AlfalfaGEDB | MS.gene43800

M. sativa cultivar XinJiangDaYe / MS.gene43800

KEGG: K07300
GO: GO:0003674 GO:0005215 GO:0005451 GO:0005575 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0008150 GO:0008324 GO:0015075 GO:0015077 GO:0015078 GO:0015085 GO:0015291 GO:0015297 GO:0015298 GO:0015299 GO:0015318 GO:0015368 GO:0015369 GO:0015491 GO:0016021 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0046873 GO:0051139 GO:0051179 GO:0051234 GO:0055085 GO:0070838 GO:0072509 GO:0072511 GO:0110165
PFAM: PF01699.27

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene43800.t1	XP_003615635.1	99.1	435	3	1	1	434	1	435	2.30E-231	811.2

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene43800.t1	Q93Z81	69.3	417	117	5	26	433	33	447	1.6e-153	543.9

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene43800.t1	G7KE49	99.1	435	3	1	1	434	1	435	1.7e-231	811.2

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene053074	MS.gene43800	0.832371	9.82E-56	-1.69E-46
MS.gene053184	MS.gene43800	0.80898	2.37E-50	-1.69E-46
MS.gene054184	MS.gene43800	0.816021	6.85E-52	-1.69E-46
MS.gene054185	MS.gene43800	0.82024	7.62E-53	-1.69E-46
MS.gene054713	MS.gene43800	0.846041	2.79E-59	-1.69E-46
MS.gene055860	MS.gene43800	0.819463	1.15E-52	-1.69E-46
MS.gene057754	MS.gene43800	0.802257	6.11E-49	-1.69E-46
MS.gene058042	MS.gene43800	0.856691	2.71E-62	-1.69E-46
MS.gene058168	MS.gene43800	0.805165	1.52E-49	-1.69E-46
MS.gene059006	MS.gene43800	0.803459	3.45E-49	-1.69E-46

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PPI:

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene43800.t1	MTR_5g070330	99.080	435	3	1	1	434	1	435	0.0	873
MS.gene43800.t1	MTR_4g016720	69.880	415	111	6	25	432	34	441	0.0	546
MS.gene43800.t1	MTR_7g068380	70.123	405	114	4	25	425	34	435	0.0	512
MS.gene43800.t1	MTR_2g105640	46.455	409	201	6	27	425	38	438	2.56e-114	342
MS.gene43800.t1	MTR_3g082180	45.169	414	211	6	18	419	15	424	1.29e-110	333
MS.gene43800.t1	MTR_8g033030	64.198	81	29	0	108	188	16	96	5.77e-26	101

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene43800.t1	AT2G38170	67.482	409	122	5	25	425	32	437	0.0	536
MS.gene43800.t1	AT2G38170	67.482	409	122	5	25	425	32	437	0.0	536
MS.gene43800.t1	AT3G51860	68.571	420	117	5	25	433	32	447	0.0	535
MS.gene43800.t1	AT5G01490	58.333	408	157	3	28	424	35	440	6.55e-176	499
MS.gene43800.t1	AT5G01490	58.333	408	157	3	28	424	35	440	6.55e-176	499
MS.gene43800.t1	AT3G51860	69.110	382	103	5	25	395	32	409	1.07e-168	482
MS.gene43800.t1	AT2G38170	66.149	322	98	5	25	338	32	350	3.47e-138	401
MS.gene43800.t1	AT1G55730	51.181	381	171	3	55	425	62	437	5.18e-123	365
MS.gene43800.t1	AT1G55730	51.181	381	171	3	55	425	62	437	5.18e-123	365
MS.gene43800.t1	AT3G13320	49.606	381	177	3	55	425	62	437	5.93e-120	357

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Find 77 sgRNAs with CRISPR-Local

