Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061113.t1 | RHN64373.1 | 93.9 | 33 | 2 | 0 | 1 | 33 | 1 | 33 | 7.70E-07 | 63.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061113.t1 | G7JNX3 | 93.9 | 33 | 2 | 0 | 1 | 33 | 1 | 33 | 5.6e-07 | 63.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049758 | MS.gene061113 | 0.818254 | 2.16E-52 | -1.69E-46 |
| MS.gene049915 | MS.gene061113 | 0.80347 | 3.43E-49 | -1.69E-46 |
| MS.gene051662 | MS.gene061113 | 0.819026 | 1.44E-52 | -1.69E-46 |
| MS.gene051663 | MS.gene061113 | 0.802099 | 6.59E-49 | -1.69E-46 |
| MS.gene052178 | MS.gene061113 | 0.805164 | 1.52E-49 | -1.69E-46 |
| MS.gene052302 | MS.gene061113 | 0.811069 | 8.42E-51 | -1.69E-46 |
| MS.gene052376 | MS.gene061113 | 0.815394 | 9.46E-52 | -1.69E-46 |
| MS.gene053630 | MS.gene061113 | 0.809323 | 2.00E-50 | -1.69E-46 |
| MS.gene053836 | MS.gene061113 | 0.816881 | 4.40E-52 | -1.69E-46 |
| MS.gene055133 | MS.gene061113 | 0.816549 | 5.22E-52 | -1.69E-46 |
| MS.gene055207 | MS.gene061113 | 0.809943 | 1.47E-50 | -1.69E-46 |
| MS.gene057754 | MS.gene061113 | 0.847649 | 1.01E-59 | -1.69E-46 |
| MS.gene058201 | MS.gene061113 | 0.802453 | 5.57E-49 | -1.69E-46 |
| MS.gene058749 | MS.gene061113 | 0.817077 | 3.98E-52 | -1.69E-46 |
| MS.gene059228 | MS.gene061113 | 0.803906 | 2.79E-49 | -1.69E-46 |
| MS.gene059894 | MS.gene061113 | 0.825192 | 5.36E-54 | -1.69E-46 |
| MS.gene060476 | MS.gene061113 | 0.82937 | 5.35E-55 | -1.69E-46 |
| MS.gene061049 | MS.gene061113 | 0.80048 | 1.41E-48 | -1.69E-46 |
| MS.gene061112 | MS.gene061113 | 0.888724 | 4.58E-73 | -1.69E-46 |
| MS.gene061113 | MS.gene061265 | 0.883066 | 6.14E-71 | -1.69E-46 |
| MS.gene061113 | MS.gene061788 | 0.8005 | 1.40E-48 | -1.69E-46 |
| MS.gene061113 | MS.gene062356 | 0.803484 | 3.41E-49 | -1.69E-46 |
| MS.gene061113 | MS.gene062978 | 0.840691 | 7.46E-58 | -1.69E-46 |
| MS.gene061113 | MS.gene063103 | 0.805005 | 1.64E-49 | -1.69E-46 |
| MS.gene061113 | MS.gene063544 | 0.804518 | 2.08E-49 | -1.69E-46 |
| MS.gene061113 | MS.gene06367 | 0.805286 | 1.44E-49 | -1.69E-46 |
| MS.gene061113 | MS.gene064679 | 0.809671 | 1.69E-50 | -1.69E-46 |
| MS.gene061113 | MS.gene065133 | 0.823983 | 1.03E-53 | -1.69E-46 |
| MS.gene061113 | MS.gene065137 | 0.824418 | 8.16E-54 | -1.69E-46 |
| MS.gene061113 | MS.gene065138 | 0.814555 | 1.45E-51 | -1.69E-46 |
| MS.gene061113 | MS.gene065378 | 0.855037 | 8.26E-62 | -1.69E-46 |
| MS.gene061113 | MS.gene065581 | 0.805403 | 1.36E-49 | -1.69E-46 |
| MS.gene061113 | MS.gene066019 | 0.834001 | 3.86E-56 | -1.69E-46 |
| MS.gene061113 | MS.gene066856 | 0.809831 | 1.56E-50 | -1.69E-46 |
| MS.gene061113 | MS.gene067029 | 0.802648 | 5.08E-49 | -1.69E-46 |
| MS.gene061113 | MS.gene06806 | 0.800503 | 1.40E-48 | -1.69E-46 |
| MS.gene061113 | MS.gene068614 | 0.809112 | 2.22E-50 | -1.69E-46 |
| MS.gene061113 | MS.gene069147 | 0.809281 | 2.05E-50 | -1.69E-46 |
| MS.gene061113 | MS.gene069559 | 0.813613 | 2.34E-51 | -1.69E-46 |
