AlfalfaGEDB | MS.gene52721

M. sativa cultivar XinJiangDaYe / MS.gene52721

KEGG: K03094
GO: GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009987 GO:0019538 GO:0019941 GO:0043170 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0051603 GO:0071704 GO:1901564 GO:1901575
PFAM: PF01466.22 PF03931.18

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene52721.t1	RHN48019.1	92.3	156	12	0	1	156	1	156	1.10E-74	289.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene52721.t1	Q39255	74.2	159	35	1	4	156	2	160	2.4e-62	239.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene52721.t1	G7KSZ5	91.7	156	13	0	1	156	1	156	7.8e-75	289.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene25469	MS.gene52721	PPI
MS.gene79720	MS.gene52721	PPI
MS.gene98172	MS.gene52721	PPI
MS.gene52721	MS.gene002824	PPI
MS.gene52721	MS.gene004511	PPI
MS.gene24959	MS.gene52721	PPI
MS.gene52721	MS.gene25471	PPI
MS.gene047254	MS.gene52721	PPI
MS.gene52721	MS.gene33667	PPI
MS.gene33411	MS.gene52721	PPI
MS.gene79719	MS.gene52721	PPI
MS.gene34691	MS.gene52721	PPI
MS.gene00939	MS.gene52721	PPI
MS.gene52721	MS.gene055864	PPI
MS.gene35971	MS.gene52721	PPI
MS.gene59610	MS.gene52721	PPI
MS.gene92141	MS.gene52721	PPI
MS.gene068281	MS.gene52721	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene52721.t1	MTR_7g021400	91.667	156	13	0	1	156	1	156	2.43e-99	282
MS.gene52721.t1	MTR_7g094150	91.667	156	13	0	1	156	1	156	2.69e-98	280
MS.gene52721.t1	MTR_7g094170	91.667	156	13	0	1	156	1	156	5.37e-97	276
MS.gene52721.t1	MTR_7g021380	87.821	156	19	0	1	156	1	156	1.80e-92	265
MS.gene52721.t1	MTR_5g022710	81.818	154	28	0	3	156	2	155	2.01e-88	255
MS.gene52721.t1	MTR_7g094120	77.707	157	33	1	2	156	3	159	2.80e-88	254
MS.gene52721.t1	MTR_5g022730	81.818	154	28	0	3	156	2	155	4.33e-88	254
MS.gene52721.t1	MTR_4g032400	78.846	156	32	1	2	156	8	163	1.32e-86	251
MS.gene52721.t1	MTR_4g032450	72.611	157	42	1	1	156	19	175	7.50e-82	239
MS.gene52721.t1	MTR_3g110048	73.418	158	40	1	1	156	1	158	1.51e-81	238
MS.gene52721.t1	MTR_7g094280	75.316	158	37	2	1	156	1	158	1.53e-81	238
MS.gene52721.t1	MTR_8g056060	70.701	157	45	1	1	156	1	157	1.57e-76	225
MS.gene52721.t1	MTR_8g468480	68.387	155	48	1	3	156	2	156	1.60e-73	217
MS.gene52721.t1	MTR_7g094310	70.253	158	35	3	1	156	1	148	9.12e-72	213
MS.gene52721.t1	MTR_5g085030	65.584	154	52	1	3	155	2	155	1.02e-68	205
MS.gene52721.t1	MTR_4g032500	66.026	156	46	2	2	156	3	152	5.89e-68	203
MS.gene52721.t1	MTR_4g032510	67.516	157	43	3	2	156	3	153	2.53e-67	201
MS.gene52721.t1	MTR_4g032460	69.032	155	41	2	3	156	2	150	4.67e-67	201
MS.gene52721.t1	MTR_4g033455	63.636	154	56	0	3	156	7	160	7.71e-67	200
MS.gene52721.t1	MTR_4g017860	56.410	195	42	3	5	156	26	220	4.80e-65	198
MS.gene52721.t1	MTR_4g033460	63.380	142	52	0	1	142	5	146	1.22e-61	190
MS.gene52721.t1	MTR_4g033460	63.441	93	26	1	41	133	161	245	6.62e-32	114
MS.gene52721.t1	MTR_4g021390	59.355	155	60	1	2	156	4	155	9.82e-59	180
MS.gene52721.t1	MTR_1g099870	84.314	102	14	1	57	156	2	103	1.14e-58	177
MS.gene52721.t1	MTR_5g082340	61.290	155	48	2	3	156	2	145	4.25e-58	177
MS.gene52721.t1	MTR_7g094320	57.325	157	26	2	1	156	1	117	2.46e-51	159
MS.gene52721.t1	MTR_8g089730	69.106	123	21	1	34	156	1	106	5.22e-51	158
MS.gene52721.t1	MTR_4g032420	60.630	127	35	3	31	156	38	150	1.26e-46	149
MS.gene52721.t1	MTR_7g006370	58.156	141	50	2	2	142	4	135	7.04e-46	146
MS.gene52721.t1	MTR_4g032470	51.370	146	27	2	12	156	32	134	3.60e-43	139
MS.gene52721.t1	MTR_7g094410	52.830	159	27	3	1	114	1	156	5.39e-43	140
MS.gene52721.t1	MTR_4g131980	65.546	119	35	3	3	119	2	116	8.29e-42	136
MS.gene52721.t1	MTR_4g127240	66.667	102	28	3	41	140	27	124	1.76e-40	132
