AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene38678


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene38678.t1 MTR_3g102500 98.137 161 3 0 1 161 1 161 1.99e-110 311
MS.gene38678.t1 MTR_1g018710 57.851 121 42 3 1 121 1 112 1.34e-37 125
MS.gene38678.t1 MTR_1g072250 45.455 88 47 1 36 122 17 104 4.73e-19 83.2
MS.gene38678.t1 MTR_1g105040 39.474 114 64 3 26 136 6 117 3.11e-17 78.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene38678.t1 AT1G47200 62.500 96 35 1 26 121 50 144 1.08e-37 127
MS.gene38678.t1 AT5G43070 47.015 134 62 2 1 126 1 133 2.77e-34 118
MS.gene38678.t1 AT5G27940 40.385 104 56 2 27 127 41 141 2.94e-21 84.7
MS.gene38678.t1 AT3G63130 40.000 100 58 2 36 133 17 116 2.16e-16 75.9
MS.gene38678.t1 AT3G63130 40.000 100 58 2 36 133 17 116 2.16e-16 75.9
MS.gene38678.t1 AT5G19320 34.951 103 66 1 27 128 7 109 3.16e-14 69.3
MS.gene38678.t1 AT5G19320 34.951 103 66 1 27 128 7 109 3.16e-14 69.3

Find 65 sgRNAs with CRISPR-Local

Find 72 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATCTTCTTACTGATTTCCT+TGG 0.339205 3.3:+84699507 None:intergenic
GACGGCGTCGCGGGTGCGTT+GGG 0.359562 3.3:+84699708 None:intergenic
AACGGTAGGTCTAGCCTTAA+CGG 0.377656 3.3:+84699477 None:intergenic
AAGGACGGAGGGAGTGGTTA+GGG 0.381916 3.3:+84699666 None:intergenic
TATGCTGAATCCGGCGGAGG+GGG 0.384348 3.3:+84699741 None:intergenic
GAAGATGCCTTCTCTGTTGC+CGG 0.386460 3.3:-84699583 MS.gene38678:CDS
AAAGGACGGAGGGAGTGGTT+AGG 0.399888 3.3:+84699665 None:intergenic
TGATTCTAGCGCCGTTGATA+AGG 0.405504 3.3:-84699455 MS.gene38678:CDS
TCAATCCCGTCACCACCTTC+AGG 0.411209 3.3:+84699547 None:intergenic
CCTCCGCCGGATTCAGCATA+TGG 0.414915 3.3:-84699738 MS.gene38678:CDS
GGGCAATGTGCCGTAGCGTT+TGG 0.420216 3.3:+84699642 None:intergenic
GTTAGAGTCCGATGAGGTTA+CGG 0.425356 3.3:+84699804 None:intergenic
CCATATGCTGAATCCGGCGG+AGG 0.431909 3.3:+84699738 None:intergenic
GGCGTCGCGGGTGCGTTGGG+TGG 0.444388 3.3:+84699711 None:intergenic
TGACGGCGTCGCGGGTGCGT+TGG 0.444623 3.3:+84699707 None:intergenic
TGACGGGATTGAGATCCTTC+AGG 0.459478 3.3:-84699536 MS.gene38678:CDS
CATATGCTGAATCCGGCGGA+GGG 0.462008 3.3:+84699739 None:intergenic
GCGAGTGATGACGGCGTCGC+GGG 0.464948 3.3:+84699699 None:intergenic
GCGTCGCGGGTGCGTTGGGT+GGG 0.477005 3.3:+84699712 None:intergenic
GGGTGGCCATATGCTGAATC+CGG 0.477190 3.3:+84699732 None:intergenic
