AlfalfaGEDB | MS.gene47008

M. sativa cultivar XinJiangDaYe / MS.gene47008

KEGG: K21777
GO: -
PFAM: PF00134.26

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene47008.t1	XP_003617201.1	73.7	167	44	0	5	171	88	254	5.40E-59	237.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene47008.t1	P30183	52.1	169	73	3	5	171	92	254	1.3e-37	157.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene47008.t1	G7KCT9	73.7	167	44	0	5	171	88	254	3.9e-59	237.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene61084	MS.gene47008	PPI
MS.gene47008	MS.gene028366	PPI
MS.gene79044	MS.gene47008	PPI
MS.gene82304	MS.gene47008	PPI
MS.gene019336	MS.gene47008	PPI
MS.gene55900	MS.gene47008	PPI
MS.gene04541	MS.gene47008	PPI
MS.gene016544	MS.gene47008	PPI
MS.gene47008	MS.gene00478	PPI
MS.gene00474	MS.gene47008	PPI
MS.gene016703	MS.gene47008	PPI
MS.gene36424	MS.gene47008	PPI
MS.gene47008	MS.gene55782	PPI
MS.gene28739	MS.gene47008	PPI
MS.gene67884	MS.gene47008	PPI
MS.gene47008	MS.gene044085	PPI
MS.gene60448	MS.gene47008	PPI
MS.gene47008	MS.gene00063	PPI
MS.gene04501	MS.gene47008	PPI
MS.gene47008	MS.gene06057	PPI
MS.gene47008	MS.gene048913	PPI
MS.gene23576	MS.gene47008	PPI
MS.gene35086	MS.gene47008	PPI
MS.gene88440	MS.gene47008	PPI
MS.gene47008	MS.gene46805	PPI
MS.gene47008	MS.gene04020	PPI
MS.gene47008	MS.gene063809	PPI
MS.gene47008	MS.gene24450	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene47008.t1	MTR_5g088980	73.653	167	44	0	5	171	88	254	4.72e-83	251
MS.gene47008.t1	MTR_7g089080	51.190	168	78	2	5	171	124	288	1.45e-48	168
MS.gene47008.t1	MTR_5g023790	46.018	113	60	1	60	171	150	262	9.38e-30	112
MS.gene47008.t1	MTR_5g023790	46.018	113	60	1	60	171	150	262	1.33e-29	112
MS.gene47008.t1	MTR_5g023790	46.018	113	60	1	60	171	150	262	1.85e-29	112
MS.gene47008.t1	MTR_8g074000	44.545	110	60	1	63	171	157	266	1.42e-27	107
MS.gene47008.t1	MTR_1g011470	40.945	127	68	4	47	171	131	252	6.77e-22	91.3
MS.gene47008.t1	MTR_3g088415	39.130	138	72	4	45	171	195	331	1.01e-21	90.9
MS.gene47008.t1	MTR_3g088415	39.130	138	72	4	45	171	195	331	1.44e-21	90.5
MS.gene47008.t1	MTR_3g088415	39.130	138	72	4	45	171	195	331	1.54e-21	90.9
MS.gene47008.t1	MTR_3g107970	42.593	108	59	3	66	171	198	304	8.19e-21	88.6
MS.gene47008.t1	MTR_2g102520	40.000	130	70	4	50	171	177	306	1.92e-19	84.7
MS.gene47008.t1	MTR_3g102520	40.566	106	59	2	70	171	79	184	2.32e-18	81.3
MS.gene47008.t1	MTR_3g102530	40.566	106	59	1	70	171	72	177	8.56e-18	79.3
MS.gene47008.t1	MTR_2g102550	38.519	135	77	5	42	171	125	258	1.39e-17	79.3
MS.gene47008.t1	MTR_1g018680	41.748	103	55	3	73	171	83	184	3.29e-17	77.8
MS.gene47008.t1	MTR_3g102510	44.565	92	49	2	82	171	74	165	5.87e-17	77.0
MS.gene47008.t1	MTR_3g060770	37.719	114	63	3	65	173	13	123	1.09e-16	75.9
MS.gene47008.t1	MTR_3g088405	37.963	108	64	3	66	171	228	334	1.21e-16	76.6
MS.gene47008.t1	MTR_3g061680	40.196	102	55	2	75	173	15	113	2.43e-16	74.7
MS.gene47008.t1	MTR_3g060400	40.206	97	52	2	80	173	26	119	1.38e-15	72.8
MS.gene47008.t1	MTR_3g460990	40.206	97	52	2	80	173	26	119	1.38e-15	72.8
MS.gene47008.t1	MTR_5g011900	37.500	112	69	1	62	172	50	161	4.36e-15	70.9
MS.gene47008.t1	MTR_3g060820	39.175	97	53	2	80	173	20	113	5.86e-15	70.9
MS.gene47008.t1	MTR_5g088940	55.738	61	8	1	103	163	1	42	2.37e-14	64.7
