AlfalfaGEDB | MS.gene05623

M. sativa cultivar XinJiangDaYe / MS.gene05623

KEGG: K11254
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005634 GO:0032991 GO:0032993 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:0110165 GO:1901363
PFAM: PF15511.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene05623.t1	KCW52694.1	100	103	0	0	1	103	4	106	7.50E-48	199.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene05623.t1	P62785	100.0	103	0	0	1	103	1	103	1.8e-50	199.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene05623.t1	M5VRN0	100.0	103	0	0	1	103	1	103	5.4e-48	199.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene05623	MS.gene31169	0.807519	4.86E-50	-1.69E-46
MS.gene05623	MS.gene41203	0.805357	1.39E-49	-1.69E-46
MS.gene05623	MS.gene45478	0.807109	5.94E-50	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene04433	MS.gene05623	PPI
MS.gene04452	MS.gene05623	PPI
MS.gene035787	MS.gene05623	PPI
MS.gene05623	MS.gene035897	PPI
MS.gene89487	MS.gene05623	PPI
MS.gene035719	MS.gene05623	PPI
MS.gene05623	MS.gene05608	PPI
MS.gene58854	MS.gene05623	PPI
MS.gene70955	MS.gene05623	PPI
MS.gene040383	MS.gene05623	PPI
MS.gene070242	MS.gene05623	PPI
MS.gene072493	MS.gene05623	PPI
MS.gene30920	MS.gene05623	PPI
MS.gene048746	MS.gene05623	PPI
MS.gene010220	MS.gene05623	PPI
MS.gene08053	MS.gene05623	PPI
MS.gene05623	MS.gene84536	PPI
MS.gene061872	MS.gene05623	PPI
MS.gene05623	MS.gene82546	PPI
MS.gene88680	MS.gene05623	PPI
MS.gene05623	MS.gene60093	PPI
MS.gene070241	MS.gene05623	PPI
MS.gene05623	MS.gene08122	PPI
MS.gene022946	MS.gene05623	PPI
MS.gene05623	MS.gene09873	PPI
MS.gene061876	MS.gene05623	PPI
MS.gene05623	MS.gene04972	PPI
MS.gene012391	MS.gene05623	PPI
MS.gene05623	MS.gene004121	PPI
MS.gene05623	MS.gene62520	PPI
MS.gene09871	MS.gene05623	PPI
MS.gene035897	MS.gene05623	PPI
MS.gene64920	MS.gene05623	PPI
MS.gene05623	MS.gene018578	PPI
MS.gene060463	MS.gene05623	PPI
MS.gene05623	MS.gene04064	PPI
MS.gene05623	MS.gene018580	PPI
MS.gene05623	MS.gene004124	PPI
MS.gene04392	MS.gene05623	PPI
MS.gene05623	MS.gene04052	PPI
MS.gene05623	MS.gene061866	PPI
MS.gene04068	MS.gene05623	PPI
MS.gene05623	MS.gene64650	PPI
MS.gene05623	MS.gene79195	PPI
MS.gene05623	MS.gene048705	PPI
MS.gene04434	MS.gene05623	PPI
MS.gene05623	MS.gene061874	PPI
MS.gene05623	MS.gene04576	PPI
MS.gene05623	MS.gene061869	PPI
MS.gene08121	MS.gene05623	PPI
MS.gene022932	MS.gene05623	PPI
MS.gene88681	MS.gene05623	PPI
MS.gene070244	MS.gene05623	PPI
MS.gene035896	MS.gene05623	PPI
MS.gene05623	MS.gene061867	PPI
MS.gene05623	MS.gene008276	PPI
MS.gene009027	MS.gene05623	PPI
MS.gene31196	MS.gene05623	PPI
MS.gene05623	MS.gene05619	PPI
MS.gene05623	MS.gene73136	PPI
MS.gene05623	MS.gene049821	PPI
MS.gene060465	MS.gene05623	PPI
MS.gene05623	MS.gene04068	PPI
MS.gene043967	MS.gene05623	PPI
MS.gene05623	MS.gene89488	PPI
MS.gene028629	MS.gene05623	PPI
MS.gene05623	MS.gene035720	PPI
