AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene054160


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene054160.t1 MTR_6g048070 88.000 100 10 1 1 100 1 98 1.61e-55 172
MS.gene054160.t1 MTR_6g013220 39.610 154 65 2 93 218 55 208 4.90e-26 100
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 51 sgRNAs with CRISPR-Local

Find 64 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAAAATGCTTTGAAAATTTA+TGG 0.103412 6.1:+27928942 None:intergenic
ACCAAGAGCCATCTCTTATT+TGG 0.186353 6.1:+27929166 None:intergenic
CAATACGCCCGTTTGTTCTT+AGG 0.226667 6.1:-27929381 MS.gene054160:CDS
CTCACTGAGGAAACGGTTAA+AGG 0.312213 6.1:-27929313 MS.gene054160:CDS
GAAGACAATTTCAAATCCTT+TGG 0.353154 6.1:-27929035 MS.gene054160:CDS
AGATAATTTACAGAGTGATT+TGG 0.366621 6.1:-27929068 MS.gene054160:CDS
GGATTACTCGGTTTGGAAAC+AGG 0.367768 6.1:-27929462 MS.gene054160:CDS
GTTCGGAAATGGTTGGGATC+AGG 0.375886 6.1:-27928917 MS.gene054160:CDS
TTCAAATCCTTTGGTGGTGA+TGG 0.384513 6.1:-27929026 MS.gene054160:CDS
ACTCGGTTTGGAAACAGGTT+GGG 0.406351 6.1:-27929457 MS.gene054160:CDS
GTCTACAAAATACTCAAAAT+CGG 0.406722 6.1:+27928964 None:intergenic
GGGTTGATTCGGTTAGGACC+AGG 0.413078 6.1:-27929436 MS.gene054160:CDS
ACCAAATAAGAGATGGCTCT+TGG 0.414703 6.1:-27929167 MS.gene054160:CDS
TCCACCAATCCCATCACAAC+TGG 0.419830 6.1:+27929352 None:intergenic
GAAACAGGTTGGGGTTGATT+CGG 0.433871 6.1:-27929447 MS.gene054160:CDS
GTCACACGGATTACTCGGTT+TGG 0.436680 6.1:-27929469 MS.gene054160:CDS
TTTAAGGTTCGGAAATGGTT+GGG 0.445226 6.1:-27928923 MS.gene054160:CDS
AGAGTGATTTGGTGAACTTA+AGG 0.455039 6.1:-27929057 MS.gene054160:CDS
GACAATTTCAAATCCTTTGG+TGG 0.464703 6.1:-27929032 MS.gene054160:CDS
GGATTATACCTAAGAACAAA+CGG 0.466483 6.1:+27929373 None:intergenic
AAGAGATGGCTCTTGGTTGA+AGG 0.467671 6.1:-27929160 MS.gene054160:CDS
CTCGGTTTGGAAACAGGTTG+GGG 0.473109 6.1:-27929456 MS.gene054160:CDS
TCATTACAATGAAGCACTAA+AGG 0.481197 6.1:+27928872 None:intergenic
TACTCGGTTTGGAAACAGGT+TGG 0.489557 6.1:-27929458 MS.gene054160:CDS
AGGTTGGGGTTGATTCGGTT+AGG 0.490838 6.1:-27929442 MS.gene054160:CDS
TCAAATCCTTTGGTGGTGAT+GGG 0.493046 6.1:-27929025 MS.gene054160:CDS
AGAAGGAAGACAATTTAAGC+TGG 0.499740 6.1:-27929282 MS.gene054160:CDS
CATTACAATGAAGCACTAAA+GGG 0.500328 6.1:+27928873 None:intergenic
TAGGTATAATCCAGTTGTGA+TGG 0.501054 6.1:-27929362 MS.gene054160:CDS
TTCGGAAATGGTTGGGATCA+GGG 0.508284 6.1:-27928916 MS.gene054160:CDS
TCTCTCTCTTTACGACAACT+TGG 0.508403 6.1:+27929088 None:intergenic
GGGGAAAGTATTACTCAGTC+AGG 0.520760 6.1:-27929415 MS.gene054160:CDS
TTCGAAGCTCACTGAGGAAA+CGG 0.521223 6.1:-27929320 MS.gene054160:CDS
TGAATGTCACACGGATTACT+CGG 0.522233 6.1:-27929474 MS.gene054160:CDS
GGTTAAAGGATGTTTGAAGA+AGG 0.530922 6.1:-27929299 MS.gene054160:CDS
TCCAGTTGTGATGGGATTGG+TGG 0.539138 6.1:-27929353 MS.gene054160:CDS
AGGTATAATCCAGTTGTGAT+GGG 0.541100 6.1:-27929361 MS.gene054160:CDS
GATTATACCTAAGAACAAAC+GGG 0.541259 6.1:+27929374 None:intergenic