MS.gene47008.t1	MTR_2g005960	32.800	125	69	4	62	171	102	226	3.78e-13	65.5
MS.gene47008.t1	MTR_6g045270	38.542	96	53	2	81	173	45	137	5.35e-13	65.9
MS.gene47008.t1	MTR_3g102560	47.222	72	37	1	101	171	86	157	5.92e-12	62.8
MS.gene47008.t1	MTR_3g060410	44.118	68	35	1	109	173	131	198	8.97e-11	59.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene47008.t1	AT4G37490	59.843	127	49	1	47	171	128	254	1.42e-46	157
MS.gene47008.t1	AT2G26760	49.390	164	73	3	8	171	66	219	6.44e-46	155
MS.gene47008.t1	AT5G06150	58.730	126	52	0	46	171	146	271	1.26e-45	152
MS.gene47008.t1	AT5G06150	58.730	126	52	0	46	171	146	271	1.60e-44	152
MS.gene47008.t1	AT3G11520	46.626	163	68	3	9	171	105	248	6.72e-38	134
MS.gene47008.t1	AT3G11520	53.968	126	52	1	46	171	129	248	7.77e-38	134
MS.gene47008.t1	AT1G34460	42.208	154	77	2	8	158	204	348	8.63e-32	119
MS.gene47008.t1	AT1G34460	42.208	154	77	2	8	158	204	348	8.63e-32	119
MS.gene47008.t1	AT1G34460	42.208	154	77	2	8	158	204	348	8.63e-32	119
MS.gene47008.t1	AT1G34460	42.208	154	77	2	8	158	204	348	8.63e-32	119
MS.gene47008.t1	AT1G34460	42.208	154	77	2	8	158	204	348	8.87e-32	119
MS.gene47008.t1	AT1G34460	42.208	154	77	2	8	158	190	334	9.04e-32	119
MS.gene47008.t1	AT1G34460	41.722	151	79	1	8	158	204	345	1.09e-31	119
MS.gene47008.t1	AT1G34460	41.722	151	79	1	8	158	204	345	1.09e-31	119
MS.gene47008.t1	AT1G34460	42.208	154	77	2	8	158	204	348	1.13e-31	119
MS.gene47008.t1	AT1G76310	49.541	109	53	2	65	171	160	268	1.19e-30	114
MS.gene47008.t1	AT1G76310	49.541	109	53	2	65	171	160	268	4.39e-30	113
MS.gene47008.t1	AT1G76310	49.541	109	53	2	65	171	160	268	4.97e-30	114
MS.gene47008.t1	AT1G76310	49.541	109	53	2	65	171	160	268	5.83e-30	114
MS.gene47008.t1	AT1G20610	44.444	108	59	1	65	171	160	267	9.16e-29	110
MS.gene47008.t1	AT1G16330	42.574	101	57	1	72	171	385	485	5.97e-24	97.8
MS.gene47008.t1	AT1G44110	42.857	112	61	3	62	171	175	285	3.57e-23	95.1
MS.gene47008.t1	AT4G35620	50.926	108	52	1	65	171	155	262	9.99e-23	93.6
MS.gene47008.t1	AT4G35620	50.926	108	52	1	65	171	155	262	1.14e-22	93.6
MS.gene47008.t1	AT1G77390	43.119	109	59	3	65	171	162	269	1.29e-21	90.9
MS.gene47008.t1	AT1G47230	33.140	172	89	4	4	171	39	188	1.50e-21	90.1
MS.gene47008.t1	AT2G17620	46.491	114	60	1	59	171	148	261	4.15e-21	89.4
MS.gene47008.t1	AT1G47220	45.045	111	56	3	67	173	42	151	5.77e-21	87.8
MS.gene47008.t1	AT5G43080	37.500	152	91	4	23	171	28	178	6.17e-21	88.2
MS.gene47008.t1	AT5G43080	37.500	152	91	4	23	171	28	178	6.34e-21	88.2
MS.gene47008.t1	AT1G80370	43.220	118	64	3	56	171	172	288	6.89e-21	89.0
MS.gene47008.t1	AT5G11300	40.000	125	72	3	49	171	140	263	2.40e-20	87.0
MS.gene47008.t1	AT5G11300	40.000	125	72	3	49	171	140	263	2.44e-20	87.0
MS.gene47008.t1	AT1G47210	44.037	109	56	3	67	171	88	195	3.90e-20	86.3
MS.gene47008.t1	AT1G47230	32.948	173	89	5	4	171	39	189	7.39e-20	85.5
MS.gene47008.t1	AT1G47210	42.453	106	56	3	67	168	88	192	1.78e-19	81.6
MS.gene47008.t1	AT5G25380	39.450	109	63	3	65	171	157	264	5.70e-18	80.5
MS.gene47008.t1	AT5G25380	39.450	109	63	3	65	171	163	270	6.61e-18	80.5
MS.gene47008.t1	AT5G25380	39.450	109	63	3	65	171	157	264	6.77e-18	80.5
MS.gene47008.t1	AT5G25380	39.450	109	63	3	65	171	157	264	6.77e-18	80.5
MS.gene47008.t1	AT1G15570	42.593	108	59	3	66	171	173	279	4.02e-17	78.2

Find 27 sgRNAs with CRISPR-Local

Find 44 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGAGTCAGATAGAAAGTTTC+TGG	0.200703	5.2:+75440930	None:intergenic
CTCCTCTTAGCCGTTATATC+TGG	0.320074	5.2:+75441005	None:intergenic
TTAGCACCTTGTGATGCATT+TGG	0.341745	5.2:+75441671	None:intergenic
AGAGAGGAATTTCAACTTGT+TGG	0.397326	5.2:-75440867	MS.gene47008:CDS
TGCTGATAAGGATAATGAAT+TGG	0.420062	5.2:-75441231	MS.gene47008:CDS
AATTACTGAACTACAGGCTT+TGG	0.426171	5.2:+75441424	None:intergenic
ACAGGCTTTGGCATTTCTCC+TGG	0.430807	5.2:+75441436	None:intergenic
AGTAATTTCAGCTAGAAGAA+AGG	0.442539	5.2:-75441408	MS.gene47008:intron
ACAAGTTGAAATTCCTCTCT+AGG	0.464310	5.2:+75440870	None:intergenic
AGCTGAAATTACTGAACTAC+AGG	0.468162	5.2:+75441418	None:intergenic
TTCTTGTGGAATTGTGCTAA+AGG	0.481895	5.2:-75441276	MS.gene47008:CDS
GAACATTGATACTGCTGATA+AGG	0.486849	5.2:-75441243	MS.gene47008:CDS
TGTCAAATTCAGGCTTCTTG+TGG	0.503060	5.2:-75441290	MS.gene47008:intron
GGCTTCACAGCCAGATATAA+CGG	0.509060	5.2:-75441015	MS.gene47008:CDS
GGAGATCAATTCTTGTTGAC+TGG	0.513087	5.2:-75440986	MS.gene47008:CDS
GGCTTTGGCATTTCTCCTGG+TGG	0.520295	5.2:+75441439	None:intergenic
TGTACGAGTACATGATTACA+TGG	0.521386	5.2:-75441036	MS.gene47008:CDS
TGACTCTTAATATTGTTGAC+CGG	0.533961	5.2:-75440914	MS.gene47008:CDS
GATCAATTCTTGTTGACTGG+TGG	0.548572	5.2:-75440983	MS.gene47008:CDS
CAAGTTGAAATTCCTCTCTA+GGG	0.572482	5.2:+75440871	None:intergenic
ATATTCTTCATCAGAGCTGA+TGG	0.577555	5.2:+75441514	None:intergenic
AGCCAGATATAACGGCTAAG+AGG	0.588033	5.2:-75441007	MS.gene47008:CDS
TGAAGAATATGCGGAAGTGA+AGG	0.590883	5.2:-75441501	MS.gene47008:CDS
CACAAAAGCTGTCCCTAGAG+AGG	0.594294	5.2:-75440883	MS.gene47008:CDS
CAGCTCTGATGAAGAATATG+CGG	0.651542	5.2:-75441510	MS.gene47008:CDS
AGTTAAGAGAAAGATCCACC+AGG	0.667171	5.2:-75441454	MS.gene47008:CDS
ATGTTTCCAAATGCATCACA+AGG	0.671375	5.2:-75441677	MS.gene47008:intron