MS.gene52721.t1	MTR_8g056090	51.282	156	43	6	1	154	1	125	2.71e-38	127
MS.gene52721.t1	MTR_7g094360	44.149	188	52	4	1	135	1	188	2.82e-38	129
MS.gene52721.t1	MTR_7g094350	44.681	188	50	4	1	135	1	187	5.25e-38	128
MS.gene52721.t1	MTR_4g119422	48.966	145	67	2	2	144	227	366	5.85e-34	122
MS.gene52721.t1	MTR_1g112590	36.129	155	83	2	8	146	1	155	3.56e-29	105
MS.gene52721.t1	MTR_7g071200	71.014	69	19	1	89	156	4	72	2.47e-28	100
MS.gene52721.t1	MTR_2g066010	38.158	152	80	5	8	155	20	161	5.63e-25	95.1
MS.gene52721.t1	MTR_2g065720	36.478	159	87	5	1	155	30	178	4.26e-24	93.2
MS.gene52721.t1	MTR_4g032520	40.972	144	43	3	29	154	1	120	1.04e-23	90.5
MS.gene52721.t1	MTR_4g032540	66.667	63	20	1	90	151	70	132	2.81e-23	89.0
MS.gene52721.t1	MTR_5g096950	36.538	156	84	5	1	155	1	142	3.37e-21	84.3
MS.gene52721.t1	MTR_2g018980	35.948	153	79	5	8	156	3	140	4.42e-21	84.0
MS.gene52721.t1	MTR_1g042510	33.974	156	65	4	8	146	1	135	4.42e-20	84.3
MS.gene52721.t1	MTR_1g112570	38.129	139	61	5	8	128	1	132	5.28e-20	84.0
MS.gene52721.t1	MTR_5g078680	32.692	156	67	4	8	146	1	135	3.51e-18	79.0
MS.gene52721.t1	MTR_6g053620	36.190	105	61	2	43	146	56	155	3.95e-16	74.3
MS.gene52721.t1	MTR_6g053620	36.190	105	61	2	43	146	56	155	5.10e-16	73.9
MS.gene52721.t1	MTR_7g077810	33.793	145	84	4	8	146	18	156	6.82e-16	73.6
MS.gene52721.t1	MTR_8g007195	32.857	140	82	4	10	143	13	146	1.11e-15	72.8
MS.gene52721.t1	MTR_8g007205	37.000	100	56	2	49	147	58	151	2.98e-15	71.2
MS.gene52721.t1	MTR_8g007185	37.000	100	56	2	49	147	58	151	3.30e-15	71.2
MS.gene52721.t1	MTR_2g100830	29.861	144	87	4	10	146	13	149	1.46e-13	67.0
MS.gene52721.t1	MTR_8g030670	35.354	99	57	2	49	146	64	156	1.71e-13	67.0
MS.gene52721.t1	MTR_4g131910	39.286	112	58	2	5	112	133	238	3.47e-13	65.1
MS.gene52721.t1	MTR_2g100825	29.870	154	91	5	1	146	4	148	1.13e-12	64.3
MS.gene52721.t1	MTR_3g061700	34.737	95	55	2	53	146	1	89	3.15e-12	62.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene52721.t1	AT1G20140	69.939	163	42	1	1	156	1	163	5.74e-82	239
MS.gene52721.t1	AT5G42190	71.765	170	32	1	3	156	2	171	9.54e-81	236
MS.gene52721.t1	AT1G75950	74.214	159	35	1	4	156	2	160	4.49e-80	234
MS.gene52721.t1	AT2G25700	67.273	165	43	2	1	156	1	163	1.91e-77	227
MS.gene52721.t1	AT4G34210	67.320	153	48	1	4	156	2	152	1.60e-73	217
MS.gene52721.t1	AT4G34470	66.234	154	48	2	4	156	2	152	1.02e-71	213
MS.gene52721.t1	AT3G21850	67.097	155	46	3	4	156	2	153	1.57e-68	204
MS.gene52721.t1	AT3G60010	66.234	154	49	2	5	156	2	154	2.05e-66	199
MS.gene52721.t1	AT3G21860	64.286	154	51	2	4	156	2	152	2.22e-66	199
MS.gene52721.t1	AT2G03170	61.039	154	53	2	4	156	2	149	3.38e-61	186
MS.gene52721.t1	AT3G21830	61.039	154	56	2	4	156	2	152	7.68e-59	180
MS.gene52721.t1	AT2G03190	54.819	166	62	3	4	156	2	167	2.75e-57	177
MS.gene52721.t1	AT2G03160	51.323	189	56	5	4	156	2	190	6.51e-56	174
MS.gene52721.t1	AT3G60020	53.797	158	61	3	5	156	2	153	3.39e-54	168
MS.gene52721.t1	AT3G25650	59.036	166	55	5	4	156	2	167	8.28e-54	168
MS.gene52721.t1	AT1G10230	55.484	155	66	2	4	155	2	156	1.06e-51	162
MS.gene52721.t1	AT2G20160	52.941	153	66	2	4	155	2	149	5.45e-51	160
MS.gene52721.t1	AT3G21840	58.824	119	45	2	4	121	2	117	4.50e-38	126
MS.gene52721.t1	AT3G53060	62.791	86	25	3	29	113	1	80	1.09e-29	103
MS.gene52721.t1	AT3G61415	31.724	145	87	5	8	146	18	156	7.81e-14	67.8
MS.gene52721.t1	AT3G61415	31.724	145	87	5	8	146	18	156	8.18e-14	67.8
MS.gene52721.t1	AT2G45950	29.655	145	90	5	8	146	18	156	2.30e-13	66.6
MS.gene52721.t1	AT2G45950	29.655	145	90	5	8	146	18	156	2.30e-13	66.6
MS.gene52721.t1	AT2G45950	29.655	145	90	5	8	146	18	156	2.78e-13	66.2
MS.gene52721.t1	AT2G45950	29.655	145	90	5	8	146	18	156	2.78e-13	66.2
MS.gene52721.t1	AT2G45950	29.655	145	90	5	8	146	18	156	2.78e-13	66.2

Find 37 sgRNAs with CRISPR-Local

Find 38 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CAGATGACGATCTTAATAAT+TGG	0.177735	7.2:+15298826	MS.gene52721:CDS
TAACGTGACCGGTCAGATAT+TGG	0.310767	7.2:+15298743	MS.gene52721:CDS
CGAATTTCTGCTTCTTCCTC+TGG	0.323200	7.2:-15299023	None:intergenic
TGACCGGTCACGTTAGGAAC+TGG	0.377814	7.2:-15298735	None:intergenic
AGATGACGATCTTAATAATT+GGG	0.397073	7.2:+15298827	MS.gene52721:CDS
CTTGAATATCAAAAGTCTAC+TGG	0.408664	7.2:+15298911	MS.gene52721:CDS
ACTCTTGAGAGTGATCTTCT+TGG	0.430026	7.2:-15298616	None:intergenic
AATATCTGACCGGTCACGTT+AGG	0.431469	7.2:-15298741	None:intergenic
TGATCATGTCTGCCACAGTC+TGG	0.435485	7.2:-15298944	None:intergenic
CTTGAGAGTGATCTTCTTGG+TGG	0.435670	7.2:-15298613	None:intergenic
AACCTCAAAGGTTTCGTTGT+CGG	0.443158	7.2:-15298640	None:intergenic
AGGAACTGGGATTCCGTTGT+CGG	0.448853	7.2:-15298721	None:intergenic
GATTCAAGTGCCACAGCCTC+GGG	0.453635	7.2:-15298663	None:intergenic
TTCCGACAACGAAACCTTTG+AGG	0.470820	7.2:+15298638	MS.gene52721:CDS
CGATTCAAGTGCCACAGCCT+CGG	0.485929	7.2:-15298664	None:intergenic
TGAATTCGTGAAAGTTGATC+AGG	0.495410	7.2:+15298854	MS.gene52721:CDS
CATGTCTGCCACAGTCTGGC+AGG	0.496105	7.2:-15298940	None:intergenic
CTTTGAGGTTCCCGAGGCTG+TGG	0.504428	7.2:+15298653	MS.gene52721:CDS
GACCGGTCACGTTAGGAACT+GGG	0.517391	7.2:-15298734	None:intergenic
CTGTGGCAGACATGATCAAA+GGG	0.535149	7.2:+15298949	MS.gene52721:CDS
AATTCGTAAGGAAAACCAAT+GGG	0.541324	7.2:+15299040	MS.gene52721:CDS
AATTTCTGACGAATTTGCTC+CGG	0.551209	7.2:-15298978	None:intergenic
ATCCCAGTTCCTAACGTGAC+CGG	0.555556	7.2:+15298732	MS.gene52721:CDS
TGATTCATTCAAAAGCCCAT+TGG	0.556289	7.2:-15299055	None:intergenic
CACAGCCTCGGGAACCTCAA+AGG	0.556606	7.2:-15298652	None:intergenic
CACCTTCGCCAATATCTGAC+CGG	0.557441	7.2:-15298751	None:intergenic
GAGGACAATTGCACCGACAA+CGG	0.563633	7.2:+15298708	MS.gene52721:CDS
TTATTAAGATCGTCATCTGA+AGG	0.570016	7.2:-15298822	None:intergenic
GGAAGAAGCAGAAATTCGTA+AGG	0.575149	7.2:+15299028	MS.gene52721:CDS
GACGATCAAGCACATGATCG+AGG	0.578464	7.2:+15298689	MS.gene52721:CDS
ACTGTGGCAGACATGATCAA+AGG	0.589851	7.2:+15298948	MS.gene52721:CDS
CGAAACCTTTGAGGTTCCCG+AGG	0.621372	7.2:+15298647	MS.gene52721:CDS
AAATTCGTAAGGAAAACCAA+TGG	0.621698	7.2:+15299039	MS.gene52721:CDS
GACCGGTCAGATATTGGCGA+AGG	0.633811	7.2:+15298749	MS.gene52721:CDS
CAAGAATGATTTCACTCCAG+AGG	0.644538	7.2:+15299007	MS.gene52721:CDS
GGATCTTACCTGCCAGACTG+TGG	0.651686	7.2:+15298932	MS.gene52721:CDS
CATGATCAAAGGGAAGACAC+CGG	0.704151	7.2:+15298959	MS.gene52721:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AGATGACGATCTTAATAATT+GGG	+	chr7.2:15298827-15298846	MS.gene52721:CDS	25.0%
	AAATTCGTAAGGAAAACCAA+TGG	+	chr7.2:15299039-15299058	MS.gene52721:CDS	30.0%
	AATTCGTAAGGAAAACCAAT+GGG	+	chr7.2:15299040-15299059	MS.gene52721:CDS	30.0%
	CAGATGACGATCTTAATAAT+TGG	+	chr7.2:15298826-15298845	MS.gene52721:CDS	30.0%
	CTTGAATATCAAAAGTCTAC+TGG	+	chr7.2:15298911-15298930	MS.gene52721:CDS	30.0%
	TTATTAAGATCGTCATCTGA+AGG	-	chr7.2:15298825-15298844	None:intergenic	30.0%
!	AATTTCTGACGAATTTGCTC+CGG	-	chr7.2:15298981-15299000	None:intergenic	35.0%
!	GTCATCTGAAGGTTTTTCAT+CGG	-	chr7.2:15298814-15298833	None:intergenic	35.0%
!	TGAATTCGTGAAAGTTGATC+AGG	+	chr7.2:15298854-15298873	MS.gene52721:CDS	35.0%
	AACCTCAAAGGTTTCGTTGT+CGG	-	chr7.2:15298643-15298662	None:intergenic	40.0%
	ACTCTTGAGAGTGATCTTCT+TGG	-	chr7.2:15298619-15298638	None:intergenic	40.0%
	CAAGAATGATTTCACTCCAG+AGG	+	chr7.2:15299007-15299026	MS.gene52721:CDS	40.0%
	GGAAGAAGCAGAAATTCGTA+AGG	+	chr7.2:15299028-15299047	MS.gene52721:CDS	40.0%
!	AGGTTTTTCATCGGAACTCA+CGG	-	chr7.2:15298805-15298824	None:intergenic	40.0%
	AATATCTGACCGGTCACGTT+AGG	-	chr7.2:15298744-15298763	None:intergenic	45.0%
	ACTGTGGCAGACATGATCAA+AGG	+	chr7.2:15298948-15298967	MS.gene52721:CDS	45.0%
	CATGATCAAAGGGAAGACAC+CGG	+	chr7.2:15298959-15298978	MS.gene52721:CDS	45.0%
	CGAATTTCTGCTTCTTCCTC+TGG	-	chr7.2:15299026-15299045	None:intergenic	45.0%
	CTGTGGCAGACATGATCAAA+GGG	+	chr7.2:15298949-15298968	MS.gene52721:CDS	45.0%
	CTTGAGAGTGATCTTCTTGG+TGG	-	chr7.2:15298616-15298635	None:intergenic	45.0%
	TAACGTGACCGGTCAGATAT+TGG	+	chr7.2:15298743-15298762	MS.gene52721:CDS	45.0%
	TTCCGACAACGAAACCTTTG+AGG	+	chr7.2:15298638-15298657	MS.gene52721:CDS	45.0%
	AGGAACTGGGATTCCGTTGT+CGG	-	chr7.2:15298724-15298743	None:intergenic	50.0%
	ATCCCAGTTCCTAACGTGAC+CGG	+	chr7.2:15298732-15298751	MS.gene52721:CDS	50.0%
	CACCTTCGCCAATATCTGAC+CGG	-	chr7.2:15298754-15298773	None:intergenic	50.0%
	GACGATCAAGCACATGATCG+AGG	+	chr7.2:15298689-15298708	MS.gene52721:CDS	50.0%
	TGATCATGTCTGCCACAGTC+TGG	-	chr7.2:15298947-15298966	None:intergenic	50.0%
!	GAGGACAATTGCACCGACAA+CGG	+	chr7.2:15298708-15298727	MS.gene52721:CDS	50.0%
	CGATTCAAGTGCCACAGCCT+CGG	-	chr7.2:15298667-15298686	None:intergenic	55.0%
	GACCGGTCACGTTAGGAACT+GGG	-	chr7.2:15298737-15298756	None:intergenic	55.0%
	GACCGGTCAGATATTGGCGA+AGG	+	chr7.2:15298749-15298768	MS.gene52721:CDS	55.0%
	GATTCAAGTGCCACAGCCTC+GGG	-	chr7.2:15298666-15298685	None:intergenic	55.0%
	GGATCTTACCTGCCAGACTG+TGG	+	chr7.2:15298932-15298951	MS.gene52721:CDS	55.0%
	TGACCGGTCACGTTAGGAAC+TGG	-	chr7.2:15298738-15298757	None:intergenic	55.0%
!!	CGAAACCTTTGAGGTTCCCG+AGG	+	chr7.2:15298647-15298666	MS.gene52721:CDS	55.0%
	CACAGCCTCGGGAACCTCAA+AGG	-	chr7.2:15298655-15298674	None:intergenic	60.0%
	CATGTCTGCCACAGTCTGGC+AGG	-	chr7.2:15298943-15298962	None:intergenic	60.0%
!!	CTTTGAGGTTCCCGAGGCTG+TGG	+	chr7.2:15298653-15298672	MS.gene52721:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.2	gene	15298603	15299073	15298603	ID=MS.gene52721
chr7.2	mRNA	15298603	15299073	15298603	ID=MS.gene52721.t1;Parent=MS.gene52721
chr7.2	exon	15298603	15299073	15298603	ID=MS.gene52721.t1.exon1;Parent=MS.gene52721.t1
chr7.2	CDS	15298603	15299073	15298603	ID=cds.MS.gene52721.t1;Parent=MS.gene52721.t1

