Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47690.t1 | XP_003617304.2 | 98.4 | 61 | 1 | 0 | 1 | 61 | 308 | 368 | 3.00E-28 | 133.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47690.t1 | G7KCY8 | 98.4 | 61 | 1 | 0 | 1 | 61 | 255 | 315 | 2.2e-28 | 133.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049669 | MS.gene47690 | 0.802859 | 4.59E-49 | -1.69E-46 |
| MS.gene049825 | MS.gene47690 | 0.812903 | 3.35E-51 | -1.69E-46 |
| MS.gene049873 | MS.gene47690 | 0.819985 | 8.71E-53 | -1.69E-46 |
| MS.gene050719 | MS.gene47690 | 0.80688 | 6.63E-50 | -1.69E-46 |
| MS.gene050721 | MS.gene47690 | 0.810125 | 1.35E-50 | -1.69E-46 |
| MS.gene052176 | MS.gene47690 | 0.82592 | 3.60E-54 | -1.69E-46 |
| MS.gene052302 | MS.gene47690 | 0.800186 | 1.62E-48 | -1.69E-46 |
| MS.gene052309 | MS.gene47690 | -0.814255 | 1.69E-51 | -1.69E-46 |
| MS.gene052358 | MS.gene47690 | -0.817653 | 2.95E-52 | -1.69E-46 |
| MS.gene052376 | MS.gene47690 | 0.802906 | 4.49E-49 | -1.69E-46 |
| MS.gene053006 | MS.gene47690 | 0.837654 | 4.57E-57 | -1.69E-46 |
| MS.gene053963 | MS.gene47690 | 0.810783 | 9.71E-51 | -1.69E-46 |
| MS.gene053995 | MS.gene47690 | 0.814568 | 1.44E-51 | -1.69E-46 |
| MS.gene055778 | MS.gene47690 | 0.809908 | 1.50E-50 | -1.69E-46 |
| MS.gene055808 | MS.gene47690 | 0.813202 | 2.88E-51 | -1.69E-46 |
| MS.gene056091 | MS.gene47690 | 0.834218 | 3.40E-56 | -1.69E-46 |
| MS.gene056506 | MS.gene47690 | 0.812755 | 3.61E-51 | -1.69E-46 |
| MS.gene057079 | MS.gene47690 | 0.843551 | 1.31E-58 | -1.69E-46 |
| MS.gene057555 | MS.gene47690 | 0.833018 | 6.79E-56 | -1.69E-46 |
| MS.gene05795 | MS.gene47690 | 0.814194 | 1.74E-51 | -1.69E-46 |
| MS.gene058037 | MS.gene47690 | 0.809007 | 2.34E-50 | -1.69E-46 |
| MS.gene058635 | MS.gene47690 | 0.801434 | 9.02E-49 | -1.69E-46 |
| MS.gene05953 | MS.gene47690 | 0.809516 | 1.82E-50 | -1.69E-46 |
| MS.gene05954 | MS.gene47690 | 0.84836 | 6.44E-60 | -1.69E-46 |
| MS.gene061040 | MS.gene47690 | -0.849446 | 3.22E-60 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47690.t1 | MTR_5g090130 | 98.361 | 61 | 1 | 0 | 1 | 61 | 255 | 315 | 1.07e-39 | 132 |
| MS.gene47690.t1 | MTR_5g090130 | 98.361 | 61 | 1 | 0 | 1 | 61 | 307 | 367 | 1.70e-39 | 132 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47690.t1 | AT1G30540 | 72.000 | 50 | 14 | 0 | 1 | 50 | 229 | 278 | 2.43e-18 | 75.9 |
| MS.gene47690.t1 | AT1G30540 | 72.000 | 50 | 14 | 0 | 1 | 50 | 229 | 278 | 2.43e-18 | 75.9 |
| MS.gene47690.t1 | AT1G30540 | 72.000 | 50 | 14 | 0 | 1 | 50 | 300 | 349 | 3.24e-18 | 75.9 |
Find 18 sgRNAs with CRISPR-Local
Find 25 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGCACCCCAGGAAAGTATT+TGG | 0.301399 | 5.3:+72705571 | None:intergenic |
| TGCATTTCCAAATACTTTCC+TGG | 0.311592 | 5.3:-72705578 | MS.gene47690:CDS |
| TATACTGACCTTAGGTCTAA+TGG | 0.311823 | 5.3:+72705547 | None:intergenic |
| ATACTGACCTTAGGTCTAAT+GGG | 0.413875 | 5.3:+72705548 | None:intergenic |
| GCATTTCCAAATACTTTCCT+GGG | 0.443620 | 5.3:-72705577 | MS.gene47690:CDS |
| GCAAACACAAAGTGGGATGT+AGG | 0.461172 | 5.3:-72705617 | MS.gene47690:CDS |
| TTGGAGCAGCATGGTTAGCT+TGG | 0.461737 | 5.3:-72705350 | MS.gene47690:CDS |
| GGTCTAATGGGAAGCACCCC+AGG | 0.482189 | 5.3:+72705560 | None:intergenic |
| TTTCCGCTTGTCATGGTTGG+TGG | 0.487092 | 5.3:-72705650 | None:intergenic |
| AACCATGCTGCTCCAACAGC+AGG | 0.514568 | 5.3:+72705357 | None:intergenic |
| CAAACACAAAGTGGGATGTA+GGG | 0.530904 | 5.3:-72705616 | MS.gene47690:CDS |
| GGTGCTTCCCATTAGACCTA+AGG | 0.571799 | 5.3:-72705555 | MS.gene47690:intron |
| TTCTTGAAGCAAACACAAAG+TGG | 0.592334 | 5.3:-72705625 | MS.gene47690:CDS |
| TCTTGAAGCAAACACAAAGT+GGG | 0.629952 | 5.3:-72705624 | MS.gene47690:CDS |
| AAACAGGTAGAGCCTGCTGT+TGG | 0.632953 | 5.3:-72705369 | MS.gene47690:intron |
| CATTTCCAAATACTTTCCTG+GGG | 0.634649 | 5.3:-72705576 | MS.gene47690:CDS |
| TTTCATGAAAGAATATAACA+AGG | 0.640802 | 5.3:-72705322 | MS.gene47690:CDS |
| AGCCTGCTGTTGGAGCAGCA+TGG | 0.659631 | 5.3:-72705359 | MS.gene47690:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATAGAATGAAATTTTAAATT+TGG | - | chr5.3:72705543-72705562 | MS.gene47690:intron | 10.0% |
| !! | TTTCATGAAAGAATATAACA+AGG | - | chr5.3:72705621-72705640 | MS.gene47690:CDS | 20.0% |
| !!! | TCGTCATTTATTTTATTTGT+TGG | - | chr5.3:72705421-72705440 | MS.gene47690:intron | 20.0% |
| !! | AAATTTGGTTTATTGCAAAC+AGG | - | chr5.3:72705558-72705577 | MS.gene47690:CDS | 25.0% |
| TCATTCTATGCTAAAGATTC+TGG | + | chr5.3:72705532-72705551 | None:intergenic | 30.0% | |
| ATACTGACCTTAGGTCTAAT+GGG | + | chr5.3:72705398-72705417 | None:intergenic | 35.0% | |
| ATTTACTAAGCCTCTAAGCA+AGG | + | chr5.3:72705501-72705520 | None:intergenic | 35.0% | |
| CATTTCCAAATACTTTCCTG+GGG | - | chr5.3:72705367-72705386 | MS.gene47690:intron | 35.0% | |
| GCATTTCCAAATACTTTCCT+GGG | - | chr5.3:72705366-72705385 | MS.gene47690:CDS | 35.0% | |
| TATACTGACCTTAGGTCTAA+TGG | + | chr5.3:72705399-72705418 | None:intergenic | 35.0% | |
| TCTTGAAGCAAACACAAAGT+GGG | - | chr5.3:72705319-72705338 | MS.gene47690:CDS | 35.0% | |
| TGACGATATATACTGACCTT+AGG | + | chr5.3:72705407-72705426 | None:intergenic | 35.0% | |
| TGCATTTCCAAATACTTTCC+TGG | - | chr5.3:72705365-72705384 | MS.gene47690:CDS | 35.0% | |
| TTCTTGAAGCAAACACAAAG+TGG | - | chr5.3:72705318-72705337 | MS.gene47690:CDS | 35.0% | |
| CAAACACAAAGTGGGATGTA+GGG | - | chr5.3:72705327-72705346 | MS.gene47690:CDS | 40.0% | |
| GCTTTCTACGTCTTTATTGC+AGG | - | chr5.3:72705443-72705462 | MS.gene47690:intron | 40.0% | |
| GATGCACTAACCTTGCTTAG+AGG | - | chr5.3:72705488-72705507 | MS.gene47690:intron | 45.0% | |
| GCAAACACAAAGTGGGATGT+AGG | - | chr5.3:72705326-72705345 | MS.gene47690:CDS | 45.0% | |
| !! | AAGCACCCCAGGAAAGTATT+TGG | + | chr5.3:72705375-72705394 | None:intergenic | 45.0% |
| AAACAGGTAGAGCCTGCTGT+TGG | - | chr5.3:72705574-72705593 | MS.gene47690:CDS | 50.0% | |
| GGTGCTTCCCATTAGACCTA+AGG | - | chr5.3:72705388-72705407 | MS.gene47690:intron | 50.0% | |
| ! | TTGGAGCAGCATGGTTAGCT+TGG | - | chr5.3:72705593-72705612 | MS.gene47690:CDS | 50.0% |
| AACCATGCTGCTCCAACAGC+AGG | + | chr5.3:72705589-72705608 | None:intergenic | 55.0% | |
| AGCCTGCTGTTGGAGCAGCA+TGG | - | chr5.3:72705584-72705603 | MS.gene47690:CDS | 60.0% | |
| !! | GGTCTAATGGGAAGCACCCC+AGG | + | chr5.3:72705386-72705405 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 72705305 | 72705660 | 72705305 | ID=MS.gene47690 |
| chr5.3 | mRNA | 72705305 | 72705660 | 72705305 | ID=MS.gene47690.t1;Parent=MS.gene47690 |
| chr5.3 | exon | 72705556 | 72705660 | 72705556 | ID=MS.gene47690.t1.exon1;Parent=MS.gene47690.t1 |
| chr5.3 | CDS | 72705556 | 72705660 | 72705556 | ID=cds.MS.gene47690.t1;Parent=MS.gene47690.t1 |
| chr5.3 | exon | 72705305 | 72705385 | 72705305 | ID=MS.gene47690.t1.exon2;Parent=MS.gene47690.t1 |
| chr5.3 | CDS | 72705305 | 72705385 | 72705305 | ID=cds.MS.gene47690.t1;Parent=MS.gene47690.t1 |
| Gene Sequence |
| Protein sequence |