TTCAAAGACAAGAAAAGTGG+GGG 0.560074 6.1:-27929238 MS.gene054160:CDS
GGATCAGGGAGAGTCCGCTC+AGG 0.570190 6.1:-27928902 MS.gene054160:CDS
AACTTACCCATCACCACCAA+AGG 0.574656 6.1:+27929019 None:intergenic
GTTGATTCGGTTAGGACCAG+GGG 0.579094 6.1:-27929434 MS.gene054160:CDS
GGTTGATTCGGTTAGGACCA+GGG 0.584355 6.1:-27929435 MS.gene054160:CDS
GAAGGAAGACAATTTAAGCT+GGG 0.584879 6.1:-27929281 MS.gene054160:CDS
GACTGAGTAATACTTTCCCC+TGG 0.585428 6.1:+27929418 None:intergenic
AAACTTTACCAAATAAGAGA+TGG 0.585590 6.1:-27929174 MS.gene054160:CDS
TAATCCAGTTGTGATGGGAT+TGG 0.599371 6.1:-27929356 MS.gene054160:CDS
TTTCAAAGACAAGAAAAGTG+GGG 0.614665 6.1:-27929239 MS.gene054160:CDS
AACGGGCGTATTGACTCTGA+TGG 0.630782 6.1:+27929391 None:intergenic
CTAAAGGGCGCTGACCTGAG+CGG 0.669360 6.1:+27928888 None:intergenic
AAGACGTTCGAAGCTCACTG+AGG 0.781877 6.1:-27929326 MS.gene054160:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAAAATGCTTTGAAAATTTA+TGG + chr6.1:27929407-27929426 None:intergenic 15.0%
!!! TAAATTTTCAAAGCATTTTA+AGG - chr6.1:27929407-27929426 MS.gene054160:CDS 15.0%
!!! TTGTCTTTGAAAAATGATTT+TGG + chr6.1:27929099-27929118 None:intergenic 20.0%
! AAACTTTACCAAATAAGAGA+TGG - chr6.1:27929172-27929191 MS.gene054160:CDS 25.0%
! AGATAATTTACAGAGTGATT+TGG - chr6.1:27929278-27929297 MS.gene054160:CDS 25.0%
! GTCTACAAAATACTCAAAAT+CGG + chr6.1:27929385-27929404 None:intergenic 25.0%
!! TATTGATGTGTCTTTTCTTT+TGG - chr6.1:27929227-27929246 MS.gene054160:CDS 25.0%
!! TTTTCAAAGACAAGAAAAGT+GGG - chr6.1:27929106-27929125 MS.gene054160:CDS 25.0%
!!! TTTCAAAGCATTTTAAGGTT+CGG - chr6.1:27929412-27929431 MS.gene054160:CDS 25.0%
!!! TTTTTCAAAGACAAGAAAAG+TGG - chr6.1:27929105-27929124 MS.gene054160:CDS 25.0%
GAAGACAATTTCAAATCCTT+TGG - chr6.1:27929311-27929330 MS.gene054160:CDS 30.0%
GATTATACCTAAGAACAAAC+GGG + chr6.1:27928975-27928994 None:intergenic 30.0%
GGATTATACCTAAGAACAAA+CGG + chr6.1:27928976-27928995 None:intergenic 30.0%
TTTCAAAGACAAGAAAAGTG+GGG - chr6.1:27929107-27929126 MS.gene054160:CDS 30.0%
! CATTACAATGAAGCACTAAA+GGG + chr6.1:27929476-27929495 None:intergenic 30.0%
! TCATTACAATGAAGCACTAA+AGG + chr6.1:27929477-27929496 None:intergenic 30.0%
!! AAAATCGGCTTCTTTTTCTT+CGG + chr6.1:27929370-27929389 None:intergenic 30.0%
!!! TTTTAGGTTTGAATGTCACA+CGG - chr6.1:27928863-27928882 MS.gene054160:CDS 30.0%
AGAAGGAAGACAATTTAAGC+TGG - chr6.1:27929064-27929083 MS.gene054160:CDS 35.0%
AGGTATAATCCAGTTGTGAT+GGG - chr6.1:27928985-27929004 MS.gene054160:CDS 35.0%
GAAGGAAGACAATTTAAGCT+GGG - chr6.1:27929065-27929084 MS.gene054160:CDS 35.0%
GACAATTTCAAATCCTTTGG+TGG - chr6.1:27929314-27929333 MS.gene054160:CDS 35.0%
GGTTAAAGGATGTTTGAAGA+AGG - chr6.1:27929047-27929066 MS.gene054160:CDS 35.0%
TAGGTATAATCCAGTTGTGA+TGG - chr6.1:27928984-27929003 MS.gene054160:CDS 35.0%
TTCAAAGACAAGAAAAGTGG+GGG - chr6.1:27929108-27929127 MS.gene054160:CDS 35.0%
! TTTAAGGTTCGGAAATGGTT+GGG - chr6.1:27929423-27929442 MS.gene054160:CDS 35.0%
