AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene28358

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene28358.t1 MTR_4g066590 93.117 523 19 3 1 510 1 519 0.0 995
MS.gene28358.t1 MTR_4g066630 86.042 523 57 3 1 510 5 524 0.0 905
MS.gene28358.t1 MTR_4g066580 73.398 515 120 3 6 507 12 522 0.0 805
MS.gene28358.t1 MTR_4g066630 86.433 457 47 2 1 444 5 459 0.0 793
MS.gene28358.t1 MTR_3g028720 73.239 497 119 2 25 507 25 521 0.0 784
MS.gene28358.t1 MTR_4g067040 92.439 410 15 2 1 397 1 407 0.0 776
MS.gene28358.t1 MTR_4g067040 91.774 389 16 2 1 376 1 386 0.0 731
MS.gene28358.t1 MTR_4g066630 86.493 422 42 2 1 409 5 424 0.0 730
MS.gene28358.t1 MTR_2g098540 68.836 507 137 3 5 498 7 505 0.0 727
MS.gene28358.t1 MTR_2g098790 66.732 511 154 3 1 498 5 512 0.0 725
MS.gene28358.t1 MTR_2g098520 69.792 480 132 1 32 498 29 508 0.0 717
MS.gene28358.t1 MTR_6g078450 66.864 507 152 3 7 498 5 510 0.0 710
MS.gene28358.t1 MTR_4g066210 66.388 479 148 1 33 498 39 517 0.0 694
MS.gene28358.t1 MTR_3g028720 73.793 435 100 2 25 445 25 459 0.0 689
MS.gene28358.t1 MTR_2g098540 71.053 456 119 1 56 498 6 461 0.0 689
MS.gene28358.t1 MTR_6g078330 65.098 510 160 3 12 505 9 516 0.0 686
MS.gene28358.t1 MTR_4g066640 63.654 509 166 4 7 501 11 514 0.0 686
MS.gene28358.t1 MTR_4g066240 62.818 511 176 2 1 498 1 510 0.0 682
MS.gene28358.t1 MTR_4g066240 62.695 512 176 3 1 498 1 511 0.0 678
MS.gene28358.t1 MTR_6g078380 65.483 507 155 3 6 498 11 511 0.0 674
MS.gene28358.t1 MTR_5g071520 62.863 517 169 4 4 498 7 522 0.0 661
MS.gene28358.t1 MTR_4g066580 73.397 421 95 3 6 413 12 428 0.0 651
MS.gene28358.t1 MTR_4g066630 86.436 376 36 2 1 363 5 378 0.0 649
MS.gene28358.t1 MTR_3g028720 71.158 423 106 3 3 411 5 425 0.0 630
MS.gene28358.t1 MTR_6g078450 66.520 454 136 3 7 445 5 457 0.0 628
MS.gene28358.t1 MTR_4g066330 60.039 513 161 5 2 498 7 491 0.0 621
MS.gene28358.t1 MTR_3g026280 60.924 476 168 3 36 498 21 491 0.0 610
MS.gene28358.t1 MTR_4g066280 59.681 501 159 4 13 498 19 491 0.0 609
MS.gene28358.t1 MTR_4g066340 65.741 432 135 1 80 498 6 437 0.0 607
MS.gene28358.t1 MTR_4g023000 59.454 476 174 4 36 498 23 492 0.0 588
MS.gene28358.t1 MTR_6g078330 66.425 414 124 2 105 505 1 412 0.0 576
MS.gene28358.t1 MTR_3g028720 70.543 387 98 3 3 375 5 389 0.0 572
MS.gene28358.t1 MTR_4g066240 61.611 422 148 2 1 409 1 421 0.0 551
MS.gene28358.t1 MTR_6g078330 64.179 402 127 3 117 505 15 412 0.0 538
MS.gene28358.t1 MTR_2g043200 57.773 476 154 7 42 498 70 517 0.0 536
MS.gene28358.t1 MTR_4g015420 54.183 502 177 8 22 498 50 523 0.0 535
MS.gene28358.t1 MTR_3g026400 52.569 506 220 6 7 498 8 507 0.0 534
MS.gene28358.t1 MTR_3g026620 50.489 511 234 5 1 498 4 508 0.0 528
MS.gene28358.t1 MTR_4g015460 55.670 485 165 9 37 498 42 499 2.94e-180 516
MS.gene28358.t1 MTR_4g083000 50.791 506 227 6 10 498 1 501 2.02e-179 513
MS.gene28358.t1 MTR_4g066640 63.281 384 122 4 7 376 11 389 2.47e-178 506
MS.gene28358.t1 MTR_6g078330 69.851 335 88 1 37 358 37 371 3.19e-176 501
MS.gene28358.t1 MTR_5g044000 49.809 524 221 8 13 498 1 520 5.33e-176 505
MS.gene28358.t1 MTR_3g026400 53.533 467 196 6 46 498 17 476 2.44e-175 502
MS.gene28358.t1 MTR_4g081530 49.509 509 233 7 13 505 5 505 2.13e-165 478
MS.gene28358.t1 MTR_0110s0040 47.266 512 244 8 3 500 13 512 2.00e-161 468
MS.gene28358.t1 MTR_0110s0030 46.680 512 247 8 3 500 13 512 1.90e-160 466
MS.gene28358.t1 MTR_3g033790 47.193 481 227 6 37 504 19 485 4.61e-150 438
MS.gene28358.t1 MTR_3g033810 46.830 489 233 6 30 504 14 489 2.23e-149 437
MS.gene28358.t1 MTR_4g131810 46.362 481 228 6 42 500 33 505 2.04e-145 427
MS.gene28358.t1 MTR_2g022760 47.059 476 234 5 46 506 45 517 3.39e-144 424
MS.gene28358.t1 MTR_4g083000 51.095 411 180 5 105 498 1 407 9.47e-142 414
MS.gene28358.t1 MTR_2g079550 44.581 489 243 9 37 505 30 510 5.70e-139 411
MS.gene28358.t1 MTR_2g079580 41.794 524 269 11 2 505 5 512 1.26e-138 410
MS.gene28358.t1 MTR_4g131800 46.610 472 233 6 42 498 521 988 1.80e-137 422
MS.gene28358.t1 MTR_4g016920 45.625 480 232 8 36 500 26 491 4.77e-137 406
MS.gene28358.t1 MTR_7g068910 44.561 478 236 7 36 498 30 493 1.75e-136 404
MS.gene28358.t1 MTR_3g026400 54.902 357 143 5 7 349 8 360 1.58e-134 394
MS.gene28358.t1 MTR_7g033860 43.595 484 233 8 39 501 50 514 6.30e-134 398
MS.gene28358.t1 MTR_7g068910 43.648 488 236 8 36 498 30 503 2.25e-133 397
MS.gene28358.t1 MTR_2g022710 41.879 511 275 8 4 499 5 508 1.46e-132 395
MS.gene28358.t1 MTR_1g088610 43.615 509 239 11 10 498 7 487 5.16e-129 389
MS.gene28358.t1 MTR_1g088610 36.207 116 67 2 384 498 503 612 1.65e-12 70.5
MS.gene28358.t1 MTR_5g068740 43.137 510 241 11 10 498 7 488 5.69e-129 385
MS.gene28358.t1 MTR_0108s0030 42.029 483 230 6 39 504 5 454 2.82e-128 381
MS.gene28358.t1 MTR_7g051610 42.678 478 241 7 38 501 76 534 6.23e-120 363
