AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene37574


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene37574.t1 MTR_5g088980 70.950 179 52 0 4 182 76 254 4.78e-84 254
MS.gene37574.t1 MTR_7g089080 50.279 179 85 3 5 182 113 288 7.75e-49 169
MS.gene37574.t1 MTR_5g023790 46.903 113 59 1 71 182 150 262 3.05e-30 113
MS.gene37574.t1 MTR_5g023790 46.903 113 59 1 71 182 150 262 5.30e-30 113
MS.gene37574.t1 MTR_5g023790 46.903 113 59 1 71 182 150 262 7.22e-30 113
MS.gene37574.t1 MTR_8g074000 44.545 110 60 1 74 182 157 266 1.31e-27 107
MS.gene37574.t1 MTR_1g011470 40.945 127 68 4 58 182 131 252 9.68e-22 91.3
MS.gene37574.t1 MTR_3g088415 39.130 138 72 4 56 182 195 331 2.62e-21 90.1
MS.gene37574.t1 MTR_3g088415 39.130 138 72 4 56 182 195 331 4.31e-21 90.1
MS.gene37574.t1 MTR_3g088415 39.130 138 72 4 56 182 195 331 4.71e-21 89.7
MS.gene37574.t1 MTR_3g107970 42.593 108 59 3 77 182 198 304 9.24e-21 89.0
MS.gene37574.t1 MTR_2g102520 40.458 131 68 4 61 182 177 306 1.34e-19 85.5
MS.gene37574.t1 MTR_3g102520 40.566 106 59 2 81 182 79 184 3.19e-18 81.3
MS.gene37574.t1 MTR_3g102530 40.566 106 59 1 81 182 72 177 1.01e-17 79.7
MS.gene37574.t1 MTR_2g102550 37.037 135 79 4 53 182 125 258 2.99e-17 79.0
MS.gene37574.t1 MTR_1g018680 41.748 103 55 3 84 182 83 184 3.26e-17 78.2
MS.gene37574.t1 MTR_3g102510 44.565 92 49 2 93 182 74 165 6.60e-17 77.4
MS.gene37574.t1 MTR_3g060770 37.719 114 63 3 76 184 13 123 7.57e-17 76.6
MS.gene37574.t1 MTR_3g088405 37.963 108 64 3 77 182 228 334 1.15e-16 77.4
MS.gene37574.t1 MTR_3g061680 40.196 102 55 2 86 184 15 113 2.37e-16 75.1
MS.gene37574.t1 MTR_3g060400 40.206 97 52 2 91 184 26 119 1.47e-15 73.2
MS.gene37574.t1 MTR_3g460990 40.206 97 52 2 91 184 26 119 1.47e-15 73.2
MS.gene37574.t1 MTR_3g060820 38.614 101 56 2 87 184 16 113 6.09e-15 71.2
MS.gene37574.t1 MTR_5g011900 37.273 110 68 1 75 183 52 161 1.37e-14 69.7
MS.gene37574.t1 MTR_5g088940 55.738 61 8 1 114 174 1 42 3.94e-14 64.3
MS.gene37574.t1 MTR_6g045270 38.542 96 53 2 92 184 45 137 1.18e-12 65.1
MS.gene37574.t1 MTR_2g005960 32.000 125 70 4 73 182 102 226 1.48e-12 63.9
MS.gene37574.t1 MTR_3g102560 47.222 72 37 1 112 182 86 157 5.78e-12 63.2
MS.gene37574.t1 MTR_3g060410 44.118 68 35 1 120 184 131 198 9.81e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene37574.t1 AT2G26760 49.704 169 75 3 14 182 61 219 3.56e-47 158
MS.gene37574.t1 AT4G37490 51.429 175 73 3 12 182 88 254 1.10e-46 158
MS.gene37574.t1 AT5G06150 47.222 180 91 2 5 182 94 271 3.78e-45 152
