AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene28357

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene28357.t1 MTR_4g066630 94.589 499 25 1 1 499 1 497 0.0 965
MS.gene28357.t1 MTR_4g066590 88.485 495 53 2 5 499 1 491 0.0 916
MS.gene28357.t1 MTR_4g066630 94.589 462 23 1 1 462 1 460 0.0 889
MS.gene28357.t1 MTR_4g066630 94.601 426 21 1 1 426 1 424 0.0 820
MS.gene28357.t1 MTR_4g066580 73.948 499 128 1 1 499 1 497 0.0 799
MS.gene28357.t1 MTR_3g028720 72.490 498 134 2 3 499 1 496 0.0 779
MS.gene28357.t1 MTR_4g067040 88.049 410 46 1 5 414 1 407 0.0 753
MS.gene28357.t1 MTR_4g066630 93.947 380 21 1 1 380 1 378 0.0 724
MS.gene28357.t1 MTR_2g098540 68.839 491 149 2 9 499 3 489 0.0 721
MS.gene28357.t1 MTR_3g028720 73.043 460 121 2 3 461 1 458 0.0 721
MS.gene28357.t1 MTR_4g067040 87.724 391 45 1 5 395 1 388 0.0 716
MS.gene28357.t1 MTR_2g098790 65.531 499 169 2 1 499 1 496 0.0 708
MS.gene28357.t1 MTR_4g066210 69.264 462 142 0 37 498 39 500 0.0 704
MS.gene28357.t1 MTR_2g098520 68.750 464 145 0 36 499 29 492 0.0 701
MS.gene28357.t1 MTR_6g078450 70.000 460 138 0 40 499 35 494 0.0 700
MS.gene28357.t1 MTR_4g066240 65.914 487 163 1 12 498 10 493 0.0 692
MS.gene28357.t1 MTR_4g066640 65.644 489 162 3 12 499 12 495 0.0 690
MS.gene28357.t1 MTR_4g066240 65.779 488 163 2 12 498 10 494 0.0 687
MS.gene28357.t1 MTR_2g098540 71.136 440 127 0 60 499 6 445 0.0 682
MS.gene28357.t1 MTR_4g066580 73.721 430 111 1 1 430 1 428 0.0 679
MS.gene28357.t1 MTR_6g078330 65.531 499 164 3 5 499 1 495 0.0 673
MS.gene28357.t1 MTR_6g078380 69.631 461 139 1 40 499 35 495 0.0 670
MS.gene28357.t1 MTR_3g028720 73.068 427 112 2 3 428 1 425 0.0 666
MS.gene28357.t1 MTR_6g078450 70.616 422 124 0 40 461 35 456 0.0 645
MS.gene28357.t1 MTR_5g071520 62.525 507 179 2 1 498 1 505 0.0 637
MS.gene28357.t1 MTR_3g026280 60.366 492 178 4 8 499 1 475 0.0 623
MS.gene28357.t1 MTR_4g066340 67.788 416 134 0 84 499 6 421 0.0 613
MS.gene28357.t1 MTR_4g066330 60.765 497 162 5 6 498 7 474 0.0 607
MS.gene28357.t1 MTR_3g028720 72.123 391 106 2 3 392 1 389 0.0 602
MS.gene28357.t1 MTR_4g066280 62.039 461 147 2 40 498 40 474 0.0 595
MS.gene28357.t1 MTR_4g066240 65.783 415 139 1 12 426 10 421 0.0 587
MS.gene28357.t1 MTR_4g023000 59.783 460 179 3 40 499 23 476 0.0 585
MS.gene28357.t1 MTR_6g078330 67.519 391 127 0 109 499 1 391 0.0 562
MS.gene28357.t1 MTR_3g026400 54.845 485 211 5 13 496 11 488 0.0 542
MS.gene28357.t1 MTR_2g043200 58.824 459 155 6 46 498 70 500 0.0 537
MS.gene28357.t1 MTR_4g015420 56.383 470 165 7 41 498 65 506 0.0 535
MS.gene28357.t1 MTR_4g066640 64.752 383 129 3 12 393 12 389 0.0 528
MS.gene28357.t1 MTR_6g078330 64.908 379 131 1 121 499 15 391 0.0 522
MS.gene28357.t1 MTR_6g078330 66.667 375 117 3 5 375 1 371 0.0 518
MS.gene28357.t1 MTR_3g026620 53.279 488 219 5 9 496 11 489 3.14e-178 515
MS.gene28357.t1 MTR_4g015460 54.430 474 178 6 41 504 42 487 4.76e-177 512
MS.gene28357.t1 MTR_3g026400 55.804 448 190 5 50 496 17 457 4.10e-176 509
MS.gene28357.t1 MTR_4g083000 51.129 487 213 5 21 498 14 484 1.85e-174 505
MS.gene28357.t1 MTR_5g044000 50.298 503 221 7 21 498 5 503 3.77e-171 498
MS.gene28357.t1 MTR_0110s0040 47.485 497 255 4 1 497 3 493 9.44e-164 478
MS.gene28357.t1 MTR_0110s0030 47.755 490 245 5 8 497 15 493 1.99e-162 475
MS.gene28357.t1 MTR_4g081530 50.431 464 220 5 40 498 23 481 1.64e-159 468
MS.gene28357.t1 MTR_3g033790 48.478 460 223 5 40 499 18 463 9.50e-155 454
MS.gene28357.t1 MTR_4g131810 49.780 454 224 3 46 496 33 485 1.49e-154 455
MS.gene28357.t1 MTR_3g033810 48.261 460 225 4 40 499 20 466 2.93e-153 451
MS.gene28357.t1 MTR_2g022760 49.666 449 222 3 50 496 45 491 1.59e-150 445
MS.gene28357.t1 MTR_4g131800 48.268 462 234 4 46 505 521 979 8.87e-145 446
MS.gene28357.t1 MTR_3g026400 56.369 369 153 5 13 380 11 372 9.14e-144 422
MS.gene28357.t1 MTR_2g079580 44.586 471 249 5 40 505 31 494 5.35e-143 426
MS.gene28357.t1 MTR_2g079550 45.011 471 247 5 40 505 29 492 1.38e-141 422
MS.gene28357.t1 MTR_2g022710 45.064 466 251 4 40 503 34 496 1.58e-139 417
MS.gene28357.t1 MTR_4g016920 44.488 508 260 8 40 540 26 518 1.12e-138 414
MS.gene28357.t1 MTR_4g083000 50.376 399 180 3 109 498 1 390 5.40e-138 409
MS.gene28357.t1 MTR_7g068910 41.463 533 280 8 1 529 1 505 8.32e-138 412
MS.gene28357.t1 MTR_5g068740 45.877 473 228 8 36 501 22 473 1.42e-137 411
MS.gene28357.t1 MTR_1g088610 46.610 472 225 8 36 501 22 472 6.44e-135 409
MS.gene28357.t1 MTR_7g068910 40.700 543 280 9 1 529 1 515 1.17e-134 404
MS.gene28357.t1 MTR_7g033860 44.731 465 228 7 43 498 50 494 1.48e-131 396
MS.gene28357.t1 MTR_0110s0040 48.158 380 193 3 1 380 3 378 2.39e-124 374
MS.gene28357.t1 MTR_0108s0030 42.325 456 234 4 43 498 5 431 4.69e-123 372
MS.gene28357.t1 MTR_2g079580 46.429 392 198 5 40 426 31 415 2.84e-122 369
MS.gene28357.t1 MTR_2g022710 44.575 424 230 4 40 461 34 454 5.68e-121 367
MS.gene28357.t1 MTR_2g022710 44.575 424 230 4 40 461 34 454 1.06e-120 367
MS.gene28357.t1 MTR_2g022760 47.436 390 201 3 109 496 1 388 2.14e-120 363
MS.gene28357.t1 MTR_7g051610 44.323 458 235 6 42 498 76 514 1.29e-119 366
MS.gene28357.t1 MTR_7g051510 43.326 457 241 5 42 498 76 514 1.61e-118 363
MS.gene28357.t1 MTR_2g022710 46.272 389 204 4 40 426 34 419 1.03e-117 357
MS.gene28357.t1 MTR_7g051650 42.373 472 245 7 42 511 76 522 9.26e-115 353
MS.gene28357.t1 MTR_1g088630 44.420 448 198 10 60 501 14 416 1.70e-114 350
