Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37698.t1 | XP_013469751.1 | 96.2 | 186 | 6 | 1 | 1 | 185 | 1 | 186 | 7.80E-88 | 333.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37698.t1 | P11432 | 73.1 | 197 | 40 | 2 | 1 | 185 | 1 | 196 | 1.5e-68 | 260.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37698.t1 | A0A072VP35 | 96.2 | 186 | 6 | 1 | 1 | 185 | 1 | 186 | 5.6e-88 | 333.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049170 | MS.gene37698 | 0.812035 | 5.19E-51 | -1.69E-46 |
| MS.gene050060 | MS.gene37698 | 0.813451 | 2.54E-51 | -1.69E-46 |
| MS.gene052188 | MS.gene37698 | 0.82516 | 5.45E-54 | -1.69E-46 |
| MS.gene052469 | MS.gene37698 | 0.871136 | 8.60E-67 | -1.69E-46 |
| MS.gene053169 | MS.gene37698 | 0.802324 | 5.92E-49 | -1.69E-46 |
| MS.gene054349 | MS.gene37698 | 0.810378 | 1.19E-50 | -1.69E-46 |
| MS.gene054556 | MS.gene37698 | 0.810996 | 8.73E-51 | -1.69E-46 |
| MS.gene055539 | MS.gene37698 | 0.846962 | 1.56E-59 | -1.69E-46 |
| MS.gene05553 | MS.gene37698 | 0.814612 | 1.41E-51 | -1.69E-46 |
| MS.gene05556 | MS.gene37698 | 0.815997 | 6.94E-52 | -1.69E-46 |
| MS.gene055741 | MS.gene37698 | 0.815866 | 7.42E-52 | -1.69E-46 |
| MS.gene055744 | MS.gene37698 | 0.822399 | 2.42E-53 | -1.69E-46 |
| MS.gene057124 | MS.gene37698 | 0.801705 | 7.94E-49 | -1.69E-46 |
| MS.gene057396 | MS.gene37698 | 0.810666 | 1.03E-50 | -1.69E-46 |
| MS.gene059717 | MS.gene37698 | 0.834291 | 3.26E-56 | -1.69E-46 |
| MS.gene059788 | MS.gene37698 | 0.832002 | 1.21E-55 | -1.69E-46 |
| MS.gene061154 | MS.gene37698 | 0.839952 | 1.16E-57 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37698.t1 | MTR_1g102830 | 96.237 | 186 | 6 | 1 | 1 | 185 | 1 | 186 | 1.13e-127 | 356 |
| MS.gene37698.t1 | MTR_1g102810 | 92.899 | 169 | 12 | 0 | 17 | 185 | 14 | 182 | 1.25e-113 | 321 |
| MS.gene37698.t1 | MTR_1g102780 | 81.006 | 179 | 20 | 1 | 21 | 185 | 22 | 200 | 6.60e-100 | 287 |
| MS.gene37698.t1 | MTR_1g102570 | 80.226 | 177 | 23 | 1 | 21 | 185 | 22 | 198 | 2.76e-98 | 283 |
| MS.gene37698.t1 | MTR_1g102800 | 80.114 | 176 | 23 | 1 | 22 | 185 | 22 | 197 | 5.15e-98 | 282 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37698.t1 | AT3G22840 | 50.500 | 200 | 79 | 4 | 1 | 185 | 1 | 195 | 1.62e-59 | 184 |
| MS.gene37698.t1 | AT4G14690 | 51.852 | 189 | 77 | 3 | 11 | 185 | 5 | 193 | 1.91e-55 | 174 |
Find 52 sgRNAs with CRISPR-Local
Find 75 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGAAGGGATGTTGTTAAAT+TGG | 0.214729 | 1.1:-73784447 | None:intergenic |
| AACCTTCCGTTGATCCTTTC+AGG | 0.216333 | 1.1:-73784976 | None:intergenic |
| GTTGTTGACAGTTGCTTCTT+TGG | 0.263072 | 1.1:+73785107 | MS.gene37698:CDS |
| GCAGATAAGCACAAAGTTTA+CGG | 0.307578 | 1.1:+73784927 | MS.gene37698:intron |
| CACAAAGTTTACGGATTTGA+TGG | 0.311110 | 1.1:+73784936 | MS.gene37698:CDS |
