Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30668.t1 | XP_019442796.1 | 67.5 | 123 | 12 | 1 | 1 | 95 | 87 | 209 | 1.10E-34 | 155.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30668.t1 | A0A072UHR4 | 60.5 | 152 | 3 | 1 | 1 | 95 | 90 | 241 | 1.8e-34 | 154.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049322 | MS.gene30668 | 0.806176 | 9.34E-50 | -1.69E-46 |
| MS.gene049822 | MS.gene30668 | 0.801654 | 8.13E-49 | -1.69E-46 |
| MS.gene050114 | MS.gene30668 | 0.821985 | 3.02E-53 | -1.69E-46 |
| MS.gene050317 | MS.gene30668 | 0.814884 | 1.23E-51 | -1.69E-46 |
| MS.gene051245 | MS.gene30668 | 0.845797 | 3.25E-59 | -1.69E-46 |
| MS.gene051756 | MS.gene30668 | 0.81318 | 2.92E-51 | -1.69E-46 |
| MS.gene052856 | MS.gene30668 | 0.800919 | 1.15E-48 | -1.69E-46 |
| MS.gene053109 | MS.gene30668 | 0.80696 | 6.38E-50 | -1.69E-46 |
| MS.gene055441 | MS.gene30668 | 0.8105 | 1.12E-50 | -1.69E-46 |
| MS.gene057451 | MS.gene30668 | -0.827996 | 1.15E-54 | -1.69E-46 |
| MS.gene057452 | MS.gene30668 | -0.830622 | 2.65E-55 | -1.69E-46 |
| MS.gene058182 | MS.gene30668 | 0.83545 | 1.67E-56 | -1.69E-46 |
| MS.gene058238 | MS.gene30668 | 0.817449 | 3.28E-52 | -1.69E-46 |
| MS.gene060084 | MS.gene30668 | 0.847101 | 1.43E-59 | -1.69E-46 |
| MS.gene060297 | MS.gene30668 | 0.831031 | 2.10E-55 | -1.69E-46 |
| MS.gene060869 | MS.gene30668 | 0.818037 | 2.42E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30668.t1 | MTR_6g017260 | 53.947 | 152 | 13 | 3 | 1 | 95 | 90 | 241 | 7.98e-39 | 130 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30668.t1 | AT5G38660 | 46.711 | 152 | 24 | 3 | 1 | 95 | 94 | 245 | 1.06e-32 | 115 |
| MS.gene30668.t1 | AT5G38660 | 46.711 | 152 | 24 | 3 | 1 | 95 | 94 | 245 | 1.12e-31 | 115 |
Find 0 sgRNAs with CRISPR-Local
Find 91 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TCAATAAAAAAAAATAATTG+AGG | - | 42737:21664-21683 | None:intergenic | 10.0% |
| !! | TTAATGTTAATCAAGTTAAA+AGG | + | 42737:21080-21099 | MS.gene30668:intron | 15.0% |
| !!! | GTGTATTTAAAACAAATAAA+AGG | - | 42737:20939-20958 | None:intergenic | 15.0% |
| !! | AGAAGAAAGATTGAATATAT+TGG | - | 42737:21503-21522 | None:intergenic | 20.0% |
| !! | ATAAAAGGAAAACAAACATA+AGG | - | 42737:20924-20943 | None:intergenic | 20.0% |
| !! | TAAATCTATATCTTTAGTGT+TGG | - | 42737:21393-21412 | None:intergenic | 20.0% |
| !!! | TAATAATATGCCTATGTTTT+AGG | + | 42737:22187-22206 | MS.gene30668:intron | 20.0% |
| ! | AAACATAGGCATATTATTAG+AGG | - | 42737:22186-22205 | None:intergenic | 25.0% |
| ! | ACAAGATGGAAAATATTGTT+TGG | + | 42737:22214-22233 | MS.gene30668:CDS | 25.0% |