MS.gene28358.t1 MTR_0110s0040 47.089 395 186 6 3 382 13 399 2.81e-119 357
MS.gene28358.t1 MTR_7g051510 41.079 482 249 6 38 504 76 537 3.61e-117 356
MS.gene28358.t1 MTR_7g051650 41.458 480 247 7 38 502 76 536 1.93e-116 354
MS.gene28358.t1 MTR_7g051650 41.042 480 245 7 38 502 76 532 4.17e-115 350
MS.gene28358.t1 MTR_2g022760 44.604 417 213 5 105 506 1 414 1.93e-114 345
MS.gene28358.t1 MTR_2g079580 45.013 391 190 7 37 409 32 415 2.85e-113 342
MS.gene28358.t1 MTR_7g068900 44.524 420 194 9 100 498 17 418 2.78e-112 342
MS.gene28358.t1 MTR_2g022710 40.700 457 250 7 4 445 5 455 2.88e-109 333
MS.gene28358.t1 MTR_4g016920 44.282 411 201 7 105 500 1 398 3.69e-109 331
MS.gene28358.t1 MTR_1g088630 42.981 463 199 12 56 498 14 431 4.03e-109 332
MS.gene28358.t1 MTR_2g022710 40.700 457 250 7 4 445 5 455 7.19e-109 332
MS.gene28358.t1 MTR_2g022710 41.805 421 224 7 4 409 5 419 4.05e-106 323
MS.gene28358.t1 MTR_2g022710 40.732 410 225 5 105 499 1 407 8.08e-104 317
MS.gene28358.t1 MTR_2g022710 41.076 409 220 7 4 397 5 407 3.91e-100 308
MS.gene28358.t1 MTR_7g056747 41.866 418 204 5 99 502 9 401 1.41e-99 306
MS.gene28358.t1 MTR_1g088630 48.758 322 116 7 39 343 29 318 1.48e-95 294
MS.gene28358.t1 MTR_2g022710 42.188 384 201 7 4 372 5 382 6.80e-94 292
MS.gene28358.t1 MTR_4g016920 51.685 267 113 3 105 357 1 265 2.12e-87 271
MS.gene28358.t1 MTR_0110s0020 50.588 255 118 4 3 257 13 259 1.13e-86 270
MS.gene28358.t1 MTR_1g088630 51.301 269 106 5 39 290 29 289 1.45e-85 266
MS.gene28358.t1 MTR_5g069350 43.860 171 55 5 338 498 48 187 9.55e-31 118
MS.gene28358.t1 MTR_4g122980 26.214 412 232 12 101 500 183 534 1.18e-27 117
MS.gene28358.t1 MTR_8g038650 44.898 98 51 1 248 345 58 152 1.66e-22 94.4
MS.gene28358.t1 MTR_8g038650 55.556 54 23 1 46 99 7 59 2.11e-13 68.6
MS.gene28358.t1 MTR_4g061170 80.488 41 6 1 447 485 24 64 4.33e-15 70.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene28358.t1 AT5G44640 52.259 509 222 6 9 503 5 506 0.0 546
MS.gene28358.t1 AT5G42260 53.293 501 213 6 8 494 4 497 0.0 543
MS.gene28358.t1 AT2G44450 53.183 487 210 3 25 498 19 500 0.0 541
MS.gene28358.t1 AT2G44480 52.917 480 212 2 36 502 65 543 0.0 540
MS.gene28358.t1 AT2G44480 52.917 480 212 2 36 502 34 512 0.0 540
MS.gene28358.t1 AT3G60130 53.782 476 201 4 37 498 30 500 0.0 530
MS.gene28358.t1 AT5G24550 50.192 520 239 7 8 508 1 519 0.0 523
MS.gene28358.t1 AT5G24540 50.577 520 237 7 8 508 1 519 5.40e-180 516
MS.gene28358.t1 AT2G25630 50.489 511 208 6 2 498 4 483 2.40e-179 513
MS.gene28358.t1 AT3G60140 49.702 503 229 8 12 498 8 502 3.55e-178 513
MS.gene28358.t1 AT2G44460 46.641 521 252 6 1 506 3 512 5.68e-172 498
MS.gene28358.t1 AT3G60120 51.559 481 211 7 36 498 13 489 5.94e-172 496
MS.gene28358.t1 AT1G26560 51.660 482 210 7 37 500 32 508 9.11e-172 494
MS.gene28358.t1 AT1G51470 52.401 479 201 8 36 499 44 510 1.17e-171 494
MS.gene28358.t1 AT1G47600 51.775 479 204 8 36 499 44 510 4.52e-169 488
MS.gene28358.t1 AT1G47600 51.775 479 203 8 36 499 44 509 9.89e-169 486
MS.gene28358.t1 AT2G44490 49.691 485 227 5 36 503 13 497 7.74e-168 486
MS.gene28358.t1 AT2G44470 45.437 515 254 6 1 499 3 506 2.09e-167 486
MS.gene28358.t1 AT5G54570 49.278 485 216 5 36 498 29 505 5.15e-166 481
MS.gene28358.t1 AT3G18080 48.902 501 231 9 20 503 20 512 9.15e-165 476
MS.gene28358.t1 AT5G25980 47.280 533 250 11 1 510 12 536 1.20e-159 465
MS.gene28358.t1 AT3G18070 48.566 488 218 10 32 503 25 495 1.73e-158 460
MS.gene28358.t1 AT3G18070 48.770 488 223 10 32 503 25 501 1.25e-157 458
MS.gene28358.t1 AT3G09260 47.917 480 232 6 37 500 35 512 1.26e-155 454
MS.gene28358.t1 AT2G44480 50.852 411 188 2 105 502 1 410 3.87e-155 448
MS.gene28358.t1 AT3G60130 52.174 414 179 4 99 498 40 448 6.52e-153 444
MS.gene28358.t1 AT3G03640 44.814 511 259 6 7 498 6 512 6.74e-152 444
MS.gene28358.t1 AT1G75940 47.143 490 232 9 37 503 37 522 3.87e-151 442
MS.gene28358.t1 AT2G32860 44.699 481 249 4 36 500 93 572 4.33e-151 445
MS.gene28358.t1 AT2G32860 44.699 481 250 4 36 500 93 573 5.48e-151 445
MS.gene28358.t1 AT5G24540 49.451 455 211 6 8 444 1 454 1.79e-150 438
MS.gene28358.t1 AT5G36890 48.125 480 223 5 38 503 17 484 1.83e-150 439
MS.gene28358.t1 AT5G36890 48.125 480 223 5 38 503 17 484 1.90e-150 439
MS.gene28358.t1 AT1G52400 45.312 512 256 10 6 498 9 515 3.18e-149 437
MS.gene28358.t1 AT1G52400 45.312 512 256 10 6 498 9 515 3.18e-149 437
MS.gene28358.t1 AT3G21370 46.058 482 239 8 36 498 32 511 1.83e-148 436
MS.gene28358.t1 AT5G28510 46.296 486 238 7 36 500 38 521 2.31e-148 436
MS.gene28358.t1 AT1G66280 45.908 501 250 9 16 498 13 510 6.02e-147 432
MS.gene28358.t1 AT5G26000 45.455 517 249 10 7 498 3 511 6.11e-147 432
MS.gene28358.t1 AT3G60130 51.208 414 172 5 99 498 40 437 1.12e-145 426
MS.gene28358.t1 AT1G66270 46.250 480 239 8 37 500 36 512 1.27e-144 426
