Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50528.t1 | XP_013449674.1 | 85 | 227 | 31 | 3 | 1 | 226 | 177 | 401 | 1.60E-87 | 332.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50528.t1 | A0A072U1Y0 | 85.0 | 227 | 31 | 3 | 1 | 226 | 177 | 401 | 1.2e-87 | 332.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049230 | MS.gene50528 | 0.815252 | 1.02E-51 | -1.69E-46 |
| MS.gene049293 | MS.gene50528 | -0.83919 | 1.84E-57 | -1.69E-46 |
| MS.gene049653 | MS.gene50528 | 0.802551 | 5.32E-49 | -1.69E-46 |
| MS.gene050623 | MS.gene50528 | -0.800411 | 1.46E-48 | -1.69E-46 |
| MS.gene052309 | MS.gene50528 | -0.815937 | 7.16E-52 | -1.69E-46 |
| MS.gene052954 | MS.gene50528 | 0.820514 | 6.59E-53 | -1.69E-46 |
| MS.gene053963 | MS.gene50528 | 0.818799 | 1.62E-52 | -1.69E-46 |
| MS.gene054946 | MS.gene50528 | -0.818047 | 2.40E-52 | -1.69E-46 |
| MS.gene057079 | MS.gene50528 | 0.841233 | 5.38E-58 | -1.69E-46 |
| MS.gene057999 | MS.gene50528 | 0.831419 | 1.69E-55 | -1.69E-46 |
| MS.gene058074 | MS.gene50528 | 0.801391 | 9.21E-49 | -1.69E-46 |
| MS.gene058238 | MS.gene50528 | 0.841122 | 5.75E-58 | -1.69E-46 |
| MS.gene059872 | MS.gene50528 | -0.801074 | 1.07E-48 | -1.69E-46 |
| MS.gene060297 | MS.gene50528 | 0.81431 | 1.64E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50528.t1 | MTR_7g094240 | 87.665 | 227 | 25 | 3 | 1 | 226 | 177 | 401 | 6.68e-107 | 313 |
| MS.gene50528.t1 | MTR_1g089850 | 46.875 | 288 | 88 | 10 | 1 | 226 | 175 | 459 | 1.08e-44 | 155 |
| MS.gene50528.t1 | MTR_2g021070 | 37.349 | 249 | 125 | 8 | 1 | 220 | 206 | 452 | 4.81e-28 | 110 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50528.t1 | AT1G03350 | 37.218 | 266 | 113 | 9 | 1 | 214 | 192 | 455 | 7.44e-32 | 121 |
| MS.gene50528.t1 | AT4G13110 | 56.061 | 66 | 28 | 1 | 4 | 68 | 174 | 239 | 6.84e-16 | 75.5 |
Find 59 sgRNAs with CRISPR-Local
Find 65 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTGGAAGAGGTGGATGATTT+TGG | 0.211405 | 7.3:-16666413 | MS.gene50528:CDS |
| GTGAAAAGAACAATAGTTTA+TGG | 0.221360 | 7.3:-16666373 | MS.gene50528:CDS |
| ATATTATTATAAGGTTTATA+AGG | 0.296101 | 7.3:-16666567 | MS.gene50528:CDS |
| TGGTTCCTAATGTTGTTGAT+AGG | 0.315634 | 7.3:-16666607 | MS.gene50528:CDS |
| ATCAAAAGTTGATGAGCATT+TGG | 0.347807 | 7.3:-16666330 | MS.gene50528:CDS |
| TTGGTTGAGATATTATTATA+AGG | 0.389321 | 7.3:-16666576 | MS.gene50528:CDS |
| GTTTCCCTATCAACAACATT+AGG | 0.391217 | 7.3:+16666602 | None:intergenic |
| GAAAAGAAGGCAATGAGATT+GGG | 0.398844 | 7.3:-16666116 | MS.gene50528:CDS |
| AAGAAGAAGAAGATTTGAGT+TGG | 0.406673 | 7.3:-16666040 | MS.gene50528:CDS |
| GGAAAAGAAGGCAATGAGAT+TGG | 0.407669 | 7.3:-16666117 | MS.gene50528:CDS |
| AGAAGAAGAAGATTTGAGTT+GGG | 0.413686 | 7.3:-16666039 | MS.gene50528:CDS |
| GTTAAGAGAGAATGATGAAA+TGG | 0.416221 | 7.3:-16666648 | MS.gene50528:CDS |
| AAATGGAGAGTGTATTTAGA+AGG | 0.417338 | 7.3:-16666631 | MS.gene50528:CDS |
| GCAAAATTGATTTGCGGAAG+CGG | 0.446892 | 7.3:-16666079 | MS.gene50528:CDS |
| GGAGAGTGTATTTAGAAGGT+TGG | 0.446991 | 7.3:-16666627 | MS.gene50528:CDS |
| AGAGGATGAGGACTTGAGTT+GGG | 0.453903 | 7.3:-16666492 | MS.gene50528:CDS |
| GAAACGAATGTCGAGGGAAG+AGG | 0.455231 | 7.3:-16666510 | MS.gene50528:CDS |
| AAGAGGATGAGGACTTGAGT+TGG | 0.459371 | 7.3:-16666493 | MS.gene50528:CDS |
| GAGATTGAGTAATGTGGAAG+AGG | 0.460129 | 7.3:-16666426 | MS.gene50528:CDS |
| GGTTCCTAATGTTGTTGATA+GGG | 0.469145 | 7.3:-16666606 | MS.gene50528:CDS |
| AATAAATCTCAAGCCTTGGC+AGG | 0.469300 | 7.3:+16665993 | None:intergenic |
| TGACAATGGAGAAGAATGTC+GGG | 0.483635 | 7.3:-16666232 | MS.gene50528:CDS |
| TTTATCCACTTGGCTTCCAT+CGG | 0.511757 | 7.3:+16666289 | None:intergenic |
| AATGTCGAGGGAAGAGGATG+AGG | 0.511956 | 7.3:-16666504 | MS.gene50528:CDS |
| GCTTGAGTGGGATGAGATTG+AGG | 0.513354 | 7.3:-16666156 | MS.gene50528:CDS |
| AAAAGAAGGCAATGAGATTG+GGG | 0.517259 | 7.3:-16666115 | MS.gene50528:CDS |
| AATCCTTCGCTTTATCCACT+TGG | 0.517564 | 7.3:+16666279 | None:intergenic |
| TAGTAGCAATGAGGAAAAGA+AGG | 0.522851 | 7.3:-16666129 | MS.gene50528:CDS |
| GCCCAAGCAAAATTGATTTG+CGG | 0.523784 | 7.3:-16666085 | MS.gene50528:CDS |
| GGTAAGTCAGAACTCGGAGC+AGG | 0.524997 | 7.3:-16666192 | MS.gene50528:CDS |
| TTTATGGCAAAACAAGATGA+TGG | 0.527238 | 7.3:-16666357 | MS.gene50528:CDS |
| GTATAATAAATCTCAAGCCT+TGG | 0.537750 | 7.3:+16665989 | None:intergenic |
| TTTGGATGTTGTTCAAGAGA+AGG | 0.539257 | 7.3:-16666312 | MS.gene50528:CDS |
| ATTGAGTAATGTGGAAGAGG+TGG | 0.540140 | 7.3:-16666423 | MS.gene50528:CDS |
| ATGGAGAAGAATGTCGGGGA+TGG | 0.549119 | 7.3:-16666227 | MS.gene50528:CDS |
| AAGTCAGAACTCGGAGCAGG+AGG | 0.552496 | 7.3:-16666189 | MS.gene50528:CDS |
| GTGACAATGGAGAAGAATGT+CGG | 0.570801 | 7.3:-16666233 | MS.gene50528:CDS |
| TTCAGAGGTAAGTCAGAACT+CGG | 0.575765 | 7.3:-16666198 | MS.gene50528:CDS |
| GTCAGAACTCGGAGCAGGAG+GGG | 0.584283 | 7.3:-16666187 | MS.gene50528:CDS |
| AAGGCAATGAGATTGGGGAG+TGG | 0.594208 | 7.3:-16666110 | MS.gene50528:CDS |
| TTAAGAAAGCTGAGGATGTG+AGG | 0.599039 | 7.3:-16666544 | MS.gene50528:CDS |
| GATGAGGAGTATGAAGATGA+TGG | 0.606982 | 7.3:-16666461 | MS.gene50528:CDS |
| TTCTGATAAGAAAGTGACAA+TGG | 0.614400 | 7.3:-16666246 | MS.gene50528:CDS |
| AGTCAGAACTCGGAGCAGGA+GGG | 0.616491 | 7.3:-16666188 | MS.gene50528:CDS |
| TAAGAAAGCTGAGGATGTGA+GGG | 0.620596 | 7.3:-16666543 | MS.gene50528:CDS |
| GTTGTTCAAGAGAAGGCCGA+TGG | 0.623363 | 7.3:-16666305 | MS.gene50528:CDS |
| AGCTCGTGAAACGAATGTCG+AGG | 0.623979 | 7.3:-16666517 | MS.gene50528:CDS |
| TTATAAGGTTAAGAAAGCTG+AGG | 0.632810 | 7.3:-16666552 | MS.gene50528:CDS |
| AAGCCAAGTGGATAAAGCGA+AGG | 0.635396 | 7.3:-16666282 | MS.gene50528:CDS |
| GAGTTGGGATGTTGAAGATG+AGG | 0.640727 | 7.3:-16666477 | MS.gene50528:CDS |
| TGATAAGAGATTGAGTAATG+TGG | 0.641001 | 7.3:-16666432 | MS.gene50528:CDS |
| GGATAAAGCGAAGGATTCTG+TGG | 0.643867 | 7.3:-16666273 | MS.gene50528:CDS |
| GCTCGTGAAACGAATGTCGA+GGG | 0.648458 | 7.3:-16666516 | MS.gene50528:CDS |
| TGATGATGATGAGCCTGCCA+AGG | 0.653151 | 7.3:-16666006 | MS.gene50528:CDS |
| CGGGGATGGAAAGTCTTCAG+AGG | 0.655419 | 7.3:-16666213 | MS.gene50528:CDS |
| GAAGGCCGATGGAAGCCAAG+TGG | 0.667743 | 7.3:-16666294 | MS.gene50528:CDS |
| ATGGCAAAACAAGATGATGG+AGG | 0.669587 | 7.3:-16666354 | MS.gene50528:CDS |
| TGAGGATCTTAGTAGCAATG+AGG | 0.687708 | 7.3:-16666138 | MS.gene50528:CDS |
| GACAATGGAGAAGAATGTCG+GGG | 0.696218 | 7.3:-16666231 | MS.gene50528:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATATTATTATAAGGTTTATA+AGG | - | chr7.3:16666093-16666112 | MS.gene50528:CDS | 10.0% |
| !! | TTGGTTGAGATATTATTATA+AGG | - | chr7.3:16666084-16666103 | MS.gene50528:CDS | 20.0% |
| ! | GTGAAAAGAACAATAGTTTA+TGG | - | chr7.3:16666287-16666306 | MS.gene50528:CDS | 25.0% |
| AAGAAGAAGAAGATTTGAGT+TGG | - | chr7.3:16666620-16666639 | MS.gene50528:CDS | 30.0% | |
| GTTAAGAGAGAATGATGAAA+TGG | - | chr7.3:16666012-16666031 | MS.gene50528:CDS | 30.0% | |
| TGATAAGAGATTGAGTAATG+TGG | - | chr7.3:16666228-16666247 | MS.gene50528:CDS | 30.0% | |
| TTATAAGGTTAAGAAAGCTG+AGG | - | chr7.3:16666108-16666127 | MS.gene50528:CDS | 30.0% | |
| TTCTGATAAGAAAGTGACAA+TGG | - | chr7.3:16666414-16666433 | MS.gene50528:CDS | 30.0% | |
| TTTATGGCAAAACAAGATGA+TGG | - | chr7.3:16666303-16666322 | MS.gene50528:CDS | 30.0% | |
| ! | AAATGGAGAGTGTATTTAGA+AGG | - | chr7.3:16666029-16666048 | MS.gene50528:CDS | 30.0% |
| ! | AGAAGAAGAAGATTTGAGTT+GGG | - | chr7.3:16666621-16666640 | MS.gene50528:CDS | 30.0% |
| !! | ATCAAAAGTTGATGAGCATT+TGG | - | chr7.3:16666330-16666349 | MS.gene50528:CDS | 30.0% |
| !! | TTGTTGATAGGGAAACTTTT+TGG | - | chr7.3:16666065-16666084 | MS.gene50528:CDS | 30.0% |
| AAAAGAAGGCAATGAGATTG+GGG | - | chr7.3:16666545-16666564 | MS.gene50528:CDS | 35.0% | |
| GAAAAGAAGGCAATGAGATT+GGG | - | chr7.3:16666544-16666563 | MS.gene50528:CDS | 35.0% | |
| GGTTCCTAATGTTGTTGATA+GGG | - | chr7.3:16666054-16666073 | MS.gene50528:CDS | 35.0% | |
| GTTTCCCTATCAACAACATT+AGG | + | chr7.3:16666061-16666080 | None:intergenic | 35.0% | |
| TAGTAGCAATGAGGAAAAGA+AGG | - | chr7.3:16666531-16666550 | MS.gene50528:CDS | 35.0% | |
| TGGTTCCTAATGTTGTTGAT+AGG | - | chr7.3:16666053-16666072 | MS.gene50528:CDS | 35.0% | |
| TTTGGATGTTGTTCAAGAGA+AGG | - | chr7.3:16666348-16666367 | MS.gene50528:CDS | 35.0% | |
| ! | TCCGCAAATCAATTTTGCTT+GGG | + | chr7.3:16666579-16666598 | None:intergenic | 35.0% |
| ! | TTCCGCAAATCAATTTTGCT+TGG | + | chr7.3:16666580-16666599 | None:intergenic | 35.0% |
| AATCCTTCGCTTTATCCACT+TGG | + | chr7.3:16666384-16666403 | None:intergenic | 40.0% | |
| ATTGAGTAATGTGGAAGAGG+TGG | - | chr7.3:16666237-16666256 | MS.gene50528:CDS | 40.0% | |
| GAGATTGAGTAATGTGGAAG+AGG | - | chr7.3:16666234-16666253 | MS.gene50528:CDS | 40.0% | |
| GGAAAAGAAGGCAATGAGAT+TGG | - | chr7.3:16666543-16666562 | MS.gene50528:CDS | 40.0% | |
| GTGACAATGGAGAAGAATGT+CGG | - | chr7.3:16666427-16666446 | MS.gene50528:CDS | 40.0% | |
| TAAGAAAGCTGAGGATGTGA+GGG | - | chr7.3:16666117-16666136 | MS.gene50528:CDS | 40.0% | |
| TGACAATGGAGAAGAATGTC+GGG | - | chr7.3:16666428-16666447 | MS.gene50528:CDS | 40.0% | |
| TGAGGATCTTAGTAGCAATG+AGG | - | chr7.3:16666522-16666541 | MS.gene50528:CDS | 40.0% | |
| TTAAGAAAGCTGAGGATGTG+AGG | - | chr7.3:16666116-16666135 | MS.gene50528:CDS | 40.0% | |
| TTCAGAGGTAAGTCAGAACT+CGG | - | chr7.3:16666462-16666481 | MS.gene50528:CDS | 40.0% | |
| TTTATCCACTTGGCTTCCAT+CGG | + | chr7.3:16666374-16666393 | None:intergenic | 40.0% | |
| ! | AAGAGGTGGATGATTTTGGA+GGG | - | chr7.3:16666251-16666270 | MS.gene50528:CDS | 40.0% |
| ! | ATGGCAAAACAAGATGATGG+AGG | - | chr7.3:16666306-16666325 | MS.gene50528:CDS | 40.0% |
| ! | GATGAGGAGTATGAAGATGA+TGG | - | chr7.3:16666199-16666218 | MS.gene50528:CDS | 40.0% |
| ! | GGAGAGTGTATTTAGAAGGT+TGG | - | chr7.3:16666033-16666052 | MS.gene50528:CDS | 40.0% |
| !! | GCAAAATTGATTTGCGGAAG+CGG | - | chr7.3:16666581-16666600 | MS.gene50528:CDS | 40.0% |
| !! | GCCCAAGCAAAATTGATTTG+CGG | - | chr7.3:16666575-16666594 | MS.gene50528:CDS | 40.0% |
| AAGAGGATGAGGACTTGAGT+TGG | - | chr7.3:16666167-16666186 | MS.gene50528:CDS | 45.0% | |
| AAGCCAAGTGGATAAAGCGA+AGG | - | chr7.3:16666378-16666397 | MS.gene50528:CDS | 45.0% | |
| AGAGGATGAGGACTTGAGTT+GGG | - | chr7.3:16666168-16666187 | MS.gene50528:CDS | 45.0% | |
| AGGGGAAAAAAGAGCTTGAG+TGG | - | chr7.3:16666491-16666510 | MS.gene50528:CDS | 45.0% | |
| GACAATGGAGAAGAATGTCG+GGG | - | chr7.3:16666429-16666448 | MS.gene50528:CDS | 45.0% | |
| GAGTTGGGATGTTGAAGATG+AGG | - | chr7.3:16666183-16666202 | MS.gene50528:CDS | 45.0% | |
| GGATAAAGCGAAGGATTCTG+TGG | - | chr7.3:16666387-16666406 | MS.gene50528:CDS | 45.0% | |
| GGGGAAAAAAGAGCTTGAGT+GGG | - | chr7.3:16666492-16666511 | MS.gene50528:CDS | 45.0% | |
| ! | GAAGAGGTGGATGATTTTGG+AGG | - | chr7.3:16666250-16666269 | MS.gene50528:CDS | 45.0% |
| ! | GTGGAAGAGGTGGATGATTT+TGG | - | chr7.3:16666247-16666266 | MS.gene50528:CDS | 45.0% |
| !! | AGAGGTGGATGATTTTGGAG+GGG | - | chr7.3:16666252-16666271 | MS.gene50528:CDS | 45.0% |
| AAGGCAATGAGATTGGGGAG+TGG | - | chr7.3:16666550-16666569 | MS.gene50528:CDS | 50.0% | |
| AATGTCGAGGGAAGAGGATG+AGG | - | chr7.3:16666156-16666175 | MS.gene50528:CDS | 50.0% | |
| AGCTCGTGAAACGAATGTCG+AGG | - | chr7.3:16666143-16666162 | MS.gene50528:CDS | 50.0% | |
| ATGGAGAAGAATGTCGGGGA+TGG | - | chr7.3:16666433-16666452 | MS.gene50528:CDS | 50.0% | |
| GAAACGAATGTCGAGGGAAG+AGG | - | chr7.3:16666150-16666169 | MS.gene50528:CDS | 50.0% | |
| GCTCGTGAAACGAATGTCGA+GGG | - | chr7.3:16666144-16666163 | MS.gene50528:CDS | 50.0% | |
| GCTTGAGTGGGATGAGATTG+AGG | - | chr7.3:16666504-16666523 | MS.gene50528:CDS | 50.0% | |
| GTTGTTCAAGAGAAGGCCGA+TGG | - | chr7.3:16666355-16666374 | MS.gene50528:CDS | 50.0% | |
| ! | TGATGATGATGAGCCTGCCA+AGG | - | chr7.3:16666654-16666673 | MS.gene50528:CDS | 50.0% |
| AAGTCAGAACTCGGAGCAGG+AGG | - | chr7.3:16666471-16666490 | MS.gene50528:CDS | 55.0% | |
| AGTCAGAACTCGGAGCAGGA+GGG | - | chr7.3:16666472-16666491 | MS.gene50528:CDS | 55.0% | |
| CGGGGATGGAAAGTCTTCAG+AGG | - | chr7.3:16666447-16666466 | MS.gene50528:CDS | 55.0% | |
| GGTAAGTCAGAACTCGGAGC+AGG | - | chr7.3:16666468-16666487 | MS.gene50528:CDS | 55.0% | |
| GAAGGCCGATGGAAGCCAAG+TGG | - | chr7.3:16666366-16666385 | MS.gene50528:CDS | 60.0% | |
| GTCAGAACTCGGAGCAGGAG+GGG | - | chr7.3:16666473-16666492 | MS.gene50528:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 16666001 | 16666681 | 16666001 | ID=MS.gene50528 |
| chr7.3 | mRNA | 16666001 | 16666681 | 16666001 | ID=MS.gene50528.t1;Parent=MS.gene50528 |
| chr7.3 | exon | 16666001 | 16666681 | 16666001 | ID=MS.gene50528.t1.exon1;Parent=MS.gene50528.t1 |
| chr7.3 | CDS | 16666001 | 16666681 | 16666001 | ID=cds.MS.gene50528.t1;Parent=MS.gene50528.t1 |
| Gene Sequence |
| Protein sequence |
