Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51710.t1 | XP_003598365.1 | 88.9 | 316 | 3 | 2 | 1 | 284 | 10 | 325 | 3.80E-150 | 540.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51710.t1 | G7IVH8 | 88.9 | 316 | 3 | 2 | 1 | 284 | 10 | 325 | 2.7e-150 | 540.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049350 | MS.gene51710 | 0.800219 | 1.60E-48 | -1.69E-46 |
| MS.gene049821 | MS.gene51710 | 0.824975 | 6.03E-54 | -1.69E-46 |
| MS.gene050901 | MS.gene51710 | 0.807717 | 4.41E-50 | -1.69E-46 |
| MS.gene051247 | MS.gene51710 | 0.808415 | 3.13E-50 | -1.69E-46 |
| MS.gene051700 | MS.gene51710 | 0.805443 | 1.33E-49 | -1.69E-46 |
| MS.gene051719 | MS.gene51710 | 0.855204 | 7.38E-62 | -1.69E-46 |
| MS.gene051720 | MS.gene51710 | 0.859992 | 2.82E-63 | -1.69E-46 |
| MS.gene052000 | MS.gene51710 | 0.810352 | 1.20E-50 | -1.69E-46 |
| MS.gene053122 | MS.gene51710 | 0.812817 | 3.50E-51 | -1.69E-46 |
| MS.gene053144 | MS.gene51710 | 0.81495 | 1.19E-51 | -1.69E-46 |
| MS.gene053581 | MS.gene51710 | 0.810362 | 1.20E-50 | -1.69E-46 |
| MS.gene054584 | MS.gene51710 | 0.816912 | 4.33E-52 | -1.69E-46 |
| MS.gene055465 | MS.gene51710 | 0.825396 | 4.80E-54 | -1.69E-46 |
| MS.gene055466 | MS.gene51710 | 0.822271 | 2.59E-53 | -1.69E-46 |
| MS.gene055468 | MS.gene51710 | 0.819319 | 1.24E-52 | -1.69E-46 |
| MS.gene055580 | MS.gene51710 | -0.819253 | 1.28E-52 | -1.69E-46 |
| MS.gene056005 | MS.gene51710 | 0.810873 | 9.28E-51 | -1.69E-46 |
| MS.gene056308 | MS.gene51710 | 0.801969 | 7.01E-49 | -1.69E-46 |
| MS.gene058625 | MS.gene51710 | 0.802285 | 6.03E-49 | -1.69E-46 |
| MS.gene05894 | MS.gene51710 | 0.805767 | 1.14E-49 | -1.69E-46 |
| MS.gene059625 | MS.gene51710 | 0.808196 | 3.49E-50 | -1.69E-46 |
| MS.gene060429 | MS.gene51710 | 0.807048 | 6.11E-50 | -1.69E-46 |
| MS.gene060519 | MS.gene51710 | 0.807651 | 4.55E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51710.t1 | MTR_3g010800 | 88.924 | 316 | 3 | 2 | 1 | 284 | 10 | 325 | 0.0 | 536 |
| MS.gene51710.t1 | MTR_3g062840 | 30.341 | 323 | 149 | 14 | 5 | 279 | 16 | 310 | 1.85e-22 | 95.1 |
| MS.gene51710.t1 | MTR_5g075750 | 31.466 | 232 | 138 | 9 | 48 | 279 | 91 | 301 | 2.36e-22 | 94.4 |
| MS.gene51710.t1 | MTR_7g074120 | 27.306 | 271 | 163 | 9 | 17 | 278 | 68 | 313 | 1.77e-14 | 72.8 |
| MS.gene51710.t1 | MTR_8g028165 | 26.360 | 239 | 150 | 9 | 40 | 275 | 79 | 294 | 1.56e-13 | 69.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51710.t1 | AT4G22900 | 40.979 | 327 | 134 | 9 | 5 | 278 | 12 | 332 | 6.52e-73 | 227 |
| MS.gene51710.t1 | AT4G11950 | 37.879 | 330 | 140 | 10 | 3 | 278 | 8 | 326 | 2.18e-65 | 207 |
| MS.gene51710.t1 | AT1G62981 | 35.922 | 309 | 142 | 13 | 19 | 279 | 41 | 341 | 1.28e-40 | 144 |
