Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049701 | MS.gene44743 | 0.808756 | 2.65E-50 | -1.69E-46 |
| MS.gene049853 | MS.gene44743 | 0.803091 | 4.11E-49 | -1.69E-46 |
| MS.gene051710 | MS.gene44743 | 0.816878 | 4.41E-52 | -1.69E-46 |
| MS.gene052456 | MS.gene44743 | 0.803526 | 3.34E-49 | -1.69E-46 |
| MS.gene054801 | MS.gene44743 | 0.823149 | 1.62E-53 | -1.69E-46 |
| MS.gene055056 | MS.gene44743 | 0.817912 | 2.58E-52 | -1.69E-46 |
| MS.gene055058 | MS.gene44743 | 0.811385 | 7.19E-51 | -1.69E-46 |
| MS.gene056252 | MS.gene44743 | 0.80103 | 1.09E-48 | -1.69E-46 |
| MS.gene058515 | MS.gene44743 | 0.854682 | 1.05E-61 | -1.69E-46 |
| MS.gene059276 | MS.gene44743 | 0.823324 | 1.47E-53 | -1.69E-46 |
| MS.gene059584 | MS.gene44743 | 0.810696 | 1.01E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 25 sgRNAs with CRISPR-Local
Find 89 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATTCTCTGGCGGCCGAAAA+TGG | 0.315874 | 7.4:+82910994 | MS.gene44743:CDS |
| GGTTGCATCGACGATGAATA+TGG | 0.356657 | 7.4:-82910208 | None:intergenic |
| CTTCAAACTTGACTTCCATA+TGG | 0.406172 | 7.4:-82910602 | None:intergenic |
| CCATTGATTGTCGGTGGCAG+TGG | 0.410840 | 7.4:-82910258 | None:intergenic |
| AAGCTTAAAAGTTGTTGAAA+AGG | 0.425063 | 7.4:+82909842 | MS.gene44743:CDS |
| GAGAGGAACAAACTTTGAGT+TGG | 0.491050 | 7.4:-82910289 | None:intergenic |
| GAAATGTGAAATTATCCATA+TGG | 0.491863 | 7.4:+82910587 | MS.gene44743:CDS |
| CCACTGCCACCGACAATCAA+TGG | 0.492411 | 7.4:+82910258 | MS.gene44743:CDS |
| CTTAGTTCATCGGGCTCCAT+CGG | 0.496160 | 7.4:-82909647 | None:intergenic |
| CTTAGTTCATCGGGCTCCAT+CGG | 0.496160 | 7.4:-82911022 | None:intergenic |
| ACAACTGCTCTTAGTTCATC+GGG | 0.500188 | 7.4:-82909656 | None:intergenic |
| ACAACTGCTCTTAGTTCATC+GGG | 0.500188 | 7.4:-82911031 | None:intergenic |
| GAATATAAACCATTGATTGT+CGG | 0.510162 | 7.4:-82910267 | None:intergenic |
| GACAACTGCTCTTAGTTCAT+CGG | 0.519655 | 7.4:-82909657 | None:intergenic |
| AGTGGATTCATCGGTAGTGA+AGG | 0.551513 | 7.4:-82910240 | None:intergenic |
| CATGAATGTCAAAATTCTGA+AGG | 0.559473 | 7.4:-82909679 | None:intergenic |
| GTCGGTGGCAGTGGATTCAT+CGG | 0.560428 | 7.4:-82910249 | None:intergenic |
| GCCGAAAATGGTATCACCGA+TGG | 0.562882 | 7.4:+82909631 | None:intergenic |
| GCCGAAAATGGTATCACCGA+TGG | 0.562882 | 7.4:+82911006 | MS.gene44743:CDS |
| CGGTAGTGAAGGGAGAGGAT+CGG | 0.619372 | 7.4:-82910229 | None:intergenic |
| GTGGATTCATCGGTAGTGAA+GGG | 0.624447 | 7.4:-82910239 | None:intergenic |
| TATAAACCATTGATTGTCGG+TGG | 0.633321 | 7.4:-82910264 | None:intergenic |
| ATCGACGATGAATATGGTGT+CGG | 0.635273 | 7.4:-82910202 | None:intergenic |
| TTCATCGGTAGTGAAGGGAG+AGG | 0.660200 | 7.4:-82910234 | None:intergenic |
| TAAACTTCAGATATGACACA+TGG | 0.660343 | 7.4:+82909807 | MS.gene44743:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTTAATTGAAAAAACATT+AGG | - | chr7.4:82910378-82910397 | None:intergenic | 10.0% |
| !!! | TGCAATAATTATTTTCAAAT+TGG | - | chr7.4:82910896-82910915 | None:intergenic | 15.0% |
| !! | AAAAATAAAATAAGGTGAGA+TGG | - | chr7.4:82910930-82910949 | None:intergenic | 20.0% |
| !! | AAATACGATAAAAATAGATG+AGG | - | chr7.4:82909747-82909766 | None:intergenic | 20.0% |
| !! | GTGCTGAAAAAAATAAAATA+AGG | - | chr7.4:82910938-82910957 | None:intergenic | 20.0% |
| !! | TAGTTGTAAAATGAAAAACA+CGG | - | chr7.4:82910727-82910746 | None:intergenic | 20.0% |
| !! | TTTCAATCAAATTATTGCTT+AGG | + | chr7.4:82910346-82910365 | MS.gene44743:intron | 20.0% |
| !!! | AAAGTAAGTACAATTTTCAT+TGG | - | chr7.4:82909712-82909731 | None:intergenic | 20.0% |
| !!! | AAGTAAGTACAATTTTCATT+GGG | - | chr7.4:82909711-82909730 | None:intergenic | 20.0% |
| !!! | TATCGTATTTCTTATTTTAC+AGG | + | chr7.4:82909757-82909776 | MS.gene44743:intron | 20.0% |
| !!! | TTAAAATTCTTCATCTTTTC+TGG | + | chr7.4:82910391-82910410 | MS.gene44743:intron | 20.0% |
| !!! | TTTTTAGATGATCATATCAT+AGG | + | chr7.4:82910682-82910701 | MS.gene44743:intron | 20.0% |
| ! | AAGCTTAAAAGTTGTTGAAA+AGG | + | chr7.4:82909842-82909861 | MS.gene44743:CDS | 25.0% |
| ! | AGGTTTAGTAATTAAGCTTT+AGG | - | chr7.4:82909987-82910006 | None:intergenic | 25.0% |
| ! | ATATATAGAATAGAACACAG+TGG | - | chr7.4:82909887-82909906 | None:intergenic | 25.0% |
| ! | CAATAGTAAAAAGAATTCTC+TGG | + | chr7.4:82910981-82911000 | MS.gene44743:CDS | 25.0% |
| ! | GAAATGTGAAATTATCCATA+TGG | + | chr7.4:82910587-82910606 | MS.gene44743:CDS | 25.0% |
| ! | TAAAATTAGGGCTTCAATAT+TGG | - | chr7.4:82909925-82909944 | None:intergenic | 25.0% |
| !! | ACAAGTATTTAGTTTACACT+TGG | + | chr7.4:82910838-82910857 | MS.gene44743:intron | 25.0% |
| !! | CTAGGTTTAGGTTAAAATTA+GGG | - | chr7.4:82909937-82909956 | None:intergenic | 25.0% |
| !! | GAATATAAACCATTGATTGT+CGG | - | chr7.4:82910270-82910289 | None:intergenic | 25.0% |
| !! | GTTAGGTTTAGGTTTAAATT+AGG | - | chr7.4:82910007-82910026 | None:intergenic | 25.0% |
| !! | TCAACAACTTTTAAGCTTTT+GGG | - | chr7.4:82909840-82909859 | None:intergenic | 25.0% |
| !! | TTCAACAACTTTTAAGCTTT+TGG | - | chr7.4:82909841-82909860 | None:intergenic | 25.0% |
| !!! | ACAACTAATTGTTTTATGCT+AGG | + | chr7.4:82910740-82910759 | MS.gene44743:intron | 25.0% |
| !!! | CTAATTTTAACCTAAACCTA+GGG | + | chr7.4:82909936-82909955 | MS.gene44743:intron | 25.0% |
| !!! | GTATTTCTTATTTTACAGGT+AGG | + | chr7.4:82909761-82909780 | MS.gene44743:intron | 25.0% |
| !!! | TTATTTTTTTCAGCACATCA+TGG | + | chr7.4:82910942-82910961 | MS.gene44743:intron | 25.0% |
| AAACAGGAAATTTAATCCCT+AGG | - | chr7.4:82909955-82909974 | None:intergenic | 30.0% | |
| AATAAAATAAGGTGAGATGG+TGG | - | chr7.4:82910927-82910946 | None:intergenic | 30.0% | |
| CATGAATGTCAAAATTCTGA+AGG | - | chr7.4:82909682-82909701 | None:intergenic | 30.0% | |
| GAAATTTAATCCCTAGGTTT+AGG | - | chr7.4:82909949-82909968 | None:intergenic | 30.0% | |
| GATATTACTCTACACTGATA+TGG | + | chr7.4:82910765-82910784 | MS.gene44743:intron | 30.0% | |
| TAAACTTCAGATATGACACA+TGG | + | chr7.4:82909807-82909826 | MS.gene44743:intron | 30.0% | |
| TAGTAAAAAGAATTCTCTGG+CGG | + | chr7.4:82910984-82911003 | MS.gene44743:CDS | 30.0% | |
| TCTAAACTGGATCAAAAAAG+TGG | - | chr7.4:82910150-82910169 | None:intergenic | 30.0% | |
| TTACAGGTAGGTGTTTATTA+AGG | + | chr7.4:82909773-82909792 | MS.gene44743:intron | 30.0% | |
| TTTCACAACCTGTAAATAAG+AGG | - | chr7.4:82910571-82910590 | None:intergenic | 30.0% | |
| ! | CCTAGGTTTAGGTTAAAATT+AGG | - | chr7.4:82909938-82909957 | None:intergenic | 30.0% |
| ! | TATTTTCAAATTGGCATCTG+CGG | - | chr7.4:82910887-82910906 | None:intergenic | 30.0% |
| !! | AGTAATTAAGCTTTAGGTCA+GGG | - | chr7.4:82909981-82910000 | None:intergenic | 30.0% |
| !! | CCTAATTTTAACCTAAACCT+AGG | + | chr7.4:82909935-82909954 | MS.gene44743:intron | 30.0% |
| !! | CTTCAGAATTTTGACATTCA+TGG | + | chr7.4:82909680-82909699 | MS.gene44743:CDS | 30.0% |
| !! | GTCTTTTTCCTCTTATTTAC+AGG | + | chr7.4:82910560-82910579 | MS.gene44743:intron | 30.0% |
| !! | TAGTAATTAAGCTTTAGGTC+AGG | - | chr7.4:82909982-82910001 | None:intergenic | 30.0% |
| !!! | CTTTTTTGATCCAGTTTAGA+AGG | + | chr7.4:82910150-82910169 | MS.gene44743:intron | 30.0% |
| !!! | TGAAGCTTCTATACTTTTTC+AGG | + | chr7.4:82910620-82910639 | MS.gene44743:CDS | 30.0% |
| ACGAAAATGAAGATGAGAGA+TGG | - | chr7.4:82910320-82910339 | None:intergenic | 35.0% | |
| ATCAAAAAAGTGGAGTGAGA+GGG | - | chr7.4:82910140-82910159 | None:intergenic | 35.0% | |
