AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene37023


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene37023.t1 MTR_6g013410 96.875 64 2 0 1 64 38 101 3.51e-39 124
MS.gene37023.t1 MTR_6g013210 83.077 65 10 1 1 64 1 65 4.08e-31 103
MS.gene37023.t1 MTR_4g081180 76.923 65 14 1 1 64 150 214 4.73e-27 97.4
MS.gene37023.t1 MTR_8g089765 71.429 49 13 1 1 48 1 49 1.12e-17 70.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene37023.t1 AT5G03850 81.250 64 12 0 1 64 1 64 8.10e-32 105
MS.gene37023.t1 AT3G10090 81.250 64 12 0 1 64 1 64 8.10e-32 105
MS.gene37023.t1 AT5G64140 79.688 64 13 0 1 64 1 64 1.99e-31 103

Find 16 sgRNAs with CRISPR-Local

Find 16 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGTCATCTCCTTCTCTAAT+GGG 0.330219 8.3:-76672240 None:intergenic
AATGTCATCTCCTTCTCTAA+TGG 0.406581 8.3:-76672241 None:intergenic
CGCTCAAGTTGAGAAAGTTA+TGG 0.416411 8.3:+76672131 MS.gene37023:CDS
AACCTGAGTGACCTGTCCTC+TGG 0.457012 8.3:-76672166 None:intergenic
GCTCAAGTTGAGAAAGTTAT+GGG 0.472061 8.3:+76672132 MS.gene37023:CDS
GAGAAAGTTATGGGTCGTAC+TGG 0.491245 8.3:+76672141 MS.gene37023:CDS
CTTCTCTCTCAGACTCAAGT+AGG 0.493446 8.3:-76672266 None:intergenic
TGTCATCTCCTTCTCTAATG+GGG 0.597877 8.3:-76672239 None:intergenic
TCGTACTGGATCCAGAGGAC+AGG 0.601747 8.3:+76672155 MS.gene37023:CDS
ATCCAGAGGACAGGTCACTC+AGG 0.602839 8.3:+76672164 MS.gene37023:CDS
ATGAGAATCGCTACATCATG+AGG 0.604858 8.3:+76672205 MS.gene37023:CDS
GTGAAGGGCCCCATTAGAGA+AGG 0.605680 8.3:+76672231 MS.gene37023:CDS
TTCTCTCTCAGACTCAAGTA+GGG 0.606054 8.3:-76672265 None:intergenic
CTACATCATGAGGAATGTGA+AGG 0.633338 8.3:+76672215 MS.gene37023:CDS
TACATCATGAGGAATGTGAA+GGG 0.640443 8.3:+76672216 MS.gene37023:CDS
ATGGGTCGTACTGGATCCAG+AGG 0.663896 8.3:+76672150 MS.gene37023:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AATGTCATCTCCTTCTCTAA+TGG - chr8.3:76672244-76672263 None:intergenic 35.0%
ATGTCATCTCCTTCTCTAAT+GGG - chr8.3:76672243-76672262 None:intergenic 35.0%
TACATCATGAGGAATGTGAA+GGG + chr8.3:76672216-76672235 MS.gene37023:CDS 35.0%
!! GCTCAAGTTGAGAAAGTTAT+GGG + chr8.3:76672132-76672151 MS.gene37023:CDS 35.0%
ATGAGAATCGCTACATCATG+AGG + chr8.3:76672205-76672224 MS.gene37023:CDS 40.0%
CTACATCATGAGGAATGTGA+AGG + chr8.3:76672215-76672234 MS.gene37023:CDS 40.0%
TGTCATCTCCTTCTCTAATG+GGG - chr8.3:76672242-76672261 None:intergenic 40.0%
TTCTCTCTCAGACTCAAGTA+GGG - chr8.3:76672268-76672287 None:intergenic 40.0%
!! CGCTCAAGTTGAGAAAGTTA+TGG + chr8.3:76672131-76672150 MS.gene37023:CDS 40.0%
CTTCTCTCTCAGACTCAAGT+AGG - chr8.3:76672269-76672288 None:intergenic 45.0%
GAGAAAGTTATGGGTCGTAC+TGG + chr8.3:76672141-76672160 MS.gene37023:CDS 45.0%
AACCTGAGTGACCTGTCCTC+TGG - chr8.3:76672169-76672188 None:intergenic 55.0%
ATCCAGAGGACAGGTCACTC+AGG + chr8.3:76672164-76672183 MS.gene37023:CDS 55.0%
ATGGGTCGTACTGGATCCAG+AGG + chr8.3:76672150-76672169 MS.gene37023:CDS 55.0%
GTGAAGGGCCCCATTAGAGA+AGG + chr8.3:76672231-76672250 MS.gene37023:CDS 55.0%
TCGTACTGGATCCAGAGGAC+AGG + chr8.3:76672155-76672174 MS.gene37023:CDS 55.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 76672111 76672305 76672111 ID=MS.gene37023
chr8.3 mRNA 76672111 76672305 76672111 ID=MS.gene37023.t1;Parent=MS.gene37023
chr8.3 exon 76672111 76672305 76672111 ID=MS.gene37023.t1.exon1;Parent=MS.gene37023.t1
chr8.3 CDS 76672111 76672305 76672111 ID=cds.MS.gene37023.t1;Parent=MS.gene37023.t1
Gene Sequence

>MS.gene37023

ATGGAATCACAGATTAAGCACGCTCAAGTTGAGAAAGTTATGGGTCGTACTGGATCCAGAGGACAGGTCACTCAGGTTAGAGTCAAGATTCTGCATGAGAATCGCTACATCATGAGGAATGTGAAGGGCCCCATTAGAGAAGGAGATGACATTGCCCTACTTGAGTCTGAGAGAGAAGCAAGAAGACTCCGATAG

Protein sequence

>MS.gene37023.t1

MESQIKHAQVEKVMGRTGSRGQVTQVRVKILHENRYIMRNVKGPIREGDDIALLESEREARRLR