Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene31359.t1 | KEH39288.1 | 69.5 | 239 | 65 | 2 | 2 | 237 | 1 | 234 | 2.00E-61 | 246.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene31359.t1 | A0A072VCW3 | 69.5 | 239 | 65 | 2 | 2 | 237 | 1 | 234 | 1.4e-61 | 246.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050020 | MS.gene31359 | 0.81397 | 1.95E-51 | -1.69E-46 |
| MS.gene050563 | MS.gene31359 | 0.801918 | 7.18E-49 | -1.69E-46 |
| MS.gene050780 | MS.gene31359 | 0.807865 | 4.10E-50 | -1.69E-46 |
| MS.gene050807 | MS.gene31359 | 0.813016 | 3.17E-51 | -1.69E-46 |
| MS.gene051626 | MS.gene31359 | 0.80858 | 2.89E-50 | -1.69E-46 |
| MS.gene051789 | MS.gene31359 | 0.845452 | 4.03E-59 | -1.69E-46 |
| MS.gene052698 | MS.gene31359 | 0.833006 | 6.83E-56 | -1.69E-46 |
| MS.gene053074 | MS.gene31359 | 0.841983 | 3.41E-58 | -1.69E-46 |
| MS.gene053383 | MS.gene31359 | 0.825686 | 4.09E-54 | -1.69E-46 |
| MS.gene053563 | MS.gene31359 | 0.824593 | 7.42E-54 | -1.69E-46 |
| MS.gene05456 | MS.gene31359 | 0.834251 | 3.34E-56 | -1.69E-46 |
| MS.gene054596 | MS.gene31359 | 0.816458 | 5.47E-52 | -1.69E-46 |
| MS.gene055358 | MS.gene31359 | 0.802237 | 6.17E-49 | -1.69E-46 |
| MS.gene055359 | MS.gene31359 | 0.859767 | 3.29E-63 | -1.69E-46 |
| MS.gene057395 | MS.gene31359 | 0.804816 | 1.80E-49 | -1.69E-46 |
| MS.gene058168 | MS.gene31359 | 0.81724 | 3.65E-52 | -1.69E-46 |
| MS.gene059006 | MS.gene31359 | 0.86391 | 1.78E-64 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene31359.t1 | MTR_2g091315 | 72.081 | 591 | 54 | 7 | 2 | 500 | 1 | 572 | 0.0 | 729 |
| MS.gene31359.t1 | MTR_2g093010 | 62.808 | 527 | 135 | 10 | 1 | 487 | 1 | 506 | 9.66e-177 | 519 |
| MS.gene31359.t1 | MTR_2g093010 | 39.462 | 223 | 131 | 2 | 274 | 496 | 447 | 665 | 1.50e-28 | 120 |
| MS.gene31359.t1 | MTR_2g093010 | 41.778 | 225 | 105 | 2 | 289 | 487 | 406 | 630 | 1.67e-26 | 114 |
| MS.gene31359.t1 | MTR_2g093010 | 62.738 | 526 | 135 | 10 | 2 | 487 | 1 | 505 | 1.00e-175 | 516 |
| MS.gene31359.t1 | MTR_2g093010 | 39.462 | 223 | 131 | 2 | 274 | 496 | 446 | 664 | 1.46e-28 | 120 |
| MS.gene31359.t1 | MTR_2g093010 | 41.778 | 225 | 105 | 2 | 289 | 487 | 405 | 629 | 1.53e-26 | 114 |
| MS.gene31359.t1 | MTR_2g091080 | 61.783 | 471 | 145 | 6 | 34 | 487 | 13 | 465 | 6.69e-166 | 488 |
| MS.gene31359.t1 | MTR_2g091080 | 45.263 | 190 | 104 | 0 | 288 | 477 | 406 | 595 | 4.75e-28 | 118 |
| MS.gene31359.t1 | MTR_2g091080 | 34.866 | 261 | 136 | 9 | 192 | 432 | 353 | 599 | 9.95e-15 | 77.4 |
| MS.gene31359.t1 | MTR_2g091110 | 60.040 | 498 | 149 | 6 | 32 | 487 | 11 | 500 | 1.89e-162 | 494 |
| MS.gene31359.t1 | MTR_2g091110 | 45.973 | 298 | 141 | 5 | 191 | 487 | 383 | 661 | 1.12e-47 | 177 |
| MS.gene31359.t1 | MTR_2g091110 | 38.579 | 394 | 210 | 9 | 102 | 487 | 558 | 927 | 1.44e-40 | 157 |
| MS.gene31359.t1 | MTR_2g091110 | 41.502 | 253 | 128 | 5 | 246 | 487 | 516 | 759 | 1.93e-31 | 129 |
| MS.gene31359.t1 | MTR_2g091110 | 34.584 | 373 | 186 | 9 | 172 | 493 | 674 | 1039 | 6.94e-27 | 115 |
| MS.gene31359.t1 | MTR_2g091245 | 54.976 | 422 | 139 | 7 | 32 | 432 | 14 | 405 | 6.69e-114 | 351 |
| MS.gene31359.t1 | MTR_2g091325 | 42.884 | 534 | 219 | 12 | 1 | 487 | 1 | 495 | 1.97e-98 | 308 |
| MS.gene31359.t1 | MTR_2g091260 | 41.803 | 610 | 152 | 16 | 2 | 481 | 1 | 537 | 8.51e-91 | 296 |
