AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene53872

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene53872.t1 MTR_3g062540 98.415 568 9 0 1 568 1 568 0.0 1158
MS.gene53872.t1 MTR_3g062540 98.289 526 9 0 43 568 1 526 0.0 1067
MS.gene53872.t1 MTR_5g075640 72.069 580 142 7 4 568 5 579 0.0 833
MS.gene53872.t1 MTR_5g075640 71.719 541 133 7 43 568 1 536 0.0 763
MS.gene53872.t1 MTR_5g016060 40.129 618 300 10 8 566 24 630 1.15e-144 431
MS.gene53872.t1 MTR_4g095430 38.752 609 307 10 12 568 1 595 6.89e-137 410
MS.gene53872.t1 MTR_4g022330 38.715 576 300 10 8 539 16 582 3.18e-133 402
MS.gene53872.t1 MTR_1g088885 36.174 622 317 16 7 568 20 621 9.94e-123 375
MS.gene53872.t1 MTR_7g108290 34.891 599 315 12 7 547 20 601 1.04e-115 358
MS.gene53872.t1 MTR_7g073980 37.319 552 306 10 23 539 32 578 2.30e-114 354
MS.gene53872.t1 MTR_2g018750 35.125 558 286 9 11 500 12 561 4.72e-106 331
MS.gene53872.t1 MTR_1g064360 37.849 465 268 7 18 463 20 482 1.45e-103 323
MS.gene53872.t1 MTR_8g078900 34.056 646 323 15 16 563 11 651 3.36e-103 325
MS.gene53872.t1 MTR_2g009340 32.201 618 328 7 12 541 32 646 4.01e-100 318
MS.gene53872.t1 MTR_5g016060 37.002 527 262 10 99 566 1 516 1.66e-99 311
MS.gene53872.t1 MTR_4g093850 32.353 612 327 11 13 541 33 640 4.40e-98 312
MS.gene53872.t1 MTR_8g078900 33.974 624 310 14 37 563 3 621 9.81e-98 310
MS.gene53872.t1 MTR_2g084595 33.037 563 321 9 3 510 2 563 1.35e-95 305
MS.gene53872.t1 MTR_8g078900 34.435 575 295 12 66 563 42 611 1.12e-93 299
MS.gene53872.t1 MTR_1g094850 34.702 487 273 9 16 463 17 497 1.48e-92 296
MS.gene53872.t1 MTR_5g032780 35.098 510 268 8 19 469 21 526 2.02e-88 286
MS.gene53872.t1 MTR_8g090220 31.203 532 306 9 24 508 52 570 4.50e-88 285
MS.gene53872.t1 MTR_8g090220 33.051 472 271 7 24 461 52 512 3.74e-87 279
MS.gene53872.t1 MTR_4g134210 32.391 460 288 5 19 460 21 475 2.90e-84 274
MS.gene53872.t1 MTR_5g015790 35.095 473 257 9 23 460 26 483 3.18e-84 275
MS.gene53872.t1 MTR_2g048390 35.973 442 241 7 11 410 12 453 1.67e-83 268
MS.gene53872.t1 MTR_1g014070 33.607 488 273 8 11 459 12 487 4.66e-82 269
MS.gene53872.t1 MTR_1g106430 33.264 484 269 9 21 465 25 493 9.28e-82 268
MS.gene53872.t1 MTR_8g078900 36.324 457 226 9 16 410 11 464 5.72e-81 261
MS.gene53872.t1 MTR_4g093850 33.261 463 240 8 13 410 33 491 2.60e-77 254
MS.gene53872.t1 MTR_1g082690 30.192 520 328 9 1 510 34 528 1.37e-76 253
MS.gene53872.t1 MTR_8g069330 33.468 496 270 10 27 482 29 504 7.83e-73 244
MS.gene53872.t1 MTR_2g084595 33.110 447 258 8 3 410 2 446 2.66e-72 238
MS.gene53872.t1 MTR_8g078900 37.306 386 198 6 66 410 42 424 2.75e-71 235
MS.gene53872.t1 MTR_2g027410 30.197 457 284 6 23 460 25 465 5.78e-70 236
MS.gene53872.t1 MTR_2g027410 31.296 409 246 6 71 460 1 393 9.08e-61 209
MS.gene53872.t1 MTR_4g011530 32.792 308 187 4 161 461 20 314 8.49e-50 178
MS.gene53872.t1 MTR_2g102730 34.266 286 176 3 183 466 24 299 4.69e-48 173
MS.gene53872.t1 MTR_1g105870 37.273 220 114 4 28 235 35 242 1.40e-42 154
MS.gene53872.t1 MTR_4g074190 25.388 516 276 13 66 507 3 483 1.94e-40 154
MS.gene53872.t1 MTR_4g074190 25.388 516 276 13 66 507 49 529 3.36e-40 154
MS.gene53872.t1 MTR_4g045767 25.000 524 314 13 33 508 14 506 1.98e-36 143
MS.gene53872.t1 MTR_1g035720 25.885 537 298 16 34 509 14 511 2.58e-36 142
MS.gene53872.t1 MTR_4g074190 27.456 397 209 10 66 411 49 417 4.09e-32 129
MS.gene53872.t1 MTR_2g010890 26.154 390 221 12 91 446 2 358 2.03e-25 109
MS.gene53872.t1 MTR_2g048375 33.594 128 67 5 426 537 1 126 4.23e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene53872.t1 AT2G30520 67.123 584 173 6 4 568 10 593 0.0 795
MS.gene53872.t1 AT2G30520 66.972 545 161 6 43 568 1 545 0.0 733
MS.gene53872.t1 AT2G30520 66.972 545 161 6 43 568 1 545 0.0 733
MS.gene53872.t1 AT5G67385 38.511 618 313 13 2 568 3 604 1.92e-130 394
MS.gene53872.t1 AT5G48800 38.356 584 283 11 7 539 22 579 2.51e-128 389
MS.gene53872.t1 AT1G03010 35.522 594 309 13 7 539 20 600 1.30e-119 367
MS.gene53872.t1 AT1G03010 35.813 578 298 12 23 539 8 573 2.05e-118 363
