AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene27546

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene27546.t1 MTR_7g074650 99.448 362 2 0 365 726 444 805 0.0 761
MS.gene27546.t1 MTR_7g074650 92.329 365 18 5 3 364 2 359 4.23e-170 508
MS.gene27546.t1 MTR_4g076020 59.322 354 127 4 365 714 90 430 1.34e-145 432
MS.gene27546.t1 MTR_1g069725 54.131 351 153 5 366 712 116 462 4.87e-127 386
MS.gene27546.t1 MTR_7g027190 41.129 372 192 6 365 713 306 673 2.86e-90 296
MS.gene27546.t1 MTR_1g029420 37.736 371 216 6 357 713 223 592 2.99e-65 227
MS.gene27546.t1 MTR_3g065980 36.340 377 202 11 365 715 178 542 1.18e-61 216
MS.gene27546.t1 MTR_4g102790 35.200 375 209 10 365 714 298 663 6.24e-61 217
MS.gene27546.t1 MTR_3g089055 35.580 371 224 6 357 713 201 570 9.15e-61 214
MS.gene27546.t1 MTR_3g089055 35.580 371 224 6 357 713 196 565 1.61e-60 214
MS.gene27546.t1 MTR_2g082090 37.389 337 196 6 390 712 243 578 2.35e-59 211
MS.gene27546.t1 MTR_5g094450 35.215 372 222 8 360 713 162 532 1.16e-57 205
MS.gene27546.t1 MTR_5g097480 34.153 366 220 7 365 713 183 544 2.92e-56 201
MS.gene27546.t1 MTR_3g056110 33.333 363 227 6 365 713 181 542 4.79e-54 195
MS.gene27546.t1 MTR_4g133660 35.608 337 202 6 390 712 207 542 1.43e-53 194
MS.gene27546.t1 MTR_8g442410 34.332 367 221 7 365 713 161 525 5.77e-53 192
MS.gene27546.t1 MTR_5g058860 35.904 376 208 12 368 712 123 496 1.09e-52 191
MS.gene27546.t1 MTR_4g077760 35.988 339 195 10 390 713 224 555 1.67e-52 191
MS.gene27546.t1 MTR_1g096030 32.546 381 216 12 366 713 87 459 1.91e-51 186
MS.gene27546.t1 MTR_3g072710 36.267 375 203 14 368 712 131 499 3.54e-50 184
MS.gene27546.t1 MTR_2g097410 31.421 366 235 6 365 714 377 742 5.72e-50 187
MS.gene27546.t1 MTR_3g022830 29.167 408 228 8 365 713 31 436 6.51e-50 181
MS.gene27546.t1 MTR_2g026250 30.256 390 251 8 338 713 214 596 1.21e-49 184
MS.gene27546.t1 MTR_4g104020 31.250 368 232 8 365 713 155 520 7.88e-48 177
MS.gene27546.t1 MTR_7g062120 30.220 364 240 6 365 714 378 741 1.03e-47 181
MS.gene27546.t1 MTR_4g064120 29.539 369 240 7 365 714 260 627 3.02e-46 175
MS.gene27546.t1 MTR_4g064160 30.495 364 239 5 365 714 322 685 6.54e-46 174
MS.gene27546.t1 MTR_4g064200 30.328 366 235 8 365 714 290 651 8.44e-46 174
MS.gene27546.t1 MTR_4g064150 30.163 368 239 7 365 714 367 734 1.20e-45 174
MS.gene27546.t1 MTR_4g064180 29.810 369 239 8 365 714 260 627 7.42e-45 171
MS.gene27546.t1 MTR_2g097310 31.436 369 227 10 365 714 278 639 1.15e-43 167
MS.gene27546.t1 MTR_2g097350 30.854 363 237 8 365 714 280 641 1.59e-43 167
MS.gene27546.t1 MTR_2g097467 28.846 364 242 6 365 714 295 655 1.33e-42 164
MS.gene27546.t1 MTR_2g097463 29.121 364 241 6 365 714 296 656 1.68e-41 161
MS.gene27546.t1 MTR_4g097080 28.378 370 239 11 366 714 140 504 2.05e-41 159
MS.gene27546.t1 MTR_2g097390 30.579 363 238 8 365 714 281 642 1.55e-40 158
MS.gene27546.t1 MTR_2g097473 28.571 364 243 7 365 714 295 655 2.15e-39 155
MS.gene27546.t1 MTR_7g109580 31.165 369 239 10 358 713 188 554 2.33e-39 153
MS.gene27546.t1 MTR_2g097380 30.495 364 208 11 365 714 230 562 2.50e-39 154
MS.gene27546.t1 MTR_2g034280 31.351 370 230 11 365 713 209 575 4.11e-39 153
MS.gene27546.t1 MTR_5g015490 28.191 376 238 13 366 714 121 491 1.50e-38 150
MS.gene27546.t1 MTR_5g009080 40.957 188 106 2 367 551 64 249 2.84e-38 149
MS.gene27546.t1 MTR_5g009080 38.255 149 87 3 567 713 333 478 1.18e-22 102
MS.gene27546.t1 MTR_2g034260 31.099 373 229 12 365 713 216 584 1.19e-37 149
MS.gene27546.t1 MTR_2g034250 30.108 372 235 12 365 713 216 585 1.49e-36 145
MS.gene27546.t1 MTR_4g076140 40.107 187 109 2 367 551 56 241 2.28e-35 140
MS.gene27546.t1 MTR_4g076140 37.584 149 88 3 567 713 324 469 2.74e-20 95.1
MS.gene27546.t1 MTR_2g099110 26.649 379 241 9 366 714 81 452 5.17e-35 139
MS.gene27546.t1 MTR_8g093070 28.698 338 206 12 395 714 177 497 2.09e-32 132
MS.gene27546.t1 MTR_4g026485 26.178 382 251 8 349 713 257 624 8.27e-27 116
MS.gene27546.t1 MTR_7g057230 27.273 374 243 11 358 712 293 656 8.95e-27 116
MS.gene27546.t1 MTR_0092s0100 27.446 368 243 8 359 713 375 731 1.91e-26 115
MS.gene27546.t1 MTR_0092s0100 27.446 368 243 8 359 713 372 728 1.94e-26 115
