Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34803.t1 | XP_028184872.1 | 66.1 | 168 | 50 | 3 | 2 | 162 | 51 | 218 | 1.60E-52 | 215.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34803.t1 | Q9M2N5 | 37.5 | 144 | 80 | 3 | 3 | 146 | 562 | 695 | 1.1e-20 | 101.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34803.t1 | A0A392MM94 | 77.1 | 131 | 28 | 1 | 2 | 130 | 263 | 393 | 1.2e-52 | 215.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049254 | MS.gene34803 | 0.807495 | 4.92E-50 | -1.69E-46 |
| MS.gene050026 | MS.gene34803 | 0.809596 | 1.75E-50 | -1.69E-46 |
| MS.gene051279 | MS.gene34803 | 0.822184 | 2.71E-53 | -1.69E-46 |
| MS.gene051332 | MS.gene34803 | 0.806217 | 9.16E-50 | -1.69E-46 |
| MS.gene052519 | MS.gene34803 | 0.824548 | 7.60E-54 | -1.69E-46 |
| MS.gene052582 | MS.gene34803 | 0.831783 | 1.37E-55 | -1.69E-46 |
| MS.gene052584 | MS.gene34803 | 0.821114 | 4.80E-53 | -1.69E-46 |
| MS.gene05365 | MS.gene34803 | 0.812473 | 4.16E-51 | -1.69E-46 |
| MS.gene053678 | MS.gene34803 | 0.803284 | 3.75E-49 | -1.69E-46 |
| MS.gene05423 | MS.gene34803 | 0.81183 | 5.75E-51 | -1.69E-46 |
| MS.gene054651 | MS.gene34803 | 0.836633 | 8.34E-57 | -1.69E-46 |
| MS.gene054991 | MS.gene34803 | 0.810041 | 1.40E-50 | -1.69E-46 |
| MS.gene055228 | MS.gene34803 | 0.812413 | 4.29E-51 | -1.69E-46 |
| MS.gene056366 | MS.gene34803 | 0.804774 | 1.84E-49 | -1.69E-46 |
| MS.gene05686 | MS.gene34803 | 0.810831 | 9.48E-51 | -1.69E-46 |
| MS.gene05690 | MS.gene34803 | 0.821048 | 4.97E-53 | -1.69E-46 |
| MS.gene057102 | MS.gene34803 | 0.80144 | 9.00E-49 | -1.69E-46 |
| MS.gene058037 | MS.gene34803 | 0.801886 | 7.29E-49 | -1.69E-46 |
| MS.gene058625 | MS.gene34803 | 0.813539 | 2.43E-51 | -1.69E-46 |
| MS.gene059625 | MS.gene34803 | 0.832081 | 1.16E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34803.t1 | AT3G42170 | 39.669 | 121 | 65 | 2 | 3 | 123 | 540 | 652 | 3.12e-24 | 98.6 |
| MS.gene34803.t1 | AT3G42170 | 39.669 | 121 | 65 | 2 | 3 | 123 | 562 | 674 | 3.17e-24 | 98.6 |
| MS.gene34803.t1 | AT1G18560 | 36.000 | 100 | 58 | 3 | 27 | 123 | 553 | 649 | 3.58e-13 | 66.6 |
Find 28 sgRNAs with CRISPR-Local
Find 33 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCAGCTTCACTCTTCAAAA+TGG | 0.170935 | 8.3:-40106183 | None:intergenic |
| AACGGAGTTGGATCATTATC+TGG | 0.259746 | 8.3:+40105623 | MS.gene34803:CDS |
| CCACATCAGAATATCAAAAT+TGG | 0.289304 | 8.3:-40105670 | None:intergenic |
| TCAGAAGCTACTGAAGTAAT+AGG | 0.323940 | 8.3:-40105756 | None:intergenic |
| ATATCAAAATTGGCACTTCT+AGG | 0.340733 | 8.3:-40105660 | None:intergenic |
| TTTGCAATTTCTTGCAATGT+TGG | 0.382104 | 8.3:-40105717 | None:intergenic |
| AAGATCTTACATTCATCATC+AGG | 0.417940 | 8.3:-40106054 | None:intergenic |