| MS.gene31359.t1 | MTR_2g091260 | 39.437 | 142 | 86 | 0 | 291 | 432 | 473 | 614 | 3.69e-12 | 69.3 |
| MS.gene31359.t1 | MTR_2g091010 | 43.275 | 513 | 225 | 9 | 25 | 489 | 42 | 536 | 2.52e-89 | 289 |
| MS.gene31359.t1 | MTR_2g091010 | 28.895 | 353 | 196 | 9 | 95 | 433 | 281 | 592 | 1.66e-11 | 67.4 |
| MS.gene31359.t1 | MTR_2g091290 | 42.308 | 468 | 223 | 7 | 34 | 479 | 26 | 468 | 1.21e-81 | 270 |
| MS.gene31359.t1 | MTR_2g091015 | 39.897 | 584 | 185 | 15 | 6 | 487 | 6 | 525 | 3.16e-81 | 270 |
| MS.gene31359.t1 | MTR_2g091015 | 40.433 | 277 | 122 | 6 | 244 | 486 | 369 | 636 | 5.56e-31 | 127 |
| MS.gene31359.t1 | MTR_2g091015 | 41.199 | 267 | 145 | 6 | 225 | 490 | 423 | 678 | 3.23e-29 | 122 |
| MS.gene31359.t1 | MTR_2g091015 | 36.620 | 213 | 125 | 1 | 288 | 490 | 522 | 734 | 1.20e-21 | 99.4 |
| MS.gene31359.t1 | MTR_2g091370 | 42.593 | 540 | 236 | 11 | 2 | 489 | 1 | 518 | 2.18e-80 | 267 |
| MS.gene31359.t1 | MTR_6g045183 | 43.048 | 525 | 216 | 10 | 1 | 489 | 1 | 478 | 1.49e-79 | 263 |
| MS.gene31359.t1 | MTR_2g104190 | 52.632 | 342 | 148 | 4 | 67 | 408 | 122 | 449 | 5.92e-79 | 259 |
| MS.gene31359.t1 | MTR_2g104190 | 34.667 | 150 | 93 | 2 | 338 | 487 | 272 | 416 | 4.50e-11 | 65.9 |
| MS.gene31359.t1 | MTR_2g091280 | 38.971 | 544 | 250 | 9 | 6 | 492 | 6 | 524 | 4.32e-77 | 256 |
| MS.gene31359.t1 | MTR_2g091280 | 33.489 | 427 | 248 | 10 | 79 | 475 | 297 | 717 | 8.01e-27 | 115 |
| MS.gene31359.t1 | MTR_2g091280 | 34.190 | 389 | 223 | 9 | 128 | 489 | 307 | 689 | 5.07e-25 | 109 |
| MS.gene31359.t1 | MTR_2g091250 | 41.176 | 493 | 178 | 11 | 6 | 487 | 17 | 408 | 4.29e-73 | 252 |
| MS.gene31359.t1 | MTR_2g091250 | 49.275 | 207 | 91 | 1 | 288 | 480 | 615 | 821 | 1.11e-35 | 142 |
| MS.gene31359.t1 | MTR_2g091250 | 45.631 | 206 | 110 | 1 | 288 | 493 | 489 | 692 | 3.65e-34 | 137 |
| MS.gene31359.t1 | MTR_2g091250 | 34.186 | 430 | 207 | 10 | 128 | 485 | 520 | 945 | 1.19e-31 | 130 |
| MS.gene31359.t1 | MTR_2g091250 | 45.000 | 200 | 110 | 0 | 288 | 487 | 531 | 730 | 2.37e-31 | 129 |
| MS.gene31359.t1 | MTR_2g091250 | 33.251 | 403 | 172 | 12 | 170 | 477 | 628 | 1028 | 6.54e-25 | 109 |
| MS.gene31359.t1 | MTR_2g091250 | 39.375 | 160 | 82 | 3 | 280 | 432 | 881 | 1032 | 1.11e-14 | 77.8 |
| MS.gene31359.t1 | MTR_2g091005 | 38.641 | 515 | 250 | 12 | 2 | 487 | 1 | 478 | 1.46e-68 | 231 |
| MS.gene31359.t1 | MTR_2g091335 | 36.917 | 493 | 249 | 11 | 2 | 472 | 1 | 453 | 1.43e-67 | 228 |
| MS.gene31359.t1 | MTR_2g091105 | 42.857 | 434 | 176 | 11 | 32 | 427 | 15 | 414 | 1.40e-66 | 227 |
| MS.gene31359.t1 | MTR_2g091105 | 34.375 | 288 | 150 | 6 | 216 | 489 | 130 | 392 | 5.87e-17 | 84.3 |
| MS.gene31359.t1 | MTR_7g056313 | 45.210 | 334 | 169 | 5 | 67 | 400 | 50 | 369 | 8.22e-64 | 212 |
| MS.gene31359.t1 | MTR_3g110385 | 39.224 | 464 | 186 | 10 | 67 | 487 | 34 | 444 | 5.06e-60 | 211 |
| MS.gene31359.t1 | MTR_3g110385 | 46.119 | 219 | 99 | 2 | 288 | 487 | 329 | 547 | 4.56e-32 | 130 |
| MS.gene31359.t1 | MTR_3g110385 | 42.754 | 138 | 74 | 1 | 288 | 420 | 427 | 564 | 2.66e-14 | 76.3 |
| MS.gene31359.t1 | MTR_2g091330 | 38.704 | 540 | 231 | 12 | 5 | 481 | 30 | 532 | 1.19e-58 | 204 |
| MS.gene31359.t1 | MTR_2g091330 | 38.251 | 183 | 92 | 2 | 288 | 470 | 430 | 591 | 7.76e-19 | 90.1 |
| MS.gene31359.t1 | MTR_5g078500 | 39.523 | 377 | 139 | 9 | 2 | 367 | 1 | 299 | 3.98e-57 | 192 |
| MS.gene31359.t1 | MTR_5g078500 | 34.574 | 188 | 106 | 5 | 293 | 470 | 124 | 304 | 2.63e-11 | 65.1 |