MS.gene27546.t1 MTR_1g086970 25.187 401 264 13 344 713 83 478 2.42e-26 113
MS.gene27546.t1 MTR_8g077940 27.500 360 232 13 371 713 194 541 6.19e-24 107
MS.gene27546.t1 MTR_6g047750 28.940 349 206 13 392 713 329 662 3.94e-22 102
MS.gene27546.t1 MTR_2g089100 21.939 392 257 11 366 714 59 444 6.25e-20 94.0
MS.gene27546.t1 MTR_5g015950 23.114 411 249 14 366 714 42 447 1.93e-18 89.4
MS.gene27546.t1 MTR_3g053270 23.114 411 249 14 366 714 42 447 1.93e-18 89.4
MS.gene27546.t1 MTR_8g020840 24.479 384 241 13 366 713 178 548 1.08e-17 87.4
MS.gene27546.t1 MTR_4g095500 23.415 410 247 14 366 714 66 469 2.43e-15 79.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene27546.t1 AT3G54220 64.273 571 129 8 198 714 102 651 0.0 709
MS.gene27546.t1 AT5G41920 57.222 360 142 6 365 716 49 404 8.79e-137 409
MS.gene27546.t1 AT1G14920 37.534 365 199 11 365 713 178 529 7.47e-63 219
MS.gene27546.t1 AT1G66350 37.255 357 205 10 365 713 161 506 1.12e-62 218
MS.gene27546.t1 AT3G03450 37.500 368 200 11 365 713 189 545 1.17e-61 216
MS.gene27546.t1 AT1G63100 35.580 371 216 9 365 712 258 628 1.57e-60 215
MS.gene27546.t1 AT1G63100 35.580 371 216 9 365 712 283 653 4.07e-60 214
MS.gene27546.t1 AT1G50600 35.598 368 222 6 360 713 160 526 8.21e-60 211
MS.gene27546.t1 AT2G01570 36.986 365 201 11 365 713 230 581 1.15e-59 212
MS.gene27546.t1 AT1G50600 35.598 368 222 6 360 713 231 597 1.63e-59 211
MS.gene27546.t1 AT1G50600 35.598 368 222 6 360 713 235 601 1.74e-59 211
MS.gene27546.t1 AT2G04890 35.278 360 217 7 365 713 59 413 4.15e-59 206
MS.gene27546.t1 AT5G17490 34.406 404 236 12 328 713 124 516 4.63e-58 206
MS.gene27546.t1 AT5G66770 36.066 366 207 12 367 713 227 584 4.51e-55 199
MS.gene27546.t1 AT5G48150 33.693 371 216 9 365 713 128 490 7.18e-52 188
MS.gene27546.t1 AT5G48150 33.693 371 216 9 365 713 128 490 7.18e-52 188
MS.gene27546.t1 AT5G48150 33.693 371 216 9 365 713 128 490 7.18e-52 188
MS.gene27546.t1 AT5G48150 33.693 371 216 9 365 713 128 490 7.18e-52 188
MS.gene27546.t1 AT1G21450 34.711 363 221 5 366 713 232 593 7.86e-52 190
MS.gene27546.t1 AT4G17230 34.153 366 220 7 366 714 165 526 2.58e-51 187
MS.gene27546.t1 AT4G17230 34.153 366 220 7 366 714 165 526 2.58e-51 187
MS.gene27546.t1 AT5G59450 28.191 376 251 8 365 721 233 608 4.28e-46 174
MS.gene27546.t1 AT1G07530 30.959 365 237 6 365 714 402 766 1.89e-45 174
MS.gene27546.t1 AT2G37650 29.945 364 239 6 365 714 341 702 1.23e-44 171
MS.gene27546.t1 AT2G37650 29.945 364 239 6 365 714 353 714 1.60e-44 171
MS.gene27546.t1 AT3G50650 32.692 364 220 10 369 713 185 542 6.51e-44 166
MS.gene27546.t1 AT1G55580 32.576 396 216 12 367 713 52 445 9.82e-43 161
MS.gene27546.t1 AT2G29060 30.352 369 236 9 365 714 290 656 1.13e-40 159
MS.gene27546.t1 AT3G46600 29.235 366 240 8 365 714 10 372 1.57e-40 153
MS.gene27546.t1 AT2G29060 30.352 369 236 9 365 714 327 693 1.60e-40 159
MS.gene27546.t1 AT3G46600 29.235 366 240 8 365 714 218 580 8.83e-40 155
MS.gene27546.t1 AT3G46600 29.235 366 240 8 365 714 186 548 1.12e-39 154
MS.gene27546.t1 AT4G37650 27.822 381 240 10 366 714 153 530 1.23e-38 151
MS.gene27546.t1 AT4G08250 30.337 356 230 8 373 712 123 476 6.43e-38 148
MS.gene27546.t1 AT2G29065 28.000 375 242 10 365 714 258 629 4.44e-36 145
MS.gene27546.t1 AT1G07520 28.342 374 241 9 365 714 324 694 7.75e-36 144
MS.gene27546.t1 AT1G07520 28.342 374 241 9 365 714 325 695 8.46e-36 144
MS.gene27546.t1 AT1G07520 28.342 374 241 9 365 714 160 530 9.85e-36 142
MS.gene27546.t1 AT1G07520 28.342 374 241 9 365 714 160 530 9.85e-36 142
MS.gene27546.t1 AT1G50420 38.830 188 112 1 367 551 65 252 2.20e-34 137
MS.gene27546.t1 AT1G50420 34.416 154 96 3 562 713 329 479 3.18e-19 92.0
MS.gene27546.t1 AT5G52510 28.571 378 228 11 366 713 275 640 3.71e-30 127
MS.gene27546.t1 AT4G36710 26.776 366 240 11 365 713 131 485 6.52e-25 109
MS.gene27546.t1 AT3G60630 30.351 313 170 11 431 713 328 622 8.53e-25 110
MS.gene27546.t1 AT4G00150 28.863 343 210 13 384 713 233 554 1.64e-23 105
MS.gene27546.t1 AT2G45160 29.508 305 180 10 431 713 348 639 6.08e-22 101
MS.gene27546.t1 AT3G49950 25.858 379 245 15 366 714 37 409 7.11e-19 90.1