| TCAGCTTCACTCTTCAAAAT+GGG | 0.432393 | 8.3:-40106182 | None:intergenic |
| GCTAATTCTGTGGCTAATGA+TGG | 0.459373 | 8.3:+40105540 | MS.gene34803:CDS |
| CTCATCGTAGTCGACTTCAT+TGG | 0.485204 | 8.3:+40105814 | MS.gene34803:CDS |
| ATATGGAGCAAGGAGAATAA+TGG | 0.491041 | 8.3:+40105873 | MS.gene34803:CDS |
| TAGAACATCATTATATAAAA+CGG | 0.495038 | 8.3:+40105605 | MS.gene34803:CDS |
| ATCATTATATAAAACGGAGT+TGG | 0.499119 | 8.3:+40105611 | MS.gene34803:CDS |
| CTTTGATGTGTGCTAGAAGT+TGG | 0.508597 | 8.3:+40105850 | MS.gene34803:CDS |
| ACTTCATTGGACAACCTTGG+AGG | 0.521822 | 8.3:+40105827 | MS.gene34803:CDS |
| GAATCAGCATTCAGTACAAG+TGG | 0.537506 | 8.3:+40105777 | MS.gene34803:CDS |
| TCGACTTCATTGGACAACCT+TGG | 0.544964 | 8.3:+40105824 | MS.gene34803:CDS |
| TGTGTGCTAGAAGTTGGATA+TGG | 0.560174 | 8.3:+40105856 | MS.gene34803:CDS |
| ACGGAGTTGGATCATTATCT+GGG | 0.571432 | 8.3:+40105624 | MS.gene34803:CDS |
| CTGATGTGGTGGAAGTTGAA+TGG | 0.578791 | 8.3:+40105684 | MS.gene34803:CDS |
| ATTATGATGCATATGTCAAG+AGG | 0.590157 | 8.3:+40105571 | MS.gene34803:CDS |
| AAGTTCAATCAAGACACTTG+TGG | 0.595420 | 8.3:+40105504 | None:intergenic |
| AGACACTTGTGGTGAATCGT+CGG | 0.609844 | 8.3:+40105515 | MS.gene34803:CDS |
| AGCACACATCAAAGCCTCCA+AGG | 0.630649 | 8.3:-40105841 | None:intergenic |
| TAGAAGTTGGATATGGAGCA+AGG | 0.637735 | 8.3:+40105863 | MS.gene34803:CDS |
| ATAGGTGAAATATTGACTAG+TGG | 0.646439 | 8.3:+40106151 | MS.gene34803:intron |
| ATCGTCGGAAGCTAATTCTG+TGG | 0.696219 | 8.3:+40105530 | MS.gene34803:CDS |
| CAATGAAGTCGACTACGATG+AGG | 0.702001 | 8.3:-40105813 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ACTTGTATTTTTTATTATAT+AGG | + | chr8.3:40106133-40106152 | MS.gene34803:intron | 10.0% |
| !! | AAACATTAAAGTTAGTAAAT+TGG | - | chr8.3:40106105-40106124 | None:intergenic | 15.0% |
| !! | TAGAACATCATTATATAAAA+CGG | + | chr8.3:40105605-40105624 | MS.gene34803:CDS | 15.0% |
| !! | ATTAAAGTTAGTAAATTGGA+AGG | - | chr8.3:40106101-40106120 | None:intergenic | 20.0% |
| !! | CTTTCAATTTACATATCTTA+AGG | + | chr8.3:40105913-40105932 | MS.gene34803:intron | 20.0% |
| ! | ATCATTATATAAAACGGAGT+TGG | + | chr8.3:40105611-40105630 | MS.gene34803:CDS | 25.0% |
| ! | GGAAGGTAGTATTAAATAAA+TGG | - | chr8.3:40106084-40106103 | None:intergenic | 25.0% |
| AAGATCTTACATTCATCATC+AGG | - | chr8.3:40106057-40106076 | None:intergenic | 30.0% | |
| ACTGCATTTGCATTATTTAC+AGG | + | chr8.3:40105976-40105995 | MS.gene34803:intron | 30.0% | |
| ATATCAAAATTGGCACTTCT+AGG | - | chr8.3:40105663-40105682 | None:intergenic | 30.0% | |
| ATTATGATGCATATGTCAAG+AGG | + | chr8.3:40105571-40105590 | MS.gene34803:CDS | 30.0% | |
| CCACATCAGAATATCAAAAT+TGG | - | chr8.3:40105673-40105692 | None:intergenic | 30.0% | |
