AlfalfaGEDB | MS.gene30706

M. sativa cultivar XinJiangDaYe / MS.gene30706

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene30706.t1	KEH26227.1	86	100	12	1	1	100	1	98	1.00E-38	170.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene30706.t1	A0A072U992	86.0	100	12	1	1	100	1	98	7.4e-39	170.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050026	MS.gene30706	0.807487	4.94E-50	-1.69E-46
MS.gene050750	MS.gene30706	0.81546	9.14E-52	-1.69E-46
MS.gene050953	MS.gene30706	0.82095	5.23E-53	-1.69E-46
MS.gene052867	MS.gene30706	0.831727	1.42E-55	-1.69E-46
MS.gene053166	MS.gene30706	0.803816	2.91E-49	-1.69E-46
MS.gene05415	MS.gene30706	0.826151	3.17E-54	-1.69E-46
MS.gene054160	MS.gene30706	0.864947	8.43E-65	-1.69E-46
MS.gene054291	MS.gene30706	-0.802561	5.29E-49	-1.69E-46
MS.gene054405	MS.gene30706	0.815587	8.57E-52	-1.69E-46
MS.gene055227	MS.gene30706	0.809823	1.56E-50	-1.69E-46
MS.gene055701	MS.gene30706	0.822133	2.79E-53	-1.69E-46
MS.gene055818	MS.gene30706	0.831937	1.26E-55	-1.69E-46
MS.gene056366	MS.gene30706	0.803019	4.26E-49	-1.69E-46
MS.gene056506	MS.gene30706	0.81543	9.28E-52	-1.69E-46
MS.gene058146	MS.gene30706	0.815372	9.56E-52	-1.69E-46
MS.gene058979	MS.gene30706	0.829896	3.98E-55	-1.69E-46
MS.gene059382	MS.gene30706	0.976286	1.55E-141	-1.69E-46
MS.gene060011	MS.gene30706	0.80469	1.91E-49	-1.69E-46
MS.gene060572	MS.gene30706	0.803775	2.97E-49	-1.69E-46
MS.gene060998	MS.gene30706	0.807339	5.30E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene30706.t1	MTR_6g048070	86.000	100	12	1	1	100	1	98	1.01e-53	168
MS.gene30706.t1	MTR_6g013220	39.610	154	65	2	93	218	55	208	1.54e-25	99.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 53 sgRNAs with CRISPR-Local