| MS.gene31359.t1 | MTR_2g091030 | 38.136 | 472 | 204 | 10 | 2 | 440 | 1 | 417 | 2.43e-56 | 197 |
| MS.gene31359.t1 | MTR_2g091030 | 35.000 | 260 | 137 | 4 | 220 | 479 | 148 | 375 | 2.07e-20 | 94.7 |
| MS.gene31359.t1 | MTR_2g010420 | 37.381 | 420 | 232 | 7 | 65 | 480 | 50 | 442 | 1.76e-54 | 195 |
| MS.gene31359.t1 | MTR_2g010420 | 40.104 | 192 | 101 | 1 | 289 | 480 | 335 | 512 | 6.49e-24 | 105 |
| MS.gene31359.t1 | MTR_2g010420 | 37.037 | 216 | 112 | 4 | 277 | 491 | 378 | 570 | 2.39e-19 | 92.0 |
| MS.gene31359.t1 | MTR_2g010420 | 32.143 | 252 | 163 | 3 | 237 | 488 | 137 | 380 | 1.48e-12 | 70.5 |
| MS.gene31359.t1 | MTR_2g010420 | 29.819 | 332 | 201 | 8 | 125 | 434 | 250 | 571 | 7.36e-11 | 65.1 |
| MS.gene31359.t1 | MTR_2g010420 | 37.381 | 420 | 232 | 7 | 65 | 480 | 50 | 442 | 4.57e-54 | 195 |
| MS.gene31359.t1 | MTR_2g010420 | 40.104 | 192 | 101 | 1 | 289 | 480 | 335 | 512 | 4.68e-24 | 106 |
| MS.gene31359.t1 | MTR_2g010420 | 37.037 | 216 | 112 | 4 | 277 | 491 | 378 | 570 | 1.87e-19 | 92.4 |
| MS.gene31359.t1 | MTR_2g010420 | 32.143 | 252 | 163 | 3 | 237 | 488 | 137 | 380 | 1.32e-12 | 70.9 |
| MS.gene31359.t1 | MTR_2g010420 | 35.374 | 147 | 88 | 1 | 288 | 434 | 432 | 571 | 7.39e-11 | 65.1 |
| MS.gene31359.t1 | MTR_5g038810 | 40.260 | 385 | 154 | 9 | 48 | 427 | 11 | 324 | 1.33e-53 | 191 |
| MS.gene31359.t1 | MTR_5g038810 | 37.900 | 219 | 111 | 3 | 237 | 441 | 117 | 324 | 6.63e-25 | 108 |
| MS.gene31359.t1 | MTR_5g038810 | 34.630 | 257 | 146 | 4 | 231 | 487 | 80 | 314 | 1.30e-19 | 92.8 |
| MS.gene31359.t1 | MTR_2g091040 | 36.495 | 485 | 187 | 14 | 1 | 456 | 1 | 393 | 2.27e-52 | 186 |
| MS.gene31359.t1 | MTR_2g091285 | 35.604 | 455 | 210 | 9 | 6 | 426 | 6 | 411 | 1.55e-51 | 185 |
| MS.gene31359.t1 | MTR_2g091090 | 36.701 | 485 | 186 | 14 | 1 | 456 | 1 | 393 | 2.28e-51 | 185 |
| MS.gene31359.t1 | MTR_2g090640 | 45.277 | 307 | 107 | 4 | 240 | 485 | 101 | 407 | 1.20e-46 | 170 |
| MS.gene31359.t1 | MTR_2g090640 | 33.636 | 330 | 179 | 6 | 167 | 461 | 101 | 425 | 5.93e-25 | 108 |
| MS.gene31359.t1 | MTR_2g090640 | 44.805 | 154 | 68 | 2 | 345 | 481 | 98 | 251 | 2.47e-18 | 88.2 |
| MS.gene31359.t1 | MTR_2g090640 | 33.607 | 366 | 184 | 11 | 108 | 461 | 115 | 433 | 1.83e-16 | 82.4 |
| MS.gene31359.t1 | MTR_2g091240 | 43.878 | 294 | 118 | 4 | 194 | 475 | 57 | 315 | 9.60e-45 | 166 |
| MS.gene31359.t1 | MTR_2g091240 | 28.266 | 421 | 207 | 9 | 12 | 432 | 3 | 328 | 1.45e-18 | 89.4 |
| MS.gene31359.t1 | MTR_5g078510 | 44.749 | 219 | 98 | 3 | 290 | 487 | 1 | 217 | 3.30e-26 | 112 |
| MS.gene31359.t1 | MTR_5g078510 | 38.559 | 236 | 133 | 2 | 246 | 474 | 32 | 262 | 3.65e-19 | 90.9 |
| MS.gene31359.t1 | MTR_5g078510 | 40.157 | 127 | 62 | 1 | 376 | 488 | 3 | 129 | 2.91e-12 | 69.3 |
| MS.gene31359.t1 | MTR_5g078510 | 31.128 | 257 | 166 | 2 | 225 | 477 | 30 | 279 | 4.28e-12 | 68.9 |
| MS.gene31359.t1 | MTR_2g091025 | 41.026 | 195 | 112 | 1 | 297 | 488 | 136 | 330 | 3.97e-26 | 112 |
| MS.gene31359.t1 | MTR_2g091025 | 34.320 | 169 | 94 | 2 | 288 | 439 | 169 | 337 | 4.77e-14 | 75.1 |
| MS.gene31359.t1 | MTR_4g084920 | 37.173 | 191 | 106 | 4 | 123 | 311 | 12 | 190 | 1.36e-17 | 84.0 |
| MS.gene31359.t1 | MTR_2g091360 | 42.446 | 139 | 70 | 1 | 295 | 433 | 41 | 169 | 3.11e-17 | 83.2 |
| MS.gene31359.t1 | MTR_2g091360 | 39.597 | 149 | 81 | 2 | 333 | 475 | 24 | 169 | 5.36e-14 | 73.6 |