MS.gene37574.t1 AT5G06150 47.222 180 91 2 5 182 94 271 3.66e-44 152
MS.gene37574.t1 AT3G11520 47.368 171 68 4 14 182 98 248 4.16e-39 137
MS.gene37574.t1 AT3G11520 47.059 170 70 4 14 182 98 248 5.16e-39 137
MS.gene37574.t1 AT1G76310 48.673 113 56 2 72 182 156 268 4.02e-31 115
MS.gene37574.t1 AT1G34460 43.284 134 73 1 36 169 215 345 5.82e-31 117
MS.gene37574.t1 AT1G34460 43.284 134 73 1 36 169 215 345 5.82e-31 117
MS.gene37574.t1 AT1G34460 42.537 134 77 0 36 169 201 334 5.86e-31 117
MS.gene37574.t1 AT1G34460 42.537 134 77 0 36 169 215 348 6.15e-31 117
MS.gene37574.t1 AT1G34460 42.537 134 77 0 36 169 215 348 6.45e-31 117
MS.gene37574.t1 AT1G34460 42.537 134 77 0 36 169 215 348 6.48e-31 117
MS.gene37574.t1 AT1G34460 42.537 134 77 0 36 169 215 348 6.48e-31 117
MS.gene37574.t1 AT1G34460 42.537 134 77 0 36 169 215 348 6.48e-31 117
MS.gene37574.t1 AT1G34460 42.537 134 77 0 36 169 215 348 6.48e-31 117
MS.gene37574.t1 AT1G76310 48.673 113 56 2 72 182 156 268 1.28e-30 115
MS.gene37574.t1 AT1G76310 48.673 113 56 2 72 182 156 268 1.70e-30 115
MS.gene37574.t1 AT1G76310 48.673 113 56 2 72 182 156 268 2.35e-30 115
MS.gene37574.t1 AT1G20610 44.144 111 61 1 73 182 157 267 3.81e-29 111
MS.gene37574.t1 AT4G35620 50.926 108 52 1 76 182 155 262 7.74e-23 94.0
MS.gene37574.t1 AT4G35620 50.926 108 52 1 76 182 155 262 9.99e-23 94.4
MS.gene37574.t1 AT1G44110 43.119 109 59 3 76 182 178 285 1.86e-22 93.6
MS.gene37574.t1 AT1G16330 42.574 101 57 1 83 182 385 485 5.75e-22 92.8
MS.gene37574.t1 AT2G17620 47.368 114 59 1 70 182 148 261 1.05e-21 91.3
MS.gene37574.t1 AT5G43080 38.158 152 90 4 34 182 28 178 1.56e-21 90.1
MS.gene37574.t1 AT1G77390 43.119 109 59 3 76 182 162 269 1.66e-21 90.9
MS.gene37574.t1 AT5G43080 38.158 152 90 4 34 182 28 178 1.90e-21 90.1
MS.gene37574.t1 AT1G47220 36.774 155 86 4 34 184 5 151 5.13e-21 88.6
MS.gene37574.t1 AT1G80370 43.220 118 64 3 67 182 172 288 8.49e-21 89.0
MS.gene37574.t1 AT1G47230 37.226 137 81 3 50 182 53 188 1.39e-20 87.8
MS.gene37574.t1 AT1G47210 34.320 169 93 5 20 182 39 195 1.76e-20 87.4
MS.gene37574.t1 AT5G11300 40.000 125 72 3 60 182 140 263 4.31e-20 87.0
MS.gene37574.t1 AT5G11300 40.000 125 72 3 60 182 140 263 4.67e-20 86.7
MS.gene37574.t1 AT1G47210 35.664 143 76 4 41 179 62 192 1.46e-19 82.0
MS.gene37574.t1 AT1G47230 36.957 138 81 4 50 182 53 189 5.89e-19 83.2
MS.gene37574.t1 AT5G25380 39.450 109 63 3 76 182 157 264 9.25e-18 80.1
