AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene28619


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene28619.t1 MTR_7g111010 99.174 242 2 0 1 242 13 254 0.0 500
MS.gene28619.t1 MTR_7g110990 96.234 239 9 0 4 242 1 239 2.94e-173 476
MS.gene28619.t1 MTR_1g085460 85.833 240 34 0 3 242 18 257 1.50e-158 440
MS.gene28619.t1 MTR_1g026020 66.379 232 77 1 11 242 25 255 2.92e-118 338
MS.gene28619.t1 MTR_5g075320 64.126 223 76 3 15 237 29 247 1.61e-105 305
MS.gene28619.t1 MTR_7g061720 63.677 223 77 3 15 236 32 251 7.53e-105 304
MS.gene28619.t1 MTR_3g094040 64.865 222 74 3 15 236 31 248 1.11e-104 303
MS.gene28619.t1 MTR_3g094060 64.865 222 74 3 15 236 31 248 1.16e-104 303
MS.gene28619.t1 MTR_2g097030 64.574 223 75 3 15 236 34 253 1.81e-104 303
MS.gene28619.t1 MTR_5g041700 65.766 222 72 3 15 236 25 242 3.29e-104 302
MS.gene28619.t1 MTR_3g435430 64.474 228 76 4 15 242 25 247 6.20e-104 301
MS.gene28619.t1 MTR_5g079950 64.286 224 76 3 14 236 32 252 1.76e-103 300
MS.gene28619.t1 MTR_4g081950 65.315 222 73 3 15 236 61 278 2.36e-103 301
MS.gene28619.t1 MTR_7g011060 64.035 228 70 4 9 236 20 235 1.65e-100 292
MS.gene28619.t1 MTR_3g466790 61.181 237 88 3 1 237 18 250 4.95e-100 291
MS.gene28619.t1 MTR_3g466760 60.759 237 89 3 1 237 24 256 4.07e-98 287
MS.gene28619.t1 MTR_4g063975 62.332 223 80 3 15 236 32 251 2.98e-96 282
MS.gene28619.t1 MTR_6g012520 64.414 222 67 4 15 236 28 237 1.52e-91 270
MS.gene28619.t1 MTR_5g011210 55.217 230 97 4 15 242 37 262 1.47e-86 258
MS.gene28619.t1 MTR_4g102450 55.459 229 98 3 15 242 36 261 9.69e-86 256
MS.gene28619.t1 MTR_7g013120 51.965 229 105 3 15 242 29 253 5.68e-85 253
MS.gene28619.t1 MTR_7g013140 51.852 243 111 4 1 242 14 251 5.04e-84 251
MS.gene28619.t1 MTR_4g124340 50.673 223 105 3 15 236 61 279 6.25e-84 252
MS.gene28619.t1 MTR_7g013150 53.275 229 101 4 15 242 30 253 2.50e-83 249
MS.gene28619.t1 MTR_8g464350 51.965 229 104 4 15 242 31 254 1.90e-81 244
MS.gene28619.t1 MTR_8g058990 50.218 229 108 4 15 242 30 253 6.47e-78 235
MS.gene28619.t1 MTR_0040s0140 50.446 224 107 3 15 237 32 252 5.94e-76 230
MS.gene28619.t1 MTR_2g090255 49.107 224 110 3 15 237 28 248 3.23e-73 223
MS.gene28619.t1 MTR_5g076950 48.444 225 109 3 15 237 41 260 7.96e-72 220
MS.gene28619.t1 MTR_3g064510 46.725 229 115 5 15 237 53 280 5.92e-68 211
MS.gene28619.t1 MTR_6g012500 58.333 96 39 1 141 236 181 275 3.69e-32 119