| ! | ATGTTAATCAAGTTAAAAGG+TGG | + | 42737:21083-21102 | MS.gene30668:intron | 25.0% |
| ! | ATTATCTGCTTTATGTTTCA+GGG | + | 42737:21996-22015 | MS.gene30668:intron | 25.0% |
| ! | CTATAATGATATATCGATGA+AGG | - | 42737:20894-20913 | None:intergenic | 25.0% |
| ! | TATAACTTACTAGACCATAT+TGG | - | 42737:21631-21650 | None:intergenic | 25.0% |
| ! | TATTATCTGCTTTATGTTTC+AGG | + | 42737:21995-22014 | MS.gene30668:intron | 25.0% |
| ! | TCAGCTATAATGTTAACATA+TGG | + | 42737:21007-21026 | MS.gene30668:CDS | 25.0% |
| ! | TTATTAAAGCTTACCTACTA+GGG | - | 42737:21843-21862 | None:intergenic | 25.0% |
| ! | TTTCTTCTAATCACTTAAAG+AGG | + | 42737:21515-21534 | MS.gene30668:intron | 25.0% |
| !!! | TTGTTTTAAATACACAGCTA+TGG | + | 42737:20944-20963 | MS.gene30668:intron | 25.0% |
| ACTTATTAGAGCTTATTTGC+TGG | - | 42737:21720-21739 | None:intergenic | 30.0% | |
| AGCATACATTAATATTCTCC+CGG | + | 42737:20973-20992 | MS.gene30668:CDS | 30.0% | |
| ATTCATCTTAAAGGTCTGAT+TGG | - | 42737:21747-21766 | None:intergenic | 30.0% | |
| CTAGAACCAATTCATCTTAA+AGG | - | 42737:21756-21775 | None:intergenic | 30.0% | |
| CTTATTAAAGCTTACCTACT+AGG | - | 42737:21844-21863 | None:intergenic | 30.0% | |
| GAAGATATCAGAAAGTAGTA+AGG | - | 42737:21908-21927 | None:intergenic | 30.0% | |
| GGATGAATAACATTTATAGG+AGG | + | 42737:21589-21608 | MS.gene30668:intron | 30.0% | |
| GTCAACATTAGAGATAGATT+TGG | + | 42737:22106-22125 | MS.gene30668:intron | 30.0% | |
| TAACATTATAGCTGACCATT+CGG | - | 42737:21002-21021 | None:intergenic | 30.0% | |
| TCCTAAAGGTCTATTTGAAT+TGG | - | 42737:21867-21886 | None:intergenic | 30.0% | |
| TGGAAAATATTGTTTGGTCT+TGG | + | 42737:22220-22239 | MS.gene30668:CDS | 30.0% | |
| TGGGGATGAATAACATTTAT+AGG | + | 42737:21586-21605 | MS.gene30668:intron | 30.0% | |
| ! | AAAGTTGTCAGATTTTGAAC+AGG | + | 42737:22262-22281 | MS.gene30668:CDS | 30.0% |
| ! | AAGTCTTATCCAACTAAGTT+GGG | - | 42737:22142-22161 | None:intergenic | 30.0% |
| ! | AAGTTGTCAGATTTTGAACA+GGG | + | 42737:22263-22282 | MS.gene30668:CDS | 30.0% |
| ! | ATATTGTGACACTCTTTTCT+TGG | + | 42737:21422-21441 | MS.gene30668:intron | 30.0% |
| ! | TAAATACACAGCTATGGTTT+TGG | + | 42737:20950-20969 | MS.gene30668:intron | 30.0% |
| !! | ATCAGACCTTTAAGATGAAT+TGG | + | 42737:21747-21766 | MS.gene30668:intron | 30.0% |
| !! | TTGAAACGGATTTTCCAATA+TGG | + | 42737:21614-21633 | MS.gene30668:intron | 30.0% |
| ATGTTACCAGATATCGCTAT+GGG | + | 42737:21567-21586 | MS.gene30668:intron | 35.0% | |
| GCATACATTAATATTCTCCC+GGG | + | 42737:20974-20993 | MS.gene30668:CDS | 35.0% | |
| GCCAATTCAAATAGACCTTT+AGG | + | 42737:21863-21882 | MS.gene30668:intron | 35.0% | |