| TTTACGGATTTGATGGCTTT+TGG | 0.325653 | 1.1:+73784943 | MS.gene37698:CDS |
| ACACTAACCCCTTCAAATAA+TGG | 0.328586 | 1.1:-73785132 | None:intergenic |
| ATTCCAGAGCTCTGCATTTG+AGG | 0.357708 | 1.1:-73785181 | None:intergenic |
| GGTTTGTTCGATCAAATATC+TGG | 0.369233 | 1.1:+73785051 | MS.gene37698:CDS |
| TATGTTGGGTTTGATTGCTT+TGG | 0.370659 | 1.1:+73785215 | MS.gene37698:CDS |
| GCAGGCTGCGGTGTCACCTT+TGG | 0.375655 | 1.1:-73784740 | None:intergenic |
| AACGGAAGGTTGGCCATGAT+TGG | 0.385185 | 1.1:+73784988 | MS.gene37698:CDS |
| GGCTGTTACAGAATATGTTA+AGG | 0.394991 | 1.1:+73785236 | MS.gene37698:CDS |
| TCTTTGGTTCCATTATTTGA+AGG | 0.396578 | 1.1:+73785123 | MS.gene37698:CDS |
| CCGTTGATCCTTTCAGGTGC+CGG | 0.400661 | 1.1:-73784970 | None:intergenic |
| GCTGTTACAGAATATGTTAA+GGG | 0.415066 | 1.1:+73785237 | MS.gene37698:CDS |
| TAGCTTGAAAGTTCGATCTA+TGG | 0.429335 | 1.1:+73784498 | MS.gene37698:CDS |
| CTTTGGTTCCATTATTTGAA+GGG | 0.430303 | 1.1:+73785124 | MS.gene37698:CDS |
| TGTATATGCCTAGCTTTAGA+AGG | 0.430319 | 1.1:+73784470 | MS.gene37698:CDS |
| TTGTTAAATTGGTTAACTCT+AGG | 0.465217 | 1.1:-73784436 | None:intergenic |
| TGTCCTCAAATGCAGAGCTC+TGG | 0.486112 | 1.1:+73785178 | MS.gene37698:CDS |
| GAATGGAAGAATTGCTATGT+TGG | 0.488190 | 1.1:+73785200 | MS.gene37698:CDS |
| CTATAAAACTCTATTAAACA+AGG | 0.504429 | 1.1:-73785266 | None:intergenic |
| TGCAGATACAAAACCAATCA+TGG | 0.506046 | 1.1:-73785001 | None:intergenic |
| AATGGAAGAATTGCTATGTT+GGG | 0.510100 | 1.1:+73785201 | MS.gene37698:CDS |
| TTGGTGGGCCGGCACCTGAA+AGG | 0.510772 | 1.1:+73784962 | MS.gene37698:CDS |
| TCTAGGCATGCTAGAAATGC+TGG | 0.515845 | 1.1:-73784419 | None:intergenic |
| TTCGATCAAATATCTGGTGG+TGG | 0.522060 | 1.1:+73785057 | MS.gene37698:CDS |
| CTAGCATTCCTTCTAAAGCT+AGG | 0.532093 | 1.1:-73784478 | None:intergenic |
| TTTGGTTCCATTATTTGAAG+GGG | 0.532185 | 1.1:+73785125 | MS.gene37698:CDS |
| AGTGTGGAGTCTAAGTCAAA+AGG | 0.538159 | 1.1:+73785150 | MS.gene37698:CDS |
| TCAAATGCAGAGCTCTGGAA+TGG | 0.544700 | 1.1:+73785183 | MS.gene37698:CDS |
| CATGCTAGAAATGCTGGTCA+TGG | 0.550746 | 1.1:-73784413 | None:intergenic |
| CACTTGTATACAAGAACCAT+GGG | 0.556587 | 1.1:-73785083 | None:intergenic |
| ATTATTTGAAGGGGTTAGTG+TGG | 0.559171 | 1.1:+73785134 | MS.gene37698:CDS |
| CTCTACTACTCGTTCACCAA+AGG | 0.565802 | 1.1:+73784763 | MS.gene37698:CDS |
| GGTGAACGAGTAGTAGAGGC+AGG | 0.568710 | 1.1:-73784758 | None:intergenic |
| TGAAAGGATCAACGGAAGGT+TGG | 0.570880 | 1.1:+73784978 | MS.gene37698:CDS |
| AAGCTAGGCATATACACAGA+AGG | 0.581115 | 1.1:-73784463 | None:intergenic |
| GGTGGAATTAGCAAAAGGGC+AGG | 0.590541 | 1.1:+73785029 | MS.gene37698:CDS |
| TTGTTCGATCAAATATCTGG+TGG | 0.594040 | 1.1:+73785054 | MS.gene37698:CDS |
| CCGGCACCTGAAAGGATCAA+CGG | 0.601520 | 1.1:+73784970 | MS.gene37698:CDS |