MS.gene28357.t1 MTR_7g051650 41.826 471 245 6 42 511 76 518 1.34e-113 350
MS.gene28357.t1 MTR_2g022710 45.623 377 200 4 40 414 34 407 2.19e-111 340
MS.gene28357.t1 MTR_2g022710 43.073 397 222 3 109 503 1 395 2.33e-111 340
MS.gene28357.t1 MTR_4g016920 42.825 439 230 7 109 540 1 425 2.58e-110 338
MS.gene28357.t1 MTR_7g068900 43.596 406 196 6 104 498 17 400 6.16e-109 337
MS.gene28357.t1 MTR_1g088630 49.432 352 135 7 43 390 29 341 1.53e-107 329
MS.gene28357.t1 MTR_2g022710 49.068 322 161 3 40 361 34 352 3.17e-104 322
MS.gene28357.t1 MTR_7g056747 43.073 397 200 4 103 498 9 380 1.85e-98 306
MS.gene28357.t1 MTR_1g088630 53.160 269 114 4 43 307 29 289 4.88e-94 291
MS.gene28357.t1 MTR_4g016920 53.558 267 121 2 109 374 1 265 1.49e-93 291
MS.gene28357.t1 MTR_0110s0020 49.621 264 124 4 8 271 15 269 3.47e-88 277
MS.gene28357.t1 MTR_4g122980 26.952 397 217 14 105 495 183 512 1.48e-26 115
MS.gene28357.t1 MTR_8g038650 37.879 132 69 2 241 362 24 152 1.55e-22 95.1
MS.gene28357.t1 MTR_8g038650 55.556 54 23 1 50 103 7 59 1.82e-13 68.9
MS.gene28357.t1 MTR_5g069350 42.336 137 51 4 355 481 48 166 3.72e-19 86.3
MS.gene28357.t1 MTR_4g061170 72.500 40 9 1 462 499 22 61 1.14e-12 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene28357.t1 AT5G44640 55.444 496 209 5 5 499 1 485 0.0 579
MS.gene28357.t1 AT5G42260 55.242 496 210 5 5 499 1 485 0.0 575
MS.gene28357.t1 AT2G44450 57.516 459 190 2 41 499 31 484 0.0 572
MS.gene28357.t1 AT2G44480 56.304 460 200 1 40 499 34 492 0.0 559
MS.gene28357.t1 AT2G44480 56.304 460 200 1 40 499 65 523 0.0 559
MS.gene28357.t1 AT3G60130 56.087 460 196 3 41 499 30 484 0.0 554
MS.gene28357.t1 AT2G25630 53.112 482 203 4 18 498 7 466 0.0 541
MS.gene28357.t1 AT5G24550 53.780 463 209 5 41 499 32 493 1.36e-179 520
MS.gene28357.t1 AT5G24540 51.417 494 233 6 12 499 1 493 2.62e-173 503
MS.gene28357.t1 AT3G60140 48.861 483 236 6 20 499 12 486 6.80e-172 501
MS.gene28357.t1 AT1G51470 53.796 461 199 7 40 498 44 492 8.51e-172 499
MS.gene28357.t1 AT1G47600 53.362 461 201 7 40 498 44 492 8.07e-171 496
MS.gene28357.t1 AT1G47600 53.362 461 200 7 40 498 44 491 2.58e-170 495
MS.gene28357.t1 AT3G18080 50.524 477 227 6 24 497 20 490 2.97e-168 490
MS.gene28357.t1 AT1G26560 50.972 463 217 6 41 498 32 489 8.32e-168 489
MS.gene28357.t1 AT5G54570 50.214 468 216 4 40 498 29 488 1.22e-166 486
MS.gene28357.t1 AT2G44460 50.955 471 226 5 29 496 17 485 4.12e-165 484
MS.gene28357.t1 AT3G18070 49.160 476 225 7 32 505 21 481 1.78e-164 479
MS.gene28357.t1 AT3G18070 49.370 476 230 7 32 505 21 487 1.70e-163 477
MS.gene28357.t1 AT2G44470 47.558 471 243 4 31 498 19 488 2.35e-163 480
MS.gene28357.t1 AT3G60120 50.108 463 226 5 40 499 13 473 4.27e-163 478
MS.gene28357.t1 AT2G44490 50.538 465 224 5 40 499 13 476 9.40e-161 473
MS.gene28357.t1 AT2G44480 54.476 391 177 1 109 499 1 390 5.32e-160 465
MS.gene28357.t1 AT3G60130 54.523 398 175 3 103 499 40 432 1.07e-159 466
MS.gene28357.t1 AT5G25980 47.904 501 250 8 3 498 10 504 1.28e-157 464
MS.gene28357.t1 AT5G36890 49.455 459 219 4 42 499 17 463 4.90e-155 455
MS.gene28357.t1 AT5G36890 49.455 459 219 4 42 499 17 463 5.30e-155 455
MS.gene28357.t1 AT5G24540 53.302 424 194 4 41 461 32 454 7.51e-154 451
MS.gene28357.t1 AT5G26000 47.686 497 239 10 13 496 3 491 5.79e-153 452
MS.gene28357.t1 AT3G60130 53.518 398 168 4 103 499 40 421 1.08e-152 448
MS.gene28357.t1 AT3G09260 48.596 463 231 6 40 498 34 493 4.84e-151 446
MS.gene28357.t1 AT5G54570 49.535 430 201 3 40 461 29 450 5.51e-150 441
MS.gene28357.t1 AT2G32860 45.022 462 251 3 40 498 93 554 1.51e-148 443
MS.gene28357.t1 AT2G44470 47.235 434 226 3 31 462 19 451 1.91e-148 437
MS.gene28357.t1 AT2G32860 45.022 462 250 4 40 498 93 553 4.55e-148 442
MS.gene28357.t1 AT3G03640 45.000 500 262 6 3 496 1 493 3.08e-147 437
MS.gene28357.t1 AT3G21370 47.436 468 232 9 40 498 32 494 1.10e-146 435
MS.gene28357.t1 AT1G02850 48.261 460 216 7 42 498 27 467 2.71e-145 431
MS.gene28357.t1 AT1G75940 48.109 476 217 12 41 498 37 500 5.23e-145 431
MS.gene28357.t1 AT3G60140 48.900 409 199 5 20 426 12 412 4.19e-144 426
MS.gene28357.t1 AT1G52400 43.939 528 274 12 12 529 11 526 1.84e-143 427
MS.gene28357.t1 AT1G52400 43.939 528 274 12 12 529 11 526 1.84e-143 427
MS.gene28357.t1 AT5G25980 47.300 463 234 7 3 461 10 466 5.14e-143 424
MS.gene28357.t1 AT5G25980 47.300 463 234 7 3 461 10 466 5.14e-143 424
MS.gene28357.t1 AT5G28510 46.934 473 239 7 35 498 33 502 5.53e-143 426
MS.gene28357.t1 AT1G66280 46.029 491 251 9 16 498 9 493 1.02e-141 422
MS.gene28357.t1 AT1G02850 46.170 483 215 8 42 498 27 490 4.77e-141 421
MS.gene28357.t1 AT1G61820 45.181 498 251 7 3 496 7 486 8.38e-140 417
MS.gene28357.t1 AT1G02850 46.074 484 215 9 42 498 27 491 2.39e-139 416
MS.gene28357.t1 AT1G66270 46.667 465 236 8 40 498 35 493 1.09e-138 414
MS.gene28357.t1 AT5G26000 46.855 461 225 9 13 461 3 455 5.41e-138 410
MS.gene28357.t1 AT4G21760 43.590 507 272 7 4 505 15 512 1.52e-137 412
MS.gene28357.t1 AT2G44480 60.256 312 122 2 40 351 65 374 6.12e-137 405
MS.gene28357.t1 AT1G66270 46.452 465 235 9 40 498 35 491 1.32e-135 407
MS.gene28357.t1 AT1G02850 46.739 460 196 8 42 498 27 440 1.44e-134 402
MS.gene28357.t1 AT4G27830 42.974 491 248 8 13 498 3 466 1.01e-133 401
MS.gene28357.t1 AT5G54570 48.872 399 187 4 109 498 1 391 1.21e-133 399
MS.gene28357.t1 AT2G44450 53.846 338 151 2 162 499 1 333 6.17e-133 394
