Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38763.t1 | XP_003625004.1 | 96.8 | 157 | 5 | 0 | 1 | 157 | 1 | 157 | 4.00E-77 | 297.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38763.t1 | Q2HW51 | 96.8 | 157 | 5 | 0 | 1 | 157 | 1 | 157 | 2.9e-77 | 297.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene38763 | 0.839459 | 1.56E-57 | -1.69E-46 |
| MS.gene049698 | MS.gene38763 | 0.810456 | 1.14E-50 | -1.69E-46 |
| MS.gene049711 | MS.gene38763 | 0.833577 | 4.92E-56 | -1.69E-46 |
| MS.gene050807 | MS.gene38763 | 0.805171 | 1.52E-49 | -1.69E-46 |
| MS.gene051247 | MS.gene38763 | 0.804588 | 2.01E-49 | -1.69E-46 |
| MS.gene051719 | MS.gene38763 | 0.81376 | 2.17E-51 | -1.69E-46 |
| MS.gene051720 | MS.gene38763 | 0.825252 | 5.19E-54 | -1.69E-46 |
| MS.gene052516 | MS.gene38763 | 0.807411 | 5.12E-50 | -1.69E-46 |
| MS.gene053144 | MS.gene38763 | 0.83588 | 1.30E-56 | -1.69E-46 |
| MS.gene053581 | MS.gene38763 | 0.821878 | 3.19E-53 | -1.69E-46 |
| MS.gene054584 | MS.gene38763 | 0.82611 | 3.25E-54 | -1.69E-46 |
| MS.gene05458 | MS.gene38763 | 0.821368 | 4.19E-53 | -1.69E-46 |
| MS.gene055144 | MS.gene38763 | 0.861236 | 1.18E-63 | -1.69E-46 |
| MS.gene055466 | MS.gene38763 | 0.809182 | 2.15E-50 | -1.69E-46 |
| MS.gene055468 | MS.gene38763 | 0.816238 | 6.13E-52 | -1.69E-46 |
| MS.gene055469 | MS.gene38763 | 0.803329 | 3.67E-49 | -1.69E-46 |
| MS.gene055537 | MS.gene38763 | 0.824985 | 6.00E-54 | -1.69E-46 |
| MS.gene055580 | MS.gene38763 | -0.814745 | 1.32E-51 | -1.69E-46 |
| MS.gene056043 | MS.gene38763 | -0.812112 | 4.99E-51 | -1.69E-46 |
| MS.gene058339 | MS.gene38763 | 0.851323 | 9.57E-61 | -1.69E-46 |
| MS.gene05847 | MS.gene38763 | 0.818878 | 1.56E-52 | -1.69E-46 |
| MS.gene058852 | MS.gene38763 | 0.810309 | 1.23E-50 | -1.69E-46 |
| MS.gene058909 | MS.gene38763 | 0.834007 | 3.84E-56 | -1.69E-46 |
| MS.gene058912 | MS.gene38763 | 0.831038 | 2.09E-55 | -1.69E-46 |
| MS.gene05894 | MS.gene38763 | 0.805747 | 1.15E-49 | -1.69E-46 |
| MS.gene060429 | MS.gene38763 | 0.809248 | 2.08E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38763.t1 | MTR_7g089940 | 96.815 | 157 | 5 | 0 | 1 | 157 | 1 | 157 | 3.84e-107 | 302 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38763.t1 | AT2G41200 | 44.444 | 162 | 84 | 3 | 1 | 157 | 1 | 161 | 2.64e-40 | 133 |
Find 34 sgRNAs with CRISPR-Local
Find 67 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCTCAAGTTCCTCCGCTTT+GGG | 0.168009 | 7.4:+18888513 | None:intergenic |