| CACCATTAAAGTCGATATAG+AGG | - | chr7.4:82910806-82910825 | None:intergenic | 35.0% | |
| CTTCAAACTTGACTTCCATA+TGG | - | chr7.4:82910605-82910624 | None:intergenic | 35.0% | |
| GAAAGAGGGGAAAAATTGTT+AGG | - | chr7.4:82910024-82910043 | None:intergenic | 35.0% | |
| GGGGAAAAATTGTTAGGTTT+AGG | - | chr7.4:82910018-82910037 | None:intergenic | 35.0% | |
| ! | ACACTTTTACATACACCTTG+TGG | - | chr7.4:82910507-82910526 | None:intergenic | 35.0% |
| ! | CACTTTTACATACACCTTGT+GGG | - | chr7.4:82910506-82910525 | None:intergenic | 35.0% |
| ! | TATAAACCATTGATTGTCGG+TGG | - | chr7.4:82910267-82910286 | None:intergenic | 35.0% |
| !! | GAATTTTGACATTCATGGTG+AGG | + | chr7.4:82909685-82909704 | MS.gene44743:intron | 35.0% |
| !! | TGCCTCTATATCGACTTTAA+TGG | + | chr7.4:82910801-82910820 | MS.gene44743:intron | 35.0% |
| !!! | CTTCTATACTTTTTCAGGTG+AGG | + | chr7.4:82910625-82910644 | MS.gene44743:intron | 35.0% |
| ACAACTGCTCTTAGTTCATC+GGG | - | chr7.4:82909659-82909678 | None:intergenic | 40.0% | |
| ATCGACGATGAATATGGTGT+CGG | - | chr7.4:82910205-82910224 | None:intergenic | 40.0% | |
| CTTTAGGTCAGGGTTAAAAC+AGG | - | chr7.4:82909971-82909990 | None:intergenic | 40.0% | |
| GACAACTGCTCTTAGTTCAT+CGG | - | chr7.4:82909660-82909679 | None:intergenic | 40.0% | |
| GAGAGGAACAAACTTTGAGT+TGG | - | chr7.4:82910292-82910311 | None:intergenic | 40.0% | |
| GATCAAAAAAGTGGAGTGAG+AGG | - | chr7.4:82910141-82910160 | None:intergenic | 40.0% | |
| ! | TCCATCGGTGATACCATTTT+CGG | - | chr7.4:82911010-82911029 | None:intergenic | 40.0% |
| ! | TTAGGGCTTCAATATTGGCT+TGG | - | chr7.4:82909920-82909939 | None:intergenic | 40.0% |
| !! | TAGGGCTTCAATATTGGCTT+GGG | - | chr7.4:82909919-82909938 | None:intergenic | 40.0% |
| AAGAAACGCGTGAGAGAAAG+AGG | - | chr7.4:82910039-82910058 | None:intergenic | 45.0% | |
| AGAAACGCGTGAGAGAAAGA+GGG | - | chr7.4:82910038-82910057 | None:intergenic | 45.0% | |
| GAGAGAGATGCCTTCTAAAC+TGG | - | chr7.4:82910163-82910182 | None:intergenic | 45.0% | |
| GTGAAACTCAGATGAGAGTG+CGG | - | chr7.4:82910115-82910134 | None:intergenic | 45.0% | |
| GTGGATTCATCGGTAGTGAA+GGG | - | chr7.4:82910242-82910261 | None:intergenic | 45.0% | |
| ! | AGTGGATTCATCGGTAGTGA+AGG | - | chr7.4:82910243-82910262 | None:intergenic | 45.0% |
| ! | GCTGATTTCTTCAACCCACA+AGG | + | chr7.4:82910489-82910508 | MS.gene44743:intron | 45.0% |