MS.gene53872.t1 AT1G03010 35.813 578 298 12 23 539 34 599 1.03e-117 362
MS.gene53872.t1 AT2G47860 36.738 558 281 12 7 509 21 561 4.90e-113 350
MS.gene53872.t1 AT5G67385 36.585 574 299 12 45 568 1 559 3.29e-112 346
MS.gene53872.t1 AT5G67385 36.585 574 299 12 45 568 1 559 3.29e-112 346
MS.gene53872.t1 AT5G67385 36.585 574 299 12 45 568 1 559 3.29e-112 346
MS.gene53872.t1 AT5G67385 36.585 574 299 12 45 568 1 559 3.29e-112 346
MS.gene53872.t1 AT1G30440 34.671 623 327 13 11 555 12 632 8.74e-112 348
MS.gene53872.t1 AT2G47860 35.284 581 281 13 7 509 21 584 6.95e-108 338
MS.gene53872.t1 AT3G08570 34.615 572 319 11 19 547 30 589 3.36e-107 335
MS.gene53872.t1 AT3G08660 34.964 552 313 14 19 545 30 560 1.85e-102 321
MS.gene53872.t1 AT5G13600 36.059 538 298 12 18 511 20 555 1.91e-101 319
MS.gene53872.t1 AT5G03250 36.454 502 266 10 9 463 10 505 5.28e-101 318
MS.gene53872.t1 AT3G08660 34.038 520 298 13 51 545 1 500 3.73e-94 298
MS.gene53872.t1 AT3G49970 34.712 556 292 16 37 568 7 515 3.17e-91 290
MS.gene53872.t1 AT3G49970 34.712 556 292 16 37 568 7 515 3.17e-91 290
MS.gene53872.t1 AT2G47860 37.443 438 226 10 7 410 21 444 3.81e-88 280
MS.gene53872.t1 AT3G50840 33.901 587 337 12 10 565 1 567 1.52e-86 279
MS.gene53872.t1 AT5G66560 31.804 654 316 14 16 547 10 655 1.60e-85 279
MS.gene53872.t1 AT2G47860 35.575 461 226 11 7 410 21 467 6.57e-83 267
MS.gene53872.t1 AT5G10250 30.853 551 322 11 18 527 44 576 1.27e-82 270
MS.gene53872.t1 AT2G14820 34.021 485 263 9 22 465 25 493 2.95e-82 270
MS.gene53872.t1 AT2G14820 34.021 485 263 9 22 465 25 493 2.95e-82 270
MS.gene53872.t1 AT5G66560 33.453 559 287 11 66 547 66 616 1.69e-81 268
MS.gene53872.t1 AT5G10250 31.608 541 316 12 18 527 44 561 1.82e-81 267
MS.gene53872.t1 AT5G66560 33.453 559 287 11 66 547 76 626 1.95e-81 268
MS.gene53872.t1 AT3G44820 30.249 562 318 11 18 510 17 573 4.46e-79 262
MS.gene53872.t1 AT2G47860 34.716 458 227 11 109 509 1 443 2.86e-78 256
MS.gene53872.t1 AT2G47860 34.716 458 227 11 109 509 1 443 2.86e-78 256
MS.gene53872.t1 AT3G50840 33.757 551 315 11 45 565 1 531 3.15e-78 256
MS.gene53872.t1 AT3G50840 33.757 551 315 11 45 565 1 531 3.15e-78 256
MS.gene53872.t1 AT1G67900 34.669 499 273 5 21 469 23 518 3.85e-77 256
MS.gene53872.t1 AT1G67900 34.669 499 273 5 21 469 23 518 3.85e-77 256
MS.gene53872.t1 AT1G67900 34.669 499 273 5 21 469 23 518 3.85e-77 256
MS.gene53872.t1 AT1G67900 34.669 499 273 5 21 469 23 518 3.85e-77 256
MS.gene53872.t1 AT1G67900 34.669 499 273 5 21 469 23 518 3.85e-77 256
MS.gene53872.t1 AT1G67900 34.669 499 273 5 21 469 23 518 3.85e-77 256
MS.gene53872.t1 AT5G67440 33.833 467 268 9 21 460 24 476 3.53e-75 249
MS.gene53872.t1 AT5G67440 33.833 467 268 9 21 460 24 476 3.53e-75 249
MS.gene53872.t1 AT4G31820 32.059 471 259 8 23 460 26 468 7.54e-75 248
MS.gene53872.t1 AT3G08660 34.963 409 229 10 19 411 30 417 5.34e-74 242
MS.gene53872.t1 AT5G47800 30.217 460 295 6 19 458 21 474 1.69e-71 239
MS.gene53872.t1 AT5G47800 30.217 460 295 6 19 458 21 474 1.69e-71 239
MS.gene53872.t1 AT5G47800 30.217 460 295 6 19 458 21 474 1.69e-71 239
MS.gene53872.t1 AT4G37590 31.417 487 270 10 21 475 24 478 1.24e-70 238
MS.gene53872.t1 AT5G47800 30.482 456 291 6 23 458 138 587 2.36e-70 239
MS.gene53872.t1 AT2G23050 31.924 473 258 9 21 468 24 457 1.51e-69 232
MS.gene53872.t1 AT5G03250 34.085 399 213 8 109 463 1 393 2.28e-69 231
MS.gene53872.t1 AT5G67440 32.962 449 260 9 39 460 5 439 3.86e-68 230
MS.gene53872.t1 AT4G31820 30.926 443 245 8 51 460 1 415 5.57e-65 221
MS.gene53872.t1 AT5G66560 32.359 479 232 10 16 410 10 480 1.58e-64 219
MS.gene53872.t1 AT3G26490 29.811 530 281 12 21 487 23 524 1.42e-61 214
MS.gene53872.t1 AT3G26490 29.811 530 281 12 21 487 23 524 1.42e-61 214
MS.gene53872.t1 AT3G26490 29.811 530 281 12 21 487 23 524 1.42e-61 214
MS.gene53872.t1 AT1G52770 35.664 286 168 4 183 461 28 304 6.31e-50 179
MS.gene53872.t1 AT5G48130 28.131 487 270 14 18 467 33 476 5.71e-49 180
MS.gene53872.t1 AT3G15570 35.616 292 160 5 185 461 31 309 8.20e-49 176
MS.gene53872.t1 AT3G26490 29.677 465 244 12 21 420 23 469 7.67e-47 171