MS.gene05623	MS.gene61017	PPI
MS.gene05623	MS.gene09871	PPI
MS.gene05623	MS.gene012390	PPI
MS.gene04067	MS.gene05623	PPI
MS.gene71215	MS.gene05623	PPI
MS.gene05623	MS.gene049705	PPI
MS.gene061870	MS.gene05623	PPI
MS.gene05623	MS.gene04434	PPI
MS.gene05623	MS.gene070907	PPI
MS.gene05623	MS.gene04392	PPI
MS.gene035718	MS.gene05623	PPI
MS.gene05623	MS.gene010219	PPI
MS.gene05623	MS.gene68545	PPI
MS.gene66225	MS.gene05623	PPI
MS.gene071136	MS.gene05623	PPI
MS.gene05623	MS.gene016624	PPI
MS.gene78578	MS.gene05623	PPI
MS.gene79195	MS.gene05623	PPI
MS.gene035721	MS.gene05623	PPI
MS.gene05623	MS.gene048746	PPI
MS.gene056215	MS.gene05623	PPI
MS.gene064557	MS.gene05623	PPI
MS.gene038469	MS.gene05623	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene05623.t1	MTR_7g029510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene05623.t1	MTR_7g099610	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene05623.t1	MTR_5g063620	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene05623.t1	MTR_4g061827	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene05623.t1	MTR_4g061920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene05623.t1	MTR_3g462400	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene05623.t1	MTR_3g054380	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene05623.t1	MTR_3g462730	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene05623.t1	MTR_3g462510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene05623.t1	MTR_3g070920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene05623.t1	MTR_3g061440	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene05623.t1	MTR_3g462410	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene05623.t1	MTR_2g096100	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene05623.t1	MTR_8g038460	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene05623.t1	MTR_4g128150	100.000	102	0	1	102	1	102	1.67e-66	204
MS.gene05623.t1	MTR_7g029520	84.000	50	8	27	76	1	50	2.15e-21	87.0

Query id	Subject id	identity %	alignment length	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene05623.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene05623.t1	AT5G59690	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene05623.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene05623.t1	AT3G46320	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene05623.t1	AT3G45930	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene05623.t1	AT3G53730	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene05623.t1	AT2G28740	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene05623.t1	AT1G07660	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene05623.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene05623.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene05623.t1	AT1G07660	83.495	103	1	1	103	1	86	4.34e-51	155

Find 37 sgRNAs with CRISPR-Local