| MS.gene31359.t1 | MTR_2g103640 | 38.065 | 155 | 83 | 2 | 327 | 481 | 85 | 226 | 9.22e-11 | 63.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 84 sgRNAs with CRISPR-Local
Find 111 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACACCTTTGATTTCTTAAC+AGG | 0.253750 | 2.4:+8983211 | None:intergenic |
| GATGCAGTGAACAGATTAAT+TGG | 0.298405 | 2.4:-8982668 | MS.gene31359:CDS |
| CAAGGAGCTGGAATTTAAAA+AGG | 0.308710 | 2.4:-8982228 | MS.gene31359:CDS |
| TGCATCAAGCTCGTTCTCTT+TGG | 0.311415 | 2.4:+8982685 | None:intergenic |
| AACAGAAACATTATGAAAAC+TGG | 0.318986 | 2.4:-8982169 | MS.gene31359:CDS |
| TAAATTCCAGCTCCTTGATT+TGG | 0.319233 | 2.4:+8982234 | None:intergenic |
| AATTATTCGAGGATTGTAAA+AGG | 0.329876 | 2.4:-8983093 | MS.gene31359:CDS |
| TGAATTTACTTTGGAATGTA+AGG | 0.352735 | 2.4:-8982708 | MS.gene31359:CDS |
| CTAAAATACCATCTTTCAAT+TGG | 0.362943 | 2.4:-8981977 | MS.gene31359:CDS |
| ATCATTGTTGTGATCATGAT+AGG | 0.364681 | 2.4:+8983186 | None:intergenic |
| TATTTGATAGAAAACTTGAT+TGG | 0.371620 | 2.4:-8982887 | MS.gene31359:CDS |
| GTCCTCAATATCTGTCTTGA+TGG | 0.384277 | 2.4:+8983039 | None:intergenic |
| TTCAATTGGTGGATCAGAAA+AGG | 0.384755 | 2.4:-8981963 | MS.gene31359:CDS |
| TTGATTTGAGCTCCTCCACT+TGG | 0.428961 | 2.4:+8982024 | None:intergenic |
| TTTCTTAACAGGAATATGTA+AGG | 0.437226 | 2.4:+8983222 | None:intergenic |
| AAATACCATCTTTCAATTGG+TGG | 0.441983 | 2.4:-8981974 | MS.gene31359:CDS |
| TATCAAGGAGTTTGTGGTAA+AGG | 0.444893 | 2.4:-8982921 | MS.gene31359:CDS |
| CTGATCCACCAATTGAAAGA+TGG | 0.446568 | 2.4:+8981969 | None:intergenic |
| CTCGAAACCCGGGAAAATCT+TGG | 0.447593 | 2.4:+8983322 | None:intergenic |
| TCAAGAGAGAAGCAGCTTGA+AGG | 0.454073 | 2.4:-8982425 | MS.gene31359:CDS |
| TCAATTGGTGGATCAGAAAA+GGG | 0.462098 | 2.4:-8981962 | MS.gene31359:CDS |
| AAAGATGCAACTTGAAGGTC+GGG | 0.465394 | 2.4:-8982294 | MS.gene31359:CDS |
| GCTAGTTGAACGACGGGCAA+TGG | 0.479453 | 2.4:-8982330 | MS.gene31359:CDS |
| TTGTGCTCAAATGAAAGAAT+TGG | 0.494143 | 2.4:-8982576 | MS.gene31359:CDS |
| AGTCATTGATAATATTGATA+AGG | 0.496791 | 2.4:-8982780 | MS.gene31359:CDS |
| TGAAAGTCAAATGAAGGAGT+TGG | 0.496818 | 2.4:-8982198 | MS.gene31359:CDS |
| TGAAGGCAAAGTGAAGGATC+TGG | 0.496975 | 2.4:-8982072 | MS.gene31359:CDS |
| GAAAGTTGAAGGCCAAATCA+AGG | 0.499096 | 2.4:-8982246 | MS.gene31359:CDS |
| TCAACGAAGAAGCGACTTGA+AGG | 0.501483 | 2.4:-8982551 | MS.gene31359:CDS |
| GCAACATGAAGGCCGGGTGA+TGG | 0.501679 | 2.4:-8982120 | MS.gene31359:CDS |
| GAAGGAGTTTAAATCAAAAG+AGG | 0.503664 | 2.4:-8982270 | MS.gene31359:CDS |
| GAAGGAGTTTGAATCAAAGA+TGG | 0.508553 | 2.4:-8982396 | MS.gene31359:CDS |
| CATCACCAACATAAAATTGA+GGG | 0.510264 | 2.4:+8983407 | None:intergenic |
| ATGAGAAGCAACATGAAGGC+CGG | 0.512889 | 2.4:-8982127 | MS.gene31359:CDS |
| TGAAGGCCAAATCAAGGAGC+TGG | 0.513684 | 2.4:-8982240 | MS.gene31359:CDS |
| TGATTGTATCAAGGAGTTTG+TGG | 0.516424 | 2.4:-8982927 | MS.gene31359:CDS |
| TCATCACCAACATAAAATTG+AGG | 0.518201 | 2.4:+8983406 | None:intergenic |
| TAGTAGTGCTGTTAAGAAGA+GGG | 0.518338 | 2.4:-8983275 | MS.gene31359:CDS |
| GAAGCAATGTGAAGGACGGC+TGG | 0.519439 | 2.4:-8982501 | MS.gene31359:CDS |
| GTTGCTTCTCATTTAAGTCA+AGG | 0.519662 | 2.4:+8982138 | None:intergenic |
| GGACCGTGAAAGGAAGGAAA+AGG | 0.523989 | 2.4:-8982759 | MS.gene31359:CDS |
| TCAAAAGAGGAGAAAGTTGA+AGG | 0.524009 | 2.4:-8982257 | MS.gene31359:CDS |