!! AGAGTGATTTGGTGAACTTA+AGG - chr6.1:27929289-27929308 MS.gene054160:CDS 35.0%
!!! AGCATTTTAAGGTTCGGAAA+TGG - chr6.1:27929418-27929437 MS.gene054160:CDS 35.0%
!!! GTGATGGGTAAGTTTTATGA+TGG - chr6.1:27929336-27929355 MS.gene054160:CDS 35.0%
!!! TTTTAAGGTTCGGAAATGGT+TGG - chr6.1:27929422-27929441 MS.gene054160:CDS 35.0%
ACCAAATAAGAGATGGCTCT+TGG - chr6.1:27929179-27929198 MS.gene054160:CDS 40.0%
ACCAAGAGCCATCTCTTATT+TGG + chr6.1:27929183-27929202 None:intergenic 40.0%
TCTCTCTCTTTACGACAACT+TGG + chr6.1:27929261-27929280 None:intergenic 40.0%
! TAATCCAGTTGTGATGGGAT+TGG - chr6.1:27928990-27929009 MS.gene054160:CDS 40.0%
! TGAATGTCACACGGATTACT+CGG - chr6.1:27928872-27928891 MS.gene054160:CDS 40.0%
!! TCAAATCCTTTGGTGGTGAT+GGG - chr6.1:27929321-27929340 MS.gene054160:CDS 40.0%
!! TTCAAATCCTTTGGTGGTGA+TGG - chr6.1:27929320-27929339 MS.gene054160:CDS 40.0%
AACTTACCCATCACCACCAA+AGG + chr6.1:27929330-27929349 None:intergenic 45.0%
AAGAGATGGCTCTTGGTTGA+AGG - chr6.1:27929186-27929205 MS.gene054160:CDS 45.0%
CAATACGCCCGTTTGTTCTT+AGG - chr6.1:27928965-27928984 MS.gene054160:CDS 45.0%
CTCACTGAGGAAACGGTTAA+AGG - chr6.1:27929033-27929052 MS.gene054160:CDS 45.0%
GACTGAGTAATACTTTCCCC+TGG + chr6.1:27928931-27928950 None:intergenic 45.0%
TTCGAAGCTCACTGAGGAAA+CGG - chr6.1:27929026-27929045 MS.gene054160:CDS 45.0%
TTCGGAAATGGTTGGGATCA+GGG - chr6.1:27929430-27929449 MS.gene054160:CDS 45.0%
! GAAACAGGTTGGGGTTGATT+CGG - chr6.1:27928899-27928918 MS.gene054160:CDS 45.0%
! TCGGCTTCTTTTTCTTCGGA+TGG + chr6.1:27929366-27929385 None:intergenic 45.0%
!! ACTCGGTTTGGAAACAGGTT+GGG - chr6.1:27928889-27928908 MS.gene054160:CDS 45.0%
!! GGATTACTCGGTTTGGAAAC+AGG - chr6.1:27928884-27928903 MS.gene054160:CDS 45.0%
!! GGGGAAAGTATTACTCAGTC+AGG - chr6.1:27928931-27928950 MS.gene054160:CDS 45.0%
!! TACTCGGTTTGGAAACAGGT+TGG - chr6.1:27928888-27928907 MS.gene054160:CDS 45.0%
AAGACGTTCGAAGCTCACTG+AGG - chr6.1:27929020-27929039 MS.gene054160:CDS 50.0%
GTTCGGAAATGGTTGGGATC+AGG - chr6.1:27929429-27929448 MS.gene054160:CDS 50.0%
TCCACCAATCCCATCACAAC+TGG + chr6.1:27928997-27929016 None:intergenic 50.0%
! GTCACACGGATTACTCGGTT+TGG - chr6.1:27928877-27928896 MS.gene054160:CDS 50.0%
! TCCAGTTGTGATGGGATTGG+TGG - chr6.1:27928993-27929012 MS.gene054160:CDS 50.0%
!! AACGGGCGTATTGACTCTGA+TGG + chr6.1:27928958-27928977 None:intergenic 50.0%
!! AGGTTGGGGTTGATTCGGTT+AGG - chr6.1:27928904-27928923 MS.gene054160:CDS 50.0%
!! CTCGGTTTGGAAACAGGTTG+GGG - chr6.1:27928890-27928909 MS.gene054160:CDS 50.0%
!! GGTTGATTCGGTTAGGACCA+GGG - chr6.1:27928911-27928930 MS.gene054160:CDS 50.0%
!! GTTGATTCGGTTAGGACCAG+GGG - chr6.1:27928912-27928931 MS.gene054160:CDS 50.0%
!! GGGTTGATTCGGTTAGGACC+AGG - chr6.1:27928910-27928929 MS.gene054160:CDS 55.0%
GTGGGGGAATCATGTTCGCG+TGG - chr6.1:27929124-27929143 MS.gene054160:CDS 60.0%
!! CTAAAGGGCGCTGACCTGAG+CGG + chr6.1:27929461-27929480 None:intergenic 60.0%
GGATCAGGGAGAGTCCGCTC+AGG - chr6.1:27929444-27929463 MS.gene054160:CDS 65.0%