MS.gene28357.t1 AT1G60090 44.468 470 234 8 41 507 24 469 1.47e-132 398
MS.gene28357.t1 AT4G21760 42.801 507 266 8 4 505 15 502 1.57e-131 397
MS.gene28357.t1 AT1G61810 46.035 454 238 5 46 495 38 488 1.62e-131 397
MS.gene28357.t1 AT1G61810 46.035 454 238 5 46 495 38 488 3.62e-131 395
MS.gene28357.t1 AT4G22100 44.350 469 236 8 42 507 23 469 1.68e-129 390
MS.gene28357.t1 AT1G60090 43.215 479 234 9 41 507 24 476 1.54e-128 388
MS.gene28357.t1 AT2G44470 49.565 345 172 2 31 374 19 362 1.62e-126 379
MS.gene28357.t1 AT1G02850 48.418 411 173 7 42 426 27 424 2.66e-126 380
MS.gene28357.t1 AT1G52400 44.662 459 236 9 12 461 11 460 5.67e-126 380
MS.gene28357.t1 AT3G62750 41.949 472 243 6 30 498 12 455 2.60e-125 379
MS.gene28357.t1 AT3G62750 41.949 472 243 6 30 498 38 481 8.06e-125 379
MS.gene28357.t1 AT4G27820 44.252 461 237 5 40 498 22 464 8.32e-125 378
MS.gene28357.t1 AT1G45191 42.275 466 244 6 42 507 30 470 2.88e-121 369
MS.gene28357.t1 AT3G18070 52.395 334 152 5 32 365 21 347 1.51e-120 363
MS.gene28357.t1 AT5G48375 45.414 447 172 9 37 480 34 411 3.61e-120 364
MS.gene28357.t1 AT3G62740 41.880 468 248 8 33 498 15 460 2.14e-119 364
MS.gene28357.t1 AT1G45191 42.060 466 251 7 42 507 30 476 1.44e-118 362
MS.gene28357.t1 AT1G45191 41.826 471 250 8 42 507 30 481 1.16e-117 360
MS.gene28357.t1 AT1G45191 41.372 481 250 9 42 507 30 493 2.06e-116 357
MS.gene28357.t1 AT3G62750 41.781 438 224 6 30 464 38 447 1.31e-111 343
MS.gene28357.t1 AT3G62740 45.524 391 195 7 38 426 20 394 2.53e-110 338
MS.gene28357.t1 AT1G61810 45.385 390 206 5 41 426 33 419 5.10e-110 337
MS.gene28357.t1 AT1G60270 48.071 337 164 5 41 374 24 352 9.36e-108 330
MS.gene28357.t1 AT1G51490 48.857 350 160 7 48 393 27 361 1.96e-106 328
MS.gene28357.t1 AT3G62750 46.132 349 168 4 30 374 12 344 2.71e-106 326
MS.gene28357.t1 AT3G62750 46.132 349 168 4 30 374 12 344 2.71e-106 326
MS.gene28357.t1 AT3G62750 46.132 349 168 4 30 374 38 370 1.34e-105 326
MS.gene28357.t1 AT1G45191 48.624 327 157 4 42 365 30 348 7.64e-105 323
MS.gene28357.t1 AT1G45191 48.624 327 157 4 42 365 30 348 1.30e-104 323
MS.gene28357.t1 AT1G61810 44.246 391 211 5 109 495 1 388 5.01e-104 322
MS.gene28357.t1 AT2G44460 49.412 340 168 4 160 496 1 339 1.09e-103 321
MS.gene28357.t1 AT4G27830 42.239 393 209 5 109 498 1 378 5.96e-103 319
MS.gene28357.t1 AT2G44480 50.709 282 138 1 218 499 6 286 1.95e-98 303
MS.gene28357.t1 AT3G18070 47.152 316 161 4 192 505 99 410 6.44e-98 306
MS.gene28357.t1 AT3G18070 56.944 72 30 1 32 103 21 91 3.88e-19 90.9
MS.gene28357.t1 AT4G27820 42.347 392 211 4 109 498 1 379 1.87e-95 300
MS.gene28357.t1 AT4G27820 42.347 392 211 4 109 498 1 379 1.87e-95 300
MS.gene28357.t1 AT3G62750 39.440 393 212 5 109 498 1 370 2.50e-92 291
MS.gene28357.t1 AT3G62750 39.440 393 212 5 109 498 1 370 2.50e-92 291
MS.gene28357.t1 AT1G61820 44.798 346 185 4 155 496 4 347 2.57e-92 290
MS.gene28357.t1 AT3G18070 46.358 302 156 4 206 505 1 298 6.83e-91 284
MS.gene28357.t1 AT1G61810 51.111 270 130 2 41 310 33 300 1.82e-89 280
MS.gene28357.t1 AT1G60270 46.377 276 141 4 109 381 1 272 2.77e-78 250
MS.gene28357.t1 AT3G18070 48.319 238 119 3 193 428 100 335 2.22e-72 237
MS.gene28357.t1 AT3G18070 56.944 72 30 1 32 103 21 91 1.84e-19 90.9
MS.gene28357.t1 AT3G18070 47.321 224 114 3 206 427 1 222 1.60e-65 215
MS.gene28357.t1 AT5G16580 36.486 296 167 6 181 472 21 299 6.58e-48 170
MS.gene28357.t1 AT3G06510 25.359 418 233 16 104 495 159 523 3.50e-26 114
MS.gene28357.t1 AT3G06510 25.000 404 218 14 104 495 159 489 5.04e-26 113

Find 119 sgRNAs with CRISPR-Local

Find 190 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCCAGAAGGCTTCATATT+TGG 0.217301 4.2:+38858293 MS.gene28357:CDS
CTTGTTTAATTATAGGTTTA+TGG 0.235433 4.2:+38859553 MS.gene28357:intron
CACCAGGTCGATGTTCTTCT+TGG 0.235520 4.2:+38859117 MS.gene28357:CDS
AATTGCACCGGTGGTGATTC+TGG 0.263758 4.2:+38859149 MS.gene28357:CDS
ACCATCCATATTAACCATTA+TGG 0.266450 4.2:-38861229 None:intergenic
GAATCACCACCGGTGCAATT+TGG 0.269114 4.2:-38859146 None:intergenic
AAGTATCAAGTTTCTCAAAA+TGG 0.272356 4.2:+38859242 MS.gene28357:CDS
GTCCTCCATAATGGTTAATA+TGG 0.292343 4.2:+38861224 MS.gene28357:CDS
GTGGAAGAGGACCAAGTATA+TGG 0.292452 4.2:+38858360 MS.gene28357:CDS
CAATAACCTCATTGATGAAT+TGG 0.307366 4.2:+38858682 MS.gene28357:CDS
TGATAGTGTGCAGTTAAATA+AGG 0.310074 4.2:-38859179 None:intergenic
GAACTTTGCTTTAAAACATT+TGG 0.321295 4.2:+38859017 MS.gene28357:CDS
AAGACAACAATAGAGAAAAT+AGG 0.342774 4.2:-38858181 None:intergenic
AGGGGTTATACTGTGAGATT+TGG 0.352676 4.2:+38860467 MS.gene28357:CDS
GTGCCAAATATGAAGCCTTC+TGG 0.376589 4.2:-38858296 None:intergenic
AGACCCAAGCAAGGCTTACT+TGG 0.376594 4.2:-38861330 None:intergenic
CGAGCTACAGACTTTCAGTT+TGG 0.379816 4.2:+38859341 MS.gene28357:CDS
AAACTTACATTTAGACCAAT+TGG 0.381135 4.2:-38859901 None:intergenic
CCATGGAGTTACAGTCAAAA+TGG 0.382192 4.2:+38859074 MS.gene28357:CDS
TGCAAATAGGTTCATTGAAT+AGG 0.394366 4.2:+38858264 MS.gene28357:CDS
TTAATTTATATTACTGAAAA+CGG 0.394477 4.2:+38860096 MS.gene28357:CDS
GTATAACATTGATCATTAAC+TGG 0.399683 4.2:+38859273 MS.gene28357:CDS
CAATCTCTAGAAGATGAATA+TGG 0.407604 4.2:+38858855 MS.gene28357:CDS
TATCTCATGCTTAATGTCAT+AGG 0.409360 4.2:-38858155 None:intergenic
AACGCTGCACCACTGCAAAT+AGG 0.417516 4.2:+38858251 MS.gene28357:CDS
AAATGTTGGGTCCTCCATAA+TGG 0.421142 4.2:+38861215 MS.gene28357:CDS