AGCGGAGGCGGCTTCGTCGG+AGG 0.481716 3.3:+84699621 None:intergenic
GCCGTTCCTGAAGGTGGTGA+CGG 0.483359 3.3:-84699553 MS.gene38678:CDS
CCGTTCCTGAAGGTGGTGAC+GGG 0.486136 3.3:-84699552 MS.gene38678:CDS
TCGCGGGTGCGTTGGGTGGG+TGG 0.491919 3.3:+84699715 None:intergenic
AGCAGCGGAGGCGGCTTCGT+CGG 0.495524 3.3:+84699618 None:intergenic
TTCAGGAACGGCGGAGTCAC+CGG 0.495877 3.3:+84699564 None:intergenic
GAAGATGCTTGAAACCGTTA+AGG 0.498552 3.3:-84699491 MS.gene38678:CDS
GCGGAGGCGGCTTCGTCGGA+GGG 0.515254 3.3:+84699622 None:intergenic
ATGCTGAATCCGGCGGAGGG+GGG 0.517309 3.3:+84699742 None:intergenic
CTCGATCTGACGAGCAGCGG+AGG 0.518920 3.3:+84699606 None:intergenic
TTTGGAAAGGACGGAGGGAG+TGG 0.526920 3.3:+84699660 None:intergenic
GGTGACTCCGCCGTTCCTGA+AGG 0.545409 3.3:-84699562 MS.gene38678:CDS
TTCGATTAAGCGAGTGATGA+CGG 0.547064 3.3:+84699690 None:intergenic
ATATGCTGAATCCGGCGGAG+GGG 0.550066 3.3:+84699740 None:intergenic
GATCCTTCAGGTGTATTCCA+AGG 0.552260 3.3:-84699524 MS.gene38678:CDS
GTTCCACCACCGCAACAAAA+CGG 0.560480 3.3:-84699778 MS.gene38678:CDS
GATCTGACGAGCAGCGGAGG+CGG 0.560720 3.3:+84699609 None:intergenic
GGAGTCACCGGCAACAGAGA+AGG 0.560862 3.3:+84699576 None:intergenic
TACGGTTGTGACTTCTGAGT+CGG 0.571822 3.3:+84699822 None:intergenic
CCCGTCACCACCTTCAGGAA+CGG 0.575190 3.3:+84699552 None:intergenic
TGATAAGGACGCTGCTGCTG+TGG 0.577989 3.3:-84699440 MS.gene38678:CDS
AGCAGCAGCGTCCTTATCAA+CGG 0.578380 3.3:+84699444 None:intergenic
CTCCGCTGCTCGTCAGATCG+AGG 0.582950 3.3:-84699605 MS.gene38678:CDS
GCCGTAGCGTTTGGAAAGGA+CGG 0.591975 3.3:+84699651 None:intergenic
AGTCTCCGTCTCGATCTTCG+CGG 0.594787 3.3:+84699372 None:intergenic
GACTCCGCCGTTCCTGAAGG+TGG 0.599113 3.3:-84699559 MS.gene38678:CDS
TCAGCAGCATCCTCTGCAGA+AGG 0.602802 3.3:+84699397 None:intergenic
ATCAACGGCGCTAGAATCAA+CGG 0.603734 3.3:+84699459 None:intergenic
TTTCCTTGGAATACACCTGA+AGG 0.605904 3.3:+84699521 None:intergenic
TTCCTCGATCTGACGAGCAG+CGG 0.613011 3.3:+84699603 None:intergenic
AGCGAGTGATGACGGCGTCG+CGG 0.615225 3.3:+84699698 None:intergenic
AGTCACAACCGTAACCTCAT+CGG 0.620023 3.3:-84699812 MS.gene38678:CDS
TGGCCATATGCTGAATCCGG+CGG 0.621199 3.3:+84699735 None:intergenic
TGATGATCCTCCTTCTGCAG+AGG 0.625146 3.3:-84699407 MS.gene38678:CDS
ATGTGCCGTAGCGTTTGGAA+AGG 0.630318 3.3:+84699647 None:intergenic
GCAGCATCCTCTGCAGAAGG+AGG 0.632854 3.3:+84699400 None:intergenic
TCCGTCCTTTCCAAACGCTA+CGG 0.635061 3.3:-84699652 MS.gene38678:CDS