MS.gene28619.t1 MTR_0008s0120 33.333 228 92 6 15 242 32 199 9.07e-31 113
MS.gene28619.t1 MTR_2g090270 30.962 239 139 8 15 242 35 258 2.36e-30 114
MS.gene28619.t1 MTR_2g090265 31.799 239 137 9 15 242 33 256 8.50e-29 109
MS.gene28619.t1 MTR_0122s0020 31.381 239 138 9 15 242 41 264 4.41e-26 102
MS.gene28619.t1 MTR_1g085365 81.818 55 10 0 186 240 4 58 5.20e-26 96.7
MS.gene28619.t1 MTR_0118s0070 33.195 241 125 11 20 242 53 275 1.25e-25 101
MS.gene28619.t1 MTR_4g028640 31.765 255 135 12 5 242 32 264 2.67e-24 97.8
MS.gene28619.t1 MTR_0809s0010 30.041 243 128 12 15 238 47 266 2.83e-21 90.1
MS.gene28619.t1 MTR_4g099370 27.600 250 156 10 3 242 16 250 1.08e-19 85.5
MS.gene28619.t1 MTR_5g013440 27.039 233 149 9 18 242 32 251 4.00e-19 84.0
MS.gene28619.t1 MTR_4g075790 31.224 237 136 11 16 242 24 243 1.32e-18 82.4
MS.gene28619.t1 MTR_4g098930 29.362 235 142 10 17 241 31 251 1.42e-15 73.9
MS.gene28619.t1 MTR_0122s0020 32.386 176 93 10 15 179 41 201 6.43e-14 68.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene28619.t1 AT4G01630 79.167 240 50 0 3 242 16 255 2.81e-146 409
MS.gene28619.t1 AT1G20190 67.857 224 71 1 19 242 30 252 2.31e-112 323
MS.gene28619.t1 AT2G40610 65.022 223 75 2 15 237 30 249 5.90e-107 309
MS.gene28619.t1 AT5G05290 61.435 223 83 2 15 237 32 251 7.08e-106 306
MS.gene28619.t1 AT1G69530 64.865 222 74 3 15 236 27 244 6.55e-103 299
MS.gene28619.t1 AT1G69530 64.865 222 74 3 15 236 27 244 6.55e-103 299
MS.gene28619.t1 AT1G69530 64.865 222 74 3 15 236 27 244 6.86e-103 300
MS.gene28619.t1 AT3G55500 63.229 223 78 3 15 236 34 253 4.68e-102 297
MS.gene28619.t1 AT2G03090 62.500 224 80 3 13 236 28 247 5.13e-102 296
MS.gene28619.t1 AT2G39700 62.946 224 79 3 14 236 30 250 1.98e-101 295
MS.gene28619.t1 AT5G02260 62.332 223 80 3 15 236 32 251 8.02e-101 294
MS.gene28619.t1 AT2G28950 62.780 223 79 3 15 236 31 250 1.78e-100 293
MS.gene28619.t1 AT2G37640 62.500 224 80 3 15 237 36 256 1.89e-100 293
MS.gene28619.t1 AT5G56320 58.898 236 93 3 1 236 15 246 6.38e-100 291
MS.gene28619.t1 AT5G56320 58.898 236 93 3 1 236 15 246 6.38e-100 291
MS.gene28619.t1 AT5G56320 58.898 236 93 3 1 236 15 246 3.88e-99 291
MS.gene28619.t1 AT3G29030 60.360 222 77 3 15 236 38 248 1.70e-97 285
MS.gene28619.t1 AT1G26770 63.964 222 76 3 15 236 26 243 8.24e-94 276
MS.gene28619.t1 AT1G26770 63.964 222 76 3 15 236 36 253 1.02e-93 276
MS.gene28619.t1 AT1G20190 65.285 193 66 1 50 242 2 193 1.60e-91 268
MS.gene28619.t1 AT3G15370 56.000 225 93 2 15 238 27 246 1.57e-89 265
MS.gene28619.t1 AT1G69530 63.547 203 68 4 15 217 27 223 8.13e-89 263
MS.gene28619.t1 AT1G69530 65.641 195 61 4 15 209 27 215 1.28e-88 261
MS.gene28619.t1 AT2G37640 59.709 206 79 3 32 236 28 230 4.87e-87 258
MS.gene28619.t1 AT1G12560 54.585 229 99 4 15 242 37 261 1.57e-85 255
MS.gene28619.t1 AT3G15370 49.412 255 93 3 15 238 27 276 7.61e-83 249
MS.gene28619.t1 AT5G39270 51.754 228 104 4 15 242 58 279 6.94e-81 244
MS.gene28619.t1 AT5G39310 49.787 235 111 5 9 242 84 312 2.09e-79 241
MS.gene28619.t1 AT5G39300 50.439 228 107 4 15 242 55 276 2.11e-79 240
MS.gene28619.t1 AT5G39290 51.316 228 105 4 15 242 58 279 2.64e-79 240
MS.gene28619.t1 AT1G62980 49.388 245 117 6 1 242 16 256 4.44e-79 238
MS.gene28619.t1 AT5G39280 50.000 228 108 4 15 242 54 275 7.45e-79 239
MS.gene28619.t1 AT3G03220 45.333 225 116 3 15 237 40 259 9.47e-69 213
MS.gene28619.t1 AT4G38210 48.673 226 110 4 15 237 28 250 1.36e-68 212
MS.gene28619.t1 AT5G39260 45.852 229 112 5 15 242 45 262 2.00e-62 196
MS.gene28619.t1 AT2G20750 33.333 246 140 8 9 242 34 267 4.85e-31 115
MS.gene28619.t1 AT4G28250 30.556 252 148 8 3 242 24 260 5.77e-31 115
MS.gene28619.t1 AT1G65680 34.711 242 129 11 15 242 47 273 1.26e-29 112
MS.gene28619.t1 AT4G28250 29.482 251 146 8 3 242 24 254 1.22e-27 106
MS.gene28619.t1 AT2G45110 34.156 243 123 11 18 242 36 259 1.43e-24 98.6
MS.gene28619.t1 AT1G65681 31.004 229 125 11 29 242 12 222 2.92e-21 89.0
MS.gene28619.t1 AT2G20750 33.516 182 98 7 9 179 34 203 2.45e-19 83.6
MS.gene28619.t1 AT4G28250 28.646 192 107 7 3 184 24 195 7.25e-17 77.0
MS.gene28619.t1 AT4G28250 28.877 187 103 7 3 179 24 190 3.33e-16 75.1
MS.gene28619.t1 AT3G60570 29.098 244 137 10 15 242 34 257 1.03e-15 74.7
MS.gene28619.t1 AT4G17030 25.714 245 163 8 3 240 15 247 1.80e-15 73.9
MS.gene28619.t1 AT4G38400 27.354 223 123 9 38 242 47 248 4.44e-15 73.2
MS.gene28619.t1 AT3G45970 28.696 230 122 11 33 242 14 221 2.05e-14 70.9
MS.gene28619.t1 AT3G45960 27.778 216 131 10 38 242 46 247 9.85e-13 66.2
MS.gene28619.t1 AT3G45970 28.000 225 120 11 38 242 46 248 8.38e-12 63.9
MS.gene28619.t1 AT3G60570 28.319 226 126 10 33 242 4 209 1.29e-11 62.8

Find 0 sgRNAs with CRISPR-Local

Find 74 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACATAAGATTTACTATAAA+TGG + 50573:14852-14871 MS.gene28619:CDS 15.0%
!! ACATAAGATTTACTATAAAT+GGG + 50573:14853-14872 MS.gene28619:CDS 15.0%
!! AAGGATCAAAAAAGAATAAA+TGG + 50573:14946-14965 MS.gene28619:CDS 20.0%
!! AGGATCAAAAAAGAATAAAT+GGG + 50573:14947-14966 MS.gene28619:CDS 20.0%
!! TTAGAATTCAACTCAAAAAT+GGG + 50573:15039-15058 MS.gene28619:CDS 20.0%
!!! AAGATTTAATTGTCATTTAG+CGG - 50573:14770-14789 None:intergenic 20.0%
!!! ATTTCTAAAGTTTGGATAAA+AGG + 50573:14927-14946 MS.gene28619:CDS 20.0%
!!! CTTAATTTTGATTAACTTGT+AGG + 50573:14331-14350 MS.gene28619:intron 20.0%
!!! TTTAGAATTCAACTCAAAAA+TGG + 50573:15038-15057 MS.gene28619:CDS 20.0%
! AATATACCTCCTATACAAAA+TGG - 50573:14662-14681 None:intergenic 25.0%
! ATATACCTCCTATACAAAAT+GGG - 50573:14661-14680 None:intergenic 25.0%
!!! AATTTTGATTAACTTGTAGG+TGG + 50573:14334-14353 MS.gene28619:intron 25.0%
AATCTATACACAGATGGATA+TGG + 50573:14373-14392 MS.gene28619:CDS 30.0%
TATGGAAATCTATACACAGA+TGG + 50573:14367-14386 MS.gene28619:CDS 30.0%
TGTTGTATTTATGCAGAGTT+GGG + 50573:14814-14833 MS.gene28619:intron 30.0%
TTGTTGTATTTATGCAGAGT+TGG + 50573:14813-14832 MS.gene28619:intron 30.0%
! AAGAATTTGACTTTGTCGTA+GGG - 50573:14696-14715 None:intergenic 30.0%
! GAATTGTTCCCATTTTGTAT+AGG + 50573:14650-14669 MS.gene28619:CDS 30.0%
! TAAGAATTTGACTTTGTCGT+AGG - 50573:14697-14716 None:intergenic 30.0%
ACATATCAAAGTGAGGTCTA+GGG - 50573:14591-14610 None:intergenic 35.0%
AGTACTGCTCTCTTTAATGA+TGG + 50573:14415-14434 MS.gene28619:CDS 35.0%
ATTTGTGGCAGTAATAGTGA+TGG - 50573:14515-14534 None:intergenic 35.0%
GAACCAATGTCAATGAATTG+GGG + 50573:14969-14988 MS.gene28619:CDS 35.0%
GGAACCAATGTCAATGAATT+GGG + 50573:14968-14987 MS.gene28619:CDS 35.0%
GTACTGCTCTCTTTAATGAT+GGG + 50573:14416-14435 MS.gene28619:CDS 35.0%
GTACTTAGCTATAGTCTGAA+AGG - 50573:14623-14642 None:intergenic 35.0%
TTTGACCATTGAGATAGCTT+AGG - 50573:15010-15029 None:intergenic 35.0%
! GTTTGGTGGACAAAAATTTG+TGG - 50573:14530-14549 None:intergenic 35.0%
! TTGGTGCTTATAAGCAATGT+AGG + 50573:14891-14910 MS.gene28619:CDS 35.0%
! TTGTTCCCATTTTGTATAGG+AGG + 50573:14653-14672 MS.gene28619:CDS 35.0%
! TTTAATGATGGGAAGTCATG+TGG + 50573:14427-14446 MS.gene28619:CDS 35.0%
!! GTGGAGAGATTTCTAAAGTT+TGG + 50573:14919-14938 MS.gene28619:CDS 35.0%
!! TGGGAGAGACTATTTTGAAT+TGG + 50573:14872-14891 MS.gene28619:CDS 35.0%
!!! GAACGTTACTTTTGTAAGAC+TGG - 50573:15112-15131 None:intergenic 35.0%
ACCAACCACCATTGTCATTA+GGG - 50573:14561-14580 None:intergenic 40.0%
ACTATAGCTAAGTACAGAGC+TGG + 50573:14628-14647 MS.gene28619:CDS 40.0%
ATATCAAAGTGAGGTCTAGG+GGG - 50573:14589-14608 None:intergenic 40.0%
CAAAGCCTAAGCTATCTCAA+TGG + 50573:15002-15021 MS.gene28619:CDS 40.0%
CAAATTACCCATTGTTCCTG+AGG - 50573:14233-14252 None:intergenic 40.0%
CAAGCTCATGCAACTTTCTA+TGG + 50573:14196-14215 MS.gene28619:CDS 40.0%
CATATCAAAGTGAGGTCTAG+GGG - 50573:14590-14609 None:intergenic 40.0%
CTTATAAGCAATGTAGGTGG+AGG + 50573:14897-14916 MS.gene28619:CDS 40.0%
GACATATCAAAGTGAGGTCT+AGG - 50573:14592-14611 None:intergenic 40.0%
GGGAACCAATGTCAATGAAT+TGG + 50573:14967-14986 MS.gene28619:CDS 40.0%
GGTTGAGACATATCAAAGTG+AGG - 50573:14598-14617 None:intergenic 40.0%
GTGCTTATAAGCAATGTAGG+TGG + 50573:14894-14913 MS.gene28619:CDS 40.0%
TCAATGTTGCACCTTCCAAT+TGG + 50573:15078-15097 MS.gene28619:CDS 40.0%
TCAGAGTAGAATCTGCTGTT+TGG + 50573:14170-14189 MS.gene28619:CDS 40.0%
TTAGCTATAGTCTGAAAGGC+AGG - 50573:14619-14638 None:intergenic 40.0%
TTTGCACTCCCTAATGACAA+TGG + 50573:14550-14569 MS.gene28619:CDS 40.0%
! ATCAGATAGTTTGTGACGCA+AGG + 50573:14458-14477 MS.gene28619:CDS 40.0%
! CAATGAATTGGGGTGCTAAT+TGG + 50573:14979-14998 MS.gene28619:CDS 40.0%
! TCATTAGGGAGTGCAAAGTT+TGG - 50573:14547-14566 None:intergenic 40.0%
AACTTGTAGGTGGTGCATGT+GGG + 50573:14344-14363 MS.gene28619:intron 45.0%
AAGTGATGCCTCAGGAACAA+TGG + 50573:14222-14241 MS.gene28619:CDS 45.0%
AGAGTTGGGTGCAAAAGAAG+TGG + 50573:14828-14847 MS.gene28619:intron 45.0%
AGTGATGCCTCAGGAACAAT+GGG + 50573:14223-14242 MS.gene28619:CDS 45.0%
ATAAGCAATGTAGGTGGAGG+TGG + 50573:14900-14919 MS.gene28619:CDS 45.0%
CACCAACCACCATTGTCATT+AGG - 50573:14562-14581 None:intergenic 45.0%
GCACCCCAATTCATTGACAT+TGG - 50573:14975-14994 None:intergenic 45.0%
GCACCTTCCAATTGGAGATT+TGG + 50573:15086-15105 MS.gene28619:CDS 45.0%
GCTCATGCAACTTTCTATGG+TGG + 50573:14199-14218 MS.gene28619:CDS 45.0%
TCCCTAATGACAATGGTGGT+TGG + 50573:14557-14576 MS.gene28619:CDS 45.0%
TGGCCAAATCTCCAATTGGA+AGG - 50573:15092-15111 None:intergenic 45.0%
! AATGATGGGAAGTCATGTGG+TGG + 50573:14430-14449 MS.gene28619:CDS 45.0%
! AGACTGGCCAAATCTCCAAT+TGG - 50573:15096-15115 None:intergenic 45.0%
! TAACTTGTAGGTGGTGCATG+TGG + 50573:14343-14362 MS.gene28619:intron 45.0%
! TTAGGGAGTGCAAAGTTTGG+TGG - 50573:14544-14563 None:intergenic 45.0%
ACGCAAGGCAAGTTCCTCAA+TGG + 50573:14473-14492 MS.gene28619:CDS 50.0%
GCACTCCCTAATGACAATGG+TGG + 50573:14553-14572 MS.gene28619:CDS 50.0%
GTTCCTCAATGGTGTCTCAG+AGG + 50573:14484-14503 MS.gene28619:CDS 50.0%
! TATGGTGGAAGTGATGCCTC+AGG + 50573:14214-14233 MS.gene28619:CDS 50.0%
GTAGGTGGTGCATGTGGGTA+TGG + 50573:14349-14368 MS.gene28619:intron 55.0%
!! GTGCCTCTGAGACACCATTG+AGG - 50573:14490-14509 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
50573 gene 14148 15139 14148 ID=MS.gene28619
50573 mRNA 14148 15139 14148 ID=MS.gene28619.t1;Parent=MS.gene28619
50573 exon 14148 14244 14148 ID=MS.gene28619.t1.exon1;Parent=MS.gene28619.t1
50573 CDS 14148 14244 14148 ID=cds.MS.gene28619.t1;Parent=MS.gene28619.t1
50573 exon 14353 14674 14353 ID=MS.gene28619.t1.exon2;Parent=MS.gene28619.t1
50573 CDS 14353 14674 14353 ID=cds.MS.gene28619.t1;Parent=MS.gene28619.t1
50573 exon 14830 15139 14830 ID=MS.gene28619.t1.exon3;Parent=MS.gene28619.t1
50573 CDS 14830 15139 14830 ID=cds.MS.gene28619.t1;Parent=MS.gene28619.t1
Gene Sequence

>MS.gene28619

ATGAATATGGTTACTAAAGAACTCAGAGTAGAATCTGCTGTTTGGCAGCAAGCTCATGCAACTTTCTATGGTGGAAGTGATGCCTCAGGAACAATGGGTAATTTGCAACTTCTTTTGATTAACTTTATTCAAGTCTTAAGACAAATTAAAGTATTAATCAAATTATGATAGTTAAGCATATAACTTAATTTTGATTAACTTGTAGGTGGTGCATGTGGGTATGGAAATCTATACACAGATGGATATGGTATAAAATCAGCAGCATTGAGTACTGCTCTCTTTAATGATGGGAAGTCATGTGGTGGTTGCTATCAGATAGTTTGTGACGCAAGGCAAGTTCCTCAATGGTGTCTCAGAGGCACTTCCATCACTATTACTGCCACAAATTTTTGTCCACCAAACTTTGCACTCCCTAATGACAATGGTGGTTGGTGTAATCCCCCTAGACCTCACTTTGATATGTCTCAACCTGCCTTTCAGACTATAGCTAAGTACAGAGCTGGAATTGTTCCCATTTTGTATAGGAGGTATATTTTAAATTTCTTCCCTACGACAAAGTCAAATTCTTATCACTGAGCTGACACTTCATAGAAAAACAAATATTTATAGTGATTACACTCCGCTAAATGACAATTAAATCTTAAATATGTATTTGATACAAAGTTTTGTTGTATTTATGCAGAGTTGGGTGCAAAAGAAGTGGAAACATAAGATTTACTATAAATGGGAGAGACTATTTTGAATTGGTGCTTATAAGCAATGTAGGTGGAGGTGGAGAGATTTCTAAAGTTTGGATAAAAGGATCAAAAAAGAATAAATGGGAACCAATGTCAATGAATTGGGGTGCTAATTGGCAAAGCCTAAGCTATCTCAATGGTCAAAGTTTATCATTTAGAATTCAACTCAAAAATGGGAAGACTCGCACAGCTATCAATGTTGCACCTTCCAATTGGAGATTTGGCCAGTCTTACAAAAGTAACGTTCAGTTCTGA

Protein sequence

>MS.gene28619.t1

MNMVTKELRVESAVWQQAHATFYGGSDASGTMGGACGYGNLYTDGYGIKSAALSTALFNDGKSCGGCYQIVCDARQVPQWCLRGTSITITATNFCPPNFALPNDNGGWCNPPRPHFDMSQPAFQTIAKYRAGIVPILYRRVGCKRSGNIRFTINGRDYFELVLISNVGGGGEISKVWIKGSKKNKWEPMSMNWGANWQSLSYLNGQSLSFRIQLKNGKTRTAINVAPSNWRFGQSYKSNVQF