MS.gene27546.t1 AT3G13840 25.991 454 279 18 295 713 79 510 9.21e-19 90.9

Find 112 sgRNAs with CRISPR-Local

Find 166 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATCTTTCCAACCAAGTTTA+AGG 0.116743 7.2:-30758713 None:intergenic
ATCAAAACATGTTTCCTAAT+TGG 0.117353 7.2:+30756863 MS.gene27546:CDS
TTACCTTCATCCACAGAAAA+AGG 0.236542 7.2:+30756070 MS.gene27546:CDS
TCAAAACATGTTTCCTAATT+GGG 0.250183 7.2:+30756864 MS.gene27546:CDS
GTGAGATAAAGTTTCATAAT+TGG 0.256096 7.2:+30758565 MS.gene27546:CDS
GCTGCTTGTGCTTTGTTCAA+TGG 0.258459 7.2:+30755800 MS.gene27546:CDS
ACAGTTACTGTTTCTGTTGC+TGG 0.272831 7.2:-30756443 None:intergenic
TCCACTACTGTTACCACTTT+AGG 0.285917 7.2:-30758354 None:intergenic
TATACTTTGGTTGAGGATAA+TGG 0.311713 7.2:+30758687 MS.gene27546:CDS
ATTTGTGGCACACCCCAATT+AGG 0.312254 7.2:-30756877 None:intergenic
CATTCTCTATATGATGTTAC+TGG 0.333927 7.2:+30757711 MS.gene27546:CDS
GAGCAATGCAGGTTCATTCT+TGG 0.338861 7.2:+30758386 MS.gene27546:CDS
TCGCAGCTTTCAACGCCTTT+CGG 0.347062 7.2:+30757192 MS.gene27546:CDS
TTTCCAACTTTCTCAGCAAC+AGG 0.359463 7.2:-30757627 None:intergenic
TCAGCATTATTTGATTCTCT+TGG 0.361167 7.2:+30758438 MS.gene27546:CDS
TTTATCTCACCACTCCTTGA+TGG 0.363245 7.2:-30758552 None:intergenic
ACACAAGCTAGTCTTCTTCT+TGG 0.369111 7.2:+30758645 MS.gene27546:CDS
GTTTCTGTTGCTGGTTCTGT+TGG 0.383646 7.2:-30756434 None:intergenic
AAACCTCAGCTGCAACAAAC+TGG 0.395103 7.2:+30756527 MS.gene27546:CDS
TATGTGAGACTCACTGGATT+AGG 0.397735 7.2:+30757522 MS.gene27546:CDS
ATCTTCACTACTAATGTTGC+TGG 0.414538 7.2:-30755864 None:intergenic
TAACTCTTCACCTTCTATGA+TGG 0.414564 7.2:+30756501 MS.gene27546:CDS
TGAGGAATTGTTGAGATTGT+TGG 0.418805 7.2:-30756305 None:intergenic
CAGAAGCTGTTGCTGTTCAT+TGG 0.419326 7.2:+30757682 MS.gene27546:CDS
TTCATTCTTGGGAAGGTTTG+TGG 0.421301 7.2:+30758398 MS.gene27546:CDS
TCAAGAACAAGAGCAAGGTT+TGG 0.421529 7.2:-30756646 None:intergenic
CAAAAGCTTCTTGTATAGCT+TGG 0.424689 7.2:-30757398 None:intergenic
ATGAGTGTAGTTTGAGGTTG+AGG 0.424711 7.2:-30756323 None:intergenic
GCTGCAACAAACTGGATTGA+TGG 0.429027 7.2:+30756535 MS.gene27546:CDS
ATGGTATCCTTAAACTTGGT+TGG 0.429657 7.2:+30758706 MS.gene27546:CDS
AGCAATGCAGGTTCATTCTT+GGG 0.429990 7.2:+30758387 MS.gene27546:CDS
CCCTTCTGAAGGGTATACTT+TGG 0.433004 7.2:+30758674 MS.gene27546:CDS
AGACTAGTGAGTTCATGTTT+AGG 0.434001 7.2:+30757264 MS.gene27546:CDS
GATTCTCTTGGTTCAAGCTA+TGG 0.434363 7.2:+30758450 MS.gene27546:CDS
TGTTGCTGGTTCTGTTGGGT+TGG 0.440162 7.2:-30756429 None:intergenic
TTTCCTGTTGCTGAGAAAGT+TGG 0.444010 7.2:+30757624 MS.gene27546:CDS
CATATCCTTGCTTCAAGACC+AGG 0.445706 7.2:+30757489 MS.gene27546:CDS
TTTCTGTTGCTGGTTCTGTT+GGG 0.451663 7.2:-30756433 None:intergenic
CTTGGCATGTTCCCTTCTGA+AGG 0.451744 7.2:+30758663 MS.gene27546:CDS
GTTTGCTTACTGCTTCTGCT+TGG 0.455432 7.2:+30758739 MS.gene27546:CDS
TTGCTCTTGTTCTTGAACAT+AGG 0.455705 7.2:+30756653 MS.gene27546:CDS
TTGAGATTGTTGGAAGAAGA+AGG 0.456597 7.2:-30756295 None:intergenic
CGTAACGTTCTTGCAGTTGG+TGG 0.465242 7.2:+30758528 MS.gene27546:CDS
TTTAGAGGTATCTCTTTGGC+TGG 0.469891 7.2:+30758612 MS.gene27546:CDS
GTTTGAAGCACTATTGTTGT+TGG 0.471893 7.2:-30755840 None:intergenic
CCTCCTTATGTGAGACTCAC+TGG 0.475283 7.2:+30757516 MS.gene27546:CDS
GCAACAGGAAAGAACTCAAA+AGG 0.485861 7.2:-30757612 None:intergenic
TTGTTGTTGTGGCATGTTGG+TGG 0.486113 7.2:-30755909 None:intergenic
GAACAAGACTTGAGCAATGC+AGG 0.491335 7.2:+30758375 MS.gene27546:CDS
TTTCTCTCAGGAACAACACT+TGG 0.491378 7.2:-30756700 None:intergenic
GTTCAGATACAAACACTTTG+TGG 0.494320 7.2:+30757733 MS.gene27546:CDS
TTGAAAATGAGTGTAGTTTG+AGG 0.504163 7.2:-30756329 None:intergenic
AAATGAGAGAATTTCACAAA+TGG 0.504284 7.2:-30757366 None:intergenic
ATGTAGGTTGGCACCTAAAG+TGG 0.505838 7.2:+30758341 MS.gene27546:intron
TGAAGGGTATACTTTGGTTG+AGG 0.506995 7.2:+30758680 MS.gene27546:CDS
TAGCAACATAGTGGAGTAGT+TGG 0.507219 7.2:-30756272 None:intergenic
TATGACATTGTTGGAATGAA+AGG 0.510105 7.2:-30758769 None:intergenic
AATCCAGTTTGTTGCAGCTG+AGG 0.510974 7.2:-30756530 None:intergenic
GAATGAGGTTGTTGTTGTTG+TGG 0.514198 7.2:-30755920 None:intergenic
AAAATCTATTGTTGTTGTTG+TGG 0.516540 7.2:-30756000 None:intergenic
CACTGGATTAGGTACTTCCA+TGG 0.517366 7.2:+30757533 MS.gene27546:CDS
