AlfalfaGEDB | MS.gene34393

M. sativa cultivar XinJiangDaYe / MS.gene34393

KEGG: -
GO: -
PFAM: PF00504.24

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene34393.t1	XP_013469748.1	86.1	201	24	1	1	201	1	197	3.70E-83	317.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene34393.t1	P11432	77.6	201	40	1	1	201	1	196	1.5e-77	290.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene34393.t1	A0A072VQ49	86.1	201	24	1	1	201	1	197	2.7e-83	317.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene05081	MS.gene34393	0.807985	3.87E-50	-1.69E-46
MS.gene051734	MS.gene34393	0.840352	9.16E-58	-1.69E-46
MS.gene051838	MS.gene34393	0.830405	2.99E-55	-1.69E-46
MS.gene051941	MS.gene34393	0.817438	3.30E-52	-1.69E-46
MS.gene052414	MS.gene34393	0.811257	7.67E-51	-1.69E-46
MS.gene052469	MS.gene34393	0.811904	5.54E-51	-1.69E-46
MS.gene052870	MS.gene34393	0.830999	2.14E-55	-1.69E-46
MS.gene052872	MS.gene34393	0.83569	1.45E-56	-1.69E-46
MS.gene053824	MS.gene34393	0.840452	8.62E-58	-1.69E-46
MS.gene054884	MS.gene34393	-0.822245	2.63E-53	-1.69E-46
MS.gene05494	MS.gene34393	0.824488	7.86E-54	-1.69E-46
MS.gene055224	MS.gene34393	0.807498	4.91E-50	-1.69E-46
MS.gene055539	MS.gene34393	0.830019	3.72E-55	-1.69E-46
MS.gene055741	MS.gene34393	0.829613	4.67E-55	-1.69E-46
MS.gene055744	MS.gene34393	0.827231	1.75E-54	-1.69E-46
MS.gene05589	MS.gene34393	0.819934	8.95E-53	-1.69E-46
MS.gene056283	MS.gene34393	0.835951	1.24E-56	-1.69E-46
MS.gene056387	MS.gene34393	0.830337	3.11E-55	-1.69E-46
MS.gene057019	MS.gene34393	0.803297	3.73E-49	-1.69E-46
MS.gene057076	MS.gene34393	0.803944	2.74E-49	-1.69E-46
MS.gene058448	MS.gene34393	0.832607	8.58E-56	-1.69E-46
MS.gene058838	MS.gene34393	0.808239	3.42E-50	-1.69E-46
MS.gene059023	MS.gene34393	0.807982	3.87E-50	-1.69E-46
MS.gene059717	MS.gene34393	0.838408	2.93E-57	-1.69E-46
MS.gene060835	MS.gene34393	0.802566	5.28E-49	-1.69E-46
MS.gene060982	MS.gene34393	0.803919	2.77E-49	-1.69E-46
MS.gene061231	MS.gene34393	0.84623	2.47E-59	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene34393.t1	MTR_1g102570	89.011	182	16	1	20	201	21	198	7.49e-108	307
MS.gene34393.t1	MTR_1g102800	89.011	182	16	1	20	201	20	197	1.63e-107	306
MS.gene34393.t1	MTR_1g102780	89.560	182	17	1	20	201	21	200	1.29e-101	292
MS.gene34393.t1	MTR_1g102810	74.595	185	31	2	17	201	14	182	8.80e-94	271
MS.gene34393.t1	MTR_1g102830	76.374	182	27	2	20	201	21	186	1.14e-93	271

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene34393.t1	AT3G22840	51.724	203	88	3	1	201	1	195	4.79e-62	191
MS.gene34393.t1	AT4G14690	52.970	202	85	4	1	201	1	193	1.65e-58	182