| MS.gene061113 | MS.gene069679 | 0.832214 | 1.07E-55 | -1.69E-46 |
| MS.gene061113 | MS.gene069680 | 0.807687 | 4.48E-50 | -1.69E-46 |
| MS.gene061113 | MS.gene069681 | 0.809943 | 1.47E-50 | -1.69E-46 |
| MS.gene061113 | MS.gene069682 | 0.80526 | 1.45E-49 | -1.69E-46 |
| MS.gene061113 | MS.gene070876 | 0.813502 | 2.48E-51 | -1.69E-46 |
| MS.gene061113 | MS.gene070898 | 0.812739 | 3.64E-51 | -1.69E-46 |
| MS.gene061113 | MS.gene072732 | 0.802543 | 5.34E-49 | -1.69E-46 |
| MS.gene061113 | MS.gene073309 | 0.803171 | 3.96E-49 | -1.69E-46 |
| MS.gene061113 | MS.gene073696 | 0.806754 | 7.05E-50 | -1.69E-46 |
| MS.gene061113 | MS.gene073926 | 0.823864 | 1.10E-53 | -1.69E-46 |
| MS.gene061113 | MS.gene073962 | 0.827124 | 1.86E-54 | -1.69E-46 |
| MS.gene061113 | MS.gene07407 | 0.820067 | 8.34E-53 | -1.69E-46 |
| MS.gene061113 | MS.gene074091 | 0.814232 | 1.71E-51 | -1.69E-46 |
| MS.gene061113 | MS.gene08514 | 0.823554 | 1.30E-53 | -1.69E-46 |
| MS.gene061113 | MS.gene09136 | 0.805621 | 1.22E-49 | -1.69E-46 |
| MS.gene061113 | MS.gene20438 | 0.812358 | 4.41E-51 | -1.69E-46 |
| MS.gene061113 | MS.gene21659 | 0.814113 | 1.82E-51 | -1.69E-46 |
| MS.gene061113 | MS.gene22314 | 0.800868 | 1.18E-48 | -1.69E-46 |
| MS.gene061113 | MS.gene23088 | 0.801864 | 7.36E-49 | -1.69E-46 |
| MS.gene061113 | MS.gene23251 | 0.807117 | 5.91E-50 | -1.69E-46 |
| MS.gene061113 | MS.gene23378 | 0.802706 | 4.94E-49 | -1.69E-46 |
| MS.gene061113 | MS.gene23887 | 0.830992 | 2.15E-55 | -1.69E-46 |
| MS.gene061113 | MS.gene24039 | 0.81598 | 7.00E-52 | -1.69E-46 |
| MS.gene061113 | MS.gene24118 | 0.809908 | 1.50E-50 | -1.69E-46 |
| MS.gene061113 | MS.gene24546 | 0.800487 | 1.41E-48 | -1.69E-46 |
| MS.gene061113 | MS.gene25229 | 0.801178 | 1.02E-48 | -1.69E-46 |
| MS.gene061113 | MS.gene26990 | 0.813654 | 2.29E-51 | -1.69E-46 |
| MS.gene061113 | MS.gene27095 | 0.823671 | 1.22E-53 | -1.69E-46 |
| MS.gene061113 | MS.gene27217 | 0.819862 | 9.29E-53 | -1.69E-46 |
| MS.gene061113 | MS.gene27393 | 0.811041 | 8.54E-51 | -1.69E-46 |
| MS.gene061113 | MS.gene28929 | 0.800556 | 1.36E-48 | -1.69E-46 |
| MS.gene061113 | MS.gene29820 | 0.849269 | 3.60E-60 | -1.69E-46 |
| MS.gene061113 | MS.gene33248 | 0.816354 | 5.77E-52 | -1.69E-46 |
| MS.gene061113 | MS.gene35590 | 0.845369 | 4.24E-59 | -1.69E-46 |
| MS.gene061113 | MS.gene38758 | 0.813934 | 1.99E-51 | -1.69E-46 |
| MS.gene061113 | MS.gene39038 | 0.813233 | 2.84E-51 | -1.69E-46 |
| MS.gene061113 | MS.gene39341 | 0.836385 | 9.65E-57 | -1.69E-46 |
| MS.gene061113 | MS.gene39658 | 0.847331 | 1.24E-59 | -1.69E-46 |
| MS.gene061113 | MS.gene40106 | 0.836441 | 9.34E-57 | -1.69E-46 |
| MS.gene061113 | MS.gene43954 | 0.821789 | 3.35E-53 | -1.69E-46 |
| MS.gene061113 | MS.gene44007 | 0.808266 | 3.37E-50 | -1.69E-46 |
| MS.gene061113 | MS.gene45085 | 0.822115 | 2.81E-53 | -1.69E-46 |
| MS.gene061113 | MS.gene45220 | 0.806112 | 9.63E-50 | -1.69E-46 |
| MS.gene061113 | MS.gene45439 | 0.81496 | 1.18E-51 | -1.69E-46 |
| MS.gene061113 | MS.gene46110 | 0.807465 | 4.99E-50 | -1.69E-46 |
| MS.gene061113 | MS.gene46176 | 0.827398 | 1.60E-54 | -1.69E-46 |
| MS.gene061113 | MS.gene47146 | 0.814656 | 1.38E-51 | -1.69E-46 |
| MS.gene061113 | MS.gene47283 | 0.803075 | 4.14E-49 | -1.69E-46 |
| MS.gene061113 | MS.gene48195 | 0.805569 | 1.25E-49 | -1.69E-46 |
| MS.gene061113 | MS.gene48796 | 0.814584 | 1.43E-51 | -1.69E-46 |
| MS.gene061113 | MS.gene50217 | 0.808543 | 2.94E-50 | -1.69E-46 |
| MS.gene061113 | MS.gene50827 | 0.801243 | 9.87E-49 | -1.69E-46 |
| MS.gene061113 | MS.gene52198 | 0.805727 | 1.16E-49 | -1.69E-46 |
| MS.gene061113 | MS.gene53056 | 0.837737 | 4.36E-57 | -1.69E-46 |
| MS.gene061113 | MS.gene53556 | 0.822439 | 2.37E-53 | -1.69E-46 |
| MS.gene061113 | MS.gene54221 | 0.808798 | 2.59E-50 | -1.69E-46 |
| MS.gene061113 | MS.gene54407 | 0.81431 | 1.64E-51 | -1.69E-46 |
| MS.gene061113 | MS.gene54408 | 0.806651 | 7.42E-50 | -1.69E-46 |
| MS.gene061113 | MS.gene54700 | 0.8149 | 1.22E-51 | -1.69E-46 |
| MS.gene061113 | MS.gene55263 | 0.843134 | 1.69E-58 | -1.69E-46 |
| MS.gene061113 | MS.gene55505 | 0.831522 | 1.59E-55 | -1.69E-46 |
| MS.gene061113 | MS.gene57222 | 0.806829 | 6.80E-50 | -1.69E-46 |
| MS.gene061113 | MS.gene57299 | 0.812767 | 3.59E-51 | -1.69E-46 |
| MS.gene061113 | MS.gene59035 | 0.810872 | 9.29E-51 | -1.69E-46 |
| MS.gene061113 | MS.gene59570 | 0.80717 | 5.76E-50 | -1.69E-46 |
| MS.gene061113 | MS.gene61582 | 0.819379 | 1.20E-52 | -1.69E-46 |
| MS.gene061113 | MS.gene61586 | 0.858525 | 7.76E-63 | -1.69E-46 |
| MS.gene061113 | MS.gene62720 | 0.83939 | 1.63E-57 | -1.69E-46 |
| MS.gene061113 | MS.gene63009 | 0.807753 | 4.33E-50 | -1.69E-46 |
| MS.gene061113 | MS.gene64424 | 0.822889 | 1.86E-53 | -1.69E-46 |
| MS.gene061113 | MS.gene64426 | 0.80705 | 6.11E-50 | -1.69E-46 |
| MS.gene061113 | MS.gene64621 | 0.812722 | 3.67E-51 | -1.69E-46 |
| MS.gene061113 | MS.gene65851 | 0.807295 | 5.42E-50 | -1.69E-46 |
| MS.gene061113 | MS.gene66292 | 0.802511 | 5.42E-49 | -1.69E-46 |
| MS.gene061113 | MS.gene66884 | 0.805002 | 1.65E-49 | -1.69E-46 |
| MS.gene061113 | MS.gene68151 | 0.820218 | 7.71E-53 | -1.69E-46 |
| MS.gene061113 | MS.gene68393 | 0.823045 | 1.71E-53 | -1.69E-46 |
| MS.gene061113 | MS.gene68751 | 0.808331 | 3.26E-50 | -1.69E-46 |
| MS.gene061113 | MS.gene68877 | 0.810509 | 1.11E-50 | -1.69E-46 |
| MS.gene061113 | MS.gene68959 | 0.801647 | 8.16E-49 | -1.69E-46 |
| MS.gene061113 | MS.gene69083 | 0.807268 | 5.49E-50 | -1.69E-46 |
| MS.gene061113 | MS.gene69314 | 0.810299 | 1.24E-50 | -1.69E-46 |
| MS.gene061113 | MS.gene70337 | 0.81418 | 1.76E-51 | -1.69E-46 |
| MS.gene061113 | MS.gene70819 | 0.819601 | 1.07E-52 | -1.69E-46 |
| MS.gene061113 | MS.gene71024 | 0.820553 | 6.46E-53 | -1.69E-46 |
| MS.gene061113 | MS.gene71439 | 0.818399 | 2.00E-52 | -1.69E-46 |
| MS.gene061113 | MS.gene71551 | 0.827975 | 1.16E-54 | -1.69E-46 |
| MS.gene061113 | MS.gene71604 | 0.819242 | 1.29E-52 | -1.69E-46 |
| MS.gene061113 | MS.gene71833 | 0.814283 | 1.67E-51 | -1.69E-46 |
| MS.gene061113 | MS.gene73227 | 0.806624 | 7.51E-50 | -1.69E-46 |
| MS.gene061113 | MS.gene73268 | 0.802922 | 4.46E-49 | -1.69E-46 |
| MS.gene061113 | MS.gene74443 | 0.831751 | 1.40E-55 | -1.69E-46 |
| MS.gene061113 | MS.gene74922 | 0.81773 | 2.83E-52 | -1.69E-46 |
| MS.gene061113 | MS.gene74960 | 0.802981 | 4.33E-49 | -1.69E-46 |
| MS.gene061113 | MS.gene75021 | 0.810762 | 9.81E-51 | -1.69E-46 |
| MS.gene061113 | MS.gene75128 | 0.828368 | 9.35E-55 | -1.69E-46 |
| MS.gene061113 | MS.gene76620 | 0.840734 | 7.28E-58 | -1.69E-46 |
| MS.gene061113 | MS.gene78925 | 0.822537 | 2.25E-53 | -1.69E-46 |
| MS.gene061113 | MS.gene78973 | 0.805189 | 1.50E-49 | -1.69E-46 |
| MS.gene061113 | MS.gene80283 | 0.807691 | 4.47E-50 | -1.69E-46 |
| MS.gene061113 | MS.gene80996 | 0.813904 | 2.02E-51 | -1.69E-46 |
| MS.gene061113 | MS.gene80997 | 0.806785 | 6.95E-50 | -1.69E-46 |
| MS.gene061113 | MS.gene81421 | 0.818896 | 1.54E-52 | -1.69E-46 |
| MS.gene061113 | MS.gene81680 | 0.826405 | 2.76E-54 | -1.69E-46 |
| MS.gene061113 | MS.gene81687 | 0.833499 | 5.15E-56 | -1.69E-46 |
| MS.gene061113 | MS.gene81689 | 0.854319 | 1.33E-61 | -1.69E-46 |
| MS.gene061113 | MS.gene82256 | 0.802197 | 6.29E-49 | -1.69E-46 |
| MS.gene061113 | MS.gene82811 | 0.806635 | 7.48E-50 | -1.69E-46 |
| MS.gene061113 | MS.gene84086 | 0.811701 | 6.14E-51 | -1.69E-46 |
| MS.gene061113 | MS.gene85168 | 0.806248 | 9.02E-50 | -1.69E-46 |
| MS.gene061113 | MS.gene85943 | 0.805354 | 1.39E-49 | -1.69E-46 |
| MS.gene061113 | MS.gene88960 | 0.80279 | 4.75E-49 | -1.69E-46 |
| MS.gene061113 | MS.gene89238 | 0.801981 | 6.97E-49 | -1.69E-46 |
| MS.gene061113 | MS.gene89326 | 0.827106 | 1.88E-54 | -1.69E-46 |
| MS.gene061113 | MS.gene91548 | 0.803165 | 3.97E-49 | -1.69E-46 |
| MS.gene061113 | MS.gene92548 | 0.809441 | 1.89E-50 | -1.69E-46 |
| MS.gene061113 | MS.gene93890 | 0.803355 | 3.63E-49 | -1.69E-46 |
| MS.gene061113 | MS.gene94149 | 0.821732 | 3.45E-53 | -1.69E-46 |
| MS.gene061113 | MS.gene94150 | 0.835974 | 1.23E-56 | -1.69E-46 |
| MS.gene061113 | MS.gene94151 | 0.800799 | 1.22E-48 | -1.69E-46 |
| MS.gene061113 | MS.gene95283 | 0.817564 | 3.09E-52 | -1.69E-46 |
| MS.gene061113 | MS.gene96220 | 0.833236 | 5.99E-56 | -1.69E-46 |
| MS.gene061113 | MS.gene96284 | 0.810516 | 1.11E-50 | -1.69E-46 |
| MS.gene061113 | MS.gene96705 | 0.81569 | 8.12E-52 | -1.69E-46 |
| MS.gene061113 | MS.gene97858 | 0.801283 | 9.69E-49 | -1.69E-46 |
| MS.gene061113 | MS.gene98316 | 0.832039 | 1.19E-55 | -1.69E-46 |
| MS.gene061113 | MS.gene98521 | 0.8153 | 9.92E-52 | -1.69E-46 |
| MS.gene061113 | MS.gene98522 | 0.822102 | 2.83E-53 | -1.69E-46 |
| MS.gene061113 | MS.gene98858 | 0.804895 | 1.73E-49 | -1.69E-46 |
| MS.gene061113 | MS.gene99735 | 0.808016 | 3.81E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061113.t1 | MTR_4g122180 | 91.489 | 47 | 4 | 0 | 1 | 47 | 1 | 47 | 6.82e-15 | 68.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 61 sgRNAs with CRISPR-Local
Find 74 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGTGGTGCCGGCGGAGGTTT+TGG | 0.242299 | 8.3:+6662688 | MS.gene061113:CDS |
| GTGGACATGGTGGTGGAATC+GGG | 0.282878 | 8.3:+6662892 | MS.gene061113:CDS |
| GGTGGCTTTGGAGGCGGTGC+TGG | 0.301658 | 8.3:+6662754 | MS.gene061113:CDS |
| CTTGGACACGGTGGTGGCTT+TGG | 0.308545 | 8.3:+6662742 | MS.gene061113:CDS |
| GGTGGACATGGTGGTGGAAT+CGG | 0.315986 | 8.3:+6662891 | MS.gene061113:CDS |
| TGGTGGTGCAGGTGTGGAAT+TGG | 0.325614 | 8.3:+6662867 | MS.gene061113:CDS |
| GGTGGTGCAGGTGGAGGCTA+TGG | 0.345394 | 8.3:+6662778 | MS.gene061113:CDS |
| CTAGGTGGAGGTGGTGGTGC+AGG | 0.346632 | 8.3:+6662856 | MS.gene061113:CDS |
| GGTGGAAGCGGTGGTGGTAT+AGG | 0.348764 | 8.3:+6662915 | MS.gene061113:CDS |
| GGTGGTCATGGTGGTGGACT+AGG | 0.370304 | 8.3:+6662838 | MS.gene061113:CDS |
| GGCGGTGCTGGAGGTGGTGC+AGG | 0.379929 | 8.3:+6662766 | MS.gene061113:CDS |
| GTGATATTTGCATTGGTGAT+AGG | 0.388416 | 8.3:+6662598 | MS.gene061113:CDS |
| GGTGGCGTGGGTGGTGGTGC+CGG | 0.391358 | 8.3:+6662676 | MS.gene061113:CDS |
| GGCGGCAGTGGTGGAGGTGT+TGG | 0.392236 | 8.3:+6662712 | MS.gene061113:CDS |
| GGAGGTGTTGGAGGAGGACT+TGG | 0.400513 | 8.3:+6662724 | MS.gene061113:CDS |
| TGGAGGTGGTGGTGCAGGTG+TGG | 0.406412 | 8.3:+6662861 | MS.gene061113:CDS |
| TGGTGCAGGTGTGGAATTGG+AGG | 0.408798 | 8.3:+6662870 | MS.gene061113:CDS |
| TGGACATGGTGGTGGAATCG+GGG | 0.412271 | 8.3:+6662893 | MS.gene061113:CDS |
| GGTGGAGGTGGCGTGGGTGG+TGG | 0.413145 | 8.3:+6662670 | MS.gene061113:CDS |
| GCTGGAGGTGGTGCAGGTGG+AGG | 0.414524 | 8.3:+6662772 | MS.gene061113:CDS |
| AACTTTGGTGGAGGTGGCGT+GGG | 0.417311 | 8.3:+6662664 | MS.gene061113:CDS |
| TTTGGTGGCGGCAGTGGTGG+AGG | 0.430606 | 8.3:+6662706 | MS.gene061113:CDS |
| TTTGGAGGCGGTGCTGGAGG+TGG | 0.431384 | 8.3:+6662760 | MS.gene061113:CDS |
| ATTGGAGGTGGACATGGTGG+TGG | 0.437569 | 8.3:+6662885 | MS.gene061113:CDS |
| GGTGGACTAGGTGGAGGTGG+TGG | 0.441020 | 8.3:+6662850 | MS.gene061113:CDS |
| GTGGGTGGTGGTGCCGGCGG+AGG | 0.443554 | 8.3:+6662682 | MS.gene061113:CDS |
| GGACACGGTGGTGGCTTTGG+AGG | 0.444375 | 8.3:+6662745 | MS.gene061113:CDS |
| GATGAATTTGTTGACAACTT+TGG | 0.444899 | 8.3:+6662649 | MS.gene061113:CDS |
| CACGGTGGTGGCTTTGGAGG+CGG | 0.457544 | 8.3:+6662748 | MS.gene061113:CDS |
| GGAGGAATTGGAGGTGGTCA+TGG | 0.457657 | 8.3:+6662826 | MS.gene061113:CDS |
| CTATGGAGGTGGTAAGGTGG+TGG | 0.459799 | 8.3:+6662795 | MS.gene061113:CDS |
| ATTGGAGGTGGTCATGGTGG+TGG | 0.460184 | 8.3:+6662832 | MS.gene061113:CDS |
| GGACATGGTGGTGGAATCGG+GGG | 0.464354 | 8.3:+6662894 | MS.gene061113:CDS |
| AGTGGTGGAGGTGTTGGAGG+AGG | 0.467091 | 8.3:+6662718 | MS.gene061113:CDS |
| GGTGGTGGACTAGGTGGAGG+TGG | 0.467193 | 8.3:+6662847 | MS.gene061113:CDS |
| GGAGGACTTGGACACGGTGG+TGG | 0.468197 | 8.3:+6662736 | MS.gene061113:CDS |
| GGTGCAGGTGGAGGCTATGG+AGG | 0.476723 | 8.3:+6662781 | MS.gene061113:CDS |
| TTTGGTGGAGGTGGCGTGGG+TGG | 0.489746 | 8.3:+6662667 | MS.gene061113:CDS |
| GGCGTGGGTGGTGGTGCCGG+CGG | 0.491147 | 8.3:+6662679 | MS.gene061113:CDS |
| GGCTTTGGAGGCGGTGCTGG+AGG | 0.492028 | 8.3:+6662757 | MS.gene061113:CDS |
| GGCAGTGGTGGAGGTGTTGG+AGG | 0.492172 | 8.3:+6662715 | MS.gene061113:CDS |
| CAACTTTGGTGGAGGTGGCG+TGG | 0.494540 | 8.3:+6662663 | MS.gene061113:CDS |
| AGGCTATGGAGGTGGTAAGG+TGG | 0.502719 | 8.3:+6662792 | MS.gene061113:CDS |
| GGAATTGGAGGTGGACATGG+TGG | 0.503820 | 8.3:+6662882 | MS.gene061113:CDS |
| TTTGGAGGAGGAATTGGAGG+TGG | 0.514889 | 8.3:+6662820 | MS.gene061113:CDS |
| GTTGACAACTTTGGTGGAGG+TGG | 0.516532 | 8.3:+6662658 | MS.gene061113:CDS |
| TGGAGGCTATGGAGGTGGTA+AGG | 0.521084 | 8.3:+6662789 | MS.gene061113:CDS |
| TGCAGGTGTGGAATTGGAGG+TGG | 0.522485 | 8.3:+6662873 | MS.gene061113:CDS |
| TGTGGAATTGGAGGTGGACA+TGG | 0.525026 | 8.3:+6662879 | MS.gene061113:CDS |
| CATGGTGGTGGACTAGGTGG+AGG | 0.527570 | 8.3:+6662844 | MS.gene061113:CDS |
| GCAGGTGGAGGCTATGGAGG+TGG | 0.536135 | 8.3:+6662784 | MS.gene061113:CDS |
| GGTCATGGTGGTGGACTAGG+TGG | 0.544222 | 8.3:+6662841 | MS.gene061113:CDS |
| GGTGCTGGAGGTGGTGCAGG+TGG | 0.552417 | 8.3:+6662769 | MS.gene061113:CDS |
| GAATTTGTTGACAACTTTGG+TGG | 0.555943 | 8.3:+6662652 | MS.gene061113:CDS |
| GGAATTGGAGGTGGTCATGG+TGG | 0.557632 | 8.3:+6662829 | MS.gene061113:CDS |
| ACTTGCTGTGATATTTGCAT+TGG | 0.561478 | 8.3:+6662591 | MS.gene061113:CDS |
| GCCGCCACCAAAACCTCCGC+CGG | 0.570676 | 8.3:-6662695 | None:intergenic |
| TTTGTTGACAACTTTGGTGG+AGG | 0.589971 | 8.3:+6662655 | MS.gene061113:CDS |
| TGATAGGAACATCTGAATGC+CGG | 0.590146 | 8.3:+6662614 | MS.gene061113:CDS |
| GTTGGAGGAGGACTTGGACA+CGG | 0.664809 | 8.3:+6662730 | MS.gene061113:CDS |
| GGAGGAGGACTTGGACACGG+TGG | 0.721731 | 8.3:+6662733 | MS.gene061113:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTCATCTTTTTGTATTTTC+CGG | - | chr8.3:6662636-6662655 | None:intergenic | 20.0% |
| !! | GATGAATTTGTTGACAACTT+TGG | + | chr8.3:6662649-6662668 | MS.gene061113:CDS | 30.0% |
| ! | GTGATATTTGCATTGGTGAT+AGG | + | chr8.3:6662598-6662617 | MS.gene061113:CDS | 35.0% |
| !! | ACTTGCTGTGATATTTGCAT+TGG | + | chr8.3:6662591-6662610 | MS.gene061113:CDS | 35.0% |
| !! | GAATTTGTTGACAACTTTGG+TGG | + | chr8.3:6662652-6662671 | MS.gene061113:CDS | 35.0% |
| TGATAGGAACATCTGAATGC+CGG | + | chr8.3:6662614-6662633 | MS.gene061113:CDS | 40.0% | |
| TTTGTTGACAACTTTGGTGG+AGG | + | chr8.3:6662655-6662674 | MS.gene061113:CDS | 40.0% | |
| !! | GTGGTTTTTGGAGGAGGAAT+TGG | + | chr8.3:6662814-6662833 | MS.gene061113:CDS | 45.0% |
| !! | GTTTTTGGAGGAGGAATTGG+AGG | + | chr8.3:6662817-6662836 | MS.gene061113:CDS | 45.0% |
| GTTGACAACTTTGGTGGAGG+TGG | + | chr8.3:6662658-6662677 | MS.gene061113:CDS | 50.0% | |
| TGTGGAATTGGAGGTGGACA+TGG | + | chr8.3:6662879-6662898 | MS.gene061113:CDS | 50.0% | |
| TTTGGAGGAGGAATTGGAGG+TGG | + | chr8.3:6662820-6662839 | MS.gene061113:CDS | 50.0% | |
| !! | AAGGTGGTGGTTTTTGGAGG+AGG | + | chr8.3:6662808-6662827 | MS.gene061113:CDS | 50.0% |
| !! | GGTAAGGTGGTGGTTTTTGG+AGG | + | chr8.3:6662805-6662824 | MS.gene061113:CDS | 50.0% |
| !! | GGTGGTAAGGTGGTGGTTTT+TGG | + | chr8.3:6662802-6662821 | MS.gene061113:CDS | 50.0% |
| AACTTTGGTGGAGGTGGCGT+GGG | + | chr8.3:6662664-6662683 | MS.gene061113:CDS | 55.0% | |
| AGGCTATGGAGGTGGTAAGG+TGG | + | chr8.3:6662792-6662811 | MS.gene061113:CDS | 55.0% | |
| ATTGGAGGTGGACATGGTGG+TGG | + | chr8.3:6662885-6662904 | MS.gene061113:CDS | 55.0% | |
| ATTGGAGGTGGTCATGGTGG+TGG | + | chr8.3:6662832-6662851 | MS.gene061113:CDS | 55.0% | |
| CTATGGAGGTGGTAAGGTGG+TGG | + | chr8.3:6662795-6662814 | MS.gene061113:CDS | 55.0% | |
| GGAATTGGAGGTGGACATGG+TGG | + | chr8.3:6662882-6662901 | MS.gene061113:CDS | 55.0% | |
| GGAATTGGAGGTGGTCATGG+TGG | + | chr8.3:6662829-6662848 | MS.gene061113:CDS | 55.0% | |
| GGAGGAATTGGAGGTGGTCA+TGG | + | chr8.3:6662826-6662845 | MS.gene061113:CDS | 55.0% | |
| GTTGGAGGAGGACTTGGACA+CGG | + | chr8.3:6662730-6662749 | MS.gene061113:CDS | 55.0% | |
| TGCAGGTGTGGAATTGGAGG+TGG | + | chr8.3:6662873-6662892 | MS.gene061113:CDS | 55.0% | |
| TGGAGGCTATGGAGGTGGTA+AGG | + | chr8.3:6662789-6662808 | MS.gene061113:CDS | 55.0% | |
| TGGTGCAGGTGTGGAATTGG+AGG | + | chr8.3:6662870-6662889 | MS.gene061113:CDS | 55.0% | |
| ! | TGGTGGTGCAGGTGTGGAAT+TGG | + | chr8.3:6662867-6662886 | MS.gene061113:CDS | 55.0% |
| !! | GGTGGACATGGTGGTGGAAT+CGG | + | chr8.3:6662891-6662910 | MS.gene061113:CDS | 55.0% |
| !! | GTGGACATGGTGGTGGAATC+GGG | + | chr8.3:6662892-6662911 | MS.gene061113:CDS | 55.0% |
| !! | TGGACATGGTGGTGGAATCG+GGG | + | chr8.3:6662893-6662912 | MS.gene061113:CDS | 55.0% |
| CAACTTTGGTGGAGGTGGCG+TGG | + | chr8.3:6662663-6662682 | MS.gene061113:CDS | 60.0% | |
| ! | CTTGGACACGGTGGTGGCTT+TGG | + | chr8.3:6662742-6662761 | MS.gene061113:CDS | 60.0% |
| !! | AGTGGTGGAGGTGTTGGAGG+AGG | + | chr8.3:6662718-6662737 | MS.gene061113:CDS | 60.0% |
| !! | CATGGTGGTGGACTAGGTGG+AGG | + | chr8.3:6662844-6662863 | MS.gene061113:CDS | 60.0% |
| !! | GGACATGGTGGTGGAATCGG+GGG | + | chr8.3:6662894-6662913 | MS.gene061113:CDS | 60.0% |
| !! | GGAGGTGTTGGAGGAGGACT+TGG | + | chr8.3:6662724-6662743 | MS.gene061113:CDS | 60.0% |
| !! | GGTCATGGTGGTGGACTAGG+TGG | + | chr8.3:6662841-6662860 | MS.gene061113:CDS | 60.0% |
| !! | GGTGGTCATGGTGGTGGACT+AGG | + | chr8.3:6662838-6662857 | MS.gene061113:CDS | 60.0% |
| GCAGGTGGAGGCTATGGAGG+TGG | + | chr8.3:6662784-6662803 | MS.gene061113:CDS | 65.0% | |
| GGAGGACTTGGACACGGTGG+TGG | + | chr8.3:6662736-6662755 | MS.gene061113:CDS | 65.0% | |
| GGAGGAGGACTTGGACACGG+TGG | + | chr8.3:6662733-6662752 | MS.gene061113:CDS | 65.0% | |
| GGTGCAGGTGGAGGCTATGG+AGG | + | chr8.3:6662781-6662800 | MS.gene061113:CDS | 65.0% | |
| GGTGGAATCGGGGGTGGAAG+CGG | + | chr8.3:6662903-6662922 | MS.gene061113:CDS | 65.0% | |
| GGTGGTGCAGGTGGAGGCTA+TGG | + | chr8.3:6662778-6662797 | MS.gene061113:CDS | 65.0% | |
| TTTGGTGGAGGTGGCGTGGG+TGG | + | chr8.3:6662667-6662686 | MS.gene061113:CDS | 65.0% | |