MS.gene37574.t1 AT5G25380 39.450 109 63 3 76 182 163 270 9.55e-18 80.1
MS.gene37574.t1 AT5G25380 39.450 109 63 3 76 182 157 264 1.05e-17 80.1
MS.gene37574.t1 AT5G25380 39.450 109 63 3 76 182 157 264 1.05e-17 80.1
MS.gene37574.t1 AT1G15570 42.593 108 59 3 77 182 173 279 3.93e-17 78.6

Find 27 sgRNAs with CRISPR-Local

Find 42 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAGTCAGATAGAAAGTTTC+TGG 0.200703 5.4:+70070704 None:intergenic
CTCCTCTTAGCCGTTATATC+TGG 0.320074 5.4:+70070779 None:intergenic
TTAGCACCTTGTGATGCATT+TGG 0.341745 5.4:+70071446 None:intergenic
AGAGAGGAATTTCAACTTGT+TGG 0.397326 5.4:-70070641 MS.gene37574:CDS
AATTACTGAACTACAAGCTT+TGG 0.414807 5.4:+70071198 None:intergenic
TGCTGATAAGGATAATGAAT+TGG 0.420062 5.4:-70071005 MS.gene37574:CDS
AGTAATTTCAGCTAGAAGAA+AGG 0.442539 5.4:-70071182 MS.gene37574:intron
ACAAGCTTTGGCATTTCTCC+TGG 0.456328 5.4:+70071210 None:intergenic
ACAAGTTGAAATTCCTCTCT+AGG 0.464310 5.4:+70070644 None:intergenic
GAACATTGATACTGCTGATA+AGG 0.486849 5.4:-70071017 MS.gene37574:CDS
TGTCAAATTCAGGCTTCTTG+TGG 0.503060 5.4:-70071064 MS.gene37574:intron
GGCTTCACAGCCAGATATAA+CGG 0.509060 5.4:-70070789 MS.gene37574:CDS
GGAGATCAATTCTTGTTGAC+TGG 0.513087 5.4:-70070760 MS.gene37574:CDS
TGTACGAGTACATGATTACA+TGG 0.521386 5.4:-70070810 MS.gene37574:CDS
GAGCTGATGACAATAACTTC+AGG 0.522150 5.4:+70071301 None:intergenic
AGCTTTGGCATTTCTCCTGG+TGG 0.529215 5.4:+70071213 None:intergenic
ACAATAACTTCAGGTACTGT+AGG 0.532942 5.4:+70071310 None:intergenic
TGACTCTTAATATTGTTGAC+CGG 0.533961 5.4:-70070688 MS.gene37574:CDS
GATCAATTCTTGTTGACTGG+TGG 0.548572 5.4:-70070757 MS.gene37574:CDS
CAAGTTGAAATTCCTCTCTA+GGG 0.572482 5.4:+70070645 None:intergenic
TGAAGAATATGCGGAAGTGA+AGG 0.574870 5.4:-70071275 MS.gene37574:CDS
TTCTTGTGGAATTGTGCTAG+AGG 0.585996 5.4:-70071050 MS.gene37574:CDS
AGCCAGATATAACGGCTAAG+AGG 0.588033 5.4:-70070781 MS.gene37574:CDS
CACAAAAGCTGTCCCTAGAG+AGG 0.594294 5.4:-70070657 MS.gene37574:CDS
CAGCTCAGATGAAGAATATG+CGG 0.656465 5.4:-70071284 MS.gene37574:CDS
ATGTTTCCAAATGCATCACA+AGG 0.671375 5.4:-70071452 MS.gene37574:intron
ATTTAAGAGAAAGATCCACC+AGG 0.681917 5.4:-70071228 MS.gene37574:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAAAAAAATTAGACA+TGG + chr5.4:70070684-70070703 None:intergenic 10.0%
!! ATATGAGAAATAGAAAATAA+TGG + chr5.4:70070906-70070925 None:intergenic 15.0%
!!! TTTTTTTTTTGTTTTGTTGC+AGG - chr5.4:70070696-70070715 MS.gene37574:CDS 20.0%
! TAGTTTCTTTCTTTCTTTGT+TGG - chr5.4:70071182-70071201 MS.gene37574:intron 25.0%
