Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33498.t1 | XP_003604650.1 | 97.6 | 123 | 3 | 0 | 1 | 123 | 1 | 123 | 7.50E-55 | 223 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33498.t1 | O80439 | 45.4 | 119 | 63 | 1 | 5 | 123 | 2 | 118 | 4.2e-14 | 79.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33498.t1 | G7JN21 | 97.6 | 123 | 3 | 0 | 1 | 123 | 1 | 123 | 5.4e-55 | 223.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049171 | MS.gene33498 | 0.817506 | 3.18E-52 | -1.69E-46 |
| MS.gene049173 | MS.gene33498 | 0.855194 | 7.43E-62 | -1.69E-46 |
| MS.gene049285 | MS.gene33498 | 0.82221 | 2.68E-53 | -1.69E-46 |
| MS.gene050163 | MS.gene33498 | 0.80491 | 1.72E-49 | -1.69E-46 |
| MS.gene050165 | MS.gene33498 | 0.811307 | 7.48E-51 | -1.69E-46 |
| MS.gene050333 | MS.gene33498 | 0.804103 | 2.54E-49 | -1.69E-46 |
| MS.gene050504 | MS.gene33498 | 0.801695 | 7.98E-49 | -1.69E-46 |
| MS.gene05100 | MS.gene33498 | 0.822284 | 2.57E-53 | -1.69E-46 |
| MS.gene051949 | MS.gene33498 | 0.810449 | 1.15E-50 | -1.69E-46 |
| MS.gene054642 | MS.gene33498 | 0.815031 | 1.14E-51 | -1.69E-46 |
| MS.gene054954 | MS.gene33498 | 0.813907 | 2.02E-51 | -1.69E-46 |
| MS.gene055362 | MS.gene33498 | 0.810351 | 1.20E-50 | -1.69E-46 |
| MS.gene056657 | MS.gene33498 | 0.805303 | 1.42E-49 | -1.69E-46 |
| MS.gene058419 | MS.gene33498 | 0.814306 | 1.65E-51 | -1.69E-46 |
| MS.gene059022 | MS.gene33498 | 0.823252 | 1.53E-53 | -1.69E-46 |
| MS.gene05912 | MS.gene33498 | 0.813177 | 2.92E-51 | -1.69E-46 |
| MS.gene059677 | MS.gene33498 | 0.817142 | 3.84E-52 | -1.69E-46 |
| MS.gene060337 | MS.gene33498 | -0.802437 | 5.61E-49 | -1.69E-46 |
| MS.gene060703 | MS.gene33498 | 0.839768 | 1.30E-57 | -1.69E-46 |
| MS.gene060762 | MS.gene33498 | 0.80087 | 1.18E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33498.t1 | MTR_4g015540 | 97.561 | 123 | 3 | 0 | 1 | 123 | 1 | 123 | 1.26e-83 | 240 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33498.t1 | AT2G38140 | 50.420 | 119 | 57 | 1 | 5 | 123 | 2 | 118 | 3.93e-26 | 94.7 |
Find 39 sgRNAs with CRISPR-Local
Find 68 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATCTTCAAGCTTCTCTTTCT+TGG | 0.182199 | 4.2:+85578225 | None:intergenic |
| TCGCTTTCACCAACCCCTTC+TGG | 0.306084 | 4.2:-85579128 | MS.gene33498:intron |
| GTTCCCTTGTTCTTGTTCCT+TGG | 0.334623 | 4.2:+85578283 | None:intergenic |
| TTCTCTTTCTTGGTAGCATC+TGG | 0.349140 | 4.2:+85578235 | None:intergenic |
