Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45439.t1 | XP_013443957.1 | 96.6 | 412 | 14 | 0 | 1 | 412 | 242 | 653 | 2.60E-240 | 840.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45439.t1 | F4HVY0 | 59.7 | 414 | 161 | 3 | 3 | 411 | 208 | 620 | 9.5e-151 | 534.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45439.t1 | A0A072TKE8 | 96.6 | 412 | 14 | 0 | 1 | 412 | 242 | 653 | 1.8e-240 | 840.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049260 | MS.gene45439 | 0.801419 | 9.09E-49 | -1.69E-46 |
| MS.gene051626 | MS.gene45439 | 0.850498 | 1.63E-60 | -1.69E-46 |
| MS.gene051789 | MS.gene45439 | 0.868135 | 8.18E-66 | -1.69E-46 |
| MS.gene052178 | MS.gene45439 | 0.804178 | 2.45E-49 | -1.69E-46 |
| MS.gene052967 | MS.gene45439 | 0.81617 | 6.35E-52 | -1.69E-46 |
| MS.gene053074 | MS.gene45439 | 0.86007 | 2.67E-63 | -1.69E-46 |
| MS.gene053630 | MS.gene45439 | 0.821408 | 4.10E-53 | -1.69E-46 |
| MS.gene054184 | MS.gene45439 | 0.829463 | 5.08E-55 | -1.69E-46 |
| MS.gene054185 | MS.gene45439 | 0.837635 | 4.63E-57 | -1.69E-46 |
| MS.gene054596 | MS.gene45439 | 0.822667 | 2.10E-53 | -1.69E-46 |
| MS.gene055207 | MS.gene45439 | 0.818838 | 1.59E-52 | -1.69E-46 |
| MS.gene057754 | MS.gene45439 | 0.817427 | 3.32E-52 | -1.69E-46 |
| MS.gene058168 | MS.gene45439 | 0.83892 | 2.16E-57 | -1.69E-46 |
| MS.gene059006 | MS.gene45439 | 0.860589 | 1.86E-63 | -1.69E-46 |
| MS.gene059228 | MS.gene45439 | 0.817921 | 2.57E-52 | -1.69E-46 |
| MS.gene060476 | MS.gene45439 | 0.806903 | 6.56E-50 | -1.69E-46 |
| MS.gene061113 | MS.gene45439 | 0.81496 | 1.18E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45439.t1 | MTR_8g009590 | 96.602 | 412 | 14 | 0 | 1 | 412 | 242 | 653 | 0.0 | 842 |
| MS.gene45439.t1 | MTR_8g009560 | 63.747 | 411 | 148 | 1 | 1 | 411 | 206 | 615 | 0.0 | 556 |
| MS.gene45439.t1 | MTR_7g090100 | 59.854 | 411 | 161 | 2 | 1 | 411 | 203 | 609 | 0.0 | 536 |
| MS.gene45439.t1 | MTR_7g090120 | 60.097 | 411 | 162 | 2 | 1 | 411 | 203 | 611 | 0.0 | 530 |
| MS.gene45439.t1 | MTR_7g090140 | 58.637 | 411 | 167 | 2 | 1 | 411 | 108 | 515 | 0.0 | 526 |
| MS.gene45439.t1 | MTR_7g090140 | 58.637 | 411 | 167 | 2 | 1 | 411 | 203 | 610 | 0.0 | 524 |
| MS.gene45439.t1 | MTR_4g054290 | 59.124 | 411 | 166 | 2 | 1 | 411 | 203 | 611 | 0.0 | 524 |
| MS.gene45439.t1 | MTR_4g054150 | 59.413 | 409 | 164 | 2 | 3 | 411 | 184 | 590 | 0.0 | 521 |
| MS.gene45439.t1 | MTR_4g129630 | 39.048 | 420 | 245 | 5 | 3 | 412 | 207 | 625 | 8.66e-94 | 295 |
| MS.gene45439.t1 | MTR_4g054320 | 64.706 | 51 | 18 | 0 | 361 | 411 | 43 | 93 | 1.93e-20 | 85.9 |
| MS.gene45439.t1 | MTR_8g009550 | 74.359 | 39 | 10 | 0 | 3 | 41 | 49 | 87 | 5.14e-14 | 67.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45439.t1 | AT1G02205 | 59.518 | 415 | 162 | 3 | 2 | 411 | 43 | 456 | 0.0 | 543 |
| MS.gene45439.t1 | AT1G02205 | 59.375 | 416 | 163 | 3 | 1 | 411 | 207 | 621 | 0.0 | 540 |
| MS.gene45439.t1 | AT1G02205 | 59.375 | 416 | 163 | 3 | 1 | 411 | 206 | 620 | 0.0 | 538 |
| MS.gene45439.t1 | AT1G02205 | 58.907 | 421 | 162 | 3 | 1 | 411 | 206 | 625 | 0.0 | 538 |
| MS.gene45439.t1 | AT2G37700 | 58.738 | 412 | 165 | 3 | 4 | 411 | 205 | 615 | 0.0 | 520 |
| MS.gene45439.t1 | AT2G37700 | 58.252 | 412 | 158 | 4 | 4 | 411 | 205 | 606 | 3.36e-178 | 510 |
| MS.gene45439.t1 | AT1G02205 | 58.133 | 375 | 151 | 3 | 42 | 411 | 8 | 381 | 1.03e-166 | 473 |
| MS.gene45439.t1 | AT1G02190 | 53.828 | 418 | 185 | 5 | 1 | 411 | 206 | 622 | 4.98e-166 | 480 |
| MS.gene45439.t1 | AT1G02190 | 53.349 | 418 | 183 | 6 | 1 | 411 | 206 | 618 | 6.25e-163 | 472 |
| MS.gene45439.t1 | AT2G37700 | 57.180 | 383 | 159 | 3 | 33 | 411 | 197 | 578 | 6.02e-160 | 463 |
| MS.gene45439.t1 | AT2G37700 | 60.364 | 275 | 107 | 2 | 4 | 277 | 205 | 478 | 5.27e-120 | 358 |
| MS.gene45439.t1 | AT5G57800 | 38.571 | 420 | 247 | 5 | 3 | 412 | 210 | 628 | 1.17e-92 | 292 |
Find 92 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCACTTTATGACTACATTTA+TGG | 0.018854 | 8.4:+78419188 | MS.gene45439:CDS |
| CTTAGATGATAAATGGATTC+TGG | 0.234003 | 8.4:-78419305 | None:intergenic |
| TCCAAATCTTAGATGATAAA+TGG | 0.234271 | 8.4:-78419312 | None:intergenic |
| TCCATTTATCATCTAAGATT+TGG | 0.244366 | 8.4:+78419311 | MS.gene45439:CDS |
| AATACTTGAGTTGTTCCTTT+TGG | 0.251616 | 8.4:-78422390 | None:intergenic |
| GGAATAAATAATGTTCCTTT+TGG | 0.298446 | 8.4:-78423347 | None:intergenic |
| CAGTCTTTGCGGTGTTTCTT+TGG | 0.302513 | 8.4:-78423386 | None:intergenic |
| TTTGGTGGGAATTGTGAGTA+TGG | 0.332345 | 8.4:-78423368 | None:intergenic |
| TGATTAACATGGACAAAGTT+TGG | 0.333533 | 8.4:+78424078 | MS.gene45439:CDS |
| GAGGAAGAGCTTAACATCTA+TGG | 0.339405 | 8.4:+78422279 | MS.gene45439:CDS |
| GAAGTGGGAGTGAGCATTGC+AGG | 0.340128 | 8.4:-78423425 | None:intergenic |
| ACAACCTTAACATTAAGTTT+TGG | 0.350187 | 8.4:-78422324 | None:intergenic |
| AATGATTGAGGAAGCAATAC+TGG | 0.354584 | 8.4:+78422206 | MS.gene45439:CDS |
| CTTAATGTTAAGGTTGTAGA+TGG | 0.357126 | 8.4:+78422330 | MS.gene45439:CDS |
| AGTTTGTTCTAAACTGAGTA+TGG | 0.358466 | 8.4:-78419151 | None:intergenic |
| TTAGATGATAAATGGATTCT+GGG | 0.378665 | 8.4:-78419304 | None:intergenic |
| ATGAGTGCATGGCGCATAGC+TGG | 0.389798 | 8.4:+78424005 | MS.gene45439:CDS |
| AATTGACCTGAACTCCTTGT+TGG | 0.393585 | 8.4:-78422464 | None:intergenic |
| ACAAACTTAACCTTCAAACT+TGG | 0.410759 | 8.4:+78419468 | MS.gene45439:CDS |
| TGATTATGTGAAGCTCAAAA+AGG | 0.413366 | 8.4:+78422642 | MS.gene45439:CDS |
| CTTTGCGGTGTTTCTTTGGT+GGG | 0.417923 | 8.4:-78423382 | None:intergenic |
| AACATGGACCAAGCTGTAAC+AGG | 0.421721 | 8.4:-78419392 | None:intergenic |
| AAGATTTGGATTTGCTGCCT+TGG | 0.427781 | 8.4:+78419325 | MS.gene45439:CDS |
| GCATCCAAAACTTAATGTTA+AGG | 0.437243 | 8.4:+78422320 | MS.gene45439:CDS |
| ACACATCTAAGTGGTACCTT+TGG | 0.442501 | 8.4:+78419360 | MS.gene45439:CDS |
| ATATACCTGAACACTGTACT+TGG | 0.444663 | 8.4:-78419498 | None:intergenic |
| TGTGTTAAGTGGACAACATT+TGG | 0.453059 | 8.4:-78419272 | None:intergenic |
| TAGATTTGGTTAGTGGGAGA+TGG | 0.463206 | 8.4:+78423296 | MS.gene45439:intron |
| GGCAATAGCATAAGCAACTT+TGG | 0.472073 | 8.4:-78422430 | None:intergenic |
| TGGGTATTGCCCAAGTTTGA+AGG | 0.477456 | 8.4:-78419478 | None:intergenic |
| TTCTGCATAGATTTGGTTAG+TGG | 0.479155 | 8.4:+78423289 | MS.gene45439:intron |
| TCTTTGCGGTGTTTCTTTGG+TGG | 0.480482 | 8.4:-78423383 | None:intergenic |
| TAGTGCACTCTTTAGAAGAA+TGG | 0.487581 | 8.4:+78424030 | MS.gene45439:CDS |
| AATTAATTACCTCACAAGAA+TGG | 0.493620 | 8.4:-78423460 | None:intergenic |
| CTTGGTCCATGTTCCTCACA+TGG | 0.494863 | 8.4:+78419402 | MS.gene45439:CDS |
| TCTATGGAGGGTTATACGTT+AGG | 0.498146 | 8.4:+78422295 | MS.gene45439:CDS |
| AGGTACCACTTAGATGTGTA+TGG | 0.499644 | 8.4:-78419356 | None:intergenic |
| GACTGAAGAGGAACAGTTGA+AGG | 0.502498 | 8.4:+78423322 | MS.gene45439:CDS |
| GGTTAAGTTTGTCATCAAAA+CGG | 0.506159 | 8.4:-78419457 | None:intergenic |
| GTTCTTAACAGCATTCCAAA+AGG | 0.506865 | 8.4:+78422375 | MS.gene45439:CDS |
| CATGCACTCATTACCCTCCT+TGG | 0.507555 | 8.4:-78423993 | None:intergenic |
| AAGATATCAAAGAGCCACTT+AGG | 0.507741 | 8.4:-78415487 | None:intergenic |
| ATGTTCCTCACATGGGTCTA+CGG | 0.514853 | 8.4:+78419410 | MS.gene45439:CDS |
| AATCCATGTTTCAGAGCTGA+AGG | 0.516850 | 8.4:-78424101 | None:intergenic |
| ACTTTGAGATTGTTCCTAAG+TGG | 0.519727 | 8.4:+78415473 | MS.gene45439:CDS |
| GCTTCAGTTATAGCAATGGT+TGG | 0.522297 | 8.4:+78415406 | None:intergenic |