| TCACGAACCATTTGTAGAAT+GGG | - | 42737:22047-22066 | None:intergenic | 35.0% | |
| TCTTGTGTGACCTAAAACAT+AGG | - | 42737:22200-22219 | None:intergenic | 35.0% | |
| TGCTTTATGTTTCAGGGAAA+TGG | + | 42737:22002-22021 | MS.gene30668:intron | 35.0% | |
| TTCACGAACCATTTGTAGAA+TGG | - | 42737:22048-22067 | None:intergenic | 35.0% | |
| TTTGAGTGTACAAATCGTGT+CGG | - | 42737:21240-21259 | None:intergenic | 35.0% | |
| ! | AGGCTCTTTTGTGCTTTTAT+AGG | + | 42737:21535-21554 | MS.gene30668:intron | 35.0% |
| ! | CTTTTGTGCTTTTATAGGTG+AGG | + | 42737:21540-21559 | MS.gene30668:intron | 35.0% |
| ! | GAAGTCTTATCCAACTAAGT+TGG | - | 42737:22143-22162 | None:intergenic | 35.0% |
| ! | TTCTTTGTGGTTCTATGCAT+TGG | + | 42737:21307-21326 | MS.gene30668:intron | 35.0% |
| !! | ATGTTTTAGGTCACACAAGA+TGG | + | 42737:22200-22219 | MS.gene30668:intron | 35.0% |
| !! | ATTTATAGGAGGCGTTGAAA+CGG | + | 42737:21600-21619 | MS.gene30668:intron | 35.0% |
| !! | CAAATAGACCTTTAGGATGA+AGG | + | 42737:21870-21889 | MS.gene30668:intron | 35.0% |
| !! | TAAAAAGTGCTTACCCTAGT+AGG | + | 42737:21827-21846 | MS.gene30668:intron | 35.0% |
| !! | TAAGCACTTTTTAGACCATC+TGG | - | 42737:21820-21839 | None:intergenic | 35.0% |
| !!! | TAAGCACTTTTGAGACGATT+TGG | - | 42737:21695-21714 | None:intergenic | 35.0% |
| AAGTGCACCCATTCTACAAA+TGG | + | 42737:22037-22056 | MS.gene30668:CDS | 40.0% | |
| AATAGACGACCCAACTTAGT+TGG | + | 42737:22130-22149 | MS.gene30668:intron | 40.0% | |
| GATGTTACCAGATATCGCTA+TGG | + | 42737:21566-21585 | MS.gene30668:intron | 40.0% | |
| GCTGCATTATCTAGACACTA+AGG | + | 42737:21934-21953 | MS.gene30668:intron | 40.0% | |
| GGTACAATTGGACTAAGTCT+TGG | + | 42737:20831-20850 | MS.gene30668:CDS | 40.0% | |
| TCTACAAATGGTTCGTGAAG+AGG | + | 42737:22049-22068 | MS.gene30668:CDS | 40.0% | |
| TGTCATTGCCATACAAGTGT+CGG | + | 42737:21211-21230 | MS.gene30668:intron | 40.0% | |
| TGTTACCAGATATCGCTATG+GGG | + | 42737:21568-21587 | MS.gene30668:intron | 40.0% | |
| TTTCCATAAGCTCTTCCAGA+TGG | + | 42737:21802-21821 | MS.gene30668:intron | 40.0% | |
| ! | ATTGCAACAAGAGCACATAG+AGG | - | 42737:21963-21982 | None:intergenic | 40.0% |
| ! | CTTTTGCTCCTTCATCCTAA+AGG | - | 42737:21881-21900 | None:intergenic | 40.0% |
| ! | GCATCGACACTTCAAATTGA+AGG | - | 42737:21268-21287 | None:intergenic | 40.0% |
| !!! | GTGGTTCTATGCATTGGTTT+TGG | + | 42737:21313-21332 | MS.gene30668:intron | 40.0% |
| !!! | GTTTTGGCTGATACTCTTCT+GGG | + | 42737:21329-21348 | MS.gene30668:intron | 40.0% |
| !!! | TTGTTTGGTCTTGGTTTTCG+AGG | + | 42737:22229-22248 | MS.gene30668:CDS | 40.0% |
| AGAATGGGTGCACTTCTTCT+AGG | - | 42737:22032-22051 | None:intergenic | 45.0% | |