MS.gene53872.t1 AT5G64330 35.409 257 125 3 14 229 42 298 3.00e-46 172
MS.gene53872.t1 AT5G64330 34.363 259 139 2 225 461 357 606 1.24e-39 154
MS.gene53872.t1 AT5G64330 35.409 257 125 3 14 229 42 298 4.40e-46 173
MS.gene53872.t1 AT5G64330 29.178 377 210 5 225 553 357 724 1.89e-40 157
MS.gene53872.t1 AT3G19850 26.115 471 277 7 37 459 17 464 7.10e-46 170
MS.gene53872.t1 AT2G23050 29.737 380 208 8 109 468 1 341 7.05e-43 157
MS.gene53872.t1 AT1G50280 27.637 474 265 12 33 454 13 460 5.06e-41 155
MS.gene53872.t1 AT5G64330 34.363 259 139 2 225 461 298 547 7.62e-40 154
MS.gene53872.t1 AT5G64330 35.043 234 111 3 37 229 6 239 1.90e-37 147
MS.gene53872.t1 AT1G50280 28.211 436 240 11 66 454 18 427 9.99e-40 151
MS.gene53872.t1 AT3G03510 24.894 470 305 10 74 537 4 431 6.82e-34 134
MS.gene53872.t1 AT5G17580 25.000 460 300 9 26 462 22 459 5.33e-31 127
MS.gene53872.t1 AT5G17580 25.000 460 300 9 26 462 7 444 7.41e-31 127
MS.gene53872.t1 AT3G22104 25.166 453 268 14 26 445 6 420 3.44e-27 115
MS.gene53872.t1 AT3G22104 24.474 380 219 11 96 445 4 345 4.36e-19 90.5
MS.gene53872.t1 AT3G22104 24.474 380 219 11 96 445 4 345 4.36e-19 90.5
MS.gene53872.t1 AT3G22104 24.474 380 219 11 96 445 4 345 4.36e-19 90.5
MS.gene53872.t1 AT3G49900 32.934 167 89 7 9 163 49 204 4.86e-13 72.0
MS.gene53872.t1 AT3G49900 32.934 167 89 7 9 163 49 204 5.22e-13 72.0

Find 114 sgRNAs with CRISPR-Local

Find 153 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCACAGGCCCATCTTCTAAA+TGG 0.198824 3.1:+52611401 None:intergenic
CGCAGCGTTTCACGATGTTT+AGG 0.335013 3.1:+52612687 None:intergenic
GAACTCAACGACGTCGTTCC+AGG 0.336876 3.1:+52611502 None:intergenic
CACAATACTGATCAGTCATT+TGG 0.343048 3.1:+52612825 None:intergenic
CTACATCAGAAAACTAATAA+TGG 0.343477 3.1:-52612999 MS.gene53872:CDS
CTTTCGATTTCATCTAGCTT+TGG 0.349959 3.1:+52611811 None:intergenic
TATCGGAAAAGCAGCTTTCT+CGG 0.352377 3.1:+52612358 None:intergenic
GATTGATAATTGATTCTAGA+AGG 0.367450 3.1:+52612390 None:intergenic
GCTGCTTTCTCAAAGATTCC+AGG 0.370649 3.1:+52612923 None:intergenic
ATGGCAAGAGAGAGTCTATT+AGG 0.377719 3.1:+52613454 None:intergenic
TGATGACTGGATTCCATTAC+CGG 0.378656 3.1:+52612451 None:intergenic
ATTCGCCTCGCTGCAAGCCT+TGG 0.399473 3.1:+52612649 None:intergenic
ATCGTAAAACTGCGACATTA+TGG 0.413061 3.1:+52612864 None:intergenic
GTTCGAAACCACTCCGGTAA+TGG 0.418948 3.1:-52612464 MS.gene53872:CDS
CTAGCCGTTCACCCGCGAAT+TGG 0.420539 3.1:-52612622 MS.gene53872:CDS
GCCGTTCACCCGCGAATTGG+TGG 0.421868 3.1:-52612619 MS.gene53872:CDS
TTTAGCGACGGATGTGTCAT+TGG 0.423214 3.1:-52611608 MS.gene53872:CDS
TATTGTGACAACAACCTTGC+TGG 0.426351 3.1:-52612809 MS.gene53872:CDS
TGAGATTCGTTTCTCGAGTT+CGG 0.432245 3.1:+52612274 None:intergenic
CATCTCAGTATTCGTTGAAA+AGG 0.446389 3.1:-52612156 MS.gene53872:CDS
TCTGACATACCAGGAGGTCC+TGG 0.459917 3.1:-52612941 MS.gene53872:CDS
AGTGGTTTCGAACTAGCTCC+TGG 0.465595 3.1:+52612474 None:intergenic
CACAGGCCCATCTTCTAAAT+GGG 0.466417 3.1:+52611402 None:intergenic
AGCGCGGCTGCAATGGTTGG+AGG 0.466445 3.1:+52612521 None:intergenic
TCAAAGATTCCAGGACCTCC+TGG 0.468786 3.1:+52612932 None:intergenic
GGAATCCAGTCATCAGATTA+CGG 0.472057 3.1:-52612443 MS.gene53872:CDS
AGGCAGCAGAGGAAGTTTAT+CGG 0.479909 3.1:+52612341 None:intergenic
GATGTTTAGGTCATCGGCAT+AGG 0.480481 3.1:+52612700 None:intergenic
AATAGAGAACCTTCTTCTCC+TGG 0.481171 3.1:+52611366 None:intergenic
TGCACGCATCACAGAATAAA+CGG 0.482806 3.1:-52611735 MS.gene53872:CDS
CGTCAACGGTTACGTGTTCA+AGG 0.483329 3.1:+52612246 None:intergenic
TCTCTTGCCATGGAGAGAAC+TGG 0.495880 3.1:-52613442 MS.gene53872:CDS
GATACGGCCCATTTAGAAGA+TGG 0.496730 3.1:-52611409 MS.gene53872:CDS
TGACTAAGCCCAGGAGAAGA+AGG 0.496892 3.1:-52611375 MS.gene53872:CDS
TTCCTCAGTCCACCAATTCG+CGG 0.498945 3.1:+52612610 None:intergenic
GAAGATGAAGATGTACATTA+CGG 0.500998 3.1:-52611551 MS.gene53872:CDS
CGCAACGGAGGAGGCAGCAG+AGG 0.501740 3.1:+52612330 None:intergenic
CTCGCTGAAATCGCCGCGTA+CGG 0.501994 3.1:-52612044 MS.gene53872:CDS
ATCAGCGCGGCTGCAATGGT+TGG 0.510359 3.1:+52612518 None:intergenic
GGAGCCAGTGAGAGTGAAGA+AGG 0.510417 3.1:+52612757 None:intergenic