| MS.gene51710.t1 | AT1G62981 | 35.922 | 309 | 142 | 13 | 19 | 279 | 41 | 341 | 1.28e-40 | 144 |
| MS.gene51710.t1 | AT1G62981 | 35.676 | 185 | 102 | 8 | 107 | 279 | 29 | 208 | 1.67e-16 | 76.6 |
| MS.gene51710.t1 | AT3G08600 | 29.562 | 274 | 149 | 12 | 17 | 274 | 66 | 311 | 3.23e-12 | 66.2 |
Find 35 sgRNAs with CRISPR-Local
Find 50 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCTAAAACACATGGACATTT+TGG | 0.175170 | 3.4:-6810665 | MS.gene51710:CDS |
| TTCCATTGACTTAGTCTTAT+AGG | 0.189682 | 3.4:+6810596 | None:intergenic |
| AGTACTATTGGTGCTGCTTT+AGG | 0.264423 | 3.4:-6810560 | MS.gene51710:CDS |
| CCTTCAAAACTTGCACATAA+TGG | 0.317349 | 3.4:+6810743 | None:intergenic |
| CAACTTGTTTCTCCAATTGT+TGG | 0.336972 | 3.4:-6810914 | MS.gene51710:CDS |
| TAAGCTAAAATTCCAACAAT+TGG | 0.343703 | 3.4:+6810902 | None:intergenic |
| TTGTTGTCATTGGAATTTCA+TGG | 0.351140 | 3.4:-6811265 | MS.gene51710:CDS |
| TTCAACAACCAAGAAGAAAA+AGG | 0.352105 | 3.4:-6810779 | MS.gene51710:CDS |
| GTTGGTGTTGTTCTAGTTCC+AGG | 0.353242 | 3.4:+6810395 | None:intergenic |
| TTATGAAGAAGAAGCACTTC+AGG | 0.366104 | 3.4:-6810453 | MS.gene51710:CDS |
| GCATAGAGTATAATAATAAT+TGG | 0.373049 | 3.4:-6810979 | MS.gene51710:CDS |
| AAGGAGTTTCATCTTGGTAT+TGG | 0.399934 | 3.4:-6811049 | MS.gene51710:CDS |
| ATTGCAGTTGGGAGTACTAT+TGG | 0.400361 | 3.4:-6810572 | MS.gene51710:CDS |
| ACACAAACATTTGTTGCTAA+AGG | 0.423104 | 3.4:+6810689 | None:intergenic |
| GCACTTCAGGTTTCAATGGT+TGG | 0.434477 | 3.4:-6810440 | MS.gene51710:CDS |
| AGAAGCACTTCAGGTTTCAA+TGG | 0.442190 | 3.4:-6810444 | MS.gene51710:CDS |
| GCTTCAATTCCAAGTTGAAA+AGG | 0.444070 | 3.4:+6810845 | None:intergenic |
| AAATTAAAGGAGTTTCATCT+TGG | 0.452708 | 3.4:-6811055 | MS.gene51710:intron |
| AACATTACTCTTTCAATACA+TGG | 0.463603 | 3.4:+6811013 | None:intergenic |
| TTTCAACTTGGAATTGAAGC+TGG | 0.480245 | 3.4:-6810842 | MS.gene51710:CDS |
| TTAGACAAAGATTTCAAGAC+AGG | 0.484697 | 3.4:-6811172 | MS.gene51710:CDS |
| GTTCTAGTTCCAGGTGCAGT+AGG | 0.488529 | 3.4:+6810404 | None:intergenic |
| TGAATGTGTAATCTCTTATG+AGG | 0.490113 | 3.4:+6811200 | None:intergenic |
| GTCAATGGAAAATTGCAGTT+GGG | 0.491640 | 3.4:-6810583 | MS.gene51710:CDS |
| AGCCTATAAGACTAAGTCAA+TGG | 0.492496 | 3.4:-6810598 | MS.gene51710:CDS |
| GGTTAAGAAAAGGTCAAGAA+TGG | 0.493692 | 3.4:-6810495 | MS.gene51710:CDS |
| GAAGGTAGCTTCACTGATTG+TGG | 0.501570 | 3.4:+6811145 | None:intergenic |
| AGTCAATGGAAAATTGCAGT+TGG | 0.516447 | 3.4:-6810584 | MS.gene51710:CDS |
| AAGGTCAAGAATGGTTCAGA+TGG | 0.524303 | 3.4:-6810486 | MS.gene51710:CDS |
| TGGTTGTTAGGGTTAAGAAA+AGG | 0.555158 | 3.4:-6810505 | MS.gene51710:CDS |
| GTGAGAGCTCCTACTGCACC+TGG | 0.585262 | 3.4:-6810413 | MS.gene51710:CDS |
| GTTTGTGTTGCTAAAACACA+TGG | 0.605683 | 3.4:-6810674 | MS.gene51710:CDS |
| TAGTCATGTTGCTCAAGTGT+TGG | 0.655160 | 3.4:+6810377 | None:intergenic |
| ATGTGTAATCTCTTATGAGG+AGG | 0.665772 | 3.4:+6811203 | None:intergenic |
| TGCAGTAAACCTTATCAACG+AGG | 0.756012 | 3.4:+6810341 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GCATAGAGTATAATAATAAT+TGG | - | chr3.4:6810653-6810672 | MS.gene51710:CDS | 20.0% |
| ! | AAATTAAAGGAGTTTCATCT+TGG | - | chr3.4:6810577-6810596 | MS.gene51710:CDS | 25.0% |
| ! | AACATTACTCTTTCAATACA+TGG | + | chr3.4:6810622-6810641 | None:intergenic | 25.0% |
| ! | TAAGCTAAAATTCCAACAAT+TGG | + | chr3.4:6810733-6810752 | None:intergenic | 25.0% |
| AAGCTACCTTCAAATTTATC+AGG | - | chr3.4:6810499-6810518 | MS.gene51710:CDS | 30.0% | |
| AAGGTATGGTGCAAAATTAA+AGG | - | chr3.4:6810564-6810583 | MS.gene51710:CDS | 30.0% | |
| TAGATGCCTGATAAATTTGA+AGG | + | chr3.4:6810508-6810527 | None:intergenic | 30.0% | |
| TGAATGTGTAATCTCTTATG+AGG | + | chr3.4:6810435-6810454 | None:intergenic | 30.0% | |
| TTAGACAAAGATTTCAAGAC+AGG | - | chr3.4:6810460-6810479 | MS.gene51710:CDS | 30.0% | |
| TTAGATGTGGAAGTTTAACA+AGG | - | chr3.4:6810545-6810564 | MS.gene51710:CDS | 30.0% | |
| TTCAACAACCAAGAAGAAAA+AGG | - | chr3.4:6810853-6810872 | MS.gene51710:CDS | 30.0% | |
| TTGTTGTCATTGGAATTTCA+TGG | - | chr3.4:6810367-6810386 | MS.gene51710:CDS | 30.0% | |
| ! | ACACAAACATTTGTTGCTAA+AGG | + | chr3.4:6810946-6810965 | None:intergenic | 30.0% |
| ! | AGTAGTAATCCTTTTCAACT+TGG | - | chr3.4:6810778-6810797 | MS.gene51710:CDS | 30.0% |
| ! | CTTTGATACCTTTTTCTTCT+TGG | + | chr3.4:6810864-6810883 | None:intergenic | 30.0% |
| ! | GACATTTTCATTGTTGTCAT+TGG | - | chr3.4:6810357-6810376 | MS.gene51710:CDS | 30.0% |
| ! | TTTTGTAGCAATGGTTGTTA+GGG | - | chr3.4:6811116-6811135 | MS.gene51710:intron | 30.0% |
| ! | TTTTTGTAGCAATGGTTGTT+AGG | - | chr3.4:6811115-6811134 | MS.gene51710:intron | 30.0% |
| !! | TTCCATTGACTTAGTCTTAT+AGG | + | chr3.4:6811039-6811058 | None:intergenic | 30.0% |
| !!! | AGGTTTACTTTTTGTAGCAA+TGG | - | chr3.4:6811107-6811126 | MS.gene51710:intron | 30.0% |
| AAGGAGTTTCATCTTGGTAT+TGG | - | chr3.4:6810583-6810602 | MS.gene51710:CDS | 35.0% | |
| AGCCTATAAGACTAAGTCAA+TGG | - | chr3.4:6811034-6811053 | MS.gene51710:CDS | 35.0% | |
| AGTCAATGGAAAATTGCAGT+TGG | - | chr3.4:6811048-6811067 | MS.gene51710:CDS | 35.0% | |
| ATGTGTAATCTCTTATGAGG+AGG | + | chr3.4:6810432-6810451 | None:intergenic | 35.0% | |
| CCTTCAAAACTTGCACATAA+TGG | + | chr3.4:6810892-6810911 | None:intergenic | 35.0% | |
| GATACTGTGAGATTTAGATG+TGG | - | chr3.4:6810532-6810551 | MS.gene51710:CDS | 35.0% | |
| GGTTAAGAAAAGGTCAAGAA+TGG | - | chr3.4:6811137-6811156 | MS.gene51710:intron | 35.0% | |
| GTCAATGGAAAATTGCAGTT+GGG | - | chr3.4:6811049-6811068 | MS.gene51710:CDS | 35.0% | |
| TGGTTGTTAGGGTTAAGAAA+AGG | - | chr3.4:6811127-6811146 | MS.gene51710:intron | 35.0% | |
| TGTGGAAGTTTAACAAGGTA+TGG | - | chr3.4:6810550-6810569 | MS.gene51710:CDS | 35.0% | |
| TTTCAACTTGGAATTGAAGC+TGG | - | chr3.4:6810790-6810809 | MS.gene51710:CDS | 35.0% | |
| ! | CAACTTGTTTCTCCAATTGT+TGG | - | chr3.4:6810718-6810737 | MS.gene51710:CDS | 35.0% |
| ! | GCTAAAACACATGGACATTT+TGG | - | chr3.4:6810967-6810986 | MS.gene51710:CDS | 35.0% |
| ! | GCTTCAATTCCAAGTTGAAA+AGG | + | chr3.4:6810790-6810809 | None:intergenic | 35.0% |
| ! | GTTTGTGTTGCTAAAACACA+TGG | - | chr3.4:6810958-6810977 | MS.gene51710:CDS | 35.0% |
| !! | CCATTATGTGCAAGTTTTGA+AGG | - | chr3.4:6810889-6810908 | MS.gene51710:CDS | 35.0% |
| !! | GCTTTAGGTGCTTTTCTTTT+AGG | - | chr3.4:6811087-6811106 | MS.gene51710:intron | 35.0% |
| !! | TTATGAAGAAGAAGCACTTC+AGG | - | chr3.4:6811179-6811198 | MS.gene51710:CDS | 35.0% |
| !!! | AACACATGGACATTTTGGTT+TGG | - | chr3.4:6810972-6810991 | MS.gene51710:CDS | 35.0% |
| !!! | TGTGCAAGTTTTGAAGGAAA+TGG | - | chr3.4:6810895-6810914 | MS.gene51710:CDS | 35.0% |
| AAGGTCAAGAATGGTTCAGA+TGG | - | chr3.4:6811146-6811165 | MS.gene51710:intron | 40.0% | |
| TAGTCATGTTGCTCAAGTGT+TGG | + | chr3.4:6811258-6811277 | None:intergenic | 40.0% | |
| !! | AGAAGCACTTCAGGTTTCAA+TGG | - | chr3.4:6811188-6811207 | MS.gene51710:CDS | 40.0% |
| !! | AGTACTATTGGTGCTGCTTT+AGG | - | chr3.4:6811072-6811091 | MS.gene51710:intron | 40.0% |
| !! | ATTGCAGTTGGGAGTACTAT+TGG | - | chr3.4:6811060-6811079 | MS.gene51710:intron | 40.0% |
| ! | GAAGGTAGCTTCACTGATTG+TGG | + | chr3.4:6810490-6810509 | None:intergenic | 45.0% |
| ! | GCACTTCAGGTTTCAATGGT+TGG | - | chr3.4:6811192-6811211 | MS.gene51710:CDS | 45.0% |
| !! | GTTGGTGTTGTTCTAGTTCC+AGG | + | chr3.4:6811240-6811259 | None:intergenic | 45.0% |
| !! | GTTCTAGTTCCAGGTGCAGT+AGG | + | chr3.4:6811231-6811250 | None:intergenic | 50.0% |
| GTGAGAGCTCCTACTGCACC+TGG | - | chr3.4:6811219-6811238 | MS.gene51710:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 6810355 | 6811299 | 6810355 | ID=MS.gene51710 |
| chr3.4 | mRNA | 6810355 | 6811299 | 6810355 | ID=MS.gene51710.t1;Parent=MS.gene51710 |
| chr3.4 | exon | 6811159 | 6811299 | 6811159 | ID=MS.gene51710.t1.exon1;Parent=MS.gene51710.t1 |
| chr3.4 | CDS | 6811159 | 6811299 | 6811159 | ID=cds.MS.gene51710.t1;Parent=MS.gene51710.t1 |
| chr3.4 | exon | 6810355 | 6811068 | 6810355 | ID=MS.gene51710.t1.exon2;Parent=MS.gene51710.t1 |
| chr3.4 | CDS | 6810355 | 6811068 | 6810355 | ID=cds.MS.gene51710.t1;Parent=MS.gene51710.t1 |
| Gene Sequence |
| Protein sequence |