Find 65 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TAAAATGCTTTGAAAATTTA+TGG	0.103412	6.2:-42373745	None:intergenic
AGGAATTATTATGAATATTT+TGG	0.109615	6.2:+42373187	None:intergenic
ACCAAGAGCCATCTCTTATT+TGG	0.186353	6.2:-42373521	None:intergenic
CCATACGCCCGTTTGTTCTT+AGG	0.263802	6.2:+42373306	MS.gene30706:CDS
CTCACTGAGGAAACGGTTAA+AGG	0.312213	6.2:+42373374	MS.gene30706:CDS
GGATTACTTGGTTTGGAAAC+AGG	0.343584	6.2:+42373225	MS.gene30706:CDS
GAAGACAATTTCAAATCCTT+TGG	0.353154	6.2:+42373652	MS.gene30706:CDS
GTCACACGGATTACTTGGTT+TGG	0.363011	6.2:+42373218	MS.gene30706:CDS
GTTCGGAAATGGTTGGGATC+AGG	0.375886	6.2:+42373770	MS.gene30706:CDS
TTCAAATCCTTTGGTGGTGA+TGG	0.384513	6.2:+42373661	MS.gene30706:CDS
GTAAAGAGAGAGATAATTTA+CGG	0.394352	6.2:+42373609	MS.gene30706:CDS
ACTTGGTTTGGAAACAGGTT+GGG	0.398496	6.2:+42373230	MS.gene30706:CDS
GTCTACAAAATACTCAAAAT+CGG	0.406722	6.2:-42373723	None:intergenic
TGAATGTCACACGGATTACT+TGG	0.411945	6.2:+42373213	MS.gene30706:CDS
GGGTTGATTCGGTTAGGACC+AGG	0.413078	6.2:+42373251	MS.gene30706:CDS
ACCAAATAAGAGATGGCTCT+TGG	0.414703	6.2:+42373520	MS.gene30706:CDS
TCCACCAATCCCATCACAAC+TGG	0.419830	6.2:-42373335	None:intergenic
GAAACAGGTTGGGGTTGATT+CGG	0.433871	6.2:+42373240	MS.gene30706:CDS
TTTAAGGTTCGGAAATGGTT+GGG	0.445226	6.2:+42373764	MS.gene30706:CDS
GGAGTGATTTGCTGAACTTA+AGG	0.461995	6.2:+42373630	MS.gene30706:CDS
GACAATTTCAAATCCTTTGG+TGG	0.464703	6.2:+42373655	MS.gene30706:CDS
GGATTATACCTAAGAACAAA+CGG	0.466483	6.2:-42373314	None:intergenic
AAGAGATGGCTCTTGGTTGA+AGG	0.467671	6.2:+42373527	MS.gene30706:CDS
TCATAACAATGAAGCACTAA+AGG	0.476464	6.2:-42373815	None:intergenic
CATAACAATGAAGCACTAAA+GGG	0.476728	6.2:-42373814	None:intergenic
AGGTTGGGGTTGATTCGGTT+AGG	0.490838	6.2:+42373245	MS.gene30706:CDS
TCAAATCCTTTGGTGGTGAT+GGG	0.493046	6.2:+42373662	MS.gene30706:CDS
AGAAGGAAGACAATTTAAGC+TGG	0.499740	6.2:+42373405	MS.gene30706:CDS
TAGGTATAATCCAGTTGTGA+TGG	0.501054	6.2:+42373325	MS.gene30706:CDS
CTTGGTTTGGAAACAGGTTG+GGG	0.501383	6.2:+42373231	MS.gene30706:CDS
TACTTGGTTTGGAAACAGGT+TGG	0.506523	6.2:+42373229	MS.gene30706:CDS
TTCGGAAATGGTTGGGATCA+GGG	0.508284	6.2:+42373771	MS.gene30706:CDS
TCTCTCTCTTTACGACAACT+TGG	0.508403	6.2:-42373599	None:intergenic
GGGGAAAGTATTACTCAGTC+AGG	0.520760	6.2:+42373272	MS.gene30706:CDS
TTCGAAGCTCACTGAGGAAA+CGG	0.521223	6.2:+42373367	MS.gene30706:CDS
CCTAAGAACAAACGGGCGTA+TGG	0.530528	6.2:-42373306	None:intergenic
GGTTAAAGGATGTTTGAAGA+AGG	0.530922	6.2:+42373388	MS.gene30706:CDS
TCCAGTTGTGATGGGATTGG+TGG	0.539138	6.2:+42373334	MS.gene30706:CDS
AGGTATAATCCAGTTGTGAT+GGG	0.541100	6.2:+42373326	MS.gene30706:CDS
GATTATACCTAAGAACAAAC+GGG	0.541259	6.2:-42373313	None:intergenic
TTCAAAGACAAGAAAAGTGG+GGG	0.560074	6.2:+42373449	MS.gene30706:CDS
GGATCAGGGAGAGTCCGCTC+AGG	0.570190	6.2:+42373785	MS.gene30706:CDS
AACTTACCCATCACCACCAA+AGG	0.574656	6.2:-42373668	None:intergenic
GTTGATTCGGTTAGGACCAG+GGG	0.579094	6.2:+42373253	MS.gene30706:CDS
GGTTGATTCGGTTAGGACCA+GGG	0.584355	6.2:+42373252	MS.gene30706:CDS