| AGACCATCTGGAAGAGCTTA+TGG | - | 42737:21808-21827 | None:intergenic | 45.0% | |
| GAATGGGTGCACTTCTTCTA+GGG | - | 42737:22031-22050 | None:intergenic | 45.0% | |
| GATGCTACAATGTGTTCGTG+CGG | + | 42737:21283-21302 | MS.gene30668:intron | 45.0% | |
| TGGTACAGGTAAAGAGTCCA+TGG | - | 42737:21355-21374 | None:intergenic | 45.0% | |
| TTCATCCCCATAGCGATATC+TGG | - | 42737:21576-21595 | None:intergenic | 45.0% | |
| !!! | GGTTTTGGCTGATACTCTTC+TGG | + | 42737:21328-21347 | MS.gene30668:intron | 45.0% |
| ATAGCTGACCATTCGGATCC+CGG | - | 42737:20995-21014 | None:intergenic | 50.0% | |
| ATATTCTCCCGGGATCCGAA+TGG | + | 42737:20984-21003 | MS.gene30668:CDS | 50.0% | |
| CCATGCCAATGATCGCAAGA+GGG | - | 42737:21036-21055 | None:intergenic | 50.0% | |
| GATCATTGGCATGGCACTCA+AGG | + | 42737:21042-21061 | MS.gene30668:CDS | 50.0% | |
| GGTTTCCCTCTTGCGATCAT+TGG | + | 42737:21028-21047 | MS.gene30668:CDS | 50.0% | |
| GTGTTCGTGCGGTTTCTTTG+TGG | + | 42737:21294-21313 | MS.gene30668:intron | 50.0% | |
| ! | CACTGAAGTGTCAGTGGTAC+AGG | - | 42737:21369-21388 | None:intergenic | 50.0% |
| ! | GTTGGACACTGAAGTGTCAG+TGG | - | 42737:21375-21394 | None:intergenic | 50.0% |
| !! | GGTCTTGGTTTTCGAGGCTA+AGG | + | 42737:22235-22254 | MS.gene30668:CDS | 50.0% |
| CCCTCTTGCGATCATTGGCA+TGG | + | 42737:21033-21052 | MS.gene30668:CDS | 55.0% | |
| GCCATGCCAATGATCGCAAG+AGG | - | 42737:21037-21056 | None:intergenic | 55.0% | |
| GCTGATACTCTTCTGGGCCA+TGG | + | 42737:21335-21354 | MS.gene30668:intron | 55.0% | |
| GTCGGTCTCCGACACTTGTA+TGG | - | 42737:21222-21241 | None:intergenic | 55.0% | |
| TAGCTGACCATTCGGATCCC+GGG | - | 42737:20994-21013 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 42737 | gene | 20822 | 22289 | 20822 | ID=MS.gene30668 |
| 42737 | mRNA | 20822 | 22289 | 20822 | ID=MS.gene30668.t1;Parent=MS.gene30668 |
| 42737 | exon | 20822 | 20868 | 20822 | ID=MS.gene30668.t1.exon1;Parent=MS.gene30668.t1 |
| 42737 | CDS | 20822 | 20868 | 20822 | ID=cds.MS.gene30668.t1;Parent=MS.gene30668.t1 |
| 42737 | exon | 20961 | 21063 | 20961 | ID=MS.gene30668.t1.exon2;Parent=MS.gene30668.t1 |
| 42737 | CDS | 20961 | 21063 | 20961 | ID=cds.MS.gene30668.t1;Parent=MS.gene30668.t1 |
| 42737 | exon | 22017 | 22070 | 22017 | ID=MS.gene30668.t1.exon3;Parent=MS.gene30668.t1 |
| 42737 | CDS | 22017 | 22070 | 22017 | ID=cds.MS.gene30668.t1;Parent=MS.gene30668.t1 |
| 42737 | exon | 22209 | 22289 | 22209 | ID=MS.gene30668.t1.exon4;Parent=MS.gene30668.t1 |
| 42737 | CDS | 22209 | 22289 | 22209 | ID=cds.MS.gene30668.t1;Parent=MS.gene30668.t1 |
| Gene Sequence |
| Protein sequence |
