AGG - chr1.4:80092056-80092075 MS.gene26874:CDS 40.0%
GATTCTTGAGGGAGAAGATT+TGG - chr1.4:80091243-80091262 MS.gene26874:CDS 40.0%
GCAATATCACTTGAGATGCA+TGG + chr1.4:80091588-80091607 None:intergenic 40.0%
GGACTCGATAAGATTCTTGA+GGG - chr1.4:80091232-80091251 MS.gene26874:CDS 40.0%
GGGTAAAGGTAAAAACATCC+AGG - chr1.4:80091933-80091952 MS.gene26874:CDS 40.0%
! ACAACTTTTAATCAAGGGCG+TGG - chr1.4:80091751-80091770 MS.gene26874:CDS 40.0%
! AGACAGGTAGTGTCATTCTT+TGG + chr1.4:80092111-80092130 None:intergenic 40.0%
! CCCTCTAGAAATCATGATGA+TGG - chr1.4:80091961-80091980 MS.gene26874:CDS 40.0%
! TGCCCTCATTAATGGATCAA+TGG - chr1.4:80091428-80091447 MS.gene26874:CDS 40.0%
AAAATCAGCAGCCTCAAGAC+AGG + chr1.4:80092127-80092146 None:intergenic 45.0%
ATGCGGAAAGAAAGGCCATT+GGG - chr1.4:80091993-80092012 MS.gene26874:CDS 45.0%
GAATGACACTACCTGTCTTG+AGG - chr1.4:80092113-80092132 MS.gene26874:CDS 45.0%
GGATACGACATTGTTCCTGT+AGG + chr1.4:80091718-80091737 None:intergenic 45.0%
GGGACTCGATAAGATTCTTG+AGG - chr1.4:80091231-80091250 MS.gene26874:CDS 45.0%
GGTGGTTATAATCGCCAAAG+AGG - chr1.4:80091772-80091791 MS.gene26874:CDS 45.0%
TATAATCGCCAAAGAGGACG+TGG - chr1.4:80091778-80091797 MS.gene26874:CDS 45.0%
! CATGGTCGAAACCATTTTCG+TGG - chr1.4:80091841-80091860 MS.gene26874:CDS 45.0%
!! ACTTTTAATCAAGGGCGTGG+TGG - chr1.4:80091754-80091773 MS.gene26874:CDS 45.0%
!!! GAGGCTGCTGATTTTGTTGA+AGG - chr1.4:80092132-80092151 MS.gene26874:CDS 45.0%
!!! TCCTGCATGTTTTAGCCCAA+TGG + chr1.4:80092011-80092030 None:intergenic 45.0%
AACCATCAGTTGCGTCCTAC+AGG - chr1.4:80091700-80091719 MS.gene26874:CDS 50.0%
AGATTTGGGAGATGTCCCAG+AGG - chr1.4:80091258-80091277 MS.gene26874:CDS 50.0%
CGTGGACGAGGTAGAAATCA+TGG - chr1.4:80091823-80091842 MS.gene26874:CDS 50.0%
CTGGCGACAACTACATAACC+TGG - chr1.4:80091176-80091195 MS.gene26874:CDS 50.0%
GATGCGGAAAGAAAGGCCAT+TGG - chr1.4:80091992-80092011 MS.gene26874:CDS 50.0%
GCCATTGGGCTAAAACATGC+AGG - chr1.4:80092007-80092026 MS.gene26874:CDS 50.0%
TTCCTGTAGGACGCAACTGA+TGG + chr1.4:80091705-80091724 None:intergenic 50.0%
TTTGGTTGCAGCCCTTCTAG+TGG - chr1.4:80091648-80091667 MS.gene26874:CDS 50.0%
! CGAAACCATTTTCGTGGTGG+AGG - chr1.4:80091847-80091866 MS.gene26874:CDS 50.0%
! GATTTCTAGAGGGACCTTCC+TGG + chr1.4:80091954-80091973 None:intergenic 50.0%
! GGTCGAAACCATTTTCGTGG+TGG - chr1.4:80091844-80091863 MS.gene26874:CDS 50.0%
!! TATGCTCGTTTTGGTGGTCG+TGG - chr1.4:80091805-80091824 MS.gene26874:CDS 50.0%
AATCGCCAAAGAGGACGTGG+TGG - chr1.4:80091781-80091800 MS.gene26874:CDS 55.0%
CACGACCTCCACCACGAAAA+TGG + chr1.4:80091855-80091874 None:intergenic 55.0%
CATAGCCACCACGTCCTCTT+TGG + chr1.4:80091789-80091808 None:intergenic 55.0%
GGGAGATGTCCCAGAGGAAT+TGG - chr1.4:80091264-80091283 MS.gene26874:CDS 55.0%
! CGTGGTGGCTATGCTCGTTT+TGG - chr1.4:80091796-80091815 MS.gene26874:CDS 55.0%
! GGTGGCTATGCTCGTTTTGG+TGG - chr1.4:80091799-80091818 MS.gene26874:CDS 55.0%
!! CATTTTCGTGGTGGAGGTCG+TGG - chr1.4:80091853-80091872 MS.gene26874:CDS 55.0%
!! CGTTTTGGTGGTCGTGGACG+AGG - chr1.4:80091811-80091830 MS.gene26874:CDS 60.0%
! CGTGGTGGAGGTCGTGGAAG+AGG - chr1.4:80091859-80091878 MS.gene26874:CDS 65.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 80091136 80092173 80091136 ID=MS.gene26874
chr1.4 mRNA 80091136 80092173 80091136 ID=MS.gene26874.t1;Parent=MS.gene26874
chr1.4 exon 80091136 80092173 80091136 ID=MS.gene26874.t1.exon1;Parent=MS.gene26874.t1
chr1.4 CDS 80091136 80092173 80091136 ID=cds.MS.gene26874.t1;Parent=MS.gene26874.t1
Gene Sequence