MS.gene28358.t1 AT2G44470 44.009 459 231 5 1 444 3 450 5.30e-144 421
MS.gene28358.t1 AT5G54570 48.260 431 194 4 36 445 29 451 5.60e-143 419
MS.gene28358.t1 AT1G66270 46.042 480 238 9 37 500 36 510 1.27e-141 418
MS.gene28358.t1 AT3G60140 48.668 413 189 7 12 409 8 412 1.36e-141 416
MS.gene28358.t1 AT1G02850 46.526 475 224 8 38 498 27 485 2.47e-139 411
MS.gene28358.t1 AT5G25980 46.983 464 219 8 1 445 12 467 7.38e-139 409
MS.gene28358.t1 AT5G25980 46.983 464 219 8 1 445 12 467 7.38e-139 409
MS.gene28358.t1 AT1G61820 45.306 490 247 8 29 501 22 507 8.33e-139 410
MS.gene28358.t1 AT1G02850 46.753 462 215 8 38 498 27 458 5.30e-136 402
MS.gene28358.t1 AT1G02850 44.378 498 224 8 38 498 27 508 6.58e-136 403
MS.gene28358.t1 AT1G02850 44.489 499 223 9 38 498 27 509 2.45e-134 399
MS.gene28358.t1 AT5G54570 47.837 416 187 5 105 498 1 408 1.13e-132 392
MS.gene28358.t1 AT1G60090 43.061 490 240 11 37 509 24 491 1.78e-131 391
MS.gene28358.t1 AT4G21760 41.487 511 271 9 6 498 21 521 8.40e-130 389
MS.gene28358.t1 AT2G44480 57.692 312 117 3 36 334 65 374 2.31e-128 379
MS.gene28358.t1 AT4G27830 41.304 506 253 10 11 498 5 484 5.95e-128 382
MS.gene28358.t1 AT1G60090 41.800 500 239 12 37 509 24 498 1.89e-127 381
MS.gene28358.t1 AT5G26000 44.372 462 226 8 7 445 3 456 4.54e-127 378
MS.gene28358.t1 AT1G61810 43.416 486 253 9 42 509 38 519 1.93e-126 379
MS.gene28358.t1 AT4G22100 42.946 482 240 10 38 502 23 486 1.50e-125 376
MS.gene28358.t1 AT4G27820 42.292 480 241 8 36 498 22 482 3.93e-125 375
MS.gene28358.t1 AT1G52400 43.982 457 233 9 6 444 9 460 3.21e-124 371
MS.gene28358.t1 AT2G44450 50.000 354 159 3 158 498 1 349 1.05e-123 366
MS.gene28358.t1 AT2G44470 43.457 405 203 5 1 390 3 396 1.19e-123 367
MS.gene28358.t1 AT4G21760 40.509 511 266 10 6 498 21 511 9.36e-123 370
MS.gene28358.t1 AT1G61810 44.252 461 236 8 42 485 38 494 6.85e-122 368
MS.gene28358.t1 AT1G45191 42.678 478 233 10 38 498 30 483 1.22e-121 366
MS.gene28358.t1 AT3G62750 40.408 490 247 9 26 498 12 473 5.68e-120 362
MS.gene28358.t1 AT3G62750 40.871 482 240 9 34 498 46 499 1.18e-119 362
MS.gene28358.t1 AT1G45191 41.273 487 238 10 38 498 30 494 4.39e-118 357
MS.gene28358.t1 AT1G45191 41.423 478 245 10 38 498 30 489 1.18e-117 356
MS.gene28358.t1 AT1G45191 40.845 497 238 11 38 498 30 506 1.10e-116 354
MS.gene28358.t1 AT1G02850 45.588 408 176 6 38 409 27 424 1.21e-115 348
MS.gene28358.t1 AT3G62740 39.709 481 252 11 34 498 20 478 3.99e-114 347
MS.gene28358.t1 AT5G48375 44.519 447 163 10 33 463 34 411 3.76e-113 342
MS.gene28358.t1 AT3G18070 50.606 330 143 6 32 348 25 347 7.32e-111 334
MS.gene28358.t1 AT2G44460 45.628 366 184 4 156 506 1 366 7.90e-110 333
MS.gene28358.t1 AT1G60270 47.384 344 157 6 37 364 24 359 3.52e-106 322
MS.gene28358.t1 AT3G62750 39.726 438 220 8 26 447 38 447 2.20e-102 315
MS.gene28358.t1 AT1G51490 47.159 352 156 8 44 379 27 364 1.15e-100 309
MS.gene28358.t1 AT1G45191 47.024 336 153 6 38 357 30 356 5.78e-100 307
MS.gene28358.t1 AT1G45191 47.024 336 153 6 38 357 30 356 6.46e-100 307
MS.gene28358.t1 AT3G62740 39.906 426 219 10 34 444 20 423 1.33e-99 306
MS.gene28358.t1 AT1G61810 41.135 423 227 9 105 509 1 419 3.44e-99 305
MS.gene28358.t1 AT4G27830 40.244 410 215 6 105 498 1 396 1.64e-98 304
MS.gene28358.t1 AT1G61810 43.377 385 198 7 42 409 38 419 1.68e-98 304
MS.gene28358.t1 AT4G27820 40.633 411 213 7 105 498 1 397 1.97e-96 298
MS.gene28358.t1 AT4G27820 40.633 411 213 7 105 498 1 397 1.97e-96 298
MS.gene28358.t1 AT3G62750 44.615 325 151 5 34 345 20 328 9.36e-95 292
MS.gene28358.t1 AT3G62750 44.615 325 151 5 34 345 20 328 9.36e-95 292
MS.gene28358.t1 AT3G18070 46.084 332 157 7 188 503 99 424 6.14e-94 292
MS.gene28358.t1 AT3G18070 58.824 68 27 1 32 99 25 91 4.13e-18 87.0
MS.gene28358.t1 AT3G62750 43.844 333 158 5 26 345 38 354 7.09e-94 292
MS.gene28358.t1 AT2G44480 46.026 302 149 2 214 502 6 306 1.34e-93 287
MS.gene28358.t1 AT1G61820 42.663 368 191 7 151 501 4 368 3.09e-90 281
MS.gene28358.t1 AT3G62750 38.200 411 214 8 105 498 1 388 1.48e-88 278
MS.gene28358.t1 AT3G62750 38.200 411 214 8 105 498 1 388 1.48e-88 278
MS.gene28358.t1 AT3G18070 45.283 318 152 7 202 503 1 312 8.41e-87 270
MS.gene28358.t1 AT1G61810 48.679 265 121 3 42 293 38 300 7.27e-80 252
MS.gene28358.t1 AT1G60270 46.014 276 129 5 105 364 1 272 1.25e-76 243
MS.gene28358.t1 AT3G18070 43.096 239 119 4 188 411 99 335 1.38e-59 200
MS.gene28358.t1 AT3G18070 58.824 68 27 1 32 99 25 91 2.13e-18 87.0
MS.gene28358.t1 AT3G18070 41.778 225 114 4 202 411 1 223 6.68e-53 179
MS.gene28358.t1 AT5G16580 34.472 322 169 8 152 455 2 299 6.45e-42 152
MS.gene28358.t1 AT3G06510 26.478 423 253 14 100 500 159 545 2.34e-27 116
MS.gene28358.t1 AT3G06510 26.406 409 237 13 100 500 159 511 1.98e-26 113