! TTGAATTTCATGAATGACTT+AGG - chr5.4:70071219-70071238 MS.gene37574:CDS 25.0%
!! TCTATTTCTCATATGTGATA+TGG - chr5.4:70070912-70070931 MS.gene37574:intron 25.0%
AATTACTGAACTACAAGCTT+TGG + chr5.4:70070861-70070880 None:intergenic 30.0%
AGTAATTTCAGCTAGAAGAA+AGG - chr5.4:70070874-70070893 MS.gene37574:intron 30.0%
CATGGAAATAGATAAACATG+AGG + chr5.4:70070666-70070685 None:intergenic 30.0%
GAACAATGTTTGTCAAATTC+AGG - chr5.4:70070982-70071001 MS.gene37574:CDS 30.0%
TGACTCTTAATATTGTTGAC+CGG - chr5.4:70071368-70071387 MS.gene37574:intron 30.0%
TGCTGATAAGGATAATGAAT+TGG - chr5.4:70071051-70071070 MS.gene37574:CDS 30.0%
! GTGATATGGTTTTCGATTTA+GGG - chr5.4:70070926-70070945 MS.gene37574:intron 30.0%
! TGTGATATGGTTTTCGATTT+AGG - chr5.4:70070925-70070944 MS.gene37574:intron 30.0%
!! TAGGGTTCTATTTTTCAATG+TGG - chr5.4:70070944-70070963 MS.gene37574:intron 30.0%
AGAGAGGAATTTCAACTTGT+TGG - chr5.4:70071415-70071434 MS.gene37574:intron 35.0%
AGAGTCAGATAGAAAGTTTC+TGG + chr5.4:70071355-70071374 None:intergenic 35.0%
ATGTTTCCAAATGCATCACA+AGG - chr5.4:70070604-70070623 MS.gene37574:CDS 35.0%
ATTTAAGAGAAAGATCCACC+AGG - chr5.4:70070828-70070847 MS.gene37574:intron 35.0%
GAACATTGATACTGCTGATA+AGG - chr5.4:70071039-70071058 MS.gene37574:CDS 35.0%
TGTACGAGTACATGATTACA+TGG - chr5.4:70071246-70071265 MS.gene37574:CDS 35.0%
! ACAAGTTGAAATTCCTCTCT+AGG + chr5.4:70071415-70071434 None:intergenic 35.0%
! ACAATAACTTCAGGTACTGT+AGG + chr5.4:70070749-70070768 None:intergenic 35.0%
! CAAGTTGAAATTCCTCTCTA+GGG + chr5.4:70071414-70071433 None:intergenic 35.0%
CAGCTCAGATGAAGAATATG+CGG - chr5.4:70070772-70070791 MS.gene37574:CDS 40.0%
GAGCTGATGACAATAACTTC+AGG + chr5.4:70070758-70070777 None:intergenic 40.0%
GGAGATCAATTCTTGTTGAC+TGG - chr5.4:70071296-70071315 MS.gene37574:CDS 40.0%
TGAAGAATATGCGGAAGTGA+AGG - chr5.4:70070781-70070800 MS.gene37574:CDS 40.0%
TGTCAAATTCAGGCTTCTTG+TGG - chr5.4:70070992-70071011 MS.gene37574:CDS 40.0%
TTAGCACCTTGTGATGCATT+TGG + chr5.4:70070613-70070632 None:intergenic 40.0%
TTCTTGTGGAATTGTGCTAG+AGG - chr5.4:70071006-70071025 MS.gene37574:CDS 40.0%
! GATCAATTCTTGTTGACTGG+TGG - chr5.4:70071299-70071318 MS.gene37574:CDS 40.0%
! TTTTGTTGCAGGACTCACTT+AGG - chr5.4:70070707-70070726 MS.gene37574:CDS 40.0%
!! CGCTTTTTACAAACTCTCTG+AGG - chr5.4:70071102-70071121 MS.gene37574:intron 40.0%
AGCCAGATATAACGGCTAAG+AGG - chr5.4:70071275-70071294 MS.gene37574:CDS 45.0%
CTCCTCTTAGCCGTTATATC+TGG + chr5.4:70071280-70071299 None:intergenic 45.0%