ATGGAAACACTTGAAGCTAC+TGG 0.517893 7.2:+30757552 MS.gene27546:CDS
CTCACATAAGGAGGTCCACC+TGG 0.520829 7.2:-30757507 None:intergenic
TTGTTGTTGTTGTGGCATGT+TGG 0.522020 7.2:-30755912 None:intergenic
AGTAGCTTCAAGTGTTTCCA+TGG 0.526271 7.2:-30757550 None:intergenic
CAAAACATGTTTCCTAATTG+GGG 0.527048 7.2:+30756865 MS.gene27546:CDS
ATGCAAGGTTTACAATGGCC+TGG 0.527385 7.2:+30757459 MS.gene27546:CDS
GATAATGGTATCCTTAAACT+TGG 0.528102 7.2:+30758702 MS.gene27546:CDS
ATTCGTAACGTTCTTGCAGT+TGG 0.528879 7.2:+30758525 MS.gene27546:CDS
ACACTTTGTGGCTCTTGCAA+AGG 0.531826 7.2:+30757745 MS.gene27546:CDS
TTGGCATGTTCCCTTCTGAA+GGG 0.532685 7.2:+30758664 MS.gene27546:CDS
AGTAACTGTAACATAAGTTC+TGG 0.539385 7.2:+30756457 MS.gene27546:CDS
CTAACATTGTTGATGAGTTG+AGG 0.539907 7.2:-30756601 None:intergenic
AGAGCAAGGTTTGGATTACA+AGG 0.541984 7.2:-30756637 None:intergenic
ACCAAAGTATACCCTTCAGA+AGG 0.543263 7.2:-30758675 None:intergenic
TTGGTTGTGACGGTCACAGT+GGG 0.545379 7.2:-30756253 None:intergenic
TTGCAGTTGGTGGACCATCA+AGG 0.546702 7.2:+30758538 MS.gene27546:CDS
ATGCAGGTTCATTCTTGGGA+AGG 0.550894 7.2:+30758391 MS.gene27546:CDS
GAGGTTTCATCCATCATAGA+AGG 0.554125 7.2:-30756511 None:intergenic
TCATACCACATCATAACTGA+TGG 0.555692 7.2:+30758787 MS.gene27546:CDS
GAGGAATTAACATTAGAAGC+AGG 0.557000 7.2:-30756769 None:intergenic
AGAAGAAGGTAGCAACATAG+TGG 0.560888 7.2:-30756281 None:intergenic
ATTGTTGATGAGTTGAGGAA+TGG 0.561897 7.2:-30756596 None:intergenic
ATGATGTGGTATGACATTGT+TGG 0.567016 7.2:-30758778 None:intergenic
AATCCAGTGAGTCTCACATA+AGG 0.568715 7.2:-30757519 None:intergenic
ATTATTATTATTTGTACCTG+CGG 0.572319 7.2:-30756116 None:intergenic
ATAGATCTTGATATCATGCA+AGG 0.576982 7.2:+30757444 MS.gene27546:CDS
AACCATGTTCCTAGTATCTG+TGG 0.578504 7.2:+30756373 MS.gene27546:CDS
AGAGAGTGAAGAGAGACATG+TGG 0.579883 7.2:+30758476 MS.gene27546:CDS
GTCCACCTGGTCTTGAAGCA+AGG 0.584919 7.2:-30757494 None:intergenic
TGATTATGTAGTGTGTGTGG+TGG 0.599368 7.2:-30757303 None:intergenic
ATATCATGCAAGGTTTACAA+TGG 0.601849 7.2:+30757454 MS.gene27546:CDS
ATGACATTGTTGGAATGAAA+GGG 0.601926 7.2:-30758768 None:intergenic
TATGTTCAAGAACAAGAGCA+AGG 0.620046 7.2:-30756651 None:intergenic
GAGTGAAGAGAGACATGTGG+TGG 0.624909 7.2:+30758479 MS.gene27546:CDS
CCAAAGTATACCCTTCAGAA+GGG 0.626039 7.2:-30758674 None:intergenic
AGTGGAGTAGTTGGTTGTGA+CGG 0.635058 7.2:-30756263 None:intergenic
TAGATCCATCAGTTATGATG+TGG 0.639240 7.2:-30758792 None:intergenic
ACCTAAAGTGGTAACAGTAG+TGG 0.640826 7.2:+30758353 MS.gene27546:CDS
CCAGTGAGTCTCACATAAGG+AGG 0.642698 7.2:-30757516 None:intergenic
AACCACAGATACTAGGAACA+TGG 0.643167 7.2:-30756375 None:intergenic
CTTTGTGCTGATGTACCGAA+AGG 0.646157 7.2:-30757207 None:intergenic
ACAACAACAACACTAGCCGC+AGG 0.647867 7.2:+30756100 MS.gene27546:CDS
GTTGGTTGTGACGGTCACAG+TGG 0.651008 7.2:-30756254 None:intergenic
ATCCTTGCTTCAAGACCAGG+TGG 0.657302 7.2:+30757492 MS.gene27546:CDS
AGAGAAAAGCTTCAACAGTG+TGG 0.663978 7.2:+30758588 MS.gene27546:CDS
ACATTGTTGGAATGAAAGGG+AGG 0.683514 7.2:-30758765 None:intergenic
AGGTAAAGAACAATTCAATG+AGG 0.690919 7.2:-30756053 None:intergenic
GTTGGTGGACCATCAAGGAG+TGG 0.725373 7.2:+30758543 MS.gene27546:CDS
GAGGAACAATGTCATCAACA+CGG 0.736939 7.2:-30756807 None:intergenic
TGGTTGTGACGGTCACAGTG+GGG 0.753890 7.2:-30756252 None:intergenic
ACGGTTCATGAGTTTCACTG+AGG 0.765117 7.2:-30756788 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAAAAAAGTATAAAGTTT+TGG - chr7.2:30757933-30757952 None:intergenic 10.0%
!!! AAAAAAAAGTATAAAGTTTT+GGG - chr7.2:30757932-30757951 None:intergenic 10.0%
!! ATATAATCTACATGATTTAG+TGG + chr7.2:30757866-30757885 MS.gene27546:intron 20.0%
!! ATTATTATTATTTGTACCTG+CGG - chr7.2:30756119-30756138 None:intergenic 20.0%
!! GGTAATAGTAATAAAAGTTT+AGG + chr7.2:30757770-30757789 MS.gene27546:intron 20.0%
!! TATAGTTTCTAATTATCCAT+TGG + chr7.2:30758161-30758180 MS.gene27546:intron 20.0%
!! TTCAAAAATACACAAATAAG+AGG + chr7.2:30758262-30758281 MS.gene27546:intron 20.0%
!! TTTATCTGTGTTATATATGT+AGG + chr7.2:30758325-30758344 MS.gene27546:intron 20.0%
!!! TTATTGTTGTTATTGATTTG+TGG - chr7.2:30756895-30756914 None:intergenic 20.0%
! AAATGAGAGAATTTCACAAA+TGG - chr7.2:30757369-30757388 None:intergenic 25.0%