Find 92 sgRNAs with CRISPR-Local

Find 163 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTATTTAATTATAGGTTTA+TGG 0.116590 4.2:+38873877 MS.gene28358:intron
TTGATTTATATTACTGAAAA+TGG 0.156629 4.2:+38874424 MS.gene28358:CDS
TTCCCAGAAGGCTTTATATT+TGG 0.203107 4.2:+38872502 MS.gene28358:CDS
GAATCACCGCCGGTGCAATT+TGG 0.245604 4.2:-38873270 None:intergenic
CACCAGGTCGATGCTCTTCT+TGG 0.247168 4.2:+38873241 MS.gene28358:CDS
AATTGCACCGGCGGTGATTC+TGG 0.263758 4.2:+38873273 MS.gene28358:CDS
CAACAACCTCATTGATGAAT+TGG 0.285597 4.2:+38872906 MS.gene28358:CDS
GTGGAAGAGGACCAAGTATA+TGG 0.292452 4.2:+38872569 MS.gene28358:CDS
GAACTTTGCTTCAAAACATT+TGG 0.292593 4.2:+38873141 MS.gene28358:CDS
ATCACTTGAGGAATCACTTA+TGG 0.300722 4.2:+38874611 MS.gene28358:CDS
ATTTCCAGGTTGCTTCAAAT+TGG 0.303894 4.2:+38874335 MS.gene28358:intron
TGATAATGTGCAACTAAATA+AGG 0.310787 4.2:-38873303 None:intergenic
GTGCCAAATATAAAGCCTTC+TGG 0.324054 4.2:-38872505 None:intergenic
AAGGGTTATACTGTCAGATT+TGG 0.344341 4.2:+38874848 MS.gene28358:CDS
AATGTTGTAGCACTGTTAAA+TGG 0.349883 4.2:-38874978 None:intergenic
AAACTGCAGAAAAGATTTCC+AGG 0.361097 4.2:+38873110 MS.gene28358:intron
CAAACTCTGGAAGATGAATA+TGG 0.365046 4.2:+38872979 MS.gene28358:CDS
AGATAACAAACTTGATATAA+AGG 0.370220 4.2:+38873434 MS.gene28358:CDS
AAGACAAGAAGAGAATAAAT+AGG 0.370429 4.2:-38872390 None:intergenic
AACACTGAATCACTGCAAAT+AGG 0.401235 4.2:+38872460 MS.gene28358:CDS
AAAAGATATCAAAAGCTCTC+AGG 0.405939 4.2:+38874902 MS.gene28358:CDS
AGAGCTACAGACTTCATGTT+TGG 0.408860 4.2:+38873465 MS.gene28358:CDS
TGACTCGTATCATCGCTATA+AGG 0.410464 4.2:+38872654 MS.gene28358:CDS
TGCCAAATATAAAGCCTTCT+GGG 0.411107 4.2:-38872504 None:intergenic
GTCTCTCAAAAGGGTGTGAT+AGG 0.412344 4.2:+38873375 MS.gene28358:intron
ATACTTTGCATGGTCTTTGT+TGG 0.413214 4.2:+38874808 MS.gene28358:CDS
TTGGGATCTTCCTCAAACTC+TGG 0.415319 4.2:+38872966 MS.gene28358:CDS
AGAAAGTCTCGAATTGCTCT+TGG 0.442143 4.2:-38874370 None:intergenic
TTATAGGTTTATGGATCCCT+TGG 0.446664 4.2:+38873886 MS.gene28358:intron
ATTGATGAATTGGTGGCAAA+TGG 0.451177 4.2:+38872916 MS.gene28358:CDS
TGTTCTTACCAAGGCTCTCA+TGG 0.452605 4.2:-38873930 None:intergenic
CAGGGCTGGTGTAAATGTAA+AGG 0.460153 4.2:+38874784 MS.gene28358:intron
TTTCAGTAATATAAATCAAA+GGG 0.466034 4.2:-38874420 None:intergenic
AGAGAATCTGTAAGGTAGCT+AGG 0.466863 4.2:-38874073 None:intergenic
TATTCATCTTCCAGAGTTTG+AGG 0.478589 4.2:-38872976 None:intergenic
AGGGCTGGTGTAAATGTAAA+GGG 0.484456 4.2:+38874785 MS.gene28358:intron
GCAGCAAGTGAAGGTGGAAG+AGG 0.487766 4.2:+38872556 MS.gene28358:CDS
AACACTTACATTTAGACCAA+TGG 0.493905 4.2:-38874231 None:intergenic
TCATTTATTCTATCTTAATG+AGG 0.494555 4.2:+38874662 MS.gene28358:CDS
TGGGTATGCAAATGGTGAAA+TGG 0.498834 4.2:+38873218 MS.gene28358:CDS
CCATGGACTTACAGTCAAGA+TGG 0.501018 4.2:+38873198 MS.gene28358:CDS
ATCAAAAGCTCTCAGGGTTG+TGG 0.504177 4.2:+38874909 MS.gene28358:CDS
AGTTTACAAAAGAGCAATCA+AGG 0.505341 4.2:+38873963 MS.gene28358:CDS
AGCAAAGTTCTGCATAGTCC+TGG 0.506435 4.2:-38873128 None:intergenic
AGTCAAGATGGGTATGCAAA+TGG 0.507686 4.2:+38873210 MS.gene28358:CDS
TGGATAATCTCCATTGGCCA+AGG 0.518626 4.2:-38873903 None:intergenic
CAACCTCATTGATGAATTGG+TGG 0.519451 4.2:+38872909 MS.gene28358:CDS
CATCGCTATAAGGAAGATGT+TGG 0.525320 4.2:+38872664 MS.gene28358:CDS
TTCATCATTACTTTAACAGA+AGG 0.528077 4.2:+38872424 MS.gene28358:CDS
TGGAAGAGGACCAAGTATAT+GGG 0.531156 4.2:+38872570 MS.gene28358:CDS
AACGAGAAAGAGAATCTGTA+AGG 0.533045 4.2:-38874081 None:intergenic
CTACAGACTTCATGTTTGGA+TGG 0.540670 4.2:+38873469 MS.gene28358:CDS
TTTATGGATCCCTTGGCCAA+TGG 0.549813 4.2:+38873893 MS.gene28358:CDS
AAAATCACTGATGGAAACAA+TGG 0.550597 4.2:+38872619 MS.gene28358:CDS
TTGGAGATAGAGTAAAACAT+TGG 0.559088 4.2:+38873160 MS.gene28358:CDS
TCTTCTGCATATCAGTATGA+AGG 0.560527 4.2:+38872532 MS.gene28358:CDS
GTGAAAGTATCCCATATACT+TGG 0.569026 4.2:-38872580 None:intergenic
GCAAATGGTGAAATGGCACC+AGG 0.576425 4.2:+38873225 MS.gene28358:CDS
CCATCTTGACTGTAAGTCCA+TGG 0.578921 4.2:-38873198 None:intergenic
TTGCCACCAATTCATCAATG+AGG 0.580311 4.2:-38872912 None:intergenic
TGGTTGAATCCAAATTGCAC+CGG 0.581033 4.2:+38873261 MS.gene28358:CDS
AAAGATATCAAAAGCTCTCA+GGG 0.581366 4.2:+38874903 MS.gene28358:CDS
GTATCACTCACATGATGAGT+GGG 0.583322 4.2:-38873014 None:intergenic
GGATAATCTCCATTGGCCAA+GGG 0.583402 4.2:-38873902 None:intergenic
TCAAGTTTGTTATCTGAGAG+TGG 0.587930 4.2:-38873426 None:intergenic
AATCATGAAGGATATGAACT+TGG 0.589040 4.2:+38872687 MS.gene28358:CDS
GGAAGATGTTGGAATCATGA+AGG 0.590169 4.2:+38872675 MS.gene28358:CDS
AACCAAGAAGAGCATCGACC+TGG 0.592148 4.2:-38873243 None:intergenic
GGATAACTTTGAATGAGCCA+TGG 0.594373 4.2:+38873181 MS.gene28358:CDS
TGATCCATCGTTATCACTTG+AGG 0.594621 4.2:+38874599 MS.gene28358:CDS
AGTGGAGGAATTAATCAAGA+AGG 0.596031 4.2:+38872871 MS.gene28358:CDS