TAGCGTTTGGAAAGGACGGA+GGG 0.638385 3.3:+84699655 None:intergenic
GAAGGAGGATCATCAACGGT+CGG 0.638649 3.3:+84699415 None:intergenic
TGCAGAAGGAGGATCATCAA+CGG 0.659742 3.3:+84699411 None:intergenic
GTCACCACCTTCAGGAACGG+CGG 0.675663 3.3:+84699555 None:intergenic
GTAGCGTTTGGAAAGGACGG+AGG 0.678784 3.3:+84699654 None:intergenic
TGAATCCGCGAAGATCGAGA+CGG 0.689865 3.3:-84699377 MS.gene38678:CDS
ACGGCGCTAGAATCAACGGT+AGG 0.692601 3.3:+84699463 None:intergenic
AACGGAGTTAGAGTCCGATG+AGG 0.740548 3.3:+84699798 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CATCTTCTTACTGATTTCCT+TGG + chr3.3:84699685-84699704 None:intergenic 35.0%
GAAGATGCTTGAAACCGTTA+AGG - chr3.3:84699698-84699717 MS.gene38678:CDS 40.0%
TTCGATTAAGCGAGTGATGA+CGG + chr3.3:84699502-84699521 None:intergenic 40.0%
TTTCCTTGGAATACACCTGA+AGG + chr3.3:84699671-84699690 None:intergenic 40.0%
AGTCACAACCGTAACCTCAT+CGG - chr3.3:84699377-84699396 MS.gene38678:CDS 45.0%
ATCAACGGCGCTAGAATCAA+CGG + chr3.3:84699733-84699752 None:intergenic 45.0%
GATCCTTCAGGTGTATTCCA+AGG - chr3.3:84699665-84699684 MS.gene38678:CDS 45.0%
GTTAGAGTCCGATGAGGTTA+CGG + chr3.3:84699388-84699407 None:intergenic 45.0%
TACGGTTGTGACTTCTGAGT+CGG + chr3.3:84699370-84699389 None:intergenic 45.0%
TGATTCTAGCGCCGTTGATA+AGG - chr3.3:84699734-84699753 MS.gene38678:CDS 45.0%
TGCAGAAGGAGGATCATCAA+CGG + chr3.3:84699781-84699800 None:intergenic 45.0%
!! AACGGTAGGTCTAGCCTTAA+CGG + chr3.3:84699715-84699734 None:intergenic 45.0%
AACGGAGTTAGAGTCCGATG+AGG + chr3.3:84699394-84699413 None:intergenic 50.0%
AGCAGCAGCGTCCTTATCAA+CGG + chr3.3:84699748-84699767 None:intergenic 50.0%
ATGTGCCGTAGCGTTTGGAA+AGG + chr3.3:84699545-84699564 None:intergenic 50.0%
GAAGATGCCTTCTCTGTTGC+CGG - chr3.3:84699606-84699625 MS.gene38678:CDS 50.0%
GAAGGAGGATCATCAACGGT+CGG + chr3.3:84699777-84699796 None:intergenic 50.0%
GTTCCACCACCGCAACAAAA+CGG - chr3.3:84699411-84699430 MS.gene38678:CDS 50.0%
TAGCGTTTGGAAAGGACGGA+GGG + chr3.3:84699537-84699556 None:intergenic 50.0%
TCCGTCCTTTCCAAACGCTA+CGG - chr3.3:84699537-84699556 MS.gene38678:CDS 50.0%
TGAATCCGCGAAGATCGAGA+CGG - chr3.3:84699812-84699831 MS.gene38678:CDS 50.0%
TGACGGGATTGAGATCCTTC+AGG - chr3.3:84699653-84699672 MS.gene38678:CDS 50.0%
TGATGATCCTCCTTCTGCAG+AGG - chr3.3:84699782-84699801 MS.gene38678:CDS 50.0%
!! GTTTGGTCGCCGTTTTGTTG+CGG + chr3.3:84699423-84699442 None:intergenic 50.0%