AATGATTCTTTGAGTTCTCC+CGG 0.510540 3.1:+52612110 None:intergenic
CGTTGAAGCTGCAAGCGCCA+AGG 0.511556 3.1:-52612666 MS.gene53872:CDS
GCTTCGTAGGATAGTTTCAA+TGG 0.512007 3.1:+52611763 None:intergenic
GGTGGATTGCGCAACGGAGG+AGG 0.517135 3.1:+52612321 None:intergenic
AAAGCAGCTAAATTCTGTTA+CGG 0.532489 3.1:-52612908 MS.gene53872:CDS
TCCTCAGTCCACCAATTCGC+GGG 0.533006 3.1:+52612611 None:intergenic
AGACGGTGGAGGCACGGAGG+TGG 0.534358 3.1:+52612303 None:intergenic
GGTCATCGGCATAGGGAAGA+AGG 0.536469 3.1:+52612708 None:intergenic
TCCACCAATTCGCGGGTGAA+CGG 0.537630 3.1:+52612618 None:intergenic
CAATTCGCGGGTGAACGGCT+AGG 0.539738 3.1:+52612623 None:intergenic
GAAAAGCAGCTTTCTCGGAC+GGG 0.540408 3.1:+52612363 None:intergenic
AATTCGCGGGTGAACGGCTA+GGG 0.540486 3.1:+52612624 None:intergenic
TTTCACGATGTTTAGGTCAT+CGG 0.541398 3.1:+52612694 None:intergenic
GTCGCCTTCTTCACTCTCAC+TGG 0.542590 3.1:-52612761 MS.gene53872:CDS
TGTAATCAGCGCGGCTGCAA+TGG 0.543689 3.1:+52612514 None:intergenic
CGAATGCGACGGAGCTGCGA+CGG 0.545270 3.1:-52611641 MS.gene53872:CDS
CAGCAGAAGAACGTTACGCC+TGG 0.545309 3.1:-52611520 MS.gene53872:CDS
ACTGATGTTATAGTTGAAGT+AGG 0.549573 3.1:-52613288 MS.gene53872:CDS
ACTTCAACTATAACATCAGT+TGG 0.549705 3.1:+52613291 None:intergenic
GTGACAACAACCTTGCTGGC+AGG 0.550266 3.1:-52612805 MS.gene53872:CDS
AATCACCGTAATCTGATGAC+TGG 0.551318 3.1:+52612438 None:intergenic
ATACTCAGAGCGATCACTCC+AGG 0.554139 3.1:-52612492 MS.gene53872:CDS
CGGCGTCAACTTCGAGATAA+CGG 0.554901 3.1:-52612888 MS.gene53872:CDS
ATAGAGAACCTTCTTCTCCT+GGG 0.555992 3.1:+52611367 None:intergenic
GAAGAGTTTCTCGCCGTCGT+AGG 0.556907 3.1:+52612202 None:intergenic
ATGTTTAGGTCATCGGCATA+GGG 0.559679 3.1:+52612701 None:intergenic
ATGATTGCAGTTCATGTTGG+TGG 0.563694 3.1:-52613032 MS.gene53872:intron
CGCCGTTGATATCTATCTCA+AGG 0.566265 3.1:-52611940 MS.gene53872:intron
TAATAGACTCTCTCTTGCCA+TGG 0.568176 3.1:-52613452 MS.gene53872:CDS
ATACGGCCCATTTAGAAGAT+GGG 0.571365 3.1:-52611408 MS.gene53872:CDS
ATAGATCTCTCTGACATACC+AGG 0.571727 3.1:-52612950 MS.gene53872:CDS
ATTCGTCGGTCCTGCCAGCA+AGG 0.576526 3.1:+52612795 None:intergenic
GCGTGTAAAACAATCTGCAC+TGG 0.578874 3.1:+52611709 None:intergenic
CCATTGGCCAGTTCTCTCCA+TGG 0.580279 3.1:+52613435 None:intergenic
GAAGGTGGTGGCAAGACTTG+AGG 0.587288 3.1:+52612726 None:intergenic
CGGCATAGGGAAGAAGGTGG+TGG 0.587751 3.1:+52612714 None:intergenic
TCAGACGACGGCGAATGCGA+CGG 0.587952 3.1:-52611652 MS.gene53872:CDS
CATCACGAGGAGGTCGTCAA+CGG 0.588515 3.1:+52612232 None:intergenic
CTGTGGATTTGACTAAGCCC+AGG 0.590340 3.1:-52611384 MS.gene53872:CDS
GTCATTGGTGAGAGAGAATG+AGG 0.591308 3.1:-52611593 MS.gene53872:CDS
CTGGGCTTAGTCAAATCCAC+AGG 0.593734 3.1:+52611385 None:intergenic
CTCAGCATCTCAAAGTTCAA+CGG 0.595833 3.1:-52612017 MS.gene53872:CDS
GGAAAAGCAGCTTTCTCGGA+CGG 0.597212 3.1:+52612362 None:intergenic
CACGGAGGTGGATTGCGCAA+CGG 0.601317 3.1:+52612315 None:intergenic
ACTACGATCAGTTGAAGTTG+AGG 0.608135 3.1:-52611681 MS.gene53872:CDS
CGCCACACGTAGCATAGCGA+CGG 0.609350 3.1:+52612085 None:intergenic
GACTTTAGAGAGAAATTCGT+CGG 0.610883 3.1:+52612781 None:intergenic
AATATGATTGCAGTTCATGT+TGG 0.613034 3.1:-52613035 MS.gene53872:intron
CTCCGTCGCTATGCTACGTG+TGG 0.615178 3.1:-52612087 MS.gene53872:CDS
TACCTTGAGATAGATATCAA+CGG 0.618572 3.1:+52611938 None:intergenic
TTCAGCGAGGTACATGTCGA+CGG 0.620480 3.1:+52612058 None:intergenic
CCATGGAGAGAACTGGCCAA+TGG 0.634800 3.1:-52613435 MS.gene53872:intron
ACTGGATTCCATTACCGGAG+TGG 0.637609 3.1:+52612456 None:intergenic
GGAGGTGGATTGCGCAACGG+AGG 0.644352 3.1:+52612318 None:intergenic
TGCGTGCACACGCGCTTCGT+AGG 0.645726 3.1:+52611750 None:intergenic
GCAAGACTTGAGGACTGCAA+CGG 0.653304 3.1:+52612736 None:intergenic
GAGCTAGTTCGAAACCACTC+CGG 0.654700 3.1:-52612470 MS.gene53872:CDS
GGAGAGAAATCGTTTAGCGA+CGG 0.657325 3.1:-52611620 MS.gene53872:CDS
GATCAGTTGAAGTTGAGGAG+TGG 0.665109 3.1:-52611676 MS.gene53872:CDS
CGTAGGAGAATGACATCACG+AGG 0.666273 3.1:+52612219 None:intergenic
GATGCTGAGTTCACCGTACG+CGG 0.666328 3.1:+52612031 None:intergenic
CCATTTAGAAGATGGGCCTG+TGG 0.683204 3.1:-52611401 MS.gene53872:CDS
GTGATGTCATTCTCCTACGA+CGG 0.684941 3.1:-52612215 MS.gene53872:CDS
AAGCGCCAAGGCTTGCAGCG+AGG 0.692127 3.1:-52612654 MS.gene53872:CDS
TTGAGGAGTGGATCAGACGA+CGG 0.697497 3.1:-52611664 MS.gene53872:CDS
TGCAGACGGTGGAGGCACGG+AGG 0.699358 3.1:+52612300 None:intergenic
ACCCGCGAATTGGTGGACTG+AGG 0.710857 3.1:-52612612 MS.gene53872:CDS
CATCGGCATAGGGAAGAAGG+TGG 0.713733 3.1:+52612711 None:intergenic
ATCGCATGTATGAAACAGCG+CGG 0.732891 3.1:-52612551 MS.gene53872:CDS
CTCTGAGTATGTAATCAGCG+CGG 0.746517 3.1:+52612505 None:intergenic
AGGAGAATGACATCACGAGG+AGG 0.750611 3.1:+52612222 None:intergenic
GATCTCTCTGACATACCAGG+AGG 0.759330 3.1:-52612947 MS.gene53872:CDS
TGAGATAGATATCAACGGCG+CGG 0.763641 3.1:+52611943 None:intergenic
CGTACGCGGCGATTTCAGCG+AGG 0.778421 3.1:+52612045 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAGAATACCTTAACAAATT+TGG - chr3.1:52612916-52612935 MS.gene53872:CDS 20.0%
!! AATATACAAACTCGTTTAAA+CGG + chr3.1:52612981-52613000 None:intergenic 20.0%
!! ATGAACAATATAATTCACAT+GGG + chr3.1:52611604-52611623 None:intergenic 20.0%
!! TATGAACAATATAATTCACA+TGG + chr3.1:52611605-52611624 None:intergenic 20.0%
!! TCAAAGTACAAAATAGAAAA+TGG + chr3.1:52611484-52611503 None:intergenic 20.0%
!!! ATTCAAATCCAAATTTGTTA+AGG + chr3.1:52612927-52612946 None:intergenic 20.0%
! ATAAATGTGATTACATGTGT+CGG - chr3.1:52611700-52611719 MS.gene53872:CDS 25.0%
! CTACATCAGAAAACTAATAA+TGG - chr3.1:52611827-52611846 MS.gene53872:intron 25.0%
! GTAATCACATTTATTGATGT+TGG + chr3.1:52611694-52611713 None:intergenic 25.0%
! GTACTTTGATTATTATTCCA+GGG - chr3.1:52611495-52611514 MS.gene53872:CDS 25.0%
! TGTACTTTGATTATTATTCC+AGG - chr3.1:52611494-52611513 MS.gene53872:CDS 25.0%
!! GATTGATAATTGATTCTAGA+AGG + chr3.1:52612439-52612458 None:intergenic 25.0%
!!! CGAGTTTGTATATTTTTTTC+AGG - chr3.1:52612987-52613006 MS.gene53872:CDS 25.0%
AAAGCAGCTAAATTCTGTTA+CGG - chr3.1:52611918-52611937 MS.gene53872:intron 30.0%
AATATGATTGCAGTTCATGT+TGG - chr3.1:52611791-52611810 MS.gene53872:CDS 30.0%
ACTGATGTTATAGTTGAAGT+AGG - chr3.1:52611538-52611557 MS.gene53872:CDS 30.0%
ACTTCAACTATAACATCAGT+TGG + chr3.1:52611538-52611557 None:intergenic 30.0%
GAAGATGAAGATGTACATTA+CGG - chr3.1:52613275-52613294 MS.gene53872:CDS 30.0%
TACCTTGAGATAGATATCAA+CGG + chr3.1:52612891-52612910 None:intergenic 30.0%
TGGGAAAGTTGTTCAAAAAT+GGG - chr3.1:52613388-52613407 MS.gene53872:intron 30.0%
! AGAAAAAGTATGCAGCATAA+TGG - chr3.1:52613038-52613057 MS.gene53872:intron 30.0%
!!! AATTCACATGGGTCATTTTT+TGG + chr3.1:52611593-52611612 None:intergenic 30.0%
ATCGTAAAACTGCGACATTA+TGG + chr3.1:52611965-52611984 None:intergenic 35.0%
CACAATACTGATCAGTCATT+TGG + chr3.1:52612004-52612023 None:intergenic 35.0%
CATCTCAGTATTCGTTGAAA+AGG - chr3.1:52612670-52612689 MS.gene53872:CDS 35.0%
CTGGGAAAGTTGTTCAAAAA+TGG - chr3.1:52613387-52613406 MS.gene53872:intron 35.0%
GGGAAAGTTGTTCAAAAATG+GGG - chr3.1:52613389-52613408 MS.gene53872:intron 35.0%
TGCATGCATGGATTTGAAAA+TGG + chr3.1:52611445-52611464 None:intergenic 35.0%
TTTAGTGTGTTGTGTACCAT+TGG + chr3.1:52611410-52611429 None:intergenic 35.0%
TTTCACGATGTTTAGGTCAT+CGG + chr3.1:52612135-52612154 None:intergenic 35.0%
! AATGATTCTTTGAGTTCTCC+CGG + chr3.1:52612719-52612738 None:intergenic 35.0%
! CTTTCGATTTCATCTAGCTT+TGG + chr3.1:52613018-52613037 None:intergenic 35.0%
! GACTTTAGAGAGAAATTCGT+CGG + chr3.1:52612048-52612067 None:intergenic 35.0%
!!! ATTTTTTGGATACCTTGTGG+AGG + chr3.1:52611579-52611598 None:intergenic 35.0%
!!! GTCATTTTTTGGATACCTTG+TGG + chr3.1:52611582-52611601 None:intergenic 35.0%
AACTTCTTGAGAGAAAACCC+TGG + chr3.1:52611515-52611534 None:intergenic 40.0%
AATAGAGAACCTTCTTCTCC+TGG + chr3.1:52613463-52613482 None:intergenic 40.0%
AATCACCGTAATCTGATGAC+TGG + chr3.1:52612391-52612410 None:intergenic 40.0%
ACTACGATCAGTTGAAGTTG+AGG - chr3.1:52613145-52613164 MS.gene53872:intron 40.0%
ATACGGCCCATTTAGAAGAT+GGG - chr3.1:52613418-52613437 MS.gene53872:intron 40.0%
ATAGAGAACCTTCTTCTCCT+GGG + chr3.1:52613462-52613481 None:intergenic 40.0%
ATAGATCTCTCTGACATACC+AGG - chr3.1:52611876-52611895 MS.gene53872:intron 40.0%