GAAGGTGCAGCAAGTGAAGG+TGG 0.597164 4.2:+38872550 MS.gene28358:CDS
CATGGACTTACAGTCAAGAT+GGG 0.597238 4.2:+38873199 MS.gene28358:CDS
TATGCAGATGGAAAACTAAG+TGG 0.598010 4.2:+38872853 MS.gene28358:intron
TATGAAGGTGCAGCAAGTGA+AGG 0.604375 4.2:+38872547 MS.gene28358:CDS
AATAGAAAAGACTTCCCAGA+AGG 0.610702 4.2:+38872490 MS.gene28358:CDS
TGTATCACTCACATGATGAG+TGG 0.613126 4.2:-38873015 None:intergenic
TTCCGTTCCAGAATCACCGC+CGG 0.613932 4.2:-38873280 None:intergenic
CACCGGCGGTGATTCTGGAA+CGG 0.616221 4.2:+38873278 MS.gene28358:CDS
GTGATTGCAGTTGAACGTGA+TGG 0.617225 4.2:+38874203 MS.gene28358:intron
TGGTAACCTTGTTCTTACCA+AGG 0.618332 4.2:-38873939 None:intergenic
GGGTGTGATAGGTATAACAT+TGG 0.623641 4.2:+38873386 MS.gene28358:intron
ATGTAAAGGGATACTTTGCA+TGG 0.626161 4.2:+38874798 MS.gene28358:CDS
TTGAATCCAAATTGCACCGG+CGG 0.626765 4.2:+38873264 MS.gene28358:CDS
GGTTTGCCATTGCTCAACTG+AGG 0.628802 4.2:-38874052 None:intergenic
GATGCACCTCAGTTGAGCAA+TGG 0.634368 4.2:+38874046 MS.gene28358:CDS
GCAGATGGAAAACTAAGTGG+AGG 0.645764 4.2:+38872856 MS.gene28358:intron
GAGCAATCAAGGCTAGTGAG+TGG 0.679451 4.2:+38873974 MS.gene28358:CDS
GATTCCTCAAGTGATAACGA+TGG 0.682607 4.2:-38874603 None:intergenic
TGAGAGCCTTGGTAAGAACA+AGG 0.686788 4.2:+38873933 MS.gene28358:CDS
TGGAAACAATGGAGACGTTG+CGG 0.721837 4.2:+38872630 MS.gene28358:CDS
ACTCTGGAAGATGAATATGG+CGG 0.785553 4.2:+38872982 MS.gene28358:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GTTATCATATATATATATAT+AGG - chr4.2:38873845-38873864 None:intergenic 10.0%
!! TATCATATATATATATATAG+GGG - chr4.2:38873843-38873862 None:intergenic 10.0%
!! TTTATTTAATTATAGGTTTA+TGG + chr4.2:38873877-38873896 MS.gene28358:intron 10.0%
!!! CTCTTTTTTTATTTAATTAT+AGG + chr4.2:38873870-38873889 MS.gene28358:intron 10.0%
!! AAGGAGATATATATATATAT+GGG + chr4.2:38873530-38873549 MS.gene28358:intron 15.0%
!! AGATAAACATATAATTGATA+GGG - chr4.2:38872785-38872804 None:intergenic 15.0%
!! TAACTTAGTTTAAATATTTG+TGG + chr4.2:38873057-38873076 MS.gene28358:intron 15.0%
!! TTGATTTATATTACTGAAAA+TGG + chr4.2:38874424-38874443 MS.gene28358:CDS 15.0%
!! TTTCAGTAATATAAATCAAA+GGG - chr4.2:38874423-38874442 None:intergenic 15.0%
!!! AAATTGGTAATTAATGAATT+TGG + chr4.2:38874305-38874324 MS.gene28358:intron 15.0%
!!! TTTCTTTTAATGTAAATACA+GGG + chr4.2:38874766-38874785 MS.gene28358:intron 15.0%
!!! TTTTCAGTAATATAAATCAA+AGG - chr4.2:38874424-38874443 None:intergenic 15.0%
!! AGATAACAAACTTGATATAA+AGG + chr4.2:38873434-38873453 MS.gene28358:CDS 20.0%
!! AGGAGATATATATATATATG+GGG + chr4.2:38873531-38873550 MS.gene28358:intron 20.0%
!! ATCAATAGATCATATAAAGT+AGG - chr4.2:38874129-38874148 None:intergenic 20.0%
!! CAAGGAGATATATATATATA+TGG + chr4.2:38873529-38873548 MS.gene28358:intron 20.0%
!! GAGATAAACATATAATTGAT+AGG - chr4.2:38872786-38872805 None:intergenic 20.0%
!! TCATTTATTCTATCTTAATG+AGG + chr4.2:38874662-38874681 MS.gene28358:CDS 20.0%
!!! AATTTGGTTATTTTATTTCC+AGG + chr4.2:38874321-38874340 MS.gene28358:intron 20.0%
!!! ATTTGTAACACTTTTTCATT+GGG + chr4.2:38872948-38872967 MS.gene28358:CDS 20.0%
!!! CTTTCTTTTAATGTAAATAC+AGG + chr4.2:38874765-38874784 MS.gene28358:intron 20.0%
!!! TAATTGGTTTTTGTCTAAAT+TGG + chr4.2:38874289-38874308 MS.gene28358:intron 20.0%
! AAGACAAGAAGAGAATAAAT+AGG - chr4.2:38872393-38872412 None:intergenic 25.0%
! ACTTCATTAATTTATGCAGA+TGG + chr4.2:38872841-38872860 MS.gene28358:intron 25.0%
! GCAAGTAAATAACTAAAAAG+TGG - chr4.2:38873628-38873647 None:intergenic 25.0%
! TAATCATCCAAATTAACACA+AGG + chr4.2:38873511-38873530 MS.gene28358:intron 25.0%
! TATATCTCCTTGTGTTAATT+TGG - chr4.2:38873521-38873540 None:intergenic 25.0%
! TTCATCATTACTTTAACAGA+AGG + chr4.2:38872424-38872443 MS.gene28358:CDS 25.0%
!! CATACAGATTTTCTATATCT+TGG + chr4.2:38872713-38872732 MS.gene28358:CDS 25.0%
!! TGATAATGTGCAACTAAATA+AGG - chr4.2:38873306-38873325 None:intergenic 25.0%
!!! AACTTTTTCATAATGTGTGT+AGG + chr4.2:38874561-38874580 MS.gene28358:intron 25.0%
!!! AGTGGTTCATTTGATTTTAT+TGG + chr4.2:38873992-38874011 MS.gene28358:CDS 25.0%
!!! CAATGAAAAAGTGTTACAAA+TGG - chr4.2:38872949-38872968 None:intergenic 25.0%
!!! CATTTGTAACACTTTTTCAT+TGG + chr4.2:38872947-38872966 MS.gene28358:CDS 25.0%
AAAAGATATCAAAAGCTCTC+AGG + chr4.2:38874902-38874921 MS.gene28358:CDS 30.0%
AAAATCACTGATGGAAACAA+TGG + chr4.2:38872619-38872638 MS.gene28358:CDS 30.0%
AAAGATATCAAAAGCTCTCA+GGG + chr4.2:38874903-38874922 MS.gene28358:CDS 30.0%
AACACTTACATTTAGACCAA+TGG - chr4.2:38874234-38874253 None:intergenic 30.0%
AAGTATCAAGTCTCTCAAAA+GGG + chr4.2:38873366-38873385 MS.gene28358:intron 30.0%
AATCATGAAGGATATGAACT+TGG + chr4.2:38872687-38872706 MS.gene28358:CDS 30.0%
ACCTTCGTAGATTACAAAAA+TGG + chr4.2:38874875-38874894 MS.gene28358:CDS 30.0%
ACCTTCTAACAATAACAAGT+GGG - chr4.2:38873719-38873738 None:intergenic 30.0%