GATTGCTCTTTAGTAAACTT+TGG 0.424137 4.2:-38859635 None:intergenic
CTCAGCCTCATTGAAGGAAT+AGG 0.434784 4.2:-38861141 None:intergenic
GAAATTAGTACTATCAAAAC+AGG 0.439916 4.2:+38861273 MS.gene28357:CDS
TTATAGGTTTATGGATCCCT+TGG 0.446664 4.2:+38859562 MS.gene28357:intron
AGCATAGGAGTAGTAGCATA+TGG 0.447074 4.2:-38861359 None:intergenic
TGACTCGTATCATCGATATA+AGG 0.447189 4.2:+38858445 MS.gene28357:CDS
CCTTCCCTCAGCCTCATTGA+AGG 0.453743 4.2:-38861147 None:intergenic
CAGAGAATCTGTTAGGTAGC+TGG 0.461134 4.2:-38859750 None:intergenic
TATTCATCTTCTAGAGATTG+AGG 0.466417 4.2:-38858852 None:intergenic
GATGTTGAAACAGATGCCTT+GGG 0.469707 4.2:+38861419 MS.gene28357:CDS
GTTTCTCAAAATGGTTTGAT+CGG 0.472343 4.2:+38859251 MS.gene28357:CDS
GCACGAGATGGAAAACCAAT+TGG 0.474977 4.2:+38859886 MS.gene28357:CDS
GTACGACGATGCATCATTAT+CGG 0.478412 4.2:+38860214 MS.gene28357:CDS
TGATGTTGAAACAGATGCCT+TGG 0.484122 4.2:+38861418 MS.gene28357:CDS
GAATTTGCATTGCTTAATTG+AGG 0.486109 4.2:-38859728 None:intergenic
GCAGCAAGTGAAGGTGGAAG+AGG 0.487766 4.2:+38858347 MS.gene28357:CDS
AACTAGACAGAGAATCTGTT+AGG 0.488098 4.2:-38859757 None:intergenic
ATACTTTGCATGGTCTCTGT+TGG 0.489827 4.2:+38860427 MS.gene28357:CDS
GAGGCCAAGTAAGCCTTGCT+TGG 0.490078 4.2:+38861326 MS.gene28357:CDS
GAGTATTCTAGACCAAGATA+TGG 0.494023 4.2:-38858516 None:intergenic
CAGGGCTGGTGTAAATGTAA+AGG 0.496998 4.2:+38860403 MS.gene28357:intron
AATTTCAGGTTGCTTCAGAA+TGG 0.500231 4.2:+38860007 MS.gene28357:intron
CTACAGACTTTCAGTTTGGA+TGG 0.501459 4.2:+38859345 MS.gene28357:CDS
CCACATGTGATAAGGCGTAT+AGG 0.504126 4.2:+38861087 MS.gene28357:CDS
AGTCAAAATGGATATGCAAA+TGG 0.504705 4.2:+38859086 MS.gene28357:CDS
GTGTGCAGTTAAATAAGGTT+CGG 0.508262 4.2:-38859174 None:intergenic
CATCGATATAAGGAAGATGT+TGG 0.511755 4.2:+38858455 MS.gene28357:CDS
AGGCCAAGTAAGCCTTGCTT+GGG 0.512930 4.2:+38861327 MS.gene28357:CDS
CTGAGGGAAGGGACTATGAA+TGG 0.514749 4.2:+38861159 MS.gene28357:CDS
TTCATTATTACTTTAACAGA+AGG 0.515348 4.2:+38858215 MS.gene28357:CDS
AGGGCTGGTGTAAATGTAAA+GGG 0.516329 4.2:+38860404 MS.gene28357:intron
CTATTCCTTCAATGAGGCTG+AGG 0.516412 4.2:+38861142 MS.gene28357:CDS
TGGATAATCTCCATTGGCCA+AGG 0.518626 4.2:-38859579 None:intergenic
ATGCTGGTAAATCAAATGTT+GGG 0.521252 4.2:+38861202 MS.gene28357:CDS
TCCATAATGGTTAATATGGA+TGG 0.523669 4.2:+38861228 MS.gene28357:CDS
GCAAATGGCGCAATGGCACC+AGG 0.525181 4.2:+38859101 MS.gene28357:CDS
CTTCAATGAGGCTGAGGGAA+GGG 0.525219 4.2:+38861148 MS.gene28357:CDS
ACTACTCCTATGCTTACAAA+TGG 0.525652 4.2:+38861368 MS.gene28357:CDS
TGGAAGAGGACCAAGTATAT+GGG 0.531156 4.2:+38858361 MS.gene28357:CDS
AATGCTGGTAAATCAAATGT+TGG 0.538243 4.2:+38861201 MS.gene28357:CDS
ATTGATGAATTGGTGGCAAA+TGG 0.539277 4.2:+38858692 MS.gene28357:CDS
TAACCTCATTGATGAATTGG+TGG 0.539436 4.2:+38858685 MS.gene28357:CDS
TGATCTTACCAATGCTCGCA+TGG 0.539778 4.2:-38859606 None:intergenic
CGATGCATCATTATCGGTTG+AGG 0.542716 4.2:+38860220 MS.gene28357:CDS
ATGTGTTGCCACATGTGATA+AGG 0.542786 4.2:+38861079 MS.gene28357:CDS
TCTTCTGCATATCAATATGA+AGG 0.549189 4.2:+38858323 MS.gene28357:CDS
TTTATGGATCCCTTGGCCAA+TGG 0.549813 4.2:+38859569 MS.gene28357:CDS
GTTCAGAATGTTGCAAATGC+TGG 0.555235 4.2:+38861186 MS.gene28357:CDS
GGATAACTTTGAACGAGCCA+TGG 0.555448 4.2:+38859057 MS.gene28357:CDS
TTGGAGATAGAGTAAAACAT+TGG 0.559088 4.2:+38859036 MS.gene28357:CDS
GTGATTGCAGTCGCACGAGA+TGG 0.559174 4.2:+38859874 MS.gene28357:intron
TATCCTCAAAAGATCAGTGA+TGG 0.559832 4.2:+38858401 MS.gene28357:CDS
GTGAAAGTATCCCATATACT+TGG 0.569026 4.2:-38858371 None:intergenic
TTGCCACCAATTCATCAATG+AGG 0.575333 4.2:-38858688 None:intergenic
AAGATCAGTGATGGAAACAA+TGG 0.577375 4.2:+38858410 MS.gene28357:CDS
GAGCAATCTAAGCAAGTCAA+AGG 0.581133 4.2:+38859650 MS.gene28357:CDS
GGATAATCTCCATTGGCCAA+GGG 0.583402 4.2:-38859578 None:intergenic
TTCAATGAACCTATTTGCAG+TGG 0.584960 4.2:-38858260 None:intergenic
TGGATATGCAAATGGCGCAA+TGG 0.586330 4.2:+38859094 MS.gene28357:CDS
AATCATGAAGGATATGAACT+TGG 0.589040 4.2:+38858478 MS.gene28357:CDS
GGAAGATGTTGGAATCATGA+AGG 0.590169 4.2:+38858466 MS.gene28357:CDS
TGGTTGAATCCAAATTGCAC+CGG 0.592901 4.2:+38859137 MS.gene28357:CDS
AGTGGTGGAATTAATCAAGA+AGG 0.596031 4.2:+38858647 MS.gene28357:CDS
GAAGGTGCAGCAAGTGAAGG+TGG 0.597164 4.2:+38858341 MS.gene28357:CDS
CCTTCAATGAGGCTGAGGGA+AGG 0.597841 4.2:+38861147 MS.gene28357:CDS
AGGATCTTGAAGACACATGT+TGG 0.598388 4.2:-38861121 None:intergenic
CTTTAAGATAAAAGAGATGA+CGG 0.600540 4.2:-38860280 None:intergenic
TATGAAGGTGCAGCAAGTGA+AGG 0.604375 4.2:+38858338 MS.gene28357:CDS
CTTCTTCAGTTCAATCCCCA+AGG 0.607731 4.2:-38861435 None:intergenic
GCAGAAGGAAAACTAAGTGG+TGG 0.613240 4.2:+38858632 MS.gene28357:intron
AAGATCCTATTCCTTCAATG+AGG 0.617508 4.2:+38861136 MS.gene28357:CDS
TTCGGTTCCAGAATCACCAC+CGG 0.620495 4.2:-38859156 None:intergenic
TGGTTGTATGTTTATCCAAG+AGG 0.620982 4.2:+38860027 MS.gene28357:CDS
TGCACACCATTTGTAAGCAT+AGG 0.622179 4.2:-38861374 None:intergenic
TTATGTTTGTTATCTGAGAG+CGG 0.625110 4.2:-38859302 None:intergenic
ATGTAAAGGGATACTTTGCA+TGG 0.626161 4.2:+38860417 MS.gene28357:CDS
ATCCATATTAACCATTATGG+AGG 0.631731 4.2:-38861226 None:intergenic
TATGCAGAAGGAAAACTAAG+TGG 0.632774 4.2:+38858629 MS.gene28357:intron
TATTCCTTCAATGAGGCTGA+GGG 0.633853 4.2:+38861143 MS.gene28357:CDS
ACATCAAGAACAACTTGACA+TGG 0.642250 4.2:-38861401 None:intergenic
CCTATACGCCTTATCACATG+TGG 0.649578 4.2:-38861087 None:intergenic
GAATGCTTAGTTGAGCAACA+AGG 0.651866 4.2:-38861299 None:intergenic