| CCTACTCCTCGGTAGAATTC+TGG | 0.227742 | 7.4:+18888717 | None:intergenic |
| TCATAGAATTTATCTATAAT+TGG | 0.255004 | 7.4:+18888771 | None:intergenic |
| CTTCTCAAGTTCCTCCGCTT+TGG | 0.271530 | 7.4:+18888512 | None:intergenic |
| GATTATGAAAGAGATGATAA+AGG | 0.392683 | 7.4:-18888027 | MS.gene38763:CDS |
| GTGTTAATTTCTTCAAAGAT+TGG | 0.394794 | 7.4:+18888933 | None:intergenic |
| GAAAAGAAGGCCAAGGAACT+TGG | 0.400216 | 7.4:-18888795 | MS.gene38763:CDS |
| AAAGAGATGATAAAGGAATC+AGG | 0.421896 | 7.4:-18888020 | MS.gene38763:CDS |
| TCATCTCTTTCATAATCTCC+TGG | 0.424347 | 7.4:+18888033 | None:intergenic |
| ATTGACAAAAGCAGAATTCC+AGG | 0.425391 | 7.4:-18888051 | MS.gene38763:CDS |
| CTACTCCTCGGTAGAATTCT+GGG | 0.431018 | 7.4:+18888718 | None:intergenic |
| TATAATTGGTCCAAGTTCCT+TGG | 0.449567 | 7.4:+18888785 | None:intergenic |
| GAAGCACTCTTATACATCTT+CGG | 0.498360 | 7.4:-18887975 | MS.gene38763:CDS |
| ACTGTGACTGAAGTGATCCC+TGG | 0.504884 | 7.4:+18887882 | None:intergenic |
| GTATTGTAGGAACACCATAA+AGG | 0.522447 | 7.4:-18888086 | MS.gene38763:intron |
| AATTCATTTGGAATTGCTTG+AGG | 0.522690 | 7.4:+18887912 | None:intergenic |
| CTGTTTGTTAAGCAAAGAGT+GGG | 0.530709 | 7.4:-18887936 | MS.gene38763:CDS |
| TGCAGGAACTGATGAAAAGA+AGG | 0.536275 | 7.4:-18888808 | MS.gene38763:intron |
| CCAGAATTCTACCGAGGAGT+AGG | 0.546883 | 7.4:-18888717 | MS.gene38763:CDS |
| ATAAAGGAATCAGGATTCAC+TGG | 0.550946 | 7.4:-18888011 | MS.gene38763:CDS |
| TCTCAAGTTCCTCCGCTTTG+GGG | 0.555223 | 7.4:+18888514 | None:intergenic |
| CGACTATCTCGCCTACTCCT+CGG | 0.559020 | 7.4:+18888706 | None:intergenic |
| ATTCACTGGCGTGGGAGCAA+AGG | 0.560009 | 7.4:-18887997 | MS.gene38763:CDS |
| GAATCAGGATTCACTGGCGT+GGG | 0.566503 | 7.4:-18888005 | MS.gene38763:CDS |
| CAAAGCGGAGGAACTTGAGA+AGG | 0.590274 | 7.4:-18888511 | MS.gene38763:CDS |
| TCTGTTTGTTAAGCAAAGAG+TGG | 0.593550 | 7.4:-18887937 | MS.gene38763:CDS |
| GGAATCAGGATTCACTGGCG+TGG | 0.595088 | 7.4:-18888006 | MS.gene38763:CDS |
| AACTGATGAAAAGAAGGCCA+AGG | 0.610768 | 7.4:-18888802 | MS.gene38763:CDS |
| GATCAAACTCCCCAAAGCGG+AGG | 0.639003 | 7.4:-18888523 | MS.gene38763:CDS |
| TTAACAAACAGAGCAGTCAC+AGG | 0.646447 | 7.4:+18887948 | None:intergenic |
| TCTTTGAAGAAATTAACACC+AGG | 0.679978 | 7.4:-18888929 | MS.gene38763:intron |
| GACTTCCCAGAATTCTACCG+AGG | 0.719997 | 7.4:-18888723 | MS.gene38763:CDS |
| TCAGATCAAACTCCCCAAAG+CGG | 0.722883 | 7.4:-18888526 | MS.gene38763:CDS |
| TGTTTGTTAAGCAAAGAGTG+GGG | 0.726737 | 7.4:-18887935 | MS.gene38763:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TATGTTTAAAATTGTAATTA+TGG | - | chr7.4:18888365-18888384 | MS.gene38763:intron | 10.0% |
| !!! | TTTTTTTTTTAATGTATTGT+AGG | - | chr7.4:18888690-18888709 | MS.gene38763:intron | 10.0% |
| !! | TCATAGAATTTATCTATAAT+TGG | + | chr7.4:18888021-18888040 | None:intergenic | 15.0% |
| !!! | AAGAGAAATTTAAGTTTTTT+AGG | + | chr7.4:18888630-18888649 | None:intergenic | 15.0% |
| !!! | ATTTTATTTATTTATCACCT+AGG | - | chr7.4:18888172-18888191 | MS.gene38763:intron | 15.0% |
| !!! | TATCTCATTATCTTATATTT+TGG | - | chr7.4:18888326-18888345 | MS.gene38763:intron | 15.0% |
| !! | AAGATATAAAAAATGCTTCA+AGG | - | chr7.4:18888043-18888062 | MS.gene38763:CDS | 20.0% |
| !! | AGATATAAAAAATGCTTCAA+GGG | - | chr7.4:18888044-18888063 | MS.gene38763:CDS | 20.0% |
| !! | TAAATTTCTCTTATGTAACA+TGG | - | chr7.4:18888638-18888657 | MS.gene38763:intron | 20.0% |
| !!! | TTTTAATGAAAAATTGATGC+AGG | - | chr7.4:18887964-18887983 | MS.gene38763:CDS | 20.0% |
| ! | GATTATGAAAGAGATGATAA+AGG | - | chr7.4:18888762-18888781 | MS.gene38763:CDS | 25.0% |
| ! | GTGTTAATTTCTTCAAAGAT+TGG | + | chr7.4:18887859-18887878 | None:intergenic | 25.0% |
| !! | CAAATGAATTTTTCATTCCA+GGG | - | chr7.4:18888890-18888909 | MS.gene38763:intron | 25.0% |
| !! | TACAACTTTTCATCATTCTA+TGG | - | chr7.4:18888210-18888229 | MS.gene38763:intron | 25.0% |
| !!! | ATTCCTTGTTTTTTCCTTTA+TGG | + | chr7.4:18888720-18888739 | None:intergenic | 25.0% |
| !!! | ATTTAAGTTTTTTAGGCCTA+AGG | + | chr7.4:18888623-18888642 | None:intergenic | 25.0% |
| !!! | CATGCTATTTTTTGTAAACT+TGG | - | chr7.4:18888488-18888507 | MS.gene38763:intron | 25.0% |
| AAAGAGATGATAAAGGAATC+AGG | - | chr7.4:18888769-18888788 | MS.gene38763:CDS | 30.0% | |
| ACACCATAAAGGAAAAAACA+AGG | - | chr7.4:18888714-18888733 | MS.gene38763:CDS | 30.0% | |
| AGGGAGCTTAATAAAAAGTT+TGG | - | chr7.4:18888234-18888253 | MS.gene38763:intron | 30.0% | |
| CCTGGAATGAAAAATTCATT+TGG | + | chr7.4:18888892-18888911 | None:intergenic | 30.0% | |
| TAACTGAGAAAAAGTTTCCT+AGG | + | chr7.4:18888192-18888211 | None:intergenic | 30.0% | |
| TAGTGATGTTGTAACAATTG+TGG | - | chr7.4:18888416-18888435 | MS.gene38763:intron | 30.0% | |
| TTTAACTGAAGAGAACTTCA+AGG | - | chr7.4:18888576-18888595 | MS.gene38763:intron | 30.0% | |
| ! | AATTCATTTGGAATTGCTTG+AGG | + | chr7.4:18888880-18888899 | None:intergenic | 30.0% |
| ! | CCAAATGAATTTTTCATTCC+AGG | - | chr7.4:18888889-18888908 | MS.gene38763:intron | 30.0% |
| ! | TCTTTGAAGAAATTAACACC+AGG | - | chr7.4:18887860-18887879 | MS.gene38763:CDS | 30.0% |
| ATAAAGGAATCAGGATTCAC+TGG | - | chr7.4:18888778-18888797 | MS.gene38763:CDS | 35.0% | |
| ATTGACAAAAGCAGAATTCC+AGG | - | chr7.4:18888738-18888757 | MS.gene38763:CDS | 35.0% | |
| GATGTTGTAACAATTGTGGT+CGG | - | chr7.4:18888420-18888439 | MS.gene38763:intron | 35.0% | |
| GTATTGTAGGAACACCATAA+AGG | - | chr7.4:18888703-18888722 | MS.gene38763:intron | 35.0% | |
| TATAATTGGTCCAAGTTCCT+TGG | + | chr7.4:18888007-18888026 | None:intergenic | 35.0% | |
| TCATCTCTTTCATAATCTCC+TGG | + | chr7.4:18888759-18888778 | None:intergenic | 35.0% | |
| TCTAATAAGACAGTCACCTT+AGG | - | chr7.4:18888604-18888623 | MS.gene38763:intron | 35.0% | |
| TGAGAGTTAGATATAGAACC+TGG | + | chr7.4:18887881-18887900 | None:intergenic | 35.0% | |
| TGGCTATATACAACTAAACG+AGG | - | chr7.4:18888508-18888527 | MS.gene38763:CDS | 35.0% | |
| ! | ACTTTTCATCATTCTATGGC+AGG | - | chr7.4:18888214-18888233 | MS.gene38763:intron | 35.0% |
| ! | CTGTTTGTTAAGCAAAGAGT+GGG | - | chr7.4:18888853-18888872 | MS.gene38763:intron | 35.0% |
| ! | CTTTTCATCATTCTATGGCA+GGG | - | chr7.4:18888215-18888234 | MS.gene38763:intron | 35.0% |
| ! | GAAGCACTCTTATACATCTT+CGG | - | chr7.4:18888814-18888833 | MS.gene38763:intron | 35.0% |
| ! | TCTGTTTGTTAAGCAAAGAG+TGG | - | chr7.4:18888852-18888871 | MS.gene38763:intron | 35.0% |
| ! | TGTTTGTTAAGCAAAGAGTG+GGG | - | chr7.4:18888854-18888873 | MS.gene38763:intron | 35.0% |
| AACTGAAGAGAACTTCAAGG+AGG | - | chr7.4:18888579-18888598 | MS.gene38763:intron | 40.0% | |
| AACTGATGAAAAGAAGGCCA+AGG | - | chr7.4:18887987-18888006 | MS.gene38763:CDS | 40.0% | |
| TACAACTAAACGAGGCGAAA+TGG | - | chr7.4:18888516-18888535 | MS.gene38763:CDS | 40.0% | |
| TGCAGGAACTGATGAAAAGA+AGG | - | chr7.4:18887981-18888000 | MS.gene38763:CDS | 40.0% | |
| TTAACAAACAGAGCAGTCAC+AGG | + | chr7.4:18888844-18888863 | None:intergenic | 40.0% | |
| CATGGTGTGTGTGCAATCAA+TGG | - | chr7.4:18888656-18888675 | MS.gene38763:intron | 45.0% | |
| CTACTCCTCGGTAGAATTCT+GGG | + | chr7.4:18888074-18888093 | None:intergenic | 45.0% | |
| GAAAAGAAGGCCAAGGAACT+TGG | - | chr7.4:18887994-18888013 | MS.gene38763:CDS | 45.0% | |
| TCAGATCAAACTCCCCAAAG+CGG | - | chr7.4:18888263-18888282 | MS.gene38763:intron | 45.0% | |
| TGAATTCGCGACCACAATCT+CGG | - | chr7.4:18888460-18888479 | MS.gene38763:intron | 45.0% | |
| TTCTCAAGTTCCTCCGCTTT+GGG | + | chr7.4:18888279-18888298 | None:intergenic | 45.0% | |
| ACTGTGACTGAAGTGATCCC+TGG | + | chr7.4:18888910-18888929 | None:intergenic | 50.0% | |
| AGCATGCAAACCCGAGATTG+TGG | + | chr7.4:18888474-18888493 | None:intergenic | 50.0% | |
| CAAAGCGGAGGAACTTGAGA+AGG | - | chr7.4:18888278-18888297 | MS.gene38763:intron | 50.0% | |
| CCAGAATTCTACCGAGGAGT+AGG | - | chr7.4:18888072-18888091 | MS.gene38763:CDS | 50.0% | |
| CCTACTCCTCGGTAGAATTC+TGG | + | chr7.4:18888075-18888094 | None:intergenic | 50.0% | |
| CTTCTCAAGTTCCTCCGCTT+TGG | + | chr7.4:18888280-18888299 | None:intergenic | 50.0% | |
| GAATCAGGATTCACTGGCGT+GGG | - | chr7.4:18888784-18888803 | MS.gene38763:CDS | 50.0% | |
| GAATTCGCGACCACAATCTC+GGG | - | chr7.4:18888461-18888480 | MS.gene38763:intron | 50.0% | |
| GACTTCCCAGAATTCTACCG+AGG | - | chr7.4:18888066-18888085 | MS.gene38763:CDS | 50.0% | |
| TCTCAAGTTCCTCCGCTTTG+GGG | + | chr7.4:18888278-18888297 | None:intergenic | 50.0% | |
| ATTCACTGGCGTGGGAGCAA+AGG | - | chr7.4:18888792-18888811 | MS.gene38763:CDS | 55.0% | |
| CGACTATCTCGCCTACTCCT+CGG | + | chr7.4:18888086-18888105 | None:intergenic | 55.0% | |
| GATCAAACTCCCCAAAGCGG+AGG | - | chr7.4:18888266-18888285 | MS.gene38763:intron | 55.0% | |
| GGAATCAGGATTCACTGGCG+TGG | - | chr7.4:18888783-18888802 | MS.gene38763:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 18887851 | 18888960 | 18887851 | ID=MS.gene38763 |
| chr7.4 | mRNA | 18887851 | 18888960 | 18887851 | ID=MS.gene38763.t1;Parent=MS.gene38763 |
| chr7.4 | exon | 18888930 | 18888960 | 18888930 | ID=MS.gene38763.t1.exon1;Parent=MS.gene38763.t1 |
| chr7.4 | CDS | 18888930 | 18888960 | 18888930 | ID=cds.MS.gene38763.t1;Parent=MS.gene38763.t1 |
| chr7.4 | exon | 18888705 | 18888825 | 18888705 | ID=MS.gene38763.t1.exon2;Parent=MS.gene38763.t1 |
| chr7.4 | CDS | 18888705 | 18888825 | 18888705 | ID=cds.MS.gene38763.t1;Parent=MS.gene38763.t1 |
| chr7.4 | exon | 18888503 | 18888575 | 18888503 | ID=MS.gene38763.t1.exon3;Parent=MS.gene38763.t1 |
| chr7.4 | CDS | 18888503 | 18888575 | 18888503 | ID=cds.MS.gene38763.t1;Parent=MS.gene38763.t1 |
| chr7.4 | exon | 18887851 | 18888099 | 18887851 | ID=MS.gene38763.t1.exon4;Parent=MS.gene38763.t1 |
| chr7.4 | CDS | 18887851 | 18888099 | 18887851 | ID=cds.MS.gene38763.t1;Parent=MS.gene38763.t1 |
| Gene Sequence |
| Protein sequence |