| TTTGCAATTTCTTGCAATGT+TGG | - | chr8.3:40105720-40105739 | None:intergenic | 30.0% | |
| ! | ATAGGTGAAATATTGACTAG+TGG | + | chr8.3:40106151-40106170 | MS.gene34803:intron | 30.0% |
| !! | ATTTTGATATTCTGATGTGG+TGG | + | chr8.3:40105673-40105692 | MS.gene34803:CDS | 30.0% |
| !! | CCAATTTTGATATTCTGATG+TGG | + | chr8.3:40105670-40105689 | MS.gene34803:CDS | 30.0% |
| ATATGGAGCAAGGAGAATAA+TGG | + | chr8.3:40105873-40105892 | MS.gene34803:CDS | 35.0% | |
| TCAGAAGCTACTGAAGTAAT+AGG | - | chr8.3:40105759-40105778 | None:intergenic | 35.0% | |
| AACGGAGTTGGATCATTATC+TGG | + | chr8.3:40105623-40105642 | MS.gene34803:CDS | 40.0% | |
| ACGGAGTTGGATCATTATCT+GGG | + | chr8.3:40105624-40105643 | MS.gene34803:CDS | 40.0% | |
| CTTTGATGTGTGCTAGAAGT+TGG | + | chr8.3:40105850-40105869 | MS.gene34803:CDS | 40.0% | |
| GAATCAGCATTCAGTACAAG+TGG | + | chr8.3:40105777-40105796 | MS.gene34803:CDS | 40.0% | |
| GCTAATTCTGTGGCTAATGA+TGG | + | chr8.3:40105540-40105559 | MS.gene34803:CDS | 40.0% | |
| TAGAAGTTGGATATGGAGCA+AGG | + | chr8.3:40105863-40105882 | MS.gene34803:CDS | 40.0% | |
| TGTGTGCTAGAAGTTGGATA+TGG | + | chr8.3:40105856-40105875 | MS.gene34803:CDS | 40.0% | |
| ACTTCATTGGACAACCTTGG+AGG | + | chr8.3:40105827-40105846 | MS.gene34803:CDS | 45.0% | |
| AGACACTTGTGGTGAATCGT+CGG | + | chr8.3:40105515-40105534 | MS.gene34803:CDS | 45.0% | |
| ATCGTCGGAAGCTAATTCTG+TGG | + | chr8.3:40105530-40105549 | MS.gene34803:CDS | 45.0% | |
| CAATGAAGTCGACTACGATG+AGG | - | chr8.3:40105816-40105835 | None:intergenic | 45.0% | |
| TCGACTTCATTGGACAACCT+TGG | + | chr8.3:40105824-40105843 | MS.gene34803:CDS | 45.0% | |
| ! | CTCATCGTAGTCGACTTCAT+TGG | + | chr8.3:40105814-40105833 | MS.gene34803:CDS | 45.0% |
| ! | CTGATGTGGTGGAAGTTGAA+TGG | + | chr8.3:40105684-40105703 | MS.gene34803:CDS | 45.0% |
| AGCACACATCAAAGCCTCCA+AGG | - | chr8.3:40105844-40105863 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 40105507 | 40106198 | 40105507 | ID=MS.gene34803 |
| chr8.3 | mRNA | 40105507 | 40106198 | 40105507 | ID=MS.gene34803.t1;Parent=MS.gene34803 |
| chr8.3 | exon | 40105507 | 40105894 | 40105507 | ID=MS.gene34803.t1.exon1;Parent=MS.gene34803.t1 |
| chr8.3 | CDS | 40105507 | 40105894 | 40105507 | ID=cds.MS.gene34803.t1;Parent=MS.gene34803.t1 |
| chr8.3 | exon | 40105998 | 40106066 | 40105998 | ID=MS.gene34803.t1.exon2;Parent=MS.gene34803.t1 |
| chr8.3 | CDS | 40105998 | 40106066 | 40105998 | ID=cds.MS.gene34803.t1;Parent=MS.gene34803.t1 |
| chr8.3 | exon | 40106155 | 40106198 | 40106155 | ID=MS.gene34803.t1.exon3;Parent=MS.gene34803.t1 |
| chr8.3 | CDS | 40106155 | 40106198 | 40106155 | ID=cds.MS.gene34803.t1;Parent=MS.gene34803.t1 |
| Gene Sequence |
| Protein sequence |
