Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45558.t1 | RHN79491.1 | 99.5 | 183 | 1 | 0 | 1 | 183 | 1 | 183 | 4.70E-101 | 377.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45558.t1 | Q6GM21 | 31.9 | 182 | 110 | 6 | 2 | 179 | 1 | 172 | 1.4e-16 | 87.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45558.t1 | A0A396JRU3 | 99.5 | 183 | 1 | 0 | 1 | 183 | 1 | 183 | 3.4e-101 | 377.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049807 | MS.gene45558 | 0.804569 | 2.03E-49 | -1.69E-46 |
| MS.gene050945 | MS.gene45558 | 0.800587 | 1.34E-48 | -1.69E-46 |
| MS.gene051676 | MS.gene45558 | 0.810672 | 1.03E-50 | -1.69E-46 |
| MS.gene051699 | MS.gene45558 | 0.819102 | 1.39E-52 | -1.69E-46 |
| MS.gene051902 | MS.gene45558 | 0.823914 | 1.07E-53 | -1.69E-46 |
| MS.gene052239 | MS.gene45558 | -0.80197 | 7.00E-49 | -1.69E-46 |
| MS.gene052242 | MS.gene45558 | -0.807154 | 5.81E-50 | -1.69E-46 |
| MS.gene052533 | MS.gene45558 | 0.807023 | 6.19E-50 | -1.69E-46 |
| MS.gene052932 | MS.gene45558 | 0.801018 | 1.10E-48 | -1.69E-46 |
| MS.gene053298 | MS.gene45558 | 0.805291 | 1.43E-49 | -1.69E-46 |
| MS.gene054222 | MS.gene45558 | 0.838755 | 2.38E-57 | -1.69E-46 |
| MS.gene056677 | MS.gene45558 | 0.803877 | 2.83E-49 | -1.69E-46 |
| MS.gene057102 | MS.gene45558 | 0.810968 | 8.85E-51 | -1.69E-46 |
| MS.gene05985 | MS.gene45558 | -0.8275 | 1.51E-54 | -1.69E-46 |
| MS.gene059965 | MS.gene45558 | 0.827448 | 1.56E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45558.t1 | MTR_8g096810 | 98.361 | 183 | 3 | 0 | 1 | 183 | 1 | 183 | 5.27e-136 | 377 |
| MS.gene45558.t1 | MTR_1g058050 | 98.907 | 183 | 2 | 0 | 1 | 183 | 77 | 259 | 1.24e-135 | 379 |
| MS.gene45558.t1 | MTR_2g098170 | 98.361 | 183 | 3 | 0 | 1 | 183 | 1 | 183 | 2.83e-135 | 375 |
| MS.gene45558.t1 | MTR_2g017985 | 97.268 | 183 | 5 | 0 | 1 | 183 | 1 | 183 | 8.94e-135 | 374 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45558.t1 | AT5G13070 | 72.376 | 181 | 48 | 1 | 1 | 179 | 1 | 181 | 4.84e-102 | 291 |
Find 41 sgRNAs with CRISPR-Local
Find 43 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTGTCTGCTTTGGCTTCTA+TGG | 0.285179 | 2.1:+6130298 | MS.gene45558:CDS |
| GGTCGATGCAGATTTGTTAT+AGG | 0.304123 | 2.1:+6130159 | MS.gene45558:CDS |
| TGCTAAGAGTAGAGATGTTA+TGG | 0.314934 | 2.1:+6130367 | MS.gene45558:CDS |
| GTCCATCCATTCGGATTATC+AGG | 0.325224 | 2.1:-6130239 | None:intergenic |
| TTGTTATAGGAATATTAGTA+TGG | 0.335683 | 2.1:+6130172 | MS.gene45558:CDS |
| GATTAAACCATTGTCTGCTT+TGG | 0.373627 | 2.1:+6130289 | MS.gene45558:CDS |
| ACAAACCTTGATTCATCCTC+TGG | 0.420030 | 2.1:+6130023 | MS.gene45558:CDS |
| AACCGTTCGTTGTCCGTGGC+TGG | 0.420484 | 2.1:+6130070 | MS.gene45558:CDS |
| TGGCTGGTACGAAGGATTAT+CGG | 0.421617 | 2.1:+6130086 | MS.gene45558:CDS |
| GTCAGCAGGAGACGAGGATT+CGG | 0.428182 | 2.1:+6130267 | MS.gene45558:CDS |
| TTGAGCAGAGGTGTGCTGAT+AGG | 0.440113 | 2.1:+6130330 | MS.gene45558:CDS |
| TATGGAGAAGTTTATTGAAG+TGG | 0.479069 | 2.1:+6130190 | MS.gene45558:CDS |
| GTTGTCCGTGGCTGGTACGA+AGG | 0.479907 | 2.1:+6130078 | MS.gene45558:CDS |
| AACATGTTTATAACCATCCA+TGG | 0.481341 | 2.1:+6129910 | MS.gene45558:CDS |
| AGGTTACTCGTTCCCATGGA+TGG | 0.512718 | 2.1:-6129923 | None:intergenic |
| TGGATGGACTGTTTGTCAGC+AGG | 0.536254 | 2.1:+6130253 | MS.gene45558:CDS |
| GCCGAGGTTACTCGTTCCCA+TGG | 0.538501 | 2.1:-6129927 | None:intergenic |
| GTCTGCTTTGGCTTCTATGG+CGG | 0.542260 | 2.1:+6130301 | MS.gene45558:CDS |
| TGACAAACAGTCCATCCATT+CGG | 0.545003 | 2.1:-6130248 | None:intergenic |
| TTGAAGTGGAAGAGAAGACT+AGG | 0.557364 | 2.1:+6130204 | MS.gene45558:CDS |
| AACGAGTAACCTCGGCATCA+TGG | 0.564625 | 2.1:+6129934 | MS.gene45558:CDS |
| GCTTCCCAGAGGATGAATCA+AGG | 0.569741 | 2.1:-6130028 | None:intergenic |
| ATCCTGATAATCCGAATGGA+TGG | 0.575753 | 2.1:+6130237 | MS.gene45558:CDS |
| TACCAGCCACGGACAACGAA+CGG | 0.582782 | 2.1:-6130072 | None:intergenic |
| CTATAACCGTTCGTTGTCCG+TGG | 0.586902 | 2.1:+6130066 | MS.gene45558:CDS |
| CTGTTTGTCAGCAGGAGACG+AGG | 0.587525 | 2.1:+6130261 | MS.gene45558:CDS |
| GAGGATTTGCAAATATCTTG+AGG | 0.598037 | 2.1:+6130391 | MS.gene45558:CDS |
| CCTCATCCTGATAATCCGAA+TGG | 0.599257 | 2.1:+6130233 | MS.gene45558:CDS |
| CCATTCGGATTATCAGGATG+AGG | 0.600517 | 2.1:-6130233 | None:intergenic |
| AGAGTAGAGATGTTATGGAG+AGG | 0.605987 | 2.1:+6130372 | MS.gene45558:CDS |
| CAAACCTTGATTCATCCTCT+GGG | 0.615316 | 2.1:+6130024 | MS.gene45558:CDS |
| TCCATGGGAACGAGTAACCT+CGG | 0.631582 | 2.1:+6129926 | MS.gene45558:CDS |
| ATAATCCTTCGTACCAGCCA+CGG | 0.637876 | 2.1:-6130083 | None:intergenic |
| ATAGAAGCCAAAGCAGACAA+TGG | 0.655161 | 2.1:-6130296 | None:intergenic |
| ACATGTTTATAACCATCCAT+GGG | 0.656615 | 2.1:+6129911 | MS.gene45558:CDS |
| TGGCGGAGAAAGTTGAGCAG+AGG | 0.668340 | 2.1:+6130318 | MS.gene45558:CDS |
| TTGTGTTGAATCAACTGTCG+TGG | 0.677952 | 2.1:+6130124 | MS.gene45558:CDS |
| TTATATGAGAAACAATCACA+AGG | 0.723613 | 2.1:-6130432 | None:intergenic |
| CTGTCGTGGATGCAAAATCG+CGG | 0.724194 | 2.1:+6130138 | MS.gene45558:CDS |
| GTATTGCAATCGAGAATGTG+TGG | 0.729957 | 2.1:-6129993 | None:intergenic |
| TGTCGCGTATAGCTTCCCAG+AGG | 0.828110 | 2.1:-6130039 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTGTTATAGGAATATTAGTA+TGG | + | chr2.1:6130172-6130191 | MS.gene45558:CDS | 20.0% |
| AACATGTTTATAACCATCCA+TGG | + | chr2.1:6129910-6129929 | MS.gene45558:CDS | 30.0% | |
| ACATGTTTATAACCATCCAT+GGG | + | chr2.1:6129911-6129930 | MS.gene45558:CDS | 30.0% | |
| TATGGAGAAGTTTATTGAAG+TGG | + | chr2.1:6130190-6130209 | MS.gene45558:CDS | 30.0% | |
| !! | TGGTAAAACACGTTTGTTTT+CGG | - | chr2.1:6129976-6129995 | None:intergenic | 30.0% |
| GAGGATTTGCAAATATCTTG+AGG | + | chr2.1:6130391-6130410 | MS.gene45558:CDS | 35.0% | |
| GATTAAACCATTGTCTGCTT+TGG | + | chr2.1:6130289-6130308 | MS.gene45558:CDS | 35.0% | |
| TGCTAAGAGTAGAGATGTTA+TGG | + | chr2.1:6130367-6130386 | MS.gene45558:CDS | 35.0% | |
| ACAAACCTTGATTCATCCTC+TGG | + | chr2.1:6130023-6130042 | MS.gene45558:CDS | 40.0% | |
| AGAGTAGAGATGTTATGGAG+AGG | + | chr2.1:6130372-6130391 | MS.gene45558:CDS | 40.0% | |
| ATAGAAGCCAAAGCAGACAA+TGG | - | chr2.1:6130299-6130318 | None:intergenic | 40.0% | |
| ATCCTGATAATCCGAATGGA+TGG | + | chr2.1:6130237-6130256 | MS.gene45558:CDS | 40.0% | |
| CAAACCTTGATTCATCCTCT+GGG | + | chr2.1:6130024-6130043 | MS.gene45558:CDS | 40.0% | |
| GTATTGCAATCGAGAATGTG+TGG | - | chr2.1:6129996-6130015 | None:intergenic | 40.0% | |
| TGACAAACAGTCCATCCATT+CGG | - | chr2.1:6130251-6130270 | None:intergenic | 40.0% | |
| TTGAAGTGGAAGAGAAGACT+AGG | + | chr2.1:6130204-6130223 | MS.gene45558:CDS | 40.0% | |
| TTGTGTTGAATCAACTGTCG+TGG | + | chr2.1:6130124-6130143 | MS.gene45558:CDS | 40.0% | |
| ! | ATTGTCTGCTTTGGCTTCTA+TGG | + | chr2.1:6130298-6130317 | MS.gene45558:CDS | 40.0% |
| !! | GGTCGATGCAGATTTGTTAT+AGG | + | chr2.1:6130159-6130178 | MS.gene45558:CDS | 40.0% |
| ATAATCCTTCGTACCAGCCA+CGG | - | chr2.1:6130086-6130105 | None:intergenic | 45.0% | |
| CCATTCGGATTATCAGGATG+AGG | - | chr2.1:6130236-6130255 | None:intergenic | 45.0% | |
| CCTCATCCTGATAATCCGAA+TGG | + | chr2.1:6130233-6130252 | MS.gene45558:CDS | 45.0% | |
| GTCCATCCATTCGGATTATC+AGG | - | chr2.1:6130242-6130261 | None:intergenic | 45.0% | |
| TGGCTGGTACGAAGGATTAT+CGG | + | chr2.1:6130086-6130105 | MS.gene45558:CDS | 45.0% | |
| !! | AACACGTTTGTTTTCGGCGT+CGG | - | chr2.1:6129970-6129989 | None:intergenic | 45.0% |
| AACGAGTAACCTCGGCATCA+TGG | + | chr2.1:6129934-6129953 | MS.gene45558:CDS | 50.0% | |
| AGGTTACTCGTTCCCATGGA+TGG | - | chr2.1:6129926-6129945 | None:intergenic | 50.0% | |
| CTATAACCGTTCGTTGTCCG+TGG | + | chr2.1:6130066-6130085 | MS.gene45558:CDS | 50.0% | |
| CTGTCGTGGATGCAAAATCG+CGG | + | chr2.1:6130138-6130157 | MS.gene45558:CDS | 50.0% | |
| GCTTCCCAGAGGATGAATCA+AGG | - | chr2.1:6130031-6130050 | None:intergenic | 50.0% | |
| TCCATGGGAACGAGTAACCT+CGG | + | chr2.1:6129926-6129945 | MS.gene45558:CDS | 50.0% | |
| ! | GAATTTTCGCCATGATGCCG+AGG | - | chr2.1:6129946-6129965 | None:intergenic | 50.0% |
| ! | GTCTGCTTTGGCTTCTATGG+CGG | + | chr2.1:6130301-6130320 | MS.gene45558:CDS | 50.0% |
| ! | TTGAGCAGAGGTGTGCTGAT+AGG | + | chr2.1:6130330-6130349 | MS.gene45558:CDS | 50.0% |
| !! | TGGATGGACTGTTTGTCAGC+AGG | + | chr2.1:6130253-6130272 | MS.gene45558:CDS | 50.0% |
| CTGTTTGTCAGCAGGAGACG+AGG | + | chr2.1:6130261-6130280 | MS.gene45558:CDS | 55.0% | |
| GTCAGCAGGAGACGAGGATT+CGG | + | chr2.1:6130267-6130286 | MS.gene45558:CDS | 55.0% | |
| TACCAGCCACGGACAACGAA+CGG | - | chr2.1:6130075-6130094 | None:intergenic | 55.0% | |
| TGGCGGAGAAAGTTGAGCAG+AGG | + | chr2.1:6130318-6130337 | MS.gene45558:CDS | 55.0% | |
| TGTCGCGTATAGCTTCCCAG+AGG | - | chr2.1:6130042-6130061 | None:intergenic | 55.0% | |
| AACCGTTCGTTGTCCGTGGC+TGG | + | chr2.1:6130070-6130089 | MS.gene45558:CDS | 60.0% | |
| GCCGAGGTTACTCGTTCCCA+TGG | - | chr2.1:6129930-6129949 | None:intergenic | 60.0% | |
| GTTGTCCGTGGCTGGTACGA+AGG | + | chr2.1:6130078-6130097 | MS.gene45558:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.1 | gene | 6129888 | 6130439 | 6129888 | ID=MS.gene45558 |
| chr2.1 | mRNA | 6129888 | 6130439 | 6129888 | ID=MS.gene45558.t1;Parent=MS.gene45558 |
| chr2.1 | exon | 6129888 | 6130439 | 6129888 | ID=MS.gene45558.t1.exon1;Parent=MS.gene45558.t1 |
| chr2.1 | CDS | 6129888 | 6130439 | 6129888 | ID=cds.MS.gene45558.t1;Parent=MS.gene45558.t1 |
| Gene Sequence |
| Protein sequence |