ACTCCCACATAAAAAATAGT+GGG - chr4.2:38873571-38873590 None:intergenic 30.0%
AGTTTACAAAAGAGCAATCA+AGG + chr4.2:38873963-38873982 MS.gene28358:CDS 30.0%
ATCATATAAAGTAGGCCAAA+AGG - chr4.2:38874121-38874140 None:intergenic 30.0%
GAACTTTGCTTCAAAACATT+TGG + chr4.2:38873141-38873160 MS.gene28358:CDS 30.0%
GCAATATCTCATCTGTTAAT+TGG + chr4.2:38874273-38874292 MS.gene28358:intron 30.0%
GTCTATTTCACTTTCTTTCA+TGG + chr4.2:38874723-38874742 MS.gene28358:intron 30.0%
TATCCTCAAAAAATCACTGA+TGG + chr4.2:38872610-38872629 MS.gene28358:CDS 30.0%
TCAATTGACTAGACATACTT+GGG - chr4.2:38872750-38872769 None:intergenic 30.0%
TCATATAAAGTAGGCCAAAA+GGG - chr4.2:38874120-38874139 None:intergenic 30.0%
TGAGATATTGCAACATTTCA+TGG - chr4.2:38874264-38874283 None:intergenic 30.0%
TTATAGCAACATACACCTTA+GGG + chr4.2:38873742-38873761 MS.gene28358:intron 30.0%
TTCAATTGACTAGACATACT+TGG - chr4.2:38872751-38872770 None:intergenic 30.0%
TTGGAGATAGAGTAAAACAT+TGG + chr4.2:38873160-38873179 MS.gene28358:CDS 30.0%
! AAAGTATCAAGTCTCTCAAA+AGG + chr4.2:38873365-38873384 MS.gene28358:intron 30.0%
! CTTTGTTGGACAATTTTGAA+TGG + chr4.2:38874822-38874841 MS.gene28358:CDS 30.0%
! GATTGCTCTTTTGTAAACTT+TGG - chr4.2:38873962-38873981 None:intergenic 30.0%
! TTTTAATGTAAATACAGGGC+TGG + chr4.2:38874770-38874789 MS.gene28358:intron 30.0%
!! ACCATTTTTGTAATCTACGA+AGG - chr4.2:38874879-38874898 None:intergenic 30.0%
!!! ACACCCACTATTTTTTATGT+GGG + chr4.2:38873565-38873584 MS.gene28358:intron 30.0%
!!! TTTCCATCAGTGATTTTTTG+AGG - chr4.2:38872616-38872635 None:intergenic 30.0%
AAACTGCAGAAAAGATTTCC+AGG + chr4.2:38873110-38873129 MS.gene28358:intron 35.0%
AACACTGAATCACTGCAAAT+AGG + chr4.2:38872460-38872479 MS.gene28358:CDS 35.0%
AACGAGAAAGAGAATCTGTA+AGG - chr4.2:38874084-38874103 None:intergenic 35.0%
AAGGGTTATACTGTCAGATT+TGG + chr4.2:38874848-38874867 MS.gene28358:CDS 35.0%
AAGTGGTTAAATACACCCTA+AGG - chr4.2:38873760-38873779 None:intergenic 35.0%
AAGTGGTTAATTACACCCTA+AGG - chr4.2:38873611-38873630 None:intergenic 35.0%
AATAGAAAAGACTTCCCAGA+AGG + chr4.2:38872490-38872509 MS.gene28358:CDS 35.0%
ACTCCCACATAAAAACTAGT+GGG + chr4.2:38873654-38873673 MS.gene28358:intron 35.0%
AGTGGAGGAATTAATCAAGA+AGG + chr4.2:38872871-38872890 MS.gene28358:CDS 35.0%
ATACTTTGCATGGTCTTTGT+TGG + chr4.2:38874808-38874827 MS.gene28358:CDS 35.0%
ATCACTTGAGGAATCACTTA+TGG + chr4.2:38874611-38874630 MS.gene28358:CDS 35.0%
ATGTAAAGGGATACTTTGCA+TGG + chr4.2:38874798-38874817 MS.gene28358:CDS 35.0%
ATTTCCAGGTTGCTTCAAAT+TGG + chr4.2:38874335-38874354 MS.gene28358:intron 35.0%
CAACAACCTCATTGATGAAT+TGG + chr4.2:38872906-38872925 MS.gene28358:CDS 35.0%
CACCTTCTAACAATAACAAG+TGG - chr4.2:38873720-38873739 None:intergenic 35.0%
CACTCCCACATAAAAAATAG+TGG - chr4.2:38873572-38873591 None:intergenic 35.0%
GTTATAGCAACATACACCTT+AGG + chr4.2:38873741-38873760 MS.gene28358:intron 35.0%
TATGCAGATGGAAAACTAAG+TGG + chr4.2:38872853-38872872 MS.gene28358:intron 35.0%
TATTCATCTTCCAGAGTTTG+AGG - chr4.2:38872979-38872998 None:intergenic 35.0%
TCTTCTGCATATCAGTATGA+AGG + chr4.2:38872532-38872551 MS.gene28358:CDS 35.0%
TGCCAAATATAAAGCCTTCT+GGG - chr4.2:38872507-38872526 None:intergenic 35.0%
TGTGTTCTAGCAAATCCTAT+AGG - chr4.2:38873696-38873715 None:intergenic 35.0%
TTATAGGTTTATGGATCCCT+TGG + chr4.2:38873886-38873905 MS.gene28358:intron 35.0%
TTTAGCAAAGCTACACCTTA+GGG + chr4.2:38873593-38873612 MS.gene28358:intron 35.0%
! ATTGATGAATTGGTGGCAAA+TGG + chr4.2:38872916-38872935 MS.gene28358:CDS 35.0%
! CAAACTCTGGAAGATGAATA+TGG + chr4.2:38872979-38872998 MS.gene28358:CDS 35.0%
! GACAATTTTGAATGGCACAA+GGG + chr4.2:38874830-38874849 MS.gene28358:CDS 35.0%
! TTCCCAGAAGGCTTTATATT+TGG + chr4.2:38872502-38872521 MS.gene28358:CDS 35.0%
! TTTTAGCAAAGCTACACCTT+AGG + chr4.2:38873592-38873611 MS.gene28358:intron 35.0%
! TTTTCATGTAAGCACCCTTT+TGG + chr4.2:38874103-38874122 MS.gene28358:intron 35.0%
!! ACCCACTTGTTATTGTTAGA+AGG + chr4.2:38873715-38873734 MS.gene28358:intron 35.0%
!! GGTTTTTGGATAATCTCCAT+TGG - chr4.2:38873912-38873931 None:intergenic 35.0%
!! GTGAAAGTATCCCATATACT+TGG - chr4.2:38872583-38872602 None:intergenic 35.0%
!! TCAAGTTTGTTATCTGAGAG+TGG - chr4.2:38873429-38873448 None:intergenic 35.0%
!!! ACACCCACTAGTTTTTATGT+GGG - chr4.2:38873660-38873679 None:intergenic 35.0%
!!! CACACCCACTATTTTTTATG+TGG + chr4.2:38873564-38873583 MS.gene28358:intron 35.0%
AGAAAGTCTCGAATTGCTCT+TGG - chr4.2:38874373-38874392 None:intergenic 40.0%
AGAGAATCTGTAAGGTAGCT+AGG - chr4.2:38874076-38874095 None:intergenic 40.0%
AGAGCTACAGACTTCATGTT+TGG + chr4.2:38873465-38873484 MS.gene28358:CDS 40.0%
AGTCAAGATGGGTATGCAAA+TGG + chr4.2:38873210-38873229 MS.gene28358:CDS 40.0%
CAACCTCATTGATGAATTGG+TGG + chr4.2:38872909-38872928 MS.gene28358:CDS 40.0%
