AlfalfaGEDB | MS.gene049600

M. sativa cultivar XinJiangDaYe / MS.gene049600

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: PF00651.34 PF03000.17

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene049600.t1	XP_003600550.1	97.7	568	13	0	1	568	1	568	0.00E+00	1093.6

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene049600.t1	Q682S0	65.8	584	181	6	4	568	10	593	1.3e-214	747.3

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene049600.t1	G7IV50	97.7	568	13	0	1	568	1	568	0.0e+00	1093.6

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049232	MS.gene049600	0.84588	3.08E-59	-1.69E-46
MS.gene049320	MS.gene049600	0.816762	4.68E-52	-1.69E-46
MS.gene049600	MS.gene05010	0.80275	4.84E-49	-1.69E-46
MS.gene049600	MS.gene050496	0.800918	1.15E-48	-1.69E-46
MS.gene049600	MS.gene050502	0.80833	3.27E-50	-1.69E-46
MS.gene049600	MS.gene050504	0.810415	1.17E-50	-1.69E-46
MS.gene049600	MS.gene050518	0.802423	5.65E-49	-1.69E-46
MS.gene049600	MS.gene050986	0.818514	1.88E-52	-1.69E-46
MS.gene049600	MS.gene05118	0.95078	8.10E-109	-1.69E-46
MS.gene049600	MS.gene051509	0.880986	3.49E-70	-1.69E-46

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PPI:

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene049600.t1	MTR_3g062540	97.711	568	13	0	1	568	1	568	0.0	1150
MS.gene049600.t1	MTR_3g062540	97.529	526	13	0	43	568	1	526	0.0	1060
MS.gene049600.t1	MTR_5g075640	72.069	580	142	7	4	568	5	579	0.0	833
MS.gene049600.t1	MTR_5g075640	71.719	541	133	7	43	568	1	536	0.0	763
MS.gene049600.t1	MTR_5g016060	40.065	614	306	10	8	566	24	630	2.17e-143	428
MS.gene049600.t1	MTR_4g095430	38.424	609	309	10	12	568	1	595	6.01e-135	405
MS.gene049600.t1	MTR_4g022330	38.889	576	299	10	8	539	16	582	2.09e-133	402
MS.gene049600.t1	MTR_1g088885	36.174	622	317	16	7	568	20	621	3.81e-122	373
MS.gene049600.t1	MTR_7g108290	34.891	599	315	12	7	547	20	601	3.53e-115	357
MS.gene049600.t1	MTR_7g073980	37.500	552	305	10	23	539	32	578	4.32e-114	353

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene049600.t1	AT2G30520	66.781	584	175	6	4	568	10	593	0.0	792
MS.gene049600.t1	AT2G30520	66.606	545	163	6	43	568	1	545	0.0	730
MS.gene049600.t1	AT2G30520	66.606	545	163	6	43	568	1	545	0.0	730
MS.gene049600.t1	AT5G67385	38.274	614	320	13	2	568	3	604	8.72e-129	390
MS.gene049600.t1	AT5G48800	38.079	583	286	10	7	539	22	579	4.74e-128	388
MS.gene049600.t1	AT1G03010	35.522	594	309	13	7	539	20	600	2.94e-119	366
MS.gene049600.t1	AT1G03010	35.813	578	298	12	23	539	8	573	5.43e-118	362
MS.gene049600.t1	AT1G03010	35.813	578	298	12	23	539	34	599	2.24e-117	361
MS.gene049600.t1	AT2G47860	36.559	558	282	11	7	509	21	561	1.89e-112	348
MS.gene049600.t1	AT1G30440	34.510	623	328	13	11	555	12	632	8.14e-111	345

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Find 119 sgRNAs with CRISPR-Local

Find 156 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGCAGCTAAATTCTGTTA+CGG	0.532489	3.2:-58773277	MS.gene049600:CDS
AAGCGCCAAGGCTTGCAGCG+AGG	0.692127	3.2:-58773023	MS.gene049600:CDS
AATAGAGAACCTTCTTCTCC+TGG	0.481171	3.2:+58771736	None:intergenic
AATATGATTGCAGTTCATGT+TGG	0.613034	3.2:-58773404	MS.gene049600:intron
AATCACTGTAATCTGATGAC+TGG	0.550628	3.2:+58772807	None:intergenic
AATGATTCTTTGAGTTCTCC+CGG	0.510540	3.2:+58772479	None:intergenic
AATTATCAATCTCTTCCCAT+CGG	0.625194	3.2:-58772747	MS.gene049600:CDS
AATTCGCGGGTGAGCGGCTA+GGG	0.462163	3.2:+58772993	None:intergenic
ACCCGCGAATTGGTGGACTG+AGG	0.708374	3.2:-58772981	MS.gene049600:CDS
ACTACGATCAGTTGAAGTTG+AGG	0.608135	3.2:-58772051	MS.gene049600:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAGCAGCTAAATTCTGTTA+CGG	-	chr3.2:58772335-58772354	MS.gene049600:CDS	30.0%
AATATGATTGCAGTTCATGT+TGG	-	chr3.2:58772208-58772227	MS.gene049600:intron	30.0%
AATTATCAATCTCTTCCCAT+CGG	-	chr3.2:58772865-58772884	MS.gene049600:CDS	30.0%
ACTGATGTTATAGTTGAAGT+TGG	-	chr3.2:58771948-58771967	MS.gene049600:CDS	30.0%
ACTTCAACTATAACATCAGT+TGG	+	chr3.2:58771948-58771967	None:intergenic	30.0%
GTAATCACACTTATTGATGT+TGG	+	chr3.2:58772106-58772125	None:intergenic	30.0%
TACCTTGAGATAGATATCAA+CGG	+	chr3.2:58773308-58773327	None:intergenic	30.0%
TGGGAAAGTTGTTCAAAAAT+GGG	-	chr3.2:58773804-58773823	MS.gene049600:intron	30.0%
AATCACTGTAATCTGATGAC+TGG	+	chr3.2:58772808-58772827	None:intergenic	35.0%
ATAGCAAGTGAGAGTCTATT+AGG	+	chr3.2:58771745-58771764	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr3.2	gene	58771730	58773904	58771730	ID=MS.gene049600
chr3.2	mRNA	58771730	58773904	58771730	ID=MS.gene049600.t1;Parent=MS.gene049600
chr3.2	exon	58773852	58773904	58773852	ID=MS.gene049600.t1.exon1;Parent=MS.gene049600.t1
chr3.2	CDS	58773852	58773904	58773852	ID=cds.MS.gene049600.t1;Parent=MS.gene049600.t1
chr3.2	exon	58773639	58773708	58773639	ID=MS.gene049600.t1.exon2;Parent=MS.gene049600.t1
chr3.2	CDS	58773639	58773708	58773639	ID=cds.MS.gene049600.t1;Parent=MS.gene049600.t1
chr3.2	exon	58772310	58773413	58772310	ID=MS.gene049600.t1.exon3;Parent=MS.gene049600.t1
chr3.2	CDS	58772310	58773413	58772310	ID=cds.MS.gene049600.t1;Parent=MS.gene049600.t1
chr3.2	exon	58771730	58772209	58771730	ID=MS.gene049600.t1.exon4;Parent=MS.gene049600.t1
chr3.2	CDS	58771730	58772209	58771730	ID=cds.MS.gene049600.t1;Parent=MS.gene049600.t1