CTCAACTAAGCATTCTCACG+AGG 0.652339 4.2:+38861307 MS.gene28357:CDS
TGTATCACTCACATGATGAG+AGG 0.655919 4.2:-38858891 None:intergenic
CAAACATAATCAAAATGCTG+CGG 0.660707 4.2:+38859316 MS.gene28357:CDS
TGGAAGCATAGACCCAAGCA+AGG 0.668896 4.2:-38861339 None:intergenic
TTGAATCCAAATTGCACCGG+TGG 0.672179 4.2:+38859140 MS.gene28357:CDS
AACCAAGAAGAACATCGACC+TGG 0.677559 4.2:-38859119 None:intergenic
TCTCTAGAAGATGAATATGG+CGG 0.701636 4.2:+38858858 MS.gene28357:CDS
ATGTTGAAACAGATGCCTTG+GGG 0.703166 4.2:+38861420 MS.gene28357:CDS
TGGAAACAATGGAGACGTTG+CGG 0.708998 4.2:+38858421 MS.gene28357:CDS
TATGGATGGTCGTATCAATG+CGG 0.767395 4.2:+38861242 MS.gene28357:CDS
AAAAGACTTTCAAAACTACG+CGG 0.803991 4.2:+38858995 MS.gene28357:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAAAAAAAATAAAAAATA+TGG - chr4.2:38858604-38858623 None:intergenic 0.0%
!! AAAAAAATAAAAAATATGGT+TGG - chr4.2:38858600-38858619 None:intergenic 10.0%
!! AGTATTATAAATTTAAATGA+TGG + chr4.2:38859835-38859854 MS.gene28357:intron 10.0%
!! TTAATTTATATTACTGAAAA+CGG + chr4.2:38860096-38860115 MS.gene28357:CDS 10.0%
!!! AATTTAGAATAATATACTTT+AGG - chr4.2:38860930-38860949 None:intergenic 10.0%
!!! TTTTCAGTAATATAAATTAA+AGG - chr4.2:38860096-38860115 None:intergenic 10.0%
!! TTCATTGTTTAAATATAAGA+AGG + chr4.2:38860672-38860691 MS.gene28357:intron 15.0%
!!! CATCTTACTTTTTTTTTTTT+TGG + chr4.2:38859942-38859961 MS.gene28357:intron 15.0%
!!! TTTTTTATAATTTATGCAGA+AGG + chr4.2:38858617-38858636 MS.gene28357:intron 15.0%
!! ACCTATAATTAAACAAGAAA+TGG - chr4.2:38859550-38859569 None:intergenic 20.0%
!! ACTTATATTCAGTTTAATAG+TGG - chr4.2:38861012-38861031 None:intergenic 20.0%
!! CTTGTTTAATTATAGGTTTA+TGG + chr4.2:38859553-38859572 MS.gene28357:intron 20.0%
!! TCTTATGTAAAAAAAATTGC+AGG + chr4.2:38858792-38858811 MS.gene28357:intron 20.0%
!! TGATTACAAAAAATCTACTA+GGG + chr4.2:38860829-38860848 MS.gene28357:intron 20.0%
!! TTCATTATTACTTTAACAGA+AGG + chr4.2:38858215-38858234 MS.gene28357:CDS 20.0%
!! TTTAATTGAGAATAGTCAAT+TGG - chr4.2:38858562-38858581 None:intergenic 20.0%
!!! AAAGGTTCATTTGATTTTAT+TGG + chr4.2:38859668-38859687 MS.gene28357:CDS 20.0%
!!! AATATGGTTTATACCTTTTA+AGG + chr4.2:38860893-38860912 MS.gene28357:intron 20.0%
!!! ATTTGTAACTCTTTTTCATT+GGG + chr4.2:38858824-38858843 MS.gene28357:CDS 20.0%
!!! ATTTTCATAGATTACAAAGA+TGG + chr4.2:38860494-38860513 MS.gene28357:CDS 20.0%
!!! GCAATTTTTTTTACATAAGA+CGG - chr4.2:38858792-38858811 None:intergenic 20.0%
!!! GGAATTATTTTTTCCTTAAA+AGG - chr4.2:38860909-38860928 None:intergenic 20.0%
!!! TAATTCAAATTTTCATGTGT+AGG + chr4.2:38860182-38860201 MS.gene28357:intron 20.0%
!!! TCCATTTCTTGTTTAATTAT+AGG + chr4.2:38859546-38859565 MS.gene28357:intron 20.0%
!!! TGTTATGTGTATTTAATTTC+AGG + chr4.2:38859993-38860012 MS.gene28357:intron 20.0%
!!! TTTTGATAGTACTAATTTCA+TGG - chr4.2:38861272-38861291 None:intergenic 20.0%
! AAAAATTCTTCAGTTTGTGA+AGG - chr4.2:38860792-38860811 None:intergenic 25.0%
! AAAATTCTTCAGTTTGTGAA+GGG - chr4.2:38860791-38860810 None:intergenic 25.0%
! AAACTTACATTTAGACCAAT+TGG - chr4.2:38859904-38859923 None:intergenic 25.0%
! AAGACAACAATAGAGAAAAT+AGG - chr4.2:38858184-38858203 None:intergenic 25.0%
! AAGTATCAAGTTTCTCAAAA+TGG + chr4.2:38859242-38859261 MS.gene28357:CDS 25.0%
! ATTTCTTTCAATGAAATGCA+GGG + chr4.2:38860385-38860404 MS.gene28357:intron 25.0%
! CAATGAAAAAGAGTTACAAA+TGG - chr4.2:38858825-38858844 None:intergenic 25.0%
! GAAATTAGTACTATCAAAAC+AGG + chr4.2:38861273-38861292 MS.gene28357:CDS 25.0%
! GTATAACATTGATCATTAAC+TGG + chr4.2:38859273-38859292 MS.gene28357:CDS 25.0%
! GTGATTACAAAAAATCTACT+AGG + chr4.2:38860828-38860847 MS.gene28357:intron 25.0%
! TAATCACAACTTGGTTAAAA+TGG - chr4.2:38860815-38860834 None:intergenic 25.0%
! TAGATATGCTTGTGTTAATT+TGG - chr4.2:38859485-38859504 None:intergenic 25.0%
! TATTTCTTTCAATGAAATGC+AGG + chr4.2:38860384-38860403 MS.gene28357:intron 25.0%
! TCAAACATGAATATACCATT+AGG + chr4.2:38860862-38860881 MS.gene28357:intron 25.0%
!! AATTTTGTAGCACTGTTAAA+TGG - chr4.2:38860600-38860619 None:intergenic 25.0%
!! CATATAGATTTTCCATATCT+TGG + chr4.2:38858504-38858523 MS.gene28357:CDS 25.0%
!! GGTATAAAATTGACTTACTT+GGG - chr4.2:38858541-38858560 None:intergenic 25.0%
!! TCATCTCTTTTATCTTAAAG+AGG + chr4.2:38860283-38860302 MS.gene28357:CDS 25.0%
!! TGGTATAAAATTGACTTACT+TGG - chr4.2:38858542-38858561 None:intergenic 25.0%
!!! AGTCTATTTCATTTTTTGCA+TGG + chr4.2:38860343-38860362 MS.gene28357:intron 25.0%
!!! ATTTTGAGAAACTTGATACT+TGG - chr4.2:38859243-38859262 None:intergenic 25.0%
!!! CATTTGTAACTCTTTTTCAT+TGG + chr4.2:38858823-38858842 MS.gene28357:CDS 25.0%
!!! CTTTAAGATAAAAGAGATGA+CGG - chr4.2:38860283-38860302 None:intergenic 25.0%
!!! GAACTTTGCTTTAAAACATT+TGG + chr4.2:38859017-38859036 MS.gene28357:CDS 25.0%
!!! GATTTTTTGTAATCACAACT+TGG - chr4.2:38860824-38860843 None:intergenic 25.0%
AAAAGACTTTCAAAACTACG+CGG + chr4.2:38858995-38859014 MS.gene28357:CDS 30.0%
AAAAGATATCAAAAGCTCTC+AGG + chr4.2:38860521-38860540 MS.gene28357:intron 30.0%
AATATAAGTTCGACAGATGA+GGG + chr4.2:38861023-38861042 MS.gene28357:intron 30.0%
AATCATGAAGGATATGAACT+TGG + chr4.2:38858478-38858497 MS.gene28357:CDS 30.0%
AATGCTGGTAAATCAAATGT+TGG + chr4.2:38861201-38861220 MS.gene28357:CDS 30.0%
AATTATGTAAGTGACACAGT+CGG - chr4.2:38859455-38859474 None:intergenic 30.0%
ACCATCCATATTAACCATTA+TGG - chr4.2:38861232-38861251 None:intergenic 30.0%