CACTCCCACATAAAAACTAG+TGG + chr4.2:38873653-38873672 MS.gene28358:intron 40.0%
CATCGCTATAAGGAAGATGT+TGG + chr4.2:38872664-38872683 MS.gene28358:CDS 40.0%
CATGGACTTACAGTCAAGAT+GGG + chr4.2:38873199-38873218 MS.gene28358:CDS 40.0%
CTACAGACTTCATGTTTGGA+TGG + chr4.2:38873469-38873488 MS.gene28358:CDS 40.0%
GGAAGATGTTGGAATCATGA+AGG + chr4.2:38872675-38872694 MS.gene28358:CDS 40.0%
GGATAACTTTGAATGAGCCA+TGG + chr4.2:38873181-38873200 MS.gene28358:CDS 40.0%
GGGTGTGATAGGTATAACAT+TGG + chr4.2:38873386-38873405 MS.gene28358:intron 40.0%
GTATCACTCACATGATGAGT+GGG - chr4.2:38873017-38873036 None:intergenic 40.0%
GTGCCAAATATAAAGCCTTC+TGG - chr4.2:38872508-38872527 None:intergenic 40.0%
GTGTTCTAGCAAATGCCTAT+AGG + chr4.2:38873678-38873697 MS.gene28358:intron 40.0%
TGACTCGTATCATCGCTATA+AGG + chr4.2:38872654-38872673 MS.gene28358:CDS 40.0%
TGATCCATCGTTATCACTTG+AGG + chr4.2:38874599-38874618 MS.gene28358:CDS 40.0%
TGGAAGAGGACCAAGTATAT+GGG + chr4.2:38872570-38872589 MS.gene28358:CDS 40.0%
TGGGTATGCAAATGGTGAAA+TGG + chr4.2:38873218-38873237 MS.gene28358:CDS 40.0%
TGGTAACCTTGTTCTTACCA+AGG - chr4.2:38873942-38873961 None:intergenic 40.0%
TGGTTGAATCCAAATTGCAC+CGG + chr4.2:38873261-38873280 MS.gene28358:CDS 40.0%
TGTATCACTCACATGATGAG+TGG - chr4.2:38873018-38873037 None:intergenic 40.0%
TTGCCACCAATTCATCAATG+AGG - chr4.2:38872915-38872934 None:intergenic 40.0%
! ACAACCAATTTGAAGCAACC+TGG - chr4.2:38874342-38874361 None:intergenic 40.0%
! ACTCTGGAAGATGAATATGG+CGG + chr4.2:38872982-38873001 MS.gene28358:CDS 40.0%
! AGGGCTGGTGTAAATGTAAA+GGG + chr4.2:38874785-38874804 MS.gene28358:intron 40.0%
! GAACGTGATGGAAAAACCAT+TGG + chr4.2:38874215-38874234 MS.gene28358:CDS 40.0%
! GATTCCTCAAGTGATAACGA+TGG - chr4.2:38874606-38874625 None:intergenic 40.0%
! GGACAATTTTGAATGGCACA+AGG + chr4.2:38874829-38874848 MS.gene28358:CDS 40.0%
!!! CACACCCACTAGTTTTTATG+TGG - chr4.2:38873661-38873680 None:intergenic 40.0%
AGCAAAGTTCTGCATAGTCC+TGG - chr4.2:38873131-38873150 None:intergenic 45.0%
ATCAAAAGCTCTCAGGGTTG+TGG + chr4.2:38874909-38874928 MS.gene28358:CDS 45.0%
CCATCTTGACTGTAAGTCCA+TGG - chr4.2:38873201-38873220 None:intergenic 45.0%
CCATGGACTTACAGTCAAGA+TGG + chr4.2:38873198-38873217 MS.gene28358:CDS 45.0%
GCAGATGGAAAACTAAGTGG+AGG + chr4.2:38872856-38872875 MS.gene28358:intron 45.0%
GGATAATCTCCATTGGCCAA+GGG - chr4.2:38873905-38873924 None:intergenic 45.0%
GTGATTGCAGTTGAACGTGA+TGG + chr4.2:38874203-38874222 MS.gene28358:intron 45.0%
GTGGAAGAGGACCAAGTATA+TGG + chr4.2:38872569-38872588 MS.gene28358:CDS 45.0%
TCCAAAAACCATGAGAGCCT+TGG + chr4.2:38873922-38873941 MS.gene28358:CDS 45.0%
TGAGAGCCTTGGTAAGAACA+AGG + chr4.2:38873933-38873952 MS.gene28358:CDS 45.0%
TGGAAACAATGGAGACGTTG+CGG + chr4.2:38872630-38872649 MS.gene28358:CDS 45.0%
TGGATAATCTCCATTGGCCA+AGG - chr4.2:38873906-38873925 None:intergenic 45.0%
TGTTCTTACCAAGGCTCTCA+TGG - chr4.2:38873933-38873952 None:intergenic 45.0%
TTGAATCCAAATTGCACCGG+CGG + chr4.2:38873264-38873283 MS.gene28358:CDS 45.0%
TTGGGATCTTCCTCAAACTC+TGG + chr4.2:38872966-38872985 MS.gene28358:CDS 45.0%
TTTATGGATCCCTTGGCCAA+TGG + chr4.2:38873893-38873912 MS.gene28358:CDS 45.0%
! ACCAAGGCTCTCATGGTTTT+TGG - chr4.2:38873926-38873945 None:intergenic 45.0%
! CAGGGCTGGTGTAAATGTAA+AGG + chr4.2:38874784-38874803 MS.gene28358:intron 45.0%
! GTCTCTCAAAAGGGTGTGAT+AGG + chr4.2:38873375-38873394 MS.gene28358:intron 45.0%
! TATGAAGGTGCAGCAAGTGA+AGG + chr4.2:38872547-38872566 MS.gene28358:CDS 45.0%
!! CTATTAAAAATTTAAATTAA+TGG + chr4.2:38874160-38874179 MS.gene28358:intron 5.0%
!! TAATTAAAAATAATGAAATA+TGG - chr4.2:38872817-38872836 None:intergenic 5.0%
!! TTATCATATATATATATATA+GGG - chr4.2:38873844-38873863 None:intergenic 5.0%
GAGCAATCAAGGCTAGTGAG+TGG + chr4.2:38873974-38873993 MS.gene28358:CDS 50.0%
GATGCACCTCAGTTGAGCAA+TGG + chr4.2:38874046-38874065 MS.gene28358:CDS 50.0%
GCAAATGGTGAAATGGCACC+AGG + chr4.2:38873225-38873244 MS.gene28358:CDS 50.0%
GGTTTGCCATTGCTCAACTG+AGG - chr4.2:38874055-38874074 None:intergenic 50.0%
!! AACCAAGAAGAGCATCGACC+TGG - chr4.2:38873246-38873265 None:intergenic 50.0%
CACCAGGTCGATGCTCTTCT+TGG + chr4.2:38873241-38873260 MS.gene28358:CDS 55.0%
GAATCACCGCCGGTGCAATT+TGG - chr4.2:38873273-38873292 None:intergenic 55.0%
GCAGCAAGTGAAGGTGGAAG+AGG + chr4.2:38872556-38872575 MS.gene28358:CDS 55.0%
TTCCGTTCCAGAATCACCGC+CGG - chr4.2:38873283-38873302 None:intergenic 55.0%
! AATTGCACCGGCGGTGATTC+TGG + chr4.2:38873273-38873292 MS.gene28358:CDS 55.0%
! GAAGGTGCAGCAAGTGAAGG+TGG + chr4.2:38872550-38872569 MS.gene28358:CDS 55.0%
! CACCGGCGGTGATTCTGGAA+CGG + chr4.2:38873278-38873297 MS.gene28358:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 38872379 38874985 38872379 ID=MS.gene28358
chr4.2 mRNA 38872379 38874985 38872379 ID=MS.gene28358.t1;Parent=MS.gene28358