| GAAAGAGAAGCTTGAAGATA+AGG | 0.394365 | 4.2:-85578221 | MS.gene33498:CDS |
| AAACGGTTCAGCCATTCGTT+TGG | 0.400874 | 4.2:-85578961 | MS.gene33498:CDS |
| TGTATTGTGGCAGAGGTGAT+AGG | 0.423136 | 4.2:-85578999 | MS.gene33498:CDS |
| AGGAACAAGAACAAGGGAAC+AGG | 0.442127 | 4.2:-85578280 | MS.gene33498:CDS |
| GCGTGGAGTTGAAGAGATAG+TGG | 0.472152 | 4.2:+85579239 | None:intergenic |
| CTTGTAGTGTATTGTGGCAG+AGG | 0.475393 | 4.2:-85579006 | MS.gene33498:intron |
| GATTGGTGAGTGAAGAGATT+GGG | 0.488179 | 4.2:+85579187 | None:intergenic |
| TCTTTCTTGGTAGCATCTGG+TGG | 0.499433 | 4.2:+85578238 | None:intergenic |
| AAAGATGAGAAGAGGAAGCG+TGG | 0.518100 | 4.2:+85579222 | None:intergenic |
| GGAAAACTGCTAAGGGGAAA+CGG | 0.521583 | 4.2:-85578978 | MS.gene33498:CDS |
| AAGAGATTGGGATTGAGAGA+AGG | 0.529493 | 4.2:+85579199 | None:intergenic |
| AAATCAACTAACTAGAAAAG+AGG | 0.532100 | 4.2:+85578170 | None:intergenic |
| TGATTGGTGAGTGAAGAGAT+TGG | 0.539355 | 4.2:+85579186 | None:intergenic |
| AGGTGATAGGAAAACTGCTA+AGG | 0.552164 | 4.2:-85578986 | MS.gene33498:CDS |
| TCTGGTGGAGCATAAATCCT+TGG | 0.553001 | 4.2:+85578253 | None:intergenic |
| ACAAGGTTGAAGAGAGGGTG+GGG | 0.553508 | 4.2:+85579150 | None:intergenic |
| GGGAATGATGAAGTGATGAT+TGG | 0.557778 | 4.2:+85579170 | None:intergenic |
| AGAGAAGGAAAGATGAGAAG+AGG | 0.560854 | 4.2:+85579214 | None:intergenic |
| AATCAACTAACTAGAAAAGA+GGG | 0.565948 | 4.2:+85578171 | None:intergenic |
| GGTGATAGGAAAACTGCTAA+GGG | 0.567207 | 4.2:-85578985 | MS.gene33498:CDS |
| ACATTACATTTCCAAACGAA+TGG | 0.569487 | 4.2:+85578950 | None:intergenic |
| AAGGCCAAGGAACAAGAACA+AGG | 0.570532 | 4.2:-85578287 | MS.gene33498:CDS |
| CGACAAGGTTGAAGAGAGGG+TGG | 0.579098 | 4.2:+85579148 | None:intergenic |
| GGTGGAGCATAAATCCTTGG+TGG | 0.582114 | 4.2:+85578256 | None:intergenic |
| TATCCTCTCAGTTATGGCCA+TGG | 0.592654 | 4.2:-85579291 | None:intergenic |
| GACAAGGTTGAAGAGAGGGT+GGG | 0.600630 | 4.2:+85579149 | None:intergenic |
| GATGTTATCAGGCAAGGCCA+AGG | 0.622878 | 4.2:-85578300 | MS.gene33498:intron |
| AAGCGACAAGGTTGAAGAGA+GGG | 0.625118 | 4.2:+85579145 | None:intergenic |
| AGTTGAAGAGATAGTGGTGA+AGG | 0.628052 | 4.2:+85579245 | None:intergenic |
| AGGCCAAGGAACAAGAACAA+GGG | 0.651122 | 4.2:-85578286 | MS.gene33498:CDS |
| TAGAAGCAGTGATGATGCCA+TGG | 0.657767 | 4.2:+85579274 | None:intergenic |
| AAAGCGACAAGGTTGAAGAG+AGG | 0.661146 | 4.2:+85579144 | None:intergenic |