GAAGGAAGACAATTTAAGCT+GGG	0.584879	6.2:+42373406	MS.gene30706:CDS
GACTGAGTAATACTTTCCCC+TGG	0.585428	6.2:-42373269	None:intergenic
AAACTTTACCAAATAAGAGA+TGG	0.585590	6.2:+42373513	MS.gene30706:CDS
TAATCCAGTTGTGATGGGAT+TGG	0.599371	6.2:+42373331	MS.gene30706:CDS
TTTCAAAGACAAGAAAAGTG+GGG	0.614665	6.2:+42373448	MS.gene30706:CDS
AACGGGCGTATGGACTGTGA+TGG	0.658353	6.2:-42373296	None:intergenic
CTAAAGGGCGCTGACCTGAG+CGG	0.669360	6.2:-42373799	None:intergenic
AAGACGTTCGAAGCTCACTG+AGG	0.781877	6.2:+42373361	MS.gene30706:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TAAAATGCTTTGAAAATTTA+TGG	-	chr6.2:42373748-42373767	None:intergenic	15.0%
!!!	TAAATTTTCAAAGCATTTTA+AGG	+	chr6.2:42373748-42373767	MS.gene30706:CDS	15.0%
!!!	TTGTCTTTGAAAAATGATTT+TGG	-	chr6.2:42373440-42373459	None:intergenic	20.0%
!	AAACTTTACCAAATAAGAGA+TGG	+	chr6.2:42373513-42373532	MS.gene30706:CDS	25.0%
!	GTAAAGAGAGAGATAATTTA+CGG	+	chr6.2:42373609-42373628	MS.gene30706:CDS	25.0%
!	GTCTACAAAATACTCAAAAT+CGG	-	chr6.2:42373726-42373745	None:intergenic	25.0%
!!	TATTGATGTGTCTTTTCTTT+TGG	+	chr6.2:42373568-42373587	MS.gene30706:CDS	25.0%
!!	TTTTCAAAGACAAGAAAAGT+GGG	+	chr6.2:42373447-42373466	MS.gene30706:CDS	25.0%
!!!	TTTCAAAGCATTTTAAGGTT+CGG	+	chr6.2:42373753-42373772	MS.gene30706:CDS	25.0%
!!!	TTTTTCAAAGACAAGAAAAG+TGG	+	chr6.2:42373446-42373465	MS.gene30706:CDS	25.0%
	GAAGACAATTTCAAATCCTT+TGG	+	chr6.2:42373652-42373671	MS.gene30706:CDS	30.0%
	GATTATACCTAAGAACAAAC+GGG	-	chr6.2:42373316-42373335	None:intergenic	30.0%
	GGATTATACCTAAGAACAAA+CGG	-	chr6.2:42373317-42373336	None:intergenic	30.0%
	TTTCAAAGACAAGAAAAGTG+GGG	+	chr6.2:42373448-42373467	MS.gene30706:CDS	30.0%
!	CATAACAATGAAGCACTAAA+GGG	-	chr6.2:42373817-42373836	None:intergenic	30.0%
!	TCATAACAATGAAGCACTAA+AGG	-	chr6.2:42373818-42373837	None:intergenic	30.0%
!!	AAAATCGGCTTCTTTTTCTT+CGG	-	chr6.2:42373711-42373730	None:intergenic	30.0%
	AGAAGGAAGACAATTTAAGC+TGG	+	chr6.2:42373405-42373424	MS.gene30706:CDS	35.0%
	AGGTATAATCCAGTTGTGAT+GGG	+	chr6.2:42373326-42373345	MS.gene30706:CDS	35.0%
	GAAGGAAGACAATTTAAGCT+GGG	+	chr6.2:42373406-42373425	MS.gene30706:CDS	35.0%
	GACAATTTCAAATCCTTTGG+TGG	+	chr6.2:42373655-42373674	MS.gene30706:CDS	35.0%
	GGTTAAAGGATGTTTGAAGA+AGG	+	chr6.2:42373388-42373407	MS.gene30706:CDS	35.0%
	TAGGTATAATCCAGTTGTGA+TGG	+	chr6.2:42373325-42373344	MS.gene30706:CDS	35.0%
	TTCAAAGACAAGAAAAGTGG+GGG	+	chr6.2:42373449-42373468	MS.gene30706:CDS	35.0%
!	TTTAAGGTTCGGAAATGGTT+GGG	+	chr6.2:42373764-42373783	MS.gene30706:CDS	35.0%
!!!	AGCATTTTAAGGTTCGGAAA+TGG	+	chr6.2:42373759-42373778	MS.gene30706:CDS	35.0%
!!!	GTGATGGGTAAGTTTTATGA+TGG	+	chr6.2:42373677-42373696	MS.gene30706:CDS	35.0%
!!!	TTTTAAGGTTCGGAAATGGT+TGG	+	chr6.2:42373763-42373782	MS.gene30706:CDS	35.0%
!!!	TTTTGGGTTTGAATGTCACA+CGG	+	chr6.2:42373204-42373223	MS.gene30706:CDS	35.0%
	ACCAAATAAGAGATGGCTCT+TGG	+	chr6.2:42373520-42373539	MS.gene30706:CDS	40.0%
	ACCAAGAGCCATCTCTTATT+TGG	-	chr6.2:42373524-42373543	None:intergenic	40.0%
	TCTCTCTCTTTACGACAACT+TGG	-	chr6.2:42373602-42373621	None:intergenic	40.0%
!	TAATCCAGTTGTGATGGGAT+TGG	+	chr6.2:42373331-42373350	MS.gene30706:CDS	40.0%
!	TGAATGTCACACGGATTACT+TGG	+	chr6.2:42373213-42373232	MS.gene30706:CDS	40.0%
!!	ACTTGGTTTGGAAACAGGTT+GGG	+	chr6.2:42373230-42373249	MS.gene30706:CDS	40.0%
!!	GGAGTGATTTGCTGAACTTA+AGG	+	chr6.2:42373630-42373649	MS.gene30706:CDS	40.0%
!!	GGATTACTTGGTTTGGAAAC+AGG	+	chr6.2:42373225-42373244	MS.gene30706:CDS	40.0%
!!	TACTTGGTTTGGAAACAGGT+TGG	+	chr6.2:42373229-42373248	MS.gene30706:CDS	40.0%
!!	TCAAATCCTTTGGTGGTGAT+GGG	+	chr6.2:42373662-42373681	MS.gene30706:CDS	40.0%
!!	TTCAAATCCTTTGGTGGTGA+TGG	+	chr6.2:42373661-42373680	MS.gene30706:CDS	40.0%
	AACTTACCCATCACCACCAA+AGG	-	chr6.2:42373671-42373690	None:intergenic	45.0%
	AAGAGATGGCTCTTGGTTGA+AGG	+	chr6.2:42373527-42373546	MS.gene30706:CDS	45.0%
	CTCACTGAGGAAACGGTTAA+AGG	+	chr6.2:42373374-42373393	MS.gene30706:CDS	45.0%
	GACTGAGTAATACTTTCCCC+TGG	-	chr6.2:42373272-42373291	None:intergenic	45.0%
	TTCGAAGCTCACTGAGGAAA+CGG	+	chr6.2:42373367-42373386	MS.gene30706:CDS	45.0%
	TTCGGAAATGGTTGGGATCA+GGG	+	chr6.2:42373771-42373790	MS.gene30706:CDS	45.0%
!	GAAACAGGTTGGGGTTGATT+CGG	+	chr6.2:42373240-42373259	MS.gene30706:CDS	45.0%
!	GTCACACGGATTACTTGGTT+TGG	+	chr6.2:42373218-42373237	MS.gene30706:CDS	45.0%
!	TCGGCTTCTTTTTCTTCGGA+TGG	-	chr6.2:42373707-42373726	None:intergenic	45.0%
!!	CTTGGTTTGGAAACAGGTTG+GGG	+	chr6.2:42373231-42373250	MS.gene30706:CDS	45.0%
!!	GGGGAAAGTATTACTCAGTC+AGG	+	chr6.2:42373272-42373291	MS.gene30706:CDS	45.0%
	AAGACGTTCGAAGCTCACTG+AGG	+	chr6.2:42373361-42373380	MS.gene30706:CDS	50.0%
	CCATACGCCCGTTTGTTCTT+AGG	+	chr6.2:42373306-42373325	MS.gene30706:CDS	50.0%
	CCTAAGAACAAACGGGCGTA+TGG	-	chr6.2:42373309-42373328	None:intergenic	50.0%
	GTTCGGAAATGGTTGGGATC+AGG	+	chr6.2:42373770-42373789	MS.gene30706:CDS	50.0%
	TCCACCAATCCCATCACAAC+TGG	-	chr6.2:42373338-42373357	None:intergenic	50.0%
!	TCCAGTTGTGATGGGATTGG+TGG	+	chr6.2:42373334-42373353	MS.gene30706:CDS	50.0%
!!	AGGTTGGGGTTGATTCGGTT+AGG	+	chr6.2:42373245-42373264	MS.gene30706:CDS	50.0%
!!	GGTTGATTCGGTTAGGACCA+GGG	+	chr6.2:42373252-42373271	MS.gene30706:CDS	50.0%
!!	GTTGATTCGGTTAGGACCAG+GGG	+	chr6.2:42373253-42373272	MS.gene30706:CDS	50.0%
!	AACGGGCGTATGGACTGTGA+TGG	-	chr6.2:42373299-42373318	None:intergenic	55.0%
!!	GGGTTGATTCGGTTAGGACC+AGG	+	chr6.2:42373251-42373270	MS.gene30706:CDS	55.0%
	GTGGGGGAATCATGTTCGCG+TGG	+	chr6.2:42373465-42373484	MS.gene30706:CDS	60.0%
!!	CTAAAGGGCGCTGACCTGAG+CGG	-	chr6.2:42373802-42373821	None:intergenic	60.0%
	GGATCAGGGAGAGTCCGCTC+AGG	+	chr6.2:42373785-42373804	MS.gene30706:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr6.2	gene	42373197	42373853	42373197	ID=MS.gene30706
chr6.2	mRNA	42373197	42373853	42373197	ID=MS.gene30706.t1;Parent=MS.gene30706
chr6.2	exon	42373197	42373853	42373197	ID=MS.gene30706.t1.exon1;Parent=MS.gene30706.t1
chr6.2	CDS	42373197	42373853	42373197	ID=cds.MS.gene30706.t1;Parent=MS.gene30706.t1