! AATAACAAGAAAGAAGAAAG+AGG + chr7.2:30757047-30757066 MS.gene27546:intron 25.0%
! ATCAAAACATGTTTCCTAAT+TGG + chr7.2:30756863-30756882 MS.gene27546:CDS 25.0%
! ATTGAAGAAGTAATGTTAGA+AGG - chr7.2:30757113-30757132 None:intergenic 25.0%
! CAAACTATAACAACATTTCA+TGG - chr7.2:30757808-30757827 None:intergenic 25.0%
! GTGAGATAAAGTTTCATAAT+TGG + chr7.2:30758565-30758584 MS.gene27546:CDS 25.0%
! TATACAAAACCAACAAGAAT+TGG - chr7.2:30757847-30757866 None:intergenic 25.0%
! TCAAAACATGTTTCCTAATT+GGG + chr7.2:30756864-30756883 MS.gene27546:CDS 25.0%
!! AAAATCTATTGTTGTTGTTG+TGG - chr7.2:30756003-30756022 None:intergenic 25.0%
!! TTACCTTTAGAAGAGAAAAA+AGG - chr7.2:30757990-30758009 None:intergenic 25.0%
!! TTCTCTTCTAAAGGTAAAAT+TGG + chr7.2:30757993-30758012 MS.gene27546:intron 25.0%
!!! AAAAGTGCTTAGAAAAAGAA+TGG + chr7.2:30755949-30755968 MS.gene27546:CDS 25.0%
AATTATCCATTGGATTTGCA+TGG + chr7.2:30758171-30758190 MS.gene27546:intron 30.0%
AGGTAAAGAACAATTCAATG+AGG - chr7.2:30756056-30756075 None:intergenic 30.0%
AGTAACTGTAACATAAGTTC+TGG + chr7.2:30756457-30756476 MS.gene27546:CDS 30.0%
ATAGATCTTGATATCATGCA+AGG + chr7.2:30757444-30757463 MS.gene27546:CDS 30.0%
ATATCATGCAAGGTTTACAA+TGG + chr7.2:30757454-30757473 MS.gene27546:CDS 30.0%
ATGACATTGTTGGAATGAAA+GGG - chr7.2:30758771-30758790 None:intergenic 30.0%
ATTATCCATTGGATTTGCAT+GGG + chr7.2:30758172-30758191 MS.gene27546:intron 30.0%
CAAAACATGTTTCCTAATTG+GGG + chr7.2:30756865-30756884 MS.gene27546:CDS 30.0%
CAAAAGAAGAAAGATGAAGA+AGG + chr7.2:30757081-30757100 MS.gene27546:intron 30.0%
CAATAACAACAATAACCCTT+TGG + chr7.2:30756900-30756919 MS.gene27546:intron 30.0%
CATTCTCTATATGATGTTAC+TGG + chr7.2:30757711-30757730 MS.gene27546:CDS 30.0%
CTTGATGAATCAGAAAAATG+AGG - chr7.2:30756829-30756848 None:intergenic 30.0%
TATACTTTGGTTGAGGATAA+TGG + chr7.2:30758687-30758706 MS.gene27546:CDS 30.0%
TATGACATTGTTGGAATGAA+AGG - chr7.2:30758772-30758791 None:intergenic 30.0%
TCAGCATTATTTGATTCTCT+TGG + chr7.2:30758438-30758457 MS.gene27546:CDS 30.0%
TCTGTGTTATATATGTAGGT+TGG + chr7.2:30758329-30758348 MS.gene27546:intron 30.0%
TTGAAAATGAGTGTAGTTTG+AGG - chr7.2:30756332-30756351 None:intergenic 30.0%
! AAAAAGAATGGCTTCTGAAA+TGG + chr7.2:30755961-30755980 MS.gene27546:CDS 30.0%
! GATAATGGTATCCTTAAACT+TGG + chr7.2:30758702-30758721 MS.gene27546:CDS 30.0%
! TACTCTTTTCCAATTCTTGT+TGG + chr7.2:30757835-30757854 MS.gene27546:intron 30.0%
! TCTGATTTTGCATCTAAACT+TGG + chr7.2:30757585-30757604 MS.gene27546:CDS 30.0%
! TTTGCATGGGATTTTTATTC+TGG + chr7.2:30758185-30758204 MS.gene27546:intron 30.0%
!! TTTTGATTATGTAGTGTGTG+TGG - chr7.2:30757309-30757328 None:intergenic 30.0%
!!! CTACCTTTTTTCTCTTCTAA+AGG + chr7.2:30757984-30758003 MS.gene27546:intron 30.0%
AAAATCCCATGCAAATCCAA+TGG - chr7.2:30758180-30758199 None:intergenic 35.0%
AGACTAGTGAGTTCATGTTT+AGG + chr7.2:30757264-30757283 MS.gene27546:CDS 35.0%
ATCTTCACTACTAATGTTGC+TGG - chr7.2:30755867-30755886 None:intergenic 35.0%
ATGATGTGGTATGACATTGT+TGG - chr7.2:30758781-30758800 None:intergenic 35.0%
ATGGTATCCTTAAACTTGGT+TGG + chr7.2:30758706-30758725 MS.gene27546:CDS 35.0%
CAAAAGCTTCTTGTATAGCT+TGG - chr7.2:30757401-30757420 None:intergenic 35.0%
GAGGAATTAACATTAGAAGC+AGG - chr7.2:30756772-30756791 None:intergenic 35.0%
GATCTTTCCAACCAAGTTTA+AGG - chr7.2:30758716-30758735 None:intergenic 35.0%
GTGAAGGTAAAGTAACCAAA+GGG - chr7.2:30756918-30756937 None:intergenic 35.0%
GTGAGCACTATAGTCTATAA+AGG - chr7.2:30757957-30757976 None:intergenic 35.0%
GTTCAGATACAAACACTTTG+TGG + chr7.2:30757733-30757752 MS.gene27546:CDS 35.0%
TAACTCTTCACCTTCTATGA+TGG + chr7.2:30756501-30756520 MS.gene27546:CDS 35.0%
TATGTTCAAGAACAAGAGCA+AGG - chr7.2:30756654-30756673 None:intergenic 35.0%
TGAGGAATTGTTGAGATTGT+TGG - chr7.2:30756308-30756327 None:intergenic 35.0%
TGATTTAGTGGTTGCTGATA+TGG + chr7.2:30757878-30757897 MS.gene27546:intron 35.0%
TGTGAAGGTAAAGTAACCAA+AGG - chr7.2:30756919-30756938 None:intergenic 35.0%
TTACCTTCATCCACAGAAAA+AGG + chr7.2:30756070-30756089 MS.gene27546:CDS 35.0%
TTGAGATTGTTGGAAGAAGA+AGG - chr7.2:30756298-30756317 None:intergenic 35.0%