| AGGGGTTGGTGAAAGCGACA+AGG | 0.666764 | 4.2:+85579133 | None:intergenic |
| ACAAGGGAACAGGACCACCA+AGG | 0.709490 | 4.2:-85578270 | MS.gene33498:CDS |
| GTGATAGGAAAACTGCTAAG+GGG | 0.715035 | 4.2:-85578984 | MS.gene33498:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GTAATTTTTTCTTTAAAAAA+TGG | - | chr4.2:85578349-85578368 | MS.gene33498:intron | 10.0% |
| !! | AACGAACCAAAATAAAAAAA+TGG | + | chr4.2:85578420-85578439 | None:intergenic | 20.0% |
| !! | AATGTACATTAATTCATGTT+TGG | - | chr4.2:85578663-85578682 | MS.gene33498:intron | 20.0% |
| !! | ACGAACCAAAATAAAAAAAT+GGG | + | chr4.2:85578419-85578438 | None:intergenic | 20.0% |
| !! | TAAAGAAAAAATTACCAGAA+GGG | + | chr4.2:85578344-85578363 | None:intergenic | 20.0% |
| !! | TACAAAATAACATTAGAACT+AGG | + | chr4.2:85579122-85579141 | None:intergenic | 20.0% |
| !! | TTAAAGAAAAAATTACCAGA+AGG | + | chr4.2:85578345-85578364 | None:intergenic | 20.0% |
| !! | TTCAAAAGTGGTTATAAAAT+AGG | - | chr4.2:85578817-85578836 | MS.gene33498:intron | 20.0% |
| !!! | ATATGTTATTTGATGTTATC+AGG | - | chr4.2:85579144-85579163 | MS.gene33498:CDS | 20.0% |
| !!! | ATCATTATTATTGTTTGTTG+AGG | - | chr4.2:85579061-85579080 | MS.gene33498:intron | 20.0% |
| !!! | ATGATGTATCCTTTTATAAA+AGG | - | chr4.2:85578736-85578755 | MS.gene33498:intron | 20.0% |
| !!! | GATAACCCATTTTTTTATTT+TGG | - | chr4.2:85578411-85578430 | MS.gene33498:intron | 20.0% |
| !!! | GATTTTTGTTGAAATTTTTG+TGG | - | chr4.2:85579083-85579102 | MS.gene33498:intron | 20.0% |
| !!! | TGGAATTTTTGTTATGAATT+TGG | - | chr4.2:85578369-85578388 | MS.gene33498:intron | 20.0% |
| ! | AAAGAAAAAATTACCAGAAG+GGG | + | chr4.2:85578343-85578362 | None:intergenic | 25.0% |
| ! | CAAAATTCGAGATTATTTCA+TGG | - | chr4.2:85579024-85579043 | MS.gene33498:intron | 25.0% |
| ! | GAATTTATTGAGTGTATGTT+CGG | - | chr4.2:85578569-85578588 | MS.gene33498:intron | 25.0% |
| !!! | CATGTAGTTTTCTAATTTGT+AGG | - | chr4.2:85578972-85578991 | MS.gene33498:CDS | 25.0% |
| !!! | TGGTAGTTATTTTTCAAAAG+TGG | - | chr4.2:85578805-85578824 | MS.gene33498:intron | 25.0% |
| ACATTACATTTCCAAACGAA+TGG | + | chr4.2:85578508-85578527 | None:intergenic | 30.0% | |
| ATCCATTTAACTCATGTTTC+TGG | - | chr4.2:85578905-85578924 | MS.gene33498:intron | 30.0% | |
| GGTTATAAAATAGGCTTACA+TGG | - | chr4.2:85578826-85578845 | MS.gene33498:intron | 30.0% | |
| TTATTTGATGTTATCAGGCA+AGG | - | chr4.2:85579149-85579168 | MS.gene33498:CDS | 30.0% | |