AGATGGAAAAGAAACAAGTT+TGG - chr4.2:38859411-38859430 None:intergenic 30.0%
AGTCAAAATGGATATGCAAA+TGG + chr4.2:38859086-38859105 MS.gene28357:CDS 30.0%
ATCCATATTAACCATTATGG+AGG - chr4.2:38861229-38861248 None:intergenic 30.0%
ATGCTGGTAAATCAAATGTT+GGG + chr4.2:38861202-38861221 MS.gene28357:CDS 30.0%
CAAACATAATCAAAATGCTG+CGG + chr4.2:38859316-38859335 MS.gene28357:CDS 30.0%
CAATAACCTCATTGATGAAT+TGG + chr4.2:38858682-38858701 MS.gene28357:CDS 30.0%
GAATTTGCATTGCTTAATTG+AGG - chr4.2:38859731-38859750 None:intergenic 30.0%
GATTGCTCTTTAGTAAACTT+TGG - chr4.2:38859638-38859657 None:intergenic 30.0%
GTTTCTCAAAATGGTTTGAT+CGG + chr4.2:38859251-38859270 MS.gene28357:CDS 30.0%
TCCATAATGGTTAATATGGA+TGG + chr4.2:38861228-38861247 MS.gene28357:CDS 30.0%
TCTTCTGCATATCAATATGA+AGG + chr4.2:38858323-38858342 MS.gene28357:CDS 30.0%
TGCAAATAGGTTCATTGAAT+AGG + chr4.2:38858264-38858283 MS.gene28357:CDS 30.0%
TGTGTTTACAACAACAACTA+TGG - chr4.2:38860634-38860653 None:intergenic 30.0%
TTAATCTCGAATAACCTTTG+AGG + chr4.2:38861054-38861073 MS.gene28357:intron 30.0%
TTGGAGATAGAGTAAAACAT+TGG + chr4.2:38859036-38859055 MS.gene28357:CDS 30.0%
! AATAGGAATGATTTTCCAGA+AGG + chr4.2:38858281-38858300 MS.gene28357:CDS 30.0%
! CAATCTCTAGAAGATGAATA+TGG + chr4.2:38858855-38858874 MS.gene28357:CDS 30.0%
! CTCTGTTGGATAATTTTGAA+TGG + chr4.2:38860441-38860460 MS.gene28357:CDS 30.0%
! TATGCTTGTGTTAATTTGGA+TGG - chr4.2:38859481-38859500 None:intergenic 30.0%
! TATTCATCTTCTAGAGATTG+AGG - chr4.2:38858855-38858874 None:intergenic 30.0%
! TGATAGTGTGCAGTTAAATA+AGG - chr4.2:38859182-38859201 None:intergenic 30.0%
! TTGAAAGTCTTTTCTGTAGT+TGG - chr4.2:38858988-38859007 None:intergenic 30.0%
! TTTACATAAGACGGCTATTT+TGG - chr4.2:38858783-38858802 None:intergenic 30.0%
!! TTATGTTTGTTATCTGAGAG+CGG - chr4.2:38859305-38859324 None:intergenic 30.0%
AAAAAGTCTCGAATTCCTCT+TGG - chr4.2:38860045-38860064 None:intergenic 35.0%
AACTAGACAGAGAATCTGTT+AGG - chr4.2:38859760-38859779 None:intergenic 35.0%
AAGATCCTATTCCTTCAATG+AGG + chr4.2:38861136-38861155 MS.gene28357:CDS 35.0%
ACATCAAGAACAACTTGACA+TGG - chr4.2:38861404-38861423 None:intergenic 35.0%
ACTACTCCTATGCTTACAAA+TGG + chr4.2:38861368-38861387 MS.gene28357:CDS 35.0%
ATGTAAAGGGATACTTTGCA+TGG + chr4.2:38860417-38860436 MS.gene28357:CDS 35.0%
CATCGATATAAGGAAGATGT+TGG + chr4.2:38858455-38858474 MS.gene28357:CDS 35.0%
CCATTAGGCATCTCAAAATA+TGG + chr4.2:38860877-38860896 MS.gene28357:intron 35.0%
GAATATAAGTTCGACAGATG+AGG + chr4.2:38861022-38861041 MS.gene28357:intron 35.0%
GAGTATTCTAGACCAAGATA+TGG - chr4.2:38858519-38858538 None:intergenic 35.0%
GTCCTCCATAATGGTTAATA+TGG + chr4.2:38861224-38861243 MS.gene28357:CDS 35.0%
GTGTGCAGTTAAATAAGGTT+CGG - chr4.2:38859177-38859196 None:intergenic 35.0%
TAACCTCATTGATGAATTGG+TGG + chr4.2:38858685-38858704 MS.gene28357:CDS 35.0%
TATCCTCAAAAGATCAGTGA+TGG + chr4.2:38858401-38858420 MS.gene28357:CDS 35.0%
TATGCAGAAGGAAAACTAAG+TGG + chr4.2:38858629-38858648 MS.gene28357:intron 35.0%
TGACTCGTATCATCGATATA+AGG + chr4.2:38858445-38858464 MS.gene28357:CDS 35.0%
TGGTTGTATGTTTATCCAAG+AGG + chr4.2:38860027-38860046 MS.gene28357:CDS 35.0%
TTATAGGTTTATGGATCCCT+TGG + chr4.2:38859562-38859581 MS.gene28357:intron 35.0%
TTCAATGAACCTATTTGCAG+TGG - chr4.2:38858263-38858282 None:intergenic 35.0%
TTTCCAGAAGGCTTCATATT+TGG + chr4.2:38858293-38858312 MS.gene28357:CDS 35.0%
! AAAGTCTTTTCTGTAGTTGG+AGG - chr4.2:38858985-38859004 None:intergenic 35.0%
! AAGATCAGTGATGGAAACAA+TGG + chr4.2:38858410-38858429 MS.gene28357:CDS 35.0%
! AGTGGTGGAATTAATCAAGA+AGG + chr4.2:38858647-38858666 MS.gene28357:CDS 35.0%
! ATTGATGAATTGGTGGCAAA+TGG + chr4.2:38858692-38858711 MS.gene28357:CDS 35.0%
! GATAATTTTGAATGGCACAG+GGG + chr4.2:38860449-38860468 MS.gene28357:CDS 35.0%
! GATTGTTTTCCTCAGGTTTT+AGG - chr4.2:38860987-38861006 None:intergenic 35.0%
! GGATAATTTTGAATGGCACA+GGG + chr4.2:38860448-38860467 MS.gene28357:CDS 35.0%
! TCTCTAGAAGATGAATATGG+CGG + chr4.2:38858858-38858877 MS.gene28357:CDS 35.0%
! TGGATAATTTTGAATGGCAC+AGG + chr4.2:38860447-38860466 MS.gene28357:CDS 35.0%
! TTTCCATCACTGATCTTTTG+AGG - chr4.2:38858407-38858426 None:intergenic 35.0%
!! AATTTCAGGTTGCTTCAGAA+TGG + chr4.2:38860007-38860026 MS.gene28357:intron 35.0%
!! GGTTTTTGGATAATCTCCAT+TGG - chr4.2:38859588-38859607 None:intergenic 35.0%
!! GTGAAAGTATCCCATATACT+TGG - chr4.2:38858374-38858393 None:intergenic 35.0%
!!! CCATATTTTGAGATGCCTAA+TGG - chr4.2:38860880-38860899 None:intergenic 35.0%
AAATGTTGGGTCCTCCATAA+TGG + chr4.2:38861215-38861234 MS.gene28357:CDS 40.0%
AGCATAGGAGTAGTAGCATA+TGG - chr4.2:38861362-38861381 None:intergenic 40.0%
AGGATCTTGAAGACACATGT+TGG - chr4.2:38861124-38861143 None:intergenic 40.0%
AGGGGTTATACTGTGAGATT+TGG + chr4.2:38860467-38860486 MS.gene28357:CDS 40.0%
ATACTTTGCATGGTCTCTGT+TGG + chr4.2:38860427-38860446 MS.gene28357:CDS 40.0%
ATCAAAAGCTCTCAGGATTG+TGG + chr4.2:38860528-38860547 MS.gene28357:intron 40.0%
ATGTGTTGCCACATGTGATA+AGG + chr4.2:38861079-38861098 MS.gene28357:CDS 40.0%
ATGTTGAAACAGATGCCTTG+GGG + chr4.2:38861420-38861439 MS.gene28357:CDS 40.0%
CCATGGAGTTACAGTCAAAA+TGG + chr4.2:38859074-38859093 MS.gene28357:CDS 40.0%
CTACAGACTTTCAGTTTGGA+TGG + chr4.2:38859345-38859364 MS.gene28357:CDS 40.0%
GAATGCTTAGTTGAGCAACA+AGG - chr4.2:38861302-38861321 None:intergenic 40.0%