!!! GTTTTGTTGCGGTGGTGGAA+CGG + chr3.3:84699412-84699431 None:intergenic 50.0%
AAAGGACGGAGGGAGTGGTT+AGG + chr3.3:84699527-84699546 None:intergenic 55.0%
AAGGACGGAGGGAGTGGTTA+GGG + chr3.3:84699526-84699545 None:intergenic 55.0%
ACGGCGCTAGAATCAACGGT+AGG + chr3.3:84699729-84699748 None:intergenic 55.0%
AGTCTCCGTCTCGATCTTCG+CGG + chr3.3:84699820-84699839 None:intergenic 55.0%
ATATGCTGAATCCGGCGGAG+GGG + chr3.3:84699452-84699471 None:intergenic 55.0%
CATATGCTGAATCCGGCGGA+GGG + chr3.3:84699453-84699472 None:intergenic 55.0%
GCCGTAGCGTTTGGAAAGGA+CGG + chr3.3:84699541-84699560 None:intergenic 55.0%
GGGTGGCCATATGCTGAATC+CGG + chr3.3:84699460-84699479 None:intergenic 55.0%
GTAGCGTTTGGAAAGGACGG+AGG + chr3.3:84699538-84699557 None:intergenic 55.0%
TCAATCCCGTCACCACCTTC+AGG + chr3.3:84699645-84699664 None:intergenic 55.0%
TCAGCAGCATCCTCTGCAGA+AGG + chr3.3:84699795-84699814 None:intergenic 55.0%
TGATAAGGACGCTGCTGCTG+TGG - chr3.3:84699749-84699768 MS.gene38678:CDS 55.0%
TGGCCATATGCTGAATCCGG+CGG + chr3.3:84699457-84699476 None:intergenic 55.0%
TTCCTCGATCTGACGAGCAG+CGG + chr3.3:84699589-84699608 None:intergenic 55.0%
TTTGGAAAGGACGGAGGGAG+TGG + chr3.3:84699532-84699551 None:intergenic 55.0%
CCATATGCTGAATCCGGCGG+AGG + chr3.3:84699454-84699473 None:intergenic 60.0%
CCCGTCACCACCTTCAGGAA+CGG + chr3.3:84699640-84699659 None:intergenic 60.0%
CCGTTCCTGAAGGTGGTGAC+GGG - chr3.3:84699637-84699656 MS.gene38678:CDS 60.0%
CCTCCGCCGGATTCAGCATA+TGG - chr3.3:84699451-84699470 MS.gene38678:CDS 60.0%
GCAGCATCCTCTGCAGAAGG+AGG + chr3.3:84699792-84699811 None:intergenic 60.0%
GCCGTTCCTGAAGGTGGTGA+CGG - chr3.3:84699636-84699655 MS.gene38678:CDS 60.0%
GGAGTCACCGGCAACAGAGA+AGG + chr3.3:84699616-84699635 None:intergenic 60.0%
GGGCAATGTGCCGTAGCGTT+TGG + chr3.3:84699550-84699569 None:intergenic 60.0%
GTCACCACCTTCAGGAACGG+CGG + chr3.3:84699637-84699656 None:intergenic 60.0%
TATGCTGAATCCGGCGGAGG+GGG + chr3.3:84699451-84699470 None:intergenic 60.0%
TTCAGGAACGGCGGAGTCAC+CGG + chr3.3:84699628-84699647 None:intergenic 60.0%
!! TCGCCGTTTTGTTGCGGTGG+TGG + chr3.3:84699417-84699436 None:intergenic 60.0%
!! TGGTCGCCGTTTTGTTGCGG+TGG + chr3.3:84699420-84699439 None:intergenic 60.0%
ATGCTGAATCCGGCGGAGGG+GGG + chr3.3:84699450-84699469 None:intergenic 65.0%
CAAACCAAACCCCCCTCCGC+CGG - chr3.3:84699438-84699457 MS.gene38678:CDS 65.0%
