Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42982.t1 | RHN40757.1 | 83.3 | 156 | 24 | 2 | 1 | 156 | 157 | 310 | 1.10E-45 | 193 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42982.t1 | Q9LDD1 | 46.9 | 160 | 62 | 5 | 1 | 155 | 192 | 333 | 1.0e-28 | 127.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42982.t1 | A0A396GPW6 | 83.3 | 156 | 24 | 2 | 1 | 156 | 157 | 310 | 7.6e-46 | 193.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene42982 | 0.828149 | 1.06E-54 | -1.69E-46 |
| MS.gene04918 | MS.gene42982 | 0.804649 | 1.95E-49 | -1.69E-46 |
| MS.gene049350 | MS.gene42982 | 0.823218 | 1.56E-53 | -1.69E-46 |
| MS.gene049368 | MS.gene42982 | 0.824802 | 6.63E-54 | -1.69E-46 |
| MS.gene049711 | MS.gene42982 | 0.800337 | 1.51E-48 | -1.69E-46 |
| MS.gene051279 | MS.gene42982 | 0.805693 | 1.18E-49 | -1.69E-46 |
| MS.gene051700 | MS.gene42982 | 0.803275 | 3.77E-49 | -1.69E-46 |
| MS.gene051852 | MS.gene42982 | 0.816176 | 6.33E-52 | -1.69E-46 |
| MS.gene052307 | MS.gene42982 | 0.80472 | 1.89E-49 | -1.69E-46 |
| MS.gene052463 | MS.gene42982 | 0.835904 | 1.28E-56 | -1.69E-46 |
| MS.gene052516 | MS.gene42982 | 0.809394 | 1.93E-50 | -1.69E-46 |
| MS.gene052518 | MS.gene42982 | 0.809089 | 2.25E-50 | -1.69E-46 |
| MS.gene053226 | MS.gene42982 | 0.805371 | 1.38E-49 | -1.69E-46 |
| MS.gene05458 | MS.gene42982 | 0.812246 | 4.67E-51 | -1.69E-46 |
| MS.gene054651 | MS.gene42982 | 0.809263 | 2.06E-50 | -1.69E-46 |
| MS.gene055468 | MS.gene42982 | 0.813259 | 2.80E-51 | -1.69E-46 |
| MS.gene055513 | MS.gene42982 | 0.815306 | 9.89E-52 | -1.69E-46 |
| MS.gene056308 | MS.gene42982 | 0.808926 | 2.44E-50 | -1.69E-46 |
| MS.gene057102 | MS.gene42982 | 0.814674 | 1.37E-51 | -1.69E-46 |
| MS.gene059170 | MS.gene42982 | 0.816551 | 5.22E-52 | -1.69E-46 |
| MS.gene060435 | MS.gene42982 | 0.803983 | 2.69E-49 | -1.69E-46 |
| MS.gene060763 | MS.gene42982 | 0.801848 | 7.42E-49 | -1.69E-46 |
| MS.gene060935 | MS.gene42982 | 0.802724 | 4.90E-49 | -1.69E-46 |
| MS.gene060937 | MS.gene42982 | 0.806587 | 7.65E-50 | -1.69E-46 |
| MS.gene061100 | MS.gene42982 | 0.822474 | 2.32E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42982.t1 | MTR_2g069350 | 48.256 | 172 | 66 | 5 | 1 | 155 | 200 | 365 | 4.71e-43 | 146 |
| MS.gene42982.t1 | MTR_2g028830 | 44.025 | 159 | 59 | 4 | 1 | 155 | 186 | 318 | 1.84e-34 | 122 |
| MS.gene42982.t1 | MTR_4g083540 | 40.260 | 154 | 64 | 3 | 2 | 155 | 167 | 292 | 4.01e-29 | 108 |
| MS.gene42982.t1 | MTR_5g013690 | 32.484 | 157 | 78 | 3 | 2 | 155 | 140 | 271 | 8.95e-16 | 72.4 |
| MS.gene42982.t1 | MTR_5g013690 | 33.758 | 157 | 76 | 4 | 2 | 155 | 203 | 334 | 1.34e-15 | 72.8 |
| MS.gene42982.t1 | MTR_1g019250 | 29.870 | 154 | 85 | 5 | 2 | 155 | 210 | 340 | 2.30e-11 | 60.8 |
| MS.gene42982.t1 | MTR_4g036485 | 30.201 | 149 | 64 | 5 | 5 | 151 | 97 | 207 | 8.90e-11 | 58.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42982.t1 | AT3G12920 | 48.750 | 160 | 59 | 5 | 1 | 155 | 192 | 333 | 5.55e-40 | 137 |
| MS.gene42982.t1 | AT1G79110 | 43.871 | 155 | 79 | 2 | 1 | 155 | 185 | 331 | 4.73e-33 | 119 |
| MS.gene42982.t1 | AT1G79110 | 43.871 | 155 | 79 | 2 | 1 | 155 | 207 | 353 | 5.77e-33 | 119 |
| MS.gene42982.t1 | AT1G79110 | 43.871 | 155 | 79 | 2 | 1 | 155 | 210 | 356 | 6.53e-33 | 119 |
| MS.gene42982.t1 | AT5G45100 | 41.401 | 157 | 62 | 5 | 1 | 155 | 137 | 265 | 8.40e-30 | 109 |
| MS.gene42982.t1 | AT5G45100 | 41.401 | 157 | 62 | 5 | 1 | 155 | 164 | 292 | 1.47e-29 | 109 |
| MS.gene42982.t1 | AT4G19700 | 39.490 | 157 | 65 | 5 | 1 | 155 | 174 | 302 | 3.87e-27 | 103 |
| MS.gene42982.t1 | AT1G10650 | 32.468 | 154 | 80 | 5 | 2 | 153 | 149 | 280 | 2.00e-13 | 66.2 |
| MS.gene42982.t1 | AT1G60610 | 30.872 | 149 | 85 | 4 | 5 | 153 | 207 | 337 | 4.09e-13 | 65.9 |
| MS.gene42982.t1 | AT1G60610 | 30.872 | 149 | 85 | 4 | 5 | 153 | 207 | 337 | 4.09e-13 | 65.9 |
| MS.gene42982.t1 | AT1G60610 | 30.872 | 149 | 85 | 4 | 5 | 153 | 207 | 337 | 4.09e-13 | 65.9 |
| MS.gene42982.t1 | AT1G60610 | 30.872 | 149 | 85 | 4 | 5 | 153 | 207 | 337 | 4.09e-13 | 65.9 |
| MS.gene42982.t1 | AT1G60610 | 30.872 | 149 | 85 | 4 | 5 | 153 | 207 | 337 | 4.09e-13 | 65.9 |
| MS.gene42982.t1 | AT1G10650 | 32.450 | 151 | 78 | 5 | 5 | 153 | 208 | 336 | 4.82e-13 | 65.5 |
| MS.gene42982.t1 | AT4G35070 | 29.412 | 153 | 80 | 4 | 2 | 153 | 82 | 207 | 1.98e-12 | 62.8 |
| MS.gene42982.t1 | AT4G35070 | 31.126 | 151 | 76 | 5 | 2 | 151 | 137 | 260 | 9.12e-12 | 61.6 |
Find 27 sgRNAs with CRISPR-Local
Find 34 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTGTTCAATGTGTGGGGCTA+TGG | 0.402214 | 8.1:+54775524 | MS.gene42982:CDS |
| AGTAATGACGGCGCAGGATA+AGG | 0.404721 | 8.1:+54775395 | MS.gene42982:CDS |
| ACAAATCCTTATGCAACAAA+TGG | 0.418067 | 8.1:+54775251 | MS.gene42982:CDS |
| GATTTAACAGAATCACAAAT+AGG | 0.421677 | 8.1:-54775558 | None:intergenic |
| ATATTCATCATTACTACAAC+AGG | 0.424115 | 8.1:-54775355 | None:intergenic |
| TATGCATGGAGAATCAAATC+TGG | 0.461576 | 8.1:+54775172 | MS.gene42982:CDS |
| TATGTTTGTGTTCAATGTGT+GGG | 0.464104 | 8.1:+54775517 | MS.gene42982:CDS |
| CACAAACATAAGTGTCTACA+AGG | 0.505076 | 8.1:-54775504 | None:intergenic |
| AAGGATGAAGGTACTAGTAG+TGG | 0.520250 | 8.1:+54775414 | MS.gene42982:CDS |
| AAAAGTGAAAACACTATGCA+TGG | 0.524997 | 8.1:+54775158 | MS.gene42982:CDS |
| TGTAGTAATGATGAATATTG+TGG | 0.529768 | 8.1:+54775360 | MS.gene42982:CDS |
| ATAGATTATGCAGAAACTGT+GGG | 0.549558 | 8.1:+54775454 | MS.gene42982:CDS |
| TTATGTTTGTGTTCAATGTG+TGG | 0.554213 | 8.1:+54775516 | MS.gene42982:CDS |
| GACGGTGAATATAGCGGTGG+CGG | 0.555960 | 8.1:+54775297 | MS.gene42982:CDS |
| AGCATCCTCCGCCACCGCAA+CGG | 0.571002 | 8.1:-54775328 | None:intergenic |
| GTAGTAATGATGAATATTGT+GGG | 0.574573 | 8.1:+54775361 | MS.gene42982:CDS |
| AATAGATTATGCAGAAACTG+TGG | 0.580193 | 8.1:+54775453 | MS.gene42982:CDS |
| TTATGCAGAAACTGTGGGGA+AGG | 0.589265 | 8.1:+54775459 | MS.gene42982:CDS |
| ATGTTTGTGTTCAATGTGTG+GGG | 0.595264 | 8.1:+54775518 | MS.gene42982:CDS |
| ACGGCGCAGGATAAGGATGA+AGG | 0.596413 | 8.1:+54775402 | MS.gene42982:CDS |
| TGAATATTGTGGGTGGCGTA+CGG | 0.612792 | 8.1:+54775371 | MS.gene42982:CDS |
| TACGGTAGTAATGACGGCGC+AGG | 0.633033 | 8.1:+54775389 | MS.gene42982:CDS |
| GTGGCGTACGGTAGTAATGA+CGG | 0.665628 | 8.1:+54775383 | MS.gene42982:CDS |
| GGCGACGGTGAATATAGCGG+TGG | 0.684894 | 8.1:+54775294 | MS.gene42982:CDS |
| TAGATTATGCAGAAACTGTG+GGG | 0.699966 | 8.1:+54775455 | MS.gene42982:CDS |
| AACGGCGACGGTGAATATAG+CGG | 0.708230 | 8.1:+54775291 | MS.gene42982:CDS |
| GTAATGATGAATATTGTGGG+TGG | 0.732674 | 8.1:+54775364 | MS.gene42982:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATATTCATCATTACTACAAC+AGG | - | chr8.1:54775358-54775377 | None:intergenic | 25.0% |
| ! | GAAAACAACAACAAAAACAA+CGG | + | chr8.1:54775273-54775292 | MS.gene42982:CDS | 25.0% |
| ! | GATTTAACAGAATCACAAAT+AGG | - | chr8.1:54775561-54775580 | None:intergenic | 25.0% |
| ! | GTAGTAATGATGAATATTGT+GGG | + | chr8.1:54775361-54775380 | MS.gene42982:CDS | 25.0% |
| ! | TGTAGTAATGATGAATATTG+TGG | + | chr8.1:54775360-54775379 | MS.gene42982:CDS | 25.0% |
| AAAAGTGAAAACACTATGCA+TGG | + | chr8.1:54775158-54775177 | MS.gene42982:CDS | 30.0% | |
| AATAGATTATGCAGAAACTG+TGG | + | chr8.1:54775453-54775472 | MS.gene42982:CDS | 30.0% | |
| ACAAATCCTTATGCAACAAA+TGG | + | chr8.1:54775251-54775270 | MS.gene42982:CDS | 30.0% | |
| ATAGATTATGCAGAAACTGT+GGG | + | chr8.1:54775454-54775473 | MS.gene42982:CDS | 30.0% | |
| TATGTTTGTGTTCAATGTGT+GGG | + | chr8.1:54775517-54775536 | MS.gene42982:CDS | 30.0% | |
| TTATGTTTGTGTTCAATGTG+TGG | + | chr8.1:54775516-54775535 | MS.gene42982:CDS | 30.0% | |
| !! | TGTTTTCCATTTGTTGCATA+AGG | - | chr8.1:54775260-54775279 | None:intergenic | 30.0% |
| ATGTTTGTGTTCAATGTGTG+GGG | + | chr8.1:54775518-54775537 | MS.gene42982:CDS | 35.0% | |
| CACAAACATAAGTGTCTACA+AGG | - | chr8.1:54775507-54775526 | None:intergenic | 35.0% | |
| GTAATGATGAATATTGTGGG+TGG | + | chr8.1:54775364-54775383 | MS.gene42982:CDS | 35.0% | |
| TAGATTATGCAGAAACTGTG+GGG | + | chr8.1:54775455-54775474 | MS.gene42982:CDS | 35.0% | |
| TATGCATGGAGAATCAAATC+TGG | + | chr8.1:54775172-54775191 | MS.gene42982:CDS | 35.0% | |
| AACAACAAAAACAACGGCGA+CGG | + | chr8.1:54775279-54775298 | MS.gene42982:CDS | 40.0% | |
| !! | AAGGATGAAGGTACTAGTAG+TGG | + | chr8.1:54775414-54775433 | MS.gene42982:CDS | 40.0% |
| TGAATATTGTGGGTGGCGTA+CGG | + | chr8.1:54775371-54775390 | MS.gene42982:CDS | 45.0% | |
| TTATGCAGAAACTGTGGGGA+AGG | + | chr8.1:54775459-54775478 | MS.gene42982:CDS | 45.0% | |
| AACGGCGACGGTGAATATAG+CGG | + | chr8.1:54775291-54775310 | MS.gene42982:CDS | 50.0% | |
| AGTAATGACGGCGCAGGATA+AGG | + | chr8.1:54775395-54775414 | MS.gene42982:CDS | 50.0% | |
| GTGGCGTACGGTAGTAATGA+CGG | + | chr8.1:54775383-54775402 | MS.gene42982:CDS | 50.0% | |
| GTGTTCAATGTGTGGGGCTA+TGG | + | chr8.1:54775524-54775543 | MS.gene42982:CDS | 50.0% | |
| ACGGCGCAGGATAAGGATGA+AGG | + | chr8.1:54775402-54775421 | MS.gene42982:CDS | 55.0% | |
| GACGGTGAATATAGCGGTGG+CGG | + | chr8.1:54775297-54775316 | MS.gene42982:CDS | 55.0% | |
| TACGGTAGTAATGACGGCGC+AGG | + | chr8.1:54775389-54775408 | MS.gene42982:CDS | 55.0% | |
| ! | AGATTTTGCCGTTGCGGTGG+CGG | + | chr8.1:54775320-54775339 | MS.gene42982:CDS | 55.0% |
| ! | TGGCGGAGATTTTGCCGTTG+CGG | + | chr8.1:54775314-54775333 | MS.gene42982:CDS | 55.0% |
| GGCGACGGTGAATATAGCGG+TGG | + | chr8.1:54775294-54775313 | MS.gene42982:CDS | 60.0% | |
| ! | CGGAGATTTTGCCGTTGCGG+TGG | + | chr8.1:54775317-54775336 | MS.gene42982:CDS | 60.0% |
| AGCATCCTCCGCCACCGCAA+CGG | - | chr8.1:54775331-54775350 | None:intergenic | 65.0% | |
| !! | TTTTGCCGTTGCGGTGGCGG+AGG | + | chr8.1:54775323-54775342 | MS.gene42982:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 54775138 | 54775608 | 54775138 | ID=MS.gene42982 |
| chr8.1 | mRNA | 54775138 | 54775608 | 54775138 | ID=MS.gene42982.t1;Parent=MS.gene42982 |
| chr8.1 | exon | 54775138 | 54775608 | 54775138 | ID=MS.gene42982.t1.exon1;Parent=MS.gene42982.t1 |
| chr8.1 | CDS | 54775138 | 54775608 | 54775138 | ID=cds.MS.gene42982.t1;Parent=MS.gene42982.t1 |
| Gene Sequence |
| Protein sequence |