Gene Sequence

>MS.gene30706

ATGAATATTTTGGGTTTGAATGTCACACGGATTACTTGGTTTGGAAACAGGTTGGGGTTGATTCGGTTAGGACCAGGGGAAAGTATTACTCAGTCAGGACCATCACAGTCCATACGCCCGTTTGTTCTTAGGTATAATCCAGTTGTGATGGGATTGGTGGATACAAGACGTTCGAAGCTCACTGAGGAAACGGTTAAAGGATGTTTGAAGAAGGAAGACAATTTAAGCTGGGTAGATGTTCCAAAATCATTTTTCAAAGACAAGAAAAGTGGGGGAATCATGTTCGCGTGGAATGAACTTTATTTTACGATATCAAAAACTTTACCAAATAAGAGATGGCTCTTGGTTGAAGGTAATTTGATAAAAGAACGTATTGATGTGTCTTTTCTTTTGGTTTCTGAACCAAGTTGTCGTAAAGAGAGAGATAATTTACGGAGTGATTTGCTGAACTTAAGGAAGACAATTTCAAATCCTTTGGTGGTGATGGGTAAGTTTTATGATGGTGTACCATCCGAAGAAAAAGAAGCCGATTTTGAGTATTTTGTAGACCATAAATTTTCAAAGCATTTTAAGGTTCGGAAATGGTTGGGATCAGGGAGAGTCCGCTCAGGTCAGCGCCCTTTAGTGCTTCATTGTTATGAACAAGATGATATTTGA

Protein sequence

>MS.gene30706.t1

MNILGLNVTRITWFGNRLGLIRLGPGESITQSGPSQSIRPFVLRYNPVVMGLVDTRRSKLTEETVKGCLKKEDNLSWVDVPKSFFKDKKSGGIMFAWNELYFTISKTLPNKRWLLVEGNLIKERIDVSFLLVSEPSCRKERDNLRSDLLNLRKTISNPLVVMGKFYDGVPSEEKEADFEYFVDHKFSKHFKVRKWLGSGRVRSGQRPLVLHCYEQDDI