Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51069.t1 | AES89192.2 | 94.8 | 210 | 11 | 0 | 1 | 210 | 90 | 299 | 1.50E-103 | 385.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51069.t1 | G7JIL0 | 94.8 | 210 | 11 | 0 | 1 | 210 | 90 | 299 | 1.1e-103 | 385.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049378 | MS.gene51069 | 0.804789 | 1.83E-49 | -1.69E-46 |
| MS.gene050227 | MS.gene51069 | 0.814924 | 1.20E-51 | -1.69E-46 |
| MS.gene050228 | MS.gene51069 | 0.813002 | 3.19E-51 | -1.69E-46 |
| MS.gene050303 | MS.gene51069 | 0.825249 | 5.19E-54 | -1.69E-46 |
| MS.gene050330 | MS.gene51069 | -0.807445 | 5.04E-50 | -1.69E-46 |
| MS.gene050623 | MS.gene51069 | -0.801269 | 9.75E-49 | -1.69E-46 |
| MS.gene050642 | MS.gene51069 | 0.815521 | 8.86E-52 | -1.69E-46 |
| MS.gene050953 | MS.gene51069 | 0.804106 | 2.53E-49 | -1.69E-46 |
| MS.gene051349 | MS.gene51069 | 0.802864 | 4.58E-49 | -1.69E-46 |
| MS.gene051983 | MS.gene51069 | 0.805996 | 1.02E-49 | -1.69E-46 |
| MS.gene052176 | MS.gene51069 | 0.806241 | 9.05E-50 | -1.69E-46 |
| MS.gene052286 | MS.gene51069 | 0.823161 | 1.61E-53 | -1.69E-46 |
| MS.gene052302 | MS.gene51069 | 0.846365 | 2.27E-59 | -1.69E-46 |
| MS.gene052309 | MS.gene51069 | -0.818918 | 1.53E-52 | -1.69E-46 |
| MS.gene052376 | MS.gene51069 | 0.860046 | 2.71E-63 | -1.69E-46 |
| MS.gene054930 | MS.gene51069 | 0.809966 | 1.46E-50 | -1.69E-46 |
| MS.gene054942 | MS.gene51069 | 0.809503 | 1.83E-50 | -1.69E-46 |
| MS.gene056631 | MS.gene51069 | 0.807094 | 5.98E-50 | -1.69E-46 |
| MS.gene056979 | MS.gene51069 | 0.820204 | 7.76E-53 | -1.69E-46 |
| MS.gene057079 | MS.gene51069 | 0.813751 | 2.18E-51 | -1.69E-46 |
| MS.gene05795 | MS.gene51069 | 0.814439 | 1.54E-51 | -1.69E-46 |
| MS.gene059249 | MS.gene51069 | 0.806224 | 9.13E-50 | -1.69E-46 |
| MS.gene05954 | MS.gene51069 | 0.811073 | 8.40E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 43 sgRNAs with CRISPR-Local
Find 71 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTGTGGTGATGATTCTTTC+TGG | 0.187016 | 4.1:+31960250 | MS.gene51069:CDS |
| TGATGACAAGGTGTCAGATT+TGG | 0.326241 | 4.1:+31960281 | MS.gene51069:CDS |
| GCACAGGAGGGATTCAATAT+TGG | 0.328694 | 4.1:+31960491 | MS.gene51069:CDS |
| CAAGGTATCACCGTCTATTT+CGG | 0.331870 | 4.1:-31960988 | None:intergenic |
| TGTCGACAACAGATGCTCTT+AGG | 0.333659 | 4.1:+31960785 | MS.gene51069:CDS |
| CAGTCCGTGCTGGTTATAGC+TGG | 0.342284 | 4.1:+31960525 | MS.gene51069:CDS |
| ATTAGAATTAAGTTCAAATC+AGG | 0.362176 | 4.1:+31959825 | MS.gene51069:CDS |
| ATCACCGTCTATTTCGGTTC+AGG | 0.380910 | 4.1:-31960982 | None:intergenic |
| TTGTGGTGATGATTCTTTCT+GGG | 0.417652 | 4.1:+31960251 | MS.gene51069:CDS |
| GGTAAATGACTGTCAATTGC+AGG | 0.425602 | 4.1:-31960961 | None:intergenic |
| GAAAAGAGAAATGAATTTCA+AGG | 0.431576 | 4.1:+31960739 | MS.gene51069:CDS |
| AGATCAATTAAAAGAGAAGT+TGG | 0.449301 | 4.1:+31960705 | MS.gene51069:CDS |
| GTCAAAATACCGTATGGAAC+TGG | 0.462806 | 4.1:-31960329 | None:intergenic |
| GATCACGGTGATAAGTTAAA+CGG | 0.487894 | 4.1:+31959853 | MS.gene51069:CDS |
| ACAAGGTGTCAGATTTGGAC+AGG | 0.492614 | 4.1:+31960286 | MS.gene51069:CDS |
| AGTCCGTGCTGGTTATAGCT+GGG | 0.498770 | 4.1:+31960526 | MS.gene51069:CDS |
| AGAGAAGTTGGTTGAAACAC+TGG | 0.511159 | 4.1:+31960717 | MS.gene51069:CDS |
| GTGTTGTAAGAGGTGCATCC+AGG | 0.517186 | 4.1:+31960549 | MS.gene51069:CDS |
| TGTCAGATTTGGACAGGGAG+TGG | 0.520323 | 4.1:+31960292 | MS.gene51069:CDS |
| AGAAGGTCTTATAGCAGCAA+AGG | 0.527678 | 4.1:+31960460 | MS.gene51069:CDS |
| TTTAAACAATCAGTCCGTGC+TGG | 0.530482 | 4.1:+31960515 | MS.gene51069:CDS |
| ATTGTATTGCAGAATTCAGA+TGG | 0.539705 | 4.1:+31959753 | None:intergenic |
| CTTTGAAACAAAATGAGCAC+GGG | 0.542756 | 4.1:+31960839 | MS.gene51069:CDS |
| ATTTCAAGGGCTGTATGAAT+CGG | 0.544130 | 4.1:+31960753 | MS.gene51069:CDS |
| ATTTGGACAGGGAGTGGCAA+AGG | 0.545598 | 4.1:+31960298 | MS.gene51069:CDS |
| CTGGGATGATTCTGATGACA+AGG | 0.547973 | 4.1:+31960269 | MS.gene51069:CDS |
| AAAGGAAGCTTCTGCACAGG+AGG | 0.553231 | 4.1:+31960478 | MS.gene51069:CDS |
| CTCCTTTGACAAATTATCGT+GGG | 0.559200 | 4.1:+31960911 | MS.gene51069:CDS |
| TATAGCTGGGGTGTTGTAAG+AGG | 0.565319 | 4.1:+31960539 | MS.gene51069:CDS |
| AGCAAAGGAAGCTTCTGCAC+AGG | 0.565604 | 4.1:+31960475 | MS.gene51069:CDS |
| TGCCCACGATAATTTGTCAA+AGG | 0.576502 | 4.1:-31960913 | None:intergenic |
| AAAAGAGAAATGAATTTCAA+GGG | 0.589649 | 4.1:+31960740 | MS.gene51069:CDS |
| GTCCGTGCTGGTTATAGCTG+GGG | 0.598944 | 4.1:+31960527 | MS.gene51069:CDS |
| GCTTTGAAACAAAATGAGCA+CGG | 0.601754 | 4.1:+31960838 | MS.gene51069:CDS |
| TCAAATCAGGCTGATGATCA+CGG | 0.609515 | 4.1:+31959838 | MS.gene51069:CDS |
| CTTTAGAGTGGGTATCGAGA+AGG | 0.610472 | 4.1:+31960443 | MS.gene51069:intron |
| TCTCCTTTGACAAATTATCG+TGG | 0.612891 | 4.1:+31960910 | MS.gene51069:CDS |
| GAGGCGTGACCAGTTCCATA+CGG | 0.618257 | 4.1:+31960320 | MS.gene51069:CDS |
| TGGACAGGGAGTGGCAAAGG+AGG | 0.620551 | 4.1:+31960301 | MS.gene51069:CDS |
| AAGGAAGCTTCTGCACAGGA+GGG | 0.643430 | 4.1:+31960479 | MS.gene51069:CDS |
| CAAGGTGTCAGATTTGGACA+GGG | 0.648602 | 4.1:+31960287 | MS.gene51069:CDS |
| CACCCCAGCTATAACCAGCA+CGG | 0.660587 | 4.1:-31960529 | None:intergenic |
| TTTGAAACAAAATGAGCACG+GGG | 0.723764 | 4.1:+31960840 | MS.gene51069:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAATTCATAACTAAAATA+AGG | - | chr4.1:31960379-31960398 | None:intergenic | 10.0% |
| !!! | TTATTTTCTTTTTCATTTTT+TGG | + | chr4.1:31960653-31960672 | MS.gene51069:intron | 10.0% |
| !! | TATGTATAAACTATTGAAAT+TGG | + | chr4.1:31959956-31959975 | MS.gene51069:intron | 15.0% |
| !! | AAAAATATTTGTAGACAAAG+TGG | - | chr4.1:31960108-31960127 | None:intergenic | 20.0% |
| !! | AAAAGAGAAATGAATTTCAA+GGG | + | chr4.1:31960740-31960759 | MS.gene51069:CDS | 20.0% |
| !! | ATTAGAATTAAGTTCAAATC+AGG | + | chr4.1:31959825-31959844 | MS.gene51069:CDS | 20.0% |
| !! | CTTTATCAATGAATTTGATA+TGG | + | chr4.1:31959886-31959905 | MS.gene51069:intron | 20.0% |
| !! | TATTGAAATTGGAATTAGTT+TGG | + | chr4.1:31959967-31959986 | MS.gene51069:intron | 20.0% |
| !!! | ATTGAAATTGGAATTAGTTT+GGG | + | chr4.1:31959968-31959987 | MS.gene51069:intron | 20.0% |
| !!! | TTTTGTCTACAATATACAAA+GGG | + | chr4.1:31959921-31959940 | MS.gene51069:intron | 20.0% |
| !!! | TTTTTGTCTACAATATACAA+AGG | + | chr4.1:31959920-31959939 | MS.gene51069:intron | 20.0% |
| ! | AGATCAATTAAAAGAGAAGT+TGG | + | chr4.1:31960705-31960724 | MS.gene51069:CDS | 25.0% |
| ! | ATTGAATTTGATACTGTTAC+TGG | + | chr4.1:31960611-31960630 | MS.gene51069:intron | 25.0% |
| ! | GAAAAGAGAAATGAATTTCA+AGG | + | chr4.1:31960739-31960758 | MS.gene51069:CDS | 25.0% |
| !!! | AACTTCTCTTTTAATTGATC+TGG | - | chr4.1:31960706-31960725 | None:intergenic | 25.0% |
| !!! | TTTTCAGTATTGTTGCTTTA+AGG | + | chr4.1:31960203-31960222 | MS.gene51069:intron | 25.0% |
| TCAATTGTCAAAATACCGTA+TGG | - | chr4.1:31960338-31960357 | None:intergenic | 30.0% | |
| ATTTCAAGGGCTGTATGAAT+CGG | + | chr4.1:31960753-31960772 | MS.gene51069:CDS | 35.0% | |
| CTAGGTTAAACACCTTTACT+TGG | + | chr4.1:31960032-31960051 | MS.gene51069:intron | 35.0% | |
| CTCCTTTGACAAATTATCGT+GGG | + | chr4.1:31960911-31960930 | MS.gene51069:CDS | 35.0% | |
| GATCACGGTGATAAGTTAAA+CGG | + | chr4.1:31959853-31959872 | MS.gene51069:CDS | 35.0% | |
| GGTGTTTAACCTAGCAATAT+CGG | - | chr4.1:31960026-31960045 | None:intergenic | 35.0% | |
| TAAACAAATGCATCGTAGTC+TGG | + | chr4.1:31960146-31960165 | MS.gene51069:intron | 35.0% | |
| TCTCCTTTGACAAATTATCG+TGG | + | chr4.1:31960910-31960929 | MS.gene51069:CDS | 35.0% | |
| TGTATTCTCTCTCAAATACC+TGG | - | chr4.1:31960570-31960589 | None:intergenic | 35.0% | |
| ! | ATTGTGGTGATGATTCTTTC+TGG | + | chr4.1:31960250-31960269 | MS.gene51069:CDS | 35.0% |