| ! | CTAGGTGGAGGTGGTGGTGC+AGG | + | chr8.3:6662856-6662875 | MS.gene061113:CDS | 65.0% |
| ! | TGGAGGTGGTGGTGCAGGTG+TGG | + | chr8.3:6662861-6662880 | MS.gene061113:CDS | 65.0% |
| ! | TTTGGAGGCGGTGCTGGAGG+TGG | + | chr8.3:6662760-6662779 | MS.gene061113:CDS | 65.0% |
| !! | CACGGTGGTGGCTTTGGAGG+CGG | + | chr8.3:6662748-6662767 | MS.gene061113:CDS | 65.0% |
| !! | CATGGTGGTGGAATCGGGGG+TGG | + | chr8.3:6662897-6662916 | MS.gene061113:CDS | 65.0% |
| !! | GGACACGGTGGTGGCTTTGG+AGG | + | chr8.3:6662745-6662764 | MS.gene061113:CDS | 65.0% |
| !! | GGCAGTGGTGGAGGTGTTGG+AGG | + | chr8.3:6662715-6662734 | MS.gene061113:CDS | 65.0% |
| !! | GGTGGACTAGGTGGAGGTGG+TGG | + | chr8.3:6662850-6662869 | MS.gene061113:CDS | 65.0% |
| !! | GGTGGTGGACTAGGTGGAGG+TGG | + | chr8.3:6662847-6662866 | MS.gene061113:CDS | 65.0% |
| !! | TTTGGTGGCGGCAGTGGTGG+AGG | + | chr8.3:6662706-6662725 | MS.gene061113:CDS | 65.0% |
| !!! | GGAGGTTTTGGTGGCGGCAG+TGG | + | chr8.3:6662700-6662719 | MS.gene061113:CDS | 65.0% |
| !!! | GGTTTTGGTGGCGGCAGTGG+TGG | + | chr8.3:6662703-6662722 | MS.gene061113:CDS | 65.0% |
| ATCGGGGGTGGAAGCGGTGG+TGG | + | chr8.3:6662909-6662928 | MS.gene061113:CDS | 70.0% | |
| GCCGCCACCAAAACCTCCGC+CGG | - | chr8.3:6662698-6662717 | None:intergenic | 70.0% | |
| GCTGGAGGTGGTGCAGGTGG+AGG | + | chr8.3:6662772-6662791 | MS.gene061113:CDS | 70.0% | |
| GGAATCGGGGGTGGAAGCGG+TGG | + | chr8.3:6662906-6662925 | MS.gene061113:CDS | 70.0% | |
| ! | GGCGGCAGTGGTGGAGGTGT+TGG | + | chr8.3:6662712-6662731 | MS.gene061113:CDS | 70.0% |
| ! | GGTGCTGGAGGTGGTGCAGG+TGG | + | chr8.3:6662769-6662788 | MS.gene061113:CDS | 70.0% |
| !! | GCCGGCGGAGGTTTTGGTGG+CGG | + | chr8.3:6662694-6662713 | MS.gene061113:CDS | 70.0% |
| !! | GGCTTTGGAGGCGGTGCTGG+AGG | + | chr8.3:6662757-6662776 | MS.gene061113:CDS | 70.0% |
| !! | GGTGGCTTTGGAGGCGGTGC+TGG | + | chr8.3:6662754-6662773 | MS.gene061113:CDS | 70.0% |
| !!! | GGTGCCGGCGGAGGTTTTGG+TGG | + | chr8.3:6662691-6662710 | MS.gene061113:CDS | 70.0% |
| !!! | GGTGGTGCCGGCGGAGGTTT+TGG | + | chr8.3:6662688-6662707 | MS.gene061113:CDS | 70.0% |
| GGTGGAGGTGGCGTGGGTGG+TGG | + | chr8.3:6662670-6662689 | MS.gene061113:CDS | 75.0% | |
| ! | GGCGGTGCTGGAGGTGGTGC+AGG | + | chr8.3:6662766-6662785 | MS.gene061113:CDS | 75.0% |
| ! | GGTGGCGTGGGTGGTGGTGC+CGG | + | chr8.3:6662676-6662695 | MS.gene061113:CDS | 75.0% |
| !! | GGCGTGGGTGGTGGTGCCGG+CGG | + | chr8.3:6662679-6662698 | MS.gene061113:CDS | 80.0% |
| !!! | GTGGGTGGTGGTGCCGGCGG+AGG | + | chr8.3:6662682-6662701 | MS.gene061113:CDS | 80.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 6662562 | 6662936 | 6662562 | ID=MS.gene061113 |
| chr8.3 | mRNA | 6662562 | 6662936 | 6662562 | ID=MS.gene061113.t1;Parent=MS.gene061113 |
| chr8.3 | exon | 6662562 | 6662936 | 6662562 | ID=MS.gene061113.t1.exon1;Parent=MS.gene061113.t1 |
| chr8.3 | CDS | 6662562 | 6662936 | 6662562 | ID=cds.MS.gene061113.t1;Parent=MS.gene061113.t1 |
| Gene Sequence |
| Protein sequence |