Gene Sequence

>MS.gene52721

ATGTCTTCAACCACCAAGAAGATCACTCTCAAGAGTTCCGACAACGAAACCTTTGAGGTTCCCGAGGCTGTGGCACTTGAATCGCAGACGATCAAGCACATGATCGAGGACAATTGCACCGACAACGGAATCCCAGTTCCTAACGTGACCGGTCAGATATTGGCGAAGGTGATTGAATACTGTAAGAAGCATGTTGATGCCGTGAGTTCCGATGAAAAACCTTCAGATGACGATCTTAATAATTGGGATGCTGAATTCGTGAAAGTTGATCAGGCTACGCTTTTCGATCTCATTCTTGCTGCAAATTACTTGAATATCAAAAGTCTACTGGATCTTACCTGCCAGACTGTGGCAGACATGATCAAAGGGAAGACACCGGAGCAAATTCGTCAGAAATTTAACATCAAGAATGATTTCACTCCAGAGGAAGAAGCAGAAATTCGTAAGGAAAACCAATGGGCTTTTGAATGA

Protein sequence

>MS.gene52721.t1

MSSTTKKITLKSSDNETFEVPEAVALESQTIKHMIEDNCTDNGIPVPNVTGQILAKVIEYCKKHVDAVSSDEKPSDDDLNNWDAEFVKVDQATLFDLILAANYLNIKSLLDLTCQTVADMIKGKTPEQIRQKFNIKNDFTPEEEAEIRKENQWAFE