AlfalfaGEDB | MS.gene057395

M. sativa cultivar XinJiangDaYe / MS.gene057395

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene057395.t1	XP_003595315.1	94.2	86	5	0	4	89	267	352	6.70E-37	162.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene057395.t1	G7IGG4	94.2	86	5	0	4	89	267	352	4.8e-37	162.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050438	MS.gene057395	0.800069	1.71E-48	-1.69E-46
MS.gene050680	MS.gene057395	0.832916	7.19E-56	-1.69E-46
MS.gene050780	MS.gene057395	0.812197	4.79E-51	-1.69E-46
MS.gene052426	MS.gene057395	0.816987	4.17E-52	-1.69E-46
MS.gene053074	MS.gene057395	0.828305	9.68E-55	-1.69E-46
MS.gene053166	MS.gene057395	0.804069	2.58E-49	-1.69E-46
MS.gene054433	MS.gene057395	0.80724	5.57E-50	-1.69E-46
MS.gene054559	MS.gene057395	0.801156	1.03E-48	-1.69E-46
MS.gene05510	MS.gene057395	0.824084	9.78E-54	-1.69E-46
MS.gene055358	MS.gene057395	0.818734	1.68E-52	-1.69E-46
MS.gene055701	MS.gene057395	0.80187	7.34E-49	-1.69E-46
MS.gene057394	MS.gene057395	0.826182	3.12E-54	-1.69E-46
MS.gene057395	MS.gene059128	0.831557	1.56E-55	-1.69E-46
MS.gene057395	MS.gene060322	0.816267	6.04E-52	-1.69E-46
MS.gene057395	MS.gene060998	0.805195	1.50E-49	-1.69E-46
MS.gene057395	MS.gene061333	0.816569	5.17E-52	-1.69E-46
MS.gene057395	MS.gene061334	0.816306	5.92E-52	-1.69E-46
MS.gene057395	MS.gene064524	0.811905	5.54E-51	-1.69E-46
MS.gene057395	MS.gene064944	0.808212	3.46E-50	-1.69E-46
MS.gene057395	MS.gene067305	0.811015	8.65E-51	-1.69E-46
MS.gene057395	MS.gene067566	0.834074	3.70E-56	-1.69E-46
MS.gene057395	MS.gene07115	0.824037	1.00E-53	-1.69E-46
MS.gene057395	MS.gene072671	0.80048	1.41E-48	-1.69E-46
MS.gene057395	MS.gene07344	0.812658	3.79E-51	-1.69E-46
MS.gene057395	MS.gene073460	0.812398	4.33E-51	-1.69E-46
MS.gene057395	MS.gene07942	0.803091	4.11E-49	-1.69E-46
MS.gene057395	MS.gene20635	0.816374	5.71E-52	-1.69E-46
MS.gene057395	MS.gene21911	0.822617	2.15E-53	-1.69E-46
MS.gene057395	MS.gene22731	0.830836	2.35E-55	-1.69E-46
MS.gene057395	MS.gene25868	0.833443	5.32E-56	-1.69E-46
MS.gene057395	MS.gene26514	0.811261	7.65E-51	-1.69E-46
MS.gene057395	MS.gene27008	0.801409	9.13E-49	-1.69E-46
MS.gene057395	MS.gene28498	0.808049	3.75E-50	-1.69E-46
MS.gene057395	MS.gene31359	0.804816	1.80E-49	-1.69E-46
MS.gene057395	MS.gene31609	0.803181	3.94E-49	-1.69E-46
MS.gene057395	MS.gene35428	0.801617	8.27E-49	-1.69E-46