| CCATAAATGTAGTCATAAAG+TGG | 0.523026 | 8.4:-78419188 | None:intergenic |
| TGGCCTTCAGCTCTGAAACA+TGG | 0.524801 | 8.4:+78424098 | MS.gene45439:CDS |
| AATACCCAAGTACAGTGTTC+AGG | 0.526910 | 8.4:+78419493 | MS.gene45439:CDS |
| CAAACTTAACCTTCAAACTT+GGG | 0.528180 | 8.4:+78419469 | MS.gene45439:CDS |
| TGACTACATTTATGGAACAA+TGG | 0.531921 | 8.4:+78419196 | MS.gene45439:CDS |
| AGTGGTACCTTTGGTTGATG+TGG | 0.534470 | 8.4:+78419369 | MS.gene45439:CDS |
| GAACAGTTGAAGGCACCAAA+AGG | 0.535528 | 8.4:+78423332 | MS.gene45439:CDS |
| GGCGTAAAAGTGGTGAGCTT+AGG | 0.537268 | 8.4:+78422243 | MS.gene45439:CDS |
| TCTGCATAGATTTGGTTAGT+GGG | 0.543907 | 8.4:+78423290 | MS.gene45439:intron |
| GGCTATCAACAAAATGATTG+AGG | 0.545166 | 8.4:+78422194 | MS.gene45439:CDS |
| TGAGTGCATGGCGCATAGCT+GGG | 0.552882 | 8.4:+78424006 | MS.gene45439:CDS |
| AATTGCAGAATTGGTTGCCA+AGG | 0.563122 | 8.4:+78423976 | MS.gene45439:intron |
| TGAGCATTGCAGGAGTGTAA+TGG | 0.565393 | 8.4:-78423415 | None:intergenic |
| ACATGAGATACTTGAGAGGA+GGG | 0.569020 | 8.4:-78415510 | None:intergenic |
| GCAGAATTGGTTGCCAAGGA+GGG | 0.569227 | 8.4:+78423980 | MS.gene45439:intron |
| ATTGTGCCAACAAGGAGTTC+AGG | 0.570563 | 8.4:+78422458 | MS.gene45439:CDS |
| TTGGTCCATGTTCCTCACAT+GGG | 0.572419 | 8.4:+78419403 | MS.gene45439:CDS |
| ATGGAGGGTTATACGTTAGG+AGG | 0.573787 | 8.4:+78422298 | MS.gene45439:CDS |
| TGATGTGGCCTGTTACAGCT+TGG | 0.576380 | 8.4:+78419384 | MS.gene45439:CDS |
| TTCTTTCAACAATGAATGTG+CGG | 0.579977 | 8.4:-78419433 | None:intergenic |
| AATGGTATAAGCAGTCTTTG+CGG | 0.581704 | 8.4:-78423397 | None:intergenic |
| TGCAGAATTGGTTGCCAAGG+AGG | 0.586466 | 8.4:+78423979 | MS.gene45439:intron |
| GAAGAGCTTAACATCTATGG+AGG | 0.591774 | 8.4:+78422282 | MS.gene45439:CDS |
| TACATGAGATACTTGAGAGG+AGG | 0.595316 | 8.4:-78415511 | None:intergenic |
| GCCTACACATTGTGCCAACA+AGG | 0.596015 | 8.4:+78422450 | MS.gene45439:CDS |
| CAAGGAGGGTAATGAGTGCA+TGG | 0.597972 | 8.4:+78423994 | MS.gene45439:CDS |
| GGGCTGTTAAATGTGTTAAG+TGG | 0.598463 | 8.4:-78419283 | None:intergenic |
| CTAAGCCATACACATCTAAG+TGG | 0.600593 | 8.4:+78419351 | MS.gene45439:CDS |
| TGTGTATGGCTTAGAAGCCA+AGG | 0.607034 | 8.4:-78419342 | None:intergenic |
| CTTAGGTCTAATGAATCAAG+AGG | 0.608099 | 8.4:+78422260 | MS.gene45439:CDS |
| TGAAAATGTCCATTCTTGTG+AGG | 0.618898 | 8.4:+78423451 | MS.gene45439:CDS |
| TATACCTGAACACTGTACTT+GGG | 0.622663 | 8.4:-78419497 | None:intergenic |