Find 33 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTTGCCAATCGACGAATTGC+AGG	0.323252	3.2:+53421426	None:intergenic
CAATCGACGAATTGCAGGTT+TGG	0.356355	3.2:+53421431	None:intergenic
GGAAGAACCCTCTATGGATT+TGG	0.365325	3.2:-53421240	MS.gene05623:CDS
AGGTGTCTTGAAGATCTTCT+TGG	0.405705	3.2:-53421355	MS.gene05623:CDS
AGGCAGGGAAGAACCCTCTA+TGG	0.410062	3.2:-53421246	MS.gene05623:CDS
TTCAACCTCCAAATCCATAG+AGG	0.435462	3.2:+53421232	None:intergenic
GGGAGGTAAGGGACTTGGAA+AGG	0.449159	3.2:-53421505	MS.gene05623:CDS
GGAGGTAAGGGACTTGGAAA+GGG	0.467307	3.2:-53421504	MS.gene05623:CDS
GGAAAGGGAGGTAAGGGACT+TGG	0.470633	3.2:-53421510	MS.gene05623:CDS
TGTCTATGCTTTGAAGAGGC+AGG	0.485721	3.2:-53421262	MS.gene05623:CDS
AGGCCGTGGAAAGGGAGGTA+AGG	0.492819	3.2:-53421517	MS.gene05623:CDS
AGAACCCTCTATGGATTTGG+AGG	0.508817	3.2:-53421237	MS.gene05623:CDS
CTCGCGAAAATGTCAGGCCG+TGG	0.510433	3.2:-53421531	None:intergenic
GAAGATCTTCAAGACACCTC+TGG	0.510902	3.2:+53421359	None:intergenic
AAAATGTCAGGCCGTGGAAA+GGG	0.516096	3.2:-53421525	MS.gene05623:CDS
TTACATACACTGAGCATGCT+AGG	0.520723	3.2:-53421311	MS.gene05623:CDS
GGTTCTTCGTGATAACATCC+AGG	0.522451	3.2:-53421460	MS.gene05623:CDS
CAAACCTGCAATTCGTCGAT+TGG	0.529230	3.2:-53421430	MS.gene05623:CDS
AGGTGCAAAGAGGCATAGAA+AGG	0.530485	3.2:-53421481	MS.gene05623:CDS
GTCTATGCTTTGAAGAGGCA+GGG	0.532756	3.2:-53421261	MS.gene05623:CDS
GGCCGTGGAAAGGGAGGTAA+GGG	0.552535	3.2:-53421516	MS.gene05623:CDS
GTCCCTTACCTCCCTTTCCA+CGG	0.555891	3.2:+53421514	None:intergenic
GAAAATGTCAGGCCGTGGAA+AGG	0.558967	3.2:-53421526	None:intergenic
GGTAAGGGACTTGGAAAGGG+AGG	0.560743	3.2:-53421501	MS.gene05623:CDS
GAGGAAGACTGTGACAGCCA+TGG	0.570644	3.2:-53421289	MS.gene05623:CDS
CGTCGATTGGCAAGAAGAGG+TGG	0.589209	3.2:-53421417	MS.gene05623:CDS
TCAACCTCCAAATCCATAGA+GGG	0.597347	3.2:+53421233	None:intergenic
GGTGGTGTCAAGAGAATCAG+TGG	0.607763	3.2:-53421399	MS.gene05623:CDS
TTGCAGGTTTGGTGATACCC+TGG	0.610800	3.2:+53421442	None:intergenic
ATTCGTCGATTGGCAAGAAG+AGG	0.624691	3.2:-53421420	MS.gene05623:CDS
CAAAGCATAGACAACATCCA+TGG	0.629842	3.2:+53421272	None:intergenic
GTTCTTCGTGATAACATCCA+GGG	0.632029	3.2:-53421459	MS.gene05623:CDS
ATGTCAGGCCGTGGAAAGGG+AGG	0.634999	3.2:-53421522	MS.gene05623:CDS
TTGGAAAGGGAGGTGCAAAG+AGG	0.683014	3.2:-53421491	MS.gene05623:CDS
TTGATATATGAAGAAACCAG+AGG	0.683025	3.2:-53421375	MS.gene05623:CDS
ATGTTGTCTATGCTTTGAAG+AGG	0.684273	3.2:-53421266	MS.gene05623:CDS
CATACACTGAGCATGCTAGG+AGG	0.707859	3.2:-53421308	MS.gene05623:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	TTGATATATGAAGAAACCAG+AGG	-	chr3.2:53421380-53421399	MS.gene05623:CDS	30.0%
!!	ATGTTGTCTATGCTTTGAAG+AGG	-	chr3.2:53421489-53421508	MS.gene05623:CDS	35.0%
	AGGTGTCTTGAAGATCTTCT+TGG	-	chr3.2:53421400-53421419	MS.gene05623:CDS	40.0%
	CAAAGCATAGACAACATCCA+TGG	+	chr3.2:53421486-53421505	None:intergenic	40.0%
	GTTCTTCGTGATAACATCCA+GGG	-	chr3.2:53421296-53421315	MS.gene05623:CDS	40.0%