| GCTGGAGGACCTTGTGTCAA+AGG | 0.524528 | 2.4:-8982483 | MS.gene31359:CDS |
| GTCCATCAAGACAGATATTG+AGG | 0.531510 | 2.4:-8983041 | MS.gene31359:CDS |
| TCAAAAGAGAGGCAAAATCA+AGG | 0.534054 | 2.4:-8982005 | MS.gene31359:CDS |
| GCTAAAGGTAACCTCCGAAA+AGG | 0.534105 | 2.4:-8982606 | MS.gene31359:CDS |
| GCACTTTGAAAGTCAAATGA+AGG | 0.537688 | 2.4:-8982204 | MS.gene31359:CDS |
| GAAGAAGCGACTTGAAGGTC+AGG | 0.538700 | 2.4:-8982546 | MS.gene31359:CDS |
| TGAGAAGCAACATGAAGGCC+GGG | 0.540036 | 2.4:-8982126 | MS.gene31359:CDS |
| AGAGAAGCTAGTTGAACGAC+GGG | 0.541148 | 2.4:-8982336 | MS.gene31359:CDS |
| TCAAAGATGGAGGAACTTGA+AGG | 0.541445 | 2.4:-8982383 | MS.gene31359:CDS |
| TTGCTTCATGCTCCATCACC+CGG | 0.542096 | 2.4:+8982108 | None:intergenic |
| GCAGCTTGAAGGATATATGA+AGG | 0.544518 | 2.4:-8982414 | MS.gene31359:CDS |
| AAGGAGTTTGTGGTAAAGGA+AGG | 0.548806 | 2.4:-8982917 | MS.gene31359:CDS |
| GTAGTAGTGCTGTTAAGAAG+AGG | 0.550710 | 2.4:-8983276 | MS.gene31359:CDS |
| AAAAGATGCAACTTGAAGGT+CGG | 0.554162 | 2.4:-8982295 | MS.gene31359:CDS |
| GATAGAACTTAATCAAGTCA+AGG | 0.560272 | 2.4:-8982834 | MS.gene31359:CDS |
| TGTAAGGTCTTTCTTGAGCG+TGG | 0.560417 | 2.4:+8983126 | None:intergenic |
| TGATAAGGACCGTGAAAGGA+AGG | 0.560715 | 2.4:-8982765 | MS.gene31359:CDS |
| AGAATTTGAAGGCAAAGTGA+AGG | 0.571180 | 2.4:-8982078 | MS.gene31359:CDS |
| GCAACTTGAAGGTCGGGAGA+AGG | 0.574045 | 2.4:-8982288 | MS.gene31359:CDS |
| GGAAGGGAACAAGTTGCTAA+AGG | 0.576054 | 2.4:-8982621 | MS.gene31359:CDS |
| GGAACTTGAAGGTCGTTCAA+AGG | 0.582951 | 2.4:-8982372 | MS.gene31359:CDS |
| ATAGAACTTAATCAAGTCAA+GGG | 0.583347 | 2.4:-8982833 | MS.gene31359:CDS |
| AGAGCGCAAGCTAGAGCTCA+AGG | 0.583452 | 2.4:-8982864 | MS.gene31359:CDS |
| AAGGAAGGAAAAGGAACTCA+AGG | 0.588717 | 2.4:-8982750 | MS.gene31359:CDS |
| TCATTGTTGTGATCATGATA+GGG | 0.596216 | 2.4:+8983187 | None:intergenic |
| GCAAAGGTGAGAGAATTTGA+AGG | 0.600132 | 2.4:-8982089 | MS.gene31359:CDS |
| TGAAGTTCGTGAGAAAGTGC+AGG | 0.603159 | 2.4:-8982960 | MS.gene31359:CDS |
| GAAACATTATGAAAACTGGA+TGG | 0.606274 | 2.4:-8982165 | MS.gene31359:CDS |
| CGGGAAAATCTTGGTAACAA+AGG | 0.608995 | 2.4:+8983331 | None:intergenic |
| GGAGTTTGAATCAAAGATGG+AGG | 0.617704 | 2.4:-8982393 | MS.gene31359:CDS |
| TTAAATGAGAAGCAACATGA+AGG | 0.618712 | 2.4:-8982131 | MS.gene31359:CDS |
| ATATTGATAAGGACCGTGAA+AGG | 0.619247 | 2.4:-8982769 | MS.gene31359:CDS |
| AAGAGAAGCAATGTGAAGGA+CGG | 0.619590 | 2.4:-8982505 | MS.gene31359:CDS |
| TCAAAAGAGAAGCAATGTGA+AGG | 0.628240 | 2.4:-8982509 | MS.gene31359:CDS |
| GGTGATGGAGCATGAAGCAA+AGG | 0.629813 | 2.4:-8982105 | MS.gene31359:CDS |
| AGGAGTTTGTGGTAAAGGAA+GGG | 0.632276 | 2.4:-8982916 | MS.gene31359:CDS |
| GCAATGTGAAGGACGGCTGG+AGG | 0.637067 | 2.4:-8982498 | MS.gene31359:CDS |
| TATTCCTGTTAAGAAATCAA+AGG | 0.641017 | 2.4:-8983215 | MS.gene31359:CDS |
| AATGCTTCTCCTTTGACACA+AGG | 0.644906 | 2.4:+8982474 | None:intergenic |
| AAGAGAAGCTAGTTGAACGA+CGG | 0.649590 | 2.4:-8982337 | MS.gene31359:CDS |
| AGGAGCTCAAATCAAAAGAG+AGG | 0.654158 | 2.4:-8982016 | MS.gene31359:CDS |
| ACATTATGAAAACTGGATGG+AGG | 0.687349 | 2.4:-8982162 | MS.gene31359:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAATTGATGAATTTACTT+TGG | - | chr2.4:8982669-8982688 | MS.gene31359:CDS | 15.0% |
| !!! | AAGTAAATTCATCAATTTTT+TGG | + | chr2.4:8982669-8982688 | None:intergenic | 15.0% |
| !!! | AGTAAATTCATCAATTTTTT+GGG | + | chr2.4:8982668-8982687 | None:intergenic | 15.0% |
| !!! | ATAATTTTTCTAACAATGTA+AGG | + | chr2.4:8982279-8982298 | None:intergenic | 15.0% |
| !! | ATTGTTAGAAAAATTATTCG+AGG | - | chr2.4:8982282-8982301 | MS.gene31359:CDS | 20.0% |
| !! | TATTTGATAGAAAACTTGAT+TGG | - | chr2.4:8982499-8982518 | MS.gene31359:CDS | 20.0% |
| !!! | AAAAATTGATGATTGTATCA+AGG | - | chr2.4:8982450-8982469 | MS.gene31359:CDS | 20.0% |
| !!! | AGTCATTGATAATATTGATA+AGG | - | chr2.4:8982606-8982625 | MS.gene31359:CDS | 20.0% |
| ! | AACAGAAACATTATGAAAAC+TGG | - | chr2.4:8983217-8983236 | MS.gene31359:CDS | 25.0% |
| ! | AATTATTCGAGGATTGTAAA+AGG | - | chr2.4:8982293-8982312 | MS.gene31359:CDS | 25.0% |
| ! | ATAGAACTTAATCAAGTCAA+GGG | - | chr2.4:8982553-8982572 | MS.gene31359:CDS | 25.0% |
| ! | CTAAAATACCATCTTTCAAT+TGG | - | chr2.4:8983409-8983428 | MS.gene31359:CDS | 25.0% |
| ! | TATTCCTGTTAAGAAATCAA+AGG | - | chr2.4:8982171-8982190 | MS.gene31359:CDS | 25.0% |
| ! | TTTCTTAACAGGAATATGTA+AGG | + | chr2.4:8982167-8982186 | None:intergenic | 25.0% |
| !!! | TGAATTTACTTTGGAATGTA+AGG | - | chr2.4:8982678-8982697 | MS.gene31359:CDS | 25.0% |
| AAATACCATCTTTCAATTGG+TGG | - | chr2.4:8983412-8983431 | MS.gene31359:CDS | 30.0% | |
| ACAAGCAGAAAAATTAGAGT+CGG | - | chr2.4:8982744-8982763 | MS.gene31359:CDS | 30.0% | |
| ATCATTGTTGTGATCATGAT+AGG | + | chr2.4:8982203-8982222 | None:intergenic | 30.0% | |
| CATCACCAACATAAAATTGA+GGG | + | chr2.4:8981982-8982001 | None:intergenic | 30.0% | |
| GAAACATTATGAAAACTGGA+TGG | - | chr2.4:8983221-8983240 | MS.gene31359:CDS | 30.0% | |
| GAAGGAGTTTAAATCAAAAG+AGG | - | chr2.4:8983116-8983135 | MS.gene31359:CDS | 30.0% | |
| GATAGAACTTAATCAAGTCA+AGG | - | chr2.4:8982552-8982571 | MS.gene31359:CDS | 30.0% | |
| TCAAAAAAGATGCAACTTGA+AGG | - | chr2.4:8983087-8983106 | MS.gene31359:CDS | 30.0% | |
| TCATCACCAACATAAAATTG+AGG | + | chr2.4:8981983-8982002 | None:intergenic | 30.0% | |
| TCATTGTTGTGATCATGATA+GGG | + | chr2.4:8982202-8982221 | None:intergenic | 30.0% | |
| TTAAATGAGAAGCAACATGA+AGG | - | chr2.4:8983255-8983274 | MS.gene31359:CDS | 30.0% | |
| TTGTGCTCAAATGAAAGAAT+TGG | - | chr2.4:8982810-8982829 | MS.gene31359:CDS | 30.0% | |
| ! | AACACCTTTGATTTCTTAAC+AGG | + | chr2.4:8982178-8982197 | None:intergenic | 30.0% |
| ! | CATAACCCTCAATTTTATGT+TGG | - | chr2.4:8981974-8981993 | MS.gene31359:CDS | 30.0% |
| !!! | AATTTTATGTTGGTGATGAG+AGG | - | chr2.4:8981984-8982003 | MS.gene31359:CDS | 30.0% |
| !!! | ATTTTATGTTGGTGATGAGA+GGG | - | chr2.4:8981985-8982004 | MS.gene31359:CDS | 30.0% |
| AAAAGATGCAACTTGAAGGT+CGG | - | chr2.4:8983091-8983110 | MS.gene31359:CDS | 35.0% | |
| ACATTATGAAAACTGGATGG+AGG | - | chr2.4:8983224-8983243 | MS.gene31359:CDS | 35.0% | |
| AGAATTTGAAGGCAAAGTGA+AGG | - | chr2.4:8983308-8983327 | MS.gene31359:CDS | 35.0% | |
| ATATTGATAAGGACCGTGAA+AGG | - | chr2.4:8982617-8982636 | MS.gene31359:CDS | 35.0% | |
| CAAGGAGCTGGAATTTAAAA+AGG | - | chr2.4:8983158-8983177 | MS.gene31359:CDS | 35.0% | |
| CAGAAAAATTAGAGTCGGAA+GGG | - | chr2.4:8982749-8982768 | MS.gene31359:CDS | 35.0% | |
| GAAGGAGTTTGAATCAAAGA+TGG | - | chr2.4:8982990-8983009 | MS.gene31359:CDS | 35.0% | |
| GATGCAGTGAACAGATTAAT+TGG | - | chr2.4:8982718-8982737 | MS.gene31359:CDS | 35.0% | |
| GTTGCTTCTCATTTAAGTCA+AGG | + | chr2.4:8983251-8983270 | None:intergenic | 35.0% | |