TTGCTCTTGTTCTTGAACAT+AGG + chr7.2:30756653-30756672 MS.gene27546:CDS 35.0%
! AGCACTTTTCTTTCAGAATG+AGG - chr7.2:30755938-30755957 None:intergenic 35.0%
! ATTGTTGATGAGTTGAGGAA+TGG - chr7.2:30756599-30756618 None:intergenic 35.0%
! CTAACATTGTTGATGAGTTG+AGG - chr7.2:30756604-30756623 None:intergenic 35.0%
! GTTTGAAGCACTATTGTTGT+TGG - chr7.2:30755843-30755862 None:intergenic 35.0%
! TGTTGTAACACCTTTTTCTG+TGG - chr7.2:30756083-30756102 None:intergenic 35.0%
!! CTGGATTGATGGTATTTTGA+AGG + chr7.2:30756546-30756565 MS.gene27546:CDS 35.0%
!! TGATCTTGTTGTGTTGATTG+TGG - chr7.2:30756943-30756962 None:intergenic 35.0%
!! TTTTCAACAACCACTTGTTG+AGG + chr7.2:30756345-30756364 MS.gene27546:CDS 35.0%
!!! GTGTTGTTTCTTTTTCTCTC+AGG - chr7.2:30756715-30756734 None:intergenic 35.0%
!!! TCAGCTTTTCAAGTCTTCAA+TGG + chr7.2:30757336-30757355 MS.gene27546:CDS 35.0%
!!! TGGTTTTAGAGGTATCTCTT+TGG + chr7.2:30758608-30758627 MS.gene27546:CDS 35.0%
!!! TGGTTTTGAGATGGAAAAAG+TGG - chr7.2:30756412-30756431 None:intergenic 35.0%
AACCACAGATACTAGGAACA+TGG - chr7.2:30756378-30756397 None:intergenic 40.0%
AACCATGTTCCTAGTATCTG+TGG + chr7.2:30756373-30756392 MS.gene27546:CDS 40.0%
AATCCAGTGAGTCTCACATA+AGG - chr7.2:30757522-30757541 None:intergenic 40.0%
ACATTGTTGGAATGAAAGGG+AGG - chr7.2:30758768-30758787 None:intergenic 40.0%
ACCAAAGTATACCCTTCAGA+AGG - chr7.2:30758678-30758697 None:intergenic 40.0%
ACCTAAAGTGGTAACAGTAG+TGG + chr7.2:30758353-30758372 MS.gene27546:CDS 40.0%
AGAAGAAGGTAGCAACATAG+TGG - chr7.2:30756284-30756303 None:intergenic 40.0%
AGAGAAAAGCTTCAACAGTG+TGG + chr7.2:30758588-30758607 MS.gene27546:CDS 40.0%
AGAGCAAGGTTTGGATTACA+AGG - chr7.2:30756640-30756659 None:intergenic 40.0%
AGCAATGCAGGTTCATTCTT+GGG + chr7.2:30758387-30758406 MS.gene27546:CDS 40.0%
AGTAGCTTCAAGTGTTTCCA+TGG - chr7.2:30757553-30757572 None:intergenic 40.0%
ATGGAAACACTTGAAGCTAC+TGG + chr7.2:30757552-30757571 MS.gene27546:CDS 40.0%
ATTCGTAACGTTCTTGCAGT+TGG + chr7.2:30758525-30758544 MS.gene27546:CDS 40.0%
CCAAAGTATACCCTTCAGAA+GGG - chr7.2:30758677-30758696 None:intergenic 40.0%
CCAGAAAAACCACAGATACT+AGG - chr7.2:30756385-30756404 None:intergenic 40.0%
GAGGAACAATGTCATCAACA+CGG - chr7.2:30756810-30756829 None:intergenic 40.0%
GAGGTTTCATCCATCATAGA+AGG - chr7.2:30756514-30756533 None:intergenic 40.0%
GATTCTCTTGGTTCAAGCTA+TGG + chr7.2:30758450-30758469 MS.gene27546:CDS 40.0%
GCAACAGGAAAGAACTCAAA+AGG - chr7.2:30757615-30757634 None:intergenic 40.0%
GCAAGGATATGAAAAAGACC+AGG - chr7.2:30757480-30757499 None:intergenic 40.0%
TATGTGAGACTCACTGGATT+AGG + chr7.2:30757522-30757541 MS.gene27546:CDS 40.0%
TCAAGAACAAGAGCAAGGTT+TGG - chr7.2:30756649-30756668 None:intergenic 40.0%
TCCACTACTGTTACCACTTT+AGG - chr7.2:30758357-30758376 None:intergenic 40.0%
TGATTATGTAGTGTGTGTGG+TGG - chr7.2:30757306-30757325 None:intergenic 40.0%
TTCATTCTTGGGAAGGTTTG+TGG + chr7.2:30758398-30758417 MS.gene27546:CDS 40.0%
TTTATCTCACCACTCCTTGA+TGG - chr7.2:30758555-30758574 None:intergenic 40.0%
TTTCCAACTTTCTCAGCAAC+AGG - chr7.2:30757630-30757649 None:intergenic 40.0%
TTTCCTGTTGCTGAGAAAGT+TGG + chr7.2:30757624-30757643 MS.gene27546:CDS 40.0%
TTTCTCTCAGGAACAACACT+TGG - chr7.2:30756703-30756722 None:intergenic 40.0%
! ACACCTTTTTCTGTGGATGA+AGG - chr7.2:30756076-30756095 None:intergenic 40.0%
! ATGAGTGTAGTTTGAGGTTG+AGG - chr7.2:30756326-30756345 None:intergenic 40.0%
! CCTAGTATCTGTGGTTTTTC+TGG + chr7.2:30756382-30756401 MS.gene27546:CDS 40.0%
! GAATGAGGTTGTTGTTGTTG+TGG - chr7.2:30755923-30755942 None:intergenic 40.0%
! TAGCAACATAGTGGAGTAGT+TGG - chr7.2:30756275-30756294 None:intergenic 40.0%
! TGAAGGGTATACTTTGGTTG+AGG + chr7.2:30758680-30758699 MS.gene27546:CDS 40.0%
! TTGTTGTTGTTGTGGCATGT+TGG - chr7.2:30755915-30755934 None:intergenic 40.0%
!! ACACAAGCTAGTCTTCTTCT+TGG + chr7.2:30758645-30758664 MS.gene27546:CDS 40.0%
!! ACAGTTACTGTTTCTGTTGC+TGG - chr7.2:30756446-30756465 None:intergenic 40.0%
!! TGTGTTGATTGTGGTTGTGA+AGG - chr7.2:30756934-30756953 None:intergenic 40.0%
!! TTTAGAGGTATCTCTTTGGC+TGG + chr7.2:30758612-30758631 MS.gene27546:CDS 40.0%
!! TTTCTGTTGCTGGTTCTGTT+GGG - chr7.2:30756436-30756455 None:intergenic 40.0%