Find 56 sgRNAs with CRISPR-Local

Find 75 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGCTCAATGAGCACCATTTC+TGG	0.210857	1.2:+76485568	MS.gene34393:CDS
TGGTGGCATCCCTTGGTTCT+TGG	0.234840	1.2:+76486178	MS.gene34393:CDS
GGTGGCATCCCTTGGTTCTT+GGG	0.263333	1.2:+76486179	MS.gene34393:CDS
GGTTTGTTTGATCAAATATA+TGG	0.294584	1.2:+76486155	MS.gene34393:CDS
GGTGATGATGATGGTGTAAT+TGG	0.299898	1.2:-76485845	None:intergenic
CAGAAGGGATGTTGCTGAAT+TGG	0.358772	1.2:-76485606	None:intergenic
TATGTTGGGTTTGATTGCTT+TGG	0.370659	1.2:+76486319	MS.gene34393:CDS
TGTGATGGTGGTGGTGGTGT+TGG	0.387566	1.2:-76485872	None:intergenic
GGCCTTTACAGAGTATGTTA+AGG	0.395379	1.2:+76486340	MS.gene34393:CDS
AATGGAAGGTTAGCTATGAT+TGG	0.400708	1.2:+76486092	MS.gene34393:CDS
GCCTTTACAGAGTATGTTAA+GGG	0.401917	1.2:+76486341	MS.gene34393:CDS
CCATTGATTCTCTCAGGTGC+TGG	0.411549	1.2:-76486074	None:intergenic
GGTGGTGGTGGTGTTGGTGT+TGG	0.414518	1.2:-76485866	None:intergenic
TGCCATCAAGTCCGAAAACT+TGG	0.420630	1.2:-76486042	None:intergenic
GCTGGTTGTGATGGTGGTGG+TGG	0.430893	1.2:-76485878	None:intergenic
AACCTTCCATTGATTCTCTC+AGG	0.448618	1.2:-76486080	None:intergenic
GACTCTAGACTTGCCAGAAA+TGG	0.452328	1.2:-76485581	None:intergenic
GTGGCATCCCTTGGTTCTTG+GGG	0.453269	1.2:+76486180	MS.gene34393:CDS
GAATGGAAGAATTGCTATGT+TGG	0.460944	1.2:+76486304	MS.gene34393:CDS
GTGATGATGATGGTGTAATT+GGG	0.461935	1.2:-76485844	None:intergenic
TACCTTTGGTGCGCGAGTAT+AGG	0.465801	1.2:-76485900	None:intergenic
GTTGGTGTTGGTGATGATGA+TGG	0.479914	1.2:-76485854	None:intergenic
TCGGACTTGATGGCATTTGG+TGG	0.487791	1.2:+76486050	MS.gene34393:CDS
AATGGAAGAATTGCTATGTT+GGG	0.499743	1.2:+76486305	MS.gene34393:CDS
CGGACTTGATGGCATTTGGT+GGG	0.500178	1.2:+76486051	MS.gene34393:CDS
CCAGCACCTGAGAGAATCAA+TGG	0.516939	1.2:+76486074	MS.gene34393:CDS
AGTGTTGAGTCCAAGTCAAA+AGG	0.522475	1.2:+76486254	MS.gene34393:CDS
TTTGGTGCGCGAGTATAGGC+TGG	0.530750	1.2:-76485896	None:intergenic
TAGGCTGGTTGTGATGGTGG+TGG	0.536961	1.2:-76485881	None:intergenic
TCAGATGCTGAGCTGTGGAA+TGG	0.542277	1.2:+76486287	MS.gene34393:CDS
AACCTTAATTACCTCAGCCA+TGG	0.545130	1.2:-76485674	None:intergenic
GGTGGAGATAGCAAATGGAC+AGG	0.547189	1.2:+76486133	MS.gene34393:CDS
ATAGCATTCCTTCTGAAGCT+AGG	0.551512	1.2:-76485637	None:intergenic
TATATGGTGGTGGCATCCCT+TGG	0.552085	1.2:+76486171	MS.gene34393:CDS
ACCCTTAACATACTCTGTAA+AGG	0.552111	1.2:-76486342	None:intergenic
CGAGTATAGGCTGGTTGTGA+TGG	0.554321	1.2:-76485887	None:intergenic
GATGGTGTAATTGGGTCTAC+AGG	0.568013	1.2:-76485836	None:intergenic
TTTGATCAAATATATGGTGG+TGG	0.569010	1.2:+76486161	MS.gene34393:CDS