ATGATTGCAGTTCATGTTGG+TGG - chr3.1:52611794-52611813 MS.gene53872:CDS 40.0%
ATGGCAAGAGAGAGTCTATT+AGG + chr3.1:52611375-52611394 None:intergenic 40.0%
ATGTTTAGGTCATCGGCATA+GGG + chr3.1:52612128-52612147 None:intergenic 40.0%
CAAAAATGGGGCTAAGGATA+CGG - chr3.1:52613401-52613420 MS.gene53872:intron 40.0%
CTCAGCATCTCAAAGTTCAA+CGG - chr3.1:52612809-52612828 MS.gene53872:CDS 40.0%
GCTTCGTAGGATAGTTTCAA+TGG + chr3.1:52613066-52613085 None:intergenic 40.0%
GGAATCCAGTCATCAGATTA+CGG - chr3.1:52612383-52612402 MS.gene53872:CDS 40.0%
GTTGTTCAAAAATGGGGCTA+AGG - chr3.1:52613395-52613414 MS.gene53872:intron 40.0%
TAATAGACTCTCTCTTGCCA+TGG - chr3.1:52611374-52611393 MS.gene53872:CDS 40.0%
TATCGGAAAAGCAGCTTTCT+CGG + chr3.1:52612471-52612490 None:intergenic 40.0%
TATTGTGACAACAACCTTGC+TGG - chr3.1:52612017-52612036 MS.gene53872:CDS 40.0%
TCTTCCGTGTCGAAAAAACT+GGG - chr3.1:52613369-52613388 MS.gene53872:intron 40.0%
TGAGATTCGTTTCTCGAGTT+CGG + chr3.1:52612555-52612574 None:intergenic 40.0%
TGATGACTGGATTCCATTAC+CGG + chr3.1:52612378-52612397 None:intergenic 40.0%
TGCACGCATCACAGAATAAA+CGG - chr3.1:52613091-52613110 MS.gene53872:intron 40.0%
TTCTTCCGTGTCGAAAAAAC+TGG - chr3.1:52613368-52613387 MS.gene53872:intron 40.0%
!!! CTTTCCCAGTTTTTTCGACA+CGG + chr3.1:52613376-52613395 None:intergenic 40.0%
AGGCAGCAGAGGAAGTTTAT+CGG + chr3.1:52612488-52612507 None:intergenic 45.0%
ATCGCATGTATGAAACAGCG+CGG - chr3.1:52612275-52612294 MS.gene53872:CDS 45.0%
CACAGGCCCATCTTCTAAAT+GGG + chr3.1:52613427-52613446 None:intergenic 45.0%
CGCCGTTGATATCTATCTCA+AGG - chr3.1:52612886-52612905 MS.gene53872:CDS 45.0%
CTCTGAGTATGTAATCAGCG+CGG + chr3.1:52612324-52612343 None:intergenic 45.0%
GATACGGCCCATTTAGAAGA+TGG - chr3.1:52613417-52613436 MS.gene53872:intron 45.0%
GATCAGTTGAAGTTGAGGAG+TGG - chr3.1:52613150-52613169 MS.gene53872:intron 45.0%
GATGTTTAGGTCATCGGCAT+AGG + chr3.1:52612129-52612148 None:intergenic 45.0%
GCGTGTAAAACAATCTGCAC+TGG + chr3.1:52613120-52613139 None:intergenic 45.0%
GGAGAGAAATCGTTTAGCGA+CGG - chr3.1:52613206-52613225 MS.gene53872:intron 45.0%
GTCATTGGTGAGAGAGAATG+AGG - chr3.1:52613233-52613252 MS.gene53872:intron 45.0%
GTGATGTCATTCTCCTACGA+CGG - chr3.1:52612611-52612630 MS.gene53872:CDS 45.0%
TGAGATAGATATCAACGGCG+CGG + chr3.1:52612886-52612905 None:intergenic 45.0%
TTTAGCGACGGATGTGTCAT+TGG - chr3.1:52613218-52613237 MS.gene53872:intron 45.0%
! AAACGCTGCAGTTTTCACAC+CGG - chr3.1:52612697-52612716 MS.gene53872:CDS 45.0%
! GCTGCTTTCTCAAAGATTCC+AGG + chr3.1:52611906-52611925 None:intergenic 45.0%
! TCGATTTTACGCGCGTGTTT+AGG + chr3.1:52612851-52612870 None:intergenic 45.0%
ACTGGATTCCATTACCGGAG+TGG + chr3.1:52612373-52612392 None:intergenic 50.0%
AGGAGAATGACATCACGAGG+AGG + chr3.1:52612607-52612626 None:intergenic 50.0%
AGTGGTTTCGAACTAGCTCC+TGG + chr3.1:52612355-52612374 None:intergenic 50.0%
ATACTCAGAGCGATCACTCC+AGG - chr3.1:52612334-52612353 MS.gene53872:CDS 50.0%
CCACAGGCCCATCTTCTAAA+TGG + chr3.1:52613428-52613447 None:intergenic 50.0%
CCATTTAGAAGATGGGCCTG+TGG - chr3.1:52613425-52613444 MS.gene53872:intron 50.0%
CGCAGCGTTTCACGATGTTT+AGG + chr3.1:52612142-52612161 None:intergenic 50.0%
CGGCGTCAACTTCGAGATAA+CGG - chr3.1:52611938-52611957 MS.gene53872:intron 50.0%
CGTAGGAGAATGACATCACG+AGG + chr3.1:52612610-52612629 None:intergenic 50.0%
CGTCAACGGTTACGTGTTCA+AGG + chr3.1:52612583-52612602 None:intergenic 50.0%
CTGGGCTTAGTCAAATCCAC+AGG + chr3.1:52613444-52613463 None:intergenic 50.0%
CTGTGGATTTGACTAAGCCC+AGG - chr3.1:52613442-52613461 MS.gene53872:CDS 50.0%
GAAAAGCAGCTTTCTCGGAC+GGG + chr3.1:52612466-52612485 None:intergenic 50.0%
GAGCTAGTTCGAAACCACTC+CGG - chr3.1:52612356-52612375 MS.gene53872:CDS 50.0%
GATCTCTCTGACATACCAGG+AGG - chr3.1:52611879-52611898 MS.gene53872:intron 50.0%
GCAAGACTTGAGGACTGCAA+CGG + chr3.1:52612093-52612112 None:intergenic 50.0%
GCGTGTGTATGATGCATGCA+TGG + chr3.1:52611457-52611476 None:intergenic 50.0%
GGAAAAGCAGCTTTCTCGGA+CGG + chr3.1:52612467-52612486 None:intergenic 50.0%
GTTCGAAACCACTCCGGTAA+TGG - chr3.1:52612362-52612381 MS.gene53872:CDS 50.0%
TCAAAGATTCCAGGACCTCC+TGG + chr3.1:52611897-52611916 None:intergenic 50.0%
TCTCTTGCCATGGAGAGAAC+TGG - chr3.1:52611384-52611403 MS.gene53872:CDS 50.0%
TGACTAAGCCCAGGAGAAGA+AGG - chr3.1:52613451-52613470 MS.gene53872:CDS 50.0%
TTCAGCGAGGTACATGTCGA+CGG + chr3.1:52612771-52612790 None:intergenic 50.0%
TTCCTCAGTCCACCAATTCG+CGG + chr3.1:52612219-52612238 None:intergenic 50.0%
TTGAGGAGTGGATCAGACGA+CGG - chr3.1:52613162-52613181 MS.gene53872:intron 50.0%
! AACGCTGCAGTTTTCACACC+GGG - chr3.1:52612698-52612717 MS.gene53872:CDS 50.0%
! CTCGAGTTCGGTTTTGCAGA+CGG + chr3.1:52612543-52612562 None:intergenic 50.0%
!! AGCTCGTTTTTGCCTCCACA+AGG - chr3.1:52611564-52611583 MS.gene53872:CDS 50.0%
AATTCGCGGGTGAACGGCTA+GGG + chr3.1:52612205-52612224 None:intergenic 55.0%
CAGCAGAAGAACGTTACGCC+TGG - chr3.1:52613306-52613325 MS.gene53872:CDS 55.0%
CATCACGAGGAGGTCGTCAA+CGG + chr3.1:52612597-52612616 None:intergenic 55.0%
CATCGGCATAGGGAAGAAGG+TGG + chr3.1:52612118-52612137 None:intergenic 55.0%
CCAGACAAGCCTTCGATCAC+AGG + chr3.1:52611651-52611670 None:intergenic 55.0%
CCATGGAGAGAACTGGCCAA+TGG - chr3.1:52611391-52611410 MS.gene53872:CDS 55.0%
CCATTGGCCAGTTCTCTCCA+TGG + chr3.1:52611394-52611413 None:intergenic 55.0%
CCTGTGATCGAAGGCTTGTC+TGG - chr3.1:52611648-52611667 MS.gene53872:CDS 55.0%
GAACTCAACGACGTCGTTCC+AGG + chr3.1:52613327-52613346 None:intergenic 55.0%
GAAGAGTTTCTCGCCGTCGT+AGG + chr3.1:52612627-52612646 None:intergenic 55.0%
GACAAGCCTTCGATCACAGG+TGG + chr3.1:52611648-52611667 None:intergenic 55.0%
GATGCTGAGTTCACCGTACG+CGG + chr3.1:52612798-52612817 None:intergenic 55.0%
GGAGCCAGTGAGAGTGAAGA+AGG + chr3.1:52612072-52612091 None:intergenic 55.0%
GGTCATCGGCATAGGGAAGA+AGG + chr3.1:52612121-52612140 None:intergenic 55.0%
GTCGCCTTCTTCACTCTCAC+TGG - chr3.1:52612065-52612084 MS.gene53872:CDS 55.0%
GTGACAACAACCTTGCTGGC+AGG - chr3.1:52612021-52612040 MS.gene53872:CDS 55.0%
TCCACCAATTCGCGGGTGAA+CGG + chr3.1:52612211-52612230 None:intergenic 55.0%
TCCTCAGTCCACCAATTCGC+GGG + chr3.1:52612218-52612237 None:intergenic 55.0%
TCTGACATACCAGGAGGTCC+TGG - chr3.1:52611885-52611904 MS.gene53872:intron 55.0%
TGTAATCAGCGCGGCTGCAA+TGG + chr3.1:52612315-52612334 None:intergenic 55.0%
! GAAGGTGGTGGCAAGACTTG+AGG + chr3.1:52612103-52612122 None:intergenic 55.0%
! GAGTTCGGTTTTGCAGACGG+TGG + chr3.1:52612540-52612559 None:intergenic 55.0%
! TTCGGTTTTGCAGACGGTGG+AGG + chr3.1:52612537-52612556 None:intergenic 55.0%
! TTTTGCAGACGGTGGAGGCA+CGG + chr3.1:52612532-52612551 None:intergenic 55.0%
ATCAGCGCGGCTGCAATGGT+TGG + chr3.1:52612311-52612330 None:intergenic 60.0%
ATTCGCCTCGCTGCAAGCCT+TGG + chr3.1:52612180-52612199 None:intergenic 60.0%
ATTCGTCGGTCCTGCCAGCA+AGG + chr3.1:52612034-52612053 None:intergenic 60.0%
CAATTCGCGGGTGAACGGCT+AGG + chr3.1:52612206-52612225 None:intergenic 60.0%
CGCCACACGTAGCATAGCGA+CGG + chr3.1:52612744-52612763 None:intergenic 60.0%
CGGCATAGGGAAGAAGGTGG+TGG + chr3.1:52612115-52612134 None:intergenic 60.0%
CGTTGAAGCTGCAAGCGCCA+AGG - chr3.1:52612160-52612179 MS.gene53872:CDS 60.0%
CTAGCCGTTCACCCGCGAAT+TGG - chr3.1:52612204-52612223 MS.gene53872:CDS 60.0%
CTCCGTCGCTATGCTACGTG+TGG - chr3.1:52612739-52612758 MS.gene53872:CDS 60.0%
CTCGCTGAAATCGCCGCGTA+CGG - chr3.1:52612782-52612801 MS.gene53872:CDS 60.0%
CTTGCGCCACCTGTGATCGA+AGG - chr3.1:52611639-52611658 MS.gene53872:CDS 60.0%
TCAGACGACGGCGAATGCGA+CGG - chr3.1:52613174-52613193 MS.gene53872:intron 60.0%
!! ACCCGCGAATTGGTGGACTG+AGG - chr3.1:52612214-52612233 MS.gene53872:CDS 60.0%
!! CACGGAGGTGGATTGCGCAA+CGG + chr3.1:52612514-52612533 None:intergenic 60.0%
AAGCGCCAAGGCTTGCAGCG+AGG - chr3.1:52612172-52612191 MS.gene53872:CDS 65.0%
AGCGCGGCTGCAATGGTTGG+AGG + chr3.1:52612308-52612327 None:intergenic 65.0%
CGAATGCGACGGAGCTGCGA+CGG - chr3.1:52613185-52613204 MS.gene53872:intron 65.0%
CGTACGCGGCGATTTCAGCG+AGG + chr3.1:52612784-52612803 None:intergenic 65.0%
GCCGTTCACCCGCGAATTGG+TGG - chr3.1:52612207-52612226 MS.gene53872:CDS 65.0%
TGCGTGCACACGCGCTTCGT+AGG + chr3.1:52613079-52613098 None:intergenic 65.0%