!!! CTTCAACAGTGTGGTTTTAG+AGG + chr7.2:30758597-30758616 MS.gene27546:CDS 40.0%
!!! TGGTTTTGTTCCTCAACAAG+TGG - chr7.2:30756358-30756377 None:intergenic 40.0%
AAACCTCAGCTGCAACAAAC+TGG + chr7.2:30756527-30756546 MS.gene27546:CDS 45.0%
ACACTTTGTGGCTCTTGCAA+AGG + chr7.2:30757745-30757764 MS.gene27546:CDS 45.0%
ACGGTTCATGAGTTTCACTG+AGG - chr7.2:30756791-30756810 None:intergenic 45.0%
AGAGAGTGAAGAGAGACATG+TGG + chr7.2:30758476-30758495 MS.gene27546:CDS 45.0%
AGTGGAGTAGTTGGTTGTGA+CGG - chr7.2:30756266-30756285 None:intergenic 45.0%
ATGCAAGGTTTACAATGGCC+TGG + chr7.2:30757459-30757478 MS.gene27546:CDS 45.0%
ATGCAGGTTCATTCTTGGGA+AGG + chr7.2:30758391-30758410 MS.gene27546:CDS 45.0%
ATTGCCAACTCAGCAGAAGT+TGG - chr7.2:30757030-30757049 None:intergenic 45.0%
ATTTGTGGCACACCCCAATT+AGG - chr7.2:30756880-30756899 None:intergenic 45.0%
CATATCCTTGCTTCAAGACC+AGG + chr7.2:30757489-30757508 MS.gene27546:CDS 45.0%
GAACAAGACTTGAGCAATGC+AGG + chr7.2:30758375-30758394 MS.gene27546:CDS 45.0%
GAGCAATGCAGGTTCATTCT+TGG + chr7.2:30758386-30758405 MS.gene27546:CDS 45.0%
GCTGCAACAAACTGGATTGA+TGG + chr7.2:30756535-30756554 MS.gene27546:CDS 45.0%
GTTTGCTTACTGCTTCTGCT+TGG + chr7.2:30758739-30758758 MS.gene27546:CDS 45.0%
TTGGCATGTTCCCTTCTGAA+GGG + chr7.2:30758664-30758683 MS.gene27546:CDS 45.0%
TTTGTGGCTCTTGCAAAGGT+TGG + chr7.2:30757749-30757768 MS.gene27546:intron 45.0%
! ATGTAGGTTGGCACCTAAAG+TGG + chr7.2:30758341-30758360 MS.gene27546:intron 45.0%
! CACTGGATTAGGTACTTCCA+TGG + chr7.2:30757533-30757552 MS.gene27546:CDS 45.0%
! CAGAAGCTGTTGCTGTTCAT+TGG + chr7.2:30757682-30757701 MS.gene27546:CDS 45.0%
! CCCTTCTGAAGGGTATACTT+TGG + chr7.2:30758674-30758693 MS.gene27546:CDS 45.0%
! CTTTGTGCTGATGTACCGAA+AGG - chr7.2:30757210-30757229 None:intergenic 45.0%
! GCTGCTTGTGCTTTGTTCAA+TGG + chr7.2:30755800-30755819 MS.gene27546:CDS 45.0%
! TTGTTGTTGTGGCATGTTGG+TGG - chr7.2:30755912-30755931 None:intergenic 45.0%
!! AATCCAGTTTGTTGCAGCTG+AGG - chr7.2:30756533-30756552 None:intergenic 45.0%
!! GTTTCTGTTGCTGGTTCTGT+TGG - chr7.2:30756437-30756456 None:intergenic 45.0%
!!! CTGTTGGGTTGGTTTTGAGA+TGG - chr7.2:30756421-30756440 None:intergenic 45.0%
ACAACAACAACACTAGCCGC+AGG + chr7.2:30756100-30756119 MS.gene27546:CDS 50.0%
ACCACCAACTTCTGCTGAGT+TGG + chr7.2:30757023-30757042 MS.gene27546:intron 50.0%
ATCCTTGCTTCAAGACCAGG+TGG + chr7.2:30757492-30757511 MS.gene27546:CDS 50.0%
CCAGTGAGTCTCACATAAGG+AGG - chr7.2:30757519-30757538 None:intergenic 50.0%
CCTCCTTATGTGAGACTCAC+TGG + chr7.2:30757516-30757535 MS.gene27546:CDS 50.0%
CGTAACGTTCTTGCAGTTGG+TGG + chr7.2:30758528-30758547 MS.gene27546:CDS 50.0%
CTTGGCATGTTCCCTTCTGA+AGG + chr7.2:30758663-30758682 MS.gene27546:CDS 50.0%
GAGTGAAGAGAGACATGTGG+TGG + chr7.2:30758479-30758498 MS.gene27546:CDS 50.0%
TCGCAGCTTTCAACGCCTTT+CGG + chr7.2:30757192-30757211 MS.gene27546:CDS 50.0%
TTGGTTGTGACGGTCACAGT+GGG - chr7.2:30756256-30756275 None:intergenic 50.0%
!! AACTCAGCAGAAGTTGGTGG+TGG - chr7.2:30757024-30757043 None:intergenic 50.0%
!! TGTTGCTGGTTCTGTTGGGT+TGG - chr7.2:30756432-30756451 None:intergenic 50.0%
!! TTGCAGTTGGTGGACCATCA+AGG + chr7.2:30758538-30758557 MS.gene27546:CDS 50.0%
CTCACATAAGGAGGTCCACC+TGG - chr7.2:30757510-30757529 None:intergenic 55.0%
GCCAACTCAGCAGAAGTTGG+TGG - chr7.2:30757027-30757046 None:intergenic 55.0%
GTTGGTTGTGACGGTCACAG+TGG - chr7.2:30756257-30756276 None:intergenic 55.0%
TGGTTGTGACGGTCACAGTG+GGG - chr7.2:30756255-30756274 None:intergenic 55.0%
! GTTGGTGGACCATCAAGGAG+TGG + chr7.2:30758543-30758562 MS.gene27546:CDS 55.0%
!! GTCCACCTGGTCTTGAAGCA+AGG - chr7.2:30757497-30757516 None:intergenic 55.0%
!! TCAGCAGAAGTTGGTGGTGG+TGG - chr7.2:30757021-30757040 None:intergenic 55.0%
!! GGTGGTGGTGGTGTTGTTGC+AGG - chr7.2:30757009-30757028 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 30755794 30758806 30755794 ID=MS.gene27546
chr7.2 mRNA 30755794 30758806 30755794 ID=MS.gene27546.t1;Parent=MS.gene27546
chr7.2 exon 30755794 30756886 30755794 ID=MS.gene27546.t1.exon1;Parent=MS.gene27546.t1
chr7.2 CDS 30755794 30756886 30755794 ID=cds.MS.gene27546.t1;Parent=MS.gene27546.t1
chr7.2 exon 30757139 30757766 30757139 ID=MS.gene27546.t1.exon2;Parent=MS.gene27546.t1