AAGCTAGGCATGTACACAGA+AGG	0.579754	1.2:-76485622	None:intergenic
TGGACTCAACACTAACACCT+TGG	0.588832	1.2:-76486244	None:intergenic
AGCCTATACTCGCGCACCAA+AGG	0.589710	1.2:+76485898	MS.gene34393:CDS
TTGTTTGATCAAATATATGG+TGG	0.590396	1.2:+76486158	MS.gene34393:CDS
TGTCCTCAGATGCTGAGCTG+TGG	0.594735	1.2:+76486282	MS.gene34393:CDS
CACTTGTCCCCAAGAACCAA+GGG	0.598517	1.2:-76486187	None:intergenic
TATCTTGAAAGTTCGATCCA+TGG	0.599815	1.2:+76485657	MS.gene34393:CDS
GTATAGGCTGGTTGTGATGG+TGG	0.608859	1.2:-76485884	None:intergenic
AGCTAGGCATGTACACAGAA+GGG	0.614155	1.2:-76485621	None:intergenic
ATTCCACAGCTCAGCATCTG+AGG	0.616858	1.2:-76486285	None:intergenic
GAAAGTTCGATCCATGGCTG+AGG	0.622095	1.2:+76485663	MS.gene34393:CDS
TGTACATGCCTAGCTTCAGA+AGG	0.622254	1.2:+76485629	MS.gene34393:CDS
ACACTTGTCCCCAAGAACCA+AGG	0.642060	1.2:-76486188	None:intergenic
GTGGAGATAGCAAATGGACA+GGG	0.645295	1.2:+76486134	MS.gene34393:CDS
TCCCTATAGAAATCTACACA+AGG	0.653374	1.2:-76486370	None:intergenic
CACCTGAGAGAATCAATGGA+AGG	0.653823	1.2:+76486078	MS.gene34393:CDS
CCCTATAGAAATCTACACAA+GGG	0.666479	1.2:-76486369	None:intergenic
TATAGAAATCTACACAAGGG+AGG	0.702658	1.2:-76486366	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTTAGATAAACTAAATTTA+AGG	+	chr1.2:76485695-76485714	MS.gene34393:intron	10.0%
!!!	TTTATGTATAGTATTGATTT+TGG	+	chr1.2:76485961-76485980	MS.gene34393:intron	15.0%
!!	ACATAATATGTTTATGATTC+AGG	+	chr1.2:76485787-76485806	MS.gene34393:intron	20.0%
!!	GAATCATAAACATATTATGT+TGG	-	chr1.2:76485787-76485806	None:intergenic	20.0%
!	ATCGTCATTATTCAATGAAA+GGG	-	chr1.2:76486002-76486021	None:intergenic	25.0%
!	TATCGTCATTATTCAATGAA+AGG	-	chr1.2:76486003-76486022	None:intergenic	25.0%
!	TTGTTTGATCAAATATATGG+TGG	+	chr1.2:76486158-76486177	MS.gene34393:CDS	25.0%
!!	GGTTTGTTTGATCAAATATA+TGG	+	chr1.2:76486155-76486174	MS.gene34393:CDS	25.0%
	AATGGAAGAATTGCTATGTT+GGG	+	chr1.2:76486305-76486324	MS.gene34393:CDS	30.0%
	TTTGATCAAATATATGGTGG+TGG	+	chr1.2:76486161-76486180	MS.gene34393:CDS	30.0%
!!	TCTTTGATTCCATTTTTCCA+AGG	+	chr1.2:76486227-76486246	MS.gene34393:CDS	30.0%
	ACACTAACACCTTGGAAAAA+TGG	-	chr1.2:76486239-76486258	None:intergenic	35.0%
	ACCCTTAACATACTCTGTAA+AGG	-	chr1.2:76486345-76486364	None:intergenic	35.0%
	AGAACAGTTATGCTTACCTT+TGG	-	chr1.2:76485917-76485936	None:intergenic	35.0%
	GAATGGAAGAATTGCTATGT+TGG	+	chr1.2:76486304-76486323	MS.gene34393:CDS	35.0%
	GCCTTTACAGAGTATGTTAA+GGG	+	chr1.2:76486341-76486360	MS.gene34393:CDS	35.0%
	TATCTTGAAAGTTCGATCCA+TGG	+	chr1.2:76485657-76485676	MS.gene34393:CDS	35.0%