CTCCGCTGCTCGTCAGATCG+AGG - chr3.3:84699584-84699603 MS.gene38678:CDS 65.0%
CTCGATCTGACGAGCAGCGG+AGG + chr3.3:84699586-84699605 None:intergenic 65.0%
GACTCCGCCGTTCCTGAAGG+TGG - chr3.3:84699630-84699649 MS.gene38678:CDS 65.0%
GATCTGACGAGCAGCGGAGG+CGG + chr3.3:84699583-84699602 None:intergenic 65.0%
GGTGACTCCGCCGTTCCTGA+AGG - chr3.3:84699627-84699646 MS.gene38678:CDS 65.0%
!! AGCGAGTGATGACGGCGTCG+CGG + chr3.3:84699494-84699513 None:intergenic 65.0%
AGCAGCGGAGGCGGCTTCGT+CGG + chr3.3:84699574-84699593 None:intergenic 70.0%
GAATCCGGCGGAGGGGGGTT+TGG + chr3.3:84699445-84699464 None:intergenic 70.0%
! CGGCGGAGGGGGGTTTGGTT+TGG + chr3.3:84699440-84699459 None:intergenic 70.0%
!! GCGAGTGATGACGGCGTCGC+GGG + chr3.3:84699493-84699512 None:intergenic 70.0%
AGCGGAGGCGGCTTCGTCGG+AGG + chr3.3:84699571-84699590 None:intergenic 75.0%
GACGGCGTCGCGGGTGCGTT+GGG + chr3.3:84699484-84699503 None:intergenic 75.0%
GCGGAGGCGGCTTCGTCGGA+GGG + chr3.3:84699570-84699589 None:intergenic 75.0%
GCGTCGCGGGTGCGTTGGGT+GGG + chr3.3:84699480-84699499 None:intergenic 75.0%
TCGCGGGTGCGTTGGGTGGG+TGG + chr3.3:84699477-84699496 None:intergenic 75.0%
TGACGGCGTCGCGGGTGCGT+TGG + chr3.3:84699485-84699504 None:intergenic 75.0%
! GGCGTCGCGGGTGCGTTGGG+TGG + chr3.3:84699481-84699500 None:intergenic 80.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 84699363 84699848 84699363 ID=MS.gene38678
chr3.3 mRNA 84699363 84699848 84699363 ID=MS.gene38678.t1;Parent=MS.gene38678
chr3.3 exon 84699363 84699848 84699363 ID=MS.gene38678.t1.exon1;Parent=MS.gene38678.t1
chr3.3 CDS 84699363 84699848 84699363 ID=cds.MS.gene38678.t1;Parent=MS.gene38678.t1
Gene Sequence

>MS.gene38678

ATGTCCGACTCAGAAGTCACAACCGTAACCTCATCGGACTCTAACTCCGTTCCACCACCGCAACAAAACGGCGACCAAACCAAACCCCCCTCCGCCGGATTCAGCATATGGCCACCCACCCAACGCACCCGCGACGCCGTCATCACTCGCTTAATCGAAACCCTAACCACTCCCTCCGTCCTTTCCAAACGCTACGGCACATTGCCCTCCGACGAAGCCGCCTCCGCTGCTCGTCAGATCGAGGAAGATGCCTTCTCTGTTGCCGGTGACTCCGCCGTTCCTGAAGGTGGTGACGGGATTGAGATCCTTCAGGTGTATTCCAAGGAAATCAGTAAGAAGATGCTTGAAACCGTTAAGGCTAGACCTACCGTTGATTCTAGCGCCGTTGATAAGGACGCTGCTGCTGTGGCACCGACCGTTGATGATCCTCCTTCTGCAGAGGATGCTGCTGAATCCGCGAAGATCGAGACGGAGACTGAAACCTGA

Protein sequence

>MS.gene38678.t1

MSDSEVTTVTSSDSNSVPPPQQNGDQTKPPSAGFSIWPPTQRTRDAVITRLIETLTTPSVLSKRYGTLPSDEAASAARQIEEDAFSVAGDSAVPEGGDGIEILQVYSKEISKKMLETVKARPTVDSSAVDKDAAAVAPTVDDPPSAEDAAESAKIETETET