| ! | GGTTGCATCGACGATGAATA+TGG | - | chr7.4:82910211-82910230 | None:intergenic | 45.0% |
| AATTCTCTGGCGGCCGAAAA+TGG | + | chr7.4:82910994-82911013 | MS.gene44743:CDS | 50.0% | |
| CTTAGTTCATCGGGCTCCAT+CGG | - | chr7.4:82909650-82909669 | None:intergenic | 50.0% | |
| GAAACGCGTGAGAGAAAGAG+GGG | - | chr7.4:82910037-82910056 | None:intergenic | 50.0% | |
| GATGAGAGATGGAGAGAGAG+AGG | - | chr7.4:82910309-82910328 | None:intergenic | 50.0% | |
| GCCGAAAATGGTATCACCGA+TGG | + | chr7.4:82911006-82911025 | MS.gene44743:CDS | 50.0% | |
| TTCATCGGTAGTGAAGGGAG+AGG | - | chr7.4:82910237-82910256 | None:intergenic | 50.0% | |
| AGCGTCCTCAGATGAGAGTG+TGG | - | chr7.4:82910079-82910098 | None:intergenic | 55.0% | |
| CCACTGCCACCGACAATCAA+TGG | + | chr7.4:82910258-82910277 | MS.gene44743:CDS | 55.0% | |
| CGGTAGTGAAGGGAGAGGAT+CGG | - | chr7.4:82910232-82910251 | None:intergenic | 55.0% | |
| GCGATCCACACTCTCATCTG+AGG | + | chr7.4:82910071-82910090 | MS.gene44743:intron | 55.0% | |
| ! | CCATTGATTGTCGGTGGCAG+TGG | - | chr7.4:82910261-82910280 | None:intergenic | 55.0% |
| ! | GTCGGTGGCAGTGGATTCAT+CGG | - | chr7.4:82910252-82910271 | None:intergenic | 55.0% |
| CACCGATGGAGCCCGATGAA+TGG | + | chr7.4:82909645-82909664 | MS.gene44743:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 82909638 | 82911038 | 82909638 | ID=MS.gene44743 |
| chr7.4 | mRNA | 82909638 | 82911038 | 82909638 | ID=MS.gene44743.t1;Parent=MS.gene44743 |
| chr7.4 | exon | 82909638 | 82909701 | 82909638 | ID=MS.gene44743.t1.exon1;Parent=MS.gene44743.t1 |
| chr7.4 | CDS | 82909638 | 82909701 | 82909638 | ID=cds.MS.gene44743.t1;Parent=MS.gene44743.t1 |
| chr7.4 | exon | 82909817 | 82909863 | 82909817 | ID=MS.gene44743.t1.exon2;Parent=MS.gene44743.t1 |
| chr7.4 | CDS | 82909817 | 82909863 | 82909817 | ID=cds.MS.gene44743.t1;Parent=MS.gene44743.t1 |
| chr7.4 | exon | 82910164 | 82910298 | 82910164 | ID=MS.gene44743.t1.exon3;Parent=MS.gene44743.t1 |
| chr7.4 | CDS | 82910164 | 82910298 | 82910164 | ID=cds.MS.gene44743.t1;Parent=MS.gene44743.t1 |
| chr7.4 | exon | 82910582 | 82910641 | 82910582 | ID=MS.gene44743.t1.exon4;Parent=MS.gene44743.t1 |
| chr7.4 | CDS | 82910582 | 82910641 | 82910582 | ID=cds.MS.gene44743.t1;Parent=MS.gene44743.t1 |
| chr7.4 | exon | 82910955 | 82911038 | 82910955 | ID=MS.gene44743.t1.exon5;Parent=MS.gene44743.t1 |
| chr7.4 | CDS | 82910955 | 82911038 | 82910955 | ID=cds.MS.gene44743.t1;Parent=MS.gene44743.t1 |
| Gene Sequence |
| Protein sequence |