Find 315 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGAAATCAGATATGGTTT+TGG	0.357259	5.1:-54658391	MS.gene43800:CDS
AAATATGAAAGAGGTTATGT+TGG	0.461852	5.1:-54658325	MS.gene43800:CDS
AAATCAGCAGCAACAGCTAA+AGG	0.486221	5.1:+54658270	None:intergenic
AAGCTGACACGTTCAGCTAG+GGG	0.628561	5.1:+54656139	None:intergenic
AAGGCATACTTGAACATCAA+TGG	0.533262	5.1:+54655000	None:intergenic
AATAATCGTACCTGTTGGGC+CGG	0.563639	5.1:+54655942	None:intergenic
AATATGAAAGAGGTTATGTT+GGG	0.517574	5.1:-54658324	MS.gene43800:CDS
AATGGGTATAAGAATGGATT+TGG	0.331250	5.1:-54653615	MS.gene43800:CDS
ACATGCAAACTTACTCAGTG+AGG	0.690735	5.1:+54656116	None:intergenic
ACGACGATAGCTTCAAAGCA+TGG	0.608256	5.1:+54652265	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AATTTCTTAACTGTGAATGC+AGG	-	chr5.1:54656842-54656861	MS.gene43800:intron	30.0%
ATATAAAGTTGTAGCTCAAG+TGG	-	chr5.1:54654029-54654048	MS.gene43800:intron	30.0%
ATTACAAAATAGTCCCTGAA+TGG	+	chr5.1:54653548-54653567	None:intergenic	30.0%
ATTTAATCCACTTATGTGTG+AGG	-	chr5.1:54656366-54656385	MS.gene43800:intron	30.0%
ATTTGCTTACAAGAACAAGT+TGG	-	chr5.1:54656737-54656756	MS.gene43800:intron	30.0%
CATCTCCTATAAATAGGATT+AGG	+	chr5.1:54658042-54658061	None:intergenic	30.0%
GATTAACATAGTGTACATTC+AGG	-	chr5.1:54653467-54653486	MS.gene43800:intron	30.0%
GATTATTGCTCTTTCATGTA+TGG	-	chr5.1:54654852-54654871	MS.gene43800:intron	30.0%
GCTCAGTTTATTGAAATTTC+TGG	+	chr5.1:54655209-54655228	None:intergenic	30.0%
GTGTTCCTAATCCTATTTAT+AGG	-	chr5.1:54658034-54658053	MS.gene43800:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5.1	gene	54652277	54658523	54652277	ID=MS.gene43800
chr5.1	mRNA	54652277	54658523	54652277	ID=MS.gene43800.t1;Parent=MS.gene43800
chr5.1	exon	54658254	54658523	54658254	ID=MS.gene43800.t1.exon1;Parent=MS.gene43800.t1
chr5.1	CDS	54658254	54658523	54658254	ID=cds.MS.gene43800.t1;Parent=MS.gene43800.t1
chr5.1	exon	54656130	54656197	54656130	ID=MS.gene43800.t1.exon2;Parent=MS.gene43800.t1
chr5.1	CDS	54656130	54656197	54656130	ID=cds.MS.gene43800.t1;Parent=MS.gene43800.t1
chr5.1	exon	54655953	54655981	54655953	ID=MS.gene43800.t1.exon3;Parent=MS.gene43800.t1
chr5.1	CDS	54655953	54655981	54655953	ID=cds.MS.gene43800.t1;Parent=MS.gene43800.t1
chr5.1	exon	54655345	54655541	54655345	ID=MS.gene43800.t1.exon4;Parent=MS.gene43800.t1
chr5.1	CDS	54655345	54655541	54655345	ID=cds.MS.gene43800.t1;Parent=MS.gene43800.t1
chr5.1	exon	54654864	54655079	54654864	ID=MS.gene43800.t1.exon5;Parent=MS.gene43800.t1
chr5.1	CDS	54654864	54655079	54654864	ID=cds.MS.gene43800.t1;Parent=MS.gene43800.t1
chr5.1	exon	54654283	54654399	54654283	ID=MS.gene43800.t1.exon6;Parent=MS.gene43800.t1
chr5.1	CDS	54654283	54654399	54654283	ID=cds.MS.gene43800.t1;Parent=MS.gene43800.t1
chr5.1	exon	54654042	54654158	54654042	ID=MS.gene43800.t1.exon7;Parent=MS.gene43800.t1
chr5.1	CDS	54654042	54654158	54654042	ID=cds.MS.gene43800.t1;Parent=MS.gene43800.t1
chr5.1	exon	54653883	54653936	54653883	ID=MS.gene43800.t1.exon8;Parent=MS.gene43800.t1
chr5.1	CDS	54653883	54653936	54653883	ID=cds.MS.gene43800.t1;Parent=MS.gene43800.t1
chr5.1	exon	54653550	54653666	54653550	ID=MS.gene43800.t1.exon9;Parent=MS.gene43800.t1
chr5.1	CDS	54653550	54653666	54653550	ID=cds.MS.gene43800.t1;Parent=MS.gene43800.t1
chr5.1	exon	54653357	54653450	54653357	ID=MS.gene43800.t1.exon10;Parent=MS.gene43800.t1
chr5.1	CDS	54653357	54653450	54653357	ID=cds.MS.gene43800.t1;Parent=MS.gene43800.t1
chr5.1	exon	54652277	54652302	54652277	ID=MS.gene43800.t1.exon11;Parent=MS.gene43800.t1
chr5.1	CDS	54652277	54652302	54652277	ID=cds.MS.gene43800.t1;Parent=MS.gene43800.t1

