AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene72499

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene72499.t1 MTR_4g035980 97.222 540 14 1 1 540 1 539 0.0 1098
MS.gene72499.t1 MTR_3g008640 66.234 539 166 7 4 540 3 527 0.0 721
MS.gene72499.t1 MTR_3g008720 65.677 539 169 7 4 540 3 527 0.0 717
MS.gene72499.t1 MTR_3g033690 64.815 540 171 8 4 540 3 526 0.0 712
MS.gene72499.t1 MTR_7g053360 64.750 539 174 7 4 540 3 527 0.0 710
MS.gene72499.t1 MTR_3g008700 65.121 539 172 7 4 540 3 527 0.0 710
MS.gene72499.t1 MTR_4g025690 51.172 512 231 9 29 540 21 513 1.11e-175 506
MS.gene72499.t1 MTR_4g025730 48.127 534 246 12 8 540 6 509 1.47e-167 484
MS.gene72499.t1 MTR_8g104655 45.681 521 248 10 40 540 36 541 5.67e-161 469
MS.gene72499.t1 MTR_7g108905 48.989 445 205 8 107 536 115 552 2.59e-137 409
MS.gene72499.t1 MTR_8g023310 43.561 528 261 11 40 540 72 589 5.73e-134 402
MS.gene72499.t1 MTR_8g104645 54.094 342 152 2 203 540 32 372 8.06e-134 394
MS.gene72499.t1 MTR_7g050980 47.248 436 222 5 108 540 125 555 5.24e-132 395
MS.gene72499.t1 MTR_8g104580 41.075 521 239 11 40 540 28 500 5.29e-132 394
MS.gene72499.t1 MTR_3g091380 40.972 576 291 14 3 540 33 597 7.77e-132 397
MS.gene72499.t1 MTR_4g025670 47.758 446 198 11 107 540 67 489 2.30e-131 392
MS.gene72499.t1 MTR_1g008140 40.172 580 295 13 3 540 38 607 3.37e-130 393
MS.gene72499.t1 MTR_7g050870 46.101 436 227 5 108 540 124 554 9.51e-128 384
MS.gene72499.t1 MTR_4g087980 45.824 443 215 8 108 540 89 516 4.61e-125 376
MS.gene72499.t1 MTR_2g044880 44.079 456 239 7 92 540 79 525 7.17e-123 371
MS.gene72499.t1 MTR_7g050950 43.678 435 236 6 109 540 125 553 1.90e-121 368
MS.gene72499.t1 MTR_2g044810 38.745 542 311 11 3 540 10 534 1.06e-119 363
MS.gene72499.t1 MTR_1g088020 41.002 539 265 13 40 540 32 555 1.68e-119 363
MS.gene72499.t1 MTR_7g112580 43.764 457 230 9 98 540 99 542 1.88e-118 360
MS.gene72499.t1 MTR_1g054880 53.776 331 146 4 215 540 230 558 3.91e-117 357
MS.gene72499.t1 MTR_7g108900 41.909 482 238 10 91 536 81 556 6.75e-117 357
MS.gene72499.t1 MTR_4g087970 40.881 477 253 8 78 540 56 517 8.47e-116 353
MS.gene72499.t1 MTR_8g042910 43.070 469 214 12 107 540 162 612 4.01e-115 354
MS.gene72499.t1 MTR_1g083520 39.640 555 288 15 2 540 5 528 3.82e-114 348
MS.gene72499.t1 MTR_7g108890 40.038 532 279 15 40 540 40 562 1.14e-113 348
MS.gene72499.t1 MTR_7g108875 38.649 533 285 12 40 540 42 564 1.54e-113 348
MS.gene72499.t1 MTR_1g088225 40.260 539 267 14 40 540 32 553 2.89e-113 347
MS.gene72499.t1 MTR_4g124520 38.113 530 295 11 16 540 20 521 1.27e-112 345
MS.gene72499.t1 MTR_3g010780 42.825 446 230 10 107 540 142 574 1.48e-112 346
MS.gene72499.t1 MTR_0458s0030 58.484 277 111 3 266 540 40 314 4.52e-110 331
MS.gene72499.t1 MTR_1g088040 58.484 277 111 3 266 540 40 314 4.52e-110 331
MS.gene72499.t1 MTR_3g010770 50.595 336 158 4 211 540 242 575 7.05e-110 339
MS.gene72499.t1 MTR_8g042900 43.439 442 231 10 108 540 150 581 4.83e-109 337
MS.gene72499.t1 MTR_4g124550 36.170 517 306 10 40 540 85 593 5.89e-104 325
MS.gene72499.t1 MTR_8g023150 37.403 516 294 14 40 540 81 582 2.52e-101 317
MS.gene72499.t1 MTR_0618s0020 60.526 228 89 1 313 540 2 228 1.31e-100 303
MS.gene72499.t1 MTR_1g083470 34.259 540 317 14 20 536 42 566 1.50e-95 302
MS.gene72499.t1 MTR_1g083500 34.259 540 317 14 20 536 42 566 1.50e-95 302
MS.gene72499.t1 MTR_1g086210 43.785 354 190 5 194 540 218 569 3.66e-95 301
MS.gene72499.t1 MTR_1g083510 37.209 473 241 12 109 539 124 582 5.29e-95 301
MS.gene72499.t1 MTR_1g086190 35.785 503 290 11 63 540 69 563 3.54e-93 296
MS.gene72499.t1 MTR_7g112590 33.821 547 322 16 23 539 46 582 1.09e-92 295
MS.gene72499.t1 MTR_1g083490 38.479 447 237 11 106 536 147 571 1.41e-92 295
MS.gene72499.t1 MTR_2g436050 33.393 560 321 16 12 539 24 563 3.51e-92 293
MS.gene72499.t1 MTR_4g005170 38.031 447 239 11 106 536 147 571 3.89e-91 292
MS.gene72499.t1 MTR_4g120290 32.453 530 323 15 32 539 55 571 6.49e-91 290
MS.gene72499.t1 MTR_4g005180 37.136 447 243 10 106 536 129 553 1.96e-90 288
MS.gene72499.t1 MTR_4g120280 32.821 521 317 11 40 539 63 571 8.86e-90 287
MS.gene72499.t1 MTR_2g436040 32.407 540 341 14 10 537 16 543 1.88e-89 287
MS.gene72499.t1 MTR_2g044810 52.157 255 120 2 286 540 213 465 7.92e-88 278
MS.gene72499.t1 MTR_8g093460 34.774 532 307 14 23 532 30 543 1.75e-84 273
MS.gene72499.t1 MTR_2g044660 44.904 314 166 6 227 540 326 632 2.64e-84 274
MS.gene72499.t1 MTR_8g088610 40.841 333 192 3 206 537 45 373 4.57e-84 266
MS.gene72499.t1 MTR_5g083620 39.193 347 199 5 200 540 16 356 7.65e-84 265
MS.gene72499.t1 MTR_3g095920 34.722 576 312 18 2 539 14 563 1.04e-83 271
MS.gene72499.t1 MTR_7g110490 42.486 346 186 4 201 535 226 569 2.46e-82 268
MS.gene72499.t1 MTR_7g110510 42.486 346 186 4 201 535 226 569 2.46e-82 268
