Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53319.t1 | XP_004141696.1 | 97.7 | 88 | 2 | 0 | 1 | 88 | 14 | 101 | 1.40E-35 | 159.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53319.t1 | Q9SKI2 | 94.3 | 87 | 5 | 0 | 1 | 87 | 15 | 101 | 2.0e-35 | 150.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53319.t1 | A0A0A0K7U7 | 97.7 | 88 | 2 | 0 | 1 | 88 | 14 | 101 | 9.9e-36 | 159.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049159 | MS.gene53319 | 0.806678 | 7.32E-50 | -1.69E-46 |
| MS.gene049378 | MS.gene53319 | 0.808424 | 3.12E-50 | -1.69E-46 |
| MS.gene050226 | MS.gene53319 | 0.811142 | 8.12E-51 | -1.69E-46 |
| MS.gene050227 | MS.gene53319 | 0.811878 | 5.62E-51 | -1.69E-46 |
| MS.gene050228 | MS.gene53319 | 0.82106 | 4.94E-53 | -1.69E-46 |
| MS.gene050442 | MS.gene53319 | 0.829699 | 4.45E-55 | -1.69E-46 |
| MS.gene050719 | MS.gene53319 | 0.812836 | 3.47E-51 | -1.69E-46 |
| MS.gene050721 | MS.gene53319 | 0.814201 | 1.74E-51 | -1.69E-46 |
| MS.gene052176 | MS.gene53319 | 0.80743 | 5.07E-50 | -1.69E-46 |
| MS.gene052333 | MS.gene53319 | 0.804344 | 2.26E-49 | -1.69E-46 |
| MS.gene052426 | MS.gene53319 | 0.806382 | 8.45E-50 | -1.69E-46 |
| MS.gene054468 | MS.gene53319 | 0.812441 | 4.23E-51 | -1.69E-46 |
| MS.gene054930 | MS.gene53319 | 0.817315 | 3.51E-52 | -1.69E-46 |
| MS.gene055701 | MS.gene53319 | 0.803297 | 3.73E-49 | -1.69E-46 |
| MS.gene056506 | MS.gene53319 | 0.837018 | 6.66E-57 | -1.69E-46 |
| MS.gene057026 | MS.gene53319 | 0.816273 | 6.02E-52 | -1.69E-46 |
| MS.gene057194 | MS.gene53319 | 0.818625 | 1.78E-52 | -1.69E-46 |
| MS.gene05761 | MS.gene53319 | 0.808275 | 3.36E-50 | -1.69E-46 |
| MS.gene059249 | MS.gene53319 | 0.816333 | 5.84E-52 | -1.69E-46 |
| MS.gene060011 | MS.gene53319 | 0.814243 | 1.70E-51 | -1.69E-46 |
| MS.gene060312 | MS.gene53319 | 0.804502 | 2.10E-49 | -1.69E-46 |
| MS.gene060322 | MS.gene53319 | 0.852551 | 4.29E-61 | -1.69E-46 |
| MS.gene06052 | MS.gene53319 | 0.807759 | 4.32E-50 | -1.69E-46 |
| MS.gene060683 | MS.gene53319 | 0.803714 | 3.06E-49 | -1.69E-46 |
| MS.gene060685 | MS.gene53319 | 0.800291 | 1.54E-48 | -1.69E-46 |
| MS.gene060998 | MS.gene53319 | 0.851353 | 9.38E-61 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 32 sgRNAs with CRISPR-Local
Find 85 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGGTGTTGCTTTGAGAATTC+AGG | 0.273393 | 8.4:-73404818 | MS.gene53319:intron |
| CAAGCTTAAATCACAACTTC+AGG | 0.310848 | 8.4:-73404839 | MS.gene53319:CDS |
| AAATACCATTTGTCCTTGTT+TGG | 0.313748 | 8.4:+73405581 | None:intergenic |
| TTAGGGTGCTGTGAGGGTTA+TGG | 0.358019 | 8.4:-73404905 | MS.gene53319:intron |
| GGAACTTGTTTCTGAGGTAA+TGG | 0.405606 | 8.4:-73404205 | MS.gene53319:CDS |