	TATGTTGGGTTTGATTGCTT+TGG	+	chr1.2:76486319-76486338	MS.gene34393:CDS	35.0%
	TGAGCAAAAAGAGCAATCAA+AGG	+	chr1.2:76485811-76485830	MS.gene34393:CDS	35.0%
!	AATGGAAGGTTAGCTATGAT+TGG	+	chr1.2:76486092-76486111	MS.gene34393:CDS	35.0%
!	GGTGATGATGATTGTGTAAT+CGG	-	chr1.2:76485766-76485785	None:intergenic	35.0%
!!	GTGATGATGATGGTGTAATT+GGG	-	chr1.2:76485847-76485866	None:intergenic	35.0%
!!!	ATTGGTTTTGTAGCAGCAAT+GGG	+	chr1.2:76486110-76486129	MS.gene34393:CDS	35.0%
	AACCTTAATTACCTCAGCCA+TGG	-	chr1.2:76485677-76485696	None:intergenic	40.0%
	ATCCATGGCTGAGGTAATTA+AGG	+	chr1.2:76485672-76485691	MS.gene34393:intron	40.0%
	GGCCTTTACAGAGTATGTTA+AGG	+	chr1.2:76486340-76486359	MS.gene34393:CDS	40.0%
!	ACAGATGAGCACCAAGTTTT+CGG	+	chr1.2:76486031-76486050	MS.gene34393:intron	40.0%
!	AGTGTTGAGTCCAAGTCAAA+AGG	+	chr1.2:76486254-76486273	MS.gene34393:CDS	40.0%
!!	AACCTTCCATTGATTCTCTC+AGG	-	chr1.2:76486083-76486102	None:intergenic	40.0%
!!	ATAGCATTCCTTCTGAAGCT+AGG	-	chr1.2:76485640-76485659	None:intergenic	40.0%
!!	GGTGATGATGATGGTGTAAT+TGG	-	chr1.2:76485848-76485867	None:intergenic	40.0%
!!	TTTTCGGACTTGATGGCATT+TGG	+	chr1.2:76486047-76486066	MS.gene34393:CDS	40.0%
!!!	GATTGGTTTTGTAGCAGCAA+TGG	+	chr1.2:76486109-76486128	MS.gene34393:CDS	40.0%
!!!	TTGGTTTTGTAGCAGCAATG+GGG	+	chr1.2:76486111-76486130	MS.gene34393:CDS	40.0%
	AAGCTAGGCATGTACACAGA+AGG	-	chr1.2:76485625-76485644	None:intergenic	45.0%
	AGCTAGGCATGTACACAGAA+GGG	-	chr1.2:76485624-76485643	None:intergenic	45.0%
	AGCTCAATGAGCACCATTTC+TGG	+	chr1.2:76485568-76485587	MS.gene34393:CDS	45.0%
	CACCTGAGAGAATCAATGGA+AGG	+	chr1.2:76486078-76486097	MS.gene34393:CDS	45.0%
	CAGAAGGGATGTTGCTGAAT+TGG	-	chr1.2:76485609-76485628	None:intergenic	45.0%
	GACTCTAGACTTGCCAGAAA+TGG	-	chr1.2:76485584-76485603	None:intergenic	45.0%
	GATGGTGTAATTGGGTCTAC+AGG	-	chr1.2:76485839-76485858	None:intergenic	45.0%
	GTGGAGATAGCAAATGGACA+GGG	+	chr1.2:76486134-76486153	MS.gene34393:CDS	45.0%
	TGCCATCAAGTCCGAAAACT+TGG	-	chr1.2:76486045-76486064	None:intergenic	45.0%
	TGGACTCAACACTAACACCT+TGG	-	chr1.2:76486247-76486266	None:intergenic	45.0%
	TGTACATGCCTAGCTTCAGA+AGG	+	chr1.2:76485629-76485648	MS.gene34393:CDS	45.0%
!	CACCAAGTTTTCGGACTTGA+TGG	+	chr1.2:76486040-76486059	MS.gene34393:CDS	45.0%
!!	GGACATGACACCTTTTGACT+TGG	-	chr1.2:76486267-76486286	None:intergenic	45.0%
!!	GTTGGTGTTGGTGATGATGA+TGG	-	chr1.2:76485857-76485876	None:intergenic	45.0%
!!!	TGGTTTTGTAGCAGCAATGG+GGG	+	chr1.2:76486112-76486131	MS.gene34393:CDS	45.0%
	ACACTTGTCCCCAAGAACCA+AGG	-	chr1.2:76486191-76486210	None:intergenic	50.0%
	ATGGGGGTGGAGATAGCAAA+TGG	+	chr1.2:76486128-76486147	MS.gene34393:CDS	50.0%