| CGAGTAGTAGAGGCAGGCTG+CGG | 0.601742 | 1.1:-73784752 | None:intergenic |
| AGCTAGGCATATACACAGAA+GGG | 0.602384 | 1.1:-73784462 | None:intergenic |
| GTGGAATTAGCAAAAGGGCA+GGG | 0.608172 | 1.1:+73785030 | MS.gene37698:CDS |
| GAAAGTTCGATCTATGGCTG+AGG | 0.614830 | 1.1:+73784504 | MS.gene37698:CDS |
| ACACTTGTATACAAGAACCA+TGG | 0.618963 | 1.1:-73785084 | None:intergenic |
| CTTTGGTGAACGAGTAGTAG+AGG | 0.621298 | 1.1:-73784762 | None:intergenic |
| AGGGCAGAAAGAGCAACCAA+AGG | 0.630670 | 1.1:+73784724 | MS.gene37698:CDS |
| CACCTGAAAGGATCAACGGA+AGG | 0.644132 | 1.1:+73784974 | MS.gene37698:CDS |
| TATAAAACTCTATTAAACAA+GGG | 0.645194 | 1.1:-73785265 | None:intergenic |
| TATCTGGTGGTGGAATCCCA+TGG | 0.688884 | 1.1:+73785067 | MS.gene37698:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATATATCAAACAAATATTA+AGG | + | chr1.1:73784538-73784557 | MS.gene37698:intron | 10.0% |
| !! | ATCATGATATCATTATATAA+TGG | + | chr1.1:73784905-73784924 | MS.gene37698:intron | 15.0% |
| !!! | GCATATTTAATTTCTATACA+TGG | + | chr1.1:73784584-73784603 | MS.gene37698:intron | 20.0% |
| ! | AATCATAAACGTGTTTGATA+TGG | - | chr1.1:73784618-73784637 | None:intergenic | 25.0% |
| ! | AATGTAAAGAGACATTGTTA+TGG | - | chr1.1:73784696-73784715 | None:intergenic | 25.0% |
| ! | AATGTCTCTTTACATTATTC+AGG | + | chr1.1:73784700-73784719 | MS.gene37698:intron | 25.0% |
| ! | TTGTTAAATTGGTTAACTCT+AGG | - | chr1.1:73784439-73784458 | None:intergenic | 25.0% |
| AATGGAAGAATTGCTATGTT+GGG | + | chr1.1:73785201-73785220 | MS.gene37698:CDS | 30.0% | |
| CTCTTTACATTATTCAGGAA+GGG | + | chr1.1:73784705-73784724 | MS.gene37698:intron | 30.0% | |
| GCTGTTACAGAATATGTTAA+GGG | + | chr1.1:73785237-73785256 | MS.gene37698:CDS | 30.0% | |
| TCTCTTTACATTATTCAGGA+AGG | + | chr1.1:73784704-73784723 | MS.gene37698:intron | 30.0% | |
| TTGCATGACTATAATCTTCT+TGG | - | chr1.1:73784837-73784856 | None:intergenic | 30.0% | |
| TTTATTACTATGCTCACCTT+TGG | - | chr1.1:73784782-73784801 | None:intergenic | 30.0% | |
| ! | CTTTGGTTCCATTATTTGAA+GGG | + | chr1.1:73785124-73785143 | MS.gene37698:CDS | 30.0% |
| ! | TCTTTGGTTCCATTATTTGA+AGG | + | chr1.1:73785123-73785142 | MS.gene37698:CDS | 30.0% |
| ! | TTTGGTTCCATTATTTGAAG+GGG | + | chr1.1:73785125-73785144 | MS.gene37698:CDS | 30.0% |
| !! | GTCATGCAAAGTATTGATTT+CGG | + | chr1.1:73784848-73784867 | MS.gene37698:intron | 30.0% |
| !!! | ATTGGTTTTGTATCTGCAAT+GGG | + | chr1.1:73785006-73785025 | MS.gene37698:CDS | 30.0% |
| !!! | GTCGTTGATTTTGTAAATTC+CGG | + | chr1.1:73784665-73784684 | MS.gene37698:intron | 30.0% |
| ACACTAACCCCTTCAAATAA+TGG | - | chr1.1:73785135-73785154 | None:intergenic | 35.0% | |
| ACACTTGTATACAAGAACCA+TGG | - | chr1.1:73785087-73785106 | None:intergenic | 35.0% | |
| AGACATTGTTATGGATTCTC+CGG | - | chr1.1:73784687-73784706 | None:intergenic | 35.0% | |