| ! | GATGCGTATCCTTTTATAAA+AGG | + | chr4.2:85578748-85578767 | None:intergenic | 30.0% |
| ! | TGTTTTCTTGTAGTGTATTG+TGG | - | chr4.2:85578443-85578462 | MS.gene33498:intron | 30.0% |
| !!! | GATTTTTTAGTCCATGAAGA+GGG | - | chr4.2:85578941-85578960 | MS.gene33498:intron | 30.0% |
| !!! | TGATTTTTTAGTCCATGAAG+AGG | - | chr4.2:85578940-85578959 | MS.gene33498:intron | 30.0% |
| AAAAAATTACCAGAAGGGGT+TGG | + | chr4.2:85578339-85578358 | None:intergenic | 35.0% | |
| ATCTTCAAGCTTCTCTTTCT+TGG | + | chr4.2:85579233-85579252 | None:intergenic | 35.0% | |
| GAAAGAGAAGCTTGAAGATA+AGG | - | chr4.2:85579234-85579253 | MS.gene33498:CDS | 35.0% | |
| GACCAGAAACATGAGTTAAA+TGG | + | chr4.2:85578910-85578929 | None:intergenic | 35.0% | |
| TATTGAGTGTATGTTCGGTT+AGG | - | chr4.2:85578574-85578593 | MS.gene33498:intron | 35.0% | |
| !!! | ATGTTTTTAGTCCCTCTTCA+TGG | + | chr4.2:85578955-85578974 | None:intergenic | 35.0% |
| AACTACCAAATGCTTCATGC+AGG | + | chr4.2:85578793-85578812 | None:intergenic | 40.0% | |
| AAGAGATTGGGATTGAGAGA+AGG | + | chr4.2:85578259-85578278 | None:intergenic | 40.0% | |
| AGAGAAGGAAAGATGAGAAG+AGG | + | chr4.2:85578244-85578263 | None:intergenic | 40.0% | |
| AGGTGATAGGAAAACTGCTA+AGG | - | chr4.2:85578469-85578488 | MS.gene33498:intron | 40.0% | |
| GATTGGTGAGTGAAGAGATT+GGG | + | chr4.2:85578271-85578290 | None:intergenic | 40.0% | |
| GGGAATGATGAAGTGATGAT+TGG | + | chr4.2:85578288-85578307 | None:intergenic | 40.0% | |
| GGTGATAGGAAAACTGCTAA+GGG | - | chr4.2:85578470-85578489 | MS.gene33498:intron | 40.0% | |
| GTGATAGGAAAACTGCTAAG+GGG | - | chr4.2:85578471-85578490 | MS.gene33498:intron | 40.0% | |
| TGATTGGTGAGTGAAGAGAT+TGG | + | chr4.2:85578272-85578291 | None:intergenic | 40.0% | |
| ! | AGTTGAAGAGATAGTGGTGA+AGG | + | chr4.2:85578213-85578232 | None:intergenic | 40.0% |
| ! | TTACACCTGCATGAAGCATT+TGG | - | chr4.2:85578785-85578804 | MS.gene33498:intron | 40.0% |
| ! | TTCTCTTTCTTGGTAGCATC+TGG | + | chr4.2:85579223-85579242 | None:intergenic | 40.0% |
| AAACGGTTCAGCCATTCGTT+TGG | - | chr4.2:85578494-85578513 | MS.gene33498:intron | 45.0% | |
| AAAGATGAGAAGAGGAAGCG+TGG | + | chr4.2:85578236-85578255 | None:intergenic | 45.0% | |
| AAAGCGACAAGGTTGAAGAG+AGG | + | chr4.2:85578314-85578333 | None:intergenic | 45.0% | |
| AAGCGACAAGGTTGAAGAGA+GGG | + | chr4.2:85578313-85578332 | None:intergenic | 45.0% | |
| AAGGCCAAGGAACAAGAACA+AGG | - | chr4.2:85579168-85579187 | MS.gene33498:CDS | 45.0% | |