| ! | TTGTGGTGATGATTCTTTCT+GGG | + | chr4.1:31960251-31960270 | MS.gene51069:CDS | 35.0% |
| ! | TTTGAAACAAAATGAGCACG+GGG | + | chr4.1:31960840-31960859 | MS.gene51069:CDS | 35.0% |
| !! | ATTCTTCACATGCTTTAGAG+TGG | + | chr4.1:31960431-31960450 | MS.gene51069:intron | 35.0% |
| !! | CTTTGAAACAAAATGAGCAC+GGG | + | chr4.1:31960839-31960858 | MS.gene51069:CDS | 35.0% |
| !! | GCTTTGAAACAAAATGAGCA+CGG | + | chr4.1:31960838-31960857 | MS.gene51069:CDS | 35.0% |
| !! | TGTATGTTGCTTTCAGATTG+TGG | + | chr4.1:31960234-31960253 | MS.gene51069:intron | 35.0% |
| !! | TTCTTCACATGCTTTAGAGT+GGG | + | chr4.1:31960432-31960451 | MS.gene51069:intron | 35.0% |
| ACACACAACTGTGATGTTAC+AGG | - | chr4.1:31960065-31960084 | None:intergenic | 40.0% | |
| ACAGGTTCGAATCCAAGTAA+AGG | - | chr4.1:31960047-31960066 | None:intergenic | 40.0% | |
| CAGTTGTGTGTATGAGTTTC+AGG | + | chr4.1:31960074-31960093 | MS.gene51069:intron | 40.0% | |
| GGTAAATGACTGTCAATTGC+AGG | - | chr4.1:31960964-31960983 | None:intergenic | 40.0% | |
| GTCAAAATACCGTATGGAAC+TGG | - | chr4.1:31960332-31960351 | None:intergenic | 40.0% | |
| TCAAATCAGGCTGATGATCA+CGG | + | chr4.1:31959838-31959857 | MS.gene51069:CDS | 40.0% | |
| TTGTGTGTATGAGTTTCAGG+TGG | + | chr4.1:31960077-31960096 | MS.gene51069:intron | 40.0% | |
| TTTAAACAATCAGTCCGTGC+TGG | + | chr4.1:31960515-31960534 | MS.gene51069:CDS | 40.0% | |
| ! | AGAAGGTCTTATAGCAGCAA+AGG | + | chr4.1:31960460-31960479 | MS.gene51069:CDS | 40.0% |
| ! | AGAGAAGTTGGTTGAAACAC+TGG | + | chr4.1:31960717-31960736 | MS.gene51069:CDS | 40.0% |
| ! | TGATGACAAGGTGTCAGATT+TGG | + | chr4.1:31960281-31960300 | MS.gene51069:CDS | 40.0% |
| ! | TGCCCACGATAATTTGTCAA+AGG | - | chr4.1:31960916-31960935 | None:intergenic | 40.0% |
| !! | GCATCGTAGTCTGGAATTTT+CGG | + | chr4.1:31960155-31960174 | MS.gene51069:intron | 40.0% |
| !! | TTAGTTTGGGTTAGTCCGTA+AGG | + | chr4.1:31959981-31960000 | MS.gene51069:intron | 40.0% |
| CTGGGATGATTCTGATGACA+AGG | + | chr4.1:31960269-31960288 | MS.gene51069:CDS | 45.0% | |
| GCACAGGAGGGATTCAATAT+TGG | + | chr4.1:31960491-31960510 | MS.gene51069:CDS | 45.0% | |
| TGTCGACAACAGATGCTCTT+AGG | + | chr4.1:31960785-31960804 | MS.gene51069:CDS | 45.0% | |
| TTGGCAATGCCGATATTGCT+AGG | + | chr4.1:31960014-31960033 | MS.gene51069:intron | 45.0% | |
| ! | ACAAGGTGTCAGATTTGGAC+AGG | + | chr4.1:31960286-31960305 | MS.gene51069:CDS | 45.0% |
| ! | CAAGGTGTCAGATTTGGACA+GGG | + | chr4.1:31960287-31960306 | MS.gene51069:CDS | 45.0% |
| !! | CTTTAGAGTGGGTATCGAGA+AGG | + | chr4.1:31960443-31960462 | MS.gene51069:intron | 45.0% |
| !! | GATGAGCAAAAGCGCTTCAA+AGG | - | chr4.1:31960681-31960700 | None:intergenic | 45.0% |
| !! | TATAGCTGGGGTGTTGTAAG+AGG | + | chr4.1:31960539-31960558 | MS.gene51069:CDS | 45.0% |
| AAAGGAAGCTTCTGCACAGG+AGG | + | chr4.1:31960478-31960497 | MS.gene51069:CDS | 50.0% | |
| AAGGAAGCTTCTGCACAGGA+GGG | + | chr4.1:31960479-31960498 | MS.gene51069:CDS | 50.0% | |
| AGCAAAGGAAGCTTCTGCAC+AGG | + | chr4.1:31960475-31960494 | MS.gene51069:CDS | 50.0% | |
| ATTTGGACAGGGAGTGGCAA+AGG | + | chr4.1:31960298-31960317 | MS.gene51069:CDS | 50.0% | |
| GCCAATTGAGCCAAGCCTTA+CGG | - | chr4.1:31959999-31960018 | None:intergenic | 50.0% | |
| TCCGTAAGGCTTGGCTCAAT+TGG | + | chr4.1:31959995-31960014 | MS.gene51069:intron | 50.0% | |
| TGTCAGATTTGGACAGGGAG+TGG | + | chr4.1:31960292-31960311 | MS.gene51069:CDS | 50.0% | |
| ! | GTGTTGTAAGAGGTGCATCC+AGG | + | chr4.1:31960549-31960568 | MS.gene51069:CDS | 50.0% |
| !! | AGTCCGTGCTGGTTATAGCT+GGG | + | chr4.1:31960526-31960545 | MS.gene51069:CDS | 50.0% |
| !! | TTGGGTTAGTCCGTAAGGCT+TGG | + | chr4.1:31959986-31960005 | MS.gene51069:intron | 50.0% |
| CACCCCAGCTATAACCAGCA+CGG | - | chr4.1:31960532-31960551 | None:intergenic | 55.0% | |
| GAGGCGTGACCAGTTCCATA+CGG | + | chr4.1:31960320-31960339 | MS.gene51069:CDS | 55.0% | |
| !! | CAGTCCGTGCTGGTTATAGC+TGG | + | chr4.1:31960525-31960544 | MS.gene51069:CDS | 55.0% |
| !! | GTCCGTGCTGGTTATAGCTG+GGG | + | chr4.1:31960527-31960546 | MS.gene51069:CDS | 55.0% |
| TGGACAGGGAGTGGCAAAGG+AGG | + | chr4.1:31960301-31960320 | MS.gene51069:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 31959765 | 31960996 | 31959765 | ID=MS.gene51069 |
| chr4.1 | mRNA | 31959765 | 31960996 | 31959765 | ID=MS.gene51069.t1;Parent=MS.gene51069 |
| chr4.1 | exon | 31959765 | 31959874 | 31959765 | ID=MS.gene51069.t1.exon1;Parent=MS.gene51069.t1 |
| chr4.1 | CDS | 31959765 | 31959874 | 31959765 | ID=cds.MS.gene51069.t1;Parent=MS.gene51069.t1 |
| chr4.1 | exon | 31960250 | 31960341 | 31960250 | ID=MS.gene51069.t1.exon2;Parent=MS.gene51069.t1 |
| chr4.1 | CDS | 31960250 | 31960341 | 31960250 | ID=cds.MS.gene51069.t1;Parent=MS.gene51069.t1 |
| chr4.1 | exon | 31960449 | 31960570 | 31960449 | ID=MS.gene51069.t1.exon3;Parent=MS.gene51069.t1 |
| chr4.1 | CDS | 31960449 | 31960570 | 31960449 | ID=cds.MS.gene51069.t1;Parent=MS.gene51069.t1 |
| chr4.1 | exon | 31960687 | 31960996 | 31960687 | ID=MS.gene51069.t1.exon4;Parent=MS.gene51069.t1 |
| chr4.1 | CDS | 31960687 | 31960996 | 31960687 | ID=cds.MS.gene51069.t1;Parent=MS.gene51069.t1 |
| Gene Sequence |
| Protein sequence |