MS.gene057395	MS.gene39638	0.810124	1.35E-50	-1.69E-46
MS.gene057395	MS.gene40837	-0.811156	8.06E-51	-1.69E-46
MS.gene057395	MS.gene45090	0.805845	1.10E-49	-1.69E-46
MS.gene057395	MS.gene48577	0.81716	3.81E-52	-1.69E-46
MS.gene057395	MS.gene49486	0.800663	1.30E-48	-1.69E-46
MS.gene057395	MS.gene49940	0.812843	3.46E-51	-1.69E-46
MS.gene057395	MS.gene53586	0.817801	2.73E-52	-1.69E-46
MS.gene057395	MS.gene56053	0.81307	3.08E-51	-1.69E-46
MS.gene057395	MS.gene56431	0.837461	5.13E-57	-1.69E-46
MS.gene057395	MS.gene57469	0.811619	6.40E-51	-1.69E-46
MS.gene057395	MS.gene60206	0.818305	2.10E-52	-1.69E-46
MS.gene057395	MS.gene61139	0.812263	4.63E-51	-1.69E-46
MS.gene057395	MS.gene61435	0.802347	5.86E-49	-1.69E-46
MS.gene057395	MS.gene66964	0.825425	4.72E-54	-1.69E-46
MS.gene057395	MS.gene68923	0.830724	2.50E-55	-1.69E-46
MS.gene057395	MS.gene69794	0.833777	4.39E-56	-1.69E-46
MS.gene057395	MS.gene70021	0.826237	3.03E-54	-1.69E-46
MS.gene057395	MS.gene70022	0.803975	2.70E-49	-1.69E-46
MS.gene057395	MS.gene72268	0.825798	3.85E-54	-1.69E-46
MS.gene057395	MS.gene75321	0.803905	2.79E-49	-1.69E-46
MS.gene057395	MS.gene75490	0.806174	9.35E-50	-1.69E-46
MS.gene057395	MS.gene82810	0.811874	5.63E-51	-1.69E-46
MS.gene057395	MS.gene84326	0.80166	8.11E-49	-1.69E-46
MS.gene057395	MS.gene84833	0.808159	3.55E-50	-1.69E-46
MS.gene057395	MS.gene87264	0.820585	6.35E-53	-1.69E-46
MS.gene057395	MS.gene87804	0.807605	4.66E-50	-1.69E-46
MS.gene057395	MS.gene87987	0.815648	8.30E-52	-1.69E-46
MS.gene057395	MS.gene88960	0.804026	2.63E-49	-1.69E-46
MS.gene057395	MS.gene91807	0.810037	1.41E-50	-1.69E-46
MS.gene057395	MS.gene91809	0.801847	7.42E-49	-1.69E-46
MS.gene057395	MS.gene93888	0.819256	1.28E-52	-1.69E-46
MS.gene057395	MS.gene93890	0.801162	1.03E-48	-1.69E-46
MS.gene057395	MS.gene95377	0.806739	7.11E-50	-1.69E-46
MS.gene057395	MS.gene96503	0.815036	1.14E-51	-1.69E-46
MS.gene057395	MS.gene98543	0.809048	2.29E-50	-1.69E-46
MS.gene057395	MS.gene99735	0.815773	7.78E-52	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene057395.t1	MTR_2g042420	94.186	86	5	0	4	89	267	352	2.87e-52	166
MS.gene057395.t1	MTR_2g042430	74.419	86	22	0	4	89	286	371	2.36e-40	136
MS.gene057395.t1	MTR_2g042370	74.419	86	22	0	4	89	275	360	5.43e-40	135
MS.gene057395.t1	MTR_2g039370	68.519	54	12	1	36	89	1	49	6.23e-17	73.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 13 sgRNAs with CRISPR-Local