chr4.2 exon 38872379 38872751 38872379 ID=MS.gene28358.t1.exon1;Parent=MS.gene28358.t1
chr4.2 CDS 38872379 38872751 38872379 ID=cds.MS.gene28358.t1;Parent=MS.gene28358.t1
chr4.2 exon 38872860 38873025 38872860 ID=MS.gene28358.t1.exon2;Parent=MS.gene28358.t1
chr4.2 CDS 38872860 38873025 38872860 ID=cds.MS.gene28358.t1;Parent=MS.gene28358.t1
chr4.2 exon 38873119 38873347 38873119 ID=MS.gene28358.t1.exon3;Parent=MS.gene28358.t1
chr4.2 CDS 38873119 38873347 38873119 ID=cds.MS.gene28358.t1;Parent=MS.gene28358.t1
chr4.2 exon 38873387 38873490 38873387 ID=MS.gene28358.t1.exon4;Parent=MS.gene28358.t1
chr4.2 CDS 38873387 38873490 38873387 ID=cds.MS.gene28358.t1;Parent=MS.gene28358.t1
chr4.2 exon 38873892 38874109 38873892 ID=MS.gene28358.t1.exon5;Parent=MS.gene28358.t1
chr4.2 CDS 38873892 38874109 38873892 ID=cds.MS.gene28358.t1;Parent=MS.gene28358.t1
chr4.2 exon 38874213 38874244 38874213 ID=MS.gene28358.t1.exon6;Parent=MS.gene28358.t1
chr4.2 CDS 38874213 38874244 38874213 ID=cds.MS.gene28358.t1;Parent=MS.gene28358.t1
chr4.2 exon 38874343 38874445 38874343 ID=MS.gene28358.t1.exon7;Parent=MS.gene28358.t1
chr4.2 CDS 38874343 38874445 38874343 ID=cds.MS.gene28358.t1;Parent=MS.gene28358.t1
chr4.2 exon 38874583 38874691 38874583 ID=MS.gene28358.t1.exon8;Parent=MS.gene28358.t1
chr4.2 CDS 38874583 38874691 38874583 ID=cds.MS.gene28358.t1;Parent=MS.gene28358.t1
chr4.2 exon 38874787 38874985 38874787 ID=MS.gene28358.t1.exon9;Parent=MS.gene28358.t1
chr4.2 CDS 38874787 38874985 38874787 ID=cds.MS.gene28358.t1;Parent=MS.gene28358.t1
Gene Sequence

>MS.gene28358

ATGAGATATTTCCTATTTATTCTCTTCTTGTCTTTAGTTTCAACATTCATCATTACTTTAACAGAAGGTCAAGAAATAATTAACACTGAATCACTGCAAATAGGTTCATTGAATAGAAAAGACTTCCCAGAAGGCTTTATATTTGGCACAGCATCTTCTGCATATCAGTATGAAGGTGCAGCAAGTGAAGGTGGAAGAGGACCAAGTATATGGGATACTTTCACTCATAGATATCCTCAAAAAATCACTGATGGAAACAATGGAGACGTTGCGGTTGACTCGTATCATCGCTATAAGGAAGATGTTGGAATCATGAAGGATATGAACTTGGATGCATACAGATTTTCTATATCTTGGTCTAGAATACTCCCAAGTATGTCTAGTCAATTGAATTTATACTAATCCCTATCAATTATATGTTTATCTCTTTATAAACCATATTTCATTATTTTTAATTACTTAACTTCATTAATTTATGCAGATGGAAAACTAAGTGGAGGAATTAATCAAGAAGGAATCGACTATTACAACAACCTCATTGATGAATTGGTGGCAAATGGATTACAACCATTTGTAACACTTTTTCATTGGGATCTTCCTCAAACTCTGGAAGATGAATATGGCGGTTTCTTAAGCCCACTCATCATGTGAGTGATACAATTTCAAACTTTCAGTTAATAACTTAGTTTAAATATTTGTGGAAATGTTTCTCATTCATCTTCACATACTTTAAACTGCAGAAAAGATTTCCAGGACTATGCAGAACTTTGCTTCAAAACATTTGGAGATAGAGTAAAACATTGGATAACTTTGAATGAGCCATGGACTTACAGTCAAGATGGGTATGCAAATGGTGAAATGGCACCAGGTCGATGCTCTTCTTGGTTGAATCCAAATTGCACCGGCGGTGATTCTGGAACGGAACCTTATTTAGTTGCACATTATCAACTTCTAGCTCATGCAACAGTTGTTAATTTATACAAGACAAAGTATCAAGTCTCTCAAAAGGGTGTGATAGGTATAACATTGGTTATTAACTATTTTGTGCCACTCTCAGATAACAAACTTGATATAAAGGCTGCTGAAAGAGCTACAGACTTCATGTTTGGATGGTAAGTTGATTACAAAGATATAATCATCCAAATTAACACAAGGAGATATATATATATATGGGGTTTGCTAGAACACACCCACTATTTTTTATGTGGGAGTGTTTTAGCAAAGCTACACCTTAGGGTGTAATTAACCACTTTTTAGTTATTTACTTGCTAAAACACTCCCACATAAAAACTAGTGGGTGTGTTCTAGCAAATGCCTATAGGATTTGCTAGAACACACCCACTTGTTATTGTTAGAAGGTGTGTTATAGCAACATACACCTTAGGGTGTATTTAACCACTTTTTAGTTATTTACTTGCTAAAACACTCCCACATAAAAACTAGTGGGTGTGTTCTAGTAAACCCCTATATATATATATATGATAACTTATTCTCTTTTTTTATTTAATTATAGGTTTATGGATCCCTTGGCCAATGGAGATTATCCAAAAACCATGAGAGCCTTGGTAAGAACAAGGTTACCAAAGTTTACAAAAGAGCAATCAAGGCTAGTGAGTGGTTCATTTGATTTTATTGGCATAAATTACTACTCTTCATGCTATGCTTCTGATGCACCTCAGTTGAGCAATGGCAAACCTAGCTACCTTACAGATTCTCTTTCTCGTTTTTCATGTAAGCACCCTTTTGGCCTACTTTATATGATCTATTGATTATTAAGTTATCTATTAAAAATTTAAATTAATGGTATACATTTTTTTTTTGTGTGTGATTGCAGTTGAACGTGATGGAAAAACCATTGGTCTAAATGTAAGTGTTAAATTAACCATGAAATGTTGCAATATCTCATCTGTTAATTGGTTTTTGTCTAAATTGGTAATTAATGAATTTGGTTATTTTATTTCCAGGTTGCTTCAAATTGGTTGTATGTTTATCCAAGAGCAATTCGAGACTTTCTGATACAAGTTAAAGAAAAATACAACAACCCTTTGATTTATATTACTGAAAATGGTAAGCAAATCACTTTCATATCATGCTTTTTCTTCTTTAAATAGATAAAGTTATAGTAAAAAACTTTTTTACTTTAGCTTCTTAAAAAAAACATTTTACTCTAGATATATAACTCAACTTTTTCATAATGTGTGTAGGTATTAATGAGTACGATGATCCATCGTTATCACTTGAGGAATCACTTATGGATACGTATAGAGTTGATTATCATTATCGTCATTTATTCTATCTTAATGAGGCAATCAAGTGAGTAATTTTTTTATATATCTAAAGATCAGTCTATTTCACTTTCTTTCATGGAATTTAGCTAAACTATTTTCTTTCTTTTAATGTAAATACAGGGCTGGTGTAAATGTAAAGGGATACTTTGCATGGTCTTTGTTGGACAATTTTGAATGGCACAAGGGTTATACTGTCAGATTTGGAATGACCTTCGTAGATTACAAAAATGGTTTGAAAAGATATCAAAAGCTCTCAGGGTTGTGGTTCAAGAATTTTCTCACATCAGACAACAAAAAAGTTTACATAGATTCCATTTAA
Protein sequence

>MS.gene28358.t1

MRYFLFILFLSLVSTFIITLTEGQEIINTESLQIGSLNRKDFPEGFIFGTASSAYQYEGAASEGGRGPSIWDTFTHRYPQKITDGNNGDVAVDSYHRYKEDVGIMKDMNLDAYRFSISWSRILPNGKLSGGINQEGIDYYNNLIDELVANGLQPFVTLFHWDLPQTLEDEYGGFLSPLIIKDFQDYAELCFKTFGDRVKHWITLNEPWTYSQDGYANGEMAPGRCSSWLNPNCTGGDSGTEPYLVAHYQLLAHATVGVIGITLVINYFVPLSDNKLDIKAAERATDFMFGWFMDPLANGDYPKTMRALVRTRLPKFTKEQSRLVSGSFDFIGINYYSSCYASDAPQLSNGKPSYLTDSLSRFSFERDGKTIGLNVASNWLYVYPRAIRDFLIQVKEKYNNPLIYITENGINEYDDPSLSLEESLMDTYRVDYHYRHLFYLNEAIKAGVNVKGYFAWSLLDNFEWHKGYTVRFGMTFVDYKNGLKRYQKLSGLWFKNFLTSDNKKVYIDSI