GACTTACAACCTAAAACCTG+AGG + chr4.2:38860975-38860994 MS.gene28357:intron 40.0%
GAGCAATCTAAGCAAGTCAA+AGG + chr4.2:38859650-38859669 MS.gene28357:CDS 40.0%
GATGTTGAAACAGATGCCTT+GGG + chr4.2:38861419-38861438 MS.gene28357:CDS 40.0%
GGAAGATGTTGGAATCATGA+AGG + chr4.2:38858466-38858485 MS.gene28357:CDS 40.0%
GTACGACGATGCATCATTAT+CGG + chr4.2:38860214-38860233 MS.gene28357:CDS 40.0%
GTTCAGAATGTTGCAAATGC+TGG + chr4.2:38861186-38861205 MS.gene28357:CDS 40.0%
TATGGATGGTCGTATCAATG+CGG + chr4.2:38861242-38861261 MS.gene28357:CDS 40.0%
TATTCCTTCAATGAGGCTGA+GGG + chr4.2:38861143-38861162 MS.gene28357:CDS 40.0%
TGATGTTGAAACAGATGCCT+TGG + chr4.2:38861418-38861437 MS.gene28357:CDS 40.0%
TGCACACCATTTGTAAGCAT+AGG - chr4.2:38861377-38861396 None:intergenic 40.0%
TGGAAGAGGACCAAGTATAT+GGG + chr4.2:38858361-38858380 MS.gene28357:CDS 40.0%
TGGTTGAATCCAAATTGCAC+CGG + chr4.2:38859137-38859156 MS.gene28357:CDS 40.0%
TGTATCACTCACATGATGAG+AGG - chr4.2:38858894-38858913 None:intergenic 40.0%
TTGCCACCAATTCATCAATG+AGG - chr4.2:38858691-38858710 None:intergenic 40.0%
! AGGGCTGGTGTAAATGTAAA+GGG + chr4.2:38860404-38860423 MS.gene28357:intron 40.0%
!!! CCATTTTGACTGTAACTCCA+TGG - chr4.2:38859077-38859096 None:intergenic 40.0%
AACCAAGAAGAACATCGACC+TGG - chr4.2:38859122-38859141 None:intergenic 45.0%
CAGAGAATCTGTTAGGTAGC+TGG - chr4.2:38859753-38859772 None:intergenic 45.0%
CCACATGTGATAAGGCGTAT+AGG + chr4.2:38861087-38861106 MS.gene28357:CDS 45.0%
CCTATACGCCTTATCACATG+TGG - chr4.2:38861090-38861109 None:intergenic 45.0%
CGAGCTACAGACTTTCAGTT+TGG + chr4.2:38859341-38859360 MS.gene28357:CDS 45.0%
CTATTCCTTCAATGAGGCTG+AGG + chr4.2:38861142-38861161 MS.gene28357:CDS 45.0%
CTCAACTAAGCATTCTCACG+AGG + chr4.2:38861307-38861326 MS.gene28357:CDS 45.0%
CTCAGCCTCATTGAAGGAAT+AGG - chr4.2:38861144-38861163 None:intergenic 45.0%
CTTTCAATGAAATGCAGGGC+TGG + chr4.2:38860389-38860408 MS.gene28357:intron 45.0%
GCACGAGATGGAAAACCAAT+TGG + chr4.2:38859886-38859905 MS.gene28357:CDS 45.0%
GCAGAAGGAAAACTAAGTGG+TGG + chr4.2:38858632-38858651 MS.gene28357:intron 45.0%
GGATAACTTTGAACGAGCCA+TGG + chr4.2:38859057-38859076 MS.gene28357:CDS 45.0%
GGATAATCTCCATTGGCCAA+GGG - chr4.2:38859581-38859600 None:intergenic 45.0%
GTGCCAAATATGAAGCCTTC+TGG - chr4.2:38858299-38858318 None:intergenic 45.0%
GTGGAAGAGGACCAAGTATA+TGG + chr4.2:38858360-38858379 MS.gene28357:CDS 45.0%
TCCAAAAACCATGCGAGCAT+TGG + chr4.2:38859598-38859617 MS.gene28357:CDS 45.0%
TGATCTTACCAATGCTCGCA+TGG - chr4.2:38859609-38859628 None:intergenic 45.0%
TGGAAACAATGGAGACGTTG+CGG + chr4.2:38858421-38858440 MS.gene28357:CDS 45.0%
TGGATAATCTCCATTGGCCA+AGG - chr4.2:38859582-38859601 None:intergenic 45.0%
TGGATATGCAAATGGCGCAA+TGG + chr4.2:38859094-38859113 MS.gene28357:CDS 45.0%
TTGAATCCAAATTGCACCGG+TGG + chr4.2:38859140-38859159 MS.gene28357:CDS 45.0%
TTTATGGATCCCTTGGCCAA+TGG + chr4.2:38859569-38859588 MS.gene28357:CDS 45.0%
! ACCAATGCTCGCATGGTTTT+TGG - chr4.2:38859602-38859621 None:intergenic 45.0%
! AGTGGCAGATTGTTTTCCTC+AGG - chr4.2:38860994-38861013 None:intergenic 45.0%
! CAGGGCTGGTGTAAATGTAA+AGG + chr4.2:38860403-38860422 MS.gene28357:intron 45.0%
! CGATGCATCATTATCGGTTG+AGG + chr4.2:38860220-38860239 MS.gene28357:CDS 45.0%
! TATGAAGGTGCAGCAAGTGA+AGG + chr4.2:38858338-38858357 MS.gene28357:CDS 45.0%
!! GTAGGCTAACAAGCTCTAGA+TGG - chr4.2:38859428-38859447 None:intergenic 45.0%
AACGCTGCACCACTGCAAAT+AGG + chr4.2:38858251-38858270 MS.gene28357:CDS 50.0%
AGACCCAAGCAAGGCTTACT+TGG - chr4.2:38861333-38861352 None:intergenic 50.0%
AGGCCAAGTAAGCCTTGCTT+GGG + chr4.2:38861327-38861346 MS.gene28357:CDS 50.0%
CACCAGGTCGATGTTCTTCT+TGG + chr4.2:38859117-38859136 MS.gene28357:CDS 50.0%
CTGAGGGAAGGGACTATGAA+TGG + chr4.2:38861159-38861178 MS.gene28357:CDS 50.0%
CTTCAATGAGGCTGAGGGAA+GGG + chr4.2:38861148-38861167 MS.gene28357:CDS 50.0%
GAATCACCACCGGTGCAATT+TGG - chr4.2:38859149-38859168 None:intergenic 50.0%
GTGGCAACACATCTCCTCAA+AGG - chr4.2:38861071-38861090 None:intergenic 50.0%
TTCGGTTCCAGAATCACCAC+CGG - chr4.2:38859159-38859178 None:intergenic 50.0%
! AATTGCACCGGTGGTGATTC+TGG + chr4.2:38859149-38859168 MS.gene28357:CDS 50.0%
! AGTGACACAGTCGGAAGTGT+AGG - chr4.2:38859446-38859465 None:intergenic 50.0%
! TGGAAGCATAGACCCAAGCA+AGG - chr4.2:38861342-38861361 None:intergenic 50.0%
CCTTCAATGAGGCTGAGGGA+AGG + chr4.2:38861147-38861166 MS.gene28357:CDS 55.0%
CCTTCCCTCAGCCTCATTGA+AGG - chr4.2:38861150-38861169 None:intergenic 55.0%
GAGGCCAAGTAAGCCTTGCT+TGG + chr4.2:38861326-38861345 MS.gene28357:CDS 55.0%
GCAGCAAGTGAAGGTGGAAG+AGG + chr4.2:38858347-38858366 MS.gene28357:CDS 55.0%
GTGATTGCAGTCGCACGAGA+TGG + chr4.2:38859874-38859893 MS.gene28357:intron 55.0%
! GAAGGTGCAGCAAGTGAAGG+TGG + chr4.2:38858341-38858360 MS.gene28357:CDS 55.0%
GCAAATGGCGCAATGGCACC+AGG + chr4.2:38859101-38859120 MS.gene28357:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 38858158 38861452 38858158 ID=MS.gene28357
chr4.2 mRNA 38858158 38861452 38858158 ID=MS.gene28357.t1;Parent=MS.gene28357
chr4.2 exon 38858158 38858542 38858158 ID=MS.gene28357.t1.exon1;Parent=MS.gene28357.t1
chr4.2 CDS 38858158 38858542 38858158 ID=cds.MS.gene28357.t1;Parent=MS.gene28357.t1
chr4.2 exon 38858636 38858713 38858636 ID=MS.gene28357.t1.exon2;Parent=MS.gene28357.t1
chr4.2 CDS 38858636 38858713 38858636 ID=cds.MS.gene28357.t1;Parent=MS.gene28357.t1
chr4.2 exon 38858814 38858901 38858814 ID=MS.gene28357.t1.exon3;Parent=MS.gene28357.t1