GGCTTCACAGCCAGATATAA+CGG - chr5.4:70071267-70071286 MS.gene37574:CDS 45.0%
! ACAAGCTTTGGCATTTCTCC+TGG + chr5.4:70070849-70070868 None:intergenic 45.0%
! CAGCTTTTGTGGACAAGAAC+CGG + chr5.4:70071390-70071409 None:intergenic 45.0%
CACAAAAGCTGTCCCTAGAG+AGG - chr5.4:70071399-70071418 MS.gene37574:intron 50.0%
! AGCTTTGGCATTTCTCCTGG+TGG + chr5.4:70070846-70070865 None:intergenic 50.0%
!! CTCTCTAGGGACAGCTTTTG+TGG + chr5.4:70071401-70071420 None:intergenic 50.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 70070598 70071480 70070598 ID=MS.gene37574
chr5.4 mRNA 70070598 70071480 70070598 ID=MS.gene37574.t1;Parent=MS.gene37574
chr5.4 exon 70071453 70071480 70071453 ID=MS.gene37574.t1.exon1;Parent=MS.gene37574.t1
chr5.4 CDS 70071453 70071480 70071453 ID=cds.MS.gene37574.t1;Parent=MS.gene37574.t1
chr5.4 exon 70071183 70071349 70071183 ID=MS.gene37574.t1.exon2;Parent=MS.gene37574.t1
chr5.4 CDS 70071183 70071349 70071183 ID=cds.MS.gene37574.t1;Parent=MS.gene37574.t1
chr5.4 exon 70070955 70071074 70070955 ID=MS.gene37574.t1.exon3;Parent=MS.gene37574.t1
chr5.4 CDS 70070955 70071074 70070955 ID=cds.MS.gene37574.t1;Parent=MS.gene37574.t1
chr5.4 exon 70070598 70070837 70070598 ID=MS.gene37574.t1.exon4;Parent=MS.gene37574.t1
chr5.4 CDS 70070598 70070837 70070598 ID=cds.MS.gene37574.t1;Parent=MS.gene37574.t1
Gene Sequence

>MS.gene37574

ATGCTAATGTTTCCAAATGCATCACAAGGTGCTAATTTTTTAATTTTATTAAAATTAAATTATTACCTCATGTTTATCTATTTCCATGTCTAATTTTTTTTTTTTTTTGTTTTGTTGCAGGACTCACTTAGGTGCTATTGCTCAGAAGCCTACAGTACCTGAAGTTATTGTCATCAGCTCAGATGAAGAATATGCGGAAGTGAAGGAGAAACAAGTTGTTAAAAGAAGAAATTTAAGAGAAAGATCCACCAGGAGAAATGCCAAAGCTTGTAGTTCAGTAATTTCAGCTAGAAGAAAGGTTTTTTCCATTATTTTCTATTTCTCATATGTGATATGGTTTTCGATTTAGGGTTCTATTTTTCAATGTGGACTAATAATAAGTATGAACAATGTTTGTCAAATTCAGGCTTCTTGTGGAATTGTGCTAGAGGATTTAGTTGTGAACATTGATACTGCTGATAAGGATAATGAATTGGCTGTAACTGAGTACGTTGATGACATCTACGCTTTTTACAAACTCTCTGAGGTAATGTTTTATGCTTTGCTTTGATTTGTTTTGTGTTCTTTCTTTATATATATGTCTCTAGTTTCTTTCTTTCTTTGTTGGCATTCTCTAATGTTTTGAATTTCATGAATGACTTAGGTTGATGTACGAGTACATGATTACATGGCTTCACAGCCAGATATAACGGCTAAGAGGAGATCAATTCTTGTTGACTGGTGGAATGACGTGCATCGAACATTCAAACTCATGCCAGAAACTTTCTATCTGACTCTTAATATTGTTGACCGGTTCTTGTCCACAAAAGCTGTCCCTAGAGAGGAATTTCAACTTGTTGGCATAAGCTCAATGCTTATAGCATCAAAATATGAAGCGAAATGA

Protein sequence

>MS.gene37574.t1

MLMFPNASQGAIAQKPTVPEVIVISSDEEYAEVKEKQVVKRRNLRERSTRRNAKACSSVISARRKASCGIVLEDLVVNIDTADKDNELAVTEYVDDIYAFYKLSEVDVRVHDYMASQPDITAKRRSILVDWWNDVHRTFKLMPETFYLTLNIVDRFLSTKAVPREEFQLVGISSMLIASKYEAK