>MS.gene26874

ATGTCAAATGTTAAGCATCAATTCAAAATTTTAAGCATAACTGGCGACAACTACATAACCTGGAACAACAACTTAATTGAGTATCTTGCATGTGAGGGACTCGATAAGATTCTTGAGGGAGAAGATTTGGGAGATGTCCCAGAGGAATTGGCAAAGAAAAAATCAAAAGTAAATCGTATAATCAAGCACCACATTGATGATGGATTACAAACAGAGTATTCAAATGCAAAAGATCCCAAGATCTTATGGGACAAAATTAAGGCAAGATTTGGGCATCAGAAGAAAGTCTTATTGCCCTCATTAATGGATCAATGGAACAAATTAAGGTTCCAAGATTATAAAACTGTTGTTGCATATAACTCTGCCATGCATCAAATTATTGCACAATTAGAATTTTGTGGCAAAACTATAACTGAAAAGGAAAAGTTGGAGAAAACATTCTCAACTTTCCATGCATCTCAAGTGATATTGCAACAACAATATAGAATGAGAGAATTTACTGAATACTCTGATTTGGTTGCAGCCCTTCTAGTGGCTGAACAAAATAACGAGCTGCTCATTAAAAACCATCAGTTGCGTCCTACAGGAACAATGTCGTATCCTGAAATAAACGCCACAACTTTTAATCAAGGGCGTGGTGGTTATAATCGCCAAAGAGGACGTGGTGGCTATGCTCGTTTTGGTGGTCGTGGACGAGGTAGAAATCATGGTCGAAACCATTTTCGTGGTGGAGGTCGTGGAAGAGGTTATATGAATTACAGGCCTCTCAAATATGACCAATCAAACAAAAATCATCAGGGTAAAGGTAAAAACATCCAGGAAGGTCCCTCTAGAAATCATGATGATGGTTGTTATAGATGCGGAAAGAAAGGCCATTGGGCTAAAACATGCAGGACACCAGAGTATCTATGTAAAAGATACAAAGAATCTGTGGATGGAAAGGAAAGGGAAGTTAATTTTAATGAAATTGAACCAAAGAATGACACTACCTGTCTTGAGGCTGCTGATTTTGTTGAAGGAGATTTTAATATGGATTAA

Protein sequence

>MS.gene26874.t1

MSNVKHQFKILSITGDNYITWNNNLIEYLACEGLDKILEGEDLGDVPEELAKKKSKVNRIIKHHIDDGLQTEYSNAKDPKILWDKIKARFGHQKKVLLPSLMDQWNKLRFQDYKTVVAYNSAMHQIIAQLEFCGKTITEKEKLEKTFSTFHASQVILQQQYRMREFTEYSDLVAALLVAEQNNELLIKNHQLRPTGTMSYPEINATTFNQGRGGYNRQRGRGGYARFGGRGRGRNHGRNHFRGGGRGRGYMNYRPLKYDQSNKNHQGKGKNIQEGPSRNHDDGCYRCGKKGHWAKTCRTPEYLCKRYKESVDGKEREVNFNEIEPKNDTTCLEAADFVEGDFNMD