Find 31 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AATAAAGCTACGAAGTGTTT+TGG	0.341284	2.1:-50735001	None:intergenic
GTCTTAATACTGACTTTGTT+GGG	0.387123	2.1:+50735185	MS.gene057395:CDS
CGTAGCTTTATTTCCAAGGC+TGG	0.429510	2.1:+50735012	MS.gene057395:CDS
TTAATCATGTTAACCAGCCT+TGG	0.434131	2.1:-50735025	None:intergenic
AGTCTTAATACTGACTTTGT+TGG	0.447028	2.1:+50735184	MS.gene057395:CDS
TTTGAGAACAATCTTTACTT+AGG	0.457589	2.1:+50735160	MS.gene057395:CDS
AAAAGTTTGGTGATAATGAT+GGG	0.521526	2.1:+50735104	MS.gene057395:CDS
ACTTCGTAGCTTTATTTCCA+AGG	0.527057	2.1:+50735008	MS.gene057395:CDS
TAGTTCTATGCACTTTGCAG+TGG	0.550550	2.1:-50735214	None:intergenic
TAGTGTAACTAAATCTGCAA+TGG	0.584002	2.1:+50735048	MS.gene057395:CDS
TGTAACTAAATCTGCAATGG+TGG	0.654164	2.1:+50735051	MS.gene057395:CDS
GTGGTGAAAGTGACAACTGA+AGG	0.697705	2.1:+50735070	MS.gene057395:CDS
CCCGATTCACAATGACTGTG+AGG	0.719070	2.1:+50734583	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	ATTTATATTGAAATTTTGAT+TGG	+	chr2.1:50734870-50734889	MS.gene057395:intron	10.0%
!!	TATATATTAATTGATGTTAC+AGG	+	chr2.1:50734937-50734956	MS.gene057395:intron	15.0%
!!	TAATTTCATCAATAACTTCA+AGG	+	chr2.1:50734610-50734629	MS.gene057395:intron	20.0%
!	GGCAATATTATCAAAAAGTT+TGG	+	chr2.1:50735091-50735110	MS.gene057395:CDS	25.0%
!	GTAAATTAGAGACGATTAAA+AGG	+	chr2.1:50734639-50734658	MS.gene057395:intron	25.0%
!	TTTGAGAACAATCTTTACTT+AGG	+	chr2.1:50735160-50735179	MS.gene057395:CDS	25.0%
!!	AAAAAGTTTGGTGATAATGA+TGG	+	chr2.1:50735103-50735122	MS.gene057395:CDS	25.0%
!!	AAAAGTTTGGTGATAATGAT+GGG	+	chr2.1:50735104-50735123	MS.gene057395:CDS	25.0%
	ATTGGTCATGTTATGCAATA+AGG	+	chr2.1:50734888-50734907	MS.gene057395:intron	30.0%
	TAGTGTAACTAAATCTGCAA+TGG	+	chr2.1:50735048-50735067	MS.gene057395:CDS	30.0%
!	TTTCAATCTGAAGTTTCGAT+TGG	+	chr2.1:50734834-50734853	MS.gene057395:intron	30.0%
!!	AATAAAGCTACGAAGTGTTT+TGG	-	chr2.1:50735004-50735023	None:intergenic	30.0%
!!	AGTCTTAATACTGACTTTGT+TGG	+	chr2.1:50735184-50735203	MS.gene057395:CDS	30.0%
!!	GTCTTAATACTGACTTTGTT+GGG	+	chr2.1:50735185-50735204	MS.gene057395:CDS	30.0%
	TGTAACTAAATCTGCAATGG+TGG	+	chr2.1:50735051-50735070	MS.gene057395:CDS	35.0%
	TTAATCATGTTAACCAGCCT+TGG	-	chr2.1:50735028-50735047	None:intergenic	35.0%
!!	ACTTCGTAGCTTTATTTCCA+AGG	+	chr2.1:50735008-50735027	MS.gene057395:CDS	35.0%
!!!	GAAGTGTTTTGGTACTTTAG+AGG	-	chr2.1:50734993-50735012	None:intergenic	35.0%
	GAAAATACCCACATGTGTGT+CGG	+	chr2.1:50734792-50734811	MS.gene057395:intron	40.0%
	TGTTACAGGTGACTTCTGAA+AGG	+	chr2.1:50734951-50734970	MS.gene057395:intron	40.0%
	TTTGACGTTCGTATCTGTGT+CGG	-	chr2.1:50734719-50734738	None:intergenic	40.0%
!	CAATCTGAAGTTTCGATTGG+CGG	+	chr2.1:50734837-50734856	MS.gene057395:intron	40.0%
	GTGGTGAAAGTGACAACTGA+AGG	+	chr2.1:50735070-50735089	MS.gene057395:CDS	45.0%
!	ACAGGTGACTTCTGAAAGGA+TGG	+	chr2.1:50734955-50734974	MS.gene057395:intron	45.0%
!	AGGTGACTTCTGAAAGGATG+GGG	+	chr2.1:50734957-50734976	MS.gene057395:intron	45.0%
!	CAGGTGACTTCTGAAAGGAT+GGG	+	chr2.1:50734956-50734975	MS.gene057395:intron	45.0%
!!	CGTAGCTTTATTTCCAAGGC+TGG	+	chr2.1:50735012-50735031	MS.gene057395:CDS	45.0%
	TCCGACACCGACACACATGT+GGG	-	chr2.1:50734802-50734821	None:intergenic	55.0%
!	ACCCACATGTGTGTCGGTGT+CGG	+	chr2.1:50734798-50734817	MS.gene057395:intron	55.0%
	GTCCGACACCGACACACATG+TGG	-	chr2.1:50734803-50734822	None:intergenic	60.0%
!!	TGTGTGTCGGTGTCGGACAC+TGG	+	chr2.1:50734805-50734824	MS.gene057395:intron	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.1	gene	50734594	50735231	50734594	ID=MS.gene057395
chr2.1	mRNA	50734594	50735231	50734594	ID=MS.gene057395.t1;Parent=MS.gene057395
chr2.1	exon	50734594	50734604	50734594	ID=MS.gene057395.t1.exon1;Parent=MS.gene057395.t1
chr2.1	CDS	50734594	50734604	50734594	ID=cds.MS.gene057395.t1;Parent=MS.gene057395.t1
chr2.1	exon	50734973	50735231	50734973	ID=MS.gene057395.t1.exon2;Parent=MS.gene057395.t1
chr2.1	CDS	50734973	50735231	50734973	ID=cds.MS.gene057395.t1;Parent=MS.gene057395.t1