	ATTCCACAGCTCAGCATCTG+AGG	-	chr1.2:76486288-76486307	None:intergenic	50.0%
	CACTTGTCCCCAAGAACCAA+GGG	-	chr1.2:76486190-76486209	None:intergenic	50.0%
	CCAGCACCTGAGAGAATCAA+TGG	+	chr1.2:76486074-76486093	MS.gene34393:CDS	50.0%
	GAAAGTTCGATCCATGGCTG+AGG	+	chr1.2:76485663-76485682	MS.gene34393:CDS	50.0%
	GGTGGAGATAGCAAATGGAC+AGG	+	chr1.2:76486133-76486152	MS.gene34393:CDS	50.0%
	TCAGATGCTGAGCTGTGGAA+TGG	+	chr1.2:76486287-76486306	MS.gene34393:CDS	50.0%
!	CGAGTATAGGCTGGTTGTGA+TGG	-	chr1.2:76485890-76485909	None:intergenic	50.0%
!	CGGACTTGATGGCATTTGGT+GGG	+	chr1.2:76486051-76486070	MS.gene34393:CDS	50.0%
!	GTATAGGCTGGTTGTGATGG+TGG	-	chr1.2:76485887-76485906	None:intergenic	50.0%
!	TACCTTTGGTGCGCGAGTAT+AGG	-	chr1.2:76485903-76485922	None:intergenic	50.0%
!	TCGGACTTGATGGCATTTGG+TGG	+	chr1.2:76486050-76486069	MS.gene34393:CDS	50.0%
!	TTTTGTAGCAGCAATGGGGG+TGG	+	chr1.2:76486115-76486134	MS.gene34393:CDS	50.0%
!!	CCATTGATTCTCTCAGGTGC+TGG	-	chr1.2:76486077-76486096	None:intergenic	50.0%
!!	TATATGGTGGTGGCATCCCT+TGG	+	chr1.2:76486171-76486190	MS.gene34393:CDS	50.0%
	AGCCTATACTCGCGCACCAA+AGG	+	chr1.2:76485898-76485917	MS.gene34393:CDS	55.0%
	TGTCCTCAGATGCTGAGCTG+TGG	+	chr1.2:76486282-76486301	MS.gene34393:CDS	55.0%
!	GGTGGCATCCCTTGGTTCTT+GGG	+	chr1.2:76486179-76486198	MS.gene34393:CDS	55.0%
!	GTGGCATCCCTTGGTTCTTG+GGG	+	chr1.2:76486180-76486199	MS.gene34393:CDS	55.0%
!	TGGTGGCATCCCTTGGTTCT+TGG	+	chr1.2:76486178-76486197	MS.gene34393:CDS	55.0%
!	TTTGGTGCGCGAGTATAGGC+TGG	-	chr1.2:76485899-76485918	None:intergenic	55.0%
!!	TAGGCTGGTTGTGATGGTGG+TGG	-	chr1.2:76485884-76485903	None:intergenic	55.0%
!!	TGTGATGGTGGTGGTGGTGT+TGG	-	chr1.2:76485875-76485894	None:intergenic	55.0%
!!	GCTGGTTGTGATGGTGGTGG+TGG	-	chr1.2:76485881-76485900	None:intergenic	60.0%
!!	GGTGGTGGTGGTGTTGGTGT+TGG	-	chr1.2:76485869-76485888	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.2	gene	76485535	76486379	76485535	ID=MS.gene34393
chr1.2	mRNA	76485535	76486379	76485535	ID=MS.gene34393.t1;Parent=MS.gene34393
chr1.2	exon	76485535	76485684	76485535	ID=MS.gene34393.t1.exon1;Parent=MS.gene34393.t1
chr1.2	CDS	76485535	76485684	76485535	ID=cds.MS.gene34393.t1;Parent=MS.gene34393.t1
chr1.2	exon	76485809	76485919	76485809	ID=MS.gene34393.t1.exon2;Parent=MS.gene34393.t1
chr1.2	CDS	76485809	76485919	76485809	ID=cds.MS.gene34393.t1;Parent=MS.gene34393.t1
chr1.2	exon	76486035	76486379	76486035	ID=MS.gene34393.t1.exon3;Parent=MS.gene34393.t1
chr1.2	CDS	76486035	76486379	76486035	ID=cds.MS.gene34393.t1;Parent=MS.gene34393.t1