| TAAATTCCAGCTCCTTGATT+TGG | + | chr2.4:8983155-8983174 | None:intergenic | 35.0% | |
| TATCAAGGAGTTTGTGGTAA+AGG | - | chr2.4:8982465-8982484 | MS.gene31359:CDS | 35.0% | |
| TCAAAAGAGAAGCAATGTGA+AGG | - | chr2.4:8982877-8982896 | MS.gene31359:CDS | 35.0% | |
| TCAAAAGAGAGGCAAAATCA+AGG | - | chr2.4:8983381-8983400 | MS.gene31359:CDS | 35.0% | |
| TCAAAAGAGGAGAAAGTTGA+AGG | - | chr2.4:8983129-8983148 | MS.gene31359:CDS | 35.0% | |
| TGAAAGTCAAATGAAGGAGT+TGG | - | chr2.4:8983188-8983207 | MS.gene31359:CDS | 35.0% | |
| TGATTGTATCAAGGAGTTTG+TGG | - | chr2.4:8982459-8982478 | MS.gene31359:CDS | 35.0% | |
| ! | AGCACAATCATTCTCCTTTT+CGG | + | chr2.4:8982797-8982816 | None:intergenic | 35.0% |
| ! | ATTTTTCCAAACTCGAAACC+CGG | + | chr2.4:8982078-8982097 | None:intergenic | 35.0% |
| ! | CTTTGTTACCAAGATTTTCC+CGG | - | chr2.4:8982056-8982075 | MS.gene31359:CDS | 35.0% |
| ! | GCACTTTGAAAGTCAAATGA+AGG | - | chr2.4:8983182-8983201 | MS.gene31359:CDS | 35.0% |
| ! | TAGTAGTGCTGTTAAGAAGA+GGG | - | chr2.4:8982111-8982130 | MS.gene31359:CDS | 35.0% |
| ! | TCAATTGGTGGATCAGAAAA+GGG | - | chr2.4:8983424-8983443 | MS.gene31359:CDS | 35.0% |
| ! | TTCAATTGGTGGATCAGAAA+AGG | - | chr2.4:8983423-8983442 | MS.gene31359:CDS | 35.0% |
| ! | TTTGTTACCAAGATTTTCCC+GGG | - | chr2.4:8982057-8982076 | MS.gene31359:CDS | 35.0% |
| AAAGATGCAACTTGAAGGTC+GGG | - | chr2.4:8983092-8983111 | MS.gene31359:CDS | 40.0% | |
| AAGAGAAGCAATGTGAAGGA+CGG | - | chr2.4:8982881-8982900 | MS.gene31359:CDS | 40.0% | |
| AAGAGAAGCTAGTTGAACGA+CGG | - | chr2.4:8983049-8983068 | MS.gene31359:CDS | 40.0% | |
| AAGGAAGGAAAAGGAACTCA+AGG | - | chr2.4:8982636-8982655 | MS.gene31359:CDS | 40.0% | |
| AAGGAGTTTGTGGTAAAGGA+AGG | - | chr2.4:8982469-8982488 | MS.gene31359:CDS | 40.0% | |
| AATGCTTCTCCTTTGACACA+AGG | + | chr2.4:8982915-8982934 | None:intergenic | 40.0% | |
| AGGAGCTCAAATCAAAAGAG+AGG | - | chr2.4:8983370-8983389 | MS.gene31359:CDS | 40.0% | |
| AGGAGTTTGTGGTAAAGGAA+GGG | - | chr2.4:8982470-8982489 | MS.gene31359:CDS | 40.0% | |
| CTGATCCACCAATTGAAAGA+TGG | + | chr2.4:8983420-8983439 | None:intergenic | 40.0% | |
| GAAAGTTGAAGGCCAAATCA+AGG | - | chr2.4:8983140-8983159 | MS.gene31359:CDS | 40.0% | |
| GCAAAGGTGAGAGAATTTGA+AGG | - | chr2.4:8983297-8983316 | MS.gene31359:CDS | 40.0% | |
| GCAGAAAAATTAGAGTCGGA+AGG | - | chr2.4:8982748-8982767 | MS.gene31359:CDS | 40.0% | |
| GCAGCTTGAAGGATATATGA+AGG | - | chr2.4:8982972-8982991 | MS.gene31359:CDS | 40.0% | |
| GGAGTTTGAATCAAAGATGG+AGG | - | chr2.4:8982993-8983012 | MS.gene31359:CDS | 40.0% | |
| GTCCATCAAGACAGATATTG+AGG | - | chr2.4:8982345-8982364 | MS.gene31359:CDS | 40.0% | |
| GTCCTCAATATCTGTCTTGA+TGG | + | chr2.4:8982350-8982369 | None:intergenic | 40.0% | |
| TCAAAGATGGAGGAACTTGA+AGG | - | chr2.4:8983003-8983022 | MS.gene31359:CDS | 40.0% | |
| ! | ACAATCATTCTCCTTTTCGG+AGG | + | chr2.4:8982794-8982813 | None:intergenic | 40.0% |
| ! | CGGGAAAATCTTGGTAACAA+AGG | + | chr2.4:8982058-8982077 | None:intergenic | 40.0% |
| ! | GTAGTAGTGCTGTTAAGAAG+AGG | - | chr2.4:8982110-8982129 | MS.gene31359:CDS | 40.0% |
| ! | GTTCCTTTTCCTTCCTTTCA+CGG | + | chr2.4:8982633-8982652 | None:intergenic | 40.0% |
| ! | TTTTTCCAAACTCGAAACCC+GGG | + | chr2.4:8982077-8982096 | None:intergenic | 40.0% |
| !! | GAAGCATTTTGAAAGCCAAG+TGG | - | chr2.4:8983347-8983366 | MS.gene31359:CDS | 40.0% |
| !!! | AGGAGAAGCATTTTGAGGTA+CGG | - | chr2.4:8982923-8982942 | MS.gene31359:CDS | 40.0% |
| !!! | GTCAAAGGAGAAGCATTTTG+AGG | - | chr2.4:8982918-8982937 | MS.gene31359:CDS | 40.0% |
| AGAGAAGCTAGTTGAACGAC+GGG | - | chr2.4:8983050-8983069 | MS.gene31359:CDS | 45.0% | |
| ATGAGAAGCAACATGAAGGC+CGG | - | chr2.4:8983259-8983278 | MS.gene31359:CDS | 45.0% | |
| GCTAAAGGTAACCTCCGAAA+AGG | - | chr2.4:8982780-8982799 | MS.gene31359:CDS | 45.0% | |
| GGAACTTGAAGGTCGTTCAA+AGG | - | chr2.4:8983014-8983033 | MS.gene31359:CDS | 45.0% | |
| GGAAGGGAACAAGTTGCTAA+AGG | - | chr2.4:8982765-8982784 | MS.gene31359:CDS | 45.0% | |
| TCAACGAAGAAGCGACTTGA+AGG | - | chr2.4:8982835-8982854 | MS.gene31359:CDS | 45.0% | |
| TCAAGAGAGAAGCAGCTTGA+AGG | - | chr2.4:8982961-8982980 | MS.gene31359:CDS | 45.0% | |
| TGAAGGCAAAGTGAAGGATC+TGG | - | chr2.4:8983314-8983333 | MS.gene31359:CDS | 45.0% | |
| TGATAAGGACCGTGAAAGGA+AGG | - | chr2.4:8982621-8982640 | MS.gene31359:CDS | 45.0% | |
| TGCATCAAGCTCGTTCTCTT+TGG | + | chr2.4:8982704-8982723 | None:intergenic | 45.0% | |
| TGTAAGGTCTTTCTTGAGCG+TGG | + | chr2.4:8982263-8982282 | None:intergenic | 45.0% | |
| ! | TGAAGTTCGTGAGAAAGTGC+AGG | - | chr2.4:8982426-8982445 | MS.gene31359:CDS | 45.0% |
| ! | TTGATTTGAGCTCCTCCACT+TGG | + | chr2.4:8983365-8983384 | None:intergenic | 45.0% |
| !! | GCATTTTGAAAGCCAAGTGG+AGG | - | chr2.4:8983350-8983369 | MS.gene31359:CDS | 45.0% |
| !!! | ATTTTCCCGGGTTTCGAGTT+TGG | - | chr2.4:8982069-8982088 | MS.gene31359:CDS | 45.0% |
| !!! | GCATTTTGAGGTACGGGTAA+AGG | - | chr2.4:8982930-8982949 | MS.gene31359:CDS | 45.0% |
| !!! | GGAGAAGCATTTTGAGGTAC+GGG | - | chr2.4:8982924-8982943 | MS.gene31359:CDS | 45.0% |
| CTCGAAACCCGGGAAAATCT+TGG | + | chr2.4:8982067-8982086 | None:intergenic | 50.0% | |
| GAAGAAGCGACTTGAAGGTC+AGG | - | chr2.4:8982840-8982859 | MS.gene31359:CDS | 50.0% | |
| GGACCGTGAAAGGAAGGAAA+AGG | - | chr2.4:8982627-8982646 | MS.gene31359:CDS | 50.0% | |
| TGAAGGCCAAATCAAGGAGC+TGG | - | chr2.4:8983146-8983165 | MS.gene31359:CDS | 50.0% | |
| TGAGAAGCAACATGAAGGCC+GGG | - | chr2.4:8983260-8983279 | MS.gene31359:CDS | 50.0% | |
| TTGCTTCATGCTCCATCACC+CGG | + | chr2.4:8983281-8983300 | None:intergenic | 50.0% | |
| !! | GGTGATGGAGCATGAAGCAA+AGG | - | chr2.4:8983281-8983300 | MS.gene31359:CDS | 50.0% |
| AGAGCGCAAGCTAGAGCTCA+AGG | - | chr2.4:8982522-8982541 | MS.gene31359:CDS | 55.0% | |
| GAAGCAATGTGAAGGACGGC+TGG | - | chr2.4:8982885-8982904 | MS.gene31359:CDS | 55.0% | |
| GCAACTTGAAGGTCGGGAGA+AGG | - | chr2.4:8983098-8983117 | MS.gene31359:CDS | 55.0% | |
| GCTAGTTGAACGACGGGCAA+TGG | - | chr2.4:8983056-8983075 | MS.gene31359:CDS | 55.0% | |
| GCTGGAGGACCTTGTGTCAA+AGG | - | chr2.4:8982903-8982922 | MS.gene31359:CDS | 55.0% | |
| GCAACATGAAGGCCGGGTGA+TGG | - | chr2.4:8983266-8983285 | MS.gene31359:CDS | 60.0% | |
| GCAATGTGAAGGACGGCTGG+AGG | - | chr2.4:8982888-8982907 | MS.gene31359:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.4 | gene | 8981953 | 8983455 | 8981953 | ID=MS.gene31359 |
| chr2.4 | mRNA | 8981953 | 8983455 | 8981953 | ID=MS.gene31359.t1;Parent=MS.gene31359 |
| chr2.4 | exon | 8981953 | 8983455 | 8981953 | ID=MS.gene31359.t1.exon1;Parent=MS.gene31359.t1 |
| chr2.4 | CDS | 8981953 | 8983455 | 8981953 | ID=cds.MS.gene31359.t1;Parent=MS.gene31359.t1 |
| Gene Sequence |
| Protein sequence |