Chromosome Type Strat End Strand Name
chr6.1 gene 27928856 27929512 27928856 ID=MS.gene054160
chr6.1 mRNA 27928856 27929512 27928856 ID=MS.gene054160.t1;Parent=MS.gene054160
chr6.1 exon 27928856 27929512 27928856 ID=MS.gene054160.t1.exon1;Parent=MS.gene054160.t1
chr6.1 CDS 27928856 27929512 27928856 ID=cds.MS.gene054160.t1;Parent=MS.gene054160.t1
Gene Sequence

>MS.gene054160

ATGAATATTTTAGGTTTGAATGTCACACGGATTACTCGGTTTGGAAACAGGTTGGGGTTGATTCGGTTAGGACCAGGGGAAAGTATTACTCAGTCAGGACCATCAGAGTCAATACGCCCGTTTGTTCTTAGGTATAATCCAGTTGTGATGGGATTGGTGGATACAAGACGTTCGAAGCTCACTGAGGAAACGGTTAAAGGATGTTTGAAGAAGGAAGACAATTTAAGCTGGGTAGATGTTCCAAAATCATTTTTCAAAGACAAGAAAAGTGGGGGAATCATGTTCGCGTGGAATGAACTTTATTTTACGATATCAAAAACTTTACCAAATAAGAGATGGCTCTTGGTTGAAGGTAATCTGATAAAAGAACGTATTGATGTGTCTTTTCTTTTGGTTTCTGAACCAAGTTGTCGTAAAGAGAGAGATAATTTACAGAGTGATTTGGTGAACTTAAGGAAGACAATTTCAAATCCTTTGGTGGTGATGGGTAAGTTTTATGATGGTGTACCATCCGAAGAAAAAGAAGCCGATTTTGAGTATTTTGTAGACCATAAATTTTCAAAGCATTTTAAGGTTCGGAAATGGTTGGGATCAGGGAGAGTCCGCTCAGGTCAGCGCCCTTTAGTGCTTCATTGTAATGAACAAGATGATATTTGA

Protein sequence

>MS.gene054160.t1

MNILGLNVTRITRFGNRLGLIRLGPGESITQSGPSESIRPFVLRYNPVVMGLVDTRRSKLTEETVKGCLKKEDNLSWVDVPKSFFKDKKSGGIMFAWNELYFTISKTLPNKRWLLVEGNLIKERIDVSFLLVSEPSCRKERDNLQSDLVNLRKTISNPLVVMGKFYDGVPSEEKEADFEYFVDHKFSKHFKVRKWLGSGRVRSGQRPLVLHCNEQDDI