Gene Sequence

>MS.gene43800

ATGAGCTTCTTCTCAGAGTCTACATCACGACCATTTCGAACCATGAATGAAGATTTGGAGAACAATAATGTAGAGACACCACATAACATTTCATCAAATTCTATTGTGAGAAAGAAATCAGATATGGTTTTGGTGACAAATAATGTTCGGTTCCAAATACTAAGAAATGTGATGACAAATATGAAAGAGGTTATGTTGGGTACTAAGCTTGTTGTGCTTTTTCCGGCTGTTCCTTTAGCTGTTGCTGCTGATTTTTATAGCCTTGGAAGGGTAAGTGTAATTAGCACCCATTAATTCATATTATGACTTCTAATTAGTTTTTTAAACTATAATATTAGCATTTGTTAGTTTTTTAAACTGAGGATTGTGATCATATTTATCACTTCATTCCTAACTTCACCACCTCAGTAATCACAAAACCATCTTTATATGCCATGACTTCTAATCTAATAAGTTACATTACACATTGCATGTATGCACATGTGTAAGTATATGTGAACTCAACTTAATCGTTATTAGTTGTTTCTTGAAATGTGTTATTAGTTATAAATATTTTTTCAAACAACGTTTAATGTTTCTATTTCACAAGAAATAGATTTCAAGTTAGAACTCAGAACATTATATCCCTCAAAAAAATACATCGCATAATCATCCAAATGGATAAATTATCTATAAATCATCAATTAATTTAAAAAATTAAATAATTTCTAAAGTTGAGTTTCAAGCTTTTGACGAGCTTTCTTGCAATTTTATGGCTATAGATTATTCCATATCATCATATTGTATGTTCACGTTATTTAAAATATATATTATCAATATCTATTAGTTCATAAATATGTGGACGGGACCACAAAACTGATGAAATTTAAAATATGGGAACCATTTCTTTGAGCAGTAAAAATAGATGTAATTGTGCTTAAAAAGATAGATTAGAATATACATACCCTTATACAAGGGTAGCACTTGGGTGACACTCTGGTTGGTGACATTGGCCAACCACGTCACAAGTGGCTTTCTTCTCACAAAAAGTTTCGTCATTTTTCTTAACTAAATAATCCAATTTTTTAAAGGGAATTAGTCACTTTAGTCCCTGAATAAGAAAGGAGTAGTCACTTTAGTCCTTCAATGCAGAGAAATTGCAAAACAGTCCCTGAATATAGACCATGTTGGTCAATTTCAGGGACTAAAGTGATTAACATAGTGTACATTCAGGGACTAAATTGACTAATAGAGTCTGCATTCAGGGACTAAATTGACTGACAGAGTCTCCATTCAGGGACTATTTTGTAATTTATCTGCATTCAAGGACTAAAATGACGGCTCGTTTCCTATTCAGGGACTAAAGTGACTAATCTCCTAATTTTTTAATTTTAAAATTTATAGCTTTTTGTATGATGCCATTGCCTAGTATACGTAGTGCCTAAAAAATCTATATCATTATCATTATACATAAGAATTTTTTTCTTTTTTTGGTAAAAAAATAAGAAGAGTGCTTTGATCGCCGTGAGAAAAAGGTAGAAATCGATATGTGTTATAGGATTAAAGTAATTTAAAATGATTTTTTTTAAAATATATTAAATATAAAAAATTGGCGTGCCTTTAAATAATTAATTATTGAATAATATACTAAAAACCTTAGGATTAATTAAAAATCGTAGTTGAGTCTAAAACAACCGGTGGCGGAGCCAGCAGTATTTTTATGGAGGGGCCATAGTTTTACAATATATACATATATTTTCATTAAATACTTATATATAAAGTTGTAGCTCAAGTGGTTTGGATGTTTTTATATTATTTAGGTCCCTTAACTGTGGTTTTAGGTCCCTTAACTGTGGTTCGAAACTCTCATGTCTATTATTCTATTATTTTCGAGGCATTTTAAAAAAAAAATATACAAAATTCAAGTGAAATGCTAAATTCAAGTGAAATGCTAAAATTTATGGATCAAACTCATGACAAATAGCTTCACCACTTAGTTAGATATAAATACTTGAAATCTAAATGTGAATGTTAATATATATAAATTTAATATAACTTTTAGGTAATATTGTGTATACATACCTATTAAAAATGAAAACTTTGGGAGGCCATGTACCTCCCATGTCCCCCTGGCTCTGTCTCTGAACACAATCCTACAAAACCGATTTATATGAGGTAAAGATTATCCACTTATACACATATATTTAGGTCATCTTTTGTTTGATGTGGAACTCTTTAACAAAATTGTCATCGTGTATGAAATAAGAAAATTAATGTGATTTTACCTCAAAAGAAAAAATGATTTAGTATGTTTGTCAAATAATATAATTGTTTTTCTCCATCGTAGCCTTGGATTTTTGCTTTGAGCCTACTTGGACTTGCTCCCCTAGCTGAACGTGTCAGCTTCCTCACTGAGTAAGTTTGCATGTTACATTCTAATTTAATTCATCAGAGTCGGTTACTCACTATACTATGATTAGTTTTCAAAATTCATGCGTTTCTATCATTTGTTATCTAATTTTTTGTTACTGAAAATTTTCACCTGTACTGCATGAACTTGAAGGCAAATTGCATATTTCACCGGCCCAACAGGTACGATTATTGCTCTTTCATGTATGGATTAGACTAGTATACAAATTTGAAATAAATCGAAAAATATCTTATATACCAACATTTGTCTTTATATATTAGTGTAAAATATGGTCAAAGTAAGTTCTATGAATAATCAACTTGATCAAATTGCGATATGTATTTAGAAACAGAAGGAGTAATATTAGTAGACAGTTTTACCTTGTACCCCCAGCTTGAGATCCTACACCCATAAATATATCAAAAACTCCATTTTACCCTTAATCACTTATAATTCTTTTATTTTTCAAATTTTGTAAGTAAATTCAAAATTTGGAAATACTTTATTTTTCTTTTAACAAATTTAAAAATTTTAAGTTTTCCAGAAATTTCAATAAACTGAGCTATCCAAAACAATTTGTGTGAATATTTCAGTTGGAGGGCTTCTAAATGCAACTTGTGGTAATGCAACCGAGATGATCATAGCAATATTAGCACTTCATCAGAACAAAATACATGTTGTCAAGTTCTCCTTGCTTGGTTCCATTCTCTCAAACCTTCTCTTGGTTCTAGGCAGCTCACTCCTTTGTGGTGGCTTAGCAAACCTTAAGAGGGAACAAAGATATGACAGAGTGAGTATTTGATTATTTTTACCTTTTTAATTACATACTGTGCTTACATTTTGGGACGAAGGAAGTACATAAATATTCGATAAGGTTATGATATAATAAACATATTATTCTCTCTTTCAATTATACTTTTTTTTAATTTGTGTGAAATGATCAAATATGTCGATTATTGTGGGACAGATTAAGCATATGTTATTTGATTTTCTTTGTTAATTTAATTTTGTTTAACTAATCAACTTGTTATGTTATTTGATTATTAAATGAACAGAAACAAGCGGATGTAAATTCATTGCTTTTGTTGCTTGGATTGCTATGCCATTTGCTGCCATTGATGTTCAAGTATGCCTTGGCGGGTGGCAATCATTCTATAGCCAATTCTACTCTTCAATTGTCAAGAGCAAGCAGTGTTGTTATGCTTCTAGCATATGTTGCATATATCTTCTTCCAATTGAAAACTCATCGAAAAATATTTGATGCACAAGAGGTTATTACTAATTATGAAATTTCACATTTGAGCACAATTGTATATTGATGTATACCAAAGAATTTAATTAGTGCACATGCATCCAATTATAAGTTGTCATGTCAATTAATAAATATGAAAACACATAAGTTCCCATATTAATTAATTGATGTATCAACACATCATATGATGCATGTGTAAAGTAATTGTACACTGACAGAGTATACTAATTAAACTCTATACTATAATAGAATCGTAAGATAAATGTAGCCACTTAAATTAATTATTATCTTCATACACGGTCAATGTAAAAAGTTTTACGCTATCAGCACATCTCAATATTCAGTCAATCATATATTTGGCGTGGTTATGGTAAAAGACAAACGTTTTTAATTATTTAACAGTTATGATTGATAATGTAAAAACGATTTAGACAAACAATGTATATGAATTTAATCCACTTATGTGTGAGGTTTGATTTTCAGGTGGATGATGAAGATGATGAAAAAGCAGTGATAGGATTTTGGAGTGCATTTAGTTGGTTGGTGGGTATGACATTGGTCATATCTGTGCTTTCTGAGTATGTTGTGGGAACGATTGAGGTAAAGACTACTAATATGTTTATTTTGGTTATAGTGAGCTGAATTTTCAATGAAATTTAAATCAAATACATAATTAGATTAATGGGTTAAGTATCTAACCATTTTATGCATAATAAATAACTAGGCTGCTTCAGATTCTTGGGGTATTTCTGTTAGCTTCATTAGCATAATTTTGCTACCAATTGTTGGGAATGCTGCAGAACATGCTGGTTCAATCATATTTGCTTACAAGAACAAGTTGGTACGGTTTCATCAAAAATATTTTATAGTGGAAATTTCAATTTCTTTACTATATAAGCTTAACTTCATTATATTTGGATTTAAAATTTCTTAACTGTGAATGCAGGACATATCTTTAGGTGTTGCAATGGGATCTGCTACTCAAATTTCTATGTTTGTGGTAAGACTATGTTATTGCAAATTGCAATAATGTACTCTTCTATTGTATGTATATCTTTGATAGTTTGAATTTTACATATATATTCTAAATTTTTTTAATTGATATATATATATGTTTAATTATACTAACTTTGTTTGCATACACAACAATCCTTATTTTATTTTTCAATGGTGGCTTTACACCACTAAGTTTGGATTATTCAATAATCAATTTCAGGTTCCATTAAGTGTGATTGTTGCATGGATAATGGGTATAAGAATGGATTTGGACTTCAATCTTCTTGAAACTGGATGTCTTGGTTTTGCAATTATTGTTACAGCCTTCACTTTACAGGTCTGCACAATGCCTTCATACATTCCACTTTCTCTTTTATTTTATTTTCTTATCTTTTATTTTTCTACTAATTATGTGATTTTGTTAAATCTTTTGTAGGATGGAACTTCACACTATTTGAAAGGACTGATTCTTACTCTATGTTACATTGTCATTTCTGCATGTTTTTTCGTTCTCAAAACTCCACAAATCAGTAAGTATATCTAGCTAGATATCACACATAATAAAACTTCAATTTGTTCATATATTTTAATTTTAATCTCTATTTTTGATGTTGTACCATATGAAAAATAGCACAACTTCAATTTGCCCATATATATATTAATCTCTAATTTTGGGAAATATCAGCAGAAATTTTCTCCTTTGTGAAATAAATTTAAGTACCAAGTGAAAACAAACAAAACAAAGAAAATAAGAAAACAAGTTAAAAGAATCATACAAAAATTAAACTCTTTCATGTGAAAGGGAAAAATTAAGTCTAGTTTAACGAAGTACAACCAGCACAAAAAAGTGAGATTACAATAGCTTTCTTGATATAAGAGAATTCCTCTCAACCTCAGTTGACTCGGATAGCATACAATATCTCCAAACACACACCAAACTATGGTGAAAATGAGACTCATTTTCCCACATAGAGGATTGCTATCACTCATCAATCTCTCCTTGCTCCTGTTGGTTGTCCAAAGAAAAAAGTATTTGTTTTTCTTGGATGAGATTATCCTACTCCTAACATATCTTCCTATTTATAGGAGATGAATATGACTTTTTTTTGTGTGATTACATGTGTTCCTAATCCTATTTATAGGAGATGAATATGGCTTTCTTTGTGATTATAGAAACTTCAAAAATGGACCGTTAATACTTATATCTACAAGAACCATTTTAACTCTCAACATCATACTCTCTATTTCTCAAAAGAAGTGGAGAATTTCATTTCTAAGTTTTTTTTGGGTGTATATAAGATAAGTTATTTTAAAGAATAGTGTCTATAAGCACTTGTACACACAAATTATTAGTTATGAAACTCTTATTTTCTTTCAAAAAATTATAGGGATAGTTGAAAGAAAACATTCTAATGTTTCTTCACAAATAAATTACAAGATCTTGGAACGTTCATCACTTTTTCCATGAACCGTCAATTTTTGTGATTCTTGACTATATATAGTGCACCATCAAACAAAGGAGTGAATTCCAAGTTTTTTTGTTCTTTTTTGTGCTTAATTAAACATTCTTATTTACTGTTGCAGATCATTATGGTATTGCCATGCTTTGA

Protein sequence

>MS.gene43800.t1

MSFFSESTSRPFRTMNEDLENNNVETPHNISSNSIVRKKSDMVLVTNNVRFQILRNVMTNMKEVMLGTKLVVLFPAVPLAVAADFYSLGRPWIFALSLLGLAPLAERVSFLTEQIAYFTGPTVGGLLNATCGNATEMIIAILALHQNKIHVVKFSLLGSILSNLLLVLGSSLLCGGLANLKREQRYDRKQADVNSLLLLLGLLCHLLPLMFKYALAGGNHSIANSTLQLSRASSVVMLLAYVAYIFFQLKTHRKIFDAQEVDDEDDEKAVIGFWSAFSWLVGMTLVISVLSEYVVGTIEAASDSWGISVSFISIILLPIVGNAAEHAGSIIFAYKNKLDISLGVAMGSATQISMFVVPLSVIVAWIMGIRMDLDFNLLETGCLGFAIIVTAFTLQDGTSHYLKGLILTLCYIVISACFFVLKTPQINHYGIAML