| AGGAACAAGAACAAGGGAAC+AGG | - | chr4.2:85579175-85579194 | MS.gene33498:CDS | 45.0% | |
| AGGCCAAGGAACAAGAACAA+GGG | - | chr4.2:85579169-85579188 | MS.gene33498:CDS | 45.0% | |
| CTTGTAGTGTATTGTGGCAG+AGG | - | chr4.2:85578449-85578468 | MS.gene33498:intron | 45.0% | |
| GGAAAACTGCTAAGGGGAAA+CGG | - | chr4.2:85578477-85578496 | MS.gene33498:intron | 45.0% | |
| GTTCCCTTGTTCTTGTTCCT+TGG | + | chr4.2:85579175-85579194 | None:intergenic | 45.0% | |
| TCTGGTGGAGCATAAATCCT+TGG | + | chr4.2:85579205-85579224 | None:intergenic | 45.0% | |
| TGTATTGTGGCAGAGGTGAT+AGG | - | chr4.2:85578456-85578475 | MS.gene33498:intron | 45.0% | |
| ! | TAGAAGCAGTGATGATGCCA+TGG | + | chr4.2:85578184-85578203 | None:intergenic | 45.0% |
| ! | TCTTTCTTGGTAGCATCTGG+TGG | + | chr4.2:85579220-85579239 | None:intergenic | 45.0% |
| ACAAGGTTGAAGAGAGGGTG+GGG | + | chr4.2:85578308-85578327 | None:intergenic | 50.0% | |
| GACAAGGTTGAAGAGAGGGT+GGG | + | chr4.2:85578309-85578328 | None:intergenic | 50.0% | |
| GATGTTATCAGGCAAGGCCA+AGG | - | chr4.2:85579155-85579174 | MS.gene33498:CDS | 50.0% | |
| GCGTGGAGTTGAAGAGATAG+TGG | + | chr4.2:85578219-85578238 | None:intergenic | 50.0% | |
| GGTGGAGCATAAATCCTTGG+TGG | + | chr4.2:85579202-85579221 | None:intergenic | 50.0% | |
| ACAAGGGAACAGGACCACCA+AGG | - | chr4.2:85579185-85579204 | MS.gene33498:CDS | 55.0% | |
| CGACAAGGTTGAAGAGAGGG+TGG | + | chr4.2:85578310-85578329 | None:intergenic | 55.0% | |
| TCGCTTTCACCAACCCCTTC+TGG | - | chr4.2:85578327-85578346 | MS.gene33498:intron | 55.0% | |
| !! | AGGGGTTGGTGAAAGCGACA+AGG | + | chr4.2:85578325-85578344 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 85578177 | 85579300 | 85578177 | ID=MS.gene33498 |
| chr4.2 | mRNA | 85578177 | 85579300 | 85578177 | ID=MS.gene33498.t1;Parent=MS.gene33498 |
| chr4.2 | exon | 85579129 | 85579300 | 85579129 | ID=MS.gene33498.t1.exon1;Parent=MS.gene33498.t1 |
| chr4.2 | CDS | 85579129 | 85579300 | 85579129 | ID=cds.MS.gene33498.t1;Parent=MS.gene33498.t1 |
| chr4.2 | exon | 85578957 | 85579021 | 85578957 | ID=MS.gene33498.t1.exon2;Parent=MS.gene33498.t1 |
| chr4.2 | CDS | 85578957 | 85579021 | 85578957 | ID=cds.MS.gene33498.t1;Parent=MS.gene33498.t1 |
| chr4.2 | exon | 85578177 | 85578311 | 85578177 | ID=MS.gene33498.t1.exon3;Parent=MS.gene33498.t1 |
| chr4.2 | CDS | 85578177 | 85578311 | 85578177 | ID=cds.MS.gene33498.t1;Parent=MS.gene33498.t1 |
| Gene Sequence |
| Protein sequence |