Gene Sequence

>MS.gene34393

ATGGCTGTATCATCTTATCAATCTATCATGTCAAGCTCAATGAGCACCATTTCTGGCAAGTCTAGAGTCAACCAATTCAGCAACATCCCTTCTGTGTACATGCCTAGCTTCAGAAGGAATGCTATCTTGAAAGTTCGATCCATGGCTGAGGTAATTAAGGTTTTAGATAAACTAAATTTAAGGTTATATACATATATAGTATTTGTTCATGCATAGTTGATTAAGTCACCGATTACACAATCATCATCACCAACATAATATGTTTATGATTCAGGATGAGCAAAAAGAGCAATCAAAGGTGCCTGTAGACCCAATTACACCATCATCATCACCAACACCAACACCACCACCACCATCACAACCAGCCTATACTCGCGCACCAAAGGTAAGCATAACTGTTCTTTCAAAGTTGTTTAGAAGAAATTATTTATGTATAGTATTGATTTTGGTAAAATTTTAAGAGACCCTTTCATTGAATAATGACGATAATAATATGACAGATGAGCACCAAGTTTTCGGACTTGATGGCATTTGGTGGGCCAGCACCTGAGAGAATCAATGGAAGGTTAGCTATGATTGGTTTTGTAGCAGCAATGGGGGTGGAGATAGCAAATGGACAGGGTTTGTTTGATCAAATATATGGTGGTGGCATCCCTTGGTTCTTGGGGACAAGTGTGTTGCTGACACTTGCTTCTTTGATTCCATTTTTCCAAGGTGTTAGTGTTGAGTCCAAGTCAAAAGGTGTCATGTCCTCAGATGCTGAGCTGTGGAATGGAAGAATTGCTATGTTGGGTTTGATTGCTTTGGCCTTTACAGAGTATGTTAAGGGTACCTCCCTTGTGTAG

Protein sequence

>MS.gene34393.t1

MAVSSYQSIMSSSMSTISGKSRVNQFSNIPSVYMPSFRRNAILKVRSMAEDEQKEQSKVPVDPITPSSSPTPTPPPPSQPAYTRAPKMSTKFSDLMAFGGPAPERINGRLAMIGFVAAMGVEIANGQGLFDQIYGGGIPWFLGTSVLLTLASLIPFFQGVSVESKSKGVMSSDAELWNGRIAMLGLIALAFTEYVKGTSLV