| TGACTCATGCAACTCATCTG+AGG | 0.626453 | 8.4:-78419225 | None:intergenic |
| CATTTATGGAACAATGGACA+AGG | 0.639140 | 8.4:+78419202 | MS.gene45439:CDS |
| GGGAGATGGATTGACTGAAG+AGG | 0.648147 | 8.4:+78423310 | MS.gene45439:CDS |
| GTAGACCCATGTGAGGAACA+TGG | 0.648969 | 8.4:-78419408 | None:intergenic |
| TAGATGATAAATGGATTCTG+GGG | 0.656241 | 8.4:-78419303 | None:intergenic |
| TAAGAACAACTGCAACAACA+AGG | 0.662736 | 8.4:-78422359 | None:intergenic |
| TGGCTACAATATGATTAACA+TGG | 0.669008 | 8.4:+78424067 | MS.gene45439:CDS |
| TCCTTGTTGGCACAATGTGT+AGG | 0.674010 | 8.4:-78422451 | None:intergenic |
| AAGAGCTTAACATCTATGGA+GGG | 0.678853 | 8.4:+78422283 | MS.gene45439:CDS |
| TAACAGGCCACATCAACCAA+AGG | 0.681750 | 8.4:-78419376 | None:intergenic |
| TGCGGCCGTAGACCCATGTG+AGG | 0.692422 | 8.4:-78419415 | None:intergenic |
| ACAACTCAAGTATTACTCAG+AGG | 0.714164 | 8.4:+78422399 | MS.gene45439:CDS |
| GTGTACATGAGATACTTGAG+AGG | 0.718071 | 8.4:-78415514 | None:intergenic |
| GAATGGAATGAACATGAGTG+TGG | 0.724613 | 8.4:+78424047 | MS.gene45439:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 78415421 | 78424136 | 78415421 | ID=MS.gene45439 |
| chr8.4 | mRNA | 78415421 | 78424136 | 78415421 | ID=MS.gene45439.t1;Parent=MS.gene45439 |
| chr8.4 | exon | 78415421 | 78415542 | 78415421 | ID=MS.gene45439.t1.exon1;Parent=MS.gene45439.t1 |
| chr8.4 | CDS | 78415421 | 78415542 | 78415421 | ID=cds.MS.gene45439.t1;Parent=MS.gene45439.t1 |
| chr8.4 | exon | 78419136 | 78419514 | 78419136 | ID=MS.gene45439.t1.exon2;Parent=MS.gene45439.t1 |
| chr8.4 | CDS | 78419136 | 78419514 | 78419136 | ID=cds.MS.gene45439.t1;Parent=MS.gene45439.t1 |
| chr8.4 | exon | 78422171 | 78422479 | 78422171 | ID=MS.gene45439.t1.exon3;Parent=MS.gene45439.t1 |
| chr8.4 | CDS | 78422171 | 78422479 | 78422171 | ID=cds.MS.gene45439.t1;Parent=MS.gene45439.t1 |
| chr8.4 | exon | 78422622 | 78422720 | 78422622 | ID=MS.gene45439.t1.exon4;Parent=MS.gene45439.t1 |
| chr8.4 | CDS | 78422622 | 78422720 | 78422622 | ID=cds.MS.gene45439.t1;Parent=MS.gene45439.t1 |
| chr8.4 | exon | 78423299 | 78423472 | 78423299 | ID=MS.gene45439.t1.exon5;Parent=MS.gene45439.t1 |
| chr8.4 | CDS | 78423299 | 78423472 | 78423299 | ID=cds.MS.gene45439.t1;Parent=MS.gene45439.t1 |
| chr8.4 | exon | 78423984 | 78424136 | 78423984 | ID=MS.gene45439.t1.exon6;Parent=MS.gene45439.t1 |
| chr8.4 | CDS | 78423984 | 78424136 | 78423984 | ID=cds.MS.gene45439.t1;Parent=MS.gene45439.t1 |
| Gene Sequence |
| Protein sequence |