chr7.2 CDS 30757139 30757766 30757139 ID=cds.MS.gene27546.t1;Parent=MS.gene27546.t1
chr7.2 exon 30758347 30758806 30758347 ID=MS.gene27546.t1.exon3;Parent=MS.gene27546.t1
chr7.2 CDS 30758347 30758806 30758347 ID=cds.MS.gene27546.t1;Parent=MS.gene27546.t1
Gene Sequence

>MS.gene27546

ATGGCTGCTGCTTGTGCTTTGTTCAATGGTGCTACTACTGATGAAACCAACAACAATAGTGCTTCAAACTCCAGCAACATTAGTAGTGAAGATTTACACACTCTACACAACAACACCACCAACATGCCACAACAACAACAACCTCATTCTGAAAGAAAAGTGCTTAGAAAAAGAATGGCTTCTGAAATGGAACTTCAACTTCAAGTCCACAACAACAACAATAGATTTTCTCGTAGAAACAACAACAACAACAACATATCCTCATTGAATTGTTCTTTACCTTCATCCACAGAAAAAGGTGTTACAACAACAACAACACTAGCCGCAGGTACAAATAATAATAATAATATTAATAATAATAACTTTCATTATAATACTAATAATATAAACAATAGTAATAATATTAGTCATCATAGTAACGTTGCTTTGTCACGAGATAACGTTGCTGTTCAAAATTACCCCACTGTGACCGTCACAACCAACTACTCCACTATGTTGCTACCTTCTTCTTCCAACAATCTCAACAATTCCTCAACCTCAAACTACACTCATTTTCAACAACCACTTGTTGAGGAACAAAACCATGTTCCTAGTATCTGTGGTTTTTCTGGTTTACCACTTTTTCCATCTCAAAACCAACCCAACAGAACCAGCAACAGAAACAGTAACTGTAACATAAGTTCTGGTACTAATATTGTTGATGTTGTTAACTCTTCACCTTCTATGATGGATGAAACCTCAGCTGCAACAAACTGGATTGATGGTATTTTGAAGGATCTTATTCATACTTCAAACAGTGTTTCCATTCCTCAACTCATCAACAATGTTAGAGAGATTATATATCCTTGTAATCCAAACCTTGCTCTTGTTCTTGAACATAGGTTACGTCTTCTTACAGAAACTGCTCCAAGTGTTGTTCCTGAGAGAAAAAGAAACAACACTGAACAACAAAGTGTTGCTAATGTTAATGTTTTACCTGCTTCTAATGTTAATTCCTCAGTGAAACTCATGAACCGTGTTGATGACATTGTTCCTCATTTTTCTGATTCATCAAGTCTTTTGAATCAAAATCAAAACATGTTTCCTAATTGGGGTGTGCCACAAATCAATAACAACAATAACCCTTTGGTTACTTTACCTTCACAACCACAATCAACACAACAAGATCAACATCAACAACATCAACATCAAGAGCATCAAGAAGATCTTGCACCTGCAACAACACCACCACCACCAACTTCTGCTGAGTTGGCAATAACAAGAAAGAAGAAAGAGGAGATTAAAGAACAAAAGAAGAAAGATGAAGAAGGTTTACACCTTCTAACATTACTTCTTCAATGTGCAGAAGCAGTATCAGCAGAGAATCTTGAACAAGCAAACAAGATGTTACTCGAAATTTCGCAGCTTTCAACGCCTTTCGGTACATCAGCACAAAGAGTAGCAGCTTATTTCTCAGAAGCAATTTCAGCAAGACTAGTGAGTTCATGTTTAGGAATCTATGCAACACTTCCACCACACACACTACATAATCAAAAAGTTGCTTCAGCTTTTCAAGTCTTCAATGGTATTAGTCCATTTGTGAAATTCTCTCATTTCACTGCTAACCAAGCTATACAAGAAGCTTTTGATAGAGAAGAAAGAGTTCATATCATAGATCTTGATATCATGCAAGGTTTACAATGGCCTGGTCTTTTTCATATCCTTGCTTCAAGACCAGGTGGACCTCCTTATGTGAGACTCACTGGATTAGGTACTTCCATGGAAACACTTGAAGCTACTGGTAAACGTTTGTCTGATTTTGCATCTAAACTTGGTCTTCCTTTTGAGTTCTTTCCTGTTGCTGAGAAAGTTGGAAACATTGATGTTGAGAAGCTTAATGTTAGTAAATCAGAAGCTGTTGCTGTTCATTGGTTGCAACATTCTCTATATGATGTTACTGGTTCAGATACAAACACTTTGTGGCTCTTGCAAAGGTTGGTAATAGTAATAAAAGTTTAGGTGCATATACTCTCCATGAAATGTTGTTATAGTTTGTTATTGTTACTCTTTTCCAATTCTTGTTGGTTTTGTATATATAATCTACATGATTTAGTGGTTGCTGATATGGAAAAATAGCACTTTTTCATGAAAGATTTCCCAAAACTTTATACTTTTTTTTTACCTTTATAGACTATAGTGCTCACTTTATGTCTACCTTTTTTCTCTTCTAAAGGTAAAATTGGAAGCTATGATTATGTCTTTGTATAAATTAATAGATAGAAACAAAAAGAACTATATGTTATTATGATATGTTTATAGTAAGTTTTGAAAGACATAGCTCTCATGTAAGTTGATTATTACAATTTACAGACATGACATTAATATGTCTATAGTTTCTAATTATCCATTGGATTTGCATGGGATTTTTATTCTGGTTATGTGTTTATCTCTGTCTCTCTTACTTTTTATCTTTTACGAATTACGCACATTTCAAAAATACACAAATAAGAGGACATCGTATTTGAAATCAAAATTTTGATGTCAAAAACTCATTTATCTGTGTTATATATGTAGGTTGGCACCTAAAGTGGTAACAGTAGTGGAACAAGACTTGAGCAATGCAGGTTCATTCTTGGGAAGGTTTGTGGAAGCAATACATTACTACTCAGCATTATTTGATTCTCTTGGTTCAAGCTATGGAGAAGAGAGTGAAGAGAGACATGTGGTGGAGCAGCAACTATTATCAAGAGAGATTCGTAACGTTCTTGCAGTTGGTGGACCATCAAGGAGTGGTGAGATAAAGTTTCATAATTGGAGAGAAAAGCTTCAACAGTGTGGTTTTAGAGGTATCTCTTTGGCTGGTAATGCTGCTACACAAGCTAGTCTTCTTCTTGGCATGTTCCCTTCTGAAGGGTATACTTTGGTTGAGGATAATGGTATCCTTAAACTTGGTTGGAAAGATCTTTGTTTGCTTACTGCTTCTGCTTGGAGACCTCCCTTTCATTCCAACAATGTCATACCACATCATAACTGA
Protein sequence

>MS.gene27546.t1

MAAACALFNGATTDETNNNSASNSSNISSEDLHTLHNNTTNMPQQQQPHSERKVLRKRMASEMELQLQVHNNNNRFSRRNNNNNNISSLNCSLPSSTEKGVTTTTTLAAGTNNNNNINNNNFHYNTNNINNSNNISHHSNVALSRDNVAVQNYPTVTVTTNYSTMLLPSSSNNLNNSSTSNYTHFQQPLVEEQNHVPSICGFSGLPLFPSQNQPNRTSNRNSNCNISSGTNIVDVVNSSPSMMDETSAATNWIDGILKDLIHTSNSVSIPQLINNVREIIYPCNPNLALVLEHRLRLLTETAPSVVPERKRNNTEQQSVANVNVLPASNVNSSVKLMNRVDDIVPHFSDSSSLLNQNQNMFPNWEAVSAENLEQANKMLLEISQLSTPFGTSAQRVAAYFSEAISARLVSSCLGIYATLPPHTLHNQKVASAFQVFNGISPFVKFSHFTANQAIQEAFDREERVHIIDLDIMQGLQWPGLFHILASRPGGPPYVRLTGLGTSMETLEATGKRLSDFASKLGLPFEFFPVAEKVGNIDVEKLNVSKSEAVAVHWLQHSLYDVTGSDTNTLWLLQRLAPKVVTVVEQDLSNAGSFLGRFVEAIHYYSALFDSLGSSYGEESEERHVVEQQLLSREIRNVLAVGGPSRSGEIKFHNWREKLQQCGFRGISLAGNAATQASLLLGMFPSEGYTLVEDNGILKLGWKDLCLLTASAWRPPFHSNNVIPHHN