	TCAACCTCCAAATCCATAGA+GGG	+	chr3.2:53421525-53421544	None:intergenic	40.0%
	TTACATACACTGAGCATGCT+AGG	-	chr3.2:53421444-53421463	MS.gene05623:CDS	40.0%
	AGAACCCTCTATGGATTTGG+AGG	-	chr3.2:53421518-53421537	MS.gene05623:CDS	45.0%
	CAAACCTGCAATTCGTCGAT+TGG	-	chr3.2:53421325-53421344	MS.gene05623:CDS	45.0%
	CAATCGACGAATTGCAGGTT+TGG	+	chr3.2:53421327-53421346	None:intergenic	45.0%
	GAAGATCTTCAAGACACCTC+TGG	+	chr3.2:53421399-53421418	None:intergenic	45.0%
	GGAAGAACCCTCTATGGATT+TGG	-	chr3.2:53421515-53421534	MS.gene05623:CDS	45.0%
	GGTTCTTCGTGATAACATCC+AGG	-	chr3.2:53421295-53421314	MS.gene05623:CDS	45.0%
!	AGGTGCAAAGAGGCATAGAA+AGG	-	chr3.2:53421274-53421293	MS.gene05623:CDS	45.0%
!	ATTCGTCGATTGGCAAGAAG+AGG	-	chr3.2:53421335-53421354	MS.gene05623:CDS	45.0%
!!	GTCTATGCTTTGAAGAGGCA+GGG	-	chr3.2:53421494-53421513	MS.gene05623:CDS	45.0%
!!	TGTCTATGCTTTGAAGAGGC+AGG	-	chr3.2:53421493-53421512	MS.gene05623:CDS	45.0%
	CATACACTGAGCATGCTAGG+AGG	-	chr3.2:53421447-53421466	MS.gene05623:CDS	50.0%
	CTTGCCAATCGACGAATTGC+AGG	+	chr3.2:53421332-53421351	None:intergenic	50.0%
	GGAGGTAAGGGACTTGGAAA+GGG	-	chr3.2:53421251-53421270	MS.gene05623:CDS	50.0%
!	GGTGGTGTCAAGAGAATCAG+TGG	-	chr3.2:53421356-53421375	MS.gene05623:CDS	50.0%
!	TTGGAAAGGGAGGTGCAAAG+AGG	-	chr3.2:53421264-53421283	MS.gene05623:CDS	50.0%
!!	TTGCAGGTTTGGTGATACCC+TGG	+	chr3.2:53421316-53421335	None:intergenic	50.0%
	AGGCAGGGAAGAACCCTCTA+TGG	-	chr3.2:53421509-53421528	MS.gene05623:CDS	55.0%
	GAGGAAGACTGTGACAGCCA+TGG	-	chr3.2:53421466-53421485	MS.gene05623:CDS	55.0%
	GGAAAGGGAGGTAAGGGACT+TGG	-	chr3.2:53421245-53421264	MS.gene05623:CDS	55.0%
	GGGAGGTAAGGGACTTGGAA+AGG	-	chr3.2:53421250-53421269	MS.gene05623:CDS	55.0%
	GGTAAGGGACTTGGAAAGGG+AGG	-	chr3.2:53421254-53421273	MS.gene05623:CDS	55.0%
	GTCCCTTACCTCCCTTTCCA+CGG	+	chr3.2:53421244-53421263	None:intergenic	55.0%
!	CGTCGATTGGCAAGAAGAGG+TGG	-	chr3.2:53421338-53421357	MS.gene05623:CDS	55.0%
	AGGCCGTGGAAAGGGAGGTA+AGG	-	chr3.2:53421238-53421257	MS.gene05623:CDS	60.0%
	ATGTCAGGCCGTGGAAAGGG+AGG	-	chr3.2:53421233-53421252	MS.gene05623:CDS	60.0%
	GGCCGTGGAAAGGGAGGTAA+GGG	-	chr3.2:53421239-53421258	MS.gene05623:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.2	gene	53421233	53421544	53421233	ID=MS.gene05623
chr3.2	mRNA	53421233	53421544	53421233	ID=MS.gene05623.t1;Parent=MS.gene05623
chr3.2	exon	53421233	53421544	53421233	ID=MS.gene05623.t1.exon1;Parent=MS.gene05623.t1
chr3.2	CDS	53421233	53421544	53421233	ID=cds.MS.gene05623.t1;Parent=MS.gene05623.t1

Gene Sequence	>MS.gene05623 ATGTCAGGCCGTGGAAAGGGAGGTAAGGGACTTGGAAAGGGAGGTGCAAAGAGGCATAGAAAGGTTCTTCGTGATAACATCCAGGGTATCACCAAACCTGCAATTCGTCGATTGGCAAGAAGAGGTGGTGTCAAGAGAATCAGTGGATTGATATATGAAGAAACCAGAGGTGTCTTGAAGATCTTCTTGGAAAATGTCATTCGTGATGCAGTTACATACACTGAGCATGCTAGGAGGAAGACTGTGACAGCCATGGATGTTGTCTATGCTTTGAAGAGGCAGGGAAGAACCCTCTATGGATTTGGAGGTTGA
Protein sequence	>MS.gene05623.t1 MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGG