Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43995.t1 | XP_013468975.1 | 94.4 | 125 | 7 | 0 | 1 | 125 | 20 | 144 | 1.90E-66 | 261.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43995.t1 | P29063 | 80.8 | 120 | 23 | 0 | 6 | 125 | 26 | 145 | 1.4e-57 | 223.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43995.t1 | A0A072VMG3 | 94.4 | 125 | 7 | 0 | 1 | 125 | 20 | 144 | 1.4e-66 | 261.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049993 | MS.gene43995 | 0.86083 | 1.57E-63 | -1.69E-46 |
| MS.gene050139 | MS.gene43995 | 0.810263 | 1.26E-50 | -1.69E-46 |
| MS.gene050168 | MS.gene43995 | 0.81715 | 3.83E-52 | -1.69E-46 |
| MS.gene051860 | MS.gene43995 | 0.809431 | 1.90E-50 | -1.69E-46 |
| MS.gene051983 | MS.gene43995 | 0.818027 | 2.43E-52 | -1.69E-46 |
| MS.gene052011 | MS.gene43995 | -0.830227 | 3.31E-55 | -1.69E-46 |
| MS.gene052077 | MS.gene43995 | 0.801754 | 7.76E-49 | -1.69E-46 |
| MS.gene054108 | MS.gene43995 | 0.804718 | 1.89E-49 | -1.69E-46 |
| MS.gene054183 | MS.gene43995 | 0.863573 | 2.26E-64 | -1.69E-46 |
| MS.gene055217 | MS.gene43995 | 0.831693 | 1.44E-55 | -1.69E-46 |
| MS.gene055356 | MS.gene43995 | 0.80532 | 1.41E-49 | -1.69E-46 |
| MS.gene055358 | MS.gene43995 | 0.831152 | 1.96E-55 | -1.69E-46 |
| MS.gene055701 | MS.gene43995 | 0.808189 | 3.50E-50 | -1.69E-46 |
| MS.gene056899 | MS.gene43995 | -0.813503 | 2.48E-51 | -1.69E-46 |
| MS.gene056979 | MS.gene43995 | 0.807433 | 5.07E-50 | -1.69E-46 |
| MS.gene057476 | MS.gene43995 | 0.808886 | 2.48E-50 | -1.69E-46 |
| MS.gene059420 | MS.gene43995 | 0.813168 | 2.93E-51 | -1.69E-46 |
| MS.gene059609 | MS.gene43995 | 0.821574 | 3.76E-53 | -1.69E-46 |
| MS.gene061114 | MS.gene43995 | -0.829183 | 5.94E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43995.t1 | MTR_1g080810 | 94.400 | 125 | 7 | 0 | 1 | 125 | 20 | 144 | 1.75e-87 | 251 |
| MS.gene43995.t1 | MTR_1g080820 | 91.200 | 125 | 11 | 0 | 1 | 125 | 20 | 144 | 2.97e-84 | 243 |
| MS.gene43995.t1 | MTR_1g080800 | 86.400 | 125 | 17 | 0 | 1 | 125 | 20 | 144 | 3.50e-78 | 227 |
| MS.gene43995.t1 | MTR_7g115220 | 72.174 | 115 | 32 | 0 | 11 | 125 | 70 | 184 | 1.32e-60 | 185 |
| MS.gene43995.t1 | MTR_1g080780 | 63.200 | 125 | 45 | 1 | 1 | 125 | 16 | 139 | 4.18e-57 | 174 |
| MS.gene43995.t1 | MTR_6g083270 | 49.645 | 141 | 54 | 3 | 2 | 125 | 19 | 159 | 8.97e-43 | 146 |
| MS.gene43995.t1 | MTR_6g083200 | 50.000 | 126 | 59 | 2 | 2 | 125 | 19 | 142 | 3.34e-40 | 133 |
| MS.gene43995.t1 | MTR_6g083310 | 45.714 | 140 | 59 | 4 | 1 | 125 | 18 | 155 | 2.07e-34 | 117 |
| MS.gene43995.t1 | MTR_6g083290 | 43.511 | 131 | 63 | 2 | 2 | 125 | 19 | 145 | 3.58e-33 | 118 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43995.t1 | AT3G04720 | 71.074 | 121 | 35 | 0 | 5 | 125 | 71 | 191 | 4.30e-62 | 189 |
Find 40 sgRNAs with CRISPR-Local
Find 61 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACAACCCTCAGAATATTAAT+TGG | 0.199861 | 1.3:-57154422 | MS.gene43995:CDS |
| CAAGGGCTGGTTTGCATCCC+AGG | 0.360799 | 1.3:+57154368 | None:intergenic |
| CAAAGACATAGTTAACTATA+AGG | 0.374893 | 1.3:+57153651 | None:intergenic |
| TAACTATAAGGTGACCCTTC+TGG | 0.391434 | 1.3:+57153663 | None:intergenic |
| CTCTCTACCACGAGGGCCCT+GGG | 0.401142 | 1.3:+57154305 | None:intergenic |
| CAACCCTCAGAATATTAATT+GGG | 0.408460 | 1.3:-57154421 | MS.gene43995:CDS |
| CAGGTGACAAATACTGCAAC+TGG | 0.420771 | 1.3:-57153785 | MS.gene43995:CDS |
| ACTCTCTACCACGAGGGCCC+TGG | 0.449243 | 1.3:+57154304 | None:intergenic |
| AGGGCCCTGGGGTCCACAAA+AGG | 0.466880 | 1.3:+57154317 | None:intergenic |
| GTCAGAGTGCAAACGGTGTA+AGG | 0.476091 | 1.3:-57154461 | MS.gene43995:CDS |
| CCAGTGTATACTGTGCTACC+TGG | 0.477513 | 1.3:-57154386 | MS.gene43995:CDS |
| TCAAATTGATACCAATGGAC+AGG | 0.479120 | 1.3:-57153690 | MS.gene43995:CDS |
| TAATATTCTGAGGGTTGTAA+AGG | 0.485495 | 1.3:+57154427 | None:intergenic |
| CTGGGATGCAAACCAGCCCT+TGG | 0.489658 | 1.3:-57154367 | MS.gene43995:CDS |
| CGCCAATGGTGGATTAGACT+TGG | 0.495624 | 1.3:-57153729 | MS.gene43995:CDS |
| CTAATCCACCATTGGCGCAC+TGG | 0.495852 | 1.3:+57153735 | None:intergenic |
| GTCTTGTGGCAAGTGCTTGA+GGG | 0.497781 | 1.3:-57154283 | MS.gene43995:intron |
| AGTCTTGTGGCAAGTGCTTG+AGG | 0.518693 | 1.3:-57154284 | MS.gene43995:CDS |
| TAATCCCAATTAATATTCTG+AGG | 0.523387 | 1.3:+57154417 | None:intergenic |
| TTCAATCAAATTGATACCAA+TGG | 0.541175 | 1.3:-57153695 | MS.gene43995:CDS |
| CTTCTGGTAACCCTGTCCAT+TGG | 0.545362 | 1.3:+57153679 | None:intergenic |
| GTGAATGGTCAGAGTGCAAA+CGG | 0.554003 | 1.3:-57154468 | MS.gene43995:CDS |
| TATTCTGAGGGTTGTAAAGG+TGG | 0.557673 | 1.3:+57154430 | None:intergenic |
| ATCCAAGTCTAATCCACCAT+TGG | 0.563000 | 1.3:+57153727 | None:intergenic |
| AATGGACAGGGTTACCAGAA+GGG | 0.563174 | 1.3:-57153677 | MS.gene43995:CDS |
| ATAGTGGACCAGTGCGCCAA+TGG | 0.565572 | 1.3:-57153743 | MS.gene43995:CDS |
| ATGCAAACCAGCCCTTGGAA+TGG | 0.572438 | 1.3:-57154362 | MS.gene43995:CDS |
| AATCCCAATTAATATTCTGA+GGG | 0.576550 | 1.3:+57154418 | None:intergenic |
| GTGGACCAGTGCGCCAATGG+TGG | 0.584793 | 1.3:-57153740 | MS.gene43995:CDS |
| CAAATTGATACCAATGGACA+GGG | 0.591064 | 1.3:-57153689 | MS.gene43995:CDS |
| TCAAGTAACAGTGAGAATAG+TGG | 0.598835 | 1.3:-57153759 | MS.gene43995:CDS |
| CAGTGTATACTGTGCTACCT+GGG | 0.602353 | 1.3:-57154385 | MS.gene43995:CDS |
| CAATGGACAGGGTTACCAGA+AGG | 0.617429 | 1.3:-57153678 | MS.gene43995:CDS |
| TGTGGACCCCAGGGCCCTCG+TGG | 0.633299 | 1.3:-57154312 | MS.gene43995:CDS |
| GTGGGAACGATGTTAGTGAA+TGG | 0.656794 | 1.3:-57154483 | None:intergenic |
| TCTCTACCACGAGGGCCCTG+GGG | 0.658114 | 1.3:+57154306 | None:intergenic |
| CCAGGTAGCACAGTATACAC+TGG | 0.672657 | 1.3:+57154386 | None:intergenic |
| CCTCGTGGTAGAGAGTCTTG+TGG | 0.684944 | 1.3:-57154297 | MS.gene43995:CDS |
| CACAAGACTCTCTACCACGA+GGG | 0.694332 | 1.3:+57154298 | None:intergenic |
| CCACAAGACTCTCTACCACG+AGG | 0.710327 | 1.3:+57154297 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTTATAACACATTTATAATT+AGG | - | chr1.3:57154102-57154121 | MS.gene43995:intron | 10.0% |
| !!! | TTTTATAAAATGAGTAAGTA+CGG | + | chr1.3:57153862-57153881 | None:intergenic | 15.0% |
| !! | ACCTAAACACATTTATAATT+AGG | - | chr1.3:57154025-57154044 | MS.gene43995:intron | 20.0% |
| !! | ACCTAATTATAAATGTGTTT+AGG | + | chr1.3:57154029-57154048 | None:intergenic | 20.0% |
| !! | GATAGTTGAATTAATATTAG+TGG | - | chr1.3:57154298-57154317 | MS.gene43995:CDS | 20.0% |
| ! | AATCCCAATTAATATTCTGA+GGG | + | chr1.3:57153699-57153718 | None:intergenic | 25.0% |
| ! | CAAAGACATAGTTAACTATA+AGG | + | chr1.3:57154466-57154485 | None:intergenic | 25.0% |
| ! | TAATCCCAATTAATATTCTG+AGG | + | chr1.3:57153700-57153719 | None:intergenic | 25.0% |
| ! | TTCAATCAAATTGATACCAA+TGG | - | chr1.3:57154419-57154438 | MS.gene43995:CDS | 25.0% |
| !! | ATTAGGTCATAAATCGTTTT+CGG | - | chr1.3:57154042-57154061 | MS.gene43995:intron | 25.0% |
| !!! | ATCTCTATGAGATTTTTTGT+CGG | - | chr1.3:57154214-57154233 | MS.gene43995:intron | 25.0% |
| ACAACCCTCAGAATATTAAT+TGG | - | chr1.3:57153692-57153711 | MS.gene43995:CDS | 30.0% | |
| CAACCCTCAGAATATTAATT+GGG | - | chr1.3:57153693-57153712 | MS.gene43995:CDS | 30.0% | |
| ! | AATATTAGTGGTGATGATAC+AGG | - | chr1.3:57154310-57154329 | MS.gene43995:CDS | 30.0% |
| ! | ATTAGGTCATAAACCGTTTT+CGG | - | chr1.3:57154119-57154138 | MS.gene43995:intron | 30.0% |