!! GGAGGTGGATTGCGCAACGG+AGG + chr3.1:52612511-52612530 None:intergenic 65.0%
!! GGTGGATTGCGCAACGGAGG+AGG + chr3.1:52612508-52612527 None:intergenic 65.0%
CGCAACGGAGGAGGCAGCAG+AGG + chr3.1:52612499-52612518 None:intergenic 70.0%
! AGACGGTGGAGGCACGGAGG+TGG + chr3.1:52612526-52612545 None:intergenic 70.0%
! TGCAGACGGTGGAGGCACGG+AGG + chr3.1:52612529-52612548 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr3.1 gene 52611360 52613488 52611360 ID=MS.gene53872
chr3.1 mRNA 52611360 52613488 52611360 ID=MS.gene53872.t1;Parent=MS.gene53872
chr3.1 exon 52613436 52613488 52613436 ID=MS.gene53872.t1.exon1;Parent=MS.gene53872.t1
chr3.1 CDS 52613436 52613488 52613436 ID=cds.MS.gene53872.t1;Parent=MS.gene53872.t1
chr3.1 exon 52613263 52613332 52613263 ID=MS.gene53872.t1.exon2;Parent=MS.gene53872.t1
chr3.1 CDS 52613263 52613332 52613263 ID=cds.MS.gene53872.t1;Parent=MS.gene53872.t1
chr3.1 exon 52611941 52613044 52611941 ID=MS.gene53872.t1.exon3;Parent=MS.gene53872.t1
chr3.1 CDS 52611941 52613044 52611941 ID=cds.MS.gene53872.t1;Parent=MS.gene53872.t1
chr3.1 exon 52611360 52611839 52611360 ID=MS.gene53872.t1.exon4;Parent=MS.gene53872.t1
chr3.1 CDS 52611360 52611839 52611360 ID=cds.MS.gene53872.t1;Parent=MS.gene53872.t1
Gene Sequence

>MS.gene53872

ATGGCGGCTAATCCTAATAGACTCTCTCTTGCCATGGAGAGAACTGGCCAATGGTACACAACACACTAAACTCAACTCTATTCCATTTTCAAATCCATGCATGCATCATACACACGCTTAACCATTTTCTATTTTGTACTTTGATTATTATTCCAGGGTTTTCTCTCAAGAAGTTCCAACTGATGTTATAGTTGAAGTAGGAGAAGCTCGTTTTTGCCTCCACAAGGTATCCAAAAAATGACCCATGTGAATTATATTGTTCATAGATTAATGTGTTTACTTGCGCCACCTGTGATCGAAGGCTTGTCTGGTGTTCGACATGTGTTAGTGTCCAACATCAATAAATGTGATTACATGTGTCGGTATTAGTTTGATAGCTTGTTTAGTTGTTTTAGTTCATATGATTATTGTGAAATTGTATTGAAGTTTTGAATATGATTGCAGTTCATGTTGGTGGCAAAGAGCAACTACATCAGAAAACTAATAATGGAATCAGATGAGACTCATCTCACAAGAATAGATCTCTCTGACATACCAGGAGGTCCTGGAATCTTTGAGAAAGCAGCTAAATTCTGTTACGGCGTCAACTTCGAGATAACGGTCCATAATGTCGCAGTTTTACGATGTGCAGCAGAGTTTCTCCAAATGACTGATCAGTATTGTGACAACAACCTTGCTGGCAGGACCGACGAATTTCTCTCTAAAGTCGCCTTCTTCACTCTCACTGGCTCCGTTGCAGTCCTCAAGTCTTGCCACCACCTTCTTCCCTATGCCGATGACCTAAACATCGTGAAACGCTGCGTTGAAGCTGCAAGCGCCAAGGCTTGCAGCGAGGCGAATTTCCCTAGCCGTTCACCCGCGAATTGGTGGACTGAGGAACTAGCTGTTCTTGATATTGATTTCTTCAGTAGAGTGATCGCATGTATGAAACAGCGCGGCGCTAAGCCTCCAACCATTGCAGCCGCGCTGATTACATACTCAGAGCGATCACTCCAGGAGCTAGTTCGAAACCACTCCGGTAATGGAATCCAGTCATCAGATTACGGTGATTCAGACATCAGATCAAAACAACGAAGCCTTCTAGAATCAATTATCAATCTCTTCCCGTCCGAGAAAGCTGCTTTTCCGATAAACTTCCTCTGCTGCCTCCTCCGTTGCGCAATCCACCTCCGTGCCTCCACCGTCTGCAAAACCGAACTCGAGAAACGAATCTCAGCAATCCTTGAACACGTAACCGTTGACGACCTCCTCGTGATGTCATTCTCCTACGACGGCGAGAAACTCTTCGATCTAGAAAGCGTTCGCAGAATCATCTCAGTATTCGTTGAAAAGGAGAAAAACGCTGCAGTTTTCACACCGGGAGAACTCAAAGAATCATTCTCCGTCGCTATGCTACGTGTGGCGAAAACCGTCGACATGTACCTCGCTGAAATCGCCGCGTACGGTGAACTCAGCATCTCAAAGTTCAACGGAATCGCTGTTCTTATTCCTAAACACGCGCGTAAAATCGACGATGATCTTTACCGCGCCGTTGATATCTATCTCAAGGTAAAAACAAAGAATACCTTAACAAATTTGGATTTGAATTTCATCGTTCAATTTTATGATGAAAATGACACCGTTTAAACGAGTTTGTATATTTTTTTCAGGTTCATCCAAAGCTAGATGAAATCGAAAGAGAAAAAGTATGCAGCATAATGGATCCATTGAAACTATCCTACGAAGCGCGTGTGCACGCATCACAGAATAAACGGTTACCAGTGCAGATTGTTTTACACGCGCTATACTACGATCAGTTGAAGTTGAGGAGTGGATCAGACGACGGCGAATGCGACGGAGCTGCGACGGAGAGAAATCGTTTAGCGACGGATGTGTCATTGGTGAGAGAGAATGAGGAGTTACGATCAGAATTAACGAAGATGAAGATGTACATTACGGATATGCAGCAGCAGAAGAACGTTACGCCTGGAACGACGTCGTTGAGTTCGAGTAAGAAGACAACGTTTTTTTCTTCCGTGTCGAAAAAACTGGGAAAGTTGTTCAAAAATGGGGCTAAGGATACGGCCCATTTAGAAGATGGGCCTGTGGATTTGACTAAGCCCAGGAGAAGAAGGTTCTCTATTTCTTAA
Protein sequence

>MS.gene53872.t1

MAANPNRLSLAMERTGQWVFSQEVPTDVIVEVGEARFCLHKFMLVAKSNYIRKLIMESDETHLTRIDLSDIPGGPGIFEKAAKFCYGVNFEITVHNVAVLRCAAEFLQMTDQYCDNNLAGRTDEFLSKVAFFTLTGSVAVLKSCHHLLPYADDLNIVKRCVEAASAKACSEANFPSRSPANWWTEELAVLDIDFFSRVIACMKQRGAKPPTIAAALITYSERSLQELVRNHSGNGIQSSDYGDSDIRSKQRSLLESIINLFPSEKAAFPINFLCCLLRCAIHLRASTVCKTELEKRISAILEHVTVDDLLVMSFSYDGEKLFDLESVRRIISVFVEKEKNAAVFTPGELKESFSVAMLRVAKTVDMYLAEIAAYGELSISKFNGIAVLIPKHARKIDDDLYRAVDIYLKVHPKLDEIEREKVCSIMDPLKLSYEARVHASQNKRLPVQIVLHALYYDQLKLRSGSDDGECDGAATERNRLATDVSLVRENEELRSELTKMKMYITDMQQQKNVTPGTTSLSSSKKTTFFSSVSKKLGKLFKNGAKDTAHLEDGPVDLTKPRRRRFSIS