| GAACTTGTTTCTGAGGTAAT+GGG | 0.408793 | 8.4:-73404204 | MS.gene53319:CDS |
| AAGCAATGGGTCAAATGAAT+AGG | 0.409703 | 8.4:-73404290 | MS.gene53319:CDS |
| TGCATTATCTTCTGTAAAGA+AGG | 0.410358 | 8.4:+73404255 | None:intergenic |
| GCAATTGATGATGCTTTAGA+AGG | 0.423361 | 8.4:-73404177 | MS.gene53319:CDS |
| CTGAAATCTACTCAAGCAAT+GGG | 0.427818 | 8.4:-73404336 | MS.gene53319:CDS |
| GAATGCCAAACAAGGACAAA+TGG | 0.480489 | 8.4:-73405586 | MS.gene53319:intron |
| AAAGGAGTTACTAAAGCAAT+GGG | 0.498174 | 8.4:-73404303 | MS.gene53319:CDS |
| AGTCTATTAGAGAAATTGAA+AGG | 0.508512 | 8.4:-73405662 | MS.gene53319:CDS |
| TGAAAGGCAGAATGAGAAGA+TGG | 0.511267 | 8.4:-73404226 | MS.gene53319:CDS |
| AGATAATGCAAGAGTTTGAA+AGG | 0.512294 | 8.4:-73404242 | MS.gene53319:CDS |
| AAGCTTAAATCACAACTTCA+GGG | 0.512790 | 8.4:-73404838 | MS.gene53319:CDS |
| GAAAGGAGTTACTAAAGCAA+TGG | 0.516989 | 8.4:-73404304 | MS.gene53319:CDS |
| TTTAGAAGGTGATGAAGAAG+AGG | 0.518323 | 8.4:-73404163 | MS.gene53319:CDS |
| TGCTGTGAGGGTTATGGCCA+AGG | 0.523944 | 8.4:-73404899 | MS.gene53319:CDS |
| GCGAGTTCTGATAAGATCCT+TGG | 0.525743 | 8.4:+73404882 | None:intergenic |
| GTCTATTAGAGAAATTGAAA+GGG | 0.532893 | 8.4:-73405661 | MS.gene53319:CDS |
| AATTTGTTAGGGTGCTGTGA+GGG | 0.568995 | 8.4:-73404911 | MS.gene53319:intron |
| AATCAAGTTCTTGATGAGAT+CGG | 0.569717 | 8.4:-73404120 | MS.gene53319:CDS |
| GAAGATGGAACTTGTTTCTG+AGG | 0.576457 | 8.4:-73404211 | MS.gene53319:CDS |
| CAATTTGTTAGGGTGCTGTG+AGG | 0.590012 | 8.4:-73404912 | MS.gene53319:intron |
| ACTGAAATCTACTCAAGCAA+TGG | 0.611599 | 8.4:-73404337 | MS.gene53319:CDS |
| GAAATCTACTCAAGCAATGG+GGG | 0.624835 | 8.4:-73404334 | MS.gene53319:CDS |
| ATTAAGAAGAATGCCAAACA+AGG | 0.625704 | 8.4:-73405594 | MS.gene53319:CDS |
| GAAATTGAAAGGGAAAGACA+AGG | 0.633295 | 8.4:-73405651 | MS.gene53319:CDS |
| AAATTGAAAGGGAAAGACAA+GGG | 0.643400 | 8.4:-73405650 | MS.gene53319:CDS |
| CGGAATCGATATCAACTCTG+AGG | 0.660467 | 8.4:-73404100 | None:intergenic |
| TGAAATCTACTCAAGCAATG+GGG | 0.673116 | 8.4:-73404335 | MS.gene53319:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAAATTGATAATTACTTTTA+GGG | - | chr8.4:73404694-73404713 | MS.gene53319:intron | 10.0% |
| !!! | CAAAATTGATAATTACTTTT+AGG | - | chr8.4:73404693-73404712 | MS.gene53319:intron | 15.0% |
| !! | ACATTCAAATTAAACCAAAA+CGG | + | chr8.4:73404577-73404596 | None:intergenic | 20.0% |
| !! | CTAAAAACAAAAAACACTAA+TGG | - | chr8.4:73404511-73404530 | MS.gene53319:intron | 20.0% |
| !!! | AGTTTTGTTTCAATTTGTTA+GGG | - | chr8.4:73404874-73404893 | MS.gene53319:CDS | 20.0% |
| !!! | TGTTTTAGTTTCATAGTTAA+CGG | - | chr8.4:73404594-73404613 | MS.gene53319:intron | 20.0% |