| ! | TAATATTCTGAGGGTTGTAA+AGG | + | chr1.3:57153690-57153709 | None:intergenic | 30.0% |
| !! | ATTCAACTCAGTTTTAGTCA+AGG | - | chr1.3:57154253-57154272 | MS.gene43995:intron | 30.0% |
| !!! | TCTCTATGAGATTTTTTGTC+GGG | - | chr1.3:57154215-57154234 | MS.gene43995:intron | 30.0% |
| CAAATTGATACCAATGGACA+GGG | - | chr1.3:57154425-57154444 | MS.gene43995:CDS | 35.0% | |
| TCAAATTGATACCAATGGAC+AGG | - | chr1.3:57154424-57154443 | MS.gene43995:CDS | 35.0% | |
| TCAAGTAACAGTGAGAATAG+TGG | - | chr1.3:57154355-57154374 | MS.gene43995:CDS | 35.0% | |
| TCATAGAAACAAGCCGAAAA+CGG | + | chr1.3:57154058-57154077 | None:intergenic | 35.0% | |
| TTGGAATGGCGTAAAAAGTA+TGG | - | chr1.3:57153766-57153785 | MS.gene43995:CDS | 35.0% | |
| ! | AATGGCGTAAAAAGTATGGT+TGG | - | chr1.3:57153770-57153789 | MS.gene43995:CDS | 35.0% |
| !! | ATACTTTTTACGCCATTCCA+AGG | + | chr1.3:57153767-57153786 | None:intergenic | 35.0% |
| !! | TACTTTTTACGCCATTCCAA+GGG | + | chr1.3:57153766-57153785 | None:intergenic | 35.0% |
| !!! | CTCTATGAGATTTTTTGTCG+GGG | - | chr1.3:57154216-57154235 | MS.gene43995:intron | 35.0% |
| !!! | TCTATGAGATTTTTTGTCGG+GGG | - | chr1.3:57154217-57154236 | MS.gene43995:intron | 35.0% |
| ATCCAAGTCTAATCCACCAT+TGG | + | chr1.3:57154390-57154409 | None:intergenic | 40.0% | |
| TAACTATAAGGTGACCCTTC+TGG | + | chr1.3:57154454-57154473 | None:intergenic | 40.0% | |
| ! | TATTCTGAGGGTTGTAAAGG+TGG | + | chr1.3:57153687-57153706 | None:intergenic | 40.0% |
| AATGGACAGGGTTACCAGAA+GGG | - | chr1.3:57154437-57154456 | MS.gene43995:CDS | 45.0% | |
| CAGGTGACAAATACTGCAAC+TGG | - | chr1.3:57154329-57154348 | MS.gene43995:CDS | 45.0% | |
| CAGTGTATACTGTGCTACCT+GGG | - | chr1.3:57153729-57153748 | MS.gene43995:CDS | 45.0% | |
| GTGAATGGTCAGAGTGCAAA+CGG | - | chr1.3:57153646-57153665 | MS.gene43995:CDS | 45.0% | |
| ! | TATGGTTGGACTGCCTTTTG+TGG | - | chr1.3:57153784-57153803 | MS.gene43995:CDS | 45.0% |
| ATGCAAACCAGCCCTTGGAA+TGG | - | chr1.3:57153752-57153771 | MS.gene43995:CDS | 50.0% | |
| CAATGGACAGGGTTACCAGA+AGG | - | chr1.3:57154436-57154455 | MS.gene43995:CDS | 50.0% | |
| CACAAGACTCTCTACCACGA+GGG | + | chr1.3:57153819-57153838 | None:intergenic | 50.0% | |
| CCAGGTAGCACAGTATACAC+TGG | + | chr1.3:57153731-57153750 | None:intergenic | 50.0% | |
| CCAGTGTATACTGTGCTACC+TGG | - | chr1.3:57153728-57153747 | MS.gene43995:CDS | 50.0% | |
| CTTCTGGTAACCCTGTCCAT+TGG | + | chr1.3:57154438-57154457 | None:intergenic | 50.0% | |
| GTCAGAGTGCAAACGGTGTA+AGG | - | chr1.3:57153653-57153672 | MS.gene43995:CDS | 50.0% | |
| GTCTTGTGGCAAGTGCTTGA+GGG | - | chr1.3:57153831-57153850 | MS.gene43995:intron | 50.0% | |
| ! | AGTCTTGTGGCAAGTGCTTG+AGG | - | chr1.3:57153830-57153849 | MS.gene43995:intron | 50.0% |
| ! | TTTTACGCCATTCCAAGGGC+TGG | + | chr1.3:57153762-57153781 | None:intergenic | 50.0% |
| !! | CGCCAATGGTGGATTAGACT+TGG | - | chr1.3:57154385-57154404 | MS.gene43995:CDS | 50.0% |
| CCACAAGACTCTCTACCACG+AGG | + | chr1.3:57153820-57153839 | None:intergenic | 55.0% | |
| CCTCGTGGTAGAGAGTCTTG+TGG | - | chr1.3:57153817-57153836 | MS.gene43995:intron | 55.0% | |
| CTAATCCACCATTGGCGCAC+TGG | + | chr1.3:57154382-57154401 | None:intergenic | 55.0% | |
| ! | ACTGCCTTTTGTGGACCCCA+GGG | - | chr1.3:57153793-57153812 | MS.gene43995:intron | 55.0% |
| !! | ATAGTGGACCAGTGCGCCAA+TGG | - | chr1.3:57154371-57154390 | MS.gene43995:CDS | 55.0% |
| CTGGGATGCAAACCAGCCCT+TGG | - | chr1.3:57153747-57153766 | MS.gene43995:CDS | 60.0% | |
| ! | GACTGCCTTTTGTGGACCCC+AGG | - | chr1.3:57153792-57153811 | MS.gene43995:intron | 60.0% |
| !! | CAAGGGCTGGTTTGCATCCC+AGG | + | chr1.3:57153749-57153768 | None:intergenic | 60.0% |
| ACTCTCTACCACGAGGGCCC+TGG | + | chr1.3:57153813-57153832 | None:intergenic | 65.0% | |
| AGGGCCCTGGGGTCCACAAA+AGG | + | chr1.3:57153800-57153819 | None:intergenic | 65.0% | |
| CTCTCTACCACGAGGGCCCT+GGG | + | chr1.3:57153812-57153831 | None:intergenic | 65.0% | |
| GTGGACCAGTGCGCCAATGG+TGG | - | chr1.3:57154374-57154393 | MS.gene43995:CDS | 65.0% | |
| TCTCTACCACGAGGGCCCTG+GGG | + | chr1.3:57153811-57153830 | None:intergenic | 65.0% | |
| TGTGGACCCCAGGGCCCTCG+TGG | - | chr1.3:57153802-57153821 | MS.gene43995:intron | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 57153640 | 57154496 | 57153640 | ID=MS.gene43995 |
| chr1.3 | mRNA | 57153640 | 57154496 | 57153640 | ID=MS.gene43995.t1;Parent=MS.gene43995 |
| chr1.3 | exon | 57154284 | 57154496 | 57154284 | ID=MS.gene43995.t1.exon1;Parent=MS.gene43995.t1 |
| chr1.3 | CDS | 57154284 | 57154496 | 57154284 | ID=cds.MS.gene43995.t1;Parent=MS.gene43995.t1 |
| chr1.3 | exon | 57153640 | 57153804 | 57153640 | ID=MS.gene43995.t1.exon2;Parent=MS.gene43995.t1 |
| chr1.3 | CDS | 57153640 | 57153804 | 57153640 | ID=cds.MS.gene43995.t1;Parent=MS.gene43995.t1 |
| Gene Sequence |
| Protein sequence |