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAAAAAAAAAAAATTAGACA+TGG	+	chr5.2:75440910-75440929	None:intergenic	10.0%
!!	ATATGAGAAATAGAAAATAA+TGG	+	chr5.2:75441131-75441150	None:intergenic	15.0%
!!!	TTTTTTTTTTTTTTTGTTGC+AGG	-	chr5.2:75440921-75440940	MS.gene47008:CDS	15.0%
!	TAGTTTCTTTCTTTCTTTGT+TGG	-	chr5.2:75441407-75441426	MS.gene47008:intron	25.0%
!	TTGAATTTCATGAATGACTT+AGG	-	chr5.2:75441444-75441463	MS.gene47008:CDS	25.0%
!!	TCTATTTCTCATATGTGATA+TGG	-	chr5.2:75441137-75441156	MS.gene47008:intron	25.0%
	AGTAATTTCAGCTAGAAGAA+AGG	-	chr5.2:75441099-75441118	MS.gene47008:intron	30.0%
	CATGGAAATAGATAAACATG+AGG	+	chr5.2:75440892-75440911	None:intergenic	30.0%
	GAACAATGTTTGTCAAATTC+AGG	-	chr5.2:75441207-75441226	MS.gene47008:CDS	30.0%
	TGACTCTTAATATTGTTGAC+CGG	-	chr5.2:75441593-75441612	MS.gene47008:intron	30.0%
	TGCTGATAAGGATAATGAAT+TGG	-	chr5.2:75441276-75441295	MS.gene47008:CDS	30.0%
!	GTGATATGGTTTTCGATTTA+GGG	-	chr5.2:75441151-75441170	MS.gene47008:intron	30.0%
!	TGTGATATGGTTTTCGATTT+AGG	-	chr5.2:75441150-75441169	MS.gene47008:intron	30.0%
!!	TAGGGTTCTATTTTTCAATG+TGG	-	chr5.2:75441169-75441188	MS.gene47008:intron	30.0%
	AATTACTGAACTACAGGCTT+TGG	+	chr5.2:75441086-75441105	None:intergenic	35.0%
	AGAGAGGAATTTCAACTTGT+TGG	-	chr5.2:75441640-75441659	MS.gene47008:intron	35.0%
	AGAGTCAGATAGAAAGTTTC+TGG	+	chr5.2:75441580-75441599	None:intergenic	35.0%
	AGCTGAAATTACTGAACTAC+AGG	+	chr5.2:75441092-75441111	None:intergenic	35.0%
	ATATTCTTCATCAGAGCTGA+TGG	+	chr5.2:75440996-75441015	None:intergenic	35.0%
	ATGTTTCCAAATGCATCACA+AGG	-	chr5.2:75440830-75440849	MS.gene47008:CDS	35.0%
	GAACATTGATACTGCTGATA+AGG	-	chr5.2:75441264-75441283	MS.gene47008:CDS	35.0%
	TGTACGAGTACATGATTACA+TGG	-	chr5.2:75441471-75441490	MS.gene47008:CDS	35.0%
	TTCTTGTGGAATTGTGCTAA+AGG	-	chr5.2:75441231-75441250	MS.gene47008:CDS	35.0%
!	ACAAGTTGAAATTCCTCTCT+AGG	+	chr5.2:75441640-75441659	None:intergenic	35.0%
!	CAAGTTGAAATTCCTCTCTA+GGG	+	chr5.2:75441639-75441658	None:intergenic	35.0%
	AGTTAAGAGAAAGATCCACC+AGG	-	chr5.2:75441053-75441072	MS.gene47008:intron	40.0%
	GGAGATCAATTCTTGTTGAC+TGG	-	chr5.2:75441521-75441540	MS.gene47008:CDS	40.0%
	TGAAGAATATGCGGAAGTGA+AGG	-	chr5.2:75441006-75441025	MS.gene47008:CDS	40.0%
	TGTCAAATTCAGGCTTCTTG+TGG	-	chr5.2:75441217-75441236	MS.gene47008:CDS	40.0%
	TTAGCACCTTGTGATGCATT+TGG	+	chr5.2:75440839-75440858	None:intergenic	40.0%
!	CAGCTCTGATGAAGAATATG+CGG	-	chr5.2:75440997-75441016	MS.gene47008:CDS	40.0%
!	GATCAATTCTTGTTGACTGG+TGG	-	chr5.2:75441524-75441543	MS.gene47008:intron	40.0%
!	GCAATAACTTCAGGTACTGT+AGG	+	chr5.2:75440974-75440993	None:intergenic	40.0%
!	TTTTGTTGCAGGACTCACTT+AGG	-	chr5.2:75440932-75440951	MS.gene47008:CDS	40.0%
!!	CGCTTTTTACAAACTCTCTG+AGG	-	chr5.2:75441327-75441346	MS.gene47008:intron	40.0%
	AGCCAGATATAACGGCTAAG+AGG	-	chr5.2:75441500-75441519	MS.gene47008:CDS	45.0%
	CTCCTCTTAGCCGTTATATC+TGG	+	chr5.2:75441505-75441524	None:intergenic	45.0%
	GAGCTGATGGCAATAACTTC+AGG	+	chr5.2:75440983-75441002	None:intergenic	45.0%
	GGCTTCACAGCCAGATATAA+CGG	-	chr5.2:75441492-75441511	MS.gene47008:CDS	45.0%
!	CAGCTTTTGTGGACAAGAAC+CGG	+	chr5.2:75441615-75441634	None:intergenic	45.0%
	CACAAAAGCTGTCCCTAGAG+AGG	-	chr5.2:75441624-75441643	MS.gene47008:intron	50.0%
!	ACAGGCTTTGGCATTTCTCC+TGG	+	chr5.2:75441074-75441093	None:intergenic	50.0%
!!	CTCTCTAGGGACAGCTTTTG+TGG	+	chr5.2:75441626-75441645	None:intergenic	50.0%
!	GGCTTTGGCATTTCTCCTGG+TGG	+	chr5.2:75441071-75441090	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.2	gene	75440824	75441705	75440824	ID=MS.gene47008
chr5.2	mRNA	75440824	75441705	75440824	ID=MS.gene47008.t1;Parent=MS.gene47008
chr5.2	exon	75441678	75441705	75441678	ID=MS.gene47008.t1.exon1;Parent=MS.gene47008.t1
chr5.2	CDS	75441678	75441705	75441678	ID=cds.MS.gene47008.t1;Parent=MS.gene47008.t1
chr5.2	exon	75441409	75441542	75441409	ID=MS.gene47008.t1.exon2;Parent=MS.gene47008.t1
chr5.2	CDS	75441409	75441542	75441409	ID=cds.MS.gene47008.t1;Parent=MS.gene47008.t1
chr5.2	exon	75441181	75441300	75441181	ID=MS.gene47008.t1.exon3;Parent=MS.gene47008.t1
chr5.2	CDS	75441181	75441300	75441181	ID=cds.MS.gene47008.t1;Parent=MS.gene47008.t1
chr5.2	exon	75440824	75441063	75440824	ID=MS.gene47008.t1.exon4;Parent=MS.gene47008.t1
chr5.2	CDS	75440824	75441063	75440824	ID=cds.MS.gene47008.t1;Parent=MS.gene47008.t1

Gene Sequence

>MS.gene47008

ATGCTAATGTTTCCAAATGCATCACAAGGTGCTAATTTTTTAATTTTATTAAAATTAAATTATTACCTCATGTTTATCTATTTCCATGTCTAATTTTTTTTTTTTTTTTTTTGTTGCAGGACTCACTTAGGTGCTATTGCTCAGAAGCCTACAGTACCTGAAGTTATTGCCATCAGCTCTGATGAAGAATATGCGGAAGTGAAGGAGAAACAAGTTGTTAAAAGAAGAAAGTTAAGAGAAAGATCCACCAGGAGAAATGCCAAAGCCTGTAGTTCAGTAATTTCAGCTAGAAGAAAGGTTTTTTCCATTATTTTCTATTTCTCATATGTGATATGGTTTTCGATTTAGGGTTCTATTTTTCAATGTGGACTAATAATAAGTATGAACAATGTTTGTCAAATTCAGGCTTCTTGTGGAATTGTGCTAAAGGATTTAGTTGTGAACATTGATACTGCTGATAAGGATAATGAATTGGCTGTAACTGAGTACGTTGATGACATCTACGCTTTTTACAAACTCTCTGAGGTAATGTTTTATGCTTTGCTTTGATTTGTTTTGTGTTCTTTCTTTATATATATGTCTCTAGTTTCTTTCTTTCTTTGTTGGCATTCTCTAATGTTTTGAATTTCATGAATGACTTAGGTTGATGTACGAGTACATGATTACATGGCTTCACAGCCAGATATAACGGCTAAGAGGAGATCAATTCTTGTTGACTGGTGGAATGACGTGCATCGAACATTCAAACTCATGCCAGAAACTTTCTATCTGACTCTTAATATTGTTGACCGGTTCTTGTCCACAAAAGCTGTCCCTAGAGAGGAATTTCAACTTGTTGGCATAAGCTCAATGCTTATAGCATCAAAATATGAAGCGAAATGA

Protein sequence

>MS.gene47008.t1

MLMFPNASQVIAISSDEEYAEVKEKQVVKRRKLRERSTRRNAKACSSVISARRKASCGIVLKDLVVNIDTADKDNELAVTEYVDDIYAFYKLSEVDVRVHDYMASQPDITAKRRSILVDWWNDVHRTFKLMPETFYLTLNIVDRFLSTKAVPREEFQLVGISSMLIASKYEAK