chr4.2 CDS 38858814 38858901 38858814 ID=cds.MS.gene28357.t1;Parent=MS.gene28357.t1
chr4.2 exon 38858995 38859366 38858995 ID=MS.gene28357.t1.exon4;Parent=MS.gene28357.t1
chr4.2 CDS 38858995 38859366 38858995 ID=cds.MS.gene28357.t1;Parent=MS.gene28357.t1
chr4.2 exon 38859568 38859785 38859568 ID=MS.gene28357.t1.exon5;Parent=MS.gene28357.t1
chr4.2 CDS 38859568 38859785 38859568 ID=cds.MS.gene28357.t1;Parent=MS.gene28357.t1
chr4.2 exon 38859884 38859915 38859884 ID=MS.gene28357.t1.exon6;Parent=MS.gene28357.t1
chr4.2 CDS 38859884 38859915 38859884 ID=cds.MS.gene28357.t1;Parent=MS.gene28357.t1
chr4.2 exon 38860015 38860117 38860015 ID=MS.gene28357.t1.exon7;Parent=MS.gene28357.t1
chr4.2 CDS 38860015 38860117 38860015 ID=cds.MS.gene28357.t1;Parent=MS.gene28357.t1
chr4.2 exon 38860204 38860312 38860204 ID=MS.gene28357.t1.exon8;Parent=MS.gene28357.t1
chr4.2 CDS 38860204 38860312 38860204 ID=cds.MS.gene28357.t1;Parent=MS.gene28357.t1
chr4.2 exon 38860406 38860515 38860406 ID=MS.gene28357.t1.exon9;Parent=MS.gene28357.t1
chr4.2 CDS 38860406 38860515 38860406 ID=cds.MS.gene28357.t1;Parent=MS.gene28357.t1
chr4.2 exon 38861076 38861452 38861076 ID=MS.gene28357.t1.exon10;Parent=MS.gene28357.t1
chr4.2 CDS 38861076 38861452 38861076 ID=cds.MS.gene28357.t1;Parent=MS.gene28357.t1
Gene Sequence

>MS.gene28357

ATGACATTAAGCATGAGATATCTCCTATTTTCTCTATTGTTGTCTTTAGCTTCAACATTCATTATTACTTTAACAGAAGGTCAAGAAATAATTAACGCTGCACCACTGCAAATAGGTTCATTGAATAGGAATGATTTTCCAGAAGGCTTCATATTTGGCACAGCATCTTCTGCATATCAATATGAAGGTGCAGCAAGTGAAGGTGGAAGAGGACCAAGTATATGGGATACTTTCACTCATAGATATCCTCAAAAGATCAGTGATGGAAACAATGGAGACGTTGCGGTTGACTCGTATCATCGATATAAGGAAGATGTTGGAATCATGAAGGATATGAACTTGGATGCATATAGATTTTCCATATCTTGGTCTAGAATACTCCCAAGTAAGTCAATTTTATACCAATTGACTATTCTCAATTAAACTTAGTTTCTTTTCACCAACCATATTTTTTATTTTTTTTTTATAATTTATGCAGAAGGAAAACTAAGTGGTGGAATTAATCAAGAAGGAATTGACTATTACAATAACCTCATTGATGAATTGGTGGCAAATGGTAAGAATACAAACTTCATTTTCAATTATTGTAGTATTATTATTTTTATTTGCATTTTAGCATTTTCCAAAATAGCCGTCTTATGTAAAAAAAATTGCAGGTTTACAACCATTTGTAACTCTTTTTCATTGGGATCTTCCTCAATCTCTAGAAGATGAATATGGCGGTTTCTTAAGTCCTCTCATCATGTGAGTGATACAATTTCAAATTTTAATCTGAAAATTTATTTTATATAGTTATGAAAATGTTTCTTATTCATCTTTTCATACCTCCAACTACAGAAAAGACTTTCAAAACTACGCGGAACTTTGCTTTAAAACATTTGGAGATAGAGTAAAACATTGGATAACTTTGAACGAGCCATGGAGTTACAGTCAAAATGGATATGCAAATGGCGCAATGGCACCAGGTCGATGTTCTTCTTGGTTGAATCCAAATTGCACCGGTGGTGATTCTGGAACCGAACCTTATTTAACTGCACACTATCAACTTCTAGCACATGCAGCAGCTGTTAATGTCTACAAGACCAAGTATCAAGTTTCTCAAAATGGTTTGATCGGTATAACATTGATCATTAACTGGAATGTGCCGCTCTCAGATAACAAACATAATCAAAATGCTGCGGAACGAGCTACAGACTTTCAGTTTGGATGGTAAGTACAAAGATAAAGCTATTTACACAAGTTTGTCGCATCCAAACTTGTTTCTTTTCCATCTAGAGCTTGTTAGCCTACACTTCCGACTGTGTCACTTACATAATTCTACCATCCAAATTAACACAAGCATATCTATCTATCTATCTCTCTCTATATATATACACATGATAACTTATTCCATTTCTTGTTTAATTATAGGTTTATGGATCCCTTGGCCAATGGAGATTATCCAAAAACCATGCGAGCATTGGTAAGATCACGATTACCAAAGTTTACTAAAGAGCAATCTAAGCAAGTCAAAGGTTCATTTGATTTTATTGGCATAAACTACTACTCTTCTTGCTATGTTTCTGATGCACCTCAATTAAGCAATGCAAATTCCAGCTACCTAACAGATTCTCTGTCTAGTTTTTCATGTAAGCACTTTTAGCTTACTGCTATCTTGTATTTATTGATTGTTTAATTAGTATTATAAATTTAAATGATGGTATATTTTATATTTTTGTGATTGCAGTCGCACGAGATGGAAAACCAATTGGTCTAAATGTAAGTTTATACGTTAAAAATTGCAACATCTTACTTTTTTTTTTTTTGGTTTTCTGTTATTGTGTAATTAATAAACTTGTTATGTGTATTTAATTTCAGGTTGCTTCAGAATGGTTGTATGTTTATCCAAGAGGAATTCGAGACTTTTTGATATACGCTAAAGAAAAATACAACAATCCTTTAATTTATATTACTGAAAACGGTACGCAAATCGTGCATGTATCATATTGAAACATATTTTCGTTATATAACGAAACTATGTATGATAATTCAAATTTTCATGTGTAGGTATAAATGAGTACGACGATGCATCATTATCGGTTGAGGAATCTCTTTTAGATATTTATAGAATCGATTATCATTACCGTCATCTCTTTTATCTTAAAGAGGCAATCATGTAAGTAATATATTGAAATATCTGAAGAGTAGTCTATTTCATTTTTTGCATGGAATTTAACTATATGATATTATTTCTTTCAATGAAATGCAGGGCTGGTGTAAATGTAAAGGGATACTTTGCATGGTCTCTGTTGGATAATTTTGAATGGCACAGGGGTTATACTGTGAGATTTGGAATGATTTTCATAGATTACAAAGATGGTTTGAAAAGATATCAAAAGCTCTCAGGATTGTGGTTCAAGAATTTTCTCACATCAGAGAACAAAAAAGTTTACATAGATTCCATTTAACAGTGCTACAAAATTCTTTATAAACACCATAGTTGTTGTTGTAAACACAATAATTTCATTTAATTATTTCATTGTTTAAATATAAGAAGGATACTATTTGTAATTGTATGTAAATATATTATTATAATTGATATTATTTGATGATTCTACATATATCTTGTAATTTCATTTTGCATGACTAGTCCCTTCACAAACTGAAGAATTTTTCCATTTTAACCAAGTTGTGATTACAAAAAATCTACTAGGGTATGTTTATGTCAAACATGAATATACCATTAGGCATCTCAAAATATGGTTTATACCTTTTAAGGAAAAAATAATTCCTAAAGTATATTATTCTAAATTGAAAATATTCTAGTTCTCTTGAAACGACTTACAACCTAAAACCTGAGGAAAACAATCTGCCACTATTAAACTGAATATAAGTTCGACAGATGAGGGATTGACTCTTAATCTCGAATAACCTTTGAGGAGATGTGTTGCCACATGTGATAAGGCGTATAGGAACAACTGCTACCAACATGTGTCTTCAAGATCCTATTCCTTCAATGAGGCTGAGGGAAGGGACTATGAATGGTGCAGTTCAGAATGTTGCAAATGCTGGTAAATCAAATGTTGGGTCCTCCATAATGGTTAATATGGATGGTCGTATCAATGCGGTGCACCATGAAATTAGTACTATCAAAACAGGTATCCTTGTTGCTCAACTAAGCATTCTCACGAGGCCAAGTAAGCCTTGCTTGGGTCTATGCTTCCATATGCTACTACTCCTATGCTTACAAATGGTGTGCAATAGCCATGTCAAGTTGTTCTTGATGTTGAAACAGATGCCTTGGGGATTGAACTGA
Protein sequence

>MS.gene28357.t1

MTLSMRYLLFSLLLSLASTFIITLTEGQEIINAAPLQIGSLNRNDFPEGFIFGTASSAYQYEGAASEGGRGPSIWDTFTHRYPQKISDGNNGDVAVDSYHRYKEDVGIMKDMNLDAYRFSISWSRILPKGKLSGGINQEGIDYYNNLIDELVANGLQPFVTLFHWDLPQSLEDEYGGFLSPLIIKDFQNYAELCFKTFGDRVKHWITLNEPWSYSQNGYANGAMAPGRCSSWLNPNCTGGDSGTEPYLTAHYQLLAHAAAVNVYKTKYQVSQNGLIGITLIINWNVPLSDNKHNQNAAERATDFQFGWFMDPLANGDYPKTMRALVRSRLPKFTKEQSKQVKGSFDFIGINYYSSCYVSDAPQLSNANSSYLTDSLSSFSFARDGKPIGLNVASEWLYVYPRGIRDFLIYAKEKYNNPLIYITENGINEYDDASLSVEESLLDIYRIDYHYRHLFYLKEAIMAGVNVKGYFAWSLLDNFEWHRGYTVRFGMIFIDYKDGDVLPHVIRRIGTTATNMCLQDPIPSMRLREGTMNGAVQNVANAGKSNVGSSIMVNMDGRINAVHHEISTIKTGILVAQLSILTRPSKPCLGLCFHMLLLLCLQMVCNSHVKLFLMLKQMPWGLN