| !!! | TTAGTTGTATATTTTGTTGT+AGG | - | chr8.4:73404292-73404311 | MS.gene53319:CDS | 20.0% |
| ! | AAACAAAAAACACTAATGGT+AGG | - | chr8.4:73404515-73404534 | MS.gene53319:intron | 25.0% |
| ! | AACAAAAAACACTAATGGTA+GGG | - | chr8.4:73404516-73404535 | MS.gene53319:intron | 25.0% |
| ! | AACTAAGTGCAATAGAAAAA+TGG | + | chr8.4:73404278-73404297 | None:intergenic | 25.0% |
| ! | AGTCTATTAGAGAAATTGAA+AGG | - | chr8.4:73404134-73404153 | MS.gene53319:CDS | 25.0% |
| ! | CAAAAAACACAATAATTGCA+GGG | + | chr8.4:73404335-73404354 | None:intergenic | 25.0% |
| ! | GTCTATTAGAGAAATTGAAA+GGG | - | chr8.4:73404135-73404154 | MS.gene53319:CDS | 25.0% |
| !! | AACGGTGTTAATTGTTATTA+GGG | - | chr8.4:73404612-73404631 | MS.gene53319:intron | 25.0% |
| !! | CAATAGTTTTTGAATTTGCA+AGG | + | chr8.4:73404395-73404414 | None:intergenic | 25.0% |
| !! | TAACGGTGTTAATTGTTATT+AGG | - | chr8.4:73404611-73404630 | MS.gene53319:intron | 25.0% |
| !! | TGTAAAACTTTTCAATCTGA+TGG | + | chr8.4:73404939-73404958 | None:intergenic | 25.0% |
| !!! | GAGTTTTGTTTCAATTTGTT+AGG | - | chr8.4:73404873-73404892 | MS.gene53319:CDS | 25.0% |
| !!! | TGAGAAGTTTATTGCTTTTA+AGG | - | chr8.4:73404774-73404793 | MS.gene53319:intron | 25.0% |
| !!! | TTTATTGCTTTTAAGGATTG+TGG | - | chr8.4:73404781-73404800 | MS.gene53319:intron | 25.0% |
| AAAGGAGTTACTAAAGCAAT+GGG | - | chr8.4:73405493-73405512 | MS.gene53319:intron | 30.0% | |
| AAATACCATTTGTCCTTGTT+TGG | + | chr8.4:73404218-73404237 | None:intergenic | 30.0% | |
| AAATTGAAAGGGAAAGACAA+GGG | - | chr8.4:73404146-73404165 | MS.gene53319:CDS | 30.0% | |
| AACTTATCTGCTAAAATCCT+AGG | + | chr8.4:73405067-73405086 | None:intergenic | 30.0% | |
| AAGCTTAAATCACAACTTCA+GGG | - | chr8.4:73404958-73404977 | MS.gene53319:intron | 30.0% | |
| AATCAAGTTCTTGATGAGAT+CGG | - | chr8.4:73405676-73405695 | MS.gene53319:CDS | 30.0% | |
| AGATAATGCAAGAGTTTGAA+AGG | - | chr8.4:73405554-73405573 | MS.gene53319:intron | 30.0% | |
| ATTAAGAAGAATGCCAAACA+AGG | - | chr8.4:73404202-73404221 | MS.gene53319:CDS | 30.0% | |
| CCAAAAAACACAATAATTGC+AGG | + | chr8.4:73404336-73404355 | None:intergenic | 30.0% | |
| CTCAAACAAAAGAACTTACA+CGG | + | chr8.4:73404366-73404385 | None:intergenic | 30.0% | |
| GAACAGGTATGTAATATGTT+TGG | - | chr8.4:73404831-73404850 | MS.gene53319:CDS | 30.0% | |
| GGTATGTAATATGTTTGGTT+TGG | - | chr8.4:73404836-73404855 | MS.gene53319:CDS | 30.0% | |
| GTCTGTAACATTACTTAATC+AGG | + | chr8.4:73404461-73404480 | None:intergenic | 30.0% | |
| TGCATTATCTTCTGTAAAGA+AGG | + | chr8.4:73405544-73405563 | None:intergenic | 30.0% | |
| TTTCATGCTCATTCATACTA+TGG | - | chr8.4:73405325-73405344 | MS.gene53319:intron | 30.0% | |