MS.gene72499.t1 MTR_7g110560 40.988 344 196 3 201 540 209 549 4.09e-81 264
MS.gene72499.t1 MTR_7g110440 37.413 433 240 8 137 540 144 574 1.95e-80 263
MS.gene72499.t1 MTR_2g105560 39.683 315 174 5 227 540 40 339 2.12e-73 237
MS.gene72499.t1 MTR_7g107180 39.241 316 178 5 226 540 5 307 5.53e-73 235
MS.gene72499.t1 MTR_7g110420 38.418 354 203 5 201 540 226 578 8.83e-70 234
MS.gene72499.t1 MTR_7g111560 30.521 557 338 17 3 539 28 555 1.38e-63 218
MS.gene72499.t1 MTR_0458s0010 31.054 541 279 17 40 540 32 518 2.27e-59 205
MS.gene72499.t1 MTR_7g110430 35.043 351 181 5 201 540 226 540 5.32e-59 205
MS.gene72499.t1 MTR_6g012800 36.491 285 158 5 228 502 6 277 5.75e-55 188
MS.gene72499.t1 MTR_6g088810 36.721 305 165 8 236 529 90 377 3.09e-52 182
MS.gene72499.t1 MTR_7g011530 38.112 286 148 7 230 502 8 277 5.08e-52 180
MS.gene72499.t1 MTR_1g086540 35.714 322 180 7 218 529 40 344 1.20e-51 180
MS.gene72499.t1 MTR_6g024165 34.713 314 173 8 228 529 41 334 3.07e-51 179
MS.gene72499.t1 MTR_2g039530 35.897 312 182 8 199 507 23 319 7.07e-51 177
MS.gene72499.t1 MTR_8g102380 33.962 371 198 14 173 529 40 377 1.15e-50 178
MS.gene72499.t1 MTR_7g092780 34.062 320 187 8 220 529 82 387 1.54e-45 165
MS.gene72499.t1 MTR_5g083590 30.408 319 169 5 223 540 2 268 8.09e-44 157
MS.gene72499.t1 MTR_7g102600 34.416 308 179 8 230 529 101 393 1.31e-43 160
MS.gene72499.t1 MTR_4g124740 32.641 337 180 12 208 529 35 339 2.44e-42 155
MS.gene72499.t1 MTR_5g093970 31.908 304 188 6 228 529 43 329 3.10e-42 154
MS.gene72499.t1 MTR_5g095490 35.275 309 169 9 234 529 67 357 3.25e-42 155
MS.gene72499.t1 MTR_8g102380 33.962 318 167 12 173 476 40 328 1.58e-41 152
MS.gene72499.t1 MTR_5g012600 32.278 316 187 8 228 532 63 362 1.59e-40 150
MS.gene72499.t1 MTR_6g042660 34.643 280 158 8 228 502 37 296 9.25e-40 147
MS.gene72499.t1 MTR_5g088300 33.443 305 175 11 228 529 37 316 2.07e-38 144
MS.gene72499.t1 MTR_5g088280 31.613 310 182 10 224 529 35 318 1.61e-36 138
MS.gene72499.t1 MTR_6g042690 34.843 287 153 11 228 502 39 303 4.97e-36 137
MS.gene72499.t1 MTR_4g051810 33.096 281 167 7 223 502 32 292 5.67e-36 137
MS.gene72499.t1 MTR_1g033050 33.916 286 158 10 230 502 17 284 1.06e-35 136
MS.gene72499.t1 MTR_2g033970 31.250 320 187 9 221 531 53 348 3.47e-35 135
MS.gene72499.t1 MTR_5g088290 33.935 277 161 7 228 502 48 304 8.28e-35 134
MS.gene72499.t1 MTR_4g074290 29.674 337 204 13 209 529 42 361 1.10e-33 131
MS.gene72499.t1 MTR_2g073390 29.464 336 206 10 209 529 42 361 3.09e-33 130
MS.gene72499.t1 MTR_8g036225 32.168 286 163 8 228 502 33 298 3.04e-32 127
MS.gene72499.t1 MTR_0009s0350 30.000 340 199 14 209 529 42 361 1.57e-31 125
MS.gene72499.t1 MTR_0009s0340 30.000 340 199 14 209 529 42 361 1.57e-31 125
MS.gene72499.t1 MTR_2g436600 30.154 325 175 12 221 531 52 338 3.16e-29 118
MS.gene72499.t1 MTR_6g025590 35.196 179 107 3 230 400 51 228 1.03e-26 108
MS.gene72499.t1 MTR_2g436070 25.962 312 194 13 12 300 24 321 7.97e-18 85.9
MS.gene72499.t1 MTR_4g130790 32.727 165 97 5 257 412 53 212 6.06e-17 81.3
MS.gene72499.t1 MTR_0408s0030 39.796 98 49 2 405 502 36 123 1.74e-15 74.3
MS.gene72499.t1 MTR_6g024200 32.768 177 90 6 357 529 125 276 2.59e-14 73.9
MS.gene72499.t1 MTR_7g102570 30.645 124 73 3 388 509 3 115 8.05e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene72499.t1 AT3G47400 57.358 530 212 7 21 540 69 594 0.0 612
MS.gene72499.t1 AT5G51490 57.480 508 202 7 39 540 37 536 0.0 605
MS.gene72499.t1 AT5G51500 58.171 514 203 8 32 540 34 540 0.0 600
MS.gene72499.t1 AT2G45220 48.885 538 240 14 4 540 8 511 8.80e-170 490
MS.gene72499.t1 AT1G23200 43.258 534 253 9 40 540 38 554 3.98e-144 427
MS.gene72499.t1 AT4G00190 42.885 506 245 12 40 540 8 474 4.45e-133 395
MS.gene72499.t1 AT3G60730 45.356 463 209 10 108 540 71 519 4.26e-131 392
MS.gene72499.t1 AT1G11580 48.411 409 199 6 137 540 156 557 1.88e-126 381
MS.gene72499.t1 AT1G11580 48.642 405 196 6 137 536 177 574 6.54e-126 380
MS.gene72499.t1 AT5G53370 44.536 485 246 10 74 540 106 585 4.17e-125 379
MS.gene72499.t1 AT1G53830 42.351 536 260 13 40 540 66 587 5.28e-125 379
MS.gene72499.t1 AT5G53370 44.536 485 246 10 74 540 142 621 1.59e-124 379
MS.gene72499.t1 AT3G49220 40.502 558 294 13 8 540 52 596 3.75e-124 377
MS.gene72499.t1 AT3G14310 44.654 477 227 9 98 540 119 592 1.39e-123 375
MS.gene72499.t1 AT3G49220 40.502 558 294 13 8 540 97 641 2.12e-123 376
MS.gene72499.t1 AT4G02320 45.824 443 226 8 108 540 80 518 1.43e-122 370
MS.gene72499.t1 AT3G59010 44.444 477 236 11 75 540 70 528 8.21e-122 369
MS.gene72499.t1 AT3G43270 44.743 447 229 7 108 540 83 525 1.16e-121 368
MS.gene72499.t1 AT4G02330 38.889 576 296 12 9 540 8 571 2.97e-120 366
MS.gene72499.t1 AT4G33220 42.443 483 250 8 83 540 44 523 4.46e-118 358
MS.gene72499.t1 AT4G02300 42.070 454 245 8 98 540 86 532 2.38e-117 357
MS.gene72499.t1 AT4G33220 51.873 347 164 3 194 540 59 402 7.30e-117 351
MS.gene72499.t1 AT3G05620 43.064 483 231 13 77 540 84 541 2.85e-116 355
MS.gene72499.t1 AT5G04960 38.616 549 299 14 8 538 34 562 5.82e-116 355
MS.gene72499.t1 AT2G43050 42.918 473 241 10 75 540 66 516 9.18e-116 352
MS.gene72499.t1 AT1G02810 38.540 589 288 17 8 540 7 577 1.11e-111 344
MS.gene72499.t1 AT3G05610 34.464 560 337 11 1 540 16 565 1.58e-110 344
MS.gene72499.t1 AT5G27870 37.476 515 295 11 40 540 61 562 1.95e-110 345
MS.gene72499.t1 AT5G20860 35.624 553 323 12 4 540 3 538 2.01e-110 339
MS.gene72499.t1 AT3G10710 40.146 548 293 14 3 540 39 561 2.24e-110 340
MS.gene72499.t1 AT2G47550 38.256 562 306 11 9 540 8 558 1.21e-107 333
MS.gene72499.t1 AT5G49180 35.563 568 318 13 3 536 13 566 3.37e-107 332
MS.gene72499.t1 AT3G10720 38.376 542 284 13 40 540 83 615 2.15e-106 332
MS.gene72499.t1 AT2G26440 38.269 520 291 9 39 540 40 547 2.24e-106 329
MS.gene72499.t1 AT2G26450 40.931 408 231 7 137 540 210 611 1.49e-103 324
MS.gene72499.t1 AT4G33230 35.755 523 307 10 36 540 95 606 3.84e-103 323
MS.gene72499.t1 AT1G53840 36.685 537 302 12 22 534 59 581 8.62e-102 318
MS.gene72499.t1 AT3G27980 43.672 403 215 8 139 540 106 497 2.64e-101 315
MS.gene72499.t1 AT5G04970 37.153 541 292 14 40 540 88 620 3.66e-101 318
MS.gene72499.t1 AT3G06830 39.524 420 232 10 137 538 151 566 6.51e-101 316
MS.gene72499.t1 AT1G11590 43.489 407 218 7 137 540 127 524 1.68e-100 313
MS.gene72499.t1 AT4G03930 42.963 405 219 8 139 540 129 524 1.16e-99 311
MS.gene72499.t1 AT3G10720 55.939 261 113 2 280 540 1 259 1.02e-98 300
MS.gene72499.t1 AT3G14300 36.852 521 287 14 40 535 463 966 1.74e-92 303
MS.gene72499.t1 AT5G20860 32.563 519 290 10 4 506 3 477 3.96e-87 278
MS.gene72499.t1 AT4G15980 45.797 345 175 8 201 538 361 700 6.54e-87 283
MS.gene72499.t1 AT4G15980 45.797 345 175 8 201 538 371 710 7.61e-87 283
MS.gene72499.t1 AT5G09760 35.261 536 296 16 23 528 32 546 2.67e-83 270
MS.gene72499.t1 AT5G09760 35.261 536 296 16 23 528 58 572 4.25e-83 270
MS.gene72499.t1 AT2G47040 41.341 358 191 8 196 540 244 595 4.97e-80 262
MS.gene72499.t1 AT1G11370 48.988 247 125 1 225 471 43 288 1.13e-79 251
MS.gene72499.t1 AT2G47030 40.390 359 196 6 196 540 234 588 4.80e-79 259
MS.gene72499.t1 AT5G64640 39.243 423 222 13 137 533 189 602 4.57e-77 254
MS.gene72499.t1 AT3G62170 37.602 367 203 6 196 540 226 588 3.09e-71 239
MS.gene72499.t1 AT2G36700 35.127 316 183 7 220 529 34 333 7.39e-52 180
MS.gene72499.t1 AT3G29090 36.749 283 156 6 230 502 9 278 2.61e-51 178
MS.gene72499.t1 AT5G19730 33.535 331 188 9 210 529 72 381 1.42e-50 178
MS.gene72499.t1 AT2G36710 32.602 319 192 8 225 534 86 390 8.42e-48 171
MS.gene72499.t1 AT2G21610 34.314 306 178 8 228 531 51 335 1.97e-46 166
MS.gene72499.t1 AT2G21610 34.314 306 178 8 228 531 56 340 2.56e-46 166
MS.gene72499.t1 AT1G05310 34.069 317 179 8 226 529 89 388 7.34e-46 166
MS.gene72499.t1 AT5G47500 31.515 330 193 9 213 529 48 357 1.11e-40 151
MS.gene72499.t1 AT5G07430 31.751 337 199 10 207 529 36 355 3.58e-40 149
MS.gene72499.t1 AT3G17060 32.797 311 182 9 228 530 46 337 6.06e-40 148
MS.gene72499.t1 AT3G24130 33.667 300 163 9 219 502 25 304 6.56e-38 142
MS.gene72499.t1 AT1G44980 42.105 190 106 3 207 393 54 242 4.07e-37 138
MS.gene72499.t1 AT5G55590 32.133 361 211 12 183 529 38 378 7.98e-37 140
MS.gene72499.t1 AT5G55590 33.553 304 172 10 183 472 38 325 1.51e-35 136
MS.gene72499.t1 AT5G61680 31.111 315 187 10 222 529 67 358 2.04e-34 134
MS.gene72499.t1 AT5G07410 30.247 324 194 11 197 502 22 331 2.92e-33 130
MS.gene72499.t1 AT5G26810 31.973 294 162 9 228 502 34 308 4.64e-33 129
MS.gene72499.t1 AT1G69940 30.247 324 194 11 197 502 22 331 5.23e-33 129
MS.gene72499.t1 AT5G18990 32.069 290 165 8 227 502 28 299 5.63e-33 129
MS.gene72499.t1 AT5G07420 29.499 339 206 11 206 529 35 355 7.74e-32 126
MS.gene72499.t1 AT2G19150 29.483 329 196 9 221 536 34 339 6.46e-31 123
MS.gene72499.t1 AT2G19150 30.943 265 151 7 221 472 34 279 1.51e-28 115
MS.gene72499.t1 AT3G49220 28.049 328 199 12 8 310 97 412 3.28e-24 105
MS.gene72499.t1 AT2G47280 30.272 294 169 10 224 502 31 303 2.97e-23 101

Find 132 sgRNAs with CRISPR-Local

Find 162 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGTAGTATTTGCATGTTTC+TGG 0.063526 4.1:-64375123 None:intergenic
ATAATTGGTACAATGAATTT+TGG 0.141708 4.1:-64375471 None:intergenic
ATGTTTATAACTTTCATAAT+TGG 0.257834 4.1:-64375486 None:intergenic
CCGACAACGGGCTTGAAATC+TGG 0.267143 4.1:-64376459 None:intergenic
ATGTTTGCTTGGCCGTTTAA+AGG 0.275130 4.1:-64376360 None:intergenic
GGAACCTTCAAGGGTTATTA+AGG 0.287555 4.1:+64375641 MS.gene72499:CDS
GTACAAGAATGATGCAGTTT+TGG 0.293323 4.1:-64376332 None:intergenic
TACAGAGAATGTTACATCTA+TGG 0.298252 4.1:+64376270 MS.gene72499:CDS
GAAATCATTCATGGATAGTT+TGG 0.300212 4.1:+64376539 MS.gene72499:CDS
GGCACCGTTGACTTCATCTT+TGG 0.305498 4.1:+64376291 MS.gene72499:CDS
GCACAAAGCACTCTATTCTA+TGG 0.307657 4.1:+64376603 MS.gene72499:CDS
CAATTCACAACTCTCAGATT+AGG 0.313122 4.1:+64376430 MS.gene72499:CDS
CATCTTGTACTCTCTTAAAA+TGG 0.314188 4.1:-64375695 None:intergenic
AATTCACAACTCTCAGATTA+GGG 0.320861 4.1:+64376431 MS.gene72499:CDS
GCAATGGTTTATTCGTCAAT+TGG 0.333480 4.1:+64375523 MS.gene72499:CDS
TTGTGTACTATAGGAATTGA+TGG 0.340716 4.1:+64376087 MS.gene72499:intron
ACCGTTGCTACCCTCCTTGC+TGG 0.365327 4.1:+64376723 MS.gene72499:CDS
TTGCCAGGGCCGGCCCAAAA+AGG 0.367122 4.1:-64376493 None:intergenic
CTATTTCTTGTTGATGATCC+AGG 0.370812 4.1:-64376645 None:intergenic
GTCCAATGGGGTGGTCTCCA+TGG 0.375821 4.1:+64376565 MS.gene72499:CDS
AACATCGAAACATGTCGAAA+TGG 0.379750 4.1:+64375429 MS.gene72499:CDS
CAATGGTTTATTCGTCAATT+GGG 0.384937 4.1:+64375524 MS.gene72499:CDS
ATGTTTCTGGGTGAGGGGTT+TGG 0.391777 4.1:-64375110 None:intergenic
TCCAATGGGGTGGTCTCCAT+GGG 0.393186 4.1:+64376566 MS.gene72499:CDS
ATTTGCATGTTTCTGGGTGA+GGG 0.395262 4.1:-64375116 None:intergenic
ACATGTTTCGATGTTTGTAT+AGG 0.395429 4.1:-64375421 None:intergenic
ACATCGAAACATGTCGAAAT+GGG 0.395879 4.1:+64375430 MS.gene72499:CDS
GGTTCGAGAGAGGAAACTAT+TGG 0.398254 4.1:+64375620 MS.gene72499:CDS
GTGTTGAATTTCCCGACAAC+GGG 0.400235 4.1:-64376471 None:intergenic
TATTTGCATGTTTCTGGGTG+AGG 0.406728 4.1:-64375117 None:intergenic
AAATGGGTCATCTCGACCTT+GGG 0.407983 4.1:-64376391 None:intergenic
GGTGTCTTGGTAGCCTTTGA+AGG 0.411050 4.1:-64376220 None:intergenic
TGTAGTATTTGCATGTTTCT+GGG 0.427325 4.1:-64375122 None:intergenic
GGAAACTATTGGAACCTTCA+AGG 0.428925 4.1:+64375631 MS.gene72499:CDS
TGCGCCTTAATAACCCTTGA+AGG 0.430775 4.1:-64375645 None:intergenic
ATAATTTCGGACAAAATAGT+TGG 0.431028 4.1:+64375256 MS.gene72499:CDS
CATATCATTGTCGCGAAAGA+TGG 0.433386 4.1:+64375666 MS.gene72499:CDS
CTGAAGAAGGGTTTCCAAGA+TGG 0.434790 4.1:+64375592 MS.gene72499:CDS
AACTCTGGAGTATCTTTGCC+AGG 0.437542 4.1:-64376508 None:intergenic
GATAGTTTGGTCAGTCCAAT+GGG 0.442739 4.1:+64376552 MS.gene72499:CDS
TCTCGAACCGAAAACCATCT+TGG 0.449598 4.1:-64375606 None:intergenic
CATTGTCGCGAAAGATGGTT+CGG 0.450110 4.1:+64375671 MS.gene72499:CDS
CCAGATTTCAAGCCCGTTGT+CGG 0.457159 4.1:+64376459 MS.gene72499:CDS
TTAAACGTGATGGCCGTGTT+CGG 0.459371 4.1:+64375553 MS.gene72499:CDS
GGGCCGTGATCATGTTTGCT+TGG 0.459846 4.1:-64376371 None:intergenic
TGTGGTCATGAAATCATTCA+TGG 0.459935 4.1:+64376530 MS.gene72499:CDS
TATGGAGAGTACAAGAACTT+TGG 0.461429 4.1:+64376621 MS.gene72499:CDS
ACTGGTGTACCATTCACTTC+TGG 0.465852 4.1:+64376765 MS.gene72499:CDS
GAAATGGGTCATCTCGACCT+TGG 0.467978 4.1:-64376392 None:intergenic
CTTCTAGCAACAACATAGAT+TGG 0.468100 4.1:+64375079 MS.gene72499:CDS
CTTGTACGAAGGCCTTTAAA+CGG 0.468302 4.1:+64376348 MS.gene72499:CDS
AGAGTTGTGAATTGAAATTC+CGG 0.469872 4.1:-64376421 None:intergenic
TACCAAAATGACAATCATCA+TGG 0.471192 4.1:+64374981 None:intergenic
CAGATTTCAAGCCCGTTGTC+GGG 0.471943 4.1:+64376460 MS.gene72499:CDS
ACCTGCAGTGGCAGAACTAT+AGG 0.476593 4.1:-64375895 None:intergenic
ATAGAGAGAATATTGTGGTT+GGG 0.482521 4.1:+64375784 MS.gene72499:CDS
CAAGATGCTATAAATGCTGC+TGG 0.484980 4.1:+64375711 MS.gene72499:CDS
TTCTACCGTTGTGCCTTCAA+AGG 0.487774 4.1:+64376207 MS.gene72499:CDS
AATATTATGTTGGTTGGTGA+TGG 0.489232 4.1:+64375819 MS.gene72499:CDS
GGATAGTTTGGTCAGTCCAA+TGG 0.491721 4.1:+64376551 MS.gene72499:CDS
TATAGAGAGAATATTGTGGT+TGG 0.491777 4.1:+64375783 MS.gene72499:CDS
AATTCTCATTCATTTAGCCT+TGG 0.492233 4.1:+64375203 MS.gene72499:CDS
GTGAATGGTGATGCTTGAGC+TGG 0.493317 4.1:-64376702 None:intergenic
CCCTCCTTGCTGGCACCACA+TGG 0.495961 4.1:+64376733 MS.gene72499:CDS
AGGAGGGTAGCAACGGTGAA+TGG 0.500791 4.1:-64376717 None:intergenic
ACCAACGATGTAACTGAAGA+AGG 0.504546 4.1:+64375579 MS.gene72499:CDS
GAAACTATTGGAACCTTCAA+GGG 0.506160 4.1:+64375632 MS.gene72499:CDS
AGAAATAGAGTAAGATGGCC+TGG 0.507966 4.1:+64376660 MS.gene72499:CDS
TGGAGTATCTTTGCCAGGGC+CGG 0.508397 4.1:-64376503 None:intergenic
AGTAATAGAAATGTTCAAGC+TGG 0.512268 4.1:+64375864 MS.gene72499:CDS
AATGATAATATTATGTTGGT+TGG 0.513930 4.1:+64375813 MS.gene72499:CDS
TAATTTCGGACAAAATAGTT+GGG 0.519031 4.1:+64375257 MS.gene72499:CDS
CCCCATGGAGACCACCCCAT+TGG 0.520496 4.1:-64376567 None:intergenic
TGATTTCATGACCACAACTC+TGG 0.520918 4.1:-64376523 None:intergenic
GATGAATGATAATATTATGT+TGG 0.524956 4.1:+64375809 MS.gene72499:CDS
ACCTATAGTTCTGCCACTGC+AGG 0.525895 4.1:+64375894 MS.gene72499:CDS
TAAATGCTGCTGGGAGGAGA+AGG 0.527517 4.1:+64375721 MS.gene72499:CDS
AACATGTCGAAATGGGGCAT+TGG 0.527557 4.1:+64375437 MS.gene72499:CDS
CCCTTCTTCAGTTACATCGT+TGG 0.528030 4.1:-64375580 None:intergenic
CCATGTGGTGCCAGCAAGGA+GGG 0.530412 4.1:-64376733 None:intergenic
ACCACATGGTTACCTGCCAC+TGG 0.533118 4.1:+64376747 MS.gene72499:CDS
TTTGCATGTTTCTGGGTGAG+GGG 0.545288 4.1:-64375115 None:intergenic
ATGGGGTGGTCTCCATGGGG+AGG 0.545885 4.1:+64376570 MS.gene72499:CDS
AAGAATATCATTACCTGCAG+TGG 0.552651 4.1:-64375907 None:intergenic
AATTAAAGGCCAGAAGTGAA+TGG 0.553145 4.1:-64376774 None:intergenic
CAACAAGAAATAGAGTAAGA+TGG 0.559275 4.1:+64376655 MS.gene72499:CDS
CCAACGATGTAACTGAAGAA+GGG 0.560246 4.1:+64375580 MS.gene72499:CDS
CGTGAACCATGAGGGTGTCT+TGG 0.562307 4.1:-64376233 None:intergenic
TGGGAATTCGTTAAACGTGA+TGG 0.567493 4.1:+64375543 MS.gene72499:CDS
GGTGTTGAATTTCCCGACAA+CGG 0.567667 4.1:-64376472 None:intergenic
TGAATGGTGATGCTTGAGCT+GGG 0.570536 4.1:-64376701 None:intergenic
GCCAGCAAGGAGGGTAGCAA+CGG 0.571084 4.1:-64376724 None:intergenic
ACCAAAATGACAATCATCAT+GGG 0.572858 4.1:+64374982 None:intergenic
CATTACAACGGCTCTCTCCA+AGG 0.574210 4.1:-64375220 None:intergenic
CTAAATGAATGAGAATTTCG+CGG 0.574542 4.1:-64375197 None:intergenic
GTGAATGGTACACCAGTGGC+AGG 0.575851 4.1:-64376759 None:intergenic
ATGACACAAATCATTAGCAA+TGG 0.576321 4.1:+64375507 MS.gene72499:CDS
ATTGTCGCGAAAGATGGTTC+GGG 0.578990 4.1:+64375672 MS.gene72499:CDS
CATCATTCGATGCACAAACA+TGG 0.581027 4.1:+64375388 MS.gene72499:CDS
CATCGTTGGTGTACCGAACA+CGG 0.586715 4.1:-64375566 None:intergenic
GCAAACATGATCACGGCCCA+AGG 0.588675 4.1:+64376375 MS.gene72499:CDS
TTTGTTATACGTGTGAAGAG+AGG 0.590375 4.1:+64375756 MS.gene72499:CDS
CTAGCAACAACATAGATTGG+TGG 0.597852 4.1:+64375082 MS.gene72499:CDS
ACCACGTCGTGGTCAAGCAG+TGG 0.603947 4.1:+64376155 MS.gene72499:CDS
ACTGCATCATTCTTGTACGA+AGG 0.614280 4.1:+64376337 MS.gene72499:CDS
GTTTGGTCAGTCCAATGGGG+TGG 0.617183 4.1:+64376556 MS.gene72499:CDS
TGCACAAACATGGCTAAGCA+CGG 0.617923 4.1:+64375398 MS.gene72499:CDS
TGTTTATAGAGAGAATATTG+TGG 0.617943 4.1:+64375779 MS.gene72499:CDS
CCAATGGGGTGGTCTCCATG+GGG 0.626856 4.1:+64376567 MS.gene72499:CDS
CATCGAAACATGTCGAAATG+GGG 0.628859 4.1:+64375431 MS.gene72499:CDS
GTAACCATGTGGTGCCAGCA+AGG 0.628912 4.1:-64376737 None:intergenic
GATGACCCATTTCAAAACAC+CGG 0.632618 4.1:+64376402 MS.gene72499:CDS
GCAAAGATACTCCAGAGTTG+TGG 0.635768 4.1:+64376512 MS.gene72499:CDS
CGGCCAAGCAAACATGATCA+CGG 0.638383 4.1:+64376368 MS.gene72499:CDS
AAGATGCTATAAATGCTGCT+GGG 0.644279 4.1:+64375712 MS.gene72499:CDS
GAGTACAAGAACTTTGGACC+TGG 0.646666 4.1:+64376627 MS.gene72499:CDS
GTTGCCAAAGATGAAGTCAA+CGG 0.646875 4.1:-64376295 None:intergenic
AGGCTACCAAGACACCCTCA+TGG 0.652925 4.1:+64376227 MS.gene72499:CDS
CACAACGGTAGAAAACAGAT+AGG 0.659204 4.1:-64376197 None:intergenic
ACTCTGGAGTATCTTTGCCA+GGG 0.660708 4.1:-64376507 None:intergenic
ACCATGTGGTGCCAGCAAGG+AGG 0.665765 4.1:-64376734 None:intergenic
GCAAAATTAGTACCTCCCCA+TGG 0.681295 4.1:-64376582 None:intergenic
AACACGGCTGGACCACGTCG+TGG 0.685767 4.1:+64376144 MS.gene72499:CDS
ATGCTATAAATGCTGCTGGG+AGG 0.686085 4.1:+64375715 MS.gene72499:CDS
GGTAGCCTTTGAAGGCACAA+CGG 0.688392 4.1:-64376212 None:intergenic
AGAAGTGAATGGTACACCAG+TGG 0.693790 4.1:-64376763 None:intergenic
GCCACTGCTTGACCACGACG+TGG 0.699801 4.1:-64376156 None:intergenic
GACGTTGTGCGTGAACCATG+AGG 0.708175 4.1:-64376242 None:intergenic
GTAGAAAACAGATAGGTCAG+AGG 0.708320 4.1:-64376190 None:intergenic
ACGTTGTGCGTGAACCATGA+GGG 0.708924 4.1:-64376241 None:intergenic
ACCAGTGGCAGGTAACCATG+TGG 0.747350 4.1:-64376748 None:intergenic
ATAGTTTGGTCAGTCCAATG+GGG 0.792431 4.1:+64376553 MS.gene72499:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATGTTTATAACTTTCATAAT+TGG - chr4.1:64375489-64375508 None:intergenic 15.0%
!!! AAAATTTTTTTGTGTACTAT+AGG + chr4.1:64376078-64376097 MS.gene72499:intron 15.0%
!!! TCAACTAAATTTTTTTCTTT+AGG + chr4.1:64375956-64375975 MS.gene72499:intron 15.0%
!! AATGATAATATTATGTTGGT+TGG + chr4.1:64375813-64375832 MS.gene72499:CDS 20.0%
!! ATATATAACAATAAGGAAAG+AGG - chr4.1:64376014-64376033 None:intergenic 20.0%
!! GATGAATGATAATATTATGT+TGG + chr4.1:64375809-64375828 MS.gene72499:CDS 20.0%
!! GTTAGAGATATATAACAATA+AGG - chr4.1:64376021-64376040 None:intergenic 20.0%
!!! ATAATTGGTACAATGAATTT+TGG - chr4.1:64375474-64375493 None:intergenic 20.0%
! ATAATTTCGGACAAAATAGT+TGG + chr4.1:64375256-64375275 MS.gene72499:CDS 25.0%
! TAATTTCGGACAAAATAGTT+GGG + chr4.1:64375257-64375276 MS.gene72499:CDS 25.0%
! TGTTTATAGAGAGAATATTG+TGG + chr4.1:64375779-64375798 MS.gene72499:CDS 25.0%
!!! ATGAACATGATTTTTTCTTG+TGG - chr4.1:64375371-64375390 None:intergenic 25.0%
!!! GTTTTTGTTGTGATAGTAAT+GGG - chr4.1:64375157-64375176 None:intergenic 25.0%
!!! TCTTAGCTATTTTTCCTTAT+GGG + chr4.1:64375926-64375945 MS.gene72499:intron 25.0%
!!! TGACATTACTTTTGAAAACA+CGG + chr4.1:64376128-64376147 MS.gene72499:CDS 25.0%
!!! TGTTTTTGTTGTGATAGTAA+TGG - chr4.1:64375158-64375177 None:intergenic 25.0%
!!! TTCTTAGCTATTTTTCCTTA+TGG + chr4.1:64375925-64375944 MS.gene72499:intron 25.0%
AATTCACAACTCTCAGATTA+GGG + chr4.1:64376431-64376450 MS.gene72499:CDS 30.0%
AATTCTCATTCATTTAGCCT+TGG + chr4.1:64375203-64375222 MS.gene72499:CDS 30.0%
ACATGTTTCGATGTTTGTAT+AGG - chr4.1:64375424-64375443 None:intergenic 30.0%
AGAGTTGTGAATTGAAATTC+CGG - chr4.1:64376424-64376443 None:intergenic 30.0%
AGTAATAGAAATGTTCAAGC+TGG + chr4.1:64375864-64375883 MS.gene72499:CDS 30.0%
ATAGAGAGAATATTGTGGTT+GGG + chr4.1:64375784-64375803 MS.gene72499:CDS 30.0%
ATGACACAAATCATTAGCAA+TGG + chr4.1:64375507-64375526 MS.gene72499:CDS 30.0%
ATGTAGTATTTGCATGTTTC+TGG - chr4.1:64375126-64375145 None:intergenic 30.0%
CAACAAGAAATAGAGTAAGA+TGG + chr4.1:64376655-64376674 MS.gene72499:CDS 30.0%
CAATGGTTTATTCGTCAATT+GGG + chr4.1:64375524-64375543 MS.gene72499:CDS 30.0%
CATCTTGTACTCTCTTAAAA+TGG - chr4.1:64375698-64375717 None:intergenic 30.0%
CTAAATGAATGAGAATTTCG+CGG - chr4.1:64375200-64375219 None:intergenic 30.0%
GAAATCATTCATGGATAGTT+TGG + chr4.1:64376539-64376558 MS.gene72499:CDS 30.0%
TACAGAGAATGTTACATCTA+TGG + chr4.1:64376270-64376289 MS.gene72499:CDS 30.0%
TATAGAGAGAATATTGTGGT+TGG + chr4.1:64375783-64375802 MS.gene72499:CDS 30.0%
TGTAGTATTTGCATGTTTCT+GGG - chr4.1:64375125-64375144 None:intergenic 30.0%
TTGTGTACTATAGGAATTGA+TGG + chr4.1:64376087-64376106 MS.gene72499:intron 30.0%
! CTTTTCATAACATGATAACC+AGG - chr4.1:64376681-64376700 None:intergenic 30.0%
! TGATCTTCTTTTTGATGAAG+TGG - chr4.1:64375055-64375074 None:intergenic 30.0%
!! AATATTATGTTGGTTGGTGA+TGG + chr4.1:64375819-64375838 MS.gene72499:CDS 30.0%
!!! AAATTCCGGTGTTTTGAAAT+GGG - chr4.1:64376410-64376429 None:intergenic 30.0%
!!! AACATGATTTTTTCTTGTGG+AGG - chr4.1:64375368-64375387 None:intergenic 30.0%
!!! CTTAGCTATTTTTCCTTATG+GGG + chr4.1:64375927-64375946 MS.gene72499:intron 30.0%
!!! TGACACTGATTTTTTCTTCT+GGG - chr4.1:64375286-64375305 None:intergenic 30.0%
AACATCGAAACATGTCGAAA+TGG + chr4.1:64375429-64375448 MS.gene72499:CDS 35.0%
AAGAATATCATTACCTGCAG+TGG - chr4.1:64375910-64375929 None:intergenic 35.0%
AAGATGCTATAAATGCTGCT+GGG + chr4.1:64375712-64375731 MS.gene72499:CDS 35.0%
ACATCGAAACATGTCGAAAT+GGG + chr4.1:64375430-64375449 MS.gene72499:CDS 35.0%
ATAAGGAAAGAGGAGAAGAA+TGG - chr4.1:64376004-64376023 None:intergenic 35.0%
CAATTCACAACTCTCAGATT+AGG + chr4.1:64376430-64376449 MS.gene72499:CDS 35.0%
CGAAAAATGGCGCATAATTT+CGG + chr4.1:64375243-64375262 MS.gene72499:CDS 35.0%
CTAAAAATGTACGGTTGAGA+TGG - chr4.1:64375344-64375363 None:intergenic 35.0%
CTTCTAGCAACAACATAGAT+TGG + chr4.1:64375079-64375098 MS.gene72499:CDS 35.0%
GAAACTATTGGAACCTTCAA+GGG + chr4.1:64375632-64375651 MS.gene72499:CDS 35.0%
GCAATGGTTTATTCGTCAAT+TGG + chr4.1:64375523-64375542 MS.gene72499:CDS 35.0%
TAGTTGAATTTGACCCCATA+AGG - chr4.1:64375943-64375962 None:intergenic 35.0%
TGTGGAGGTCTAAAAATGTA+CGG - chr4.1:64375353-64375372 None:intergenic 35.0%
TGTGGTCATGAAATCATTCA+TGG + chr4.1:64376530-64376549 MS.gene72499:CDS 35.0%
! GGGAAATTCAACACCTTTTT+GGG + chr4.1:64376480-64376499 MS.gene72499:CDS 35.0%
! GTACAAGAATGATGCAGTTT+TGG - chr4.1:64376335-64376354 None:intergenic 35.0%
! TATGGAGAGTACAAGAACTT+TGG + chr4.1:64376621-64376640 MS.gene72499:CDS 35.0%
! TTTGTTATACGTGTGAAGAG+AGG + chr4.1:64375756-64375775 MS.gene72499:CDS 35.0%
!! ATTACTTTTGAAAACACGGC+TGG + chr4.1:64376132-64376151 MS.gene72499:CDS 35.0%
!! CTATTTCTTGTTGATGATCC+AGG - chr4.1:64376648-64376667 None:intergenic 35.0%
!!! GAAATTCCGGTGTTTTGAAA+TGG - chr4.1:64376411-64376430 None:intergenic 35.0%
!!! GATGCAGTTTTGGAAAACTA+CGG - chr4.1:64376325-64376344 None:intergenic 35.0%
!!! GTGACACTGATTTTTTCTTC+TGG - chr4.1:64375287-64375306 None:intergenic 35.0%
ACCAACGATGTAACTGAAGA+AGG + chr4.1:64375579-64375598 MS.gene72499:CDS 40.0%
ACTGCATCATTCTTGTACGA+AGG + chr4.1:64376337-64376356 MS.gene72499:CDS 40.0%
AGAAATAGAGTAAGATGGCC+TGG + chr4.1:64376660-64376679 MS.gene72499:CDS 40.0%
AGCCGTTGTAATGCGAAAAA+TGG + chr4.1:64375230-64375249 MS.gene72499:CDS 40.0%
ATAGTTTGGTCAGTCCAATG+GGG + chr4.1:64376553-64376572 MS.gene72499:CDS 40.0%
ATGTTTGCTTGGCCGTTTAA+AGG - chr4.1:64376363-64376382 None:intergenic 40.0%
CAAGATGCTATAAATGCTGC+TGG + chr4.1:64375711-64375730 MS.gene72499:CDS 40.0%
CACAACGGTAGAAAACAGAT+AGG - chr4.1:64376200-64376219 None:intergenic 40.0%
CATATCATTGTCGCGAAAGA+TGG + chr4.1:64375666-64375685 MS.gene72499:CDS 40.0%
CATCATTCGATGCACAAACA+TGG + chr4.1:64375388-64375407 MS.gene72499:CDS 40.0%
CATCGAAACATGTCGAAATG+GGG + chr4.1:64375431-64375450 MS.gene72499:CDS 40.0%
CCAACGATGTAACTGAAGAA+GGG + chr4.1:64375580-64375599 MS.gene72499:CDS 40.0%
CTAGCAACAACATAGATTGG+TGG + chr4.1:64375082-64375101 MS.gene72499:CDS 40.0%
CTTGTACGAAGGCCTTTAAA+CGG + chr4.1:64376348-64376367 MS.gene72499:CDS 40.0%
GATAGTTTGGTCAGTCCAAT+GGG + chr4.1:64376552-64376571 MS.gene72499:CDS 40.0%
GATGACCCATTTCAAAACAC+CGG + chr4.1:64376402-64376421 MS.gene72499:CDS 40.0%
GGAAACTATTGGAACCTTCA+AGG + chr4.1:64375631-64375650 MS.gene72499:CDS 40.0%
GGAACCTTCAAGGGTTATTA+AGG + chr4.1:64375641-64375660 MS.gene72499:CDS 40.0%
GTAGAAAACAGATAGGTCAG+AGG - chr4.1:64376193-64376212 None:intergenic 40.0%
GTTGCCAAAGATGAAGTCAA+CGG - chr4.1:64376298-64376317 None:intergenic 40.0%
TGATTTCATGACCACAACTC+TGG - chr4.1:64376526-64376545 None:intergenic 40.0%
TGGGAATTCGTTAAACGTGA+TGG + chr4.1:64375543-64375562 MS.gene72499:CDS 40.0%
! AGGGTTTCCAAGATGGTTTT+CGG + chr4.1:64375599-64375618 MS.gene72499:CDS 40.0%
! CGGGAAATTCAACACCTTTT+TGG + chr4.1:64376479-64376498 MS.gene72499:CDS 40.0%
! GCACAAAGCACTCTATTCTA+TGG + chr4.1:64376603-64376622 MS.gene72499:CDS 40.0%
!! AATTCAACACCTTTTTGGGC+CGG + chr4.1:64376484-64376503 MS.gene72499:CDS 40.0%
!! ATTTGCATGTTTCTGGGTGA+GGG - chr4.1:64375119-64375138 None:intergenic 40.0%
!! TATTTGCATGTTTCTGGGTG+AGG - chr4.1:64375120-64375139 None:intergenic 40.0%
!!! CGCCATTTTTCGCATTACAA+CGG - chr4.1:64375235-64375254 None:intergenic 40.0%
AAATGGGTCATCTCGACCTT+GGG - chr4.1:64376394-64376413 None:intergenic 45.0%
AACATGTCGAAATGGGGCAT+TGG + chr4.1:64375437-64375456 MS.gene72499:CDS 45.0%
ACTGGTGTACCATTCACTTC+TGG + chr4.1:64376765-64376784 MS.gene72499:CDS 45.0%
AGAAGTGAATGGTACACCAG+TGG - chr4.1:64376766-64376785 None:intergenic 45.0%
ATGCTATAAATGCTGCTGGG+AGG + chr4.1:64375715-64375734 MS.gene72499:CDS 45.0%
ATTGTCGCGAAAGATGGTTC+GGG + chr4.1:64375672-64375691 MS.gene72499:CDS 45.0%
CATTGTCGCGAAAGATGGTT+CGG + chr4.1:64375671-64375690 MS.gene72499:CDS 45.0%
CCCTTCTTCAGTTACATCGT+TGG - chr4.1:64375583-64375602 None:intergenic 45.0%
CTGAAGAAGGGTTTCCAAGA+TGG + chr4.1:64375592-64375611 MS.gene72499:CDS 45.0%
GAGTACAAGAACTTTGGACC+TGG + chr4.1:64376627-64376646 MS.gene72499:CDS 45.0%
GCAAAATTAGTACCTCCCCA+TGG - chr4.1:64376585-64376604 None:intergenic 45.0%
GCAAAGATACTCCAGAGTTG+TGG + chr4.1:64376512-64376531 MS.gene72499:CDS 45.0%
GGATAGTTTGGTCAGTCCAA+TGG + chr4.1:64376551-64376570 MS.gene72499:CDS 45.0%
GGTTCGAGAGAGGAAACTAT+TGG + chr4.1:64375620-64375639 MS.gene72499:CDS 45.0%
GTGTTGAATTTCCCGACAAC+GGG - chr4.1:64376474-64376493 None:intergenic 45.0%
TCTCGAACCGAAAACCATCT+TGG - chr4.1:64375609-64375628 None:intergenic 45.0%
TGCACAAACATGGCTAAGCA+CGG + chr4.1:64375398-64375417 MS.gene72499:CDS 45.0%
TGCGCCTTAATAACCCTTGA+AGG - chr4.1:64375648-64375667 None:intergenic 45.0%
TTAAACGTGATGGCCGTGTT+CGG + chr4.1:64375553-64375572 MS.gene72499:CDS 45.0%
TTCTACCGTTGTGCCTTCAA+AGG + chr4.1:64376207-64376226 MS.gene72499:CDS 45.0%
! GGTGTTGAATTTCCCGACAA+CGG - chr4.1:64376475-64376494 None:intergenic 45.0%
! TGAATGGTGATGCTTGAGCT+GGG - chr4.1:64376704-64376723 None:intergenic 45.0%
!! AACTCTGGAGTATCTTTGCC+AGG - chr4.1:64376511-64376530 None:intergenic 45.0%
!! ACTCTGGAGTATCTTTGCCA+GGG - chr4.1:64376510-64376529 None:intergenic 45.0%
!! TTTGCATGTTTCTGGGTGAG+GGG - chr4.1:64375118-64375137 None:intergenic 45.0%
ACCTGCAGTGGCAGAACTAT+AGG - chr4.1:64375898-64375917 None:intergenic 50.0%
ACGTTGTGCGTGAACCATGA+GGG - chr4.1:64376244-64376263 None:intergenic 50.0%
CAGATTTCAAGCCCGTTGTC+GGG + chr4.1:64376460-64376479 MS.gene72499:CDS 50.0%
CATCGTTGGTGTACCGAACA+CGG - chr4.1:64375569-64375588 None:intergenic 50.0%
CATTACAACGGCTCTCTCCA+AGG - chr4.1:64375223-64375242 None:intergenic 50.0%
CCAGATTTCAAGCCCGTTGT+CGG + chr4.1:64376459-64376478 MS.gene72499:CDS 50.0%
CGGCCAAGCAAACATGATCA+CGG + chr4.1:64376368-64376387 MS.gene72499:CDS 50.0%
GAAATGGGTCATCTCGACCT+TGG - chr4.1:64376395-64376414 None:intergenic 50.0%
TAAATGCTGCTGGGAGGAGA+AGG + chr4.1:64375721-64375740 MS.gene72499:CDS 50.0%
! ACCTATAGTTCTGCCACTGC+AGG + chr4.1:64375894-64375913 MS.gene72499:CDS 50.0%
! GGTAGCCTTTGAAGGCACAA+CGG - chr4.1:64376215-64376234 None:intergenic 50.0%
! GGTGTCTTGGTAGCCTTTGA+AGG - chr4.1:64376223-64376242 None:intergenic 50.0%
! GTGAATGGTGATGCTTGAGC+TGG - chr4.1:64376705-64376724 None:intergenic 50.0%
!! ATGTTTCTGGGTGAGGGGTT+TGG - chr4.1:64375113-64375132 None:intergenic 50.0%
!! GATGGTTTTCGGTTCGAGAG+AGG + chr4.1:64375610-64375629 MS.gene72499:CDS 50.0%
!! GGCACCGTTGACTTCATCTT+TGG + chr4.1:64376291-64376310 MS.gene72499:CDS 50.0%
ACCACATGGTTACCTGCCAC+TGG + chr4.1:64376747-64376766 MS.gene72499:CDS 55.0%
ACCAGTGGCAGGTAACCATG+TGG - chr4.1:64376751-64376770 None:intergenic 55.0%
AGGAGGGTAGCAACGGTGAA+TGG - chr4.1:64376720-64376739 None:intergenic 55.0%
AGGCTACCAAGACACCCTCA+TGG + chr4.1:64376227-64376246 MS.gene72499:CDS 55.0%
GACGTTGTGCGTGAACCATG+AGG - chr4.1:64376245-64376264 None:intergenic 55.0%
GCAAACATGATCACGGCCCA+AGG + chr4.1:64376375-64376394 MS.gene72499:CDS 55.0%
GGGCCGTGATCATGTTTGCT+TGG - chr4.1:64376374-64376393 None:intergenic 55.0%
GTGAATGGTACACCAGTGGC+AGG - chr4.1:64376762-64376781 None:intergenic 55.0%
GTTTGGTCAGTCCAATGGGG+TGG + chr4.1:64376556-64376575 MS.gene72499:CDS 55.0%
TCCAATGGGGTGGTCTCCAT+GGG + chr4.1:64376566-64376585 MS.gene72499:CDS 55.0%
! CCGACAACGGGCTTGAAATC+TGG - chr4.1:64376462-64376481 None:intergenic 55.0%
! GTAACCATGTGGTGCCAGCA+AGG - chr4.1:64376740-64376759 None:intergenic 55.0%
! TGGAGTATCTTTGCCAGGGC+CGG - chr4.1:64376506-64376525 None:intergenic 55.0%
!! CGTGAACCATGAGGGTGTCT+TGG - chr4.1:64376236-64376255 None:intergenic 55.0%
ACCACGTCGTGGTCAAGCAG+TGG + chr4.1:64376155-64376174 MS.gene72499:CDS 60.0%
ACCGTTGCTACCCTCCTTGC+TGG + chr4.1:64376723-64376742 MS.gene72499:CDS 60.0%
CCAATGGGGTGGTCTCCATG+GGG + chr4.1:64376567-64376586 MS.gene72499:CDS 60.0%
GCCAGCAAGGAGGGTAGCAA+CGG - chr4.1:64376727-64376746 None:intergenic 60.0%
GTCCAATGGGGTGGTCTCCA+TGG + chr4.1:64376565-64376584 MS.gene72499:CDS 60.0%
! ACCATGTGGTGCCAGCAAGG+AGG - chr4.1:64376737-64376756 None:intergenic 60.0%
! CCATGTGGTGCCAGCAAGGA+GGG - chr4.1:64376736-64376755 None:intergenic 60.0%
AACACGGCTGGACCACGTCG+TGG + chr4.1:64376144-64376163 MS.gene72499:CDS 65.0%
ATGGGGTGGTCTCCATGGGG+AGG + chr4.1:64376570-64376589 MS.gene72499:CDS 65.0%
CCCCATGGAGACCACCCCAT+TGG - chr4.1:64376570-64376589 None:intergenic 65.0%
CCCTCCTTGCTGGCACCACA+TGG + chr4.1:64376733-64376752 MS.gene72499:CDS 65.0%
GCCACTGCTTGACCACGACG+TGG - chr4.1:64376159-64376178 None:intergenic 65.0%
TTGCCAGGGCCGGCCCAAAA+AGG - chr4.1:64376496-64376515 None:intergenic 65.0%
!! ACACCTTTTTGGGCCGGCCC+TGG + chr4.1:64376490-64376509 MS.gene72499:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 64374988 64376794 64374988 ID=MS.gene72499
chr4.1 mRNA 64374988 64376794 64374988 ID=MS.gene72499.t1;Parent=MS.gene72499
chr4.1 exon 64374988 64375915 64374988 ID=MS.gene72499.t1.exon1;Parent=MS.gene72499.t1
chr4.1 CDS 64374988 64375915 64374988 ID=cds.MS.gene72499.t1;Parent=MS.gene72499.t1
chr4.1 exon 64376100 64376794 64376100 ID=MS.gene72499.t1.exon2;Parent=MS.gene72499.t1
chr4.1 CDS 64376100 64376794 64376100 ID=cds.MS.gene72499.t1;Parent=MS.gene72499.t1
Gene Sequence

>MS.gene72499

ATGACAATCATCATGGGAAAGATATTGTTAACACTCTTATTGTTATCATCAATTTTCTCAATAGCCACTTCATCAAAAAGAAGATCAAGATCTTCTAGCAACAACATAGATTGGTGGTGTAACCAAACCCCTCACCCAGAAACATGCAAATACTACATAAAACAAACCCATTACTATCACAACAAAAACATCAAACACAAATCAAAATTCCGCGAAATTCTCATTCATTTAGCCTTGGAGAGAGCCGTTGTAATGCGAAAAATGGCGCATAATTTCGGACAAAATAGTTGGGTCACCCAGAAGAAAAAATCAGTGTCACGTGATTGTTTAAAACTCTTCGACAACACAGTTTTCCATCTCAACCGTACATTTTTAGACCTCCACAAGAAAAAATCATGTTCATCATTCGATGCACAAACATGGCTAAGCACGGCCTATACAAACATCGAAACATGTCGAAATGGGGCATTGGAATTGAACATTCCAAAATTCATTGTACCAATTATGAAAGTTATAAACATGACACAAATCATTAGCAATGGTTTATTCGTCAATTGGGAATTCGTTAAACGTGATGGCCGTGTTCGGTACACCAACGATGTAACTGAAGAAGGGTTTCCAAGATGGTTTTCGGTTCGAGAGAGGAAACTATTGGAACCTTCAAGGGTTATTAAGGCGCATATCATTGTCGCGAAAGATGGTTCGGGCCATTTTAAGAGAGTACAAGATGCTATAAATGCTGCTGGGAGGAGAAGGTTTAAAACAAGATTTGTTATACGTGTGAAGAGAGGTGTTTATAGAGAGAATATTGTGGTTGGGAAGATGAATGATAATATTATGTTGGTTGGTGATGGAAGTAGATATACAGTGATAACAAGTAATAGAAATGTTCAAGCTGGTTTTACTACCTATAGTTCTGCCACTGCAGGTAATGATATTCTTAGCTATTTTTCCTTATGGGGTCAAATTCAACTAAATTTTTTTCTTTAGGAATATGAATTCTATAATTCTATCCATTCTTCTCCTCTTTCCTTATTGTTATATATCTCTAACTATTATTATCATAACAAATTCAAATCAACACATTCTAAAAATTTTTTTGTGTACTATAGGAATTGATGGTCTTCACTTCATTGCACGTGACATTACTTTTGAAAACACGGCTGGACCACGTCGTGGTCAAGCAGTGGCGCTTCGATCAGCCTCTGACCTATCTGTTTTCTACCGTTGTGCCTTCAAAGGCTACCAAGACACCCTCATGGTTCACGCACAACGTCAATTTTACAGAGAATGTTACATCTATGGCACCGTTGACTTCATCTTTGGCAACGCAGCCGTAGTTTTCCAAAACTGCATCATTCTTGTACGAAGGCCTTTAAACGGCCAAGCAAACATGATCACGGCCCAAGGTCGAGATGACCCATTTCAAAACACCGGAATTTCAATTCACAACTCTCAGATTAGGGCTGCTCCAGATTTCAAGCCCGTTGTCGGGAAATTCAACACCTTTTTGGGCCGGCCCTGGCAAAGATACTCCAGAGTTGTGGTCATGAAATCATTCATGGATAGTTTGGTCAGTCCAATGGGGTGGTCTCCATGGGGAGGTACTAATTTTGCACAAAGCACTCTATTCTATGGAGAGTACAAGAACTTTGGACCTGGATCATCAACAAGAAATAGAGTAAGATGGCCTGGTTATCATGTTATGAAAAGCCCAGCTCAAGCATCACCATTCACCGTTGCTACCCTCCTTGCTGGCACCACATGGTTACCTGCCACTGGTGTACCATTCACTTCTGGCCTTTAA
Protein sequence

>MS.gene72499.t1

MTIIMGKILLTLLLLSSIFSIATSSKRRSRSSSNNIDWWCNQTPHPETCKYYIKQTHYYHNKNIKHKSKFREILIHLALERAVVMRKMAHNFGQNSWVTQKKKSVSRDCLKLFDNTVFHLNRTFLDLHKKKSCSSFDAQTWLSTAYTNIETCRNGALELNIPKFIVPIMKVINMTQIISNGLFVNWEFVKRDGRVRYTNDVTEEGFPRWFSVRERKLLEPSRVIKAHIIVAKDGSGHFKRVQDAINAAGRRRFKTRFVIRVKRGVYRENIVVGKMNDNIMLVGDGSRYTVITSNRNVQAGFTTYSSATAGIDGLHFIARDITFENTAGPRRGQAVALRSASDLSVFYRCAFKGYQDTLMVHAQRQFYRECYIYGTVDFIFGNAAVVFQNCIILVRRPLNGQANMITAQGRDDPFQNTGISIHNSQIRAAPDFKPVVGKFNTFLGRPWQRYSRVVVMKSFMDSLVSPMGWSPWGGTNFAQSTLFYGEYKNFGPGSSTRNRVRWPGYHVMKSPAQASPFTVATLLAGTTWLPATGVPFTSGL