| ATCTATGGCTGAGGTAATTA+AGG | + | chr1.1:73784513-73784532 | MS.gene37698:intron | 35.0% | |
| CACAAAGTTTACGGATTTGA+TGG | + | chr1.1:73784936-73784955 | MS.gene37698:CDS | 35.0% | |
| CACTTGTATACAAGAACCAT+GGG | - | chr1.1:73785086-73785105 | None:intergenic | 35.0% | |
| CAGAAGGGATGTTGTTAAAT+TGG | - | chr1.1:73784450-73784469 | None:intergenic | 35.0% | |
| CATAAACGTGTTTGATATGG+TGG | - | chr1.1:73784615-73784634 | None:intergenic | 35.0% | |
| GAATGGAAGAATTGCTATGT+TGG | + | chr1.1:73785200-73785219 | MS.gene37698:CDS | 35.0% | |
| GCAGATAAGCACAAAGTTTA+CGG | + | chr1.1:73784927-73784946 | MS.gene37698:intron | 35.0% | |
| GGCTGTTACAGAATATGTTA+AGG | + | chr1.1:73785236-73785255 | MS.gene37698:CDS | 35.0% | |
| TAGCTTGAAAGTTCGATCTA+TGG | + | chr1.1:73784498-73784517 | MS.gene37698:CDS | 35.0% | |
| TATGTTGGGTTTGATTGCTT+TGG | + | chr1.1:73785215-73785234 | MS.gene37698:CDS | 35.0% | |
| TGCAGATACAAAACCAATCA+TGG | - | chr1.1:73785004-73785023 | None:intergenic | 35.0% | |
| TTGTTCGATCAAATATCTGG+TGG | + | chr1.1:73785054-73785073 | MS.gene37698:CDS | 35.0% | |
| ! | ATTATTTGAAGGGGTTAGTG+TGG | + | chr1.1:73785134-73785153 | MS.gene37698:CDS | 35.0% |
| ! | GGTTTGTTCGATCAAATATC+TGG | + | chr1.1:73785051-73785070 | MS.gene37698:CDS | 35.0% |
| !! | TGTATATGCCTAGCTTTAGA+AGG | + | chr1.1:73784470-73784489 | MS.gene37698:CDS | 35.0% |
| !! | TTTACGGATTTGATGGCTTT+TGG | + | chr1.1:73784943-73784962 | MS.gene37698:CDS | 35.0% |
| !!! | GATTGGTTTTGTATCTGCAA+TGG | + | chr1.1:73785005-73785024 | MS.gene37698:CDS | 35.0% |
| !!! | TTGGTTTTGTATCTGCAATG+GGG | + | chr1.1:73785007-73785026 | MS.gene37698:CDS | 35.0% |
| AAGCTAGGCATATACACAGA+AGG | - | chr1.1:73784466-73784485 | None:intergenic | 40.0% | |
| AGCTAGGCATATACACAGAA+GGG | - | chr1.1:73784465-73784484 | None:intergenic | 40.0% | |
| AGTGTGGAGTCTAAGTCAAA+AGG | + | chr1.1:73785150-73785169 | MS.gene37698:CDS | 40.0% | |
| GTTGTTGACAGTTGCTTCTT+TGG | + | chr1.1:73785107-73785126 | MS.gene37698:CDS | 40.0% | |
| TTCGATCAAATATCTGGTGG+TGG | + | chr1.1:73785057-73785076 | MS.gene37698:CDS | 40.0% | |
| !! | CTAGCATTCCTTCTAAAGCT+AGG | - | chr1.1:73784481-73784500 | None:intergenic | 40.0% |
| !!! | TGGTTTTGTATCTGCAATGG+GGG | + | chr1.1:73785008-73785027 | MS.gene37698:CDS | 40.0% |
| AACCTTCCGTTGATCCTTTC+AGG | - | chr1.1:73784979-73784998 | None:intergenic | 45.0% | |
| ATGGGGGTGGAATTAGCAAA+AGG | + | chr1.1:73785024-73785043 | MS.gene37698:CDS | 45.0% | |
| ATTCCAGAGCTCTGCATTTG+AGG | - | chr1.1:73785184-73785203 | None:intergenic | 45.0% | |
| CATGCTAGAAATGCTGGTCA+TGG | - | chr1.1:73784416-73784435 | None:intergenic | 45.0% | |
| CTCTACTACTCGTTCACCAA+AGG | + | chr1.1:73784763-73784782 | MS.gene37698:CDS | 45.0% | |
| CTTTGGTGAACGAGTAGTAG+AGG | - | chr1.1:73784765-73784784 | None:intergenic | 45.0% | |