| ! | GTAAGTAACTGTGTTGTTAA+GGG | - | chr8.4:73404642-73404661 | MS.gene53319:intron | 30.0% |
| ! | TCGTTGTATTATATCCGTTT+TGG | - | chr8.4:73404560-73404579 | MS.gene53319:intron | 30.0% |
| ! | TGTAAGTAACTGTGTTGTTA+AGG | - | chr8.4:73404641-73404660 | MS.gene53319:intron | 30.0% |
| !!! | CCTGCAATTATTGTGTTTTT+TGG | - | chr8.4:73404333-73404352 | MS.gene53319:CDS | 30.0% |
| !!! | TGTTTTAACTACGTCATATG+CGG | - | chr8.4:73404413-73404432 | MS.gene53319:intron | 30.0% |
| AAGCAATGGGTCAAATGAAT+AGG | - | chr8.4:73405506-73405525 | MS.gene53319:intron | 35.0% | |
| ACTGAAATCTACTCAAGCAA+TGG | - | chr8.4:73405459-73405478 | MS.gene53319:intron | 35.0% | |
| CAAGCTTAAATCACAACTTC+AGG | - | chr8.4:73404957-73404976 | MS.gene53319:intron | 35.0% | |
| CAGTGTCTACAATTAATCTC+AGG | + | chr8.4:73405443-73405462 | None:intergenic | 35.0% | |
| CATTACTTAATCAGGCTACA+TGG | + | chr8.4:73404453-73404472 | None:intergenic | 35.0% | |
| CCACTAGTAATGAAAGAAGA+AGG | + | chr8.4:73404250-73404269 | None:intergenic | 35.0% | |
| CCTTCTTCTTTCATTACTAG+TGG | - | chr8.4:73404247-73404266 | MS.gene53319:CDS | 35.0% | |
| CTGAAATCTACTCAAGCAAT+GGG | - | chr8.4:73405460-73405479 | MS.gene53319:intron | 35.0% | |
| GAAAGGAGTTACTAAAGCAA+TGG | - | chr8.4:73405492-73405511 | MS.gene53319:intron | 35.0% | |
| GAAATTGAAAGGGAAAGACA+AGG | - | chr8.4:73404145-73404164 | MS.gene53319:CDS | 35.0% | |
| GCTACATGGCGTATTATATT+GGG | + | chr8.4:73404439-73404458 | None:intergenic | 35.0% | |
| TGAAATCTACTCAAGCAATG+GGG | - | chr8.4:73405461-73405480 | MS.gene53319:intron | 35.0% | |
| TGTCTACAATTAATCTCAGG+AGG | + | chr8.4:73405440-73405459 | None:intergenic | 35.0% | |
| TTTAGAAGGTGATGAAGAAG+AGG | - | chr8.4:73405633-73405652 | MS.gene53319:CDS | 35.0% | |
| ! | TGAGCATGAAAGTATACACA+AGG | + | chr8.4:73405316-73405335 | None:intergenic | 35.0% |
| !! | AGCTTTTGAGGGAGAATAAA+AGG | - | chr8.4:73404101-73404120 | MS.gene53319:CDS | 35.0% |
| !! | GAACTTGTTTCTGAGGTAAT+GGG | - | chr8.4:73405592-73405611 | MS.gene53319:CDS | 35.0% |
| !! | GCAATTGATGATGCTTTAGA+AGG | - | chr8.4:73405619-73405638 | MS.gene53319:CDS | 35.0% |
| !! | GGATTTTAGCAGATAAGTTG+AGG | - | chr8.4:73405068-73405087 | MS.gene53319:intron | 35.0% |
| !! | TAGCGTGTTGTTATTTGAAC+AGG | - | chr8.4:73404815-73404834 | MS.gene53319:intron | 35.0% |
| CTCGTTATGTATCTGCAATG+AGG | + | chr8.4:73405293-73405312 | None:intergenic | 40.0% | |
| GAAATCTACTCAAGCAATGG+GGG | - | chr8.4:73405462-73405481 | MS.gene53319:intron | 40.0% | |
| GAATGCCAAACAAGGACAAA+TGG | - | chr8.4:73404210-73404229 | MS.gene53319:CDS | 40.0% | |
| GGCTACATGGCGTATTATAT+TGG | + | chr8.4:73404440-73404459 | None:intergenic | 40.0% | |
| TGAAAGGCAGAATGAGAAGA+TGG | - | chr8.4:73405570-73405589 | MS.gene53319:intron | 40.0% | |