Gene Sequence

>MS.gene057395

ATGACTGTGAGGTATCTAATTTCATCAATAACTTCAAGGTAACAAGTAAATTAGAGACGATTAAAAGGACTTTGTTTTTTCTTCTCTCATTCAAGTGTAATTTTAGTTTTATATGAGAAGCACCGACACAGATACGAACGTCAAATACGAAGCTGACAAATCGACTGATATGTAATAATTTGATAGTATTGAATAATTGAAAATACCCACATGTGTGTCGGTGTCGGACACTGGACATGCTTTCAATCTGAAGTTTCGATTGGCGGTGTTATATAGATTTATATTGAAATTTTGATTGGTCATGTTATGCAATAAGGCATGATGATGAATTGTTTATCTTGAATATATATTAATTGATGTTACAGGTGACTTCTGAAAGGATGGGGTTTCTGCACACCTCTAAAGTACCAAAACACTTCGTAGCTTTATTTCCAAGGCTGGTTAACATGATTAATAGTGTAACTAAATCTGCAATGGTGGTGAAAGTGACAACTGAAGGCAATATTATCAAAAAGTTTGGTGATAATGATGGGAAGAAAATTACTTTTGTAACTTCAGCTGTTGAGTTTGAGAACAATCTTTACTTAGGTAGTCTTAATACTGACTTTGTTGGGAAATTTCCACTGCAAAGTGCATAG

Protein sequence

>MS.gene057395.t1

MTVRMGFLHTSKVPKHFVALFPRLVNMINSVTKSAMVVKVTTEGNIIKKFGDNDGKKITFVTSAVEFENNLYLGSLNTDFVGKFPLQSA