| GAAAGTTCGATCTATGGCTG+AGG | + | chr1.1:73784504-73784523 | MS.gene37698:CDS | 45.0% | |
| GTGGAATTAGCAAAAGGGCA+GGG | + | chr1.1:73785030-73785049 | MS.gene37698:CDS | 45.0% | |
| TCTAGGCATGCTAGAAATGC+TGG | - | chr1.1:73784422-73784441 | None:intergenic | 45.0% | |
| TGAAAGGATCAACGGAAGGT+TGG | + | chr1.1:73784978-73784997 | MS.gene37698:CDS | 45.0% | |
| TGGGGGTGGAATTAGCAAAA+GGG | + | chr1.1:73785025-73785044 | MS.gene37698:CDS | 45.0% | |
| ! | TTTTGTATCTGCAATGGGGG+TGG | + | chr1.1:73785011-73785030 | MS.gene37698:CDS | 45.0% |
| !! | ACGGATTTGATGGCTTTTGG+TGG | + | chr1.1:73784946-73784965 | MS.gene37698:CDS | 45.0% |
| !! | CGGATTTGATGGCTTTTGGT+GGG | + | chr1.1:73784947-73784966 | MS.gene37698:CDS | 45.0% |
| !! | TCAAATGCAGAGCTCTGGAA+TGG | + | chr1.1:73785183-73785202 | MS.gene37698:CDS | 45.0% |
| AGGGCAGAAAGAGCAACCAA+AGG | + | chr1.1:73784724-73784743 | MS.gene37698:CDS | 50.0% | |
| CACCTGAAAGGATCAACGGA+AGG | + | chr1.1:73784974-73784993 | MS.gene37698:CDS | 50.0% | |
| GGTGGAATTAGCAAAAGGGC+AGG | + | chr1.1:73785029-73785048 | MS.gene37698:CDS | 50.0% | |
| TGTCCTCAAATGCAGAGCTC+TGG | + | chr1.1:73785178-73785197 | MS.gene37698:CDS | 50.0% | |
| ! | AACGGAAGGTTGGCCATGAT+TGG | + | chr1.1:73784988-73785007 | MS.gene37698:CDS | 50.0% |
| !! | TATCTGGTGGTGGAATCCCA+TGG | + | chr1.1:73785067-73785086 | MS.gene37698:CDS | 50.0% |
| !! | TTTGATGGCTTTTGGTGGGC+CGG | + | chr1.1:73784951-73784970 | MS.gene37698:CDS | 50.0% |
| CCGGCACCTGAAAGGATCAA+CGG | + | chr1.1:73784970-73784989 | MS.gene37698:CDS | 55.0% | |
| GGTGAACGAGTAGTAGAGGC+AGG | - | chr1.1:73784761-73784780 | None:intergenic | 55.0% | |
| ! | CCGTTGATCCTTTCAGGTGC+CGG | - | chr1.1:73784973-73784992 | None:intergenic | 55.0% |
| CGAGTAGTAGAGGCAGGCTG+CGG | - | chr1.1:73784755-73784774 | None:intergenic | 60.0% | |
| ! | TTGGTGGGCCGGCACCTGAA+AGG | + | chr1.1:73784962-73784981 | MS.gene37698:CDS | 65.0% |
| !! | GCAGGCTGCGGTGTCACCTT+TGG | - | chr1.1:73784743-73784762 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 73784376 | 73785275 | 73784376 | ID=MS.gene37698 |
| chr1.1 | mRNA | 73784376 | 73785275 | 73784376 | ID=MS.gene37698.t1;Parent=MS.gene37698 |
| chr1.1 | exon | 73784376 | 73784525 | 73784376 | ID=MS.gene37698.t1.exon1;Parent=MS.gene37698.t1 |
| chr1.1 | CDS | 73784376 | 73784525 | 73784376 | ID=cds.MS.gene37698.t1;Parent=MS.gene37698.t1 |
| chr1.1 | exon | 73784722 | 73784784 | 73784722 | ID=MS.gene37698.t1.exon2;Parent=MS.gene37698.t1 |
| chr1.1 | CDS | 73784722 | 73784784 | 73784722 | ID=cds.MS.gene37698.t1;Parent=MS.gene37698.t1 |
| chr1.1 | exon | 73784931 | 73785275 | 73784931 | ID=MS.gene37698.t1.exon3;Parent=MS.gene37698.t1 |
| chr1.1 | CDS | 73784931 | 73785275 | 73784931 | ID=cds.MS.gene37698.t1;Parent=MS.gene37698.t1 |
| Gene Sequence |
| Protein sequence |