Gene Sequence

>MS.gene049600

ATGGCGGCTAATCCTAATAGACTCTCACTTGCTATGGAGAGAACTGGCCAATGGTACACAACACACTAAACAACTCTTTCCATTTTCAAATCCATGCATGCATCATCATACACATGCTTACTCTACAATTAATCTAATTTAATCTTTACCATATGTAATAACCATTTTATATTTTGTACTTTGATTATTATTGCAGGGTTTTCTCTCAAGAAGTTCCAACTGATGTTATAGTTGAAGTTGGAGAAGCTCGTTTCTGCCTCCACAAGGTATATCCAAAAAATGACCCATGTGAATTATATTGTTCATAGGTTAATGTGTTTACTTGCGCCGCTTGTGATCGAAGGCTTGTCTGGTGTTCGACATGTGTTAGTGTCCAACATCAATAAGTGTGATTACATGCGTCAGTATTAGAATCGTGTCGGTGATTGATAGTTTGTTTTAGTTCATATGATTATTGTAAAATTGTATTGAAGTTTTGAATATGATTGCAGTTCATGTTGGTGGCAAAGAGCAACTACATCAGAAAACTAATAATGGAATCAGATGAGACTCATCTCACCAGAATAGATCTCTCTGACATACCAGGAGGTCCTGGAATCTTTGAGAAAGCAGCTAAATTCTGTTACGGCGTCAACTTCGAGATAACCGTCCATAATGTCGCAGTTTTACGATGTGCTGCAGAGTTTCTCCAAATGACTGATCAGTATTGTGACAACAATCTCGCTGGCCGGACCGATGAATTTCTCTCTAAGGTCGCCTTTTTCACTCTCACCGGCTCCGTTGCAGTCCTCAAGTCTTGCCACCACCTTCTGCCCTATGCTGATGACCTAAACATCGTCAAACGCTGCGTTGAAGCTGCAAGCGCCAAGGCTTGCAGCGAGGCGAATTTCCCTAGCCGCTCACCCGCGAATTGGTGGACTGAGGAACTAGCTGTTCTTGATATTGATTTCTTCAGTAGAGTGATCACATGTATGAAACAGCGCGGGGCTAAGCCTCCAACCATTGCAGCCGCGCTGATTACATACTCAGAGCGATCCCTCCAGGAGCTAGTTCAAAACCACTCCGGTAACGGAATCCAGTCATCAGATTACAGTGATTCAGACATTAGATCAAAACAAAGAAGCCTTCTAGAATCAATTATCAATCTCTTCCCATCGGAGAAAGCAGCTTTTCCGATCAACTTCCTCTGCTGCCTCCTCCGTTGCGCAATCCACCTCCGTGCCTCCACAGTCTGCAAAAATGAACTCGAGAAACGAATCTCAGCAATCCTTGAACACGTAACCGTCGATGACCTCCTCGTGATGTCATTCTCCTACGACGGCGAGAAACTCTTCGATCTAGAAAGCGTTCGCAGAATCATCTCAGTATTCGTTGAAAAGGAGAAAAACGCTGCAGTTTTCACACCGGGAGAACTCAAAGAATCATTCTCCGTCGCTATGCTACATGTGGCGAAAACCGTCGACATGTACCTCGCTGAAATAGCCGCGTACGGCGAACTCAGCATCTCAAAGTTCAATGGAATCGCTGTTCTTATTCCTAAACACGCGCGTAAAATCGACGATGATCTTTACCGCGCCGTTGATATCTATCTCAAGGTAAAAACAAAAAACATCTTAACAAATTTGAATTTAAATTTCATCGTTCAATTTTATCATGAAAACGTCACTGTTTAAACGAGTTTGTTTATCTCTGCAGGTTCATCCGAAGCTAGATGAAATCGAAAGAGAGAAAGTATGCAGCATAATGGATCCATTGAAGCTATCATACGAAGCGCGTGTACACGCGTCGCAGAACAAACGGTTACCGGTGCAGATAGTTTTACACGCGCTATACTACGATCAGTTGAAGTTGAGGAGTGGATCAGACGATGGCGAATGCGACGGAGCTGCGACGGAGAGGAATCGGTTAGCGACGGATGTGTCGTTGGTGAGAGAGAATGAGGAGTTACGATCGGAATTGACGAAGATGAAAATGTACATTACGGATATGCAGCAGCAGAAGAACGTTACGCCGGGAACGACGTCGTTGAGTTCGAGTAAGAAGACGACGTTTTTTTCTTCCGTGTCGAAAAAACTGGGAAAGTTGTTCAAAAATGGGGCTAAGGATACGGCCCATTTAGAAGATGGGCCTGTGGATTTGACTAAGCCCAGGAGAAGAAGGTTCTCTATTTCTTAA

Protein sequence

>MS.gene049600.t1

MAANPNRLSLAMERTGQWVFSQEVPTDVIVEVGEARFCLHKFMLVAKSNYIRKLIMESDETHLTRIDLSDIPGGPGIFEKAAKFCYGVNFEITVHNVAVLRCAAEFLQMTDQYCDNNLAGRTDEFLSKVAFFTLTGSVAVLKSCHHLLPYADDLNIVKRCVEAASAKACSEANFPSRSPANWWTEELAVLDIDFFSRVITCMKQRGAKPPTIAAALITYSERSLQELVQNHSGNGIQSSDYSDSDIRSKQRSLLESIINLFPSEKAAFPINFLCCLLRCAIHLRASTVCKNELEKRISAILEHVTVDDLLVMSFSYDGEKLFDLESVRRIISVFVEKEKNAAVFTPGELKESFSVAMLHVAKTVDMYLAEIAAYGELSISKFNGIAVLIPKHARKIDDDLYRAVDIYLKVHPKLDEIEREKVCSIMDPLKLSYEARVHASQNKRLPVQIVLHALYYDQLKLRSGSDDGECDGAATERNRLATDVSLVRENEELRSELTKMKMYITDMQQQKNVTPGTTSLSSSKKTTFFSSVSKKLGKLFKNGAKDTAHLEDGPVDLTKPRRRRFSIS