| TTGAAGCTTATTGTGCTCCT+AGG | - | chr8.4:73405047-73405066 | MS.gene53319:intron | 40.0% | |
| !! | AATTTGTTAGGGTGCTGTGA+GGG | - | chr8.4:73404885-73404904 | MS.gene53319:CDS | 40.0% |
| !! | GAAGATGGAACTTGTTTCTG+AGG | - | chr8.4:73405585-73405604 | MS.gene53319:intron | 40.0% |
| !! | GGAACTTGTTTCTGAGGTAA+TGG | - | chr8.4:73405591-73405610 | MS.gene53319:CDS | 40.0% |
| GACAAGTATCCGACATGTGT+GGG | - | chr8.4:73405162-73405181 | MS.gene53319:intron | 45.0% | |
| TGACAAGTATCCGACATGTG+TGG | - | chr8.4:73405161-73405180 | MS.gene53319:intron | 45.0% | |
| ! | GCGAGTTCTGATAAGATCCT+TGG | + | chr8.4:73404917-73404936 | None:intergenic | 45.0% |
| !! | CAATTTGTTAGGGTGCTGTG+AGG | - | chr8.4:73404884-73404903 | MS.gene53319:CDS | 45.0% |
| !! | GGGTGTTGCTTTGAGAATTC+AGG | - | chr8.4:73404978-73404997 | MS.gene53319:intron | 45.0% |
| !! | TTAAAAATTATAAATTTAAC+AGG | + | chr8.4:73405377-73405396 | None:intergenic | 5.0% |
| ACCGACAGAGACATGGATAC+CGG | + | chr8.4:73405269-73405288 | None:intergenic | 50.0% | |
| ATCCGACATGTGTGGGCATT+GGG | - | chr8.4:73405169-73405188 | MS.gene53319:intron | 50.0% | |
| TATCCGACATGTGTGGGCAT+TGG | - | chr8.4:73405168-73405187 | MS.gene53319:intron | 50.0% | |
| TCCGGTATCCATGTCTCTGT+CGG | - | chr8.4:73405265-73405284 | MS.gene53319:intron | 50.0% | |
| ! | CGATGTGTAAGTGTCGTGTC+CGG | - | chr8.4:73405247-73405266 | MS.gene53319:intron | 50.0% |
| !! | TTAGGGTGCTGTGAGGGTTA+TGG | - | chr8.4:73404891-73404910 | MS.gene53319:CDS | 50.0% |
| GCAATGGGGGACGCTATGAA+AGG | - | chr8.4:73405475-73405494 | MS.gene53319:intron | 55.0% | |
| TGCCCAATGCCCACACATGT+CGG | + | chr8.4:73405174-73405193 | None:intergenic | 55.0% | |
| TGCTGTGAGGGTTATGGCCA+AGG | - | chr8.4:73404897-73404916 | MS.gene53319:CDS | 55.0% | |
| !! | ATGAGGCACCGACAGAGACA+TGG | + | chr8.4:73405276-73405295 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 73404101 | 73405717 | 73404101 | ID=MS.gene53319 |
| chr8.4 | mRNA | 73404101 | 73405717 | 73404101 | ID=MS.gene53319.t1;Parent=MS.gene53319 |
| chr8.4 | exon | 73405587 | 73405717 | 73405587 | ID=MS.gene53319.t1.exon1;Parent=MS.gene53319.t1 |
| chr8.4 | CDS | 73405587 | 73405717 | 73405587 | ID=cds.MS.gene53319.t1;Parent=MS.gene53319.t1 |
| chr8.4 | exon | 73404819 | 73404923 | 73404819 | ID=MS.gene53319.t1.exon2;Parent=MS.gene53319.t1 |
| chr8.4 | CDS | 73404819 | 73404923 | 73404819 | ID=cds.MS.gene53319.t1;Parent=MS.gene53319.t1 |
| chr8.4 | exon | 73404101 | 73404361 | 73404101 | ID=MS.gene53319.t1.exon3;Parent=MS.gene53319.t1 |
| chr8.4 | CDS | 73404101 | 73404361 | 73404101 | ID=cds.MS.gene53319.t1;Parent=MS.gene53319.t1 |
| Gene